A method for cultivating a seedling of a sand whip

Abstract

The application provides a sand whip seedling cultivation method, and belongs to the technical field of sand-fixing plant cultivation. The sand whip seedling cultivation method comprises the steps of seed collection, seed dormancy breaking, sowing, seedling raising, seedling hardening, row belt type sand barrier setting, target sand dune pretreatment and transplanting. The application solves the problem of lack of sand whip seedling and artificial cultivation technology on the current mobile sand dune. The seed collection cost in the application is only 4.8-20.0% of the traditional collection cost. According to the sand whip seedling cultivation method, the germination rate is above 95%, the emergence rate is above 85%, the average height of the sand whip on the mobile sand dune is between 30-35 cm in the transplanting year, and the preservation rate can reach above 70%. The sand whip seedling cultivation method is a most key step for the research and development of the sand whip living sand barrier.

Description

Technical Field

[0001] This invention relates to the field of sand-fixing plant cultivation technology, and in particular to a method for cultivating seedlings of sand whips on mobile sand dunes. Background Technology

[0002] Mobile dunes are a type of dune classified according to their mobility, and they are also the most widespread type of dune. They are characterized by their easily shifting location. The features of mobile dunes include sparse surface vegetation, typical dune morphology, and easy movement downwind under wind force. Plants struggle to survive on mobile dunes because the shifting sand particles accumulate and bury the vegetation, eventually causing it to die from dehydration.

[0003] *Psammochloa villosa* is a perennial herbaceous plant belonging to the genus *Psammochloa* in the family Poaceae. It is mainly distributed in shifting sand dunes. Its rhizomes form a network structure beneath the sand surface, creating a community for water and nutrients to sustain the underground parts. This network can extend for several meters, tens of meters, or even the entire shifting sand dune, demonstrating excellent sand-fixing effects. When the rhizomes accumulate enough water and nutrients, under suitable conditions, tillers emerge from the nodes and protrude above the sand surface. As these tillers grow, they gradually form a network on the shifting sand dune and continue to expand, eventually occupying the entire dune. These tillers can reach a height of over 1.8 meters in years with good moisture, and even in years with poor moisture, they can still reach a height of 0.8 cm. This process demonstrates a good windbreak effect. *Psammochloa villosa* primarily reproduces through tillering; seedlings are almost never found in its populations or communities, making it difficult for its windbreak and sand-fixing function to quickly spread to other sand dunes and areas.

[0004] Therefore, the seedling cultivation method of *Rhizophora stylosa* on shifting sand dunes is an effective way to solve this problem. However, wild *Rhizophora stylosa* exhibits seed dormancy. In the articles "Relationship between Seed Dormancy and Germination of *Rhizophora stylosa* on the Ordos Plateau and the Environment" (Huang Zhenying, 2003-07-30) and "Influence of Sand Burial and Seed Size on Seed Germination and Seedling Emergence of *Rhizophora stylosa*" (Huang Zhuyajuan, 2005-09-20), low-temperature stratification was used to promote the germination of new wild *Rhizophora stylosa* seeds collected near the Ordos Sandy Grassland Ecological Research Station of the Chinese Academy of Sciences. The germination rate reached 95%, and the seedling emergence rate was 80.2%. Although this method breaks seed dormancy, the following problems exist: (1) The germination rate and seedling emergence rate are the result of daily watering in flower pots. The seedling emergence time is 30 days, which requires daily watering. Moreover, a large shade net is needed when the temperature is above 30℃, which is not practical on shifting sand dunes. (2) If watering is stopped, what is the survival rate on shifting sand dunes in the same year? (3) Low-temperature stratification of *Ligustrum lucidum* seeds from Minqin, Dengkou, and Gulang resulted in germination and emergence rates of less than 75% or even lower. Furthermore, the cost of collecting wild *Ligustrum lucidum* seeds is 3000 yuan / kg. Therefore, providing a low-cost *Ligustrum lucidum* seed collection method, a universal method for breaking seed dormancy, a high emergence and survival rate, and a practical method for cultivating *Ligustrum lucidum* seedlings on mobile dunes is of great significance for the management of mobile dunes and the restoration of degraded grassland ecosystems. Summary of the Invention

[0005] The purpose of this invention is to provide a method for cultivating *Vaccaria segetalis* seeds and seedlings, thereby solving the problems of high collection costs and lack of seedlings and cultivation techniques for wild *Vaccaria segetalis*.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] This invention provides a practical method for cultivating seedlings of *Sarcandra glabra* on shifting sand dunes, comprising the following steps:

[0008] Step 1: Seed Collection

[0009] (1.1) From early June to late October, cover the plump ears of fruit with nylon net seed bags;

[0010] (1.2) Insert the steel bars next to the tillers and tie them together with the tillers to prevent the tillers from being blown down by the wind after the bags are put on;

[0011] (1.3) From late July to early November, cut the seed bags along with the fruit spikes and collect the seeds. During this process, do not damage the seed bags and steel bars. Recycle them together to reduce the cost of seed collection.

[0012] Step 2: Breaking Seed Dormancy

[0013] (2.1) Remove the seeds from the seed coat and store them in a well-ventilated environment at room temperature when the seed moisture content is less than 14%.

[0014] (2.2) After screening out plump and uniformly sized seeds, disinfect them and rinse them with distilled water 3 to 5 times;

[0015] (2.3) After disinfection, the seeds are soaked in gibberellin at a concentration of 180-300 mg / L for 48-72 hours, and then germinated in a light incubator.

[0016] Step 3: Sowing

[0017] (3.1) The sowing substrate is sand, with fine sand having a particle size of 180-200 μm and coarse sand having a particle size of 300-350 μm. The bottom 5.5-6.5 cm of fine sand and the top 1.8-2.5 cm of coarse sand are used. Before filling the sand, the sand is dried at 100℃ for 48 hours to kill insect eggs and other seeds.

[0018] (3.2) From late April to early July, in a sunroom, when the sand surface temperature reaches above 20℃, sow seeds. First, fill non-woven fabric seedling pots with fine sand, then place the seedling pots on the seedbed. Sow the germinated seeds in the center of the sand surface, and then cover the seeds with 1.8-2.5cm of coarse sand. Water after sowing. After the water has fully penetrated, when there is no water accumulation on the sand surface, cover the sand surface with another 0.25-0.5cm of fine sand to prevent water evaporation. The seedling pots are 8cm wide, with an open end that can be opened to fill with sand, and 10cm long. Each seedling pot contains 8-10 seeds.

[0019] Step 4: Seedling cultivation

[0020] (4.1) Water management: Before the seedlings emerge, water them in the morning and evening to keep the sand surface moist. After the seedlings emerge, reduce the number of waterings, but keep the sand surface from drying out.

[0021] (4.2) Temperature management: When the greenhouse temperature is greater than 35℃, the shade net should be opened in time, and the number of water sprays should be increased in the morning and evening to reduce the temperature of the sand surface;

[0022] Step 5: Seedling drought resistance training

[0023] When the seedlings reach a height of 8-10cm, they are moved to a pre-prepared nursery outside the greenhouse. The nursery is covered with sand to a thickness of 10-20cm. Then, the seedling pots are neatly moved into the nursery. The gaps between the seedling pots are filled with fine sand to the same height as the seedling pots. The gaps between the seedling pots and the nursery ridges are also filled with sand to the same height as the seedling pots. Then, the seedlings are flooded with water.

[0024] Step Six: Setting up row-type sand barriers

[0025] (6.1) Selection of mobile dunes: Select mobile dunes with vegetation cover of less than 2% and gentle slope;

[0026] (6.2) Setting up row-type sand barriers: 10 to 20 meters in front of the windward slope, set up plastic mesh sand barriers with a row spacing of 1.5 meters along a line perpendicular to the wind direction, so that they cover the entire sand dune. The sand barriers should be exposed 30 cm above the sand surface and the remaining 30 cm buried in the sand. The width of the sand barriers should be 60 cm and the length should be determined according to the size of the sand dune.

[0027] Step 7: Pre-treatment of the target sand dunes

[0028] Water the target sand dune, then dig isosceles trapezoidal trenches, wider at the top and narrower at the bottom, then make ridges, and finally cover the trenches and ridges with biodegradable white plastic film, compact it to expel the gas under the film, cover the bottom of the trench with 2-3 cm of fine sand, dig trenches along the sides of the ridges, put the excess plastic film on both sides into the trenches, then cover with sand and compact it, then spread a layer of wet clay 2-3 cm thick and 8-10 cm wide on the compacted sand surface, and make planting holes at the bottom of the trench;

[0029] Step 8: Transplanting

[0030] Transplanting should be done from late June to early August. Water thoroughly with plenty of water 1-2 days before lifting the seedlings. Lift the seedlings on the 3rd-4th day. After the seedlings are transported to their destination, transplant them into holes at the bottom of the trench. Then cover them with sand so that the seedling pots are completely buried in the sand, leaving the seedlings exposed. Seal the holes tightly with clay. Water 1-1.5 kg / pot after transplanting.

[0031] The retention rate was calculated line by line at the end of October, and the retention rate for the year could reach over 70%.

[0032] The seed bags in step one require special specifications, with a width of (25) ～ 30cm) × length (70) ～ 80cm), with rectangular mesh, 5.5 cm in length. ～ 7.0mm, width 1.0mm ～ 1.5mm. The mesh size should be smaller in areas with less rainfall and larger in areas with more rainfall.

[0033] Preferably, seeds are disinfected by soaking them in an HgCl2 solution; the mass concentration of the HgCl2 solution is 0.08-0.12%, and the soaking time is 8-12 minutes.

[0034] Preferably, the light culture time is 14 hours, the temperature is 28-35℃, the light intensity is 2000-3500 Lx, and the dark culture time is 10 hours, the temperature is 20-25℃.

[0035] Preferably, the fine sand has a particle size of 180 - 200 μm and a laying thickness of 5.5 - 6.5 cm; the coarse sand has a particle size of 300 - 350 μm and a laying thickness of 1.8 - 2.5 cm.

[0036] Preferably, the salt content of the sowing substrate is less than 500 mM.

[0037] Preferably, the temperature for seed storage is 13 ～ 30 °C; after sowing, the temperature is maintained at 20 ～ 35 °C.

[0038] Preferably, the irrigation is as follows: after the first irrigation, irrigate once every 4 - 6 days for 2 times; then irrigate once every 7 - 8 days for 2 times; irrigate once every 9 - 10 days for 1 time.

[0039] Preferably, the watering standard for the target dune is: the water poured on the dune surface penetrates to the wet sand layer and is connected to it; the angle between the bottom and the waist of the isosceles trapezoidal ditch is 120°, the bottom width of the ditch is 20 cm, the depth of the ditch is 30 cm, the width of the ridge is 20 cm, two ridges and one ditch form a row, the row spacing is 55 cm, and each row - strip sand barrier only makes one ditch and two ridges. The specific method is watering + trenching + ridging + mulching + edge pressing + edge sealing + hole opening + transplanting + sealing + watering.

[0040] Preferably, during transplantation, the adjacent two - row seedlings are in a "pin" shape, the plant spacing is 1.5 m, and the row spacing is 1.5 m.

[0041] Preferably, the wild Phragmites australis seeds are collected from Minqin, Gulang, Dengkou or Ordos.

[0042] By adopting the above technical solutions, the present invention has the following beneficial effects: The method for cultivating seedling of wild Phragmites australis in the present invention on the mobile dune includes steps such as seed collection, breaking seed dormancy, sowing, seedling management, hardening - off, setting row - strip sand barriers, pre - treatment of the target dune, and transplantation. The seed collection cost of the method of the present invention is 4.8 - 20.0% of the original. According to the method of the present invention, cultivating seedling of wild Phragmites australis on the mobile dune, the germination rate is above 95%, the emergence rate is above 85%. In the year of transplantation, the average height of wild Phragmites australis is between 30 - 35 cm, and the survival rate can reach above 70%. Detailed implementation mode

[0043] The present invention provides a method for cultivating seedling of wild Phragmites australis, including the following steps:

[0044] Step 1: Seed collection

[0045] (1.1) From early June to late October, cover the ear with plump seeds with a nylon bag;

[0046] (1.2) Insert the steel bars next to the tillers and tie them together with the tillers to prevent the tillers from being blown down by the wind after the bags are put on;

[0047] (1.3) From late July to early November, cut the seed bags along with the fruit spikes and collect the seeds. During this process, do not damage the seed bags and steel bars. Recycle them together to reduce the cost of seed collection.

[0048] Step 2: Breaking Seed Dormancy

[0049] (2.1) Remove the seeds from the seed coat and store them in a well-ventilated environment at room temperature when the seed moisture content is less than 14%.

[0050] (2.2) After screening out plump and uniformly sized seeds, disinfect them and rinse them with distilled water 3 to 5 times;

[0051] (2.3) After disinfection, the seeds are soaked in gibberellin at a concentration of 180-300 mg / L for 48-72 hours, and then germinated in a light incubator.

[0052] Step 3: Sowing

[0053] (3.1) The sowing substrate is sand, with fine sand having a particle size of 180-200 μm and coarse sand having a particle size of 300-350 μm. The bottom 5.5-6.5 cm of fine sand and the top 1.8-2.5 cm of coarse sand are used. Before filling the sand, the sand is dried at 100℃ for 48 hours to kill insect eggs and other seeds.

[0054] (3.2) From late April to early July, when the sand surface temperature reaches above 20℃, sow seeds. First, fill the seedling pot with fine sand, then sow the germinated seeds in the center of the sand surface, and then cover the seeds with 1.8-2.5cm of coarse sand. After sowing, water the seeds. After the water has fully penetrated the sand and there is no water accumulation on the sand surface, cover the sand surface with another 0.25-0.5cm of fine sand to prevent water evaporation. The seedling pot is 8cm wide with an open end that can be opened to fill with sand. It is 10cm long and contains 8-10 seeds per seedling pot.

[0055] Step 4: Seedling cultivation

[0056] (4.1) Water management: Before the seedlings emerge, water them in the morning and evening to keep the sand surface moist. After the seedlings emerge, reduce the number of waterings, but keep the sand surface from drying out.

[0057] (4.2) Temperature management: When the greenhouse temperature is greater than 35℃, the shade net should be opened in time, and the number of water sprays should be increased in the morning and evening to reduce the temperature of the sand surface;

[0058] Step 5: Seedling drought resistance training

[0059] When the seedlings reach a height of 8-10cm, they are moved to a pre-prepared nursery outside the greenhouse. The nursery is covered with sand to a thickness of 10-20cm. Then, the seedling pots are neatly moved into the nursery. The gaps between the seedling pots are filled with fine sand to the same height as the seedling pots. The gaps between the seedling pots and the nursery ridges are also filled with sand to the same height as the seedling pots. Then, the seedlings are flooded with water.

[0060] Step Six: Setting up row-type sand barriers

[0061] (6.1) Selection of mobile dunes: Select mobile dunes with vegetation cover of less than 2% and gentle slope;

[0062] (6.2) Setting up row-type sand barriers: 10 to 20 meters in front of the windward slope, set up plastic mesh sand barriers with a row spacing of 1.5 meters along a line perpendicular to the wind direction, so that they cover the entire sand dune. The sand barriers should be exposed 30 cm above the sand surface and the remaining 30 cm buried in the sand. The width of the sand barriers should be 60 cm and the length should be determined according to the size of the sand dune.

[0063] Step 7: Pre-treatment of the target sand dunes

[0064] Water the target sand dune, then dig isosceles trapezoidal trenches, wider at the top and narrower at the bottom, then make ridges, and finally cover the trenches and ridges with biodegradable white plastic film, compact it to expel the gas under the film, cover the bottom of the trench with 3-5cm of fine sand, dig trenches along the sides of the ridges, put the excess plastic film on both sides into the trenches, then cover with sand and compact it, then spread a layer of wet clay 2-3cm thick and 8-10cm wide on the compacted sand surface, and make planting holes at the bottom of the trench;

[0065] Step 8: Transplanting

[0066] Transplanting should be done from late June to early August. Water thoroughly with plenty of water 1-2 days before lifting the seedlings. Lift the seedlings on the 3rd-4th day. After the seedlings are transported to their destination, transplant them into holes at the bottom of the trench. Then cover them with sand so that the seedling pots are completely buried in the sand, leaving the seedlings exposed. Seal the holes tightly with clay. Water 1-1.5 kg / pot after transplanting.

[0067] The retention rate was calculated line by line at the end of October, and the retention rate for the year could reach over 70%.

[0068] Sand whip is a wild plant found on shifting sand dunes, and the seed maturity time is related to the rainfall in the region. In areas with low rainfall, the seeds are harvested earlier, while in areas with high rainfall, the seeds are harvested later. Generally, it is from mid-June to early November each year. In Minqin, it is from mid-June to early July; in Gulang, it is from mid-July; in Dengkou, it is from mid-August to early September; and in Ordos, it is early November.

[0069] This invention involves covering the tillers containing fruit spikes with a nylon net seed bag measuring 30×80cm with a mesh length of 6mm and a width of 1.2mm 7–14 days before the wild sand whip seeds fall off. Once the wild sand whip seeds mature, the bag along with the fruit spikes is cut off, and the wild sand whip seeds are collected. The seeds are stored when the moisture content is ≤14% at a temperature of 13–30℃.

[0070] Before germination, the seeds are disinfected and then soaked in a gibberellin solution. After soaking, germination is carried out. The concentration of the gibberellin solution is 180–300 mg / L, more preferably 200–280 mg / L, and even more preferably 200 mg / L. After soaking, germination is carried out. During germination, the light culture time is 14 h / day, the light culture temperature is 28–35℃, more preferably 29–31℃, and even more preferably 30℃, the light intensity is 2000–3500 Lx, the dark culture time is 10 h, the temperature is 20–25℃, more preferably 19–24℃, and even more preferably 23℃, and the germination time is 7–14 days. After germination, the seeds are sown from late April to early July. The seeds are sown on a surface of fine sand, then covered with coarse sand. The fine sand has a particle size of 180–200 μm and a thickness of 5.5–6.5 cm, more preferably 6 cm. The coarse sand has a particle size of 300–350 μm and a thickness of 1.8–2.5 cm, more preferably 1.8–2.2 cm, more preferably 2 cm. After thorough watering, another layer of fine sand is added, with a thickness of 0.25–0.5 cm, more preferably 0.5 cm. The purpose of covering with fine sand is to prevent moisture evaporation. Preferably, the seeds are sown in seedling pots and then cultivated in a greenhouse at a temperature of 15–35°C. When the greenhouse temperature exceeds 35°C, the shade netting should be opened promptly, and watering frequency should be increased in the morning and evening to lower the sand surface temperature. Eight to ten days after sowing, the seedlings will reach a height of 8 to 10 cm. At this time, hardening off the seedlings is necessary. They should be moved to a pre-prepared nursery outside the greenhouse. The nursery should be covered with a layer of sand about 10 to 20 cm thick. The seedling pots should then be neatly placed in the nursery, filling the gaps between the pots with fine sand until they are level with the pots. The gaps between the pots and the nursery ridges should also be filled with sand until they are level with the pots. Then, thoroughly irrigate the seedlings. Because sandy areas have high evaporation rates, increasing the moisture content of the sandy soil is beneficial for root development. Well-established roots help the seedlings develop drought resistance. The first watering must be thorough. Afterward, irrigate once every 5 days, then once every 7 days, then once every 10 days. After this first irrigation, the seedlings will reach a height of 15 to 20 cm, and most of the roots will have emerged from the seedling pots, reaching a length of 8 to 10 cm. At this point, they are ready for transplanting (late June to early August).

[0071] Set up row - type sand barriers: Select gentle flowing sand dunes with vegetation coverage less than 2%. Set up sand barriers 10 - 20 m in front of the windward slope along the direction perpendicular to the wind direction. The row spacing of the sand barriers is 1.3 - 1.7 m, further preferably 1.4 - 1.6 m, and more preferably 1.5 m; the width of each sand barrier is 58 - 62 m, further preferably 59 - 61 m, and more preferably 60 m. The sand barrier exposes 30 cm above the sand surface, and the remaining 30 cm is buried in the sand. The sand barrier is preferably a plastic mesh sand barrier.

[0072] Pre - treatment of target sand dunes: Water the sand dunes with sand barriers set up to make the dry sand layer connect with the wet sand layer; then, dig trenches and form ridges. The trenches are made into isosceles trapezoids, wider at the top and narrower at the bottom. The angle between the bottom and the waist of the isosceles trapezoid trench is 120°, the bottom width of the trench is 20 cm, the depth of the trench is 30 cm, and the width of the ridge is 20 cm. Finally, cover the trenches and ridges with a 2.0 - m - wide degradable white plastic film, press it firmly, evacuate the gas under the film, cover 3 - 5 cm of fine sand at the bottom of the trench, dig trenches at the edges of the ridges, put the extra 20 - cm - wide film on both sides into the trenches, and then cover with sand and press firmly to make the sand connect with the plastic mesh sand barrier. Finally, lay a layer of clay with a thickness of 3 - 5 cm on the 15 - cm - wide sand on both sides contacting the sand barrier, and water the clay. Two ridges and one trench form a row, and the row spacing is 55 cm. Each row is carried out according to the steps of watering + digging trenches + forming ridges + covering with film + pressing the edges + sealing the edges + making holes + transplanting + sealing the holes + watering.

[0073] Transplant the seedlings that meet the nursery - out standard. 1 - 2 days before lifting the seedlings, flood - irrigate with a large amount of water to thoroughly moisten the soil. On the 3rd - 4th day, the seedlings can be lifted. During the process of lifting the seedlings, avoid damaging the fine roots outside the nutrient pots. Spray an appropriate amount of water before transportation to avoid excessive water loss and wilting of the seedlings. Make holes at the bottom of the trench at a distance of 1.5 m, with the hole diameter of 10 cm and the depth of 12 cm. After the seedlings are transported to the destination, transplant them into the holes made. Then, cover with sand to completely bury the nutrient pots in the sand, expose the seedlings, and seal the surrounding of the hole tightly with clay. Water after transplantation, 1 - 1.5 kg per pot, and the seedlings in adjacent rows show a "pin" shape. ～ 2d flood - irrigate to thoroughly moisten the soil, and on the 3rd ～ 4d, the seedlings can be lifted. Avoid damaging the fine roots outside the nutrient pots during the process of lifting the seedlings. Spray an appropriate amount of water before transportation to avoid excessive water loss and wilting of the seedlings. Make holes at the bottom of the trench at a distance of 1.5 m, with the hole diameter of 10 cm and the depth of 12 cm. After the seedlings are transported to the destination, transplant them into the holes made. Then, cover with sand to completely bury the nutrient pots in the sand, expose the seedlings, and seal the surrounding of the hole tightly with clay. Water after transplantation, 1 ～ 1.5 kg per pot, and the seedlings in adjacent rows show a "pin" shape.

[0074] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0075] Example 1

[0076] Seed collection: The seeds are collected from Minqin area, where the wild sand whips mature from mid - to late June to early July. One week before the seeds fall off, cover them with a nylon net seed bag with a size of 30×80 cm, a mesh hole length of 5.5 mm, and a width of 1.0 mm.

[0077] Select tillers with long ears of fruit, plump seeds, and robust, disease-free plants for bagging. To prevent the tillers from being blown over by the wind after bagging, cut 8mm steel bars to 2m lengths and straighten them. Insert one end of the steel bar 2-3cm beside the selected tiller, to a depth of 20cm. One week before the seeds fall off, cover the selected ears of fruit and steel bars with nylon mesh seed bags measuring 30×80cm, with a mesh length of 5.5mm and a width of 1.0mm. Seal the bottom of the seed bag with the pull string and tie it tightly to the steel bar.

[0078] Seed storage: In late July, cut the seed bags along with the fruit spikes (be careful not to damage the seed bags for use next year), and let them dry for one week. Remove the seed bags and collect the wild sand whip seeds, then let them dry again. When the seed moisture content is less than 14%, store them in cotton bags at a temperature of 13–30℃. During storage, avoid smoke, rodents, insects, and moisture.

[0079] Breaking seed dormancy: In April, select plump, uniformly sized wild *Vallisneria natans* seeds and soak them in a 0.08% HgCl2 solution for 12 minutes. Remove the seeds and rinse them repeatedly with distilled water five times. Soak the sterilized seeds in a 180 mg / L gibberellin solution for 72 hours, stirring every 4 hours. After soaking, dry the seed surface and rinse them repeatedly with distilled water five times. Then, place them in a light incubator for germination. The daily light duration is 14 hours, the light intensity is 3000 Lx, the temperature for light incubation is 28℃, and the temperature for dark incubation is 20℃. The germination time is 12 days.

[0080] Sowing: Sowing was carried out in late April in a greenhouse. Non-woven fabric seedling pots with a diameter of 8cm and a height of 10cm were used as sowing containers. Sand with a salt content of 386mM was selected as the sowing substrate. Before sowing, the sand was dried at 100℃ for 48 hours and spread in the seedling pots to a thickness of 5.5cm. ～ Prepare 6.5cm of fine sand with a particle size of 180-200μm. Sow 10 pre-germinated seeds on the sand surface in each seedling pot, and cover them with 2.5cm of coarse sand with a particle size of 300-350μm. Water after sowing, using 1kg of water per pot. Once the water has fully soaked in and there is no standing water on the sand surface, cover the sand surface with another layer of fine sand about 0.5cm thick to prevent moisture evaporation.

[0081] Seedling management: Before emergence, water twice daily to keep the sand surface moist. Seedlings will emerge 10 days after sowing. After emergence, reduce watering frequency appropriately, but keep the sand surface moist. Seedlings will reach a height of 8-10cm 20 days after sowing. During the seedling stage, maintain an indoor temperature of 30℃. If the greenhouse temperature exceeds 35℃, open the shade netting promptly and increase watering frequency in the morning and evening to lower the sand surface temperature.

[0082] Drought hardening of seedlings: When the seedling height reaches about 10 cm and there are hairy roots showing out of the nutrient bowl, they can be transplanted to the nursery prepared in advance outside the greenhouse. The thickness of the sand laid in the nursery is about 10 cm. Then, the nutrient bowls are neatly moved into the nursery, and the gaps between the nutrient bowls are filled with fine sand to the same height as the nutrient bowls. The gaps between the nutrient bowls and the nursery ridge are also filled with sand to the same height as the nutrient bowls. Then, flood irrigation is carried out. The first watering is thorough. Then, flood irrigation is carried out once every 5 days. After 2 times, flood irrigation is carried out once every 7 days. After 2 times, irrigation is carried out once every 10 days. After 1 time, at this time, the seedling height can reach 15 - 20 cm, most of the roots have shown out of the nutrient bowl, and the length reaches 8 - 10 cm, and then they can be taken out of the nursery for transplanting.

[0083] Setting up row - strip sand barriers: Select mobile dunes with a vegetation coverage of 1.2% and relatively gentle slopes for seedling transplantation. Set up plastic mesh sand barriers with a row spacing of 1.5 m along the direction perpendicular to the wind direction 10 m in front of the windward slope, so that they cover the entire dune. The sand barriers expose 30 cm above the sand surface, and the remaining 30 cm is buried in the sand. The width of the sand barrier is 60 cm, and the length is 288 m.

[0084] Pretreatment of the target dune: First, water the dune with the sand barrier already set up to make the dry sand layer connect with the wet sand layer. Then, dig trenches and make ridges. The trenches are made into isosceles trapezoids. The angle between the bottom of the isosceles trapezoid and the waist is 120°, the bottom width of the trench is 20 cm, the depth of the trench is 30 cm, and the width of the ridge is 20 cm. Finally, cover the trenches and ridges with a 2.0 - m - wide degradable white plastic film, press it firmly, evacuate the gas under the film, cover 3 cm of fine sand at the bottom of the trench, dig trenches at the edges of the ridge, put the extra 20 - cm - wide film on both sides into the trench, and then cover it with sand and press it firmly to make the sand connect with the plastic mesh sand barrier. Finally, lay a 3 - cm - thick layer of clay on the 15 - cm - wide sand on both sides contacting the sand barrier, and water the clay. One row consists of two ridges and one trench, and the row spacing is 55 cm. Each row is carried out according to the steps of watering + digging trenches + making ridges + covering with film + pressing the edges + sealing the edges + making holes + transplanting + sealing the holes + watering.

[0085] Transplanting is carried out at the end of June. Two days before lifting the seedlings, flood irrigation is carried out thoroughly. On the third day, lift the seedlings, and avoid damaging the hairy roots outside the nutrient bowl when lifting the seedlings. Spray an appropriate amount of water before transportation to avoid excessive water loss and wilting of the seedlings. Make holes at a distance of 1.5 m in advance at the bottom of the trench. The diameter of the hole is 10 cm, and the depth is 12 cm. Transplant the seedlings into the dug holes. Then, cover the seedlings with sand so that the nutrient bowl is completely buried in the sand, and expose the seedlings. Seal the surrounding of the hole tightly with clay. Water after transplantation, 1 kg per bowl. The seedlings in adjacent rows are in a "pin" shape, that is, the seedlings in the second row are planted in the middle position between two seedlings in the corresponding first row. The plant spacing is 1 m, and the row spacing is 1.5 m.

[0086] Example 2

[0087] Seed collection: Seeds were collected in the Gulang area, where wild sand peppers begin to mature in mid-to-late July. One week before the seeds fall, they were covered with nylon mesh seed bags measuring 30×80cm with a mesh length of 6mm and a width of 1.2mm.

[0088] Select tillers with long ears, plump seeds, and robust, disease-free plants for bagging. To prevent the tillers from being blown over by the wind after bagging, cut 8mm steel bars into 1.6–2.0m lengths and straighten them. Insert one end of the steel bar 2–3cm beside the selected tiller, to a depth of 20cm. One week before seed detachment, cover the selected ears and steel bars with 30×80cm nylon mesh seed bags with 6.0mm mesh length and 1.2mm width. Seal the bottom of the seed bag with the pull string and tie it tightly to the steel bar.

[0089] Seed storage: In mid-August, cut the seed bags along with the fruit spikes (be careful not to damage the seed bags for use next year), and let them dry for one week. Remove the seed bags and collect the wild sand whip seeds, then let them dry again. When the seed moisture content is less than 14%, store them in cotton bags at a temperature of 13–30℃. During storage, avoid smoke, rodents, insects, and moisture.

[0090] Breaking seed dormancy: In May, select plump, uniformly sized wild *Vallisneria natans* seeds and soak them in a 0.1% HgCl2 solution for 10 minutes. Remove the seeds and rinse them repeatedly with distilled water 5 times. Soak the sterilized seeds in a 200 mg / L gibberellin solution for 72 hours, stirring every 5 hours. After soaking, dry the seed surface and rinse them repeatedly with distilled water 5 times. Then, place them in a light incubator for germination. The daily light duration is 14 hours, the light intensity is 3500 Lx, the temperature for light incubation is 30℃, and the temperature for dark incubation is 21℃. The germination time is 11 days.

[0091] Sowing: Sowing is carried out in early May in a greenhouse. Use non-woven fabric seedling pots (8cm in diameter, 10cm in height) as sowing containers. Select sand with a salt content of 362mM as the sowing substrate. Before sowing, dry the sand at 100℃ for 48 hours. Spread a 6cm layer of fine sand (180-200μm particle size) in the seedling pot, then sow 10 pre-germinated seeds on the sand surface. Cover the seeds with 2cm of coarse sand (300-350μm particle size). Water after sowing, using 1kg of water per pot. Once the water has fully penetrated and there is no standing water on the sand surface, cover the sand surface with another 0.5cm layer of fine sand to prevent moisture evaporation.

[0092] Seedling management: Before emergence, water twice daily to keep the sand surface moist. Seedlings will emerge 8 days after sowing. After emergence, reduce watering frequency appropriately, but keep the sand surface moist. 17 days after sowing, seedlings will reach a height of 8-10cm. During the seedling stage, maintain an indoor temperature of 32℃. If the greenhouse temperature exceeds 35℃, open the shade netting promptly and increase the frequency of water spraying in the morning and evening to lower the sand surface temperature.

[0093] Seedling drought resistance training: When the seedlings reach a height of about 10cm and have fine roots protruding from the nutrient pots, they can be moved to a pre-prepared nursery outside the greenhouse. The nursery should be covered with sand about 10cm thick, and the nutrient pots should be neatly placed inside. The gaps between the nutrient pots should be filled with fine sand until they are level with the pots, and the gaps between the nutrient pots and the nursery ridges should also be filled with sand until they are level with the pots. Then, water thoroughly with a large amount of water. After that, water thoroughly every 5 days, then every 7 days after two waterings, then every 10 days after two more waterings. After one watering, the seedlings will be 15-20cm tall, and most of the roots will have protruded from the nutrient pots, reaching a length of 8-10cm. At this point, they are ready for transplanting.

[0094] Setting up row-type sand barriers: Select gently sloping sand dunes with 1.6% vegetation cover for seedling transplantation. At the windward slope, 15m in front, set up plastic mesh sand barriers with a row spacing of 1.5m perpendicular to the wind direction, covering the entire dune. 30cm of the barriers should protrude above the sand surface, with the remaining 30cm buried. The barriers should be 60cm wide and 197m long.

[0095] Pre-treatment of the target sand dunes: First, water the sand dunes with sand barriers in place to ensure contact between the dry and wet sand layers. Then, dig trenches and create ridges. The trenches should be isosceles trapezoids with a 120° angle between the bottom and sides, a 20cm wide bottom, and a 30cm deep depth. The ridges should be 20cm wide. Finally, cover the trenches and ridges with a 2.0m wide biodegradable white plastic film, pressing it firmly to expel any air trapped beneath. Cover the bottom of the trenches with 4cm of fine sand. Dig trenches along the ridge edges, placing the excess 20cm of film on both sides into the trenches, then cover with sand and press firmly to ensure the sand connects to the plastic mesh sand barrier. Finally, spread a 4cm thick layer of clay on each 15cm layer of sand in contact with the sand barrier on both sides, and water the clay. Two ridges and one trench constitute one row, with a row spacing of 55cm. Each row is treated using the following steps: watering + trenching + ridge making + film covering + edge pressing + edge sealing + hole making + transplanting + sealing + watering.

[0096] Transplanting is carried out in early July. Two days before lifting the seedlings, flood irrigation is used to thoroughly water the seedlings. On the third day, lift the seedlings, and avoid damaging the fine roots outside the nutrient pots when lifting the seedlings. Spray an appropriate amount of water before transportation to prevent the seedlings from wilting due to excessive water loss. Holes are drilled at the bottom of the ditch in advance at a spacing of 1.5 m. The diameter of the hole is 10 cm and the depth is 12 cm. Transplant the seedlings into the dug holes, and then cover them with sand so that the nutrient pots are completely buried in the sand, exposing the seedlings. Seal the surrounding of the hole tightly with clay. Water after transplantation, 1.2 kg per pot. The adjacent two rows of seedlings are in a "pin" shape, that is, the seedlings in the second row are planted in the middle position corresponding to the two seedlings in the first row. The plant spacing is 1.2 m and the row spacing is 1.5 m.

[0097] Example 3

[0098] Seed collection: Seeds are collected from the Dengkou area, where the wild Sabina vulgaris mature from mid-August to early September. One week before the seeds fall off, cover them with a nylon net seed bag with a size of 30×80 cm, a mesh length of 6.5 mm, and a width of 1.2 mm.

[0099] Select tillers with longer ear spikes, plump seeds, and healthy plants without diseases for bagging. To prevent the tillers from being blown down after bagging, cut 8 mm steel bars into 1.6 - 2.0 m (the length of the steel bar depends on the plant height of the tiller, generally the total length of the steel bar is 20 cm higher than the plant height of the tiller) and straighten them. Insert one end of the steel bar 2 - 3 cm beside the selected tiller, with an insertion depth of 20 cm. One week before the seeds fall off, cover the selected ear spike and the steel bar with a nylon net seed bag with a size of 30×80 cm, a mesh length of 6.5 mm, and a width of 1.2 mm. Seal the bottom of the seed bag with the drawstring of the seed bag and tie it tightly to the steel bar.

[0100] Seed storage: In late September, cut off the seed bag together with the ear spike (be careful not to damage the seed bag for use in the coming year), sun-dry for one week, remove the seed bag to collect the wild Sabina vulgaris seeds, and sun-dry after collection. When the water content of the seeds is less than 14%, store them in a cotton cloth bag at a temperature of 13 - 30 °C. Avoid smoke, rodent damage, pest damage, waterlogging, etc. during storage.

[0101] Breaking seed dormancy: In July, select plump and uniformly sized wild Sabina vulgaris seeds, soak them in a HgCl2 solution with a mass concentration of 0.12% for 8 min and then take them out, and rinse them repeatedly 4 times with distilled water. Soak the disinfected seeds in a gibberellin solution with a concentration of 300 mg / L for 68 h, stir once every 6 h. After soaking, blot the water on the surface of the seeds, rinse them repeatedly 5 times with distilled water, and then place them in a light incubator for germination. The daily light time is 14 h / day, the light intensity is 3000 Lx, the temperature during light culture is 32 °C, the temperature during dark culture is 22 °C, and the germination time is 10 d.

[0102] Sowing: Sowing is carried out in early July in a greenhouse. Non-woven fabric seedling pots with a diameter of 8cm and a height of 10cm are used as sowing containers. Sand with a salt content of less than 500mM is selected as the sowing substrate. Before sowing, the sand is dried at 100℃ for 48 hours. A 6.5cm layer of fine sand with a particle size of 180-200μm is laid in the seedling pot. Then, the pre-germinated seeds are sown on the sand surface, 10 seeds per seedling pot, and covered with 1.8cm of coarse sand with a particle size of 300-350μm. After sowing, water is applied at a rate of 1kg / pot. After the water has completely soaked in and there is no standing water on the sand surface, another 0.5cm layer of fine sand is covered on the sand surface to prevent moisture evaporation.

[0103] Seedling management: Before emergence, water twice daily to keep the sand surface moist. Seedlings will emerge 5 days after sowing. After emergence, reduce watering frequency appropriately, but keep the sand surface moist. 15 days after sowing, seedlings will reach a height of 8-10cm. During the seedling stage, maintain an indoor temperature of 34℃. If the greenhouse temperature exceeds 35℃, open the shade netting promptly and increase the frequency of water spraying in the morning and evening to lower the sand surface temperature.

[0104] Seedling drought resistance training: When the seedlings reach a height of about 10cm and fine roots are emerging from the nutrient pots, they can be moved to a pre-prepared nursery outside the greenhouse. The nursery should be covered with sand about 10cm thick. Then, the nutrient pots should be neatly placed in the nursery, filling the gaps between the pots with fine sand until they are level with the pots. The gaps between the pots and the nursery ridges should also be filled with sand until they are level with the pots. Then, water thoroughly with a large amount of water. After that, water thoroughly every 5 days, then every 7 days after two waterings, then every 10 days after two more waterings. After one watering, the seedlings will be 15-20cm tall, and most of the roots will have emerged from the nutrient pots, reaching a length of 8-10cm. At this point, they are ready for transplanting.

[0105] Set up row-type sand barriers: Select gently shifting sand dunes with a vegetation cover of 1.8% for seedling transplantation. Set up plastic mesh sand barriers with a row spacing of 1.5m at a distance of 20m in front of the windward slope, perpendicular to the wind direction, so that they cover the entire sand dune. The sand barriers should be 30cm above the sand surface and the remaining 30cm buried in the sand. The width of the sand barriers should be 60cm and the length should be 426m.

[0106] Target dune pre-treatment: First, water the dunes with sand barriers set up to make the dry sand layer connect with the wet sand layer. Then, dig ditches and form ridges. The ditches are made into isosceles trapezoids with the angle between the bottom and the waist of the isosceles trapezoid being 120°, the bottom width of the ditch being 20 cm, the depth of the ditch being 30 cm, and the width of the ridge being 20 cm. Finally, cover the ditches and ridges with a 2.0 m wide degradable white plastic film, press it firmly, evacuate the gas under the film, cover 5 cm of fine sand at the bottom of the ditch, dig ditches at the edges of the ridges, put the extra 20 cm of film on both sides into the ditches, and then cover with sand and press firmly to make the sand connect with the plastic mesh sand barrier. Finally, lay a layer of clay with a thickness of 3 - 5 cm on the 15 cm of sand in contact with the sand barrier on both sides, and water the clay. Two ridges and one ditch form a row with a row spacing of 55 cm. Each row is carried out according to the steps of watering + digging ditches + forming ridges + covering with film + pressing the edges + sealing the edges + making holes + transplanting + sealing the holes + watering.

[0107] Transplanting is carried out at the beginning of August. Two days before lifting the seedlings, flood irrigation is used to thoroughly water the seedlings. On the third day, lift the seedlings, and avoid damaging the fine roots outside the nutrient pots when lifting the seedlings. Spray an appropriate amount of water before transportation to avoid excessive water loss and wilting of the seedlings. Holes are drilled in the bottom of the ditch in advance at a distance of 1.5 m, with the hole diameter being 10 cm and the depth being 12 cm. Transplant the seedlings into the dug holes, and then cover with sand to completely bury the nutrient pots in the sand, exposing the seedlings, and seal the surrounding of the holes tightly with clay. Water after transplantation, 1.2 kg per pot. The seedlings in adjacent two rows show a "pin" shape, that is, the seedlings in the second row are planted in the middle position between two seedlings in the corresponding first row, with a plant spacing of 1.5 m and a row spacing of 1.5 m.

[0108] Comparative Example 1

[0109] The traditional method of seed collection is to go to the desert for artificial collection many times during the seed maturity period. The specific method is to put a plastic bag on the mature ear, and then shake the ear, and the seeds fall into the plastic bag. The average cost per person per day is charged at 300 yuan (including collection fee and transportation fee), and about 100 g of seeds are collected per day. Therefore, the collection cost is 3000 yuan / kg.

[0110] Comparative Example 2

[0111] [[ID=!15]]Different from Example 1, the sand whip seeds are not subjected to any germination treatment.

[0112] Experimental Example 1. Comparison of seed collection costs

[0113] Comparison of seed collection costs between the examples and Comparative Example 1: The seeds collected in each bag are 2.2 - 6.2 g (the size is related to the growth status of the sand whip). If the service life of the seed bags and steel bars is 3 years, calculated at 50 bags per hour per person and 15 yuan per hour, the seed cost is 240 - 600 yuan / kg; calculated at 5 years, it is .....

[0114] Experimental Example 2. Statistics on Seed Germination Rate and Emergence Rate

[0115] Seed germination rate was calculated 11 days after germination. When the seedling height reached the requirements for transplanting, the emergence rate was calculated. The results are shown in Table 1.

[0116] Table 1 Germination rate and emergence rate of wild *Vallisneria natans* seeds

[0117] Germination rate (%) Emergence rate (%) Example 1 95.3 86.5 Example 2 97.6 87.1 Example 3 96.2 88.0 Comparative Example 2 58.3 38.6

[0118] As shown in Table 1, the method for raising wild sand pepper seeds of the present invention improves the germination rate and emergence rate, with a germination rate of over 95% and an emergence rate of over 85%.

[0119] Experimental Example 3. Statistics on the Survival Rate of Wild Sand Whip in the Same Year

[0120] At the end of October, the height of the seedlings was counted, using the maximum height of each seedling protruding from the sand surface in each nutrient pot as the standard, measured with a steel tape measure. Simultaneously, the survival rate was calculated row by row for each nutrient pot; the statistical standard was that any seedling protruding from the sand surface was considered a survival. The results are shown in Table 2.

[0121] Table 2 Height and survival rate of wild *Sagittaria sagittifolia*

[0122] Seedling height (cm) Survival rate (%) Example 1 30.6 72.6 Example 2 34.7 73.5 Example 3 32.5 71.9

[0123] As shown in Table 2, in October of the year of transplanting, the average height of wild sand whips was between 30 and 35 cm, and the survival rate could reach over 70%.

[0124] As can be seen from the above embodiments, the present invention provides a method for cultivating wild sand pepper seedlings. When wild sand pepper seeds are cultivated and planted according to the method of the present invention, the germination rate is above 95%, the emergence rate is above 85%, and in the year of transplanting, the average height of wild sand pepper is between 30 and 35 cm, with a survival rate of over 70%.

[0125] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for cultivating seedlings of *Vernicia fordii*, characterized in that, Includes the following steps: Step 1: Seed Collection (1.1) From early June to late October, cover the plump ears of seeds with nylon net seed bags and seal the opening of the seed bags to prevent the seeds from falling onto the sandy ground; (1.2) Insert the steel bar next to the tiller and tie it together with the tiller and the seed bag to prevent the tiller from being blown over by the wind after the bag is put on; (1.3) From late July to early November, cut the seed bags along with the fruit spikes and collect the seeds. During this process, do not damage the seed bags and steel bars. Recycle them together to reduce the cost of seed collection. Step 2: Breaking Seed Dormancy (2.1) Remove the seeds from the seed coat and store them in a well-ventilated environment at room temperature when the seed moisture content is less than 14%. (2.2) After screening out plump and uniformly sized seeds, disinfect them and rinse them with distilled water 3 to 5 times; (2.3) After disinfection, the seeds are soaked in gibberellin at a concentration of 180~300mg / L for 48~72h, and then germinated in a light incubator; Step 3: Sowing (3.1) The sowing substrate is sand, with fine sand particles of 180~200μm and coarse sand particles of 300~350μm. The bottom 5.5~6.5cm of fine sand and the top 1.8~2.5cm of coarse sand. Before filling the sand, dry the sand at 100℃ for 48h to kill insect eggs and other seeds. (3.2) From late April to early July, in a greenhouse, when the sand surface temperature reaches above 20℃, sow seeds. First, fill non-woven fabric seedling pots with fine sand, then place the seedling pots on the seedbed, and sow the germinated seeds in the center of the sand surface of the seedling pots. Finally, cover the seeds with 1.8~2.5cm of coarse sand. Water after sowing. After the water has fully penetrated, when there is no water accumulation on the sand surface, cover the sand surface with another 0.25~0.5cm of fine sand to prevent water evaporation. The seedling pots are 8cm wide, with an open end that can be opened to fill with sand, and 10cm long. Each seedling pot contains 8~10 seeds. Step 4: Seedling cultivation (4.1) Water management: Before the seedlings emerge, water them in the morning and evening to keep the sand surface moist. After the seedlings emerge, reduce the number of waterings, but keep the sand surface from drying out. (4.2) Temperature management: When the greenhouse temperature is greater than 35℃, the shade net should be opened in time, and the number of water sprays should be increased in the morning and evening to reduce the temperature of the sand surface; Step 5: Seedling drought resistance training When the seedlings reach a height of 8-10cm, they are moved to a pre-prepared nursery outside the greenhouse. The nursery is covered with sand to a thickness of 10-20cm. Then, the seedling pots are neatly moved into the nursery. The gaps between the seedling pots are filled with fine sand to the same height as the seedling pots. The gaps between the seedling pots and the nursery ridges are also filled with sand to the same height as the seedling pots. Then, the seedlings are flooded with water. Step Six: Setting up row-type sand barriers (6.1) Selection of mobile dunes: Select gently moving dunes with vegetation cover of less than 2%; (6.2) Setting up row-type sand barriers: 10-20m in front of the windward slope, set up plastic mesh sand barriers with a row spacing of 1.5m along a line perpendicular to the wind direction, so that they cover the entire sand dune. The sand barriers are 30cm above the sand surface and the remaining 30cm are buried in the sand. The width of the sand barriers is 60cm and the length depends on the size of the sand dune. Step 7: Pre-treatment of the target sand dunes Water the target sand dune, then dig an isosceles trapezoidal trench, wider at the top and narrower at the bottom. Make ridges on both sides of the trench, and finally cover the trench and ridges with biodegradable white plastic film, compact it to expel the gas under the film, cover the bottom of the trench with 2-3 cm of fine sand, dig trenches on the sides of the ridges, put the excess plastic film on both sides into the trenches, then cover with sand and compact it. Finally, spread a layer of wet clay 2-3 cm thick and 8-10 cm wide on the compacted sand surface, and make planting holes at the bottom of the isosceles trapezoidal trench. Step 8: Transplanting Transplanting should be done from late June to early August. Water thoroughly with plenty of water 1-2 days before lifting the seedlings. Lift the seedlings on the 3rd-4th day. After the seedlings are transported to their destination, transplant them into holes at the bottom of the trench. Then cover them with sand so that the seedling pots are completely buried in the sand, leaving the seedlings exposed. Seal the holes tightly with clay. Water 1-1.5 kg / pot after transplanting.

2. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, The seed bags in step one need to be specially made, with a width of 25~30cm × a length of 70~80cm. The mesh should be rectangular, with a length of 5.5~7.0mm and a width of 1.0~1.5mm. The mesh should be smaller in areas with less rainfall and larger in areas with more rainfall.

3. The method for cultivating *Vernicia fordii* seedlings according to claim 1, characterized in that, In step (2.2), the seeds are disinfected by soaking in HgCl2 solution; the mass concentration of the HgCl2 solution is 0.08~0.12%, and the soaking time is 8~12 min.

4. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, In step (2.3), the seed germination light culture time is 14h, the temperature is 28~35℃, the light intensity is 2000~3500Lx, and the dark culture time is 10h, the temperature is 20~25℃.

5. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, The salt content of the sowing substrate in step (3.1) is less than 500 mM.

6. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, In step one, the seed storage temperature is 13~30℃; in step 3.2, the temperature in the greenhouse after sowing is maintained at 20~35℃.

7. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, The flood irrigation described in step five is as follows: after the initial irrigation, irrigate once every 4 to 6 days, for a total of 2 irrigations; then irrigate once every 7 to 8 days, for a total of 2 irrigations; and then irrigate once every 9 to 10 days, for a total of 1 irrigation.

8. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, The watering standard for the target sand dunes in step seven is as follows: the water poured on the surface of the sand dunes should penetrate into the wet sand layer and connect with it; the bottom and waist angle of the isosceles trapezoidal trench is 120°, the bottom width is 20cm, the trench depth is 30cm, the ridge width is 20cm, two ridges make one trench and one row, the row spacing is 55cm, and each row of strip sand barriers only has one trench and two ridges. The specific method is: watering + trenching + ridge making + mulching + edge pressing + edge sealing + hole making + transplanting + sealing + watering.

9. The method for cultivating *Vaccaria segetalis* seedlings according to claim 1, characterized in that, When transplanting, the plant spacing should be 1.0~1.5m and the row spacing should be 1.5m. The row spacing mentioned is the row spacing of the row strip sand barrier.

10. The method for cultivating *Vigna arvense* seedlings according to claim 1, characterized in that, The seeds mentioned in step one were collected from Minqin, Gulang, Dengkou or Ordos.