Hydrolases TRIZ Cases

Fusing a starch binding protein tag with target enzymes creates recombinant proteins that bind to matrices.

Genetically edited dairy animals produce milk without prior pregnancy by modifying the prolactin receptor gene to eliminate hormone injection requirements.

Adaptor segments stabilize guide RNAs against nuclease degradation and tether donor polynucleotides to target sites.

PlyGRCS endolysin hydrolyzes peptidoglycan bonds to lyse resistant Staphylococcus aureus cells.

Recombinant AAV vectors flanked by transposon-derived inverted terminal repeat sequences direct genomic integration of transgenes into proliferating cells.

Cpf1 enzyme with stem-loop CRISPR RNA disrupts TCR chains to eliminate graft-versus-host disease risk in universal CAR T cell manufacturing.

A novel phospholipase C enzyme hydrolyzes major phospholipids in vegetable oils at low pH.

A foamy virus insulator fragment shields transgenes from cis-regulatory signals within retroviral vectors.

Genetically modified Acinetobacter hosts increase storage lipid concentration by knocking out competing metabolic genes.

Nick translation enzymatic system generates blunt-ended DNA fragments of predetermined sizes, eliminating reactive oxygen species damage from sonication.

Floral mosaic regulatory sequences drive editing enzymes in reproductive tissues, reducing labor costs from sequential transformations.

A split deoxyribozyme enzyme joins assembly arms to ligate mRNA substrates and enable selective protein translation.

A glycated amine measurement method uses fructosyl amino acid oxidase and protease to degrade analytes.

A metal ion-start DNA polymerase switch uses a DNAzyme catalytic module to control enzymatic activity.

MicroRNA target sites enable cell-specific Cas12a self-inactivation, reducing off-target effects during in vivo delivery.

A cell-free transcription-translation system assesses Cas nuclease variants within compartmentalized double emulsion droplets.

Amino acid substitutions increase enzyme thermostability, resolving contradictions between high-temperature sterilization and nutrient digestibility.

Engineered Aspergillus niger glucose oxidase variants reduce oxygen consumption rates while maintaining thermal stability and glucose specificity.

Administering a PTPσ inhibitor promotes nerve regeneration through CSPG-rich scars, restoring electrical homogeneity and arrhythmia resistance.

Universal calibration curve using enzyme concentration enables parallel inhibitor comparison, eliminating dedicated kits per compound.

A Cas9-crRNA complex directs site-specific double-strand breaks in target DNA through complementary base pairing.

A recombinant microorganism produces lactams by converting omega-amino acids into acyl-CoA intermediates.

A conditionally activatable siRNA sensor uses complementary nucleic acid strands to enable targeted RNA interference.

Alkaline serine endopeptidase macerates meat tissue to release intact parasites, eliminating acid handling risks while maintaining detection sensitivity.

Specific amino acid substitutions increase variant phytase thermostability and pH resistance, maintaining activity during high-temperature feed processing.

A mutant acid phosphatase catalyzes the phosphorylation of L-ascorbic acid to produce L-ascorbate-2-phosphate.

Engineered plasma cells express therapeutic proteins through precise genome editing of primary human B cells using nucleoplasmin-mediated delivery.

Recombinant microbes replace costly synthetic chemistry by using engineered enzymes to produce fatty amides with controlled chain lengths.

A microfluidic cartridge separates bacterial cells from blood using acoustic waves and detects them with recombinant bacteriophage reporter genes.

Engineered double-stranded DNA deaminases convert cytosines to uracils without requiring a denaturation step, simplifying methylation profiling workflows.

Base editing enzymes modify gamma globin promoters to reactivate fetal hemoglobin, avoiding double-strand break toxicity.

Integrated CRISPR-Cas targeting and Mu transposase insertion overcome scalability limits of conventional genome editing tools.

Site-directed mutagenesis of FAST-PETase at positions 212 and 277 increases PET degradation rates by up to 181%, addressing low industrial application value.

STEAP-1 antibody binding identifies circulating tumor cells, replacing invasive biopsies to improve diagnostic reliability and reduce unnecessary side effects.

Engineered microorganisms produce indigoid dye precursors from indole feed compounds, eliminating harsh chemical reductants and sulfate waste.

Synchronous cell disruption and enzyme activity measurement enable high-flux screening of thermophilic L-asparaginase mutants.

Zinc finger nucleases target the human beta-hemoglobin gene to introduce precise double-stranded breaks for genome correction.

Expressing lipase alongside assembly enzymes remodels triacylglycerols to increase target fatty acid concentration while maintaining total oil accumulation.

Segmenting the BCL11A enhancer into functional regions enables precise CRISPR/Cas9 cleavage to reactivate fetal hemoglobin production.

High temperature cultivation evaporates volatile compounds from fermentation broth to overcome product inhibition.

A CasY transactivating noncoding RNA stabilizes the ribonucleoprotein complex for precise genome editing.

Targeted acyltransferase activity controls medium-chain fatty acid chain lengths while maintaining high total lipid productivity.

Computational mutagenesis of the Cas9 HNH domain resolves the trade-off between high mutagenesis rates and off-target cleavage frequency.

Genetically modified Rhodococcus rhodochrous strain converts acrylonitrile to acrylic acid via nitrilase enzyme activity.

Segmented expression cassettes resolve the complexity of cloning systems while enabling precise genome editing in orphan crops.

Engineered I-CreI meganuclease variant targets glutamine synthetase DNA sequences, eliminating residual expression.

A site-specific integration system introduces transgenic target sites into plant genomes using double-strand-break agents for precise genomic positioning.

Particle bombardment delivers CRISPR components into non-epidermal plant cells, overcoming low delivery efficiency barriers.