Tobacco extract composition, preparation method therefor, and use thereof

Abstract

A tobacco extract composition, a preparation method therefor, and a use thereof, relating to the technical field of electronic atomization. The tobacco extract composition comprises methyl cyclopentenolone, guaiacol, neophytadiene, a cembratriene-diol, dihydroactinidiolide, maltol, furanone, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one, and scopoletin. By weight: (methyl cyclopentenolone + guaiacol) / neophytadiene ranges from 0.01 to 2.5. By dry weight: the guaiacol content is 0.003 to 0.02 mg / g. Said tobacco has a rich roasted aroma, a pronounced sweet roasted flavor, and a more tobacco-like feel. The preparation method comprises: performing extraction on tobacco raw material twice at medium and low temperatures, and obtaining tobacco flavor compounds; performing extraction on the tobacco raw material at a high temperature, and obtaining smoke flavor compounds; and mixing the tobacco flavor compounds and the smoke flavor compounds in a certain ratio to obtain a tobacco extract composition.

Description

A tobacco extract composition, its preparation method and application

[0001] Cross-references to related applications

[0002] This application claims priority to Chinese Patent Application No. 2024119939833, filed on December 30, 2024, entitled "A Tobacco Extract Composition and Its Preparation Method and Application", the entire contents of which are incorporated herein by reference. Technical Field

[0003] This application belongs to the field of electronic atomization technology, specifically relating to a tobacco extract composition, its preparation method, and its application. Background Technology

[0004] Electronic atomizing devices are electronic products that mimic cigarettes. When people inhale electronic atomizing devices, they inhale an electronic atomizing liquid. Compared to traditional cigarettes, electronic atomizing devices do not require combustion and do not produce harmful substances such as combustion byproducts, meaning they have a lower content of harmful substances compared to traditional cigarettes. However, existing electronic atomizing devices often lack appeal to long-time smokers due to insufficient aroma and poor "smoke-like" experience. Improving the "smoke-like" experience and thus increasing the acceptance of electronic atomizing devices by long-time smokers is a pressing issue that needs to be addressed. Summary of the Invention

[0005] Therefore, the technical problem to be solved by this application is to overcome the defects of insufficient tobacco aroma and poor real tobacco sensation in the prior art of electronic atomization devices, thereby providing a tobacco extract composition, its preparation method and application.

[0006] Therefore, this application provides the following technical solution.

[0007] This application provides a tobacco extract composition comprising methylcyclopentenolone, guaiacol, neophytadiene, cephalotrimethylenediol, dihydroactinol, maltol, furanone, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one, and scopolamine; the ratio of (methylcyclopentenolone + guaiacol) / neophytadiene by weight is in the range of 0.01 to 2.5; and the guaiacol content by dry matter in the tobacco extract composition is 0.003 to 0.02 mg / g.

[0008] Optionally, the ratio of (methylcyclopentenolone + guaiacol) / neoptiene, by mass, ranges from 0.02 to 2.5.

[0009] Optionally, the tobacco extract composition further includes at least one of nicotine, megastigmatrienone, spirovestigma, and furfuryl alcohol.

[0010] In this application, "dry matter" refers to the components in the tobacco extract composition other than the solvent. The solvent may be an aqueous ethanol solution, and the dry matter includes: methylcyclopentenolone, guaiacol, neophytadiene, cephalotrimazole, dihydroactinol, maltol, furanone, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one, scopolamine, and may also include: nicotine, megastigmatrienone, spirovestilbene, and furfuryl alcohol.

[0011] Optionally, the tobacco extract composition satisfies at least one of the following conditions, with each content calculated based on the dry matter content of the tobacco extract composition:

[0012] (1) The content of cipertrienol is ≥0.1 mg / g; optionally, the content of cipertrienol is 0.55~3.5 mg / g;

[0013] (2) The content of 6-ethyl-5,6-dihydro-2H-pyran-2-one is ≥0.014 mg / g; optionally, the content of 6-ethyl-5,6-dihydro-2H-pyran-2-one is 0.015~0.3 mg / g;

[0014] (3) The content of megastigmatrienone is ≥0.12 mg / g; optionally, the content of megastigmatrienone is 0.12 to 0.6 mg / g;

[0015] (4) Palmitic acid content ≥ 0.011 mg / g; optionally, palmitic acid content is 0.011–0.65 mg / g;

[0016] (5) Scopolamine content ≥ 0.008 mg / g; optionally, scopolamine content is 0.01~0.20 mg / g;

[0017] (6) Neophytadiene content ≥ 0.012 mg / g; optionally, neophytadiene content is 0.012–0.12 mg / g;

[0018] (7) The content of dihydroactinol is ≥0.01mg / g; optionally, the content of dihydroactinol is 0.01~0.055mg / g;

[0019] (8) Spirophyrin content ≥ 0.01 mg / g; optionally, spirophyrin content is 0.01–0.2 mg / g;

[0020] (9) The maltol content is 0.0011~0.065mg / g;

[0021] (10) The furanone content is 0.008 to 0.080 mg / g; optionally, the furanone includes at least one of 2(H) furanone and 5(H) furanone;

[0022] (11) The content of methylcyclopentenolone is 0.003~0.03mg / g;

[0023] (12) The furfuryl alcohol content is 0.005~0.08mg / g;

[0024] (13) Nicotine content ≥ 1.0 mg / g; optionally, nicotine content is 1.0 to 10.0 mg / g.

[0025] The functions of each component in the tobacco extract composition of this application are as follows:

[0026] The presence of nicotine brings greater satisfaction.

[0027] Cembratriene-diols (CAS: 57605-80-8) can enhance the aroma of tobacco and release aroma-producing substances such as solanone and solanesol when heated.

[0028] 6-Ethyl-5,6-dihydro-2H-pyran-2-one (CAS: 19895-35-3) is an important flavor intermediate. When heated above 200°C, it can release furanone, maltol, and other components that bring out the roasted sweetness.

[0029] Megastigmatrienone (CAS: 38818-55-2) can impart a tobacco hay aroma.

[0030] Palmitic acid (n-Hexadecanoic acid / CAS: 57-10-3) can smooth out smoke and increase its mellowness.

[0031] Scopolone (CAS: 92-61-5) can increase tobacco satisfaction and pleasure.

[0032] The main function of neophytadiene (CAS: 504-96-1) is to enhance the aroma of tobacco, promote its permeability, increase the duration of the tobacco aroma, and make the overall aroma more harmonious.

[0033] Dihydroactinidiolide (CAS: 17092-92-1) primarily enhances the aroma and sweetness of tobacco.

[0034] Solavetivone (CAS: 54878-25-0) can enhance the aroma of tobacco hay.

[0035] Maltol (CAS: 118-71-8) primarily enhances the caramelized sweetness.

[0036] Furanol (2(H)+5(H)) (Furanol / CAS: 3658-77-3&497-23-4) is mainly produced through high temperature and is the main source of the sweetness and caramelization of tobacco.

[0037] Methylcyclopentenolone (CAS: 80-71-7) primarily enhances the caramel sweetness.

[0038] Methylenecyclopropanecarboxylic acid (CAS: 98-00-0) can impart a tobacco roasted aroma and fresh fragrance, but it is easily converted into furfural by heating.

[0039] Guaiacol (CAS: 90-05-1) can enhance the smoky flavor and improve the authentic smoking experience.

[0040] Optionally, the tobacco extract composition further satisfies at least one of the following conditions, with each content calculated based on the dry matter content of the tobacco extract composition:

[0041] A. Phenol content < 0.06 mg / g; optionally, phenol content < 0.05 mg / g;

[0042] B. Hydroxyacetone content < 0.05 mg / g; Optional, hydroxyacetone content < 0.055 mg / g;

[0043] C. Total nitrosamines < 0.05 mg / g; optionally, nitrosamines include N′-nitrosonornicotinine, 4-(N-methylnitroso)-1-(3-pyridyl)-1-butanone, N′-nitrosopseudoestiline, and N′-nitrosoneonicotinine; further optionally, total nitrosamines < 0.015 mg / g.

[0044] This application provides a method for preparing the above-mentioned tobacco extract composition, comprising the following steps:

[0045] S1: The tobacco raw material is extracted for the first time at 25-50°C to obtain the first extract. The extraction solvent used for the first extraction includes an 80wt%-95wt% aqueous ethanol solution. The first extract is then extracted for the second time at -20-0°C to obtain tobacco flavor compounds.

[0046] S2: Extract tobacco raw materials at 100-200℃, separate solid and liquid to obtain extract, treat extract with porous adsorption material to obtain tobacco flavor compounds;

[0047] S3: Mix the tobacco flavoring agent and the smoke flavoring agent at a mass ratio of 10:1 to 1:10 to obtain the tobacco extract composition.

[0048] Optionally, in step S1, the mass ratio of tobacco raw material to the extraction medium used in the first extraction is 1:10 to 15, and the first extraction time is 5 to 24 hours.

[0049] Optionally, in step S1, the mass ratio of the first extract to the extraction medium used in the second extraction is 1:2 to 15, and the second extraction time is 5 to 12 hours.

[0050] Optionally, in step S2, the mass of the extraction solvent used for extraction is 60% to 80% of the tobacco raw material, and the extraction time is 1 to 6 hours.

[0051] Optionally, in step S2, the mass of the porous adsorption material is 1.5% to 15% of the extract, and the time for treating the extract with the porous adsorption material is 2 to 6 hours.

[0052] Optionally, in step S1, the extraction medium used for the second extraction includes ethanol, and also includes at least one of water and propylene glycol; optionally, the concentration of ethanol in the extraction medium used for the second extraction is 80wt% to 95wt%.

[0053] Optionally, in step S2, the extraction method includes any one of microwave dynamic temperature field extraction, thermal reaction extraction, and dry distillation extraction.

[0054] Optionally, in step S2, the extraction solvent includes at least one of water, ethanol, propylene glycol, and glycerol.

[0055] Optionally, the tobacco raw material includes at least one of tobacco leaves, tobacco stems, tobacco powder, tobacco, tobacco waste, tobacco extract, and tobacco pure oil; optionally, when the tobacco raw material is solid, its size is ≤20 mesh.

[0056] Optionally, the porous adsorbent material includes at least one of activated carbon, zeolite, and diatomaceous earth.

[0057] In the method for preparing the tobacco extract composition provided in this application, the humidity of the first extraction in S1 is not particularly limited, and common ambient humidity is acceptable, such as 40% to 80%.

[0058] The method for preparing the tobacco extract composition provided in this application typically, but not limited to, includes, for ease of operation, a step of concentrating the obtained first extract, tobacco flavor compounds, and smoke flavor compounds (hereinafter collectively referred to as the respective mixtures) under reduced pressure after each extraction step. The temperature for reduced pressure concentration is 50–70°C, the pressure is 60–200 mbar, and the concentration is increased to a concentration of 50%–80%. The concentration refers to the mass percentage of the components other than the solvent in each respective mixture, and can be detected using a concentration meter. In step S1, the apparatus used for the first extraction includes at least one of a supercritical fluid extraction device, a reflux tank, a percolator, and a stirred extraction tank.

[0059] In the method for preparing the tobacco extract composition provided in this application, typically and non-limitingly, in step S1, after the second extraction, a solid-liquid separation step is further included to separate the obtained tobacco flavor compounds; the solid-liquid separation method includes centrifugation, with a centrifugation time of 10 to 30 minutes, a temperature of -5 to 5°C, and a rotation speed of 7000 to 9000 r.

[0060] In the method for preparing the tobacco extract composition provided in this application, typically and non-limitingly, before treating the extract with a porous adsorbent material in step S2, a step of removing impurities may also be included. The step of removing impurities includes: adding 2 to 4 times its mass of an aqueous ethanol solution (85wt% to 95wt%) to the extract, sonicating for 10 to 30 minutes to disperse it evenly, storing it at -25 to -0℃ for 8 to 16 hours, centrifuging for 10 to 30 minutes at centrifugation parameters of -5 to 5℃ and 7000 to 9000 rpm, and taking the supernatant obtained by centrifugation for subsequent steps.

[0061] In the preparation method of the tobacco extract composition provided in this application, the tobacco raw materials used are not particularly limited, such as burley tobacco, cigar tobacco, aromatic tobacco, etc.

[0062] This application provides an aerosol generating matrix, comprising a tobacco extract composition, and further comprising at least one of organic acid, flavoring, and atomizing agent; wherein the tobacco extract composition is the above-mentioned tobacco extract composition or a tobacco extract composition prepared by the above-mentioned preparation method.

[0063] Optionally, the aerosol generating matrix also satisfies at least one of the following conditions:

[0064] 1) The mass of the tobacco extract composition is 1% to 50% of the total aerosol-generating matrix;

[0065] 2) The mass of the atomizing agent is 50% to 99% of the total aerosol generating matrix mass; optionally, the atomizing agent includes at least one of propylene glycol, glycerol, water, and ethanol; ready-made PGVG can be used, wherein the mass ratio of propylene glycol to vegetable glycerol (i.e., glycerol) in the PGVG is 3:7 to 7:3.

[0066] 3) The organic acid accounts for 0-3% of the total aerosol-generating matrix; optionally, the organic acid includes at least one of acetic acid, lactic acid, benzoic acid, malic acid, tartaric acid, citric acid, and levulinic acid.

[0067] 4) The flavoring is 0-3% of the total aerosol-generating matrix; typically, without limitation, the flavoring type can be food flavoring or tobacco flavoring, which can be purchased directly or made at home.

[0068] 5) The aerosol generating matrix includes additional nicotine, and the content of the additional nicotine is 0 to 3% of the total mass of the aerosol generating matrix.

[0069] This application also provides the use of the above-described tobacco extract composition or the tobacco extract composition prepared by the above-described preparation method or the above-described aerosol generating matrix in an atomizing device.

[0070] The beneficial effects of this application are:

[0071] The tobacco extract composition provided in this application includes methylcyclopentenolone, guaiacol, neophytadiene, cephalotrimethylenediol, dihydroactinol, maltol, furanone, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one, and scopolamine; the ratio of (methylcyclopentenolone + guaiacol) to neophytadiene is in the range of 0.01 to 2.5 by mass; and the guaiacol content is 0.003 to 0.02 mg / g by dry matter weight in the tobacco extract composition. This tobacco extract composition has a richer tobacco roasted aroma, a more pronounced tobacco roasted sweetness, and a more tobacco-like feel.

[0072] This application provides a method for preparing the above-mentioned tobacco extract composition, comprising the following steps: S1: Extracting tobacco raw material for the first time at 25-50°C to obtain a first extract, wherein the extraction solvent used for the first extraction includes an 80wt%-95wt% aqueous ethanol solution; performing a second extraction on the first extract at -20-0°C to obtain tobacco flavor compounds; S2: Extracting tobacco raw material at 100-200°C, separating the solid and liquid to obtain an extract, treating the extract with a porous adsorption material to obtain a smoke flavor compound; S3: Mixing the tobacco flavor compound and the smoke flavor compound at a mass ratio of 10:1 to 1:10 to obtain the tobacco extract composition. The tobacco extract composition prepared by this method has a high content of characteristic tobacco flavor substances and contains certain smoke flavor substances to enhance the authentic tobacco flavor, while having a low content of harmful substances. Detailed Implementation

[0073] The following embodiments are provided to better understand this application and are not limited to the preferred embodiments described herein. They do not constitute a limitation on the content and scope of protection of this application. Any product that is the same as or similar to this application, derived by anyone under the guidance of this application or by combining features of this application with other prior art, falls within the scope of protection of this application.

[0074] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0075] The tobacco used in the embodiments and comparative examples of this application is Burley tobacco.

[0076] In this application, concentration refers to the mass percentage of the components other than the solvent in the corresponding mixture, which is detected using an ATAGO concentration meter.

[0077] Example 1

[0078] This embodiment provides a tobacco extract composition and its preparation method, including the following steps:

[0079] (1) Preparation of tobacco flavorings: The tobacco leaves were pulverized to 20 mesh and then extracted with 95 wt% ethanol at room temperature. The mass ratio of tobacco leaves to 95 wt% ethanol was 1:10. The specific extraction conditions were: extraction temperature 25℃, ambient humidity 60±10%, and extraction time 12h to obtain the first extract. The first extract was concentrated to 60% concentration under reduced pressure at 60℃ and 100mbar to obtain a concentrate. Five times the mass of 90 wt% ethanol aqueous solution was added to the concentrate, and the mixture was sonicated for 10min to disperse it evenly. After storage at -18℃ for 12h, the extract was centrifuged for 10min to achieve the second extraction. The centrifugation parameters were -4℃ and 8000r. After centrifugation, the supernatant was separated and concentrated to 80% concentration under reduced pressure at 60℃ and 100mbar to obtain the tobacco flavorings.

[0080] (2) Preparation of tobacco flavor compounds: Tobacco leaves were pulverized to 20 mesh, and 10% (by weight) of PG (propylene glycol) and 50% (by weight) of water were added. The mixture was placed in a stainless steel hot reactor and reacted naturally at 100°C for 1 hour. After cooling, the tobacco powder residue was filtered off to obtain the extract. Three times the weight of the extract (90 wt%) of ethanol aqueous solution was added, and the mixture was sonicated for 10 minutes to ensure uniform dispersion. The extract was stored at -18°C for 12 hours, then centrifuged for 10 minutes at -4°C and 8000 rpm. After centrifugation, the supernatant was separated. 5% (by weight) of activated carbon was added to the supernatant and allowed to stand for 3 hours. After filtering off the activated carbon, the extract was concentrated under reduced pressure at 60°C and 100 mbar to a concentration of 70%, which is the tobacco flavor compound.

[0081] (3) Combine the tobacco flavoring obtained in (1) with the smoke flavoring obtained in (2) at a mass ratio of 10:1 to obtain a tobacco extract composition.

[0082] Example 2

[0083] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 1 is that in step (3), tobacco flavorings and smoke flavorings are combined in a mass ratio of 1:1 to obtain the tobacco extract composition.

[0084] Example 3

[0085] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 1 is that in step (3), tobacco flavorings and smoke flavorings are combined at a mass ratio of 1:10 to obtain the tobacco extract composition.

[0086] Example 4

[0087] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 1 is that the reaction in step (2) is to place it in a microwave dynamic reaction extraction vessel and react naturally at 150°C for 1 hour.

[0088] Example 5

[0089] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 4 is that in step (3), tobacco flavorings and smoke flavorings are combined in a mass ratio of 1:1 to obtain the tobacco extract composition.

[0090] Example 6

[0091] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 4 is that in step (3), tobacco flavorings and smoke flavorings are combined at a mass ratio of 1:10 to obtain the tobacco extract composition.

[0092] Example 7

[0093] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 1 is that the reaction in step (2) is to place it in a dry distillation reaction extraction vessel and allow it to react naturally at 200°C for 1 hour.

[0094] Example 8

[0095] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 7 is that in step (3), tobacco flavorings and smoke flavorings are combined in a mass ratio of 1:1 to obtain the tobacco extract composition.

[0096] Example 9

[0097] This embodiment provides a tobacco extract composition and its preparation method. The only difference from Example 7 is that in step (3), tobacco flavorings and smoke flavorings are combined at a mass ratio of 1:10 to obtain the tobacco extract composition.

[0098] Example 10

[0099] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 50 parts of the tobacco extract composition obtained in Example 5, 30 parts of propylene glycol (PG), and 20 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0100] Example 11

[0101] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 10 parts of the tobacco extract composition obtained in Example 5, 45 parts of propylene glycol (PG), and 45 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0102] Example 12

[0103] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 1 part of the tobacco extract composition obtained in Example 5, 30 parts of propylene glycol (PG), and 69 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0104] Example 13

[0105] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 10 parts of the tobacco extract composition obtained in Example 2, 3 parts of nicotine, 2 parts of benzoic acid, 42.5 parts of propylene glycol (PG), and 42.5 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0106] Example 14

[0107] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 10 parts of the tobacco extract composition obtained in Example 2, 1 part of nicotine, 1 part of lactic acid, 3 parts of tobacco flavoring (purchased from Hasegawa Biotechnology Co., Ltd.), 42.5 parts of propylene glycol (PG), and 42.5 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0108] Example 15

[0109] This embodiment provides an aerosol generating matrix, the preparation method of which includes: taking 10 parts of the tobacco extract composition obtained in Example 2, 1 part of nicotine, 1 part of lactic acid, 44 parts of propylene glycol (PG), and 44 parts of vegetable glycerin (VG), and mixing them to obtain the aerosol generating matrix.

[0110] Example 16

[0111] This embodiment provides a tobacco extract composition and its preparation method, including the following steps:

[0112] (1) Preparation of tobacco flavorings: The tobacco leaves were pulverized to 20 mesh and then extracted with 80 wt% ethanol at room temperature. The mass ratio of tobacco leaves to 80 wt% ethanol was 1:15. The specific extraction conditions were: extraction temperature 50℃, ambient humidity 60±10%, and extraction time 5h to obtain the first extract. The first extract was concentrated to 60% concentration under reduced pressure at 60℃ and 100mbar to obtain a concentrate. 15 times the mass of the concentrate was added to the concentrate, and the mixture was sonicated for 10min to disperse it evenly. After storage at -20℃ for 5h, it was centrifuged for 10min to achieve the second extraction. The centrifugation parameters were -4℃ and 8000r. After centrifugation, the supernatant was separated and concentrated to 80% concentration under reduced pressure at 60℃ and 100mbar to obtain the tobacco flavorings.

[0113] (2) Preparation of tobacco flavor compounds: Tobacco leaves were pulverized to 20 mesh, and 20% (by weight) of PG (propylene glycol) and 60% (by weight) of water were added. The mixture was placed in a stainless steel hot reactor and reacted naturally at 100°C for 6 hours. After cooling, the tobacco powder residue was filtered off to obtain the extract. Three times the weight of the extract (90 wt% ethanol aqueous solution) was added, and the mixture was sonicated for 10 minutes to ensure uniform dispersion. The extract was stored at -18°C for 12 hours, then centrifuged for 10 minutes at -4°C and 8000 rpm. The supernatant was separated after centrifugation. 15% (by weight) of activated carbon was added to the supernatant and allowed to stand for 2 hours. After filtering off the activated carbon, the extract was concentrated under reduced pressure at 60°C and 100 mbar to a concentration of 70%, which is the tobacco flavor compound.

[0114] (3) Combine the tobacco flavoring obtained in (1) with the smoke flavoring obtained in (2) at a mass ratio of 10:1 to obtain a tobacco extract composition.

[0115] Example 17

[0116] This embodiment provides a tobacco extract composition and its preparation method, including the following steps:

[0117] (1) Preparation of tobacco flavorings: The tobacco leaves were pulverized to 20 mesh and then extracted with 95 wt% ethanol at room temperature. The mass ratio of tobacco leaves to 80 wt% ethanol was 1:15. The specific extraction conditions were: extraction temperature 35℃, ambient humidity 60±10%, and extraction time 24 h to obtain the first extract. The first extract was concentrated to 60% concentration under reduced pressure at 60℃ and 100 mbar to obtain a concentrate. Twice the mass of the concentrate was added to the concentrate, and the mixture was sonicated for 10 min to disperse it evenly. After storage at -10℃ for 10 h, the extract was centrifuged for 10 min to achieve the second extraction. The centrifugation parameters were -4℃ and 8000 r. After centrifugation, the supernatant was separated and concentrated to 80% concentration under reduced pressure at 60℃ and 100 mbar to obtain the tobacco flavorings.

[0118] (2) Preparation of tobacco flavor compounds: Tobacco leaves were pulverized to 20 mesh, and 15% (by weight) of PG (propylene glycol) and 55% (by weight) of water were added. The mixture was placed in a stainless steel hot reactor and reacted naturally at 100°C for 3 hours. After cooling, the tobacco powder residue was filtered off to obtain the extract. Three times the weight of the extract (90 wt%) of ethanol aqueous solution was added, and the mixture was sonicated for 10 minutes to ensure uniform dispersion. The extract was stored at -18°C for 12 hours, then centrifuged for 10 minutes at -4°C and 8000 rpm. The supernatant was separated after centrifugation. 1.5% (by weight) of activated carbon was added to the supernatant and allowed to stand for 6 hours. After filtering off the activated carbon, the extract was concentrated under reduced pressure at 60°C and 100 mbar to a concentration of 70%, which is the tobacco flavor compound.

[0119] (3) Combine the tobacco flavoring obtained in (1) with the smoke flavoring obtained in (2) at a mass ratio of 10:1 to obtain a tobacco extract composition.

[0120] Comparative Example 1

[0121] This comparative example provides a tobacco extract, which differs from Example 4 in that only step (1) of Example 4 is performed, and the resulting tobacco flavor compound is the tobacco extract in this comparative example.

[0122] Comparative Example 2

[0123] This comparative example provides a tobacco extract, which differs from Example 4 in that only step (2) of Example 4 is performed, and the resulting smoke flavor is the tobacco extract in this comparative example.

[0124] Comparative Example 3

[0125] This comparative example provides a tobacco extract composition and its preparation method. The difference from Example 1 is that step (2) is as follows: Preparation of tobacco flavoring: The tobacco leaves are crushed to 20 mesh, 10% of PG (propylene glycol) and 50% of water by weight of the tobacco leaves are added, and the mixture is placed in a stainless steel hot reactor and reacted naturally at 80°C for 1 hour. After cooling, the tobacco powder residue is filtered off to obtain the extract. 90wt% ethanol aqueous solution with 3 times its weight is added to the extract, and the mixture is sonicated for 10 minutes to disperse it evenly. After storage at -18°C for 12 hours, it is taken out and centrifuged for 10 minutes. The centrifugation parameters are -4°C and 8000r. After centrifugation, the supernatant is separated. The supernatant is concentrated under reduced pressure at 60°C and 100mbar to a concentration of 70%, which is the tobacco flavoring. Step (3) is as follows: The tobacco flavoring obtained in (1) and the tobacco flavoring obtained in (2) are combined at a mass ratio of 1:1 to obtain the tobacco extract composition.

[0126] Comparative Example 4

[0127] This comparative example provides a tobacco extract composition and its preparation method. The difference from Example 7 is that step (2) is as follows: Preparation of tobacco flavoring: The tobacco leaves are crushed to 20 mesh, 10% of PG (propylene glycol) and 50% of water by weight of the tobacco leaves are added, and the mixture is placed in a dry distillation reaction vessel and reacted naturally at 250°C for 1 hour. After cooling, the tobacco powder residue is filtered off to obtain the extract. 90wt% ethanol aqueous solution with 3 times its weight is added to the extract, and the mixture is sonicated for 10 minutes to disperse it evenly. After storage at -18°C for 12 hours, it is taken out and centrifuged for 10 minutes. The centrifugation parameters are -4°C and 8000r. After centrifugation, the supernatant is separated. The supernatant is concentrated under reduced pressure at 60°C and 100mbar to a concentration of 70%, which is the tobacco flavoring. Step (3) is as follows: The tobacco flavoring obtained in (1) and the tobacco flavoring obtained in (2) are combined at a mass ratio of 1:1 to obtain the tobacco extract composition.

[0128] Comparative Example 5

[0129] This comparative example provides a tobacco extract composition and its preparation method. Compared with Example 4, the difference is that in step (1), 40wt% ethanol is used for extraction at room temperature; in step (2), the step of adding activated carbon to the supernatant is not included, and the supernatant is directly concentrated under reduced pressure; in step (3), the tobacco flavoring obtained in (1) and the smoke flavoring obtained in (2) are combined at a mass ratio of 1:1 to obtain the tobacco extract composition.

[0130] Test Example 1 (Component Content Detection)

[0131] Flavor component analysis was performed on the final products obtained from Examples 1-9, 16, and 17, which were comparative examples.

[0132] Take 1 mL of the final product and add dichloromethane at a volume ratio of 1:10 to extract the product. Concentrate the extract to separate the dichloromethane. Dissolve the residue in 1 mL of anhydrous ethanol and filter it through a 0.45 μm filter membrane for later use.

[0133] Detection procedure: Gas chromatography-mass spectrometry (GC-MS (6590-7890)) was used. The injection port temperature was 250℃, the initial temperature was 40℃, and it was held for 2 min; the temperature was increased to 150℃ at 3℃ / min and held for 5 min; the temperature was increased to 300℃ at 5℃ / min and held for 5 min; finally, the temperature was increased to 320℃ at 10℃ / min and held for 10 min. The relative content of each substance in the corresponding final product, based on dry matter mass, was calculated by peak area integration. The results are shown in Tables 1-3.

[0134] Table 1

[0135] Table 2

[0136] Table 3

[0137] Test Example 2 (Sensory Assessment)

[0138] Sensory evaluations were performed on the final products obtained from the examples and comparative examples.

[0139] The final products obtained in the examples and comparative examples were packaged into the same type of cartridge (T65B-1); if the final product is a tobacco extract composition, the atomizing liquid was first prepared by mass percentage of 10% tobacco extract composition and 90% PGVG (5:5).

[0140] Five expert evaluators conducted a blind sensory evaluation using the same model of electronic atomizer (RELX 5th generation). The vaping operation followed the general sensory evaluation standards for electronic atomizers (3 seconds of vaping per sample, with an interval of at least 10 minutes between samples). Scoring ranged from 1 to 10 points, using a positive scoring principle. The scoring dimensions emphasized the intensity of the tobacco aroma, the naturalness and tobacco-like sensation, and a comprehensive sensory evaluation. The intensity of the tobacco aroma primarily focused on the richness of the burnt tobacco flavor. Single sensory dimension (x) scoring criteria: 10 points maximum, x ≤ 3 points - weak, 3 < x < 7 points - average, x ≥ 7 points - strong. Overall sensory dimension (y) scoring criteria: 10 points maximum, y ≤ 3 points - poor, 3 < y < 7 points - average, y ≥ 7 points - excellent. The average scores were calculated and presented in Table 4.

[0141] Table 4

[0142] As can be seen from Tables 1-4, compared with the examples, the tobacco extract compositions obtained in the comparative examples do not meet the requirements of this application, which specify that the ratio of (methylcyclopentenolone + guaiacol) to neophytadiene is 0.01-2.5 by mass and the guaiacol content is 0.003-0.02 mg / g. A ratio of (methylcyclopentenolone + guaiacol) to neophytadiene below 0.01 and a guaiacol content below 0.003 mg / g will result in insufficient smoky sensation; while a ratio of (methylcyclopentenolone + guaiacol) to neophytadiene above 2.5 and a guaiacol content above 0.02 mg / g will result in insufficient tobacco aroma, an overly prominent smoky sensation, and an imbalance, ultimately leading to poor overall sensory experience.

[0143] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A tobacco extract composition, characterized in that, Including methylcyclopentenolone, guaiacol, neophytadiene, cephalothrix trienol, dihydroactinol, maltol, furanone, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one, and scopolamine. By mass: (methylcyclopentenolone + guaiacol) / neophadiene ranges from 0.01 to 2.5; The content of guaiacol in the tobacco extract composition is 0.003–0.02 mg / g, based on the dry matter content.

2. The tobacco extract composition according to claim 1, characterized in that, By mass: (methylcyclopentenolone + guaiacol) / neophadiene ranges from 0.02 to 2.5; And / or, the tobacco extract composition further includes at least one of nicotine, megastigmatrienone, spirovestigma, and furfuryl alcohol.

3. The tobacco extract composition according to claim 2, characterized in that, The following conditions must be met, and the content of each ingredient is based on the dry matter content of the tobacco extract composition: (1) The content of cipertrienol is ≥0.1 mg / g; optionally, the content of cipertrienol is 0.55~3.5 mg / g; (2) The content of 6-ethyl-5,6-dihydro-2H-pyran-2-one is ≥0.014 mg / g; optionally, the content of 6-ethyl-5,6-dihydro-2H-pyran-2-one is 0.015~0.3 mg / g; (3) The content of megastigmatrienone is ≥0.12 mg / g; optionally, the content of megastigmatrienone is 0.12 to 0.6 mg / g; (4) Palmitic acid content ≥ 0.011 mg / g; optionally, palmitic acid content is 0.011–0.65 mg / g; (5) Scopolamine content ≥ 0.008 mg / g; optionally, scopolamine content is 0.01~0.20 mg / g; (6) Neophytadiene content ≥ 0.012 mg / g; optionally, neophytadiene content is 0.012–0.12 mg / g; (7) The content of dihydroactinol is ≥0.01mg / g; optionally, the content of dihydroactinol is 0.01~0.055mg / g; (8) Spirophyrin content ≥ 0.01 mg / g; optionally, spirophyrin content is 0.01–0.2 mg / g; (9) The maltol content is 0.0011~0.065mg / g; (10) The furanone content is 0.008 to 0.080 mg / g; optionally, the furanone includes at least one of 2(H) furanone and 5(H) furanone; (11) The content of methylcyclopentenolone is 0.003~0.03mg / g; (12) The furfuryl alcohol content is 0.005~0.08mg / g; (13) Nicotine content ≥ 1.0 mg / g; optionally, nicotine content is 1.0 to 10.0 mg / g.

4. The tobacco extract composition according to any one of claims 1 to 3, characterized in that, It also meets at least one of the following conditions, with each content calculated based on the dry matter weight of the tobacco extract composition: A. Phenol content < 0.06 mg / g; optionally, phenol content < 0.05 mg / g; B. Hydroxyacetone content < 0.05 mg / g; Optional, hydroxyacetone content < 0.055 mg / g; C. Total nitrosamines < 0.05 mg / g; optionally, nitrosamines include N′-nitrosonornicotinine, 4-(N-methylnitroso)-1-(3-pyridyl)-1-butanone, N′-nitrosopseudoestiline, and N′-nitrosoneonicotinine; further optionally, total nitrosamines < 0.015 mg / g.

5. A method for preparing a tobacco extract composition according to any one of claims 1 to 4, characterized in that, Includes the following steps: S1: The tobacco raw material is extracted for the first time at 25-50°C to obtain the first extract. The extraction solvent used for the first extraction includes an 80wt%-95wt% aqueous ethanol solution. The first extract is then extracted for the second time at -20-0°C to obtain tobacco flavor compounds. S2: Extract tobacco raw materials at 100-200℃, separate solid and liquid to obtain extract, treat extract with porous adsorption material to obtain tobacco flavor compounds; S3: Mix the tobacco flavoring agent and the smoke flavoring agent at a mass ratio of 10:1 to 1:10 to obtain the tobacco extract composition.

6. The preparation method according to claim 5, characterized in that, In step S1, the mass ratio of tobacco raw material to the extraction medium used in the first extraction is 1:10-15, and the first extraction time is 5-24 hours. And / or, in S1, the mass ratio of the first extract to the extraction medium used in the second extraction is 1:2 to 15, and the second extraction time is 5 to 12 hours; And / or, in S2, the mass of the extraction solvent used for extraction is 60% to 80% of the tobacco raw material, and the extraction time is 1 to 6 hours; And / or, in S2, the mass of the porous adsorption material is 1.5% to 15% of the extract, and the time for treating the extract with the porous adsorption material is 2 to 6 hours.

7. The preparation method according to claim 6, characterized in that, In step S1, the extraction medium used for the second extraction includes ethanol, and also includes at least one of water and propylene glycol; optionally, the concentration of ethanol in the extraction medium used for the second extraction is 80wt% to 95wt%. And / or, in S2, the extraction method includes any one of microwave dynamic temperature field extraction, thermal reaction extraction, and dry distillation extraction; And / or, in S2, the extraction solvent includes at least one of water, ethanol, propylene glycol, and glycerol; And / or, the tobacco raw material includes at least one of tobacco leaves, tobacco stems, tobacco powder, tobacco, tobacco waste, tobacco extract, and tobacco pure oil; optionally, when the tobacco raw material is solid, the size is ≤20 mesh; And / or, the porous adsorbent material includes at least one of activated carbon, zeolite, and diatomaceous earth.

8. An aerosol generation matrix, characterized in that, The composition includes tobacco extract, and also includes at least one of organic acids, flavorings, and atomizing agents; The tobacco extract composition is the tobacco extract composition as described in any one of claims 1 to 4 or the tobacco extract composition prepared by the preparation method as described in any one of claims 5 to 7.

9. The aerosol generation matrix according to claim 8, characterized in that, It also meets at least one of the following conditions: 1) The mass of the tobacco extract composition is 1% to 50% of the total aerosol-generating matrix; 2) The mass of the atomizing agent is 50% to 99% of the total aerosol generating matrix; optionally, the atomizing agent includes at least one of propylene glycol, glycerol, water, and ethanol; 3) The organic acid accounts for 0-3% of the total aerosol-generating matrix; optionally, the organic acid includes at least one of acetic acid, lactic acid, benzoic acid, malic acid, tartaric acid, citric acid, and levulinic acid. 4) The mass of the fragrance is 0-3% of the total mass of the aerosol-generating matrix; 5) The aerosol generating matrix includes additional nicotine, and the content of the additional nicotine is 0 to 3% of the total mass of the aerosol generating matrix.

10. The use of a tobacco extract composition as described in any one of claims 1 to 4, or a tobacco extract composition prepared by any one of claims 5 to 7, or an aerosol generating matrix as described in claim 8 or 9, in an atomizing device.

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