Trehalose soil conditioner as well as preparation method and application thereof

Abstract

The invention relates to the technical field of soil improvement, in particular to a trehalose soil conditioner as well as a preparation method and application thereof. The trehalose soil conditioner disclosed by the invention is prepared from the following components in parts by mass: 40 to 60 parts of trehalose, 20 to 40 parts of humic acid, 10 to 20 parts of montmorillonite, 5 to 12 parts of glycine betaine, 20 to 50 parts of monosodium glutamate waste liquid, 1 to 5 parts of arginine, 2 to 10 parts of proline, 60 to 100 parts of kitchen garbage and 0.03 to 0.1 part of fermentation bacterium agent, the fermentation inoculant comprises the following inoculants: a bacillus cereus inoculant, a bacillus mucilaginosus inoculant and aspergillus oryzae in a mass ratio of (1-3): (2-6): (1-3). The trehalose soil conditioner prepared from the components can improve the drought resistance of crops by changing the activity of root microorganisms, so that the yield of the crops is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of soil improvement, and particularly relates to a trehalose soil conditioner and its preparation method and application. Background Art

[0002] Drought is the main limiting factor for global agricultural production. Especially in arid and semi-arid regions, water shortage seriously affects crop growth and yield. At present, the main strategies to improve crop drought resistance include traditional breeding, biotechnological improvement, and chemical regulation, etc., but there are problems such as long cycle, high cost, or environmental risks. Therefore, it is crucial to find green and efficient drought resistance regulation methods. Research shows that plants can adapt to the environment by regulating root exudates under drought stress, and among them, trehalose plays an important role in stabilizing the structure of biological macromolecules, regulating physiological metabolism, and rhizosphere microbial communities. However, its application research in promoting crop drought resistance is still relatively limited. A new and sustainable solution to improve crop drought resistance and growth performance is urgently needed.

[0003] Based on this, the present invention is proposed. Summary of the Invention

[0004] The purpose of the present invention is to provide a trehalose soil conditioner and its preparation method and application.

[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0006] The present invention provides a trehalose soil conditioner, which comprises the following components in parts by mass:

[0007] 40 - 60 parts of trehalose, 20 - 40 parts of humic acid, 10 - 20 parts of montmorillonite, 5 - 12 parts of betaine, 20 - 50 parts of monosodium glutamate waste liquid, 1 - 5 parts of arginine, 2 - 10 parts of proline, 60 - 100 parts of kitchen waste, 0.03 - 0.1 part of fermentation bacterium agent;

[0008] The fermentation bacterium agent comprises the following bacterium agents in mass ratio:

[0009] Bacillus cereus bacterium agent: Bacillus mucilaginosus bacterium agent: Aspergillus oryzae is 1 - 3: 2 - 6: 1 - 3.

[0010] Preferably, the monosodium glutamate waste liquid is the waste liquid generated during the production of monosodium glutamate.

[0011] Preferably, the initial viable count of the Bacillus cereus bacterium agent ≥ 1.0×10 11 cfu / g;

[0012] The initial viable count of the Bacillus mucilaginosus bacterium agent ≥ 1.0×10 10 cfu / g;

[0013] The initial viable count of Aspergillus oryzae is ≥ 1.0×10 10 cfu / g.

[0014] The present invention also provides a method for preparing a trehalose soil conditioner, comprising the following steps:

[0015] (1) Weigh each component according to the stated parts by mass;

[0016] (2) Mix kitchen waste with a fermentation inoculant to obtain a mixture, adjust the water content of the mixture to 60 - 65%, and ferment for 8 - 10 days to obtain a fermented material;

[0017] (3) Mix trehalose, humic acid, montmorillonite, betaine and monosodium glutamate waste liquid, ultrasonicate for 1 - 2 h, dry and pulverize to obtain an active component;

[0018] (4) Mix the fermented material, active component, arginine and proline, and granulate to obtain a trehalose soil conditioner.

[0019] Preferably, the temperature of the fermentation in step (2) is 40 - 60 °C.

[0020] Preferably, the power of the ultrasonic treatment in step (3) is 450 - 550 W.

[0021] Preferably, the temperature of the drying in step (3) is 50 - 60 °C, and the drying time is 8 - 16 h;

[0022] The particle size of the pulverization in step (3) is 10 - 20 nm.

[0023] The present invention also provides the application of the above-mentioned trehalose soil conditioner in improving the activity of beneficial microorganisms in soil.

[0024] The present invention also provides the application of the above-mentioned trehalose soil conditioner in improving the drought resistance and growth performance of crops.

[0025] The present invention also provides the application of the trehalose soil conditioner prepared by the above-mentioned preparation method in improving the activity of beneficial microorganisms in soil, improving the drought resistance and growth performance of crops.

[0026] The present invention provides a trehalose soil conditioner and its preparation method and application. The trehalose soil conditioner of the present invention comprises trehalose, humic acid, montmorillonite, betaine, monosodium glutamate waste liquid, arginine, proline, kitchen waste, and a fermentation bacterium agent; the fermentation bacterium agent comprises a bacillus cereus bacterium agent, a bacillus mucilaginosus bacterium agent, and an aspergillus oryzae. The fermentation bacterium agent of the present invention can effectively decompose proteins, fats, cellulose, and starch in kitchen waste, and improve the availability of the fermentation material. In the present invention, trehalose, humic acid, betaine, and montmorillonite are placed in the monosodium glutamate waste liquid for ultrasonic treatment, further improving the adsorption performance of trehalose, humic acid, betaine, and montmorillonite, better improving the physical and chemical properties of the soil, enhancing soil fertility, improving the stress resistance of crops, and promoting crop growth. The monosodium glutamate waste liquid contains a large amount of organic matter and glutamic acid, which not only provides organic matter for crop growth, but also synergizes with arginine and proline to enhance the stress resistance of crops and improve crop growth performance.

[0027] Kitchen waste and monosodium glutamate waste liquid are waste materials in the production process. The present invention uses them in soil improvement and plays an excellent role, turning waste into treasure and providing new ideas for soil improvement and crop growth.

[0028] The components of the present invention synergistically have the effects of promoting the activity of beneficial soil microorganisms, improving the stress resistance and growth performance of crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 are the results of microbial diversity indices in soils with different treatments;

[0030] Figure 2 are the community compositions of microorganisms in soils with different treatments;

[0031] Figure 3 are the effects of soils with different treatments on wheat growth (the left figure shows the growth of wheat under normal conditions, and the right figure shows the growth of wheat under drought conditions);

[0032] Figure 4 are the effects of different treatments under drought conditions on the plant height of wheat;

[0033] Figure 5 are the effects of different treatments under drought conditions on the dry weight of the above-ground part of wheat. DETAILED DESCRIPTION OF THE INVENTION

[0034] The present invention provides a trehalose soil conditioner, which comprises the following components in parts by mass:

[0035] Trehalose 40 - 60 parts, preferably 45 - 55 parts, more preferably 50 parts;

[0036] Humic acid 20 - 40 parts, preferably 25 - 35 parts, more preferably 30 parts;

[0037] 10 - 20 parts of montmorillonite, preferably 12 - 18 parts, more preferably 15 parts;

[0038] 5 - 12 parts of betaine, preferably 7 - 10 parts, more preferably 8 parts;

[0039] 20 - 50 parts of monosodium glutamate waste liquid, preferably 30 - 40 parts, more preferably 35 parts;

[0040] 1 - 5 parts of arginine, preferably 2 - 4 parts, more preferably 3 parts;

[0041] 2 - 10 parts of proline, preferably 4 - 8 parts, more preferably 6 parts;

[0042] 60 - 100 parts of kitchen waste, preferably 70 - 90 parts, more preferably 80 parts;

[0043] 0.03 - 0.1 part of fermentation inoculant, preferably 0.05 - 0.08 part, more preferably 0.06 part;

[0044] The fermentation inoculant includes inoculants in the following mass ratio:

[0045] Bacillus cereus inoculant: Bacillus mucilaginosus inoculant: Aspergillus oryzae = 1 - 3:2 - 6:1 - 3, preferably 2:4:2.

[0046] In the present invention, the monosodium glutamate waste liquid is the waste liquid generated during the production of monosodium glutamate, and the monosodium glutamate waste liquid is sourced from Meihua Monosodium Glutamate Co., Ltd.

[0047] In the present invention, the initial viable count of the Bacillus cereus inoculant ≥ 1.0×10 11 cfu / g, preferably 1.0×10 11 cfu / g, purchased from Henan Jiubang Biotechnology Co., Ltd.;

[0048] The initial viable count of the Bacillus mucilaginosus inoculant ≥ 1.0×10 10 cfu / g, preferably 1.0×10 10 cfu / g, purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd.;

[0049] The initial viable count of the Aspergillus oryzae ≥ 1.0×10 10 cfu / g, preferably 1.0×10 10 cfu / g, purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd.

[0050] In the present invention, the kitchen waste is the kitchen waste separated from the domestic waste classification station in Yuquan District, Hohhot. After removing the non-degradable components such as porcelain chips, glass, plastics, and metals, the kitchen waste is crushed into a substance with a particle size of 2 cm.

[0051] The present invention also provides a preparation method of a trehalose soil conditioner, which includes the following steps:

[0052] (1) Weigh each component according to the stated mass parts;

[0053] (2) Mix the kitchen waste with a fermentation inoculant to obtain a mixture, adjust the water content of the mixture to 60-65%, and ferment for 8-10 days to obtain a fermented material;

[0054] (3) Mix trehalose, humic acid, montmorillonite, betaine, and monosodium glutamate waste liquid, ultrasonicate for 1-2 h, dry, and crush to obtain an active component;

[0055] (4) Mix the fermented material, active component, arginine, and proline, and granulate to obtain a trehalose soil conditioner.

[0056] In the present invention, the temperature of the fermentation in step (2) is 40-60 °C, preferably 50 °C; the fermentation time is preferably 9 days.

[0057] In the present invention, the power of the ultrasonic wave in step (3) is 450-550 W, preferably 500 W; the ultrasonic time is preferably 1.5 h.

[0058] In the present invention, the temperature of the drying in step (3) is 50-60 °C, preferably 55 °C, and the drying time is 8-16 h, preferably 12 h;

[0059] The particle size of the crushing in step (3) is 10-20 nm, preferably 15 nm.

[0060] The present invention also provides the application of the trehalose soil conditioner in improving the activity of beneficial microorganisms in the soil.

[0061] The present invention also provides the application of the trehalose soil conditioner in improving the drought resistance and growth performance of crops.

[0062] The present invention also provides the application of the trehalose soil conditioner prepared by the preparation method in improving the activity of beneficial microorganisms in the soil, improving the drought resistance and growth performance of crops.

[0063] The following combines examples to detail the solutions provided by the present invention, but they cannot be understood as limiting the protection scope of the present invention.

[0064] In the embodiments and comparative examples of the present invention, the kitchen waste is the kitchen waste separated from the domestic waste classification station in Yuquan District, Hohhot. After removing non-degradable components such as porcelain chips, glass, plastics, and metals, the kitchen waste is crushed into a substance with a particle size of 2 cm.

[0065] In the embodiments and comparative examples of the present invention, the monosodium glutamate waste liquid is the waste liquid generated during the production of monosodium glutamate, and the monosodium glutamate waste liquid is sourced from Meihua Monosodium Glutamate Co., Ltd.

[0066] In the embodiments and comparative examples of the present invention, the Bacillus cereus bacterium agent is purchased from Henan Jiubang Biotechnology Co., Ltd., and the viable bacteria count is 1.0×10 11 cfu / g; the Bacillus mucilaginosus bacterium agent is purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd., and the viable bacteria count is 1.0×10 10 cfu / g; the Aspergillus oryzae is purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd., and the viable bacteria count is 1.0×10 10 cfu / g.

[0067] Example 1

[0068] Take 0.1 kg of Bacillus cereus bacterium agent, 0.5 kg of Bacillus mucilaginosus bacterium agent and 0.3 kg of Aspergillus oryzae, and mix them evenly to obtain a fermentation bacterium agent.

[0069] Mix 80 kg of kitchen waste with 0.06 kg of the fermentation bacterium agent to obtain a mixture, adjust the water content of the mixture to 65%, and ferment at 50°C for 8 days to obtain a fermented material.

[0070] Mix 50 kg of trehalose, 40 kg of humic acid, 10 kg of montmorillonite, 5 kg of betaine and 40 kg of monosodium glutamate waste liquid, and ultrasonicate at 500 W for 1.5 h to obtain an ultrasonic mixture. Place the ultrasonic mixture in a constant temperature drying chamber at 55°C and dry for 12 h to obtain a dried product. Crush the dried product to a particle size of 15 nm to obtain an active component.

[0071] Mix the fermented material, the active component with 3 kg of arginine and 8 kg of proline, dry them, and then granulate them in a granulator to obtain a trehalose soil conditioner.

[0072] Example 2

[0073] Take 0.3 kg of Bacillus cereus bacterium agent, 0.2 kg of Bacillus mucilaginosus bacterium agent and 0.2 kg of Aspergillus oryzae, and mix them evenly to obtain a fermentation bacterium agent.

[0074] Mix 100 kg of kitchen waste with 0.1 kg of the fermentation bacterium agent to obtain a mixture, adjust the water content of the mixture to 60%, and ferment at 55°C for 10 days to obtain a fermented material.

[0075] Mix 40 kg of trehalose, 30 kg of humic acid, 15 kg of montmorillonite, 12 kg of betaine and 20 kg of monosodium glutamate waste liquid, and ultrasonicate for 2 h at 450 W to obtain an ultrasonic mixture. Place the ultrasonic mixture in a constant temperature drying chamber at 50 °C and dry for 16 h to obtain a dried product. Crush the dried product to a particle size of 10 nm to obtain an active component.

[0076] Mix the fermented material, the active component with 1 kg of arginine and 10 kg of proline, dry, and then granulate in a granulator to obtain a trehalose soil conditioner.

[0077] Example 3

[0078] Take 0.2 kg of Bacillus cereus bacterium agent, 0.6 kg of Bacillus mucilaginosus bacterium agent and 0.1 kg of Aspergillus oryzae, mix them evenly to obtain a fermented bacterium agent.

[0079] Mix 60 kg of kitchen waste with 0.03 kg of the fermented bacterium agent to obtain a mixture, adjust the water content of the mixture to 65%, and ferment at 60 °C for 9 d to obtain a fermented material.

[0080] Mix 60 kg of trehalose, 20 kg of humic acid, 20 kg of montmorillonite, 8 kg of betaine and 50 kg of monosodium glutamate waste liquid, and ultrasonicate for 1 h at 550 W to obtain an ultrasonic mixture. Place the ultrasonic mixture in a constant temperature drying chamber at 60 °C and dry for 10 h to obtain a dried product. Crush the dried product to a particle size of 20 nm to obtain an active component.

[0081] Mix the fermented material, the active component with 5 kg of arginine and 2 kg of proline, dry, and then granulate in a granulator to obtain a trehalose soil conditioner.

[0082] Comparative Example 1

[0083] Design Comparative Example 1 according to the scheme of Example 1. Different from Example 1, in the comparative example, the same mass of uridine is used to replace the trehalose in Example 1.

[0084] Experimental Example 1

[0085] Take wheat root soil, mix it evenly, and then separately put it into different flowerpots. The flowerpots are of the same size. Each flowerpot is filled with 3 / 4 of the flowerpot volume of soil, that is, each flowerpot is filled with 2 kg of soil. Randomly divide the flowerpots into 2 groups, the trehalose soil conditioner group (Trehalose), the uridine soil conditioner group (Uridine). When the water content of the soil in each flowerpot reaches 40 ± 2%, enter the drought stress stage, maintain the water content of the soil in each flowerpot at 40 ± 2%, and keep it for 2 months. Then, perform amplicon sequencing on the soils of the two groups to analyze the changes in the microbial diversity index in the soils of different treatments. The results are as Figure 1As shown. The composition and relative abundance of the soil microbial community in each group of flower pots were analyzed, and the results are as Figure 2 shown.

[0086] Figures 1 - 2 shown that the alpha diversity of bacteria in the soil treated with trehalose soil conditioner was significantly higher than that in the uridine soil conditioner treatment group (P<0.01, Figure 1 ). Further analysis of the composition and relative abundance of the soil microbial community found that compared with the treatment with uridine soil conditioner, the relative abundances of Pseudomonas, Bacillus, Agromyces, Rhodococcus, Rhodoplanes, Microbacterium, and Streptomyces in the soil treated with trehalose soil conditioner were all significantly increased ( Figure 2 ). These results indicate that the trehalose soil conditioner promotes the drought resistance of crops by regulating the composition of the rhizosphere microbial community.

[0087] Experimental Example 2

[0088] (1) Under normal conditions, the effects of different treated soils on wheat growth:

[0089] Take the soil suitable for wheat growth, mix it evenly, and put it into flower pots respectively. Each flower pot is filled with 2 kg of soil. The flower pots are randomly divided into 3 groups: the trehalose soil conditioner group (Trehalose), the uridine soil conditioner group (Uridine), and the sterile water group (Sterile Water). 10 g of the trehalose soil conditioner of Example 1 was applied to each flower pot in the trehalose soil conditioner group; 10 g of the uridine soil conditioner of Comparative Example 1 was applied to each flower pot in the uridine soil conditioner group. After application, water thoroughly. No soil conditioner was applied to the sterile water, and the soil needed to be watered thoroughly with sterile water. Spring wheat materials were sown in each flower pot, and the water content of the soil in each flower pot was maintained at 65%. The growth of spring wheat at 15 days after sowing was as Figure 3 shown.

[0090] (2) Under drought conditions, the effects of different treated soils on wheat growth:

[0091] Take the soil suitable for wheat growth, mix it evenly, and place it separately in flower pots. Each flower pot contains 2 kg of soil. Randomly divide the flower pots into 3 groups: the trehalose soil conditioner group (Trehalose), the uridine soil conditioner group (Uridine), and the sterile water group (Sterile Water). Apply 10 g of the trehalose soil conditioner from Example 1 to each flower pot in the trehalose soil conditioner group; apply 10 g of the uridine soil conditioner from Comparative Example 1 to each flower pot in the uridine soil conditioner group. After application, water thoroughly. Do not apply any soil conditioner to the sterile water, and use sterile water to thoroughly water the soil. When the water content of the soil in each group of flower pots drops to 42 ± 2% (entering the drought stress state), sow spring wheat, and keep the water content of the soil in each flower pot at 42 ± 2%. Sow spring wheat materials in each flower pot. When sowing for 20 days, the growth of spring wheat in each group is as Figure 3 shown.

[0092] Figure 3 shown that whether under normal conditions or under drought treatment, the growth trend of spring wheat planted in the soil treated with the trehalose soil conditioner is better than that of the uridine soil conditioner treatment group and the sterile water treatment group.

[0093] Further prove that the trehalose soil conditioner prepared by the present invention can promote the drought resistance and growth of wheat.

[0094] Experimental Example 3

[0095] Statistically analyze the plant height of the wheat sown after treating the soil with different treatments under drought conditions in Experimental Example 2. The results are as Figure 4 shown.

[0096] Statistically analyze the dry weight of the above-ground part of the wheat sown after treating the soil with different treatments under drought conditions in Experimental Example 2. The results are as Figure 5 shown.

[0097] Figures 4 - 5 shown that under drought conditions, after treating the soil with the trehalose soil conditioner, the growth of the plant height of the sown wheat is significantly improved compared with the sterile water treatment group. The plant height of the trehalose soil conditioner treatment group is 15.15% higher than that of the sterile water treatment group; it is 10.22% higher than that of the uridine soil conditioner treatment group. At the same time, the dry weight of the above-ground part of the wheat treated with the trehalose soil conditioner increases by 48.11% compared with the sterile water treatment group and increases by 17.16% compared with the uridine soil conditioner treatment group. This shows that under drought stress conditions, the soil treated with the trehalose soil conditioner of the present invention helps to increase the plant height and dry weight of wheat and promote the growth and development of plants.

[0098] Application Example 1

[0099] In 2023, in the wheat planting area of Hohhot, the soil was treated with the trehalose soil conditioner of Example 1 to plant Bamai 10.

[0100] The trehalose soil conditioner of Example 1 was applied at a rate of 50 kg / mu 5 days before sowing wheat. Control 1 was applied with the same mass of the uridine soil conditioner of Comparative Example 1, and Control 2 was applied with the same mass of trehalose. After application, water was poured, and the rest was planted by conventional methods.

[0101] After harvesting the wheat, it was weighed to compare the effects of soils treated by different methods on wheat yield. The results are shown in Table 1.

[0102] Table 1 Effects of soils treated by different methods on wheat yield

[0103] Group Example 1 Group Control 1 Control 2 Yield / kg / mu 428 389 362

[0104] Table 1 shows that after treating the soil with the trehalose soil conditioner of Example 1, the wheat yield was significantly increased.

[0105] From the above examples, it can be seen that the present invention provides a trehalose soil conditioner and its preparation method and application. The trehalose soil conditioner of the present invention includes trehalose, humic acid, montmorillonite, betaine, monosodium glutamate waste liquid, arginine, proline, kitchen waste, and fermentation bacterium agent; the fermentation bacterium agent includes bacillus cereus bacterium agent, bacillus mucilaginosus bacterium agent, and aspergillus oryzae. The components of the present invention synergistically have the effects of promoting the activity of beneficial soil microorganisms, improving the stress resistance and growth performance of crops.

[0106] The above is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A trehalose soil conditioner, characterized in that: The composition includes the following components in parts by weight: 40-60 parts of trehalose, 20-40 parts of humic acid, 10-20 parts of montmorillonite, 5-12 parts of betaine, 20-50 parts of monosodium glutamate waste liquid, 1-5 parts of arginine, 2-10 parts of proline, 60-100 parts of kitchen waste, and 0.03-0.1 parts of fermentation agent; The fermentation agent includes the following mass ratio of agents: The ratio of Bacillus cereus agent: Bacillus jelly agent: Aspergillus oryzae is 1-3:2-6:1-3.

2. The trehalose soil conditioner according to claim 1, characterized in that The monosodium glutamate waste liquid is the waste liquid generated in the process of manufacturing monosodium glutamate.

3. The trehalose soil conditioner according to claim 2, characterized in that The initial viable count of the Bacillus cereus inoculant is ≥ 1.0×10 11 cfu / g; The initial viable count of the jelly-like Bacillus inoculum is ≥ 1.0×10 10 cfu / g; The initial viable count of Aspergillus oryzae is ≥ 1.0×10 10 cfu / g.

4. A method for preparing a trehalose soil conditioner, characterized in that: The steps include: (1) Weighing each component according to the mass fractions described in any one of claims 1 to 3; (2) mixing kitchen waste with a fermentation agent to obtain a mixture, adjusting the water content of the mixture to 60 to 65%, and fermenting for 8 to 10 days to obtain a fermented material; (3) mixing trehalose, humic acid, montmorillonite, betaine and monosodium glutamate waste liquid, ultrasonicating for 1 to 2 hours, drying, and crushing to obtain an active component; (4) The fermentation material, active components, arginine and proline are mixed and granulated to obtain a trehalose soil conditioner.

5. The preparation method according to claim 4, characterized in that: The fermentation temperature in step (2) is 40-60°C.

6. The preparation method according to claim 5, characterized in that: The power of the ultrasound in step (3) is 450-550W.

7. The preparation method according to claim 6, characterized in that: The drying temperature in step (3) is 50-60° C. and the drying time is 8-16 hours; The particle size of the crushed product in step (3) is 10 to 20 nm.

8. Use of the trehalose soil conditioner according to any one of claims 1 to 3 in improving the activity of beneficial microorganisms in soil.

9. Use of the trehalose soil conditioner according to any one of claims 1 to 3 in improving the drought resistance and growth performance of crops.

10. Use of the trehalose soil conditioner prepared by the preparation method according to any one of claims 4 to 7 in improving the activity of beneficial microorganisms in the soil and improving the drought resistance and growth performance of crops.

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