Monoclonal antibody against chinese shrimp hemocyanin and its application

CN120349408BActive Publication Date: 2026-05-29SHANGHAI EPIZYME BIOMEDICAL TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510343059.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-30
Publication Date
2026-05-29
Estimated Expiration
2044-10-30

AI Technical Summary

Technical Problem

In existing technologies, monoclonal antibodies against hemocyanin in Chinese shrimp are not expressed stably in hybridoma cells, making it difficult to effectively detect and monitor fluctuations in hemocyanin concentration.

Method used

The monoclonal antibody anti-SHC-5C8 against hemocyanin of Chinese shrimp was stably expressed in mammalian cells Expi293. It was expressed and purified in E. coli DH5α and Expi293 cells using the recombinant plasmid pcDNA3.1 to ensure the high specificity and stability of the antibody.

Benefits of technology

Stable expression and high-purity purification of monoclonal antibody against hemocyanin in Chinese shrimp were achieved in Expi293 cells. The antibody exhibits good specificity and can be used as a positive internal control for qualitative and quantitative detection of hemocyanin immune status.

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Abstract

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.
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Citation Information

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