An oral vaccine containing a largemouth bass iridovirus ATPase and its preparation method and application

By constructing a recombinant vaccine that displays the ATPase of largemouth bass iridovirus on the surface of Bacillus subtilis, the problems of operational complexity and short duration of immunity of existing LMBV vaccines have been solved, and a highly efficient immune protection effect has been achieved.

CN121714685BActive Publication Date: 2026-05-29JIMEI UNIV
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Patent Information

Application Number
CN202610226979.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-02-26
Publication Date
2026-05-29
Estimated Expiration
2046-02-26

AI Technical Summary

Technical Problem

Existing LMBV vaccines suffer from problems such as complex operation, high cost, short duration of immunity, and difficulty in large-scale promotion. Moreover, most existing oral vaccines focus on the MCP monoantigen, and no vaccines have been found to be prepared using LMBV ATPase.

Method used

Using Bacillus subtilis as a vector and CotC, a spore capsid protein, as an anchoring protein, the ATPase of largemouth bass iridovirus was fused with the CotC gene to construct a recombinant spore surface display system, which was then used to prepare an oral vaccine. This achieved tolerable delivery of ATPase antigen in the gastrointestinal environment and immune stimulation of gut-associated lymphoid tissue.

Benefits of technology

It achieved effective control of LMBV, significantly improved the immune protection rate of fish, stimulated the expression of immune-related genes, and reduced the histopathological damage caused by viral infection.

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Abstract

The present application belongs to the field of fish molecular immunology, and particularly relates to an oral vaccine containing a largemouth bass iridovirus ATPase and a preparation method thereof. B. subtilis The spores of the WB600 strain are antigen delivery carriers, the spore coat protein C (CotC) is used as an anchor protein, and the key protein of the largemouth bass iridovirus is fused with the ATPase gene and CotC the gene to construct a recombinant spore surface display system, so that the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. The vaccine can tolerate the gastrointestinal environment after being orally administered to fish, and is accurately delivered to the gut-associated lymphoid tissue to stimulate the immunity of the fish, so that the prevention and control of the largemouth bass iridovirus is finally achieved.
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