Sebum management liquid and preparation method thereof

Abstract

The invention provides a sebum management liquid and a preparation method thereof, and the sebum management liquid comprises the following components by mass: 2.3-4.61% of a composite plant exosome; the content of the nano-micelle is 10.45 percent to 26.2 percent; 5.3% to 18.9% of a plant extract; 2.25% to 3.35% of a conditioning agent; and the balance of water. According to the invention, the exosome derived from fresh plants is used as a nano-scale carrier, and interacts with the nano-micelles, so that more efficient active substance loading capacity and natural targeting property are ensured, and the active substances can be more accurately delivered to specific sebaceous gland cells; through combination of an exosome preparation technology and a nano-micelle technology, grease is disintegrated, excessive secretion of sebum is reduced, and formation of blackheads and acnes is prevented by reducing grease oxidation.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and more particularly, to a sebum management solution and a preparation method thereof. Background Art

[0002] At present, the advanced deep sebum management methods on the market mainly utilize the technology relying on high-voltage radio frequency to form nanomicelles from lecithin and non-ionic surfactants under a high-voltage radio frequency instrument. However, this technology has low technicality, high energy consumption, and uneven distribution of nanomicelles. In addition, this preparation has great irritation to the skin because it achieves the effect by swelling and overflowing sebum, rather than reducing the oil titer to let sebum flow out naturally. During the process of sebum swelling, it may trigger the contraction of the arrector pili muscle, thus weakening its effect. To achieve the same effect, it is necessary to use a solvent to enhance the effect, which will cause strong irritation to the skin. Moreover, nanomicelles lack effective active payload capacity, and even under high-voltage radio frequency conditions, their loading capacity will be damaged, making them perform poorly in the preparation, and lacking specific cell targeting.

[0003] Therefore, there is an urgent need to develop a sebum management solution and a preparation method thereof to reduce the irritation to the skin, enhance the loading capacity of active ingredients, and achieve more precise cell targeting. Summary of the Invention

[0004] The present invention aims to provide a sebum management solution and a preparation method thereof, which can effectively reduce the sebum titer and melt the solidified oil in the sebaceous gland by utilizing the active loading capacity and natural targeting of exosomes, thereby improving the skin management effect.

[0005] To achieve the above object, the present invention provides a sebum management solution, which comprises the following components by mass percentage: composite plant exosomes, 2.3% - 4.61%; nanomicelles, 10.45% - 26.2%; plant extracts, 5.3% - 18.9%; regulators, 2.25% - 3.35%; water, the balance.

[0006] The sebum management solution provided by the present invention comprises composite plant exosomes, nanomicelles, plant extracts, regulators, and water. Among them, the composite plant exosomes are nanoscale vesicles released by cells, having natural targeting ability and the ability to carry bioactive substances. The composite plant exosomes are extracted from specific plant cells for precise targeting and acting on sebaceous gland cells to achieve effective sebum management. The composite plant exosomes improve the delivery efficiency and targeting of active ingredients, and reduce the risks of skin irritation and adverse reactions. The nanomicelles are nanoscale aggregates formed through special processes, capable of effectively encapsulating and carrying active substances. These micelles can form a uniform distribution on the skin surface and penetrate deep into pores to decompose and absorb oils. The nanomicelles enhance the penetration power and oil-dissolving ability of the sebum management solution, contributing to more effectively managing and reducing sebum accumulation. Specific plant extracts are rich in bioactive ingredients such as anti-inflammatory and antioxidant components, which help improve the skin condition and enhance the skin protection barrier. The plant extracts not only have a nursing and protective effect on the skin, but also assist in alleviating skin problems caused by excessive sebum secretion, such as acne, blackheads, etc. The regulators are used to optimize the application feel and stability of the product, ensuring the stability of the product during storage and use and optimizing the user experience. Water is used as a solvent to dissolve and stabilize all the above components to maintain the homogeneity of the entire formulation and the mildness during application.

[0007] The present invention uses exosomes from fresh plant sources to form a nanovesicle carrier, combines the self-assembled nanoscale flexible vesicle technology, and performs secondary treatment through a high-pressure homogenizer to prepare the sebum management solution. First, using the active loading ability and long-distance delivery characteristics of exosomes, as well as the targeted uptake ability for sebaceous gland cells, plants suitable for sebum management are screened out. Then, these screened plants are prepared into a nanosized carrier with appropriate particle size, having low surface tension characteristics compatible with self-assembled nanovesicles, avoiding the disruption of orderliness that may be caused by high-pressure homogenization treatment. After treatment with the high-pressure homogenizer, smaller and more uniform nanodroplets are obtained, which can effectively wet and soften the stratum corneum, thereby enhancing the skin permeability. According to the Laplace formula, the smaller the interfacial tension, the smaller the pressure for sebum to diffuse out from the sebaceous glands at the bottom of the pores. The prepared plant exosome nanocarrier and primary nanovesicles, under the action of the high-pressure homogenizer and with appropriate pressure, such as in combination with a membrane cloth, can quickly penetrate to the sebaceous gland part at the bottom of the pores to capture sebaceous gland cells. Using the surfactant carried to reduce the oil titer, relax the arrector pili muscle, and increase the fluidity of sebum, so that sebum can be freed from the skin surface under the action of the appropriate pressure difference between the sebaceous gland duct and the pores, achieving the effect of deep oil removal. During this oil removal process, the plant active substances help prevent the formation of excessive keratin blockage and blackheads caused by the irritation of the gland duct due to oil oxidation, and can also prevent the occurrence of acne, achieving the purpose of skin beautification.

[0008] In any of the above technical solutions, the plant extract includes: an antibacterial agent, which includes at least one of Melaleuca alternifolia leaf oil and white willow bark extract; an activity inhibitor, which includes at least one of burdock root extract and Equisetum arvense extract; an astringent, which includes at least one of Zanthoxylum piperitum fruit extract and witch hazel extract; a solubilizer, which includes at least one of tea seed extract and jojoba seed oil; an anti-inflammatory agent, which includes at least one of Matricaria chamomilla flower extract, Vanilla planifolia fruit extract, Portulaca oleracea extract, dipotassium glycyrrhizinate, and panthenol; a moisturizer, which includes at least one of honey extract, hydrolyzed lupine protein, Centella asiatica extract, and diethylene glycol monoethyl ether; an adsorbent, which includes Fucus vesiculosus extract; and a fragrance agent, which includes at least one of Matricaria chamomilla flower extract, Lavandula angustifolia flower extract, Salvia officinalis leaf extract, Rosmarinus officinalis leaf extract, and Mentha piperita leaf extract.

[0009] Components such as Melaleuca alternifolia leaf oil and white willow bark extract have natural antibacterial properties, can effectively inhibit the growth of harmful bacteria on the skin surface, reduce skin infections and inflammation, improve skin hygiene, and in addition, can also inhibit the formation of biofilms. Burdock root extract and Equisetum arvense extract can inhibit the activity of sebaceous glands, thereby controlling excessive sebum secretion, helping to control oil production, and reducing skin greasiness and inflammation. Astringents such as Zanthoxylum piperitum fruit extract and witch hazel extract have an astringent effect due to containing tannins, can help tighten skin pores, reduce the accumulation of dirt and oil in pores, improve skin appearance, and reduce the formation of acne and blackheads. Components such as tea seed extract and jojoba seed oil can assist in enhancing the solubility of hardened plugs. Active extracts such as Matricaria chamomilla flower extract, Vanilla planifolia fruit extract, Portulaca oleracea extract, dipotassium glycyrrhizinate, and panthenol have significant anti-inflammatory and analgesic effects, relieve skin redness, itching, and inflammation, and provide a soothing feeling. Components such as honey extract, hydrolyzed lupine protein, Centella asiatica extract, and diethylene glycol monoethyl ether can regulate osmotic pressure to assist penetration, fully moisten and hydrate the stratum corneum to provide long-term moisturization. Fucus vesiculosus extract contains abundant polysaccharides and can adsorb and purify impurities and excess oil on the skin. Natural fragrance components such as Lavandula angustifolia flower extract, Salvia officinalis leaf extract, Rosmarinus officinalis leaf extract, and Mentha piperita leaf extract can impart natural aromas. Through the synergistic effect of integrating multiple plant extracts, the sebum management liquid not only targets sebum management but also provides multiple skin care functions such as anti-inflammation, moisturization, cleansing, and relaxation at the same time.

[0010] In any of the above technical solutions, the regulator includes: a pH regulator, which includes at least one of glutamic acid, sodium glutamate, arginine, histidine, and lysine; and a cell membrane affinity agent, which includes phosphatidylcholine.

[0011] pH regulators are used to adjust the acidity and alkalinity of products and increase the ionization constant of the system, making the products less irritating and more effective. These specific amino acids are selected because they are natural and gentle on the skin, and can effectively regulate the pH value without causing irritation. The pH regulator can ensure the compatibility of the skin product with the skin's pH value, reduce skin irritation, and enhance the skin adaptability and use comfort of the product. Among them, glutamic acid or sodium glutamate can slowly adjust the pH value, which is conducive to the formation of a stable bilayer of nanomicelles; arginine, histidine, and lysine can be combined according to specific needs, which can slowly adjust the pH value and increase the ionization constant pKa.

[0012] Phosphatidylcholine is a component with excellent skin cell membrane affinity, which is used to improve the skin absorption efficiency of sebum management fluid. Its molecular structure enables it to enhance the skin barrier function and help other nutritional components penetrate the skin more effectively. The affinity agent can improve the absorption rate and effect of the active ingredients in the product, enhance the overall efficacy of the product, and maintain and repair the skin barrier at the same time. The addition of a small amount of phosphatidylcholine can increase the affinity and persistent retention with cells.

[0013] The present invention also provides a preparation method of sebum management fluid for preparing the sebum management fluid as described above. The preparation method includes the following steps: S100. Shear-mix the composite plant exosomes, nanomicelles, plant extracts, regulators, and water to obtain a mixture; S200. Perform membrane emulsification treatment on the mixture to obtain a sebum management fluid with uniform droplet size.

[0014] The preparation method for preparing sebum management fluid provided by the present invention, through high-shear mixing technology, uniformly disperses the composite plant exosomes, nanomicelles, plant extracts, and regulators in the water-based medium, which helps to break the original particle aggregates, achieve the micronization and homogenization of the components, and improve the stability and bioavailability of the active ingredients. Further, by using rapid membrane emulsification technology to optimize the droplet size distribution, an emulsified liquid with uniform particle size is generated. By controlling the droplet size, the uniformity and stability of the droplets are ensured, thereby improving the quality and effect of the sebum management fluid.

[0015] In any of the above technical solutions, the preparation method of the composite plant exosomes includes the following steps: S111. Mix and remove impurities from the exosome source and hop extract to obtain a composite plant; S112. Perform plasmolysis and filtration on the composite plant in sequence to obtain a filtrate; S113. Centrifuge the filtrate to obtain a precipitate to obtain the composite plant exosomes; wherein, the exosome source includes at least one of skullcap root extract, mistletoe leaf extract, ginseng root extract, and peppermint leaf extract.

[0016] In step S111, the exosome source is mixed with the hop extract, and unnecessary impurities are removed, while the active ingredients are retained, providing a purer raw material basis for subsequent processing. In step S112, plasmolysis is mainly used to separate the required extracellular components from the composite plant, and filtration further removes larger particles or impurities that cannot be removed by plasmolysis. In step S113, the centrifugation step is used to separate the exosomes suspended in the filtrate from the liquid by centrifugal force, and the precipitate can be collected to obtain a product rich in exosomes of the composite plant. Among them, the extract of Mentha piperita L. is designed to target and relax the arrector pili muscle. The hop extract can reduce the number and size of sebaceous glands.

[0017] In any of the above technical solutions, S111 specifically includes: crushing and mixing the exosome source and the hop extract, soaking them in a treatment solution for impurity removal, and then rinsing with a buffer solution to obtain the composite plant.

[0018] In the crushing and mixing step, the selected exosome source and the hop extract are finely crushed to ensure full mixing of the two. Crushing to an appropriate particle size can increase the contact surface area of the materials, which helps with subsequent soaking and component extraction. The crushed mixture is soaked in a specific treatment solution. The purpose of this step is to release the active ingredients and remove unnecessary impurities through the treatment solution. The selection of the treatment solution is crucial for the effective extraction and purification of the components. The soaked mixture will be rinsed with a buffer solution to remove the residual impurities in the treatment solution and ensure the purity of the final obtained composite plant. The buffer solution can also help adjust the pH value and maintain the stability of the active ingredients in the extract.

[0019] In any of the above technical solutions, S112 specifically includes: putting the composite plant into a cryo-treated hypertonic solution for plasmolysis and primary filtration, then adding a cellulase solution and soaking for 8 - 10 minutes, followed by secondary filtration to obtain the filtrate.

[0020] First, the composite plant is put into a hypertonic solution for cryo-treatment. Freezing can cause the water in the plant cells to crystallize, thereby breaking the cell wall and releasing the cell contents. The hypertonic solution helps absorb the released cell sap, further promoting the separation and fragmentation of the cell wall. The mixture after plasmolysis will be subjected to the first filtration, the purpose of which is to remove larger particles and residual unbroken cells, and retain a relatively pure cell sap. Add a cellulase solution and soak for 8 to 10 minutes. The cellulase can help decompose the cellulose in the plant cell wall and further extract the active ingredients inside the cell. After soaking, the second filtration is carried out. This filtration aims to remove the residual solids after enzyme treatment to obtain a purer filtrate.

[0021] In any of the above technical solutions, S113 specifically includes: subjecting the filtrate to a first centrifugation at a rotation speed of 4000g for 10 min - 15 min, a second centrifugation at a rotation speed of 800g - 1200g for 10 min - 20 min, and a third centrifugation at a rotation speed of 18000g - 20000g for 40 min - 50 min to obtain composite plant exosomes.

[0022] Centrifuge the filtrate at a speed of 4000g for 10 to 15 minutes, mainly to remove larger particles and precipitates in the filtrate, including incompletely broken cell debris and other larger impurities. Then, perform centrifugation at a speed of 800g to 1200g for 10 to 20 minutes. The medium-intensity centrifugation helps to further remove smaller particles and may start to concentrate the plant exosomes. Finally, perform centrifugation at a speed of 18000g to 20000g for 40 to 50 minutes to enrich and separate the plant exosomes to ensure their purity and concentration.

[0023] In any of the above technical solutions, the preparation method of the nano micelles includes the following steps: S121, dissolving and stirring water, polyol, low-chain alkylated polyglycerol surfactant, lecithin, and sterol compounds to obtain primary vesicles; S122, diluting the primary vesicles and then performing emulsification treatment to obtain nano micelles.

[0024] After mixing components such as water, polyol, low-chain alkylated polyglycerol surfactant, lecithin, and sterol compounds, primary vesicles are formed through dissolution and stirring. This step is the basis for forming stable nano micelles, ensuring the stable existence of active ingredients in the aqueous phase. Dilute the obtained primary vesicles to reduce their concentration, and then perform emulsification treatment. The emulsification treatment involves using high-shear emulsification technology and rapid membrane emulsification technology to further reduce the vesicle size and improve their stability. The final product is uniform and stable nano micelles, which can effectively encapsulate and protect the active ingredients. Polyol can effectively dissolve the surfactant components, facilitating the orderly formation of nano micelles. The low-chain alkylated polyglycerol surfactant can reduce the interfacial tension, facilitating the formation of flexible vesicles and effectively reducing the droplet size of oils. These components can coat the oils under extremely low van der Waals forces, increasing hydrophilicity and promoting sufficient hydration and wetting, thus helping to loosen and soften the keratin accumulation. Lecithin combined with monoalkyl-chain polyglycerol surfactant is conducive to the formation of more stable nano micelles under the conditions of a rapid membrane emulsifier. Sterol compounds help to spontaneously form primary nano vesicles, increasing the self-assembly property and having the characteristic of low energy consumption.

[0025] In any of the above technical solutions, the ratio of polyol, lecithin, and sterol compounds is (5 - 20):(0.1 - 0.3):(3 - 6).

[0026] As a dispersing surfactant and a substance to increase the solution stability, polyol plays a role in stabilizing the active ingredient and providing the required solvent environment in the preparation of nano-micelles. As a commonly used natural surfactant, lecithin is mainly responsible for forming and stabilizing the membrane structure of micelles in nano-micelles, which helps to improve the biocompatibility and payload protection performance of micelles. Sterol compounds are mainly used to enhance the structural integrity and permeability of micelles, making the micelles more stable and at the same time helping to maintain the drug release rate in the body.

[0027] After adopting the technical solution of the present invention, the following technical effects can be achieved: (1) Exosomes derived from fresh plants act together with nano-level carriers and nano-micelles, ensuring a more efficient active substance loading capacity and natural targeting property, and thus enabling the active substance to be delivered more accurately to specific sebaceous gland cells; (2) Due to the use of spontaneously formed nano-vesicles and carriers with low surface tension, this method can reduce skin irritation. In contrast, in the traditional method, the high-pressure radio frequency technology may add penetration enhancers due to uneven nano-particle sizes and unstable directions, which may lead to skin discomfort; (3) Through the treatment of a rapid membrane emulsifier, smaller, more uniform and directionally consistent nano-micelle droplets can be generated, which helps the active substance to be evenly distributed and effectively penetrate into the deep layer of the skin; (4) The combination of exosome preparation technology and nano-micelle technology not only breaks down sebum, helps reduce excessive sebum secretion, but also prevents the formation of blackheads and acne by reducing lipid oxidation. Description of the Drawings

[0028] The above and / or additional aspects and advantages of the present invention will become apparent and easy to understand from the description of the embodiments in conjunction with the following drawings, wherein: Figure 1 is the electron microscope image of the composite plant exosome provided in Embodiment 1 of the present invention; Figure 2 is the electron microscope image of the composite plant exosome provided in Embodiment 1 of the present invention; Figure 3 is the Zeta potential diagram of the nano-micelles provided in Embodiment 2 of the present invention; Figure 4 is the surface tension measurement result diagram of the nano-micelles provided in Embodiment 2 of the present invention; Figure 5 is the Raman spectrum diagram of the body skin before using the sebum management liquid in Embodiment 3 of the present invention; Figure 6 is Figure 5 the attribution of the characteristic peaks and their representative components in the Raman spectrum diagram; Figure 7 This is the Raman spectrum of the body skin after using the sebum management liquid in Example 3 of the present invention; Figure 8 This is the distribution of the sebum management liquid at different depths of the body skin in Example 3 of the present invention; Figure 9 This is the graph of the efficacy test results of the sebum management liquid on the human body; Figure 10 This is the graph of the efficacy test results of the sebum management liquid on the human body; Figure 11 This is the graph of the efficacy test results of the sebum management liquid on the human body. Detailed implementation manners

[0029] To make the above objects, features and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] The present invention uses exosomes from fresh plant sources to form a nanovesicle carrier, combines the self-assembled nano-flexible vesicle technology, and performs secondary treatment through a membrane emulsifier to prepare a sebum management liquid. First, using the active loading ability and long-distance delivery characteristics of exosomes, as well as the targeted uptake ability of sebaceous gland cells, plants suitable for sebum management are screened. Then, these screened plants are prepared into appropriate nano-sized carriers with low surface tension characteristics compatible with self-assembled nano-vesicles, avoiding the disruption of order that may be caused by high-pressure radio frequency treatment. After rapid treatment with a membrane emulsifier, smaller and more uniform nano-droplets are obtained, which can effectively wet and soften the stratum corneum, thereby enhancing the skin permeability. According to the Laplace formula, the smaller the interfacial tension, the smaller the pressure for sebum to diffuse out from the sebaceous glands at the bottom of the pores. The prepared plant exosome nano-carriers and primary nano-vesicles can quickly penetrate to the sebaceous gland sites at the bottom of the pores under the action of a membrane emulsifier, in cooperation with an appropriate membrane cloth pressure difference, to capture sebaceous gland cells. Using the carried surfactant to reduce the oil titer, relax the arrector pili muscle, increase the fluidity of sebum, and enable sebum to quickly free out of the skin surface under the action of an appropriate pressure difference between the sebaceous gland duct and the pores, achieving the effect of deep removal of oil. This process of removing oil helps prevent the formation of excessive keratin blockage and blackheads caused by the irritation of gland ducts due to oil oxidation, and can also prevent the occurrence of acne, thereby achieving the purpose of skin beautification.

[0031] Exosomes are phospholipid bilayer membrane vesicles released into the extracellular matrix after the fusion of intracellular multivesicular bodies with the cell membrane, with a diameter ranging from 30 nm to 150 nm. These vesicles have a hydrophilic core and are suitable for loading soluble active substances. Exosomes belong to nanoparticles, carry cell markers on their surface, thus have the ability to overcome biological barriers, and naturally have targeting ability, and can effectively transport various biomolecules such as proteins, lipids, DNA, and RNA. Although exosomes provide various new strategies in clinical applications, in the field of cosmetics, their use as a new type of nano-delivery carrier is still in its infancy. The present invention combines exosome preparation technology and nano-micelle technology to obtain a brand-new superconducting vesicle preparation, which effectively reduces sebum titer and helps reduce the solidified oil in sebaceous glands, thereby improving skin management effects.

[0032] An embodiment of the present invention provides a sebum management liquid, which includes the following components by mass percentage: composite plant exosomes, 2.3% - 4.61%; nano-micelles, 10.45% - 26.2%; plant extracts, 5.3% - 18.9%; regulators, 2.25% - 3.35%; water, the balance.

[0033] The sebum management liquid provided by the present invention includes composite plant exosomes, nano-micelles, plant extracts, regulators, and water. Among them, the composite plant exosomes are nano-scale vesicles released by cells, with natural targeting ability and the ability to carry bioactive substances. The composite plant exosomes are extracted from specific plant cells and are used to precisely locate and act on sebaceous gland cells to achieve effective sebum management. The composite plant exosomes improve the delivery efficiency and targeting of active ingredients, and reduce the risk of skin irritation and adverse reactions. Nano-micelles are nano-scale aggregates formed by special processes, which can effectively encapsulate and carry active substances. These micelles can form a uniform distribution on the skin surface and penetrate deep into pores to decompose and absorb oil. Nano-micelles enhance the penetration and oil dissolution ability of the sebum management liquid, and help manage and reduce sebum accumulation more effectively. Specific plant extracts are rich in bioactive ingredients such as anti-inflammatory and antioxidant components, which help improve the skin condition and enhance the skin protection barrier. The plant extracts not only have a nursing and protective effect on the skin, but also assist in alleviating skin problems caused by excessive sebum secretion, such as acne, blackheads, etc. The regulator is used to optimize the application feeling and stability of the product, ensure the stability of the product during storage and use, and optimize the user experience. Water is used as a solvent to dissolve and stabilize all the above components to maintain the homogeneity of the entire formulation and the mildness during application.

[0034] By utilizing the efficient targeting and delivery capabilities of composite plant exosomes and combining the synergistic effects of nanotechnology and natural extracts, the present invention forms a sebum management solution that is highly efficient, gentle, and has specific biological activities. The composition ratio of this formulation is carefully designed to ensure optimal activity expression and skin adaptability.

[0035] In some embodiments of the present application, the plant extracts include: bacteriostatic agents, which include at least one of Melaleuca alternifolia leaf oil and Salix alba bark extract; activity inhibitors, which include at least one of Arctium lappa root extract and Equisetum arvense extract; astringents, which include at least one of Zanthoxylum piperitum fruit extract and Hamamelis virginiana extract; solubilizers, which include at least one of Camellia oleifera seed extract and Jojoba seed oil; anti-inflammatory agents, which include at least one of Matricaria chamomilla flower extract, Vanilla planifolia fruit extract, Portulaca oleracea extract, dipotassium glycyrrhizinate, and panthenol; moisturizers, which include at least one of honey extract, hydrolyzed lupine protein, Centella asiatica extract, and diethylene glycol monoethyl ether; adsorbents, which include Fucus vesiculosus extract; and flavoring agents, which include at least one of Lavandula angustifolia flower extract, Salvia officinalis leaf extract, Rosmarinus officinalis leaf extract, and Mentha piperita leaf extract.

[0036] Ingredients such as Melaleuca alternifolia leaf oil and Salix alba bark extract have natural bacteriostatic properties, can effectively inhibit the growth of harmful bacteria on the skin surface, reduce skin infections and inflammation, improve skin hygiene, and in addition, can also inhibit the formation of biofilms. Arctium lappa root extract and Equisetum arvense extract can inhibit the activity of sebaceous glands, thereby controlling excessive sebum secretion, helping to control oil production, and reducing skin greasiness and inflammation. Astringents such as Zanthoxylum piperitum fruit extract and Hamamelis virginiana extract have an astringent effect due to the presence of tannins, can help tighten skin pores, reduce the accumulation of dirt and oil in pores, improve skin appearance, and reduce the formation of acne and blackheads. Ingredients such as Camellia oleifera seed extract and Jojoba seed oil can assist in enhancing the solubility of hardened plugs. Active extracts such as Matricaria chamomilla flower extract, Vanilla planifolia fruit extract, Portulaca oleracea extract, dipotassium glycyrrhizinate, and panthenol have significant anti-inflammatory and analgesic effects, can reduce skin redness, itching, and inflammation, and provide a soothing sensation. Ingredients such as honey extract, hydrolyzed lupine protein, Centella asiatica extract, and diethylene glycol monoethyl ether can regulate osmotic pressure to assist penetration, fully moisten and hydrate the stratum corneum to provide long-term moisturization. Fucus vesiculosus extract contains abundant polysaccharides and can adsorb and purify impurities and excess oil on the skin. Natural fragrance components such as Lavandula angustifolia flower extract, Salvia officinalis leaf extract, Rosmarinus officinalis leaf extract, and Mentha piperita leaf extract can impart a natural aroma. Through the synergistic effects of integrating multiple plant extracts, the sebum management solution not only targets sebum management but also simultaneously provides multiple skin care functions such as anti-inflammatory, moisturizing, cleansing, and relaxation.

[0037] In some embodiments of the present application, the regulator includes: a pH regulator, which includes at least one of glutamic acid, sodium glutamate, arginine, histidine, and lysine; a cell membrane affinity agent, which includes phosphatidylcholine.

[0038] The pH regulator is used to adjust the acidity and alkalinity of the product and increase the ionization constant required by the system, making the product less irritating and more effective. These specific amino acids are selected because they are natural and gentle on the skin, and can effectively regulate the pH value without causing irritation. The pH regulator can ensure the compatibility of the skin product with the skin pH value, reduce skin irritation, and enhance the skin adaptability and use comfort of the product. Among them, glutamic acid or sodium glutamate can slowly regulate the pH value, which is beneficial to the formation of the bilayer of stable nanomicelles; arginine, histidine, and lysine can be combined according to specific needs, which can slowly regulate the pH value and increase the ionization constant pKa.

[0039] Phosphatidylcholine is a component with excellent skin cell membrane affinity, which is used to improve the skin absorption efficiency of the sebum management liquid. Its molecular structure enables it to enhance the skin barrier function and help other nutrient components penetrate the skin more effectively. The affinity agent can improve the absorption rate and effect of the active ingredients in the product, enhance the overall efficacy of the product, and at the same time maintain and repair the skin barrier. The addition of a small amount of phosphatidylcholine can increase the affinity and persistent retention with cells.

[0040] The embodiment of the present invention also provides a preparation method of a sebum management liquid for preparing the sebum management liquid as described above. The preparation method includes the following steps: S100, shear-mix the composite plant exosomes, nanomicelles, plant extracts, regulator, and water to obtain a mixture; S200, subject the mixture to membrane emulsification treatment to obtain a sebum management liquid with uniform droplet particle size.

[0041] The preparation method provided by the present invention for preparing the sebum management liquid, through high-shear mixing technology, evenly disperses the composite plant exosomes, nanomicelles, plant extracts, and regulator in an aqueous medium, which helps to break the original particle aggregates, achieve the micronization and homogenization of the components, and improve the stability and bioavailability of the active ingredients. Further, by using rapid membrane emulsification technology to optimize the droplet particle size distribution, an emulsion with uniform particle size is generated. By controlling the droplet size, the uniformity, stability, and direction consistency of the droplets are ensured, thereby improving the quality and effect of the sebum management liquid.

[0042] Through an efficient mixing and emulsification process, active ingredients such as plant extracts and composite plant exosomes can better exert their functions of regulating sebum secretion and skin maintenance. The uniform droplet particle size helps to improve the skin absorption rate of the sebum management liquid, enabling the active ingredients to penetrate the skin more effectively, thereby enhancing the actual application effect of the product.

[0043] In some embodiments of the present application, the method for preparing composite plant exosomes comprises the following steps: S111. Mix and remove impurities from the exosome source and hop extract to obtain a composite plant; S112. Subject the composite plant to plasmolysis and filtration in sequence to obtain a filtrate; S113. Centrifuge the filtrate to obtain a precipitate, thereby obtaining composite plant exosomes; wherein, the exosome source comprises at least one of Scutellaria baicalensis root extract, Viscum album leaf extract, Panax ginseng root extract, and Mentha arvensis leaf extract.

[0044] In step S111, the exosome source and hop extract are mixed, and unwanted impurities are removed while retaining the active ingredients, providing a purer raw material basis for subsequent processing. In step S112, plasmolysis is mainly used to separate the required extracellular components from the composite plant, and filtration further removes larger particles or impurities that cannot be removed by plasmolysis. In step S113, the centrifugation step is used to separate the exosomes suspended in the filtrate from the liquid by centrifugal force, and collecting the precipitate yields a product rich in composite plant exosomes.

[0045] Through this series of delicate extraction steps, highly pure exosomes can be effectively extracted from the composite plant. The precise extraction method helps to maintain the biological activity of the plant exosomes, making their effects in the product more remarkable. While improving the yield and purity, this preparation method also takes into account the operational convenience and cost efficiency, thus making the entire preparation process more economical and efficient.

[0046] It should be noted that as one of the sources of composite plant exosome vesicles, the particle size of the exosome source needs to be controlled below 90 nm. Among them, Mentha arvensis leaf extract is designed to target and relax the arrector pili muscle. Hop extract can reduce the number and size of sebaceous glands.

[0047] In some embodiments of the present application, S111 specifically comprises: crushing and mixing the exosome source and hop extract, soaking them in a treatment solution to remove impurities, and then rinsing them with a buffer solution to obtain a composite plant.

[0048] In the crushing and mixing step, the selected exosome source material and hop extract are finely crushed to ensure thorough mixing of the two. Crushing to an appropriate particle size can increase the contact surface area of the material, facilitating subsequent soaking and component extraction. The crushed mixture is soaked in a specific treatment solution. The purpose of this step is to release the active ingredients and remove unnecessary impurities through the treatment solution. The selection of the treatment solution is crucial for the effective extraction and purification of the components. The soaked mixture will be rinsed with a buffer solution to remove residual impurities in the treatment solution and ensure the purity of the final composite plant obtained. The buffer solution can also help regulate the pH value and maintain the stability of the active ingredients in the extract.

[0049] Crushing and mixing increases the contact area of the material, facilitating better action of the soaking and treatment solution on the raw materials, thereby improving the extraction efficiency of the active ingredients. Through the dual cleaning process of the treatment solution and the buffer solution, unnecessary impurities are effectively removed, and finally a composite plant exosome with higher purity is obtained. Rinsing with the buffer solution can effectively control the pH value of the product, avoiding extreme changes in acidity and alkalinity from damaging the active ingredients, thereby maintaining their biological activity.

[0050] In some embodiments of the present application, S112 specifically includes: placing the composite plant in a cryo-treated hypertonic solution for plasmolysis and primary filtration, then adding a cellulase solution and soaking for 8 min - 10 min followed by secondary filtration to obtain a filtrate.

[0051] First, the composite plant is placed in a hypertonic solution for cryo-treatment. Freezing can cause the water in the plant cells to crystallize, thereby breaking the cell wall and releasing the cell contents. The hypertonic solution helps absorb the released cell sap, further promoting the separation and fragmentation of the cell wall. The mixture after plasmolysis will be subjected to the first filtration, the purpose of which is to remove larger particles and residues of unbroken cells and retain a relatively pure cell sap. The cellulase solution is added and soaked for 8 to 10 minutes. Cellulase can help decompose the cellulose in the plant cell wall and further extract the active ingredients inside the cell. After soaking, the second filtration is carried out. This filtration aims to remove the residual solids after enzyme treatment and obtain a purer filtrate.

[0052] Through cryo-treatment and the application of cellulase, the plant cell wall is effectively broken, significantly increasing the release rate of the target active ingredients. The dual filtration process ensures the removal of larger particles and cell debris, guaranteeing the purity and quality of the final filtrate. Using this method can complete the processing from raw materials to purified components in a shorter time, reducing production costs and improving production efficiency at the same time.

[0053] In some embodiments of the present application, S113 specifically includes: subjecting the filtrate to a first centrifugation at a rotational speed of 4000g for 10 min - 15 min, a second centrifugation at a rotational speed of 800g - 1200g for 10 min - 20 min, and a third centrifugation at a rotational speed of 18000g - 20000g for 40 min - 50 min to obtain composite plant exosomes.

[0054] Centrifuge the filtrate at a rotational speed of 4000g for 10 to 15 minutes, which is mainly used to remove larger particles and precipitates in the filtrate, including incompletely broken cell debris and other larger impurities. Then, perform centrifugation at a rotational speed of 800g to 1200g for 10 to 20 minutes. The medium-strength centrifugation helps to further remove smaller particles and may start to concentrate the plant exosomes. Finally, perform centrifugation at a rotational speed of 18000g to 20000g for 40 to 50 minutes to enrich and isolate the plant exosomes to ensure their purity and concentration.

[0055] Multi-stage centrifugation can effectively layer and extract components of different densities and sizes, especially composite plant exosomes, thereby improving the purification efficiency of specific active ingredients. Each stage of centrifugation targets particles of specific sizes and densities, resulting in composite plant exosomes with high purity. This staged centrifugation process also helps to remove potential harmful impurities and improve the safety and stability of the product.

[0056] In some embodiments of the present application, the preparation method of the nano-micelles includes the following steps: S121, dissolving and stirring water, polyol, low-chain alkylated polyglycerol surfactant, lecithin, and sterol compounds to obtain primary vesicles; S122, diluting the primary vesicles and then performing emulsification treatment to obtain nano-micelles.

[0057] After mixing the components of water, polyol, low-chain alkylated polyglycerol surfactant, lecithin, and sterol compounds, primary vesicles are formed by dissolution and stirring. This step is the basis for forming stable nano-micelles, ensuring that the active ingredients can exist stably in the aqueous phase. Dilute the obtained primary vesicles to reduce their concentration, and then perform emulsification treatment. The emulsification treatment involves using high-shear force technology and rapid membrane emulsification technology to further reduce the vesicle size and improve their stability. The final product is uniform and stable nano-micelles that can effectively encapsulate and protect the active ingredients.

[0058] Through precisely controlled emulsification treatment, the prepared nano - micelles have high stability and uniform, direction - consistent particle sizes, which is crucial for improving the bioavailability of drugs and reducing side effects. The use of lecithin and sterol compounds not only enhances the structural stability of the nano - micelles but also effectively protects the payload from damage by the external environment, especially in the in - vivo environment.

[0059] Preferably, the polyol includes at least one of glycerol and diol. Further preferably, the polyol includes at least one of propylene glycol and dipropylene glycol. The polyol can effectively dissolve the surfactant components, which is beneficial to the orderly formation of nano - micelles.

[0060] Preferably, the structure of the low - chain alkylated polyglycerol surfactant can be selected as a double - chain or a single - alkyl chain. The purpose is to reduce the interfacial tension, which is beneficial to the formation of flexible vesicles and can effectively reduce the titre of oils. These components can coat the oils under extremely low van der Waals forces, increase hydrophilicity and promote sufficient hydration and wetting, thus helping to loosen and soften the keratin accumulation. One or more of polyglyceryl - 10 oleate, polyglyceryl - 3 ricinoleate, and polyglyceryl - 6 laurate can be selected according to the need for vesicle formation. Further, the combination of lecithin and the single - alkyl - chain polyglycerol surfactant is beneficial to the formation of more stable nano - micelles under the conditions of a high - speed membrane emulsifier.

[0061] Preferably, the sterol compound is selected as phytosterols or cholesterol, which helps to spontaneously form primary nano - vesicles, increases the self - assembly property, and has the characteristic of low energy consumption.

[0062] In some embodiments of the present application, the ratio of the polyol, lecithin, and sterol compound is (5 - 20):(0.1 - 0.3):(3 - 6).

[0063] As a dispersing surfactant and a substance to increase the solution stability, the polyol plays a role in stabilizing the active ingredients and providing the required solvent environment in the preparation of nano - micelles. As a commonly used natural surfactant, lecithin is mainly responsible for forming and stabilizing the membrane structure of the micelles in nano - micelles, which helps to improve the biocompatibility and payload protection performance of the micelles. Sterol compounds are mainly used to enhance the structural integrity and permeability of the micelles, making the micelles more stable and at the same time helping to maintain the drug release rate in the body.

[0064] By optimizing the component ratio, the stability of nano - micelles in the in - vitro and in - vivo environments is ensured, non - specific release is reduced, thereby improving the therapeutic effect and reducing side effects. The appropriate use of lecithin enhances the biocompatibility of the micelles, and the addition of sterol compounds further ensures the stable existence of the micelles in a complex biological environment, enabling the drug to reach the target site more effectively.

[0065] For example, the mass percentages of the components in the sebum management liquid are as follows: Scutellaria baicalensis root extract or Viscum album var. coloratum leaf extract, 0.8 - 1.5; Mentha piperita leaf extract, 1.5 - 3.0; Panax ginseng root extract, 0.8 - 1.5; Humulus lupulus flower extract, 0.002 - 0.003; polyol, 10 - 25; low-chain alkylated polyglycerol surfactant, 0.1 - 0.5; lecithin, 0.05 - 0.1; sterol compound, 0.3 - 0.6; Melaleuca alternifolia leaf oil or Salix alba bark extract, 0.6 - 2.5; Arctium lappa root extract or Equisetum arvense extract, 0.08 - 0.15; Zanthoxylum piperitum fruit extract or Hamamelis virginiana extract, 0.08 - 0.15; Camellia oleifera seed extract or Simmondsia chinensis seed oil, 1.5 - 3.0; Matricaria chamomilla flower extract or Vanilla planifolia fruit extract or Portulaca oleracea extract or dipotassium glycyrrhizinate or panthenol, 0.08 - 0.6; honey extract or hydrolyzed lupetide or Centella asiatica extract or ethoxydiglycol, 0.08 - 0.5; Fucus vesiculosus extract, 2.0 - 4.0; Lavandula angustifolia flower extract or Salvia officinalis leaf extract or Rosmarinus officinalis leaf extract or Mentha piperita leaf extract, 0.08 - 6.5; glutamic acid or sodium glutamate, 0.15 - 0.25; arginine or histidine or lysine, 1.8 - 2.5; phosphatidylcholine, 0.3 - 0.6; water, the balance.

[0066] Example 1 The embodiment of the present invention provides a preparation method of composite plant exosomes, comprising the following steps: S1. Select fresh Panax ginseng roots, Humulus lupulus flower petals and Mentha piperita leaves, cut them into small pieces and soak them in a baking soda solution for 5 min - 8 min to fully remove impurities on the plant surface, obtaining composite plants; S2. Rinse thoroughly with a cell culture buffer solution, put the composite plants into a cryo-treated highly permeable 30% sucrose solution to fully plasmolyze the plant cells, observe with a microscope after about 8 min, and the protoplasts of the plant cells can be separated from the cell walls and become round; S3. After plasmolysis is completed, take it out and filter out the mixture, then put it into a cellulase solution and shake and soak for 8 min - 10 min, filter with a 0.22 μm filter membrane to remove impurities and large leaf tissues; among them, the filtrate is centrifuged at a speed of 4000 g for 10 min - 15 min at a temperature of 4°C for 2 times, discard the cells at the bottom of the centrifuge, and collect the supernatant; S4. Centrifuge the collected supernatant again at a speed of 800 g - 1200 g for 10 min - 20 min at a temperature of 4°C, and collect the supernatant; S5. Centrifuge the collected supernatant at a speed of 18000 g - 20000 g for 40 min - 50 min at a temperature of 4°C, remove the supernatant, and the obtained precipitate is the composite plant exosomes.

[0067] PEG-800 solution was added to the composite plant exosomes to resuspend the exosomes, and 6 μL was observed under an electron microscope. The electron microscope image is shown in Figures 1-2 shown.

[0068] Example 2 An embodiment of the present invention provides a method for preparing nano micelles, comprising the following steps: S1. Dissolve and stir appropriate amounts of water, polyols, low-chain alkylated polyglycerol surfactants, lecithin and phytosterols to form concentrated primary vesicles spontaneously; wherein the ratio of polyols, lecithin and phytosterols is (5-20): (0.1-0.3): (3-6); S2. The concentrated primary vesicles are diluted for a second time, and then emulsified through an ultrafiltration membrane to obtain uniform nanomicelles.

[0069] The Zeta potential was measured by electrophoretic light scattering method, and the surface tension was measured by hanging drop method. The test results showed that the Zeta potential was -8.729mV and the surface tension was 58.02mN / m, indicating that the composite plant exosomes have diffusion permeability. The Zeta potential and surface tension were measured by three parallel experiments. Figure 3 As shown, it is the Zeta potential diagram of nanomicelles; Figure 4 Shown is the surface tension measurement result of nanomicelles.

[0070] Example 3 An embodiment of the present invention provides a method for preparing a sebum management solution, comprising the following steps: S1. The obtained composite plant exosomes, nano-micelles, plant extracts, regulators and water are first mixed uniformly by high shear to obtain a mixture; S2. The mixture is subjected to membrane emulsification treatment to obtain droplets of uniform particle size, which is the sebum management solution.

[0071] The percutaneous permeability test was carried out by Raman testing on the front area of ​​the human forearm. Before using the product, the human skin was tested by Raman. The laser power used in the experiment was 0.268mW, and the integration time of a single point was 0.5s. Deep Raman imaging was carried out along the XZ direction, using a point-by-point scanning method, with a longitudinal step length of 10μm and a scanning area of ​​20μm×120μm. The entire spectral measurement process was completed within 5min. Figure 5 As shown in the Raman spectrum, the characteristic peaks of human skin appear at 943 cm -1 、1275cm -1 、1455cm -1 、1655cm -1 、2846cm-1 - 2883 cm -1 - 2934 cm -1 and 3226 cm -1 . As Figure 6 shown, they are the peak positions, vibration modes and their main representative components of Raman characteristic peaks in human in - vivo skin.

[0072] Raman spectroscopy was performed on the sebum management liquid to analyze its permeability after being used on in - vivo skin. As Figure 7 shown, the Raman spectrum of the sebum management liquid shows multiple complex characteristic peaks, including 493 cm -1 , 844 cm -1 , 1454 cm -1 , 2903 cm -1 and 3186 - 3450 cm -1 . As Figure 8 shown, through in - depth analysis of the Raman image, the distribution of the alkaline sebum management liquid at different depths in in - vivo skin can be observed. The analysis results show that within 0.5 h, the sebum management liquid penetrates into the stratum corneum; within 2 h, the sebum management liquid breaks through the stratum corneum and enters the viable epidermis; within 4 h and 6 h, the sebum management liquid continuously penetrates in the stratum corneum and the viable epidermis. Therefore, after using the sebum management liquid sample on in - vivo skin, the relative permeabilities at 0.5 h, 1 h, 2 h and 4 h are 1.21%, 4.38%, 6.79% and 8.41% respectively.

[0073] As Figures 9-11 shown, it is the graph of the efficacy test results of the sebum management liquid on the human body.

[0074] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A sebum management solution, characterized in that: The components include the following by mass percentage: composite plant exosomes, 2.3%-4.61%; Nanomicelles, 10.45%-26.2%; Plant extracts, 5.3%-18.9%; Regulators, 2.25%-3.35%; Water, balance.

2. The sebum management solution according to claim 1, characterized in that The plant extracts include: an antibacterial agent, the antibacterial agent comprising at least one of Melaleuca alternifolia leaf oil and Willow bark extract; An inhibitor, the inhibitor comprising at least one of a burdock root extract and an equisetum arvense extract; An astringent, the astringent comprising at least one of a Zanthoxylum bungeanum fruit extract and a Witch Hazel extract; A solubilizing agent, wherein the solubilizing agent comprises at least one of tea seed extract and jojoba seed oil; An anti-inflammatory agent, wherein the anti-inflammatory agent comprises at least one of chamomile flower extract, vanilla flatleaf fruit extract, purslane extract, dipotassium glycyrrhizate, and panthenol; A moisturizer, the moisturizer comprising at least one of honey extract, hydrolyzed lupine protein, Centella asiatica extract, and ethoxydiglycol; an adsorbent, the adsorbent comprising a Fucus vesiculosus extract; The flavoring agent comprises at least one of lavender flower extract, sage leaf extract, rosemary leaf extract and peppermint leaf extract.

3. The sebum management solution according to claim 1, characterized in that: The regulator includes: A pH regulator, wherein the pH regulator comprises at least one of glutamic acid, sodium glutamate, arginine, histidine, and lysine; A cell membrane affinity agent, wherein the cell membrane affinity agent comprises phosphatidylcholine.

4. A method for preparing a sebum management liquid, for preparing the sebum management liquid according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: S100, shear mixing the composite plant exosomes, the nanomicelles, the plant extract, the regulator and the water to obtain a mixture; S200, subjecting the mixture to membrane emulsification treatment to obtain the sebum management liquid having uniform droplet size.

5. The preparation method according to claim 4, characterized in that: The preparation method of the composite plant exosomes comprises the following steps: S111, mixing the exosome source and the hop extract, removing impurities, and obtaining a composite plant; S112, performing plasmolysis and filtration on the composite plant in sequence to obtain a filtrate; S113, centrifuging the filtrate to obtain a precipitate to obtain the composite plant exosomes; Wherein, the exosome source includes at least one of scutellaria root extract, ginkgo mistletoe leaf extract, ginseng root extract, and peppermint leaf extract.

6. The preparation method according to claim 5, characterized in that: The S111 specifically includes: The exosome source and the hop extract are crushed and mixed, and immersed in a treatment liquid to remove impurities, and then rinsed with a buffer solution to obtain the composite plant.

7. The preparation method according to claim 5, characterized in that: The S112 specifically includes: The composite plant is placed in a frozen high osmotic solution for plasmolysis and primary filtration, and then a cellulase solution is added for soaking for 8 minutes to 10 minutes and secondary filtration is performed to obtain the filtrate.

8. The preparation method according to claim 5, characterized in that: The S113 specifically includes: The filtrate is centrifuged once at a speed of 4000g for 10min-15min, twice at a speed of 800g-1200g for 10min-20min, and three times at a speed of 18000g-20000g for 40min-50min to obtain the composite plant exosomes.

9. The preparation method according to claim 4, characterized in that: The method for preparing the nano micelles comprises the following steps: S121, dissolving and stirring water, polyol, low-chain alkylated polyglycerol surfactant, lecithin and sterol compound to obtain primary vesicles; S122, diluting the primary vesicles, and then emulsifying them to obtain the nanomicelles.

10. The preparation method according to claim 9, characterized in that: The ratio of the polyol, the lecithin and the sterol compound is (5-20): (0.1-0.3): (3-6).

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