Method for evaluating hangover degree after drinking and application

Abstract

The invention relates to the technical field of detection, and particularly provides a method for evaluating the hangover degree after drinking and application of the method. The method comprises the following steps: respectively determining the autonomous activity change conditions of a mouse at different time points after the mouse is subjected to intragastric administration of wine samples with different hangover degrees and the same alcohol intake; by comparing and observing the increase and decrease conditions of autonomous activities after drinking of different wines, a hangover degree evaluation method capable of simultaneously detecting a time period from an abnormal excitement state to a complete waking state after drinking of excessive but non-drunkenness dosage is established, and the difference of the hangover degrees in uncomfortable symptoms after drinking of different wines can be comprehensively compared and evaluated. The method can be applied to product screening and research for improving drinking comfort.

Description

Technical Field

[0001] The invention relates to the field of detection technology, in particular to a method for evaluating the degree of hangover after drinking. Background Art

[0002] With the development of the national economy and the continuous improvement of people's living standards, people's demand for drinking is not limited to taste, but also pays attention to the physical and psychological comfort after drinking. After drinking, uncomfortable symptoms often occur, such as hangover, which usually manifests as headache, dizziness, fatigue, etc., affecting daily life. In order to alleviate these uncomfortable symptoms after drinking, a wide range of hangover products have been developed, including products that can be taken before drinking, after drinking, and can be directly mixed with wine. Different evaluation or testing methods are usually used for the improvement effect of different products on the uncomfortable symptoms after drinking. For example, the current evaluation method of the degree of hangover after drinking mainly relies on population testing, and animal experiments are more about investigating the behavioral changes of drowsiness from complete drunkenness (disappearance of righting) to sober time (righting recovery) after drinking, or changes in ethanol content, to comprehensively evaluate the effect of wine or hangover products. Some evaluation methods evaluate the severity of hangover by measuring the content of biochemical indicators or behavioral changes in the body after alcohol metabolism tends to zero after drinking.

[0003] All of the above evaluation methods are beyond the conventional dose of complete drunkenness, which will lead to obvious inhibition of the behavior of the individual being tested and make it impossible to observe behavioral changes related to hangover after drinking. Patent CN117643451A "A method for evaluating the hangover effect after drinking liquor" discloses a method for evaluating the hangover effect after drinking liquor, which mainly monitors multiple behavioral parameters of animals in a sober state after drinking, including the increase and decrease of pain threshold, sucrose preference, footprint symmetry and ataxia coefficient to evaluate the degree of hangover after drinking; Patent CN117147814A "A method for comprehensive evaluation of comfort after drinking liquor at the animal level" discloses a method for comprehensive evaluation of comfort after drinking liquor at the animal level, which mainly uses the enzyme activity of acetaldehyde dehydrogenase in the cerebellum tissue of hangover mice, the serotonin content in the hippocampus, the number of microglia in brain tissue, the FGF21 protein content in serum, the enzyme activity of catalase in liver tissue, the content of malondialdehyde in liver tissue, the content of TNF-α in liver tissue, the enzyme activity of alanine aminotransferase in liver tissue, and the enzyme activity of acetaldehyde dehydrogenase in liver tissue as indicators to comprehensively evaluate the comfort after drinking. These evaluation methods all need to be performed under complete intoxication and are not suitable for evaluating abnormal excitement after drinking excessive but intoxicating doses.

[0004] It is known that different qualities of alcoholic beverages have different effects on the body's comfort after drinking. Existing hangover remedies mostly reduce hangover symptoms by increasing the alcohol metabolism rate. However, most evaluation methods can only be performed in a state of complete drunkenness. It is difficult to observe hangover symptoms in a state of not being completely drunk, which limits the comprehensiveness of hangover evaluation.

[0005] In view of this, proposing a method for evaluating the degree of hangover after drinking wine has become one of the technical problems that need to be solved urgently. Summary of the invention

[0006] In order to solve the deficiencies in the prior art, the present invention provides a method for evaluating the degree of hangover after drinking.

[0007] The technical solution of the present invention is achieved as follows: The present invention provides a method for evaluating the degree of hangover after drinking, comprising the following steps:

[0008] Step 1: Divide the test mice into an experimental group and a control group. The mice in the control group are gavaged with alcohol once, and the mice in the experimental group are gavaged with different alcoholic products once. The total amount of alcohol in the alcoholic products gavaged once by the mice in the control group and the mice in the experimental group is the same;

[0009] Step 2: At different time points, observe the changes in the autonomic activity of the mice in the control group and the experimental group, and score the autonomic activity. According to the score, evaluate the degree of hangover of the corresponding alcohol consumed by the mice.

[0010] In some embodiments, the single intragastric volume is 0.06-0.15 ml / 10 g·bw.

[0011] In some embodiments, the different time points in step 2 are different time points within 0.5-8 hours after a single intragastric administration.

[0012] In some embodiments, in step 2, the autonomous activity scoring method includes: respectively counting the corresponding activity distances of the mice within a single activity duration starting from the corresponding time points at three different time points T1, T2 and T3, respectively recording them as D1, D2 and D3, calculating the sum of D1, D2 and D3, taking the sum of D1, D2 and D3 of the control group as 100%, respectively calculating the percentages of different test groups relative to the control group, and evaluating the degree of hangover of the wine according to the percentage values ​​of the test groups relative to the control group.

[0013] In some embodiments, the method for evaluating a specific hangover degree includes, when the percentage value of the test group relative to the control group is ≤90%, the wine is regarded as the wine with reduced hangover degree after drinking; when the percentage value of the test group relative to the control group is ≥110%, the wine is regarded as the wine with aggravated hangover degree after drinking; when the percentage value of the test group relative to the control group is >90% and <110%, the wine is regarded as the wine with no significant difference in hangover degree after drinking.

[0014] In some embodiments, step 2 further comprises detecting the blood ethanol content of the mouse at different time points.

[0015] In some embodiments, the T1 time point is the time point corresponding to the highest point of the mouse blood ethanol content, the T2 time point is the time point corresponding to the 60-70% of the highest point of the mouse blood ethanol content, and the T3 time point is the time point corresponding to the 20-30% of the highest point of the mouse blood ethanol content.

[0016] In a second aspect, the present invention also provides an application of the above method for evaluating the degree of hangover after drinking, including a method for screening products for improving the comfort of drinking.

[0017] In a third aspect, the present invention also provides an application of the above method for evaluating the degree of hangover after drinking, including a product for studying to improve the comfort of drinking.

[0018] A large number of studies have shown that lack of movement, anxiety-like symptoms and reduced activity can be observed in the hangover state. This movement disorder is related to changes in energy in the cerebral cortex. The above changes in different aspects of hangover in rodents after drinking can be evaluated by using behavioral changes. The hangover evaluation method is to use open field experiment related instruments and platforms, combined with the animal's fear of open places and avoidance of activities, and the behavioral characteristics of the tendency to new environments, to simulate the changes in human behavior and activities after drinking. By monitoring the behavioral changes such as autonomous activity and exploratory activity of animals after drinking, the correlation between behavioral changes and phenomena such as emotions and states is then analyzed. For example, the anxiety behavior measured by the time the rodent spends in the central area and the number of times it enters the central area specifically reflects the total distance of the animal's activities in different activity areas. Studies have shown that the total distance of movement activities in different areas has been significantly reduced, indicating that the exploration ability and adaptability of animals in the face of new environments after taking the test substance are significantly affected.

[0019] At present, there are many kinds of hangover products on the market, and the main mechanisms of action include reducing alcohol absorption, reducing gastric mucosal damage, accelerating alcohol metabolism, etc., but they cannot meet the needs of consumers to evaluate the discomfort symptoms that last for a long time from the abnormal excitement state to the fully sober state after drinking excessive but not drunken doses (i.e., the different time points when the serum ethanol content gradually tends from the peak value to 10% of the drunken peak value). Therefore, the difficulty of the conventional evaluation method under the dose of complete drunkenness, which causes the obvious inhibition of animal behavior and the inability to observe the behavioral changes under the condition of the discomfort symptoms related to the hangover after drinking, is overcome, and the evaluation of the degree of hangover after drinking from the abnormal excitement state to the fully sober state after drinking excessive but not drunken doses is supplemented, and the evaluation system of the comfort after drinking alcohol is broadened.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The evaluation method provided by the present invention is to evaluate the degree of hangover after drinking in the time period from the abnormal excitement state to the fully awake state after simulating excessive but not drunken drinking of human body, which breaks through the difficulty of completely inhibiting the behavior of animals caused by the conventional evaluation method under the dose of complete drunkenness and failing to observe the behavior changes under the condition of not being completely drunk, and broadens the evaluation system of the comfort degree after drinking alcohol. The present invention can compare and analyze the degree of hangover after drinking different alcohols by performing data analysis with the hangover value of the control group (the alcohol drinking group or the comparison of different alcohol products). DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the embodiments of the present invention belong. If the definitions set forth in this section are contrary to or otherwise inconsistent with the definitions set forth in the patents, patent applications, published patent applications, and other publications incorporated herein by reference, the definitions listed in this section take precedence over the definitions incorporated herein by reference.

[0024] Unless otherwise specified, the methods used in the following examples are all conventional methods. Unless otherwise specified, the materials, reagents and instruments used are all conventional materials, reagents and instruments in the art, and can be obtained by those skilled in the art through commercial channels.

[0025] When an amount, concentration or other value or parameter is expressed as a range, a preferred range or a range defined by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including the range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values ​​and all integers and fractions within the range. In the present specification and claims, range definitions can be combined and / or interchanged, and if not otherwise stated, these ranges include all subranges contained therein.

[0026] Example 1

[0027] This example is an evaluation of the severity of hangover after drinking alcohol solutions with different hangover extracts under the same alcohol intake. The grouping, test alcohol sample information, and changes in the autonomous activity distance of the tested alcohol sample mice at different time points after drinking and the hangover degree values ​​are as follows:

[0028] (1) Male C57BL / 6j mice with good adaptability to feeding were selected and randomly divided into 7 groups according to body weight, with 10 mice in each group. 6 kinds of 25-degree alcohol products and edible alcohol were added with extracts of different hangover degrees after drinking, a total of 7 kinds of alcohol, namely 25-degree alcohol, Chuanxiong, processed Polygonatum, Allium seeds, Codonopsis, Lycium barbarum, and Rhodiola rosea. The intragastric volume of 25-degree alcohol was determined to be 0.15ml / 10g·bw.

[0029] (2) According to the experimental groups, specifically, when the blood ethanol content of mice reached its peak about 0.5 h after oral gavage, the mice were relatively hyperactive at this time, and the activity level remained stable until the blood ethanol content of mice reached 70-90% of the peak value; until the blood ethanol content of mice reached 60-70% of the peak value, the activity level decreased and began to stabilize, and thereafter when the blood ethanol content of mice reached 40-60% of the peak value, the activity level remained stable; until the blood ethanol content reached 20-30% of the peak value, the mice were basically awake and the activity level was stable; when the blood ethanol content reached 0-10% of the peak value, the mice were completely awake and the activity level was stable with no difference. Therefore, the T1 time point is set as the peak moment of the mouse blood ethanol content, the M1 time point is set as the moment when the mouse blood ethanol content reaches 70-90% of the peak, the T2 time point is set as the moment when the mouse blood ethanol content reaches 60-70% of the peak, the M2 time point is set as the moment when the mouse blood ethanol content reaches 40-60% of the peak, the T3 time point is set as the moment when the mouse blood ethanol content reaches 20-30% of the peak, and the M3 time point is set as the moment when the mouse blood ethanol content reaches 0-10% of the peak. The activity distance of the mouse within the single activity duration range after the corresponding time point is calculated respectively, and the obtained experimental data is analyzed to obtain the hangover degree value after drinking = activity distance 1 (time point T1) + activity distance 2 (time point T2) + activity distance 3 (time point T3). The unit of the activity distance is decimeter (dm), and the single activity duration is 20 minutes. Taking the hangover degree value of the alcohol drinking group as 100% as the benchmark, the increase and decrease range of the hangover degree values ​​of alcoholic products are graded as follows: wines with a decrease of ≥10% (≤90%) are regarded as wines with a reduced hangover degree after drinking; wines with an increase of ≥10% (≥110%) are regarded as wines with aggravated hangover degree after drinking; wines with an increase and decrease within 10% (90%-110%) are regarded as wines with no significant difference in hangover degree after drinking.

[0030] After statistics, the data results are shown in the following table:

[0031]

[0032]

[0033] serial number T1 ethanol mmol / L T2 ethanol mmol / L T3 ethanol mmol / L Ethanol metabolism capacity 0 111.30±8.90 79.90±3.10 31.04±9.81 middle 1 99.01±8.44* 67.64±5.55 28.01±5.93 powerful 2 103.65±7.70 73.59±8.31 27.29±7.84 powerful 3 107.99±11.86 69.43±9.79 29.31±10.21 middle 4 112.44±7.97 78.24±7.80 30.74±9.53 middle 5 121.53±10.15* 80.97±8.06* 31.04±9.81 weak 6 125.33±8.74* 75.4±4.95 33.17±6.07 weak

[0034] serial number M1 ethanol mmol / L M2 ethanol mmol / L M3 ethanol mmol / L Ethanol metabolism capacity 0 98.62±10.85 58.15±8.35 9.17±3.06 middle 1 85.55±6.54* 42.27±8.78* 9.24±2.33 powerful 2 91.93±6.90 41.75±12.37 10.73±3.55 powerful 3 97.96±6.46 55.5±9.58 10.17±4.62** middle 4 103.97±5.06 53.5±8.30 11.08±3.63** middle 5 104.93±7.68 61.33±3.82 10.50±3.91 weak 6 109.38±5.63 62.85±8.43 11.70±4.47 weak

[0035] The data in the above table show that the changes in the autonomous activity distance of mice after drinking at T1, T2, and T3 time points can accurately assess the severity of hangover after drinking. Compared with the control group (25-degree alcohol), the degree of hangover after drinking Rhodiola rosea was significantly reduced, as shown by a significant decrease in the total distance of autonomous activity at different time points after drinking, indicating that the hangover discomfort symptoms of abnormal excitement and anxiety in animals facing a new environment after drinking a moderate amount of this wine were significantly alleviated. The degree of hangover after drinking Chuanxiong and Cinnamon was significantly aggravated, as shown by a significant increase in the total distance of autonomous activity at different time points after drinking, indicating that the hangover discomfort symptoms of abnormal excitement and anxiety in animals facing a new environment after drinking a moderate amount of this wine were significantly aggravated.

[0036] Example 2

[0037] This example is an evaluation of the severity of hangover after drinking alcohol solutions with different hangover extracts under the same alcohol intake. The grouping, test alcohol sample information, and changes in the autonomous activity distance of the tested alcohol sample mice at different time points after drinking and the hangover degree values ​​are as follows:

[0038] (1) Male C57BL / 6j mice with good adaptability to feeding were selected and randomly divided into 6 groups according to body weight, with 10 mice in each group. Five kinds of 42-degree liquor products containing extracts of different hangover degrees after drinking and edible alcohol were added, a total of 6 kinds of liquor, namely 42-degree alcohol and another 5 kinds of 42-degree liquor products. The gavage volume of 42-degree liquor was determined to be 0.06ml / 10g·bw.

[0039] (2) According to the experimental groups, the autonomous activities of mice in each group were monitored and the blood ethanol content was detected within 0.5-8 hours after a single intragastric administration. Since there is a certain regularity between the activity performance of mice and the performance of blood ethanol content, specifically, when the blood ethanol content of mice reaches a peak value about 0.5 hours after intragastric administration, the mice are relatively active at this time. Thereafter, until the blood ethanol content of mice reaches 60-70% of the peak value, the activity decreases and begins to stabilize. When the blood ethanol content reaches 20-30% of the peak value, the mice are basically awake and the activity is stable. Therefore, the T1 time point is set as the peak moment of the blood ethanol content of mice, the T2 time point is set as the moment when the blood ethanol content of mice reaches 60-70% of the peak value, and the T3 time point is set as the moment when the blood ethanol content of mice reaches 20-30% of the peak value. The activity distance of mice within the single activity duration after the corresponding time point is calculated respectively, and the obtained experimental data is analyzed to obtain the hangover degree value after drinking = activity distance 1 (time point T1) + activity distance 2 (time point T2) + activity distance 3 (time point T3). The unit of activity distance is decimeter (dm), and the duration of a single activity is 20 minutes. Taking the hangover degree value of the alcohol drinking group as 100% as the benchmark, the increase and decrease of the hangover degree value of alcoholic products are classified as follows: the wine with a decrease of ≥10% (≤90%) is regarded as the wine with a reduced hangover degree after drinking; the wine with an increase of ≥10% (≥110%) is regarded as the wine with aggravated hangover degree after drinking; the wine with an increase and decrease within 10% (90%-110%) is regarded as the wine with no significant difference in hangover degree after drinking.

[0040] After statistics, the data results are shown in the following table:

[0041]

[0042]

[0043]

[0044] It can be seen from the above table that compared with the control group (42% alcohol), the degree of hangover after drinking 42% liquor 1 and 2 was significantly reduced, as shown by the significant decrease in the total distance of autonomous activity at different time points after drinking, indicating that the hangover discomfort symptoms of abnormal excitement and anxiety of animals facing a new environment after drinking a moderate amount of this liquor were significantly alleviated. The degree of hangover after drinking 42% liquor 5 was significantly aggravated, as shown by the significant increase in the total distance of autonomous activity at different time points after drinking, indicating that the hangover discomfort symptoms of abnormal excitement and anxiety of animals facing a new environment after drinking a moderate amount of this liquor were significantly aggravated.

[0045] In summary, the evaluation method and application of the degree of hangover after drinking alcoholic products in the embodiment of the present application is to simulate the degree of hangover after drinking in the time period from the abnormal excitement state to the fully awake state after drinking at an excessive but not drunken dose of human body, breaking through the difficulty of completely inhibiting the animal behavior caused by the conventional evaluation method under the dose of complete drunkenness and failing to observe the behavioral changes in the case of not being completely drunk, and establishing a method for detecting the degree of hangover after drinking by comparing and observing the increase and decrease of autonomous activities at different time points after drinking alcoholic drinks with different hangover degrees, and broadening the evaluation system of comfort after drinking alcoholic drinks. The present invention is to perform data analysis by comparing the hangover values ​​of the control group (alcohol drinking group or different alcoholic products), and can compare and analyze the degree of hangover after drinking different alcoholic drinks. The method has the advantages of simple operation, high sensitivity, and strong practicality.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for evaluating the degree of hangover after drinking, characterized in that: The steps include: Step 1: Divide the test mice into an experimental group and a control group. The mice in the control group are gavaged with alcohol once, and the mice in the experimental group are gavaged with different alcoholic products once. The total amount of alcohol in the alcoholic products gavaged once by the mice in the control group and the mice in the experimental group is the same; Step 2: At different time points, observe the changes in the autonomic activity of the mice in the control group and the experimental group, and score the autonomic activity. According to the score, evaluate the degree of hangover of the corresponding alcohol consumed by the mice.

2. The method for evaluating the degree of hangover after drinking according to claim 1, characterized in that: The single gavage volume is 0.06-0.15 ml / 10 g·bw.

3. The method for evaluating the degree of hangover after drinking according to claim 1, characterized in that: The different time points in step 2 are different time points within 0.5-8 hours after a single intragastric administration.

4. The method for evaluating the degree of hangover after drinking according to claim 1, characterized in that: In step 2, the autonomous activity scoring method includes: counting the corresponding activity distances of the mice within a single activity duration starting from the corresponding time points at three different time points T1, T2 and T3, respectively, recording them as D1, D2 and D3, calculating the sum of D1, D2 and D3, taking the sum of D1, D2 and D3 of the control group as 100%, calculating the percentages of different test groups relative to the control group, and evaluating the degree of hangover of the alcohol according to the percentage values ​​of the test groups relative to the control group.

5. The method for evaluating the degree of hangover after drinking according to claim 4, characterized in that: The method for evaluating the specific degree of hangover includes: when the percentage value of the test group relative to the control group is ≤90%, the wine is regarded as the wine with reduced hangover after drinking; when the percentage value of the test group relative to the control group is ≥110%, the wine is regarded as the wine with aggravated hangover after drinking; when the percentage value of the test group relative to the control group is >90% and <110%, the wine is regarded as the wine with no significant difference in hangover after drinking.

6. The method for evaluating the degree of hangover after drinking according to claim 4, characterized in that: Step 2 also includes detecting the blood ethanol content of the mice at different time points.

7. The method for evaluating the degree of hangover after drinking according to claim 6, characterized in that: The T1 time point corresponds to the time point when the mouse blood ethanol content reaches the highest point, the T2 time point corresponds to the time point when the mouse blood ethanol content reaches 60-70% of the highest point, and the T3 time point corresponds to the time point when the mouse blood ethanol content reaches 20-30% of the highest point.

8. Use of the method for evaluating the degree of hangover after drinking according to any one of claims 1 to 7, characterized in that: For screening of products that improve drinking comfort.

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