A method of inducing sleep disorders in drosophila

Abstract

The embodiment of the present application discloses a method for inducing sleep disorders of fruit flies, comprising: placing fruit flies in a specific environment model for a preset time period, wherein the specific environment model adopts a light-dark cycle simulating sunrise and sunset in deep space and an environment simulating space density in a space station; detecting sleep conditions of the fruit flies in the specific environment model through monitoring fruit fly sleep experiments, and determining whether the fruit flies are induced to sleep disorders. The technical scheme of the embodiment of the present application creates an environment model simulating the light-dark cycle in deep space and the narrow activity space in the space station, studies sleep disorders induced by extreme environments in deep space, and thus makes it possible to deeply study the neural mechanism behind.

Claims

1. A method for inducing sleep disorders in fruit flies, characterized in that, include: Fruit flies are placed in a specific environmental model for a preset period of time. The specific environmental model adopts an environment that simulates the light and dark cycle of sunrise and sunset in deep space and the space density in a space station. The environment that simulates the space density in a space station refers to simulating the environment of fruit flies in a space station. Since the activity space in a space station is narrow, the activity space of fruit flies is compressed to simulate the environment of fruit flies in a space station. The sleep status of fruit flies in the specific environmental model was detected by monitoring fruit fly sleep experiments, and it was determined whether the fruit flies were induced to have sleep disorders. The simulated light-dark cycle of sunrise and sunset in deep space includes: By controlling the lighting, the light and dark cycle of day and night in deep space is simulated by setting a preset lighting time / preset darkness time, wherein the preset lighting time and the preset darkness time are less than 2 hours.

2. The method for inducing sleep disorders in fruit flies according to claim 1, characterized in that, The simulated space station environment includes: By compressing the fruit flies' activity space to a preset size and increasing the number of fruit flies to a preset number, the fruit flies' activity space becomes narrow during sleep monitoring.

3. The method for inducing sleep disorders in fruit flies according to claim 1, characterized in that, The method of detecting the sleep status of fruit flies in the specific environmental model by monitoring fruit fly sleep includes: The sleep patterns of fruit flies in a specific environmental model were detected by monitoring fruit fly sleep using the DAM system.

4. The method for inducing sleep disorders in fruit flies according to claim 1, characterized in that, The preset light / preset dark time cycle is a 45-minute light / 45-minute dark cycle.

5. The method for inducing sleep disorders in fruit flies according to claim 2, characterized in that, The process of compressing the fruit fly's activity space to a preset volume includes: The activity space of fruit flies is compressed to 1 / 2 of the volume of the dish / tube they are in.

6. The method for inducing sleep disorders in fruit flies according to claim 5, characterized in that, The process of compressing the fruit fly's activity space to a preset volume includes: The fruit fly contained 1 / 2 volume of food in a dish / tube.

7. The method for inducing sleep disorders in fruit flies according to claim 6, characterized in that, The food composition for fruit flies is 2% agar powder + 5% sucrose.

8. The method for inducing sleep disorders in fruit flies according to claim 1, characterized in that, The step of placing fruit flies in a specific environmental model for a continuously preset period of time includes: Fruit flies were placed in a specific environmental model for 8 days.

9. The method for inducing sleep disorders in fruit flies according to claim 1, characterized in that, The environmental conditions for the specific environmental model are: temperature 25℃, humidity 60%, and light intensity 200 Lux.

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