Large-scale breeding method and breeding device for aquatic fireflies

Abstract

The invention provides a breeding method and device for aquatic fireflies, and relates to the technical field of firefly breeding. The feeding device comprises a feeding frame, and a plurality of feeding boxes, a water purification tank and a water supply pipe are placed on the feeding frame. Water purification is achieved through the water purification tank, and the problem that firefly larvae are prone to death due to water deterioration is solved. According to the breeding method for the aquatic fireflies, the ampullaria gigas is used as food, artificial large-scale breeding of the aquatic fireflies is achieved, resource utilization of the ampullaria gigas is achieved, and the prevention and control pressure of the ampullaria gigas is relieved. The method is high in breeding efficiency and low in breeding cost, large-scale breeding of the aquatic fireflies is facilitated, the problems that the survival rate of the aquatic firefly larvae is low, the breeding cost is high, and the larva period is long are efficiently solved, and the problem that the aquatic firefly breeding technology does not break through large-scale breeding is successfully solved.

Description

Technical Field

[0001] The invention belongs to the technical field of firefly breeding, and in particular relates to a large-scale breeding method and a breeding device for aquatic fireflies. Background Art

[0002] Fireflies belong to the Lampyridae family of the order Coleoptera. They are holometabolous insects that go through four stages in their life cycle: egg, larva, pupa, and adult. It usually takes one year to complete a generation. The larval stage of aquatic fireflies is 9-10 months. The larvae have the behavior of gathering to attack and feed on the same prey. They live in clear, clean, slow-flowing and shallow lakes, streams or rice fields. The adult fireflies emit bright light. They are not only an important natural enemy insect resource, but also an ornamental insect resource with great development value. Due to factors such as human destruction and changes in the natural environment in recent years, the habitat of fireflies has deteriorated and the population has dropped sharply. The golden apple snail is one of the 100 most serious invasive species in the world. After being introduced into southern my country by humans, it lost its original natural enemies. The climatic conditions are very conducive to its growth and reproduction. It mainly feeds on crops such as rice seedlings, lotus roots, and wild rice stems, causing serious damage to my country's rice production. Golden apple snails may also become carriers of diseases and parasites. If they are not cooked properly or eaten raw or undercooked, they will pose a threat to human health. They will also compete with native species, leading to a reduction or even extinction of local freshwater organisms, and affecting aquatic biodiversity.

[0003] However, the meat of the golden apple snail is tender and delicious, rich in protein, carotene, multiple vitamins and minerals, and is the feed for some precious aquatic animals. Therefore, the invasive alien species headed by the golden apple snail are utilized as resources, and prevention and control are combined with utilization to turn harm into treasure. The number of golden apple snails is reduced by using aquatic firefly larvae to feed on golden apple snails, thereby reducing the harm of golden apple snails to humans and the ecological balance. As a rare landscape insect, fireflies must not only protect species but also play a role in landscape. Artificial breeding technology is the key. At present, the market value of fireflies has been highlighted. With the blessing of fireflies, many scenes have significantly increased their added value. Therefore, in response to the growing demand for fireflies, artificial breeding technology can temporarily relieve the survival threat posed by human market demand to wild firefly populations. Therefore, exploring a series of large-scale breeding devices and breeding methods for fireflies is a key issue in protecting fireflies and protecting the ecological environment. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a method for breeding aquatic fireflies, which has high breeding efficiency and low breeding cost and is suitable for large-scale breeding of aquatic fireflies.

[0005] The present invention also aims to provide a device for raising aquatic fireflies, which utilizes a water circulation system to solve the problem that firefly larvae are easily killed due to deterioration of water quality.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a device for raising aquatic fireflies. The device comprises a raising frame 1, four rollers 2 are installed at the lower end of the raising frame 1, a raising box 3 is placed on the raising frame 1, overflow ports 4 are opened on both side walls of the raising box 3, an outer end of the overflow port 4 is connected to a drainage pipe 5, a water receiving trough 6 is arranged just below the overflow port 4, and the drainage pipe 5 is connected to the water receiving trough 6; a water pipe bracket 12 is arranged on the raising frame 1, a water supply pipe 7 is installed on the water pipe bracket 12, and the water supply pipe 7 is connected to a water storage tank 8 and a clean water tank 9.

[0008] Preferably, an LED fluorescent lamp 10 is installed on the top of each layer of the breeding rack 1.

[0009] Preferably, the water supply pipe 7 is equipped with a valve 11 capable of adjusting the water volume.

[0010] Preferably, the water in the water storage tank 8 flows back to the water supply pipe 7, the water supply pipe 7 enters the breeding box 3 through the valve 11, the water in the breeding box 3 enters the drain pipe 5 through the overflow port 4, enters the clean water tank 9 through the drain pipe 5, and after purification, enters the water storage tank 8 through the submersible pump 15 to form a water circulation system.

[0011] The present invention also provides a method for raising aquatic fireflies, comprising the following steps:

[0012] Place the sponge block with firefly eggs into a plastic breeding box 3 to hatch the larvae;

[0013] Add the meat pieces of Pomacea canaliculata to the larval rearing box 3 and raise them on the rearing rack. When the larvae are 6 years old, add sand to form a gentle slope for the larvae to pupate until they become adults.

[0014] After the pupae emerge as adults, the male and female adults of the same batch are mixed and raised together. During the mixed raising process, sponge blocks are placed in the firefly adult breeding cage for the adults to lay eggs.

[0015] Collect the sponge blocks where fireflies have laid eggs, place them in a breeding box to hatch, and repeat the above breeding process.

[0016] Preferably, the incubation conditions of the eggs include a temperature of 25° C. to 28° C., an air humidity of 70% to 80%, a photoperiod of L:D=16h:8h, and a light intensity of 1000 to 1200 lx.

[0017] Preferably, the period for the eggs to hatch into larvae is 19 to 20 days.

[0018] Preferably, the mass ratio of the firefly and apple snail meat is as follows: the mass ratio of the newly hatched larvae is 200 heads: 110mg~130mg; the mass ratio of the 2nd to 3rd instar larvae is 200 heads: 180mg~200mg; the mass ratio of the 4th to 5th instar larvae is 200 heads: 410mg~430mg; the mass ratio of the 6th instar larvae preparing to pupate is 200 heads: 130mg~150mg.

[0019] Preferably, desalinated coconut husk soil is placed in the breeding cage, and the water content of the coconut husk soil is 12% to 15%.

[0020] Preferably, the method for preparing the golden apple snail meat comprises: blanching the golden apple snail in boiling water for 3 to 5 minutes, taking the golden apple snail meat out of the shell by clamping the operculum with tweezers, removing the intestinal part, and cutting into thin slices for later use.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention provides a method for large-scale breeding of aquatic fireflies using the invasive species Pomacea canaliculata as food, which not only realizes the artificial large-scale breeding of aquatic fireflies, but also utilizes the Pomacea canaliculata as a resource, thereby reducing the pressure of Pomacea canaliculata prevention and control. The method provided by the present invention has high breeding efficiency and low breeding cost, which is conducive to large-scale breeding of aquatic fireflies. The breeding method of the present invention can effectively overcome the problems of low survival rate of aquatic firefly larvae, high breeding cost, long larval stage, etc.

[0023] The breeding device of the present invention purifies water through a water purification tank, and the purified water is pumped back to the water storage tank through a submersible pump and enters the water delivery pipe, and then circulates and flows back into the breeding box, thereby making full use of water resources and solving the problem that the firefly larvae are prone to death due to water quality deterioration. The breeding device and the breeding method of the present invention are used in combination to improve the survival rate of aquatic fireflies larvae, and are suitable for large-scale breeding of all aquatic fireflies, successfully solving the problem that the aquatic firefly breeding technology has not broken through large-scale breeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention is a schematic diagram of the overall structure of the large-scale aquatic firefly breeding device; in the figure, 1 is a breeding rack, 2 is a roller, 3 is a breeding box, 4 is an overflow port, 5 is a drain pipe, 6 is a water tank, 7 is a water supply pipe, 8 is a water storage tank, 9 is a clean water tank, 10 is an LED fluorescent lamp, 11 is a valve, 12 is a water pipe bracket, 13 is a water supply tank, 14 is a light controller, and 15 is a submersible pump. DETAILED DESCRIPTION

[0025] The present invention provides a device for raising aquatic fireflies, the device comprising a raising frame 1, four rollers 2 are installed at the lower end of the raising frame 1, a raising box 3 is placed on the raising frame 1, overflow ports 4 are opened on both side walls of the raising box 3, one end of the outer side of the overflow port 4 is connected to a drain pipe 5, a water receiving trough 6 is arranged just below the overflow port 4, and the drain pipe 5 is connected to the water receiving trough 6; a water pipe bracket 12 is arranged on the raising frame 1, a water supply pipe 7 is installed on the water pipe bracket 12, and the water supply pipe 7 is connected to a water storage tank 8 and a clean water tank 9. In the present invention, the raising device comprises: a raising frame 1, four rollers 2 are installed at the lower end of the raising frame 1 for easy movement, and an LED fluorescent lamp 10 is installed on the top of each layer to supplement the light required for the growth cycle of fireflies with a time control switch. In the present invention, a plurality of raising boxes 3 are placed on the raising frame 1, overflow ports 4 are opened on both side walls of the raising box 3, and a water receiving trough 6 is arranged in the raising frame 1 and just below the overflow port 4 of the raising box 3. In the present invention, the breeding rack 1 is provided with a water pipe bracket 12, and a water supply pipe 7 is installed on the water pipe bracket 12. The water supply pipe 7 is equipped with a plurality of valves 11 for adjusting the water volume, and each valve is located directly above a breeding box 3. The water supply pipe 7 is connected with the water storage tank 8, and the water in the water storage tank 8 flows back to the water supply pipe 7. The water supply pipe 7 enters the breeding box 3 through the valve 11. The excess water in the breeding box 3 enters the drain pipe 5 through the overflow port 4, and then enters the clean water tank 9 through the drain pipe 5. The purified water enters the water storage tank 8 through the submersible pump 15. The water in the water storage tank 8 can also be pumped into the water supply tank 13 through the submersible pump 15 to form a water circulation system. Figure 1 The present invention realizes water purification through a water purification tank, and the purified water is pumped back to the water storage tank through a submersible pump and enters the water delivery pipe, and then circulates and flows back into the breeding box, thereby making full use of water resources, solving the problem that firefly larvae are prone to death due to water quality deterioration, and making the larvae have a high survival rate and a long service life, and is suitable for large-scale breeding of all aquatic fireflies.

[0026] In the present invention, the specification of the feeding rack 1 can be conventionally selected and adjusted according to actual needs. In the present invention, the specification of the feeding rack 1 is preferably length × width × height = 100-120 × 30-40 cm × 150-180 cm; more preferably 108 cm × 36 cm × 160 cm. In the present invention, the feeding rack 1 preferably has 8 layers, with a layer height of 20 cm, and a number of feeding boxes 3 are placed on each layer.

[0027] In the present invention, the specifications of the breeding box 3 can be conventionally selected and adjusted according to actual needs. In the present invention, each layer of the breeding rack 1 preferably places 4 breeding boxes 3 with specifications of length × width × height = 36cm × 25.5cm × 3.5cm. In the present invention, an overflow port 4 of 0.2 to 0.5cm is preferably opened on the side wall of the breeding box 3, and the distance between the overflow port 4 and the bottom of the breeding box 3 is preferably 0.5 to 1cm, and more preferably 0.8cm. In the present invention, a filter screen is installed inside the overflow port 4, and one end of the outside is connected to a drain pipe 5. The excess water inside the breeding box 3 can enter the drain pipe 5 through the overflow port 4, and then be discharged to the water receiving trough 6 through the drain pipe 5, and flow to the left and right sides to the bottom layer. The bottom layer of the breeding rack 1 is used as a water tank, which is connected to a clean water tank 9. Volcanic rocks, activated carbon and filter cotton are preferably placed in the clean water tank 9 to filter and clean the water. A circulating water system is placed on the far right. New water is added to the water tank through a submersible pump 15 depending on the amount of stored water to keep the water level in the water tank balanced, which can save water resources and can be reused.

[0028] In the present invention, an 8-watt LED fluorescent lamp 10 is installed on the top of each layer of the breeding rack 1. The LED fluorescent lamp 10 is controlled by a light controller 14 to supplement the light required for the growth cycle of fireflies. With the change of seasons, 4 hours are supplemented in spring and autumn, 2 hours in summer, and 6 hours in winter.

[0029] In the present invention, a water supply pipe 7 is installed 8 to 12 cm above the breeding box 3, and the water supply pipe 7 can be supported by a water pipe bracket 12. The water supply pipe 7 has a valve 11 to adjust the water output. In the present invention, the water inlet of the water supply pipe 7 can be conventionally adjusted according to actual needs. In the present invention, the water inlet is preferably 10 to 20 mL / min, more preferably 15 mL / min.

[0030] In the present invention, each layer of the breeding rack 1 is installed with an LED fluorescent lamp 10, a water pipe bracket 12, a water supply pipe 7, an adjustable water valve 11, and a water receiving tank 6.

[0031] The present invention also provides a method for raising aquatic fireflies, which includes egg hatching management, larvae and pupae feeding management, and adult to egg, and more preferably includes the following steps:

[0032] Place the sponge block with firefly eggs into a plastic breeding box 3 to hatch the larvae;

[0033] Add the meat pieces of Pomacea canaliculata to the larval rearing box 3 and raise them on the rearing rack. When the larvae are 6 years old, add sand to form a gentle slope for the larvae to pupate until they become adults.

[0034] After the pupae emerge as adults, the male and female adults of the same batch are mixed and raised together. During the mixed raising process, sponge blocks are placed in the firefly adult breeding cage for the adults to lay eggs.

[0035] Collect the sponge blocks where fireflies have laid eggs, place them in a breeding box to hatch, and repeat the above breeding process.

[0036] In the present invention, the specific steps of hatching management of the eggs are preferably as follows: the sponge block with firefly eggs is placed in a plastic breeding box padded with pebbles and then placed in a light incubator with a temperature of 25°C to 28°C, an air humidity of 70% to 80%, a light cycle of 16h during the day (L): 8h at night (D), and a light intensity of 1000 to 1200lx for hatching; the hatching breeding box is food grade, and the specifications of the breeding box are preferably length × width × height = 36cm × 25.5cm × 3.5cm. In the present invention, water with a depth of about 0.8cm to 1.2cm is preferably added to the breeding box, flat and smooth pebbles are placed in the water, and the sponge block is placed on the pebbles for hatching, and the sponge block is observed every day and sprayed with water to maintain humidity. The eggs that are about to hatch larvae will turn black. In the present invention, after the eggs are hatched for 20 to 24 hours, the sponge block is removed and placed in another breeding box of the same specification for continued hatching, which can keep the development of the firefly larvae consistent. In the present invention, the entire egg period is preferably 19 to 20 days.

[0037] In the present invention, the specific steps of feeding and managing the larvae and pupae are preferably as follows: put the golden apple snail into boiling water for 3 to 5 minutes, then use tweezers to clamp the operculum to take out the golden apple snail meat from the shell, remove the intestinal part, and cut into thin slices for later use. Add the cut golden apple snail meat pieces into the firefly larvae feeding box, and the ratio of the fireflies and snail meat is preferably as follows: the mass ratio of the number of newly hatched larvae to the snail meat is 200 heads: 110mg to 130mg; the mass ratio of the number of 2 to 3 instar larvae to the snail meat is 200 heads: 180mg to 200mg; the mass ratio of the number of 4 to 5 instar larvae to the snail meat is: 200 heads / 410mg to 430mg; the food intake of the 6th instar larvae is reduced when preparing for pupation, and the mass ratio of the number of 6th instar larvae to the snail meat is: 200 heads / 130mg to 150mg.

[0038] In the present invention, the conditions for the feeding and management of the larvae and pupae preferably include feeding on a feeding rack at a temperature of 26°C to 28°C, a photoperiod of 16:8 (L:D), and a light intensity of 1000 to 1200 lx. The aquatic fireflies are fed in a feeding box on the feeding rack for 50 to 60 days to become mature larvae. At the age of 6, a 3 cm high sandy soil is placed into a gentle slope for pupation until adulthood, and the soil slope occupies about 2 / 3 of the volume of the feeding box. Check the feeding box every day, clean the debris, check the water circulation system of the feeding device, check whether the water inlet valve is normal, feed fresh Pomacea canaliculata meat, and remove the uneaten snail meat to avoid polluting the water quality and keep the water clean.

[0039] In the present invention, the specific steps of the transformation from adult to egg are preferably as follows: after the pupae emerge into adults, the male and female adults of the same batch are mixed and raised, and after the adults emerge, they are collected and placed in plastic adult cages for feeding and management. The specifications of the plastic adult cages are preferably length × width × height: 18-20 cm × 9-10 cm × 5-7 cm. In the present invention, each of the adult cages is preferably filled with 40-60 pairs of adult fireflies, and more preferably 50 pairs of adult fireflies. In the present invention, desalinated coconut husk soil is placed at the bottom of the cage of the adult cage, and the moisture content of the coconut husk soil is preferably maintained at 12% to 15%, and more preferably 13 to 14%. In the present invention, honey cotton balls or fresh fruit pieces with a concentration of 8% to 10% are also preferably added to the cage to supplement nutrition for the adult fireflies. In the present invention, after the adults in the breeding cage begin to lay eggs, a moist sponge block is placed for them to lay eggs. The moist sponge block is replaced every day. The sponge block cutting standard is preferably 6-8cm in length×width×thickness×2.5cm-3cm. During the breeding process, the sponge blocks are placed in the firefly adult breeding cage in sequence. The sponge blocks where the fireflies have laid eggs are collected and placed in the breeding box for hatching. Finally, the collection date is marked (marked on the front of the plastic plate) to facilitate the hatching management of the eggs. In the present invention, the conditions for the adult management are preferably completed in a light incubator at 26°C-28°C, a light cycle of 16:8 (L:D), and a light intensity of 1000-1200lx.

[0040] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0041] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are commercially available products unless otherwise specified.

[0042] Example 1

[0043] The eggs of the aquatic firefly Firefly introduced from other places are placed indoors until the larvae hatch

[0044] (1) Egg incubation management: Place the sponge block containing firefly eggs in a plastic breeding box padded with pebbles and then incubate in a light incubator with a temperature of 25°C, an air humidity of 80%, a photoperiod of 16 h during the day and 8 h at night, and a light intensity of 1200 lx; ​​the hatching breeding box is of food grade specification: length × width × height = 36 cm × 25.5 cm × 3.5 cm. Add water about 0.8-1.2 cm deep into the white breeding box, put smooth pebbles in the water, and place the sponge block on the pebbles for incubation. Observe and spray the sponge block with water every day to maintain humidity. The eggs that are about to hatch will turn black. In order to maintain the uniform development of the firefly larvae, remove the sponge block after 24 hours and place it in another breeding box of the same specification for continued incubation. The entire egg period is 20 days.

[0045] (2) Larvae and pupae feeding and management: Preparation of feeding food: Put the snails into boiling water for 3-5 minutes, then use tweezers to hold the operculum to remove the snail meat from the shell, remove the intestinal part, and cut into thin slices for later use. First, add 0.8cm to 1.2cm of shallow water to the feeding box, and put 6-8 smooth pebbles at the same time. Pick 200 larvae that have hatched and put them into the feeding box for 3 times, for a total of 600 larvae. Add the cut snail meat to the firefly larvae feeding box. The ratio of firefly to snail meat for newly hatched larvae is about 200 larvae / 120mg; 2nd and 3rd instar larvae are about 200 larvae / 200mg; 4th and 5th instar larvae are about 200 larvae / 410mg; 6th instar larvae start to prepare for pupation and the food intake is reduced to about 200 larvae / 150mg. Check every day, raise them on a breeding rack with a temperature of 26℃, a photoperiod of 16:8 (L:D), and a light intensity of 1200lx. After 55 days of breeding in the breeding box on this breeding rack, the aquatic fireflies become mature larvae. At the 6th instar, put into a gentle slope of 3cm high sand for them to pupate until they become adults. The soil slope occupies about 2 / 3 of the volume of the breeding box. Check the breeding box every day, clean the debris, check the water circulation system, check whether the water inlet valve is normal, feed fresh golden apple snail meat, and remove the uneaten snail meat to avoid polluting the water quality and keep the water clean.

[0046] (3) From adult to egg: After the pupae emerge into adults, the male and female adults of the same batch are mixed and raised. After the adults emerge, they are collected and placed in plastic adult cages with a length × width × height of 20 cm × 10 cm × 7 cm for feeding and management. 50 pairs of firefly adults are placed in each cage. Desalted coconut husk soil is placed at the bottom of the cage to keep the water content of the coconut husk soil at about 15%. 10% honey cotton balls or fresh fruit pieces are added to supplement the nutrition of the fireflies. After the adults in the breeding cage begin to lay eggs, moist sponges are placed for them to lay eggs. The moist sponges are replaced every day. The sponge blocks are cut in the standard length × width × thickness of 8 cm × 5 cm × 3 cm. During the breeding process, the sponges are placed in the firefly adult breeding cage in sequence. Collect the sponge blocks where fireflies have laid eggs and place them in a breeding box for incubation. The specifications of the breeding box are: length × width × height = 36cm × 25.5cm × 3.5, and finally mark the collection date (marked on the front of the plastic plate) to facilitate the incubation management of the eggs. The process is completed in a light incubator with a temperature of 26°C, a light cycle of 16:8 (L:D), and a light intensity of 1200lx.

[0047] Example 2

[0048] The eggs of the aquatic firefly Firefly introduced from other places are placed indoors until the larvae hatch

[0049] (1) Egg hatching management: Place the sponge block containing firefly eggs in a plastic breeding box padded with pebbles and then incubate in a light incubator with a temperature of 26°C, an air humidity of 75%, a photoperiod of 16 h during the day and 8 h at night, and a light intensity of 1200 lx; ​​the hatching breeding box is of food grade specification: length × width × height = 36 cm × 25.5 cm × 3.5 cm. Add water about 0.8-1.2 cm deep into the white breeding box, put smooth pebbles in the water, and place the sponge block on the pebbles for hatching. Observe and spray the sponge block with water every day to maintain humidity. The eggs that are about to hatch will turn black. In order to maintain the uniform development of the firefly larvae, remove the sponge block after 24 hours and place it in another breeding box of the same specification for continued hatching. The entire egg period is 20 days.

[0050] (2) Larvae and pupae feeding and management: Preparation of feeding food: Put the golden apple snail into boiling water for 3-5 minutes, then use tweezers to hold the operculum to remove the golden apple snail meat from the shell, remove the intestinal part, and cut into thin slices for later use. First, add about 0.8cm-1.2cm of shallow water to the feeding box, and put 6-8 smooth pebbles at the same time. Pick 180 hatched larvae and put them into the feeding box for 3 times, a total of 540 larvae. Add the cut golden apple snail meat to the firefly larvae feeding box. The ratio of firefly to snail meat for newly hatched larvae is about 200 larvae / 120mg; 2nd and 3rd instar larvae are about 200 larvae / 200mg; 4th and 5th instar larvae are about 200 larvae / 410mg; 6th instar larvae start to prepare for pupation and the food intake is reduced to about 200 larvae / 150mg. Check every day, raise them on a breeding rack with a temperature of 27℃, a light cycle of 16:8 (L:D), and a light intensity of 1200lx. After 50 days of breeding in the breeding box on this breeding rack, the aquatic fireflies become mature larvae. At the 6th instar, put into a gentle slope of 3cm high sand for them to pupate until they become adults. The soil slope occupies about 2 / 3 of the volume of the breeding box. Check the breeding box every day, clean the debris, check the water circulation system, check whether the water inlet valve is normal, feed fresh golden apple snail meat, and remove the uneaten snail meat to avoid polluting the water quality and keep the water clean.

[0051] (3) From adult to egg: After the pupae emerge, the male and female adults of the same batch are mixed and raised. After the adults emerge, they are collected and placed in a plastic adult cage with a length × width × height of 20 cm × 10 cm × 7 cm for feeding and management. 50 pairs of fireflies are placed in each cage. Desalted coconut husk soil is placed at the bottom of the cage to keep the water content of the coconut husk soil at 12%. 10% honey cotton balls or fresh fruit pieces are added to supplement the nutrition of the fireflies. After the adults in the cage begin to lay eggs, moist sponges are placed for them to lay eggs. The moist sponges are replaced every day. The sponge blocks are cut in the standard of length × width × thickness of 8 cm × 5 cm × 3 cm. During the feeding process, the sponges are placed in the firefly adult breeding cage in sequence. The sponge blocks where the fireflies have laid eggs are collected and placed in a breeding box for incubation. The specifications of the breeding box are: length × width × height = 36 cm × 25.5 cm × 3.5. Finally, the collection date is marked (marked on the front of the plastic tray) to facilitate the hatching management of the eggs. The process was completed in a light incubator with a temperature of 26°C, a photoperiod of 16:8 (L:D), and a light intensity of 1200lx.

[0052] Example 3

[0053] The eggs of the aquatic firefly Firefly introduced from other places are placed indoors until the larvae hatch

[0054] (1) Egg incubation management: Place the sponge block containing firefly eggs in a plastic breeding box padded with pebbles and then incubate in a light incubator with a temperature of 28°C, an air humidity of 70%, a photoperiod of 16 h during the day and 8 h at night, and a light intensity of 1200 lx; ​​the breeding box for incubation is of food grade: length × width × height = 36 cm × 25.5 cm × 3.5 cm. Add water about 0.8-1.2 cm deep into the white breeding box, put smooth pebbles in the water, and place the sponge block on the pebbles for incubation. Observe and spray the sponge block with water every day to maintain humidity. The eggs that are about to hatch will turn black. In order to maintain the uniform development of the firefly larvae, remove the sponge block after 24 hours and place it in another breeding box of the same specification for continued incubation. The entire egg period is 19 days.

[0055] (2) Larvae and pupae feeding and management: Preparation of feeding food: Put the snails into boiling water for 3-5 minutes, then use tweezers to hold the operculum to remove the snail meat from the shell, remove the intestinal part, and cut into thin slices for later use. First, add about 0.8cm-1.2cm of shallow water to the feeding box, and put 6-8 smooth pebbles at the same time. Pick 160 hatched larvae and put them into the feeding box for 3 times, a total of 480 larvae. Add the cut snail meat to the firefly larvae feeding box. The ratio of firefly to snail meat for newly hatched larvae is about 200 larvae / 120mg; 2nd and 3rd instar larvae are about 200 larvae / 200mg; 4th and 5th instar larvae are about 200 larvae / 410mg; 6th instar larvae start to prepare for pupation and the food intake is reduced to about 200 larvae / 150mg. Check every day, raise them on a breeding rack at 26℃, with a light cycle of 16:8 (L:D) and a light intensity of 1200lx. After 60 days of breeding in the breeding box on this breeding rack, the aquatic fireflies become mature larvae. At the age of 6, a 3cm high sand slope is placed to form a gentle slope for them to pupate until they become adults. The soil slope occupies about 2 / 3 of the volume of the breeding box. Check the breeding box every day, clean the debris, check the water circulation system, and check whether the water inlet valve is normal. Feed fresh golden apple snail meat, and remove the uneaten snail meat to avoid polluting the water quality and keep the water clean.

[0056] (3) From adult to egg: After the pupae emerge into adults, the male and female adults of the same batch are mixed and raised. After the adults emerge, they are collected and placed in plastic adult cages with a length × width × height of 20 cm × 10 cm × 7 cm for feeding and management. 50 pairs of fireflies are placed in each cage. Desalted coconut husk soil is placed at the bottom of the cage to maintain the moisture content of the coconut husk soil at 14%. 10% honey cotton balls or fresh fruit pieces are added to supplement the nutrition of the fireflies. After the adults in the cage begin to lay eggs, moist sponges are placed for them to lay eggs. The moist sponges are replaced every day. The sponge blocks are cut in the standard length × width × thickness of 8 cm × 5 cm × 3 cm. During the feeding process, the sponges are placed in the firefly adult breeding cage in sequence. The sponge blocks where the fireflies have laid eggs are collected and placed in a breeding box for incubation. The specifications of the breeding box are: length × width × height = 36 cm × 25.5 cm × 3.5. Finally, the collection date is marked (marked on the front of the plastic tray) to facilitate the hatching management of the eggs. The process was completed in a light incubator with a temperature of 26°C, a photoperiod of 16:8 (L:D), and a light intensity of 1200lx.

[0057] Comparative Example 1

[0058] Eggs of the aquatic firefly Firefly introduced from other places.

[0059] Firefly larvae of the same size were selected and placed in a breeding box, with an inoculation amount of 180 per plate, and placed in a breeding rack at a temperature of 26°C to 28°C, an air humidity of 70% to 80%, and a photoperiod of 16h during the day and 8h at night. The fireflies were fed with large snail meat cut into the same size every day, and the survival rate, larval stage, pupation rate, and eclosion rate of aquatic fireflies were recorded. The large snails were purchased online from large snails in Weishan County, Jining City, Shandong Province, and were larger in size.

[0060] Comparative Example 2:

[0061] Eggs of the aquatic firefly Firefly introduced from other places.

[0062] Firefly larvae of the same size were selected and placed in a breeding box, with an inoculation amount of 180 per plate, and placed in a breeding rack at a temperature of 26°C to 28°C, an air humidity of 70% to 80%, and a photoperiod of 16h during the day and 8h at night, and fed with snail meat cut into the same size every day, and the survival rate, larval stage, pupation rate and eclosion rate of aquatic fireflies were recorded. The snails were collected from the Xiaosha River stream in Jinjiang District, Chengdu, and were small in size.

[0063] Under the same breeding technology and breeding device conditions, Examples 1 to 3 selected the same batch of firefly larvae that were just hatched, and three replicates were set at different stocking densities of 200, 180, and 160. Large snail meat and field snail meat were used as Comparative Examples 1 and 2, and the stocking density was 180 heads, and three replicates were set. The survival rate, larval stage, pupation rate, and eclosion rate of fireflies were observed and counted every day. Finally, the four indicators of larval survival rate, development period, pupation rate, and eclosion rate were used as standards to evaluate the feeding effects of Examples 1 to 3 and Comparative Examples 1 to 2.

[0064] Table 1 Effect of raising fireflies in Examples 1-3 and Comparative Examples 1-2

[0065] experiment Survival rate (%) Larval stage (d) Pupation rate (%) Eclosion rate (%) Example 1 70.56 58.86 70.21 68.24 Example 2 85.90 55.15 75.37 70.35 Example 3 85.69 54.94 75.41 69.89 Comparative Example 1 30.48 70.22 45.28 55.28 Comparative Example 2 85.15 56.36 75.34 69.98

[0066] According to the results described in Table 1, the average survival rate of the embodiments 1-3 of the present invention on the large-scale breeding device and breeding method for aquatic fireflies is 80.72%, the average larval stage is 56.32d, the average pupation rate is 73.66%, and the average emergence rate is 69.49%. The larval survival rate, larval development period, pupation rate and emergence rate of comparative examples 1 and 2 are 30.48% and 85.15%, 70.22d and 56.36d, 45.28% and 75.34%, 55.28% and 69.98%, respectively. The survival rate, larval development period, pupation rate and emergence rate are significantly higher than those of comparative example 1, and the breeding results of the local species of snails preferred by the aquatic fireflies, Firefly Lei's, are similar to those of comparative example 2, among which the best breeding result of embodiment 2 is equivalent to the breeding result of comparative example 2 with the same density. The cost of raising aquatic fireflies with golden apple snails is only 1 / 10 of that of raising aquatic fireflies with field snails. As an alien invasive species, golden apple snails seriously affect the production of crops such as rice and may become a carrier of diseases and parasites. Improper cooking methods, eating raw or undercooked food will threaten human health. At the same time, they compete with local species, causing a reduction or even extinction of local freshwater organisms, affecting aquatic biodiversity. Therefore, the present invention focuses on using golden apple snails as the main food source for large-scale breeding of aquatic fireflies, which not only reduces costs and reduces the harm of golden apple snails to crops such as rice, but also better protects biodiversity.

[0067] The present invention relates to a large-scale aquatic firefly breeding device and breeding method. The breeding conditions include temperature, humidity, and light cycle. The appropriate breeding time is grasped, the breeding cycle is short, and the scientific breeding method is combined to reduce labor costs and food costs, improve the survival rate, pupation rate, and eclosion rate of aquatic fireflies, and reduce the development period of firefly larvae. In exploring the breeding method of aquatic fireflies, the original problem of high breeding cost and difficulty in large-scale breeding of aquatic fireflies is gradually broken, thereby realizing large-scale breeding of aquatic fireflies.

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A device for raising aquatic fireflies, comprising a raising frame (1), wherein four rollers (2) are mounted at the lower end of the raising frame (1). It is characterized in that A feeding box (3) is placed on the feeding frame (1), and overflow ports (4) are provided on both side walls of the feeding box (3), one end of the overflow port (4) is connected to a drainage pipe (5), a water receiving trough (6) is provided just below the overflow port (4), and the drainage pipe (5) is connected to the water receiving trough (6); a water pipe bracket (12) is provided on the feeding frame (1), and a water supply pipe (7) is installed on the water pipe bracket (12), and the water supply pipe (7) is connected to a water storage tank (8) and a clean water tank (9).

2. The breeding device according to claim 1, It is characterized in that An LED fluorescent lamp (10) is installed on the top of each layer of the breeding rack (1).

3. The breeding device according to claim 1, It is characterized in that The water supply pipe (7) is equipped with a valve (11) capable of adjusting the water volume.

4. The breeding device according to claim 1, It is characterized in that The water in the water storage tank (8) flows back to the water supply pipe (7), and the water supply pipe (7) enters the breeding box (3) through the valve (11). The water in the breeding box (3) enters the drain pipe (5) through the overflow port (4), enters the clean water tank (9) through the drain pipe (5), and enters the water storage tank (8) through the submersible pump (15) after purification, thus forming a water circulation system.

5. A method for raising aquatic fireflies, It is characterized in that The steps include: Place the sponge block containing firefly eggs into a plastic breeding box (3) to hatch the larvae; Add the meat pieces of Pomacea canaliculata to the larval rearing box (3) and raise the larvae on the rearing rack. When the larvae are 6 years old, add sand to form a gentle slope for the larvae to pupate until they become adults. After the pupae emerge as adults, the male and female adults of the same batch are mixed and raised together. During the mixed raising process, sponge blocks are placed in the firefly adult breeding cage for the adults to lay eggs. Collect the sponge blocks where fireflies have laid eggs, place them in a breeding box to hatch, and repeat the above breeding process.

6. The breeding method according to claim 5, It is characterized in that The incubation conditions of the eggs include a temperature of 25°C to 28°C, an air humidity of 70% to 80%, a light cycle of L:D=16h:8h, and a light intensity of 1000 to 1200lx.

7. The breeding method according to claim 5, It is characterized in that The period for the eggs to hatch into larvae is 19 to 20 days.

8. The breeding method according to claim 5, It is characterized in that The head-to-head mass ratios of the firefly and apple snail meat are as follows: the head-to-head mass ratio of the newly hatched larvae is 200 heads: 110mg-130mg; the head-to-head mass ratio of the 2nd to 3rd instar larvae is 200 heads: 180mg-200mg; the head-to-head mass ratio of the 4th to 5th instar larvae is 200 heads: 410mg-430mg; the head-to-head mass ratio of the 6th instar larvae starting to prepare for pupation is 200 heads: 130mg-150mg.

9. The breeding method according to claim 5, It is characterized in that Desalinated coconut husk soil is placed in the breeding cage, and the water content of the coconut husk soil is 12% to 15%.

10. The breeding method according to claim 5, It is characterized in that The method for preparing the golden apple snail meat comprises: scalding the golden apple snail in boiling water for 3 to 5 minutes, taking the golden apple snail meat out of the shell by clamping the operculum with tweezers, removing the intestinal part, and cutting into thin slices for later use.

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