Insecticidal composition for ectoparasites of livestock and poultry and application thereof

Abstract

The invention relates to an insecticidal composition, in particular to a parasites insecticidal composition containing flumethrin and application of the parasites insecticidal composition. The composition comprises an active component A, an active component B and the balance of an auxiliary agent, and the weight ratio of the active component A to the active component B is (1: 20)-(20: 1); the active ingredient A is flumethrin; and the active ingredient B is selected from any one of moxidectin, ivermectin and doramectin. According to the binary composition, the pesticide effect and the pesticide effect stability of the composition are improved through the synergistic interaction among the active components, and the binary composition has the characteristics of high efficiency, low toxicity, environment friendliness and the like. The binary composition is suitable for preventing and treating ectoparasites such as ticks, bed bugs, fleas, louses, mites, flies and gadflies in farms.

Description

Technical Field

[0001] The invention relates to an insecticidal composition, in particular to a parasiticidal composition containing bifenthrin and application thereof. Background Art

[0002] Livestock and poultry in farms will have different degrees of external parasites. External parasites will not only suck the nutrition of livestock and poultry, but also cause a variety of chronic and wasting diseases in livestock and poultry, seriously affect the growth, development and reproduction of livestock and poultry, reduce the production performance of livestock and poultry, and cause a large number of deaths of livestock and poultry, which will bring serious economic losses to the breeding industry and have a great negative impact on the development of animal husbandry. Some types will also threaten people's health. For a long time, the drugs used to prevent and control external parasites of livestock and poultry are relatively single, mostly single doses of organophosphorus pesticides or antibiotics. Organophosphorus pesticides are highly toxic and have toxic hazards to livestock and poultry and farmers. Antibiotics are mostly single doses, such as ivermectin, which has a serious problem of drug resistance.

[0003] Flumethrin belongs to the pyrethroid pesticides. It works by interfering with the sodium ion channels of the pest's nervous system, thereby affecting the pest's nervous system function. Flumethrin has high activity and stable chemical properties. It is mainly used for the prevention and control of parasites outside the body of poultry and livestock, and has the activity of inhibiting adult egg laying and egg hatching. It has an unusual toxicity to the Malchi strain of the microscopic cattle tick, which is 50 times more toxic than deltamethrin. The development of flumethrin products has extremely positive significance for the development of my country's animal husbandry and the improvement of the prevention and control of animal diseases and insects. Summary of the invention

[0004] One of the purposes of the present invention is to provide an insecticidal composition for livestock and poultry ectoparasites, which has good insecticidal effect, low toxicity and low irritation, significant synergistic effect after compounding, and can delay the development of drug resistance of livestock and poultry ectoparasites, and its application.

[0005] To achieve the above purpose, the present invention adopts the technical solution as follows:

[0006] A livestock and poultry external parasite killing composition, the composition comprises an active ingredient A and an active ingredient B, and the weight ratio of the active ingredient A to the active ingredient B is 1:20 to 20:1;

[0007] The active ingredient A is flumethrin;

[0008] The active ingredient B is selected from any one of moxidectin, ivermectin and doramectin.

[0009] The weight ratio of active ingredient A to active ingredient B in the composition is 1:5 to 5:1.

[0010] An application of the livestock and poultry external parasite killing composition: application of the livestock and poultry external parasite killing composition in preventing and controlling external parasites.

[0011] The external parasites of livestock and poultry are one or more of ticks, bed bugs, fleas, lice, mites, flies and horseflies.

[0012] An insecticide for external parasites of livestock and poultry, the preparation comprises active ingredients and auxiliary materials, wherein the active ingredient is the composition, which accounts for 2-80% of the preparation mass.

[0013] The auxiliary materials are one or more of emulsifier, antioxidant, antifreeze agent, preservative, defoamer, solvent, penetrant and water.

[0014] The preparation is in the form of emulsifiable concentrate, aqueous emulsion, microemulsion, pour-on or soluble liquid.

[0015] When the dosage form is a pour-on agent, the solvent is ethylene glycol phenyl ether.

[0016] The above-mentioned preparations can be prepared according to the requirements in accordance with the technical methods known in the art.

[0017] When the dosage form is emulsifiable concentrate, it comprises the following raw materials in weight percentage: 1% to 20% of flumethrin, 1% to 20% of active ingredient B, 7% to 20% of emulsifier, and the solvent is supplemented to 100%.

[0018] When the dosage form is an aqueous emulsion, it comprises the following raw materials in weight percentage: 1% to 20% of flumethrin, 1% to 20% of active ingredient B, 5% to 30% of emulsifier, 10% to 30% of solvent, 1% to 5% of antifreeze agent, 0.1% to 0.5% of defoamer, and water is added to 100%.

[0019] When the dosage form is a microemulsion, it comprises the following raw materials in weight percentage: 1% to 20% of flumethrin, 1% to 20% of active ingredient B, 10% to 40% of emulsifier, 30% to 50% of solvent, and water to 100%.

[0020] When the dosage form is a soluble liquid, it comprises the following raw materials in weight percentage: 1% to 20% of flumethrin, 1% to 20% of active ingredient B, 5% to 20% of emulsifier, and the solvent is supplemented to 100%.

[0021] When the dosage form is a pour-on agent, it comprises the following raw materials in weight percentage: 0.2% to 0.5% of flumethrin, 0.15% to 0.5% of active ingredient B, 0.5% to 1% of antioxidant, 5% to 15% of penetrant, and the solvent is supplemented to 100%.

[0022] In the above technical solution, the antioxidant is selected from: any one of BHT and BHA or a mixture of the two in any proportion.

[0023] In the above technical solution, the penetrant is selected from: any one of isopropyl myristate and tricaprylin or a mixture of the two in any proportion.

[0024] In the above technical scheme, the emulsifier is selected from: any one of calcium dodecylbenzene sulfonate, dodecylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, non-ionic hydroxy polyethylene oxide block copolymer, castor oil polyoxyethylene ether, polyoxyethylene ether formaldehyde condensate sulfate, and fatty alcohol polyoxyethylene ether, or a mixture of two or more in any proportion.

[0025] In the above technical solution, the defoaming agent is selected from: any one of silicone compounds, silicone oil, lauric acid, and stearic acid, or a mixture of two or more of them in any proportion.

[0026] In the above technical solution, the solvent is selected from: any one of isopropyl myristate, tributyl citrate, acetyl tributyl citrate, diisopropyl adipate, ethylene glycol phenyl ether, N,N-dimethyl decanoyl amide, aromatic solvent oil, dodecylbenzene, N-methyl pyrrolidone, or a mixture of two or more in any proportion. Among them, the pour-on agent uses ethylene glycol phenyl ether as the solvent, which greatly increases the spreadability of the drug solution on the animal surface and improves the efficacy.

[0027] The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, glycerin and urea.

[0028] The present invention also provides an application of the compound insecticide composition in the field of preventing and controlling external parasites of livestock and poultry.

[0029] In the above technical solution, the external parasites include ticks, bed bugs, fleas, lice, mites, flies and horseflies, etc.

[0030] In the above technical scheme, when the compound insecticidal composition is used to prevent and treat external parasites of livestock and poultry, the insecticidal composition is diluted 50-300 times with water and sprayed on the body surface of livestock and poultry and inside the farm, so that the parasites on the body surface of livestock and poultry and the surrounding space are exposed to the agent to achieve a lethal effect. Its applicable dosage forms are emulsifiable concentrates, water emulsions, microemulsions and soluble liquids.

[0031] In the above technical scheme, when the compound insecticidal composition is used to prevent and treat external parasites of livestock and poultry, the insecticidal composition is directly poured along the back of the animal to kill the surface parasites through transdermal absorption. The applicable dosage form is a pour-on agent.

[0032] Compared with the existing technology, it has the following characteristics:

[0033] The ectoparasiticidal composition of the present invention has the characteristics of high efficiency, low toxicity, and environmental friendliness, and is mainly used to solve the resistance problem of traditional ectoparasiticidal drugs. Through experiments, it was found that the insecticidal composition compounded with flumethrin can not only solve the resistance problem, but also has a significantly improved lethal effect on ectoparasites compared with existing ectoparasiticidal products. DETAILED DESCRIPTION

[0034] The specific implementation methods of the technical solution of the present invention are described in detail below, but the present invention is not limited to the following descriptions.

[0035] The composition of the present invention, flumethrin, is compounded with any one of moxidectin, ivermectin or doramectin, and the lethality to the target is greatly improved after compounding, thereby greatly improving the killing effect on the target. The compound composition of the present invention has the characteristics of high efficiency, low toxicity and low stimulation, and is very suitable for the control of external parasites of livestock and poultry. Compared with traditional external parasite control drugs, it has a significant improvement in high efficiency, low toxicity, rapid effect and resistance management.

[0036] Verification test 1: Co-toxicity coefficient of flumethrin and active ingredient B to fleas

[0037] Test subjects: Fleas (laboratory-raised);

[0038] Test method: Refer to the Indoor Bioassay Test Guidelines for Pesticides Part 1: Contact Activity Test for Insecticides, and use the filter paper film method NY / T 1154.8-2007 to conduct activity test on fleas.

[0039] Data statistics: The co-toxicity coefficient method was used to evaluate the activity of the combination of the two against fleas. Then the toxicity regression equation was calculated using DPS statistical software to obtain the LD50 value of each drug, and then the toxicity index and co-toxicity coefficient of the agent were calculated according to the Sun Yunpei method. A co-toxicity coefficient greater than 120 indicates a synergistic effect, a co-toxicity coefficient between 80-120 indicates an additive effect, and a co-toxicity coefficient less than 80 indicates an antagonistic effect. The test results are shown in the following table:

[0040] Table 1 Determination of the co-toxicity coefficient of flumethrin and ivermectin (for fleas)

[0041]

[0042]

[0043] As shown in Table 1, when the ratio of flumethrin to ivermectin was 1:20-20:1, the co-toxicity coefficient against fleas was greater than 120, and there was a synergistic effect. Especially when the ratio was 1:5-5:1, the co-toxicity coefficient exceeded 140, and the synergistic effect was obvious.

[0044] Table 2 Determination of the co-toxicity coefficient of flumethrin and doramectin (for fleas)

[0045]

[0046] As shown in Table 2, when the ratio of flumethrin to doramectin was 1:20-20:1, the co-toxicity coefficient against fleas was greater than 120, and there was a synergistic effect. Especially when the ratio was 1:5-5:1, the co-toxicity coefficient exceeded 140, and the synergistic effect was obvious.

[0047] Table 3 Determination of the co-toxicity coefficient of flumethrin and moxidectin (for fleas)

[0048]

[0049]

[0050] As shown in Table 3, when the ratio of flumethrin to moxidectin was 1:20-20:1, the co-toxicity coefficient against fleas was greater than 120, showing a synergistic effect. Especially when the ratio was 1:5-5:1, the co-toxicity coefficient exceeded 140, showing a significant synergistic effect.

[0051] The preparation method of the dosage form in each embodiment of the present invention is as follows:

[0052] The preparation method of the emulsifiable concentrate is as follows: weigh bifenthrin, active ingredient B, emulsifier and solvent according to the formula, heat and mix them evenly, and then the emulsifiable concentrate can be prepared.

[0053] The preparation method of the aqueous emulsion is as follows: flumethrin, active ingredient B, emulsifier, and solvent are weighed according to a ratio, heated and dissolved to form an oil phase, water, stabilizer, and antifreeze are weighed according to a ratio to form an aqueous phase, the aqueous phase is slowly added to the oil phase under shearing conditions, and finally a defoaming agent is added, and the shearing is continued for 5 minutes to obtain the aqueous emulsion.

[0054] The preparation method of the microemulsion is as follows: weigh bifenthrin, active ingredient B, emulsifier, solvent and water according to the formula, heat to dissolve and mix evenly to obtain the microemulsion.

[0055] The preparation method of the soluble liquid is as follows: weigh bifenthrin, active ingredient B, emulsifier and solvent according to the formula, heat to fully dissolve and mix evenly, and then the soluble liquid can be prepared.

[0056] The preparation method of the pour-on agent is as follows: weigh bifenthrin, active ingredient B, antioxidant, penetrant and solvent according to the formula, heat and mix them evenly, and then the pour-on agent can be prepared.

[0057] The present invention will be described below in conjunction with specific embodiments:

[0058] Preparation Example 1: 8% flumethrin-ivermectin emulsifiable concentrate

[0059] Flumethrin 6g, ivermectin 2g, dodecylphenol polyoxyethylene ether 4g, calcium dodecylbenzene sulfonate 3g, tributyl citrate 10g, aromatic solvent oil S-150# is added to make up to 100g. Emulsifiable concentrate is prepared according to the above preparation method.

[0060] Formulation Example 2: 15% flumethrin-moxidectin emulsifiable concentrate

[0061] 9g of flumethrin, 6g of moxidectin, 8g of castor oil polyoxyethylene ether, 4g of calcium dodecylbenzenesulfonate, 10g of tributyl citrate, and dodecylbenzene is added to make up to 100g. The emulsifiable concentrate is prepared according to the above preparation method.

[0062] Formulation Example 3: 30% flumethrin-doramectin emulsifiable concentrate

[0063] Flumethrin 10g, doramectin 20g, fatty alcohol polyoxyethylene ether 8g, phenethylphenol polyoxyethylene ether 8g, calcium dodecylbenzene sulfonate 4g, isopropyl myristate 10g, dodecylbenzene is added to make up to 100g. Emulsifiable concentrate is prepared according to the above preparation method.

[0064] Preparation Example 4: 6% flumethrin-moxidectin aqueous emulsion

[0065] Flumethrin 1g, moxidectin 5g, fatty alcohol polyoxyethylene ether 8g, phenethylphenol polyoxyethylene ether 2g, calcium dodecylbenzene sulfonate 4g, dodecylbenzene 20g, glycerol 5g, silicone oil 0.2g, water to make up to 100g. A water emulsion was prepared according to the above preparation method.

[0066] Preparation Example 5: 15% flumethrin-ivermectin aqueous emulsion

[0067] 3g of flumethrin, 12g of ivermectin, 8g of fatty alcohol polyoxyethylene ether, 2g of phenethylphenol polyoxyethylene ether, 2g of polyoxyethylene ether formaldehyde condensate sodium sulfate, 8g of calcium dodecylbenzenesulfonate, 20g of dodecylbenzene, 5g of propylene glycol, 0.2g of silicone oil, and water to make up to 100g. A water emulsion was prepared according to the above preparation method.

[0068] Preparation Example 6: 31% flumethrin-doramectin aqueous emulsion

[0069] Flumethrin 20g, doramectin 1g, fatty alcohol polyoxyethylene ether 8g, phenethylphenol polyoxyethylene ether 2g, calcium dodecylbenzene sulfonate 2g, tributyl citrate 15g, dodecylbenzene 5g, ethylene glycol 5g, stearic acid 0.2g, water to 100g. Prepare an aqueous emulsion according to the above preparation method.

[0070] Formulation Example 7: 8% flumethrin-ivermectin microemulsion

[0071] Flumethrin 1g, ivermectin 7g, fatty acid polyoxyethylene ether 20g, calcium dodecylbenzenesulfonate 5g, N,N-dimethyl decanoic acid 50g, water is added to 100g. A microemulsion is prepared according to the above preparation method.

[0072] Formulation Example 8: 13% flumethrin-doramectin microemulsion

[0073] 3g of flumethrin, 10g of doramectin, 20g of castor oil polyoxyethylene ether, 5g of calcium dodecylbenzenesulfonate, 30g of N,N-dimethyl decanoamide, 15g of acetyl tributyl citrate, and water to make up to 100g. A microemulsion was prepared according to the above preparation method.

[0074] Formulation Example 9: 20% flumethrin-moxidectin microemulsion

[0075] Flumethrin 10g, moxidectin 10g, dodecylphenol polyoxyethylene ether 20g, calcium dodecylbenzene sulfonate 5g, N,N-dimethyl decanoic acid 30g, dodecylbenzene 15g, water is added to 100g. A microemulsion is prepared according to the above preparation method.

[0076] Preparation Example 10: 2% flumethrin-ivermectin soluble liquid

[0077] Flumethrin 1g, ivermectin 1g, nonionic hydroxy polyethylene oxide block copolymer 10g, calcium dodecylbenzenesulfonate 2g, N-methylpyrrolidone is added to make up to 100g. A soluble liquid is prepared according to the above preparation method.

[0078] Formulation Example 11: 20% flumethrin-moxidectin soluble liquid

[0079] 5 g of flumethrin, 15 g of moxidectin, 10 g of dodecylphenol polyoxyethylene ether, 5 g of calcium dodecylbenzenesulfonate, 25 g of diisopropyl adipate, and N-methylpyrrolidone are added to make up to 100 g. A soluble liquid is prepared according to the above preparation method.

[0080] Preparation Example 12: 21% flumethrin-doramectin soluble liquid

[0081] Flumethrin 1g, doramectin 20g, dodecylphenol polyoxyethylene ether 8g, calcium dodecylbenzene sulfonate 2g, nonionic hydroxy polyethylene oxide block copolymer 10g, N,N-dimethyl decanoic acid 10g, N-methyl pyrrolidone is added to make up to 100g. A soluble liquid is prepared according to the above preparation method.

[0082] Formulation Example 13: 0.25% flumethrin-doramectin pour-on

[0083] Flumethrin 0.15 g, doramectin 0.1 g, BHA 0.5 g, isopropyl myristate 10 g, ethylene glycol phenyl ether to make up to 100 g. A pour-on agent was prepared according to the above preparation method.

[0084] Preparation Example 14: 0.5% flumethrin-ivermectin pour-on

[0085] Flumethrin 0.3g, ivermectin 0.2g, BHT 1g, isopropyl myristate 5g, ethylene glycol phenyl ether is added to make up to 100g. A pour-on agent is prepared according to the above preparation method.

[0086] Formulation Example 15: 1% flumethrin-moxidectin pour-on

[0087] Flumethrin 0.5 g, moxidectin 0.5 g, BHT 0.8 g, tricaprylin 10 g, ethylene glycol phenyl ether to make up to 100 g. A pour-on agent was prepared according to the above preparation method.

[0088] The products obtained from the various formulation examples of the present invention are subjected to verification tests.

[0089] Control agent 1: 30% flumethrin EC

[0090] 30g of flumethrin, 8g of fatty alcohol polyoxyethylene ether, 8g of phenethylphenol polyoxyethylene ether, 4g of calcium dodecylbenzene sulfonate, 10g of isopropyl myristate, and dodecylbenzene is added to make up to 100g. Emulsifiable concentrate is prepared according to the above preparation method.

[0091] Control agent 2: 0.5% ivermectin pour-on

[0092] 0.5 g of ivermectin, 1 g of BHT, 5 g of tricaprylin, and ethylene glycol phenyl ether are added to make up to 100 g. A pour-on agent is prepared according to the above preparation method.

[0093] Control drug 3: 20% doramectin soluble solution

[0094] 20g of doramectin, 8g of dodecylphenol polyoxyethylene ether, 2g of calcium dodecylbenzene sulfonate, 10g of nonionic hydroxy polyethylene oxide block copolymer, 10g of N,N-dimethyl decanoic acid amide, and N-methyl pyrrolidone are added to make up to 100g. A soluble liquid is prepared according to the above preparation method.

[0095] Control agent 4: 8% ivermectin EC

[0096] 8g of ivermectin, 4g of dodecylphenol polyoxyethylene ether, 3g of calcium dodecylbenzene sulfonate, 10g of tributyl citrate, and aromatic solvent oil S-150# are added to make up to 100g. The emulsifiable concentrate is prepared according to the above preparation method.

[0097] Control drug 5: 0.5% Moxidectin pour-on (commercially available)

[0098] Verification Test 2: Efficacy Test of Spray for Control of Fleas and Bed Bugs

[0099] Test subjects: Fleas and bed bugs (laboratory-reared)

[0100] Test method: Refer to GB / T13919.1-2009 Indoor efficacy test and evaluation of public health insecticides for pesticide registration, spray test.

[0101] Test agents: Preparation Examples 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 were diluted with water to 800 ppm, and control agents 1, 3, and 4 were diluted with water to 800 ppm.

[0102] Statistics:

[0103] Table 4: Efficacy test of formulation examples 1-9 against mosquitoes and flies

[0104]

[0105]

[0106] As can be seen from Table 4, the KT of the preparation of the present invention against fleas and bed bugs 50 The values ​​were all less than 5 minutes, and the 24-hour mortality rate exceeded 90%, while the KT values ​​of control agents 1, 3, and 4 against fleas and bed bugs were 50 The values ​​were all greater than 5 minutes, and the 24-hour mortality rate was no greater than 90%. The preparation of the present invention had a significant synergistic effect on the knockdown and lethality of fleas and bed bugs.

[0107] Verification test 3: On-site efficacy test of external parasites of cattle in farms

[0108] Test subjects: 40 cattle infected with ticks in a cattle farm (similar in age, size and weight)

[0109] Test method: cattle infected with ticks were randomly divided into 8 groups, each with 5 cattle. One group was a blank control group, and the other 7 groups were given the designated pouring agent and dosage, which was poured once along the back of the cattle. The experiment lasted for 1 month, and samples of cattle parasitic ticks were collected on the 0th, 4th, 7th, 15th, and 21st days. 2 The parasitic ticks on the skin of the subjects were investigated to investigate the effects of different agents on the prevention and control of ticks.

[0110] Test drug: Preparation Examples 13, 14, 15, control drugs 2, 5, the dosage is 1 mL / 10 kg body weight.

[0111] Table 5 In vitro tick infection rate among groups Statistical unit: %

[0112]

[0113] As can be seen from Table 5, the control effect of the preparation of the present invention on cattle external parasite ticks is the best in terms of rapid effect and long-lasting effect. Although the control agent 6 has the same efficacy, its ingredients are organophosphorus pesticides, and its safety to humans and livestock is poor. In summary, the livestock and poultry external parasite insecticide composition containing flumethrin of the present invention has the advantages of high efficiency, rapidity, long-lasting effect and safety.

[0114] The percentages in all the formulas of the present invention are weight percentages, and the processing methods and processes of the various preparations of the composition are all existing technologies, which can be adjusted and changed according to different situations. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A parasiticidal composition for livestock and poultry ectoparasites, characterized in that: The composition comprises active ingredient A and active ingredient B, the balance being auxiliary agents, and the weight ratio of active ingredient A to active ingredient B is 1:20 to 20:1; The active ingredient A is flumethrin; The active ingredient B is selected from any one of moxidectin, ivermectin and doramectin.

2. The insecticidal composition for external parasites of livestock and poultry according to claim 1, characterized in that: The weight ratio of active ingredient A to active ingredient B in the composition is 1:5 to 5:

1.

3. An application of the livestock and poultry external parasite killing composition according to claim 1, characterized in that: The livestock and poultry external parasite killing composition is used in preventing and controlling external parasites.

4. The use of the livestock and poultry external parasite killing composition according to claim 3, characterized in that: The external parasites of livestock and poultry are one or more of ticks, bed bugs, fleas, lice, mites, flies and horseflies.

5. An insecticide for external parasites of livestock and poultry, characterized in that: The preparation comprises active ingredients and auxiliary materials, wherein the active ingredient is the composition according to claim 1, which accounts for 2 to 80% of the mass of the preparation.

6. The livestock and poultry external parasite insecticide according to claim 5, characterized in that: The auxiliary materials are one or more of emulsifier, antioxidant, antifreeze agent, preservative, defoamer, solvent, penetrant and water.

7. The livestock and poultry external parasite insecticide according to claim 5 or 6, characterized in that: The preparation is in the form of emulsifiable concentrate, aqueous emulsion, microemulsion, pour-on or soluble liquid.

8. The livestock and poultry external parasite insecticide according to claim 7, characterized in that: When the dosage form is a pour-on agent, the solvent is ethylene glycol phenyl ether.

Images