Nutritional composition beneficial to bone health and height development and application thereof

Abstract

The invention discloses a nutritional composition. The nutritional composition comprises vitamin D, basic protein and lysine, wherein in the nutritional composition, the mass ratio of the vitamin D to the basic protein to the lysine is (0.005 to 0.020) to (70 to 200) to (1000 to 3000). The invention also discloses an application of the nutritional composition and a formula food containing the nutritional composition. According to the nutritional composition disclosed by the invention, the vitamin D, the basic protein and the lysine can be matched with one another, so that linear growth of bones of children and teenagers can be effectively promoted, bone health and bone strength can be enhanced, and height development can be promoted.

Description

Technical Field

[0001] The invention relates to the technical field of nutritional products, and in particular to a nutritional composition that is helpful for bone health and height development and application thereof. Background Art

[0002] Osteoporosis (OP) is a systemic metabolic bone disease characterized by decreased bone mass, increased bone brittleness, and deterioration of bone microstructure, which leads to an increased risk of fractures. An effective and economical way to reduce the incidence of osteoporosis is to increase bone mass during childhood and adolescence to achieve optimal peak bone mass (PBM). Studies have shown that for every 10% increase in PBM, the risk of osteoporotic fractures in the future will be reduced by 50%, and the age of onset of osteoporosis will be delayed by 13 years.

[0003] The peak bone mass reached during bone maturation and the rate of bone loss in adulthood are two major factors affecting the risk of osteoporosis. Previous studies on osteoporosis focused on exploring interventions to reduce bone loss in adulthood, but in recent years the focus has shifted to how to maximize the peak bone mass during growth and development, thereby preventing and delaying the onset of osteoporosis in adulthood.

[0004] During childhood and adolescence, bones grow and develop rapidly, mainly bone construction, bone turnover is accelerated, and bone formation is dominant, so bone mass grows steadily. Bone growth includes linear growth and bone mass accumulation. Among them, linear growth refers to the growth of an organism at a constant rate within a certain period of time. This growth pattern can be described by a linear function y=kx+b, where y is the growth amount, x is the time, k is the growth rate, and b is the initial value. Linear growth can be reflected in multiple physiological indicators, such as weight, height, and the weight of certain organs. Linear growth is the main growth in childhood, with an average growth rate of about 5~6cm per year. During adolescence, especially puberty, bone mass accumulation is the main focus. About 40%~60% of adult bone mass is achieved during adolescence, and 90% of peak bone mass has been accumulated by the age of 18. Bone mineral content and bone density gradually increase with bone growth, and peak bone mass will be reached in adulthood. The bone mass in childhood and adolescence determines the peak bone mass in a person's lifetime.

[0005] Bone is a metabolically active organ that is constantly remodeled and metabolized throughout a person's life. Bone remodeling refers to a cyclic metabolic process in which a small amount of bone occurs in the same part of the bone. Bone formation and bone resorption are carried out to maintain the relative stability of the bone. Bone accumulation is completed during the process of bone formation and bone resorption. Bone is composed of approximately 10% water, 30% organic matter and 60% inorganic matter. Approximately 85% to 90% of the organic component is collagen. The inorganic component or mineralized matrix is ​​mainly composed of hydroxyapatite, which provides protection and support, and serves as a reservoir for calcium and phosphate in the human body. Calcium is closely related to the strength and structure of bones, and protein is an important raw material for the synthesis of bone organic matter. Factors affecting bone mass include genetic and environmental factors, and nutrition is an important environmental factor affecting peak bone mass.

[0006] The cartilage growth plate (CGP) is a cartilage tissue located between the epiphysis and the diaphysis. It is the main differentiation area for the longitudinal growth of bones and is mainly composed of chondrocytes and extracellular matrix. In childhood, the proliferation and degeneration of cartilage and the rate of new bone formation are balanced. This ensures that the growth plate can maintain a certain thickness while the length of the diaphysis increases. When the cartilage growth plate develops to the mature stage, the cartilage proliferation and osteogenic activity stop, the growth plate is completely ossified, and the longitudinal growth of the bone stops. Therefore, the cartilage growth plate regulates and controls the longitudinal growth rate of the bone and the final length of the bone. There are many factors related to CGP and regulating growth, mainly including: growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis, multiple hormones, paracrine factors, inflammatory cytokines, etc., which can cause chondrocytes to proliferate and differentiate to form secondary ossification centers, causing the longitudinal growth of long bones.

[0007] Therefore, when exploring compositions suitable for bone health and height development in children and adolescents, it is necessary to consider both bone mass accumulation and linear growth. Studies have demonstrated the positive role of nutrients such as calcium, vitamin D, and protein in promoting bone mass accumulation, but the role of nutrients in linear growth in children and adolescents is unclear, nor is the synergistic effect between several key compositions. For children and adolescents, linear growth is crucial for height development. Supplementing with innovative nutritional compositions to help children and adolescents improve bone health and increase linear growth is of great significance in promoting growth and development. Summary of the invention

[0008] The technical problem to be solved by the present invention is to provide a nutritional composition, in which the three components of vitamin D, basic protein and lysine can cooperate with each other to effectively promote the linear growth of bones in children and adolescents, increase bone mass, enhance bone density and bone strength, and promote height development.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0010] A first aspect of the present invention provides a nutritional composition comprising vitamin D, basic protein and lysine; wherein, in the nutritional composition, the mass ratio of vitamin D, basic protein and lysine is (0.005~0.020): (70~200): (1000~3000).

[0011] Vitamin D is a fat-soluble vitamin, the most important of which are vitamin D3 (cholecalciferol, cholecalciferol) and vitamin D2 (calciferol). Vitamin D can regulate calcium and phosphorus metabolism, promote intestinal calcium and phosphorus absorption and bone calcification, and maintain the balance of blood calcium and blood phosphorus.

[0012] Basic protein is a type of small molecule active protein extracted from the milk of mammals such as cow's milk, human milk, goat's milk, and Mianyang milk. It is a trace component in whey and has a special effect on strengthening bones. Basic protein can directly act on bone cells to promote bone metabolism; it can coordinate the activities of osteoblasts and osteoclasts, maintain a dynamic balance between the two, promote bone growth, and repair bones.

[0013] Lysine is one of the essential amino acids for the human body. It can participate in the synthesis of various proteins in the body, such as skeletal muscle, enzymes, serum proteins, polypeptide hormones, etc. It can also chelate with mineral elements such as calcium and iron to form soluble small molecule monomers, promote the absorption of these mineral elements, and accelerate bone growth.

[0014] In childhood and adolescence, the linear growth of bones plays a vital role in height development. Although the role of individual vitamin D, basic protein and lysine in promoting calcium absorption, bone development and bone mass accumulation has been extensively studied in the prior art, the role of vitamin D, basic protein and lysine in the linear growth of bones in children and adolescents, and whether these components have a synergistic effect in promoting bone health and height development, the prior art is still unclear.

[0015] The inventors found in their research that when vitamin D, basic protein and lysine are used in a certain ratio, the components can cooperate with each other and achieve a synergistic effect in promoting the height development of children and adolescents, and can better promote the linear growth of bones in children and adolescents, increase bone mass, and enhance bone density and bone strength. In addition, vitamin D, basic protein and lysine need to be compounded in a ratio of (0.005~0.020): (70~200): (1000~3000) to achieve the above synergistic effect. When the ratio of vitamin D, basic protein and lysine is outside the above range, the corresponding effect will be significantly reduced.

[0016] Furthermore, the alkaline protein includes but is not limited to at least one of colostrum alkaline protein and milk alkaline protein.

[0017] Colostrum basic protein (CBP) is a small molecule active peptide made from colostrum. It is sterilized, defatted, centrifuged, casein, α-lactalbumin and β-lactoglobulin are removed, and then processed by microfiltration, ultrafiltration, freeze-drying and other processes.

[0018] Milk basic protein (MBP) is made from fresh milk through processes such as defatting, filtration, concentration, removal of acidic proteins such as casein, cationic chromatography, and freeze-drying.

[0019] More preferably, the basic protein is derived from milk-derived basic protein of bovine colostrum, namely, colostrum basic protein.

[0020] In the present invention, the mass ratio of vitamin D, basic protein and lysine is (0.005-0.020):(70-200):(1000-3000), for example, it can be 0.005:70:1000, 0.01:100:1500, 0.015:150:2000, 0.02:200:3000 and the like.

[0021] Further preferably, in the nutritional composition, the mass ratio of vitamin D, basic protein and lysine is (0.005~0.020):(100~200):(1500~3000).

[0022] In a second aspect, the present invention also provides the use of the nutritional composition in preparing a preparation for improving bone health and / or promoting height development.

[0023] In a third aspect, the present invention also provides the use of the nutritional composition in the preparation of a preparation for promoting linear bone growth in children and adolescents.

[0024] In a fourth aspect, the present invention also provides the use of the nutritional composition in preparing a preparation for increasing the relative volume, thickness and number of trabeculae, and reducing the trabecular pattern factor and trabecular separation.

[0025] In a fifth aspect, the present invention also provides the use of the application composition in the preparation of a preparation for increasing the levels of growth hormone, insulin-like growth factor, estrogen and estrogen receptor.

[0026] In a sixth aspect, the present invention provides a formula food, wherein the formula food comprises the above-mentioned nutritional composition.

[0027] Furthermore, in the formula food, the content of vitamin D is 0.005-0.020 mg / 100 g, the content of basic protein is 70-200 mg / 100 g, and the content of lysine is 1000-3000 mg / 100 g. Further preferably, in the formula food, the content of vitamin D is 0.005-0.020 mg / 100 g, the content of basic protein is 100-200 mg / 100 g, and the content of lysine is 1500-3000 mg / 100 g.

[0028] Furthermore, in the formula food, the source of vitamin D includes but is not limited to at least one of ergocalciferol and cholecalciferol; the source of lysine includes but is not limited to at least one of milk powder, whey protein powder and L-lysine. Preferably, the whey protein powder is isolated whey protein powder.

[0029] Furthermore, the formula food includes but is not limited to infant food, children's food, adolescent food or adult food.

[0030] Furthermore, the formula food is infant formula milk powder, baby complementary food, nutrition or dietary supplement, children's formula milk powder, children's snacks or health food for the middle-aged and elderly.

[0031] Furthermore, in the formula food, the total protein content is 10-30 g / 100 g, the fat content is 8-20 g / 100 g, and the dietary fiber content is 1.0-8 g / 100 g. Preferably, in the formula food, the total protein content is 22.8 g / 100 g, the fat content is 12 g / 100 g, and the dietary fiber content is 3.0 g / 100 g.

[0032] Furthermore, the formula food may also include one or more of the compound nutrients consisting of prebiotics, lactose, DHA, vitamins and minerals.

[0033] Furthermore, in some embodiments, the formula food can be prepared from the following components by weight:

[0034] 0~2000 parts of raw milk, 0~200 parts of whole milk powder, 0~325 parts of skimmed milk powder, 10~100 parts of lactose, 0~375 parts of desalted whey powder, 0~10 parts of oligofructose, 0~10 parts of inulin, 0~5 parts of calcium citrate, 0~2 parts of complex mineral premix, 0.1~2 parts of complex vitamin premix, 0~10 parts of DHA, 0~6.5 parts of lactoferrin, 0.01~2 parts of basic protein, 0~5 parts of milk mineral salt, 0~5 parts of HMO, 0~2 parts of lutein ester, 0~5 parts of hydrolyzed egg yolk powder, 0~5 parts of choline bitartrate, 0~5 parts of fruit and vegetable mixed powder, 0~10 parts of isolated whey protein powder, 0~5 parts of N-acetylneuraminic acid, 0~0.2 parts of probiotics, and 0~1 parts of nucleotides.

[0035] In the above formula, the usage of raw milk, whole milk powder and skimmed milk powder is not all zero.

[0036] Furthermore, in some embodiments, the raw materials of the fruit and vegetable mixed powder include apples, hawthorns and red dates.

[0037] Furthermore, in some embodiments, the composite mineral premix comprises calcium citrate, ferrous sulfate, zinc sulfate, magnesium oxide, manganese sulfate, copper sulfate and sodium selenite.

[0038] Furthermore, in some embodiments, the multivitamin premix comprises vitamin D3, sodium L-ascorbate, vitamin K2, dl-α-tocopherol acetate, calcium D-pantothenate, niacinamide, folic acid, cyanocobalamin, thiamine hydrochloride, pyridoxine hydrochloride, vitamin A acetate and choline bitartrate.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] 1. The nutritional composition provided by the present invention is prepared by compounding vitamin D, basic protein and lysine in a ratio of (0.005-0.020): (70-200): (1000-3000). The three components of vitamin D, basic protein and lysine in the nutritional composition can cooperate with each other to achieve a synergistic effect in promoting the height development of children and adolescents, and can better promote the linear growth of bones in children and adolescents, increase bone mass, and enhance bone density and bone strength.

[0041] 2. The nutritional composition provided by the present invention can be applied to formula foods, can improve bone strength, and contribute to bone health and height development. DETAILED DESCRIPTION

[0042] The present invention is further described below in conjunction with specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0043] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0044] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are all conventional methods, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0045] In the following embodiments, the content of vitamin D in the compound vitamin premix can be customized according to the content of vitamin D in the corresponding formula milk powder. The fruit and vegetable mixed powder was purchased from Kangmaide Company, and the product model is fruiVeg series Red blend.

[0046] Example 1

[0047] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportions of the raw materials are shown in Table 1.

[0048] Table 1

[0049] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 40.950 2 Skim milk powder kg 34.000 3 lactose kg 12.769 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.070 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 2.500 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0050] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.005mg / 100g, CBP content is 70mg / 100g; based on effective substances, the lysine content is calculated to be 1000mg / 100g.

[0051] The formula milk powder of the present embodiment, its preparation method is as follows:

[0052] (1) Mixing: Mix all the above raw materials to obtain a uniform liquid; the mixing temperature is 50°C and the mixing time is 50 min;

[0053] (2) Homogenization: homogenize the liquid at a homogenization pressure of 115 MPa and a homogenization temperature of 55 °C;

[0054] (3) Pasteurization: The homogenized liquid is sterilized at a temperature of 91°C and a time of 15 seconds;

[0055] (4) Concentration: Concentrate the sterilized liquid at a temperature of 50°C until the liquid concentration reaches 49%;

[0056] (5) Spray drying: The concentrated liquid was spray dried with an inlet air temperature of 191°C and an exhaust air temperature of 89°C to obtain a dry and uniform powder, which was recorded as nutritional fortification formula A.

[0057] Example 2

[0058] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 2.

[0059] Table 2

[0060] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 51.615 2 Skim milk powder kg 16.346 3 lactose kg 12.689 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein kg 0.150 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 9.489 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 1.000 total kg 100.000

[0061] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.005mg / 100g, CBP content is 150mg / 100g; based on effective substances, the lysine content is calculated to be 3000mg / 100g.

[0062] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula B.

[0063] Example 3

[0064] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 3.

[0065] Table 3

[0066] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.139 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.200 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 0.5 total kg 100.000

[0067] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.005mg / 100g, CBP content is 200mg / 100g; based on effective substances, the lysine content is calculated to be 2000mg / 100g.

[0068] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula C.

[0069] Example 4

[0070] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 4.

[0071] Table 4

[0072] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 51.615 2 Skim milk powder kg 16.346 3 lactose kg 12.769 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein kg 0.070 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 9.489 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 1. 000 total kg 100.000

[0073] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.010 mg / 100 g, CBP content is 70 mg / 100 g; based on effective substances, the lysine content is calculated to be 3000 mg / 100 g.

[0074] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula D.

[0075] Example 5

[0076] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 5.

[0077] Table 5

[0078] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.739 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.100 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0079] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.010 mg / 100 g, and CBP content is 100 mg / 100 g; based on effective substances, the lysine content is calculated to be 1500 mg / 100 g.

[0080] The formula milk powder of this example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula E.

[0081] Example 6

[0082] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 6.

[0083] Table 6

[0084] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 40.950 2 Skim milk powder kg 34.000 3 lactose kg 12.639 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.200 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 2.500 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0085] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.010 mg / 100 g, CBP content is 200 mg / 100 g; based on effective substances, the lysine content is calculated to be 1000 mg / 100 g.

[0086] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula F.

[0087] Example 7

[0088] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 7.

[0089] Table 7

[0090] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.739 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.100 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0091] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.015mg / 100g, CBP content is 100mg / 100g; based on effective substances, the lysine content is calculated to be 1500mg / 100g.

[0092] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula G.

[0093] Example 8

[0094] This embodiment provides a formula milk powder that helps bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of each raw material are shown in Table 8.

[0095] Table 8

[0096] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.689 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.150 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 0.5 total kg 100.000

[0097] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.015mg / 100g, CBP content is 150mg / 100g; based on effective substances, the lysine content is calculated to be 2000mg / 100g.

[0098] The formula milk powder of this example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula H.

[0099] Example 9

[0100] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, milk alkaline protein, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 9.

[0101] Table 9

[0102] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.189 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Milk basic protein kg 0.150 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 0.5 total kg 100.000

[0103] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.015mg / 100g, and milk alkaline protein content is 150mg / 100g; based on effective substances, the lysine content is calculated to be 2000mg / 100g.

[0104] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula I.

[0105] Example 10

[0106] This embodiment provides a formula milk powder that helps bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 10.

[0107] Table 10

[0108] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 51.615 2 Skim milk powder kg 16.346 3 lactose kg 12.689 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein kg 0.150 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 9.489 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 1. 000 total kg 100.000

[0109] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.015mg / 100g, CBP content is 150mg / 100g; based on effective substances, the lysine content is calculated to be 3000mg / 100g.

[0110] The formula milk powder of this example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula J.

[0111] Embodiment 11

[0112] This embodiment provides a formula milk powder that helps bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 11.

[0113] Table 11

[0114] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.269 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.070 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 0.5 total kg 100.000

[0115] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.020mg / 100g, CBP content is 70mg / 100g; based on effective substances, the lysine content is calculated to be 2000mg / 100g.

[0116] The formula milk powder of this example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula K.

[0117] Example 12

[0118] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 12.

[0119] Table 12

[0120] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 40.950 2 Skim milk powder kg 34.000 3 lactose kg 12.739 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.100 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 2.500 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0121] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.020mg / 100g, CBP content is 100mg / 100g; based on effective substances, the lysine content is calculated to be 1000mg / 100g.

[0122] The formula milk powder of this embodiment was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula L.

[0123] Embodiment 13

[0124] The present embodiment provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportion of each raw material is shown in Table 13.

[0125] Table 13

[0126] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 51.615 2 Skim milk powder kg 16.346 3 lactose kg 12.639 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein kg 0.200 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 9.489 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 1. 000 total kg 100.000

[0127] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.020mg / 100g, CBP content is 200mg / 100g; based on effective substances, the lysine content is calculated to be 3000mg / 100g.

[0128] The formula milk powder of this embodiment is prepared in the same manner as in Example 1 and is recorded as nutritionally fortified formula M.

[0129] Comparative Example 1

[0130] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 14.

[0131] Table 14

[0132] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.839 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0133] The main nutritional components of the formula milk powder in this comparative example are as follows: protein content 38%, fat content 20.0%, dietary fiber content 12%, and lysine content of 1500 mg / 100 g.

[0134] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula N.

[0135] Comparative Example 2

[0136] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum alkaline protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 15.

[0137] Table 15

[0138] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.901 2 Skim milk powder kg 37.061 3 lactose kg 12.741 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.100 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0139] The main nutritional components of the formula milk powder of this comparative example are as follows: protein content 38%, fat content 20.0%, dietary fiber content 12%, CBP content 100 mg / 100 g, and lysine content 1500 mg / 100 g.

[0140] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula O.

[0141] Comparative Example 3

[0142] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 16.

[0143] Table 16

[0144] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.839 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0145] The main nutritional components of the formula milk powder in this comparative example are as follows: protein content 38%, fat content 20.0%, dietary fiber content 12%; calculated on the basis of effective substances, the content of vitamin D3 is 0.010 mg / 100 g, and the content of lysine is calculated to be 1500 mg / 100 g.

[0146] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as the nutritionally fortified formula P.

[0147] Comparative Example 4

[0148] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 17.

[0149] Table 17

[0150] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.739 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.100 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0151] The main nutritional components of the formula milk powder of this comparative example are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.002mg / 100g, CBP content is 100mg / 100g, and lysine content is 1500mg / 100g.

[0152] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula Q.

[0153] Comparative Example 5

[0154] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum alkaline protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 18.

[0155] Table 18

[0156] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.799 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.040 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0157] The main nutritional components of the formula milk powder of this comparative example are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.010 mg / 100g, and CBP content is 40 mg / 100g; calculated on the basis of effective substances, the lysine content is calculated to be 1500 mg / 100g.

[0158] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula R.

[0159] Comparative Example 6

[0160] This comparative example provides a formula milk powder that is helpful for bone health and height development, which is prepared from the following raw materials: whole milk powder, lactose, inulin, oligofructose, maltodextrin, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, fruit and vegetable mixed powder and N-acetylneuraminic acid. The proportion of each raw material is shown in Table 19.

[0161] Table 19

[0162] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 53.350 2 Skim milk powder kg 0 3 lactose kg 12.719 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Maltodextrin kg 24.12 7 Calcium Citrate kg 2.300 8 2081289 Children's formula milk powder mineral premix kg 0.516 9 2081288 Children's formula milk powder vitamin premix kg 0.240 10 Colostrum Basic Protein CBP kg 0.100 11 Milk mineral salt kg 0.800 12 2'-fucosyllactose kg 0.500 13 Lutein ester microencapsulated powder kg 0.010 14 Hydrolyzed Egg Yolk Powder kg 0.030 15 DHA Algae Oil Powder kg 0.470 16 Lactoferrin kg 0.030 17 Choline bitartrate kg 0.240 18 Whey Protein Isolate Powder kg 0 19 Fruit and vegetable mixed powder kg 0.285 20 N-Acetylneuraminic acid kg 0.040 total kg 100.000

[0163] The main nutritional components of the formula milk powder of this comparative example are as follows: protein content is 34%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.010 mg / 100g, and CBP content is 100 mg / 100g; calculated on the basis of effective substances, the lysine content is calculated to be 800 mg / 100g.

[0164] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula S.

[0165] Comparative Example 7

[0166] This embodiment provides a formula milk powder that helps bone health and height development, which is prepared from the following raw materials: whole milk powder, skim milk powder, lactose, inulin, oligofructose, calcium citrate, compound mineral premix, compound vitamin premix, colostrum basic protein CBP, milk mineral salt, 2'-fucosyllactose, lutein ester microcapsule powder, hydrolyzed egg yolk powder, DHA algae oil powder, lactoferrin, choline bitartrate, isolated whey protein powder, fruit and vegetable mixed powder, N-acetylneuraminic acid and L-lysine. The proportions of the raw materials are shown in Table 20.

[0167] Table 20

[0168] Serial number Raw material description unit Addition amount (%) 1 Whole milk powder kg 38.903 2 Skim milk powder kg 37.061 3 lactose kg 12.279 4 Inulin kg 2.150 5 Fructo-oligosaccharides kg 2.100 6 Calcium Citrate kg 2.300 7 2081289 Children's formula milk powder mineral premix kg 0.516 8 2081288 Children's formula milk powder vitamin premix kg 0.240 9 Colostrum Basic Protein CBP kg 0.060 10 Milk mineral salt kg 0.800 11 2'-fucosyllactose kg 0.500 12 Lutein ester microencapsulated powder kg 0.010 13 Hydrolyzed Egg Yolk Powder kg 0.030 14 DHA Algae Oil Powder kg 0.470 15 Lactoferrin kg 0.030 16 Choline bitartrate kg 0.240 17 Whey Protein Isolate Powder kg 1.486 18 Fruit and vegetable mixed powder kg 0.285 19 N-Acetylneuraminic acid kg 0.040 20 L-Lysine kg 0.5 total kg 100.000

[0169] The main nutritional components of the formula milk powder of this embodiment are as follows: protein content is 38%, fat content is 20.0%, dietary fiber content is 12%, vitamin D3 content is 0.008mg / 100g, CBP content is 60mg / 100g; based on effective substances, the lysine content is calculated to be 2000mg / 100g.

[0170] The formula milk powder of this comparative example was prepared in the same manner as in Example 1 and was recorded as nutritionally fortified formula T.

[0171] Test example

[0172] 1. Research objectives

[0173] To investigate the effects of specific nutritional compositions on improving bone strength, enhancing bone health and linear growth and development.

[0174] 2. Research Materials and Research Design

[0175] 2.1 Main instruments and equipment

[0176] Table 21

[0177] Name of instrument Manufacturer Vernier caliper Green Forest Tools Co., Ltd. Electronic balance Ohaus Instruments (Shanghai) Co., Ltd. Micropipette Eppendorf (China) Co., Ltd. Low temperature high speed centrifuge Eppendorf (China) Co., Ltd. 4℃, refrigerator Hefei Meiling Co., Ltd. -80℃ Ultra Low Temperature Refrigerator (USA) Thermo Fisher Scientific Microplate reader, Infinite 200 Pro reader Tecan, Männedorf, Switzerland Small animal micro-CT, SkyScan 1176 (Germany) Bruker Corporation (NASDAQ:BRKR)

[0178] 2.2 Main reagents and materials

[0179] Table 22

[0180] Reagent material name Reagent Material Company AIN-93M Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd. AIN-93G Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd. Corn cob litter Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd. 4% paraformaldehyde Wuhan Bolfu Biotechnology Co., Ltd. Glucose Determination Kit (Glucose Oxidase Method) Sinopharm Biotech Co., Ltd. Mouse osteocalcin (BGP; OCN) ELISA kit Wuhan Zhongkeyou Biotechnology Co., Ltd. Mouse Growth Hormone (GH) ELISA Kit Wuhan Zhongkeyou Biotechnology Co., Ltd. Mouse Insulin-like Growth Factor 1 (IGF-1) ELISA Kit Wuhan Zhongkeyou Biotechnology Co., Ltd. Mouse Estrogen (E2) ELISA Kit Wuhan Zhongkeyou Biotechnology Co., Ltd. Mouse Estrogen Receptor α (ERα) ELISA Kit Wuhan Zhongkeyou Biotechnology Co., Ltd.

[0181] Study design

[0182] 440 3-week-old (early puberty) C57BL / 6J mice (220 females and 220 males) were used. After one week of adaptive feeding, they were randomly divided into 22 groups, namely, blank control group, positive control group, intervention groups 1-13, and comparative intervention groups 14-20. Each group had 20 mice, with males and females evenly distributed, and given different feeding treatments (see Table 23). During the intervention period, the mice ate freely, and it was determined experimentally that there was no difference in the total food intake of the mice in each group during the experiment.

[0183] Table 23

[0184] Animal Experiment Example Dosage Group Blank control group AIN-93G_D10012M Basic feed Positive control group AIN-93G_D10012G Growth and development feed Intervention Group 1 Basic feed + nutritional fortification formula A Intervention Group 2 Basic feed + nutritional fortification formula B Intervention Group 3 Basic feed + nutritional fortification formula C Intervention Group 4 Basic feed + nutritional fortification formula D Intervention Group 5 Basic feed + nutritional fortification formula E Intervention Group 6 Basic feed + nutritional fortification formula F Intervention Group 7 Basic feed + nutritional fortification formula G Intervention Group 8 Basic feed + nutritional fortification formula H Intervention Group 9 Basic feed + nutritional fortification formula I Intervention Group 10 Basic feed + nutritional fortification formula J Intervention Group 11 Basic feed + nutritional fortification formula K Intervention Group 12 Basic feed + nutritional fortification formula L Intervention Group 13 Basic feed + nutritional fortification formula M Comparison with intervention group 14 Basic feed + nutritional fortification formula N Comparison with intervention group 15 Basic feed + nutritional fortification formula O Comparison with intervention group 16 Basic feed + nutritional fortification formula P Comparison with intervention group 17 Basic feed + nutritional fortification formula Q Comparison with intervention group 18 Basic feed + nutritional fortification formula R Comparison with intervention group 19 Basic feed + nutritional fortification formula S Comparison with intervention group 20 Basic feed + nutritional fortification formula T

[0185] In Table 23, AIN-93G_D10012M basic feed and AIN-93G_D10012G growth and development feed were purchased from Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd. AIN-93G_D10012M basic feed meets all the needs of mouse growth, while AIN-93G_D10012G growth and development feed is a feed that has been verified to have the effect of increasing height.

[0186] Calculation of the ratio of customized feed: The age of the corresponding population of 3-week-old (early puberty) C57BL / 6J mice is 4 to 6 years old. According to the Chinese Dietary Nutrient Reference Intake (2023 Edition), the total daily energy requirement of children aged 4 to 6 years is between 1200 and 1600 kcal, and the median is calculated to be 1400 kcal. The energy provided by the recommended daily amount of fortified formula (90 grams) is: 377.46 kcal. The daily energy requirement of C57BL / 6J mice is 15 kcal, so the energy requirement of the fortified formula = 15 kcal × 0.27 = 4.05 kcal, and the energy density of the fortified formula is 419.4 kcal / 100g. Therefore, the amount of fortified formula to be added to each gram of feed = 0.966g / 4g≈0.242g / g feed. The 20 groups of feed in the intervention group were customized with the AIN-93G_D10012M basic feed in the above manner. According to the ratio of the above-mentioned customized feed, the nutritional fortified formula was compounded with the basic feed into the intervention group feed.

[0187] The specific compositions of the nutritional fortification formulas A to T are shown in Examples 1 to 13 and Comparative Examples 1 to 7. The nutritional components thereof differ only in the contents of vitamin D, lysine and basic protein, and the contents of other nutritional components remain basically the same. The specific information of the contents of the three nutritional components is shown in Table 24.

[0188] Table 24

[0189]

[0190] 3. Index detection

[0191] 3.1 Weight and height measurement

[0192] During the experiment, the weight and height of mice in each group were regularly monitored and analyzed every week.

[0193] 3.2 Determination of osteocalcin (BGP) in mouse blood

[0194] The mouse blood samples were centrifuged at 3000 r / min for 15 min and stored at -80°C for later use. The upper serum layer after preparation was taken and the corresponding osteocalcin (BGP) content index was detected using the corresponding kit. The operation steps were strictly carried out according to the kit instructions.

[0195] 3.3 Detection of bone-related indicators

[0196] (1) The obtained mouse femur was fixed in 4% paraformaldehyde solution for more than 48 hours;

[0197] (2) Trim the sample tissue, rinse it with running water for 2 hours, and then fix it with 75% alcohol. Perform microCT scanning on the entire femur to obtain and reconstruct a three-dimensional image of the mouse femur.

[0198] (3) Data reconstruction was performed using NRecon Reconstruction software. DataViewer was used to view 2D slices of the cross section, sagittal plane, and coronal plane of the object, and the 2D slices were rotated and cropped to export new data sets. The same area 2 mm below the femoral growth plate of all mice was selected as the target area of ​​interest. CTAn software was used for data analysis to measure the relative volume of trabeculae (Tb.BV / TV), trabecular thickness (Tb.Th), relative number of trabeculae (Tb.N), trabecular pattern factor (Tb.Pf), and trabecular separation (Tb.Sp) of each group of mice.

[0199] 3.4 Detection methods of hormone indicators

[0200] Corresponding Elisa kits were used to detect various hormone indicators, and the operating steps were strictly carried out in accordance with the kit instructions.

[0201] 4 Experimental Results

[0202] 4.1 Effects on the weight and height of mice

[0203] In order to evaluate the effects of different ratios of nutritional compositions on the weight and length of mice, the changes in weight and length of mice in each group were measured over time, and the results are shown in Tables 25 and 26. Day 0, 4 weeks and 8 weeks were calculated based on the feeding intervention period.

[0204] Table 25

[0205]

[0206] Table 26

[0207]

[0208] Body length is one of the important physiological indicators that reflect the linear growth of mice. The increase in body length directly reflects the growth of non-fat tissue in the body. Therefore, the linear growth of mice can be evaluated by measuring their body length.

[0209] Please refer to Table 26. Compared with the blank control group, the mice in the intervention groups 1 to 13 showed a significant advantage in body length from the 4th week. Moreover, the mice in the intervention groups 2 to 13 also showed a significant advantage in body length compared with the positive control group from the 4th week. This shows that the nutritional composition of the present invention can significantly promote the bone development and linear growth of bones in adolescent mice.

[0210] In contrast, in intervention groups 14 to 16, the nutritional composition only contained one or two of vitamin D, basic protein and lysine. From the results, it can be seen that the height of the mice lags far behind that of intervention groups 1 to 13 from the 4th week, and also lags behind that of the blank control group and the positive control group. This shows that in the nutritional composition of the present invention, the three components of vitamin D, basic protein and lysine are all very critical, and when used in combination, they can play a synergistic role in promoting the height development of adolescent mice, and the lack of any component will lead to a significant reduction in the efficacy.

[0211] In the comparison intervention groups 17-20, the ratio of vitamin D, basic protein and lysine in the nutritional composition was not within the range of (0.005-0.020): (70-200): (1000-3000). From the results, it can be seen that the height of the mice fell behind the intervention groups 1-13 significantly from the 4th week, and also fell behind the blank control group and the positive control group. This shows that in the nutritional composition of the present invention, the three components of vitamin D, basic protein and lysine need to be used in a ratio of (0.005-0.020): (70-200): (1000-3000) to achieve a synergistic effect.

[0212] Please refer to Table 25. After intervention with the nutritional composition, the body weight of the mice during puberty did not increase compared with the blank control group. This indicates that the nutritional composition of the present invention can not only effectively promote the growth of the body length of mice, but also does not increase the possibility of obesity during puberty.

[0213] 4.2 Effects on osteocalcin in mouse serum

[0214] Blood samples were taken from mice in each group at the specified time to test the content of osteocalcin (BGP) in the blood samples. The results are shown in Table 27.

[0215] Table 27

[0216]

[0217] Osteocalcin (Bone Gla Protein, BGP), also known as osteocalcin (OC), is a non-collagenous protein synthesized and secreted by osteoblasts. It is one of the most abundant non-collagenous proteins in bone tissue. Osteocalcin plays an important role in bone growth and metabolism. It can directly reflect the functional state of bone cells and the state of bone formation. Serum osteocalcin level can be used as a specific indicator of osteogenesis. Increased serum osteocalcin level usually indicates accelerated bone formation rate, accelerated bone development, and increased bone matrix mineralization.

[0218] The results in Table 27 show that when vitamin D, basic protein and lysine are used in a nutritional composition in a specific ratio, they show a synergistic effect in increasing the content of osteocalcin in the serum of adolescent mice, thereby significantly promoting bone formation in mice.

[0219] Specifically, in the comparative intervention groups 14 to 20, vitamin D was not added to the nutritional compositions of comparative intervention groups 14 and 15, and only a small amount of vitamin D was added to comparative intervention group 17. From the results, the increment of BGP in comparative intervention groups 14, 15, and 17 was negative, and the increment was smaller than that of other comparative intervention groups. This shows that the content of vitamin D in the nutritional composition is very important. If it is lacking or insufficient, it will have a significant restrictive effect on bone formation.

[0220] Comparing intervention group 5 with control intervention groups 15 and 16, vitamin D was not added to the nutritional composition of control intervention group 15, and basic protein was not added to the nutritional composition of control intervention group 16. From the results, compared with the blank control group, intervention group 5, control intervention groups 15 and 16 increased BGP by 4.94, -1.06 and 0.23 respectively; the increase in BGP in intervention group 5 was greater than the sum of the increases in control intervention groups 15 and 16. This shows that when the three components of vitamin D, basic protein and lysine in the nutritional composition are used together, they can achieve a synergistic effect and maximize the effect of increasing BGP in serum.

[0221] Comparing the results of intervention groups 1-13 with control intervention groups 17-20, the ratio of vitamin D, basic protein and lysine in the nutritional composition is very important. When the three are used in a ratio of (0.005-0.020): (70-200): (1000-3000), the BGP content in serum can be significantly increased, thereby jointly improving bone metabolism and promoting bone health.

[0222] 4.3 Effects on relative trabecular volume (BV / TV), trabecular thickness (Tb.Th), relative trabecular number (Tb.N), trabecular pattern factor (Tb.Pf), and trabecular separation (Tb.Sp) in mice

[0223] Table 28 shows the effect of the nutritional composition on the relative volume of trabecular bone in mice.

[0224] Table 28

[0225]

[0226] Trabecular bone is a tiny structure in bone tissue, mainly composed of osteocytes and bone matrix. It has a highly ordered structure and can withstand pressure and tension from external forces.

[0227] Trabecular bone relative volume (BV / TV), this ratio can reflect the amount of trabecular bone in different samples. An increase in the value indicates that bone synthesis is greater than bone metabolism and bone mass increases.

[0228] The results in Table 28 show that there is a synergistic effect between the nutritional compositions in a specific ratio, which can synergistically promote bone development and health. Compared with the blank control group, the BV / TV trabecular bone relative volume of the intervention groups 1-13 showed an upward trend.

[0229] Specifically, according to the results of intervention group 5 and comparison intervention groups 15 and 16, compared with the blank control group, intervention group 5 and comparison intervention groups 15 and 16 increased BV / TV by 1.41, 0.04 and 0.12 respectively; the increase in BV / TV of intervention group 5 was greater than the sum of the increases of comparison intervention groups 15 and 16.

[0230] The content of vitamin D in the nutritional composition is very important. From the results of comparative intervention groups 14 and 15, it can be seen that when vitamin D is deficient, the restriction effect on bone development is more obvious. From the results of comparative intervention group 17, it can be seen that when the content of vitamin D is insufficient, the increase in bone mass is small.

[0231] Comparing the results of intervention group 5 with those of control intervention groups 16, 18, and 19, the relative volume of trabeculae in intervention group 5 increased by 1.41, 0.12, 0.08, and 0.59, respectively, and the increase in relative volume of trabeculae in intervention group 5 was greater than the sum of the increments of control intervention groups 16, 18, and 19. It can be seen that the ratio between vitamin D, basic protein, and lysine in the nutritional composition is very important. Within a specific ratio range, the three components will produce a synergistic effect, which can significantly increase the relative volume of trabeculae (BV / TV) in a synergistic manner, thereby jointly improving bone metabolism and promoting bone health.

[0232] Tables 29 to 31 respectively show the effects of the nutritional composition on the trabecular thickness (Tb.Th), the relative number of trabeculae (Tb.N), the trabecular pattern factor (Tb.Pf), and the trabecular separation (Tb.Sp) of mice.

[0233] Table 29

[0234]

[0235] Table 30

[0236]

[0237] Table 31

[0238]

[0239] Trabecular thickness (Tb.Th), relative number of trabeculae (Tb.N), trabecular pattern factor (Tb.Pf) and trabecular separation (Tb.Sp) are the main indicators for evaluating trabecular microstructure. There is a certain connection between them and they jointly affect the strength and health of bones.

[0240] Trabecular thickness (Tb.Th): This parameter indicates the average thickness of trabeculae, reflecting the strength and stability of trabeculae. In the case of osteoporosis, trabeculae become thinner, that is, the value of Tb.Th decreases.

[0241] Trabecular number (Tb.N): indicates the number of trabeculae per unit volume. In osteoporosis, the number of trabeculae will decrease, that is, the value of Tb.N will decrease.

[0242] Trabecular pattern factor (Tb.Pf): This is an indicator that describes the connectivity of trabeculae. A decrease in the Tb.Pf value indicates that the structure of cancellous bone has changed from rod-shaped to plate-shaped, so the lower the Tb.Pf value, the better the bone connectivity. In the treatment of osteoporosis, such as the use of bisphosphonates, a significant decrease in Tb.Pf can be observed, indicating that bone connectivity is better preserved.

[0243] Trabecular separation (Tb.Sp): It indicates the average distance between trabeculae. In osteoporosis, the trabecular separation will increase, that is, the value of Tb.Sp will increase.

[0244] The above parameters jointly determine the three-dimensional morphology of trabecular bone, thus affecting the mechanical properties of bone. Through the comprehensive analysis of these parameters, we can have a more comprehensive understanding of the changes in bone quality and the progression of osteoporosis.

[0245] Please refer to Tables 29 to 31. Through Micro-CT observation of bone microstructure parameters, it was found that the results of intervention groups 1 to 13 were better than those of the blank control group, and some results were better than those of the positive control group.

[0246] When a specific ratio of the nutritional composition was used, Tb.Th (trabecular thickness) and Tb.N (relative number of trabeculae) in intervention groups 1 to 13 showed an upward trend compared with the blank control group, indicating that the average thickness of trabeculae in mice increased and bone mass accumulated after intervention. Tb.Pf (trabecular pattern factor) and Tb.Sp (trabecular separation) showed a downward trend, indicating that the spatial structure of trabeculae in mice was good. This result shows that the intake of a specific ratio of the nutritional composition is not only beneficial to the longitudinal growth of bones but also to bone health.

[0247] In terms of the components and dosage used, the results of intervention group 5 and comparative intervention groups 15 and 16 showed that, compared with the blank control group, intervention group 5 and comparative intervention groups 15 and 16 increased Tb.Th by 0.0094, -0.0008 and 0.0001, respectively; the increment of Tb.Th in intervention group 5 was greater than the sum of the increments of comparative intervention groups 15 and 16. Similarly, the increment of Tb.N in intervention group 5, which is the relative number of trabeculae, was greater than the sum of the increments of comparative intervention groups 15 and 16. Intervention group 5 and comparative intervention groups 15 and 16 reduced trabecular separation by 0.07, 0, and 0.05, respectively, and the reduction in trabecular separation in intervention group 5 was greater than the sum of the reductions of comparative intervention groups 15 and 16.

[0248] It can be seen from this that the nutritional composition provided by the present invention is beneficial to the development and health of bones, and when the three components in the nutritional composition are used in a specific ratio, a synergistic effect will be produced between the three components, which can better improve bone morphology and promote bone health.

[0249] 4.3 Effects of hormones related to bone growth and development in mice

[0250] Tables 32 to 35 respectively show the effects of the nutritional compositions on growth hormone (GH), insulin-like growth factor (IGF-1), estrogen (E2), and estrogen receptor (ER-a).

[0251] Table 32

[0252] Group GH (4 weeks) ng / ml GH (8 weeks) ng / ml Blank control group 21.921±10.648 36.722±15.259 Positive control group 42.617±19.389 49.931±22.698 Intervention Group 1 35.538±19.581 38.283±11.808 Intervention Group 2 41.863±12.063 43.564±10.236 Intervention Group 3 40.892±8.436 42.881±6.478 Intervention Group 4 41.037±8.489 43.208±10.532 Intervention Group 5 39.597±9.601 42.658±5.217 Intervention Group 6 38.235±6.678 40.479±5.754 Intervention Group 7 41.712±11.338 43.489±12.567 Intervention Group 8 42.237±14.434 44.189±11.463 Intervention Group 9 42.266±16.969 43.952±23.144 Intervention Group 10 45.244±8.941 55.597±9.601 Intervention Group 11 40.712±7.216 43.184±9.547 Intervention Group 12 36.146±12.223 39.604±6.208 Intervention Group 13 44.985±10.532 50.629±6.743 Comparison with intervention group 14 25.838±6.598 37.237±14.434 Comparison with intervention group 15 26.069±11.506 37.566±10.904 Comparison with intervention group 16 26.722±15.259 37.244±8.941 Comparison with intervention group 17 28.283±11.808 37.838±6.598 Comparison with intervention group 18 30.787±20.012 36.969±21.506 Comparison with intervention group 19 32.377±11.625 37.066±16.969 Comparison with intervention group 20 28.802±8.745 34.847±7.324

[0253] Table 33

[0254] Group IGF1 (4 weeks) ng / ml IGF1 (8 weeks) ng / ml Blank control group 141.951±36.673 126.916±31.215 Positive control group 196.921±83.26 251.37±78.886 Intervention Group 1 174.799±39.239 165.778±71.328 Intervention Group 2 180.271±31.570 174.236±31.478 Intervention Group 3 178.985±42.044 170.547 ±23.324 Intervention Group 4 179.825±42.243 170.436 ±38.423 Intervention Group 5 178.613±48.046 169.536 ±33.375 Intervention Group 6 175.705±34.202 166.725±42.528 Intervention Group 7 177.325±28.589 170.518 ±23.629 Intervention Group 8 181.774±41.117 172.419±42.322 Intervention Group 9 182.073±32.349 170.58±29.442 Intervention Group 10 194.289±25.371 196.296±31.831 Intervention Group 11 178.861±35.256 169.025 ±34.246 Intervention Group 12 176.262±34.451 167.348±61.712 Intervention Group 13 186.315±26.342 180.154±22.513 Comparison with intervention group 14 141.824±41.192 129.137±76.527 Comparison with intervention group 15 142.769±41.967 137.16±67.609 Comparison with intervention group 16 142.916±31.215 133.613±48.046 Comparison with intervention group 17 145.778±34.328 136.704±41.117 Comparison with intervention group 18 144.37±28.886 136.073±50.349 Comparison with intervention group 19 146.536 ±33.375 144.824±41.19 Comparison with intervention group 20 150.721±24.197 146.724±34.376

[0255] Table 34

[0256] Group E2 (4 weeks) ng / ml E2 (8 weeks) ng / ml Blank control group 87.608±8.91 93.083±12.832 Positive control group 90.898±10.32 106.865±13.958 Intervention Group 1 101.324±10.018 99.627±12.494 Intervention Group 2 105.223±9.056 108.536±8.056 Intervention Group 3 103.154±8.326 101.044±9.043 Intervention Group 4 105.228±9.029 101.123±8.424 Intervention Group 5 102.255±9.381 101.348±7.007 Intervention Group 6 101.928±10.025 99.861±11.247 Intervention Group 7 107.789±6.324 104.256±12.538 Intervention Group 8 104.704±9.366 109.218±15.737 Intervention Group 9 104.331±8.732 109.04±18.844 Intervention Group 10 110.961±5.901 115.818±14.294 Intervention Group 11 106.283±8.325 108.251±10.512 Intervention Group 12 102.542±9.451 101.025±9.626 Intervention Group 13 109.022±6.314 112.423±9.223 Comparison with intervention group 14 87.944±11.259 95.036±9.764 Comparison with intervention group 15 88.466±7.385 96.689±7.375 Comparison with intervention group 16 89.087±10.241 96.348±9.257 Comparison with intervention group 17 88.247±8.902 98.218±15.986 Comparison with intervention group 18 89.944±11.259 99.404±12.5424 Comparison with intervention group 19 90.466±7.412 99.818±24.093 Comparison with intervention group 20 92.597±9.628 99.094±12.456

[0257] Table 35

[0258] Group ER-α(4 weeks)ng / ml ER-α(8 weeks)ng / ml Blank control group 1113.218±92.319 1089.939±75.616 Positive control group 1101.656±106.548 1068.454±167.49 Intervention Group 1 1176.437±136.534 1042.946±91.033 Intervention Group 2 1210.125±105.643 1056.231±61.841 Intervention Group 3 1189.258±107.124 1029.447±79.472 Intervention Group 4 1206.326±105.087 1035.753±72.366 Intervention Group 5 1183.763±117.756 1022.421±69.169 Intervention Group 6 1178.664±117.756 1022.421±69.169 Intervention Group 7 1176.861±97.259 1040.362±99.463 Intervention Group 8 1209.167±117.023 1021.859±98.645 Intervention Group 9 1215.855±125.842 1006.095±64.853 Intervention Group 10 1228.672±68.331 1017.457±72.545 Intervention Group 11 1205.846±113.413 105.810±78.495 Intervention Group 12 1179.944±85.028 1035.387±88.648 Intervention Group 13 1220.814±61.424 1007.406±78.146 Comparison with intervention group 14 1103.712±120.928 1084.032±87.469 Comparison with intervention group 15 1113.458±93.835 1083.813±109.739 Comparison with intervention group 16 1103.712±98.224 1076.095±74.487 Comparison with intervention group 17 1114.031±75.547 1075.457±82.635 Comparison with intervention group 18 1117.734±87.568 1070.032±67.410 Comparison with intervention group 19 1123.473±73.264 1068.813±59.748 Comparison with intervention group 20 1140.568±84.573 1045.635±78.963

[0259] In terms of linear growth, the GH-IGF-1 axis is the core axis for regulating growth. The main mechanism is: the hypothalamus secretes growth hormone releasing hormone (GHRH) and somatostatin, which regulate the release of GH from the anterior pituitary. After GH enters the blood, it can be transported to the corresponding target tissues by binding to growth hormone binding protein (GHBP); then, it binds to specific GH receptors (GHR) to stimulate the synthesis and secretion of IGF-1 in tissues such as the liver, kidney and skeletal muscle. Most of the IGF-1 then binds to insulin-like growth factor binding protein-3 (IGFBP-3); finally, it is transported to the target tissues through the blood circulation and binds to insulin-like growth factor receptors (IGFR), activating downstream related signaling pathways, thereby causing chondrocyte proliferation and stimulating bone development to promote growth. Therefore, by measuring the levels of growth hormone (GH), insulin-like growth factor (IGF-1), estrogen (E2) and estrogen receptor (ER-a) in mice, the linear growth of mice can be assessed.

[0260] It can be seen from the data in Tables 32 to 35 that after testing the relevant hormones that regulate bone growth and development, it was found that after 4 weeks of feeding with a specific proportion of the nutritional composition, the levels of GH, IGF-1, E2 and ER-a in the intervention groups 1 to 13 were significantly better than those in the blank control group, which indicates that the nutritional composition of the present invention is beneficial to increasing the levels of multiple hormones related to linear growth, and thus can promote the linear growth of bones in developing mice.

[0261] At the same time, the relevant hormones regulating bone growth and development in intervention groups 1 to 13 were better than those in control intervention groups 14 to 20 in different proportions. This shows that in the nutritional composition of the present invention, when vitamin D, basic protein and lysine are compounded in a certain proportion, the levels of multiple hormones related to linear growth can be better improved.

[0262] Specifically, taking GH as an example, the nutritional composition of the comparative intervention group 14 did not contain vitamin D and basic protein. After 4 weeks of intake of the nutritional composition, the GH level was significantly lower than that of the intervention groups 1 to 13; after 8 weeks of intake, the GH level in the comparative intervention group 14 was slightly lower than that of the intervention group 1, and significantly lower than that of the intervention groups 2 to 13. The nutritional compositions of the comparative intervention groups 15 and 16 did not contain vitamin D and basic protein, respectively. After 4 weeks of intake of the nutritional composition, the GH level was higher than that of the comparative intervention group 14, but still significantly lower than that of the intervention groups 1 to 13; after 8 weeks of intake, the GH level in the comparative intervention groups 15 and 16 was higher than that of the comparative intervention group 14, but still slightly lower than that of the intervention group 1, and significantly lower than that of the intervention groups 2 to 13. This shows that when the nutritional composition contains the three components of vitamin D, basic protein and lysine at the same time, the GH level can be better improved.

[0263] The results of intervention groups 1-13 and control intervention groups 17-20 show that the ratio of vitamin D, basic protein and lysine in the nutritional composition is very critical. When the ratio of vitamin D, basic protein and lysine in the nutritional composition is not within the range of (0.005-0.020): (70-200): (1000-3000), the GH level is significantly lower than that of intervention groups 1-13. It can be seen that a specific ratio of vitamin D, basic protein and lysine in the nutritional composition can produce a synergistic effect in improving GH levels, which can more significantly increase GH levels and promote linear bone growth in adolescence.

[0264] In summary, when vitamin D, basic protein and lysine are used in combination in a certain proportion, the three have a synergistic effect and can synergistically improve at least one of the above-mentioned indicators related to bone health and bone development.

[0265] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A nutritional composition, characterized in that It comprises vitamin D, basic protein and lysine; wherein, in the nutritional composition, the mass ratio of vitamin D, basic protein and lysine is (0.005-0.020): (70-200): (1000-3000).

2. A nutritional composition according to claim 1, characterized in that: The basic protein includes colostrum basic protein and / or milk basic protein.

3. A nutritional composition according to claim 1, characterized in that: In the nutritional composition, the mass ratio of vitamin D, basic protein and lysine is (0.005-0.020): (100-200): (1500-3000).

4. Use of the nutritional composition according to any one of claims 1 to 3 in the preparation of a preparation for improving bone health and / or promoting height development.

5. Use of the nutritional composition according to any one of claims 1 to 3 in the preparation of a preparation for promoting linear bone growth in children and adolescents.

6. Use of the nutritional composition according to any one of claims 1 to 3 in the preparation of a preparation for increasing the relative volume, trabecular thickness and relative number of trabeculae, and reducing the trabecular pattern factor and trabecular separation.

7. Use of the nutritional composition according to any one of claims 1 to 3 in the preparation of a preparation for increasing the levels of growth hormone, insulin-like growth factor, estrogen and estrogen receptor.

8. A formula food, characterized in that: A nutritional composition comprising any one of claims 1 to 3.

9. A formula food according to claim 8, characterized in that: In the formula food, the content of vitamin D is 0.005-0.020 mg / 100 g, the content of basic protein is 70-200 mg / 100 g, and the content of lysine is 1000-3000 mg / 100 g.

10. A formula food according to claim 8, characterized in that: In the formula food, the source of vitamin D includes ergocalciferol and cholecalciferol; the source of lysine includes milk powder, whey protein powder and L-lysine; the whey protein powder includes isolated whey protein powder; or, The formula food includes infant food, children's food, adolescent food and adult food; or, The formula foods include infant formula milk powder, baby complementary foods, nutritional or dietary supplements, children's formula milk powder, children's snacks and health foods for the middle-aged and elderly.

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