Strontium element concentration gradient-based coreius guichenoti fry otolith marking method and application
The round-mouth copper fish fry is labeled with strontium otile element elements through different concentration gradients, forming a differentiated strontium element fingerprint, which solves the problem that the existing technology cannot distinguish different release batches, and realizes accurate distinction and tracking of fry, reducing the labeling cost and management difficulty.
Patent Information
- Application Number
- CN202510339055.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-21
- Publication Date
- 2025-06-24
AI Technical Summary
The prior art cannot effectively distinguish the round-mouthed copper fish from different release batches, resulting in difficulty in tracking, evaluating and managing fry after proliferation.
Round-mouthed copper fish fry was labeled with strontium otolithic strontium element by different concentration gradients to form different strontium element fingerprints, and the batches belonging to the fry were analyzed and identified using electronic probes.
It realizes accurate distinction and tracking of fish fry of different release batches, reduces the labeling cost and management difficulty, and provides a reliable basis for the evaluation of effect after reproduction and release.
Smart Images
Figure CN120195208A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of proliferation and release marking methods, and specifically relates to a method for marking otolith strontium elements of round-mouthed copper fish fry and its application in proliferation and release. By marking with strontium elements of different concentration gradients, fry of different release batches can be effectively distinguished during the release process, providing a reliable technical means for tracking and management after proliferation and release. Background Art
[0002] Coreius guichenoti is an economic fish endemic to the upper reaches of the Yangtze River. Its stocking and reproduction activities are crucial to resource recovery, but the lack of efficient batch tracking technology limits the assessment of the survival rate of released fry and the optimization of stocking and reproduction strategies. Traditional labeling methods (such as fluorescent labeling and genetic labeling) cannot generate unique batch identifiers, resulting in inefficient resource management after release. Specifically: fluorescent labeling signals tend to decay with the growth of the fish or environmental changes, making it difficult to identify them stably in the long term, and it is difficult to distinguish between different release batches; genetic labeling relies on gene sequencing and complex pedigree analysis, requiring the collection of a large number of parental samples and the establishment of a genetic database, which is costly and has a high threshold for specialization. Neither of them can achieve multi-batch differentiation through simple regulation, resulting in a lack of reliable data support for the evaluation of population replenishment effects and resource management after stocking and reproduction.
[0003] The core goal of fishery resource enhancement and release is to restore the population and ecological balance, but the limitations of traditional technology directly restrict its effectiveness. Although existing methods can achieve individual marking, they fail to solve the key demand of multi-batch parallel management. For example, the enhancement and release of round-mouthed copper fish is mostly concentrated on the large-scale release of small-sized fry, which requires the marking technology to have both scalability and batch differentiation capabilities. However, traditional marking methods are difficult to meet practical application needs due to their low efficiency or high cost.
[0004] Otolith labeling technology forms characteristic element fingerprints in the otoliths of fish fry through the biomineralization of elements in the water, providing new ideas for labeling technology. Although strontium element labeling has been applied to some fish, its applicability in round-mouthed copper fish has not been verified, and there are two major technical gaps in existing research: the lack of concentration gradient design - the feasibility of generating differentiated element fingerprints by adjusting the strontium concentration gradient and then achieving batch differentiation has not been explored; standardized parameter gaps - there is a lack of systematic research on key parameters such as the strontium concentration range and immersion time required for labeling.
[0005] In addition, otolith marking technology faces common challenges in practical applications: there are interspecies differences in marking effects, and the scheme needs to be optimized for specific species (such as round-mouthed copper fish); existing methods only focus on individual marking, and have not built a batch identification system based on concentration gradients, and cannot support precise tracking of multiple batches.
[0006] In summary, developing a labeling method based on strontium concentration gradient, easy to operate and able to generate unique batch identification has become a key requirement to break through the bottleneck of roundmouth copper fish reproduction and release management. Summary of the invention
[0007] In view of the problems existing in the prior art, the object of the present invention is to provide a method for distinguishing batches of round-mouthed copper fish proliferation and release based on strontium element concentration gradient and its application. The current proliferation and release method cannot effectively distinguish different release batches, resulting in difficulties in tracking, evaluating and managing the fry. The present invention uses strontium chloride hexahydrate with different concentration gradients to mark the otolith strontium element of round-mouthed copper fish fry, and can distinguish different release batches by adjusting the strontium concentration. Since the element fingerprints formed in the otoliths of fry with strontium elements of different concentration gradients have obvious differences, different batches of released fry can be accurately distinguished. This feature provides an important means for tracking and managing the effects after proliferation and release.
[0008] In order to achieve the above object, the present invention adopts the following technical solution:
[0009] Each release batch independently corresponds to a strontium concentration mark (such as 20 mg / L, 40 mg / L, etc. in Examples 1-6). By setting discrete concentration gradients such as 20 mg / L, 40 mg / L, and 80 mg / L, each concentration corresponds to an independent release batch, and a differentiated strontium element fingerprint is formed in the otolith (such as edge area peaks, Sr content increases with concentration). Combined with electron probe analysis, the batch source can be uniquely identified. After the proliferation release, the otolith strontium element distribution map (such as Figure 8 The batch to which the fry belong can be uniquely identified based on the peak position and intensity difference, thus enabling parallel tracking and management of multiple batches.
[0010] The fry (about 60 days old) with a body length of about 4.5 cm is usually selected for proliferation and release. In order to meet the proliferation and release demand, the round-mouthed copper fish fry of 60-90 days old can be selected. In the present embodiment, the round-mouthed copper fish fry is the healthy round-mouthed copper fish fry after 60 days of seedling raising.
[0011] The otolith strontium element marking method of round-mouthed copper fish fry of the present invention can be applied to the proliferation and release of round-mouthed copper fish.
[0012] Advantages of the technical solution of the present invention:
[0013] The core of the present invention is to solve the three core problems of the traditional marking method, namely, high cost, complicated operation and inability to distinguish batches, by combining concentration gradient design with immersion technology, and provide an efficient, accurate and sustainable marking scheme for the proliferation and release of round-mouthed copper fish. Compared with the traditional marking method, there is no need for tail-by-tail operation or genetic analysis, which significantly reduces the marking cost and management difficulty of large-scale release. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 Schematic diagram of Sr content in otoliths of fry in the control group;
[0015] Figure 2 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 20 mg / L;
[0016] Figure 3 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 40 mg / L;
[0017] Figure 4 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 80 mg / L;
[0018] Figure 5 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 160 mg / L;
[0019] Figure 6 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 320 mg / L;
[0020] Figure 7 This is a schematic diagram of the Sr content in otoliths of labeled fry at a concentration of 640 mg / L;
[0021] Figure 8 Schematic diagram of the peak Sr content in otoliths of labeled fry at different concentrations. DETAILED DESCRIPTION
[0022] During the implementation process, different batches of round-mouthed copper fish fry were marked by adjusting the strontium concentration in the water (such as 20mg / L, 40mg / L, 80mg / L, 160mg / L, 320mg / L, 640mg / L). The strontium content in the otoliths of the fry at each concentration was different, forming a concentration fingerprint with obvious differences. These fingerprints can be used as identification of different release batches to facilitate subsequent tracking and management.
[0023] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings and embodiments.
[0024] It should be noted that the following examples are only used to demonstrate the embodiments of the present invention and do not limit the protection scope of the present invention. The concentration range of the present invention is not limited to the specific concentration values shown in the examples. As long as the expected marking effect can be achieved, any appropriate strontium concentration can be applied to the present invention.
[0025] The juvenile round-mouthed copper fish used in the following examples were artificially bred at the Xiangjiaba breeding and releasing station in Yibin City, Sichuan Province.
[0026] Examples 1-6 were all implemented according to the following steps: healthy round-mouthed copper fish fry at 60 days of age were selected, strontium chloride hexahydrate was added to the culture water to the target concentration (20 mg / L, 40 mg / L, 80 mg / L, 160 mg / L, 320 mg / L, 640 mg / L), the marking treatment was performed for 2 days (no feeding during the period, and the aquarium was maintained clean and oxygenated), and otolith analysis was performed after temporary culture for 10 days. Each concentration corresponds to an independent release batch.
[0027] Each release batch is marked with an independent strontium concentration (20mg / L, 40mg / L, 80mg / L, 160mg / L, 320mg / L, 640mg / L). After the reproduction and release, the distribution map of strontium in the otoliths is analyzed by electronic probe. Based on the peak position and intensity difference, the original release batch to which the fry belong can be accurately traced, thus realizing batch tracking and management.
[0028] Marking effect of strontium chloride hexahydrate at different concentration gradients on juvenile roundmouth copperfish
[0029] In this experiment, healthy round-mouthed copper fish fry artificially bred at the Xiangjiaba breeding and release station in Yibin City, Sichuan Province were selected. Six different concentration gradients (20mg / L, 40mg / L, 80mg / L, 160mg / L, 320mg / L, 640mg / L) and a control group (the control group did not add strontium chloride hexahydrate) were set up in the experiment. Each treatment group and control group had 10 samples.
[0030] The treated water was prepared with different concentration gradients by adding strontium chloride hexahydrate. The water source was tap water with an aeration time of not less than 2 days. Each treatment group and control group was equipped with an air stone to maintain the oxygen content of the water, and a filter was installed to ensure the water quality was clean. The experimental treatment time was 2 days, during which no feeding was carried out, and the number of deaths of fry was recorded every day.
[0031] After the otolith strontium element labeling experiment was completed, the treated round-mouthed copper fish in each group were moved to water without strontium chloride hexahydrate for temporary culture for 10 days. During this period, they were fed once every 24 hours according to 5% of the fish body weight. After the temporary culture, 5 fry were randomly selected from each group, their otoliths were collected and samples were prepared, and then electron probe analysis was performed to analyze the Sr element content in the samples.
[0032] Different concentration gradients of strontium chloride hexahydrate did not cause lethal effects on juvenile roundmouth copper fish. Figures 1-7 It shows that under different strontium concentration gradients, the otolith edge area forms specific peak marks (such as 20 mg / L corresponding to Figure 2 , 640mg / L corresponds to Figure 7 ), Figure 8Further verification shows that the differences in each concentration peak are significant and can be used as the unique fingerprint code for batches. These differentiated elemental fingerprints can effectively distinguish fry of different release batches, thus providing a reliable basis for subsequent evaluation of release effects and resource monitoring.
[0033] The above embodiments are only the preferred solutions of the present invention and do not constitute limitations in other forms of the present invention. After understanding the technical content of the present invention, any person skilled in the art can, without departing from the technical solution of the present invention, make appropriate changes or equivalent modifications to the embodiments. Any simple modification, equivalent change or modification made to the above embodiments without departing from the essence of the technical solution of the present invention shall fall within the protection scope of the technical solution of the present invention.
Claims
1. A method for distinguishing batches of round-mouthed copper fish proliferation and release based on strontium concentration gradient, characterized in that: The following steps are involved: Step (1) selecting healthy round-mouthed copper fish fry as the fry to be marked; Step (2) setting at least two different strontium concentration gradients, wherein the strontium concentration gradients are formed by strontium chloride hexahydrate SrCl2·6H2O in the culture water body, and the concentration range is between 20 mg / L and 640 mg / L; Step (3) placing the fry to be marked in culture water containing different strontium concentrations and immersing them respectively, so that each release batch corresponds to a unique strontium concentration mark; Step (4) uses electron probe analysis technology to perform strontium element distribution spectrum analysis on the marked fry otoliths, and uniquely identifies the original release batch to which the fry belong based on the peak position and intensity difference in the spectrum, thereby achieving accurate tracking and management of multiple batches.
2. The method for distinguishing batches of round-mouthed copper fish proliferation and release according to claim 1, characterized in that: The healthy round-mouthed copper fish fry described in step (1) are healthy round-mouthed copper fish fry that are 60 to 90 days old after seedling rearing.
3. The method for distinguishing batches of round-mouthed copper fish proliferation and release according to claim 1, characterized in that: The strontium concentration gradient in step (2) is set to discrete concentration values of 20 mg / L, 40 mg / L, 80 mg / L, 160 mg / L, 320 mg / L and 640 mg / L.
4. The method for distinguishing batches of round-mouthed copper fish proliferation and release according to claim 1, characterized in that: Step (3), during the soaking treatment, the fry are not fed and the culture water is kept clean and oxygen-rich.
5. The method for distinguishing batches of round-mouthed copper fish proliferation and release according to claim 1, characterized in that: Step (3), the soaking time is 2 days.
6. The method for distinguishing batches of round-mouthed copper fish proliferation and release according to claim 1, 2, 3, 4 or 5, characterized in that: After the soaking treatment is completed, the fry are moved to a water environment without strontium chloride hexahydrate for a period of time. Normal feeding is carried out during the temporary rearing period, and the temporary rearing time is not less than 10 days.
7. Application of the method for distinguishing batches of round-mouthed copper fish proliferation and release according to any one of claims 1 to 6 in the proliferation and release of multiple batches of round-mouthed copper fish.