A pesticide insecticide containing methiprid and cyantraniliprole and its application

Abstract

The present invention discloses a pesticide insecticide containing methoprene and cyantraniliprole and its application. The pesticide insecticide includes an active component, wherein the active component is composed of methoprene and cyantraniliprole; the mass ratio of methoprene to cyantraniliprole is 1:30 to 30:1. Due to the specific selection of methoprene and cyantraniliprole and their compounding according to the ratio provided by the present invention, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a wide control spectrum, especially having good control effect on fall armyworm, cowpea thrips and American leafminer.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, in particular to a pesticide insecticide containing methoprene-amid and cyantraniliprole and applications thereof. Background Art

[0002] The fall armyworm (Spodoptera frugiperda), a member of the Noctuidae family of the order Lepidoptera, is a polyphagous agricultural pest. Characterized by strong flight, high reproduction numbers, voracious feeding, and a wide host range, the fall armyworm primarily feeds on grasses. During its larval stage, it can damage major crops such as corn, rice, sorghum, and cotton, as well as various vegetables, fruits, and ornamental plants.

[0003] Cowpea thrips is a general term for the common giant thrips (Megalurothrips usitatus Bagrall) and the flower thrips (Franeliniella intonsa Trybom). It belongs to the family Thripidae of the order Thysanoptera of the class Insecta. It is an important economic pest in agriculture and can harm a variety of crops such as cowpea, soybean and peanut. Cowpea thrips adults and nymphs use rasp-sucking mouthparts to suck the sap from the tender tissues and organs of cowpea plants, damaging stems, leaves, flowers and pods. This can cause leaf wrinkling and deformity, plant wilting, flower and pod drop, and young pod deformity, seriously affecting cowpea yield and quality. It is one of the most serious insect pests in cowpea production.

[0004] The American leafminer (Liriomyza sativae Blanchard), a member of the family Mylidae in the order Diptera, is a polyphagous pest that infests vegetables, flowers, and other plants. With a wide host range and rapid spread, the flyminer poses a significant threat to vegetable production in my country. In Guangzhou, 17 to 20 generations can occur annually, and in Luoyang, Henan, 9 to 11 generations occur, with overlapping generations. Cowpeas and other vegetables are susceptible to the flyminer, and are most severely affected. The peak season is July and August, when infestation rates can reach 100% of plants and over 80% of leaves, resulting in severe yield losses or even total crop failure. The damage caused by the flyminer to cowpeas is devastating.

[0005] Dimethoprim-amide is a pyrazole amide compound, its English common name: dimpropyridaz; trade name: Axalion TM IUPAC name: 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide; CAS registration number: 1403615-77-9; molecular formula: C 16 H 23N5O; Mefenoxam has excellent performance and good systemic conductivity. It is mainly used for field crops such as fruit trees and vegetables, soybeans, other legumes, cotton, cereals, potatoes, as well as flowers and ornamental plants. It controls pests such as Lepidoptera (striped stem borer, beet armyworm, diamondback moth, fall armyworm, etc.), Coleoptera (beetles, corn root leaf beetle, potato leaf beetle, yellow flea beetle, etc.), Diptera (flies, mosquitoes, vegetable leafminer, etc.), Hemiptera (aphids, planthoppers, psyllids, whiteflies, etc.), Thysanoptera (thrips, orchid thrips, palm thrips, tobacco thrips, etc.), Isoptera (termites, etc.), cockroaches, and ants. It is particularly effective against piercing-sucking pests such as aphids, whiteflies, and psyllids. Its structural formula is shown below:

[0006]

[0007] Cyantraniliprole, chemical name: 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)hydroxy]phenyl]-1H-pyrazole-5-carboxamide, chemical formula: C 19 H 14 BrClN6O2. Cyantraniliprole is produced by modifying various polar groups on the benzene ring. It is more effective and applicable to a wider range of crops. It can effectively control Lepidoptera, Hemiptera, and Coleoptera pests. The structural formula is shown below:

[0008]

[0009] Because corn and vegetable planting areas are wide, and the fall armyworm, cowpea thrips, and American leafminer reproduce rapidly, their prevention and control is difficult and the harm is serious. Many of the currently commonly used control agents are old agents with a long history and high resistance, and the control effect is poor. Therefore, the existing technology has compounded ingredients with different mechanisms of action, and then carried out pest control to avoid the generation of pest resistance. However, after compounding, it is necessary to judge whether this compounding is synergistic, additive, or antagonistic according to the actual application effect, but usually what is obtained is a compound formula with antagonistic effect, a compound formula with additive effect is rare, and a compound formula with very obvious synergistic effect and high co-toxicity coefficient is even rarer. Therefore, providing a compound formula with excellent preventive effect is a problem that needs to be solved urgently in the prior art. Summary of the Invention

[0010] In view of the above problems, the present invention provides a pesticide insecticide containing cyantraniliprole and anthracene, and its application, which overcomes the above problems or at least partially solves the above problems, and can solve the problem that fall armyworm, cowpea thrips and American leafminer are difficult to control in the existing technology.

[0011] Specifically, the present invention provides a pesticide insecticide containing methoprene and cyantraniliprole, including active components, wherein the active components are composed of methoprene and cyantraniliprole; the mass ratio of methoprene to cyantraniliprole is 1:30 to 30:1.

[0012] In an optional embodiment, the mass ratio of cyantraniliprole to anthracene is 1:7 to 7:1.

[0013] In an optional embodiment, the mass ratio of cyantraniliprole to anthracene is 1:1.

[0014] In an optional embodiment, the ratio of the mass of the thiamethoxam to the mass of the pesticide is 1-30%.

[0015] In an optional embodiment, the ratio of the mass of the thiamethoxam to the mass of the pesticide is 10%.

[0016] In an optional embodiment, the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 1-30%.

[0017] In an optional embodiment, the ratio of the mass of the cyantraniliprole to the mass of the pesticide is 10%.

[0018] In an optional embodiment, the pesticide is in the form of a liquid preparation.

[0019] In an optional embodiment, the pesticide further comprises adjuvants, and the adjuvants comprise surfactants, antifreeze agents, solvents and deionized water.

[0020] The surfactant includes but is not limited to nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxypropylene polyoxyethylene ether, phenethylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, anhydrous sorbitan fatty acid ester, anhydrous sorbitan One or more of fatty acid ester ethylene oxide adduct, calcium dodecylbenzenesulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, and castor oil polyoxyethylene ether;

[0021] The antifreeze agent includes but is not limited to one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropyl alcohol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, and isooctyl alcohol;

[0022] The solvent includes but is not limited to one or more of solvent oil, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oil, methyl vegetable oil, dimethylformamide, acetonitrile, polyethylene glycol, decanoic acid amide, tributyl phosphate, butyrolactone, N-long chain alkyl pyrrolidone, octyl pyrrolidone, and isooctyl alcohol.

[0023] The present invention provides use of the pesticide according to any one of the above items in controlling fall armyworm, cowpea thrips and leafminer.

[0024] The beneficial effects of the present invention are:

[0025] In the pesticide insecticide containing methoprene and cyantraniliprole provided by the present invention, since methoprene and cyantraniliprole are specifically selected and compounded according to the ratio provided by the present invention, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a wide control spectrum, especially having good control effect on fall armyworm, cowpea thrips and American leafminer.

[0026] Furthermore, when the mass ratio of methoprene to cyantraniliprole was 1:7 to 7:1, the combination showed a synergistic effect against cowpea thrips. In particular, when the mass ratio of methoprene to cyantraniliprole was 1:1, the CTC value was 167.32, showing a significant synergistic effect. DETAILED DESCRIPTION

[0027] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed.

[0028] The present invention provides a pesticide insecticide containing methoprene and cyantraniliprole, comprising an active component, wherein the active component is composed of methoprene and cyantraniliprole; the mass ratio of methoprene to cyantraniliprole is 1:30 to 30:1. Because the embodiments of the present invention specifically select methoprene and cyantraniliprole to be compounded in a ratio of 1:30 to 30:1, the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a wide control spectrum, especially having good control effect on fall armyworm, cowpea thrips and American leafminer.

[0029] It should be noted that the pesticide provided by the present invention is used to control insect pests of crops including but not limited to fall armyworm, cowpea thrips and American leafminers, but the pesticide has better control effect on fall armyworm, cowpea thrips and American leafminers.

[0030] Specifically, the mass ratio of cyantraniliprole to bromofenac is 1:30, 1:10, 1:3, 3:7, 3:5, 1:1, 5:3, 7:3, 3:1, 15:1, or 30:1. Preferably, the mass ratio of cyantraniliprole to bromofenac is 3:5, 5:3, or 1:1.

[0031] Furthermore, the pesticide insecticide further comprises adjuvants, which include surfactants, antifreeze, solvents and deionized water. The surfactants include but are not limited to nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxypropylene polyoxyethylene ether, phenethylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, anhydrous sorbitan fatty acid ester, anhydrous sorbitan One or more of fatty acid ester ethylene oxide adduct, calcium dodecylbenzenesulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, and castor oil polyoxyethylene ether;

[0032] The antifreeze agent includes but is not limited to one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropyl alcohol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, and isooctyl alcohol;

[0033] The solvent includes but is not limited to one or more of solvent oil, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oil, methyl vegetable oil, dimethylformamide, acetonitrile, polyethylene glycol, decanoic acid amide, tributyl phosphate, butyrolactone, N-long chain alkyl pyrrolidone, octyl pyrrolidone, and isooctyl alcohol.

[0034] The features and performance of the present invention are further described in detail below with reference to specific embodiments.

[0035] Example 1: 20% cyantraniliprole·methoxazole microemulsion (1:1)

[0036] 10.0 g of cyantraniliprole, 10.0 g of methamidotriazole, 10.0 g of sorbitan monooleate, 3.0 g of polyoxyethylene sorbitan monooleate, 1.5 g of castor oil polyoxyethylene ether, 2.0 g of ethylene glycol, and 7.5 g of caprylic amide were mixed and stirred into a uniform transparent oil phase, and then 56.0 g of deionized water was slowly added while stirring to form a uniform transparent liquid to obtain a pesticide insecticide.

[0037] It should be noted that the embodiments of the present invention only list microemulsions, but it is understandable that other types of liquid preparations are also within the scope of protection of the embodiments of the present invention.

[0038] Comparative Example 1: 10% cyantraniliprole microemulsion

[0039] 10.0 g of cyantraniliprole, 6.0 g of sorbitan monooleate, 2.0 g of polyoxyethylene sorbitan monooleate, 1.2 g of castor oil polyoxyethylene ether, 2.0 g of ethylene glycol, and 3.5 g of caprylic amide were mixed and stirred into a uniform transparent oil phase, and then 75.3 g of deionized water was slowly added while stirring to form a uniform transparent liquid to obtain a pesticide insecticide.

[0040] Comparative Example 2: 10% methoxazole microemulsion

[0041] Mix 10.0 g of methoxam, 5.0 g of sorbitan monooleate, 3.0 g of polyoxyethylene sorbitan monooleate, 1.0 g of castor oil polyoxyethylene ether, 2.5 g of ethylene glycol, and 4.0 g of caprylic amide and stir to form a uniform transparent oil phase. Then, slowly add 74.5 g of deionized water while stirring to form a uniform transparent liquid to obtain a pesticide insecticide.

[0042] The pesticides and insecticides provided in the above examples and comparative examples were used in the following test examples to verify the toxicity and efficacy of the pesticides and insecticides.

[0043] Test Example 1 Biological Activity Test on Fall Armyworm

[0044] This test was conducted in accordance with NYT 1154.10-2008 Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 10: Artificial Diet Mixture Method. The effects of methoprene, cyantraniliprole and their mixtures in different mass ratios on Spodoptera frugiperda were measured to determine the optimal mixing mass ratio of the two insecticides.

[0045] A. Fall armyworm (Spodoptera frugiperda) was collected from Linyi, Shandong Province, and subsequently continuously reared indoors at a temperature of 25°C ± 1°C, a humidity of 60%-70%, and a photoperiod of L:D = 16:8. No insecticide exposure was observed during this period.

[0046] B. The test agent is 90% methamidin and 94% cyantraniliprole technical

[0047] C. The mixing ratios of anithripride and cyantraniliprole are: 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3. The concentrations are shown in Table 1:

[0048] Table 1 Dosage ratio of methiprid and cyantraniliprole

[0049]

[0050]

[0051] D. Toxicity test method

[0052] The feed mixing method refers to NYT 1154.10-2008 Pesticide Indoor Bioassay Test Guidelines Insecticides Part 10: Artificial feed mixing method; first, the mother solution was diluted with acetone to 6 series gradients, and then 1 mL of the drug solution of different concentrations was dropped on the prepared artificial feed. After continuous kneading and mixing, the mixed feed was placed in a perforated culture dish and inoculated with test insects. Each treatment was set up with 4 replicates, and the experiment was set up as a blank control; each replicate had 15 3rd-instar fall armyworm larvae; 48 hours after the drug was applied, if the larvae did not respond when touched lightly with a brush or showed obvious symptoms of poisoning (deformity, twitching, cessation of eating, etc.), they were considered dead, the number of deaths was recorded, and the mortality rate and adjusted mortality rate were calculated. DPS software was used as a statistical tool to calculate the toxicity regression equation, LD 50 .

[0053]

[0054]

[0055] When the mortality rate of the blank control is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0056] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0057] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (1), formula (2), and formula (3):

[0058]

[0059] Where: ATI—measured toxicity index of mixture;

[0060] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0061] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0062] TTI=A×P A +B×P B (2)

[0063] Where: TTI—theoretical toxicity index of mixture;

[0064] A—Agent toxicity index;

[0065] P A —The percentage of agent A in the mixture, expressed as percentage (%);

[0066] B—B agent toxicity index;

[0067] P B —The percentage of agent B in the mixture, in percentage (%).

[0068]

[0069] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0070] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0071] E. Test results table 2:

[0072] Table 2 Toxicity test results of different ratios of methoprene-amid and cyantraniliprole

[0073]

[0074] According to Table 2, the effective inhibitory concentrations of the two mixtures (methipram: cyantraniliprole = 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) on the fall armyworm were 0.373 mg / L, 0.295 mg / L, 0.221 mg / L, 0.160 mg / L, 0.116 mg / L, 0.113 mg / L, and 0.112 mg / L, respectively, and the co-toxicity coefficients (CTCs) were 125.52, 133.23, 143.32, 149.57, 165.78, 152.24, and 143.51, respectively; it can be seen that the mixtures of methoprene and cyantraniliprole showed synergistic effects on the fall armyworm.

[0075] Test Example 2: Biological Activity Test on Liriomyza sativae Blanchard

[0076] This experiment was conducted with reference to the leaf dip method proposed by the International Resistance Action Committee (IRAC). The effects of methoprene, cyantraniliprole and their mixtures in different mass ratios on the leafminer Liriomyza sativae were measured to determine the optimal mixing mass ratio of the two insecticides.

[0077] A. Liriomyza sativae Blanchard was collected from cowpeas grown in the suburbs of Qingdao in September 2022. The seedlings, previously untreated, were subcultured indoors on clean bean seedlings at a temperature of 25±1°C, a relative humidity of 50%–70%, and a photoperiod of 16:8 hours (L:D). Before the experiment, the clean, potted bean seedlings were placed in a mesh enclosure with a high density of adult Liriomyza sativae for 24 hours to allow adult Liriomyza sativae to lay eggs on the seedlings. The seedlings, bearing Liriomyza sativae eggs, were then reared in a 25°C greenhouse. Testing was performed when tunnels approximately 0.5 cm long appeared on the bean leaves.

[0078] B. The test agent is 90% methamidin and 94% cyantraniliprole technical

[0079] C. The mixing ratios of anithripride and cyantraniliprole are: 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3. The concentrations are shown in Table 3:

[0080] Table 3 Dosage ratio of methiprid and cyantraniliprole

[0081]

[0082] D. Toxicity test method

[0083] Using the leaf dip method recommended by the International Resistance Action Committee (IRAC), fresh, long-stalked compound leaves infested with Liriomyza sativae larvae were collected from the middle and upper parts of beans. Leaf collection criteria: approximately 1–3 second-instar test insects per leaf, with each tract measuring 0.5–1 cm in length. The insect positions on the tracts were marked with a marker. The test pesticide was initially dissolved in methanol and then prepared with Tween-80 aqueous solution to varying concentrations. Marked leaves were dipped in the pesticide dilution for 5 seconds, then removed. The petioles were wrapped with wet cotton balls to maintain moisture, placed in insect boxes, and maintained at (25±1)°C for observation. Four replicates were performed for each treatment, with 10 test insects per replicate. Results were examined under a binocular dissecting microscope 48 hours after treatment. Larvae death criteria: larvae with shrunken and blackened bodies and no or little extension of the larval passages were considered dead, while larvae with fresh and moist bodies and naturally extended larval passages or normal eclosion were considered alive. The number of deaths was recorded, and the mortality rate and corrected mortality rate were calculated using DPS software as a statistical tool to calculate the toxicity regression equation and LD. 50 .

[0084]

[0085]

[0086] When the mortality rate of the blank control is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0087] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0088] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (4), formula (5), and formula (6):

[0089]

[0090] Where: ATI—measured toxicity index of mixture;

[0091] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0092] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0093] TTI=A×P A +B×P B(5)

[0094] Where: TTI—theoretical toxicity index of mixture;

[0095] A—Agent toxicity index;

[0096] P A —The percentage of agent A in the mixture, expressed as percentage (%);

[0097] B—B agent toxicity index;

[0098] P B —The percentage of agent B in the mixture, expressed in percentage (%).

[0099]

[0100] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0101] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0102] E. Test results table 4:

[0103] Table 4 Toxicity test results of different ratios of methoprene and cyantraniliprole

[0104]

[0105]

[0106] According to Table 4, the effective inhibitory concentrations of the seven mixtures (methipram: cyantraniliprole = 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) on cowpea leafminer were 5.67 mg / L, 5.54 mg / L, 5.36 mg / L, 6.12 mg / L, 6.83 mg / L, 7.79 mg / L, and 8.54 mg / L, respectively, and the co-toxicity coefficients (CTCs) were 138.85, 146.03, 157.46, 148.61, 144.36, 133.30, and 126.07, respectively; it can be seen that the mixtures of methoprene and cyantraniliprole all showed synergistic effects on leafminer.

[0107] Test Example 3 Activity Test on Thrips

[0108] This experiment was conducted with reference to the leaf tube film method in accordance with NY / T 3680-2020 Technical Specification for Resistance Monitoring of Western Flower Thrips. The effects of methoprene, cyantraniliprole and their mixtures at different mass ratios on cowpea thrips were determined to screen the optimal mixing mass ratio of the two insecticides.

[0109] A. Cowpea thrips (Frankliniella intonsa Trybom) were collected from cowpeas grown in the suburbs of Qingdao. They had not been exposed to any pesticides and had been reared indoors on clean green beans. Rearing conditions were a temperature of 26±1°C, a relative humidity of 50%-70%, and a photoperiod of 14h:10h. Rearing procedures involved placing two green beans in separate bottles, inserting 40 female adults, and allowing them to lay eggs. After two days, the beans were removed and placed in new bottles. Testing was performed after all second-instar nymphs had hatched.

[0110] B. The test agent is 90% methamidin and 94% cyantraniliprole technical

[0111] C. The mixing ratios of anithripride and cyantraniliprole are: 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3. The concentrations are shown in Table 5:

[0112] Table 5 Dosage ratio of methoprene and cyantraniliprole

[0113]

[0114]

[0115] D. Toxicity test method

[0116] Refer to the NY / T 3680-2020 Technical Specification for Monitoring Resistance of Western Flower Thrips - Leaf Film Method. First, dissolve the test agent directly in acetone, then dilute to the desired concentration with Tween-80 solution. Use a pipette to add the solution to a centrifuge tube. Shake the tube upside down several times, then discard the solution. Open the tube cap and place it on the bench to air dry. Then, heat the tip of an insect stinger with an alcohol lamp until it is red hot. Then, poke several small holes in the cap and bottom of a 4 cm centrifuge tube, using a diameter sufficient to prevent thrips from escaping. Then cut the fresh cowpea pods into segments of about 2 cm in length with a knife, immerse them in the test solution for 15 seconds, and then put them on absorbent paper to dry naturally. Then clamp them into a centrifuge tube, and use a sucker to suck out 15 2nd-instar cowpea thrips nymphs and put them into the centrifuge tube; then place the tube in an artificial climate box with a temperature of (26±1)℃ and a light setting of L:D=14h:10h. After 48 hours, check the death of the test insects, touch the insect body lightly with the tip of a small brush, and those that do not move are considered dead. Each tube is a replicate, and each concentration is repeated 4 times. Tween-80 aqueous solution is used as a blank control, the number of deaths is recorded, and the mortality rate and corrected mortality rate are calculated. DPS software is used as a statistical tool to calculate the toxicity regression equation, LD50 .

[0117]

[0118]

[0119] When the mortality rate of the blank control is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality rate formula. When the mortality rate is >20%, the test is repeated.

[0120] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the data were processed using DPS software to calculate the LD of each agent. 50 LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0121] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (7), formula (8), and formula (9):

[0122]

[0123] Where: ATI—measured toxicity index of mixture;

[0124] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0125] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0126] TTI=A×P A +B×P B (8)

[0127] Where: TTI—theoretical toxicity index of mixture;

[0128] A—Agent toxicity index;

[0129] P A —The percentage of agent A in the mixture, expressed as percentage (%);

[0130] B—B agent toxicity index;

[0131] P B —The percentage of agent B in the mixture, expressed in percentage (%).

[0132]

[0133] Where: CTC—co-toxicity coefficient; ATI—measured toxicity index of mixture; TTI—theoretical toxicity index of mixture.

[0134] A co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0135] E. Test results Table 6:

[0136] Table 6 Toxicity test results of different ratios of methoprene-amid and cyantraniliprole

[0137]

[0138] According to Table 6, the effective inhibitory concentrations of the seven mixtures (methipram: cyantraniliprole = 3:1, 7:3, 5:3, 1:1, 3:5, 3:7, 1:3) on cowpea thrips were 2.87 mg / L, 2.73 mg / L, 2.76 mg / L, 2.93 mg / L, 3.83 mg / L, 4.70 mg / L, and 5.21 mg / L, respectively, and the co-toxicity coefficients (CTCs) were 135.18, 148.30, 156.94, 167.32, 147.44, 132.19, and 127.78, respectively; it can be seen that the above ratios of methoprene and cyantraniliprole all showed synergistic effects on thrips.

[0139] Experimental Example 4 Field efficacy test for controlling fall armyworm

[0140] 1. Test method

[0141] Test agents: The pesticides provided in Example 1 and the pesticides provided in Comparative Examples 1 and 2 were used to control fall armyworm in the field. The test agents are shown in Table 7:

[0142] Table 7 Dosage of test drugs

[0143]

[0144] The experimental plot was located in Guojiazhuang Village, Jimo District, Qingdao City (36.437345°N, 120.5631°E). The experimental plot covers an area of 1.5 mu, with a soil pH of 6.4 and an organic matter content of 1.8%. The experimental plot belongs to the northern subtropical monsoon climate zone, with sufficient sunlight, mild climate, distinct four seasons, moderate rainfall, and flat terrain. It has good irrigation and drainage conditions and is suitable for conducting experiments.

[0145] Sowing situation: Corn seeds were purchased from the market and sown on June 18, 2022. The sowing rate per mu was 4 kg. They were sown manually, with 2 seeds per hole. The row spacing was 55 cm and the hole spacing was 25 cm. About 4,800 holes were sown per mu. After the corn seedlings were fully grown, thinning or transplanting was carried out. The management conditions were in line with local agricultural production practices.

[0146] Application time and growth period: The application time is July 28, 2022, when corn is in the seedling stage and fall armyworm is in the peak egg hatching period. The water consumption for application is 600L / hm2. 2 .

[0147] Control effectiveness survey: Insect population survey method: Sample five locations per plot, randomly survey 10 corn plants at each location, and examine the number of live insects within the corn plants. Insect populations were recorded before application and 3 and 7 days after application, and the insect population reduction rate and control efficacy were calculated.

[0148] Pharmacological efficacy calculation method

[0149] EXCEL was used to calculate the disease index and control efficacy, and the Duncan's new multiple range (DMRT) method was used to analyze the variance of the experimental data using DPS9.5 software.

[0150]

[0151]

[0152] 2. Test results

[0153] The control effects of different pesticide treatments on the lepidopteran pest Spodoptera frugiperda are shown in Table 8 below.

[0154] Table 8 Control effects of different pesticides on corn armyworm

[0155]

[0156] As shown in Table 8, the results of the control efficacy investigation show that when the dosage of the formulation is 10 g / mu, the control efficacy of Example 1 is 88.92% after 3 days of application, which is significantly better than the control efficacy of 82.47% and 85.15% of Comparative Examples 1 and 2 respectively; and the control efficacy of Example 1 is 94.22% after 3 days of application, which is significantly better than the control efficacy of 85.58% and 89.34% of Comparative Examples 1 and 2 respectively. Field observations show that both the test and control formulations are safe for the test corn crop, and no symptoms of phytotoxicity (such as stunting, chlorosis, deformity, etc.) were found.

[0157] Experimental Example 5 Field efficacy test for controlling cowpea thrips

[0158] 1. Test method

[0159] Test agents: The pesticides provided in Example 1 and the pesticides provided in Comparative Examples 1 and 2 were used to control cowpea thrips in the field. The test agents are shown in Table 9 below:

[0160] Table 9 Dosage of test drugs

[0161]

[0162] The experimental plot, located in Hanzhuang, Xiashu Town, Jurong City, Zhenjiang City, Jiangsu Province (32.178645°N, 119.167816°E), covers an area of 2 mu (approximately 1.5 acres), with a soil pH of 6.4 and an organic matter content of 1.8%. The experimental plot is located in the northern subtropical monsoon climate zone, characterized by ample sunlight, a mild climate, distinct seasons, moderate rainfall, and flat terrain, offering excellent irrigation and drainage conditions, making it suitable for the experiment.

[0163] Sowing situation: Seeds were purchased from the market and sown on May 25, 2022. The row and plant spacing was 0.25*0.4m, and the cultivation and management conditions were in line with local agricultural production practices.

[0164] Application time and growth period: The application time is August 4, 2022, when the cowpea is in the flowering stage and the thrips are in the early stage of nymphalogenesis. The water consumption for application is 600L / hm2. 2 .

[0165] Control effectiveness survey: A five-point "Z" sampling method was used within the plot, with two plants surveyed at each point, for a total of 10 plants. Two flowers were surveyed in the middle and upper parts of each plant, for a total of 40 flowers per plot. Live insect counts were recorded before application and 3 and 7 days after application. The population reduction rate and control effectiveness were calculated.

[0166] Efficacy calculation method: EXCEL was used to calculate the disease index and control efficacy, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0167]

[0168]

[0169] 2. Test results

[0170] The control effects of different pesticides on cowpea thrips are shown in Table 10 below.

[0171] Table 10 Control effect of different pesticides on cowpea thrips

[0172]

[0173] As shown in Table 10, the results of the control efficacy survey indicate that, at a dosage of 10 g / mu, Example 1 achieved a control efficacy of 87.33% after three days of application, significantly superior to the 80.54% and 85.65% control efficiencies of Comparative Examples 1 and 2, respectively. Seven days after application, Example 1 achieved a control efficacy of 90.76%, significantly superior to the 83.98% and 87.28% control efficiencies of Comparative Examples 1 and 2, respectively. Field observations indicate that both the test and control agents were safe for the test cowpea crop, with no signs of phytotoxicity (such as dwarfing, chlorosis, or deformity) observed.

[0174] Experimental Example 6 Field efficacy test for controlling Liriomyza sativae

[0175] 1 Test method

[0176] Test agents: The pesticides provided in Example 1 and the pesticides provided in Comparative Examples 1 and 2 were used to control cowpea leafminers in the field. The test agents are shown in Table 11 below:

[0177] Table 11 Dosage of each drug tested

[0178]

[0179]

[0180] The experimental plot, located in Guojiazhuang Village, Jimo District, Qingdao (36.437345°N, 120.5631°E), covers an area of 2 mu (approximately 1.5 acres), with a soil pH of 6.4 and an organic matter content of 1.8%. The experimental plot is located in the northern subtropical monsoon climate zone, characterized by ample sunlight, a mild climate, distinct seasons, moderate rainfall, and flat terrain, offering excellent irrigation and drainage conditions, making it suitable for the experiment.

[0181] Sowing situation: Seeds were purchased from the market and sown on May 10, 2022. The row and plant spacing was 0.25*0.4m, and the cultivation and management conditions were in line with local agricultural production practices.

[0182] Application time and growth period: The application time is August 12, 2022, when the cowpea is in the flowering and fruiting stage and the American leafminer is in the early stage of outbreak. The water consumption for application is 600L / hm2. 2 .

[0183] Control Effectiveness Survey: Five random points were marked in each plot, with four infested cowpeas fixed at each point, for a total of 20 infested cowpeas. A dot was marked approximately 1 cm from the front of each infested tunnel using a permanent marker, aligned with the front of the tunnel. Larvae that were fresh, plump, and had eclosion holes were counted as alive; larvae that were shrunken or discolored were counted as dead. Larvae that were difficult to identify were considered alive if they had extended tunnels, eclosion holes, or new tunnels. Insect populations were recorded before application and 3 and 7 days after application, and the population reduction rate and control efficacy were calculated.

[0184] Efficacy calculation method: EXCEL was used to calculate the disease index and control efficacy, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0185]

[0186]

[0187] 2. Test results

[0188] The control effects of different pesticides on cowpea leafminer are shown in Table 12 below.

[0189] Table 12 Control effects of different pesticides on Liriomyza sativae leafminers

[0190]

[0191]

[0192] As can be seen from the above table, the results of the control efficacy survey show that when the dosage of the formulation is 10g / mu, the control efficacy of Example 1 after 3 days of application is 87.48%, which is significantly better than the control efficacy of 80.02% and 84.38% of Comparative Examples 1 and 2 respectively. After 7 days of application, the control efficacy of Example 1 is 90.87%, which is significantly better than the control efficacy of 82.47% and 87.59% of Comparative Examples 1 and 2 respectively. Field observations show that the above test and control agents are safe for the test cowpea crop, and no symptoms of phytotoxicity (such as dwarfing, chlorosis, deformity, etc.) were found.

[0193] At this point, those skilled in the art will recognize that, although a number of exemplary embodiments of the present invention have been shown and described in detail herein, many other variations or modifications consistent with the principles of the present invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the present invention. Therefore, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.

Claims

1. A pesticide insecticide containing methoprene and cyantraniliprole for controlling fall armyworm, cowpea thrips and leafminer, characterized in that: The invention comprises active components, wherein the active components are composed of methoprene and cyantraniliprole; the mass ratio of methoprene to cyantraniliprole is 3:1 to 3:

5.

2. The use according to claim 1, characterized in that The mass ratio of the methipridyl and cyantraniliprole is 1:

1.

3. The use according to claim 1, characterized in that The ratio of the mass of the imipenem to the mass of the pesticide is 1-30%.

4. The use according to claim 3, characterized in that The ratio of the mass of the thiamethoxam to the mass of the pesticide is 10%.

5. The use according to claim 1, characterized in that The ratio of the mass of the cyantraniliprole to the mass of the pesticide is 1-30%.

6. The use according to claim 5, characterized in that The ratio of the mass of the cyantraniliprole to the mass of the pesticide is 10%.

7. The use according to claim 1, characterized in that The dosage form of the pesticide is a liquid preparation.

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