Acne-removing skin-care conditioning oil and preparation method thereof

Abstract

The acne-removing skin-care conditioning oil is prepared from the following components in parts by weight: 90 to 95 parts of sweet almond oil, 0.5 to 1.5 parts of ginger root oil, 0.5 to 1.5 parts of peppermint oil, 1.5 to 2.5 parts of lemon peel oil, 1 to 1.5 parts of eucalyptus globulus leaf oil and 2 to 3 parts of lemongrass leaf oil. The invention also discloses a preparation method of the acne-removing skin-care conditioning oil. The acne-removing skin-care conditioning oil disclosed by the invention is small in skin irritation, wide in universality and few in allergic phenomenon, and can also achieve the effects of repairing and skin care while removing acnes.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to an anti-acne skin care conditioning oil and a preparation method thereof. Background Art

[0002] Acne, blackheads, and pimples, collectively known as acne, is a common chronic disease of the sebaceous glands. Acne is often caused by endocrine imbalances. While conventional Chinese and Western medicines can address endocrine function, they are typically taken orally, rarely used topically. They also suffer from slow treatment, limited effectiveness, and the development of drug resistance. Existing acne-removing cosmetics are mostly chemically synthesized, irritating to the skin, leaving severe scars, and limited universal applicability. Some products can even cause allergic reactions. Summary of the Invention

[0003] The purpose of the present invention is to address the above-mentioned deficiencies and provide an acne-removing skin care conditioning oil and a preparation method thereof.

[0004] The acne-removing skin-care conditioning oil of the present invention comprises the following components in parts by weight: 90-95 parts sweet almond oil 0.5-1.5 parts ginger root oil 0.5-1.5 parts peppermint oil 1.5-2.5 parts lemon peel oil 1-1.5 parts eucalyptus globulus leaf oil 2-3 parts lemongrass leaf oil

[0005] Preferably, the following components are included in parts by weight: 92.73 parts of sweet almond oil 0.91 parts ginger root oil 0.91 parts peppermint oil 1.82 parts lemon peel oil 1.36 parts eucalyptus globulus leaf oil 2.27 parts lemongrass leaf oil.

[0006] Preferably, the following components in parts by weight are further included: 2-4 parts of zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid combination.

[0007] Preferably, the preparation steps of the zinc gluconate / oil-soluble fullerene / hydrolyzed hyaluronic acid compound are: T1. Weigh zinc gluconate and hydrolyzed hyaluronic acid in a mass ratio of (3-5):2, add deionized water, and stir at 40-50°C and 200-400 rpm until the zinc gluconate and hydrolyzed hyaluronic acid are completely dissolved to form a clear and transparent solution; T2. Add half the total weight of the alkylated fullerene, which accounts for half of the total weight of the zinc gluconate and hydrolyzed hyaluronic acid, to anhydrous ethanol. Place the mixture in an ultrasonicator and set the ultrasonic power to 200-500W for 15-30 minutes to uniformly disperse the fullerene in the ethanol to form a stable fullerene dispersion. T3. While stirring, add the fullerene dispersion to the clear transparent solution of T1 and continue stirring for 30-45 minutes to mix the two evenly to form a mixed solution. The mixture is then filtered and dried to obtain a zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0008] Preferably, the alkylated fullerene is any one of methylated fullerene, ethylated fullerene, dodecylated fullerene and hexadecyl fullerene.

[0009] A method for preparing an acne-removing skin-care conditioning oil comprises the following steps: S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Taking the sweet almond oil in parts by weight as the base oil, and then sequentially adding the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, lemongrass leaf oil and the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound in parts by weight prepared in S3, and then performing high-pressure homogenization treatment in a high-pressure homogenizer to prepare the anti-acne skin care conditioning oil.

[0010] Preferably, in the S4 high-pressure homogenization treatment, the initial pressure is set at 20-50 MPa, the final pressure is set at 80-150 MPa, the number of cycles is 5-8 times, the homogenization temperature is 20-30° C., and the flow rate is 50-100 L / h.

[0011] Preferably, in the S4 high-pressure homogenization treatment, the initial pressure is set at 30 MPa, the final pressure is set at 120 MPa, the number of cycles is 6, the homogenization temperature is room temperature (25° C.), and the flow rate is 60 L / h.

[0012] Preferably, when the entire distillation pipeline is sterilized at high temperature in S2, steam is sterilized at high temperature in the entire distillation pipeline for at least 10 minutes.

[0013] Sweet almond oil is rich in unsaturated fatty acids, such as oleic and linoleic acid, which replenish moisture and nutrients, leaving skin soft and smooth. For acne-prone skin, it can improve dryness without adding excess oil, thereby preventing the imbalance in oil secretion caused by excessive dryness and indirectly preventing breakouts. Its gentle properties are suitable for all skin types, including sensitive and acne-prone skin, and can reduce the risk of skin allergies.

[0014] Gingerol and zingiberene, among other ingredients in ginger root oil, have antibacterial and anti-inflammatory properties. They can inhibit the growth of acne-causing bacteria like Propionibacterium acnes, reduce inflammation, and alleviate acne redness and swelling. It also promotes blood circulation in the skin, helping to deliver more nutrients to skin cells, boosting skin metabolism, helping to repair damaged skin, and accelerating acne healing.

[0015] Peppermint oil, whose main ingredient, menthol, imparts a cooling sensation, can reduce the dryness and inflammation of acne-prone skin, alleviating redness, swelling, and pain. Menthol also has antibacterial properties, inhibiting bacterial growth and reducing the risk of breakouts. Peppermint oil can also shrink pores, reducing oil secretion and the chance of pore clogging, thereby preventing acne from occurring at its root.

[0016] Lemon peel oil, rich in ingredients like limonene, has antibacterial properties that purify the skin's surface and inhibit acne-causing bacteria. Its refreshing scent also helps reduce skin odor. Certain components in lemon peel oil also have brightening properties, reducing pigmentation left behind after acne breakouts and promoting a more even complexion.

[0017] Eucalyptus globulus leaf oil contains ingredients such as eucalyptol, which have strong antibacterial properties and can effectively inhibit various bacteria such as Propionibacterium acnes. It has a good therapeutic effect on inflammatory acne. Its anti-inflammatory properties can reduce the inflammatory response of acne, reduce redness and swelling, and promote the disappearance of acne.

[0018] The antibacterial and insect repellent properties of lemongrass leaf oil, such as citronellal and geraniol, not only inhibit acne-causing bacteria but also prevent insect bites from causing secondary damage to affected skin. It soothes the skin, reduces the itching and discomfort associated with acne, and calms the skin, helping it return to a calm state.

[0019] Advantages of the present invention: Zinc gluconate and hydrolyzed hyaluronic acid are insoluble in oils, but when encapsulated or adsorbed with alkylated fullerenes, they can be effectively dispersed in essential oils, gradually releasing their respective effects. When zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid is added to acne-fighting essential oils, the three ingredients work synergistically in multiple ways, achieving superior acne-fighting and skincare results: Synergistic effect targeting the root cause of acne Combining oil secretion regulation with antioxidant properties: Acne is closely linked to excessive oil secretion from the sebaceous glands. Oxidative stress can also exacerbate skin inflammation and contribute to acne formation. Zinc gluconate participates in the synthesis and activation of various enzymes in the skin, regulating sebaceous cell metabolism and thereby reducing oil secretion. Alkylated fullerenes, with their potent antioxidant properties, scavenge excess free radicals in the skin and reduce oxidative stress. These two ingredients work synergistically to reduce oil secretion at the source while improving the skin's oxidative environment and reducing the risk of acne.

[0020] Anti-inflammatory, antibacterial, and cell repair: Propionibacterium acnes infection is a major cause of acne, and inflammation can exacerbate acne symptoms. Zinc gluconate has certain antibacterial and anti-inflammatory properties, inhibiting the growth and reproduction of P. acnes and reducing inflammation. Alkylated fullerenes not only have antioxidant properties but also promote skin cell metabolism and repair. Working together, they fight bacterial infection, reduce inflammation, repair damaged skin cells, and accelerate acne healing.

[0021] Synergistically improve the overall condition of the skin Hydrating and Repairing Synergy: Hydrolyzed hyaluronic acid possesses excellent moisturizing properties, forming a hydrating film on the skin's surface, increasing moisture and maintaining hydration. A good hydrating environment is crucial for the repair of acne-prone skin. While zinc gluconate and alkylated fullerenes promote skin cell repair and regeneration, the moisturizing environment provided by hydrolyzed hyaluronic acid creates favorable conditions for cell repair and regeneration, keeping the skin healthy while combating acne and reducing skin problems caused by dryness.

[0022] Maintaining skin barrier function: Impaired skin barrier function reduces skin's resistance to external stimuli, making acne more likely to occur. Zinc gluconate helps maintain the skin's normal structure and function, strengthening the integrity of the skin barrier. The antioxidant properties of alkylated fullerenes protect skin cells from free radical damage, further maintaining the skin barrier. The moisturizing properties of hydrolyzed hyaluronic acid keep the skin barrier soft and elastic. These three ingredients work together to maintain skin barrier function, enhance skin resistance, and prevent the recurrence of acne.

[0023] Improve the synergy between product stability and security Enhanced Product Stability: The presence of alkylated fullerenes improves the stability of acne-fighting essential oils, protecting them from damage to the active ingredients in the oils by environmental factors (such as oxidation and light). Zinc gluconate and hydrolyzed hyaluronic acid also protect other essential oil components to a certain extent, reducing their oxidation and degradation. These three factors work together to extend the product's shelf life and ensure its continued effectiveness.

[0024] Reduces product irritation: Some acne-fighting essential oils may contain irritants that can easily cause skin allergies or discomfort. Zinc gluconate and hydrolyzed hyaluronic acid soothe the skin, protecting the skin barrier and reducing the damage caused by these irritants. The antioxidant properties of alkylated fullerenes also help reduce inflammation caused by external irritants. These three ingredients work together to make the product safer and gentler, suitable for a wide range of skin types. DETAILED DESCRIPTION

[0025] <Example 1> An acne-removing skin-care conditioning oil comprises the following components in parts by weight: 92.73 parts of sweet almond oil 0.91 parts ginger root oil 0.91 parts peppermint oil 1.82 parts lemon peel oil 1.36 parts eucalyptus globulus leaf oil 2.27 parts lemongrass leaf oil.

[0026] A method for preparing an acne-removing skin-care conditioning oil comprises the following steps: S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus globulus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Take the sweet almond oil in parts by weight as the base oil, add the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, and lemongrass leaf oil in parts by weight in S3, and mix evenly to obtain the anti-acne skin care conditioning oil.

[0027] <Example 2> The acne-removing skin-care conditioning oil of the present invention comprises the following components in parts by weight: 90-95 parts sweet almond oil 0.5 parts ginger root oil 0.5 parts peppermint oil 1.5 parts lemon peel oil 1 part eucalyptus globulus leaf oil 2 parts lemongrass leaf oil 2 parts of zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0028] The preparation steps of the zinc gluconate / oil-soluble fullerene / hydrolyzed hyaluronic acid compound are as follows: T1. Weigh zinc gluconate and hydrolyzed hyaluronic acid in a mass ratio of 3:2, add deionized water, and stir at 40°C and 200 rpm until the zinc gluconate and hydrolyzed hyaluronic acid are completely dissolved to form a clear and transparent solution; T2. Add half the total weight of the alkylated fullerene, which accounts for half of the total weight of the zinc gluconate and hydrolyzed hyaluronic acid, to anhydrous ethanol. Place the mixture in an ultrasonicator and set the ultrasonic power to 200 W for 15 minutes to uniformly disperse the fullerene in the ethanol to form a stable fullerene dispersion. T3. While stirring, add the fullerene dispersion to the clear transparent solution of T1 and continue stirring for 30 minutes to mix the two evenly to form a mixed solution. The mixture is then filtered and dried to obtain a zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0029] The alkylated fullerene is a methylated fullerene.

[0030] S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Taking the sweet almond oil in parts by weight as the base oil, and then sequentially adding the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, lemongrass leaf oil and the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound in parts by weight prepared in S3, and then performing high-pressure homogenization treatment in a high-pressure homogenizer to prepare the anti-acne skin care conditioning oil.

[0031] In the S4 high-pressure homogenization treatment, the initial pressure was set at 20 MPa, and the final pressure was set at 80 MPa; the number of cycles was 5, the homogenization temperature was 20°C, and the flow rate was 50 L / h.

[0032] When the entire distillation pipeline is sterilized at high temperature in S2, steam is sterilized at high temperature in the entire distillation pipeline for 10 minutes.

[0033] <Example 3> The acne-removing skin-care conditioning oil of the present invention comprises the following components in parts by weight: 95 parts of sweet almond oil 1.5 parts ginger root oil 1.5 parts peppermint oil 2.5 parts lemon peel oil 1.5 parts eucalyptus globulus leaf oil 3 parts lemongrass leaf oil 4 parts of zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid combination.

[0034] The preparation steps of the zinc gluconate / oil-soluble fullerene / hydrolyzed hyaluronic acid compound are as follows: T1. Weigh zinc gluconate and hydrolyzed hyaluronic acid in a mass ratio of 2:1, add deionized water, and stir at 400 rpm at 50°C until the zinc gluconate and hydrolyzed hyaluronic acid are completely dissolved to form a clear and transparent solution; T2. Add half the total weight of the alkylated fullerene, which accounts for half of the total weight of the zinc gluconate and hydrolyzed hyaluronic acid, to anhydrous ethanol. Place the mixture in an ultrasonicator and set the ultrasonic power to 500 W for 30 minutes to uniformly disperse the fullerene in the ethanol to form a stable fullerene dispersion. T3. While stirring, add the fullerene dispersion to the clear transparent solution of T1 and continue stirring for 45 minutes to mix the two evenly to form a mixed solution. The mixture is then filtered and dried to obtain a zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0035] The alkylated fullerene is hexadecyl fullerene.

[0036] A method for preparing an acne-removing skin-care conditioning oil comprises the following steps: S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Taking the sweet almond oil in parts by weight as the base oil, and then sequentially adding the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, lemongrass leaf oil and the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound in parts by weight prepared in S3, and then performing high-pressure homogenization treatment in a high-pressure homogenizer to prepare the anti-acne skin care conditioning oil.

[0037] In the S4 high-pressure homogenization treatment, the initial pressure was set at 50 MPa and the final pressure was set at 150 MPa; the number of cycles was 8 times, the homogenization temperature was 30°C, and the flow rate was 100 L / h.

[0038] When the entire distillation pipeline is sterilized at high temperature in S2, steam is sterilized at high temperature in the entire distillation pipeline for 20 minutes.

[0039] <Example 4> The acne-removing skin-care conditioning oil of the present invention comprises the following components in parts by weight: 92.73 parts of sweet almond oil 0.91 parts ginger root oil 0.91 parts peppermint oil 1.82 parts lemon peel oil 1.36 parts eucalyptus globulus leaf oil 2.27 parts lemongrass leaf oil 3 parts of zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0040] The preparation steps of the zinc gluconate / oil-soluble fullerene / hydrolyzed hyaluronic acid compound are as follows: T1. Weigh zinc gluconate and hydrolyzed hyaluronic acid in a mass ratio of 2:1, add deionized water, and stir at 45°C and 300 rpm until the zinc gluconate and hydrolyzed hyaluronic acid are completely dissolved to form a clear and transparent solution; T2. Add half the total weight of the alkylated fullerene, which accounts for half of the total weight of the zinc gluconate and hydrolyzed hyaluronic acid, to anhydrous ethanol. Place the mixture in an ultrasonicator and set the ultrasonic power to 300 W for 20 minutes to uniformly disperse the fullerene in the ethanol to form a stable fullerene dispersion. T3. While stirring, add the fullerene dispersion to the clear transparent solution of T1 and continue stirring for 40 minutes to mix the two evenly to form a mixed solution. The mixture is then filtered and dried to obtain a zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

[0041] The alkylated fullerene is an ethylated fullerene.

[0042] <Solubility Test> Experimental materials and equipment Materials: alkylated fullerene (ethylated fullerene, purity 99%), zinc gluconate (analytical grade), hydrolyzed hyaluronic acid (molecular weight less than 10,000 Da, purity 98%), sweet almond oil, deionized water, and anhydrous ethanol (analytical grade).

[0043] Equipment: electronic balance (accuracy 0.0001g), magnetic stirrer (speed range 0-2000r / min), ultrasonic cleaner (power 100-500W, frequency 40-80kHz), centrifuge (speed range 0-10000r / min), oven, etc.

[0044] Experimental steps and parameters Preparation of stock solution Zinc gluconate stock solution: Accurately weigh 2.0000g of zinc gluconate, dissolve it in deionized water, and dilute to 100mL to obtain a stock solution with a concentration of C01 = 20mg / mL. Hydrolyzed hyaluronic acid stock solution: Weigh 1.0000g of hydrolyzed hyaluronic acid, add appropriate amount of deionized water, stir and dissolve at 40°C for 2 hours, cool, and dilute to 100mL to obtain a stock solution with a concentration of C02 = 10mg / mL. Alkylated fullerene-oil solution: Weigh 0.3000 g of alkylated fullerene (tests show that the mass of ethylated fullerene that can be dissolved in every 100 g of sweet almond oil at room temperature is approximately between 0.3 and 0.5 g), add 10 mL of anhydrous ethanol to assist dissolution, and then slowly add it to 90 g of sweet almond oil. Stir on a magnetic stirrer for 1 hour (speed 500 rpm) while evaporating the anhydrous ethanol in a fume hood to obtain an alkylated fullerene-sweet almond oil solution with a concentration of CF = 3 mg / g.

[0045] Solubility experiment setup Control group: 50 g of sweet almond oil was placed in a 100 mL conical flask, 5 mL of zinc gluconate stock solution and 3 mL of hydrolyzed hyaluronic acid stock solution were added, and the mixture was stirred at 300 r / min at 25°C for 2 hours. The mixture was then placed in an ultrasonic cleaner and ultrasonically treated at a power of 200 W and a frequency of 60 kHz for 30 minutes. The mixture was then allowed to stand for 24 hours.

[0046] Experimental group: 50 g of alkylated fullerene-sweet almond oil solution was placed in a 100 mL conical flask, and the same volume of zinc gluconate stock solution and hydrolyzed hyaluronic acid stock solution as the control group was added. The same stirring, ultrasonication and standing procedures as the control group were repeated.

[0047] Sample processing After standing, the samples from the experimental and control groups were transferred to centrifuge tubes, centrifuged at 5000 rpm for 15 minutes, and then filtered and dried to obtain the insoluble matter. The total mass of the insoluble matter in the experimental group was m2 = 0.0695 g, and the total mass of the insoluble matter in the control group was m1 = 0.1262 g. As can be seen, the total mass of the insoluble matter in the experimental group was significantly lower than that in the control group. Therefore, zinc gluconate and hydrolyzed hyaluronic acid, which are poorly soluble in oils and fats, can achieve partial affinity with oils and fats with the help of alkylated fullerenes, thereby improving their solubility in oils and fats.

[0048] This shows that in the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound, alkylated fullerene also has the effect of promoting the dissolution of zinc gluconate and hydrolyzed hyaluronic acid in oils and fats.

[0049] A method for preparing an acne-removing skin-care conditioning oil comprises the following steps: S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Taking the sweet almond oil in parts by weight as the base oil, and then sequentially adding the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, lemongrass leaf oil and the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound in parts by weight prepared in S3, and then performing high-pressure homogenization treatment in a high-pressure homogenizer to prepare the anti-acne skin care conditioning oil.

[0050] In the S4 high-pressure homogenization treatment, the initial pressure was set at 30 MPa, and the final pressure was set at 120 MPa; the number of cycles was 6, the homogenization temperature was room temperature (25°C), and the flow rate was 60 L / h.

[0051] When the entire distillation pipeline is sterilized at high temperature in S2, steam is sterilized at high temperature in the entire distillation pipeline for 15 minutes.

[0052] Analysis and testing <Skin test> 1. Inspection purpose Qualified subjects were selected according to the subject inclusion and exclusion criteria to conduct human trial testing on the product of Example 4. The corresponding parameters of the subjects' facial skin were compared before and after through researcher evaluation, image acquisition, and instrument measurement, and the efficacy and safety of the test product were clinically evaluated.

[0053] 2. Subject screening criteria 2.1. Inclusion criteria 2.1.1. Applicants must be healthy women or men aged between 18 and 40 years old; 2.1.2. Those with a certain amount of acne or pimples on their facial skin; 2.1.3. No allergic diseases, no history of allergies to cosmetics or other external use preparations; 2.1.4. The facial skin should be free of birthmarks, scratches, white spots, pigmented moles, and other phenomena that may affect the test; 2.1.5. Be able to read Chinese and accurately understand the trial procedures, voluntarily participate in the trial and sign the Informed Consent Form; 2.1.6. Be able to cooperate with the return visits of the trial study participants and promptly report any changes in their health status or medications, adverse reactions, and comply with the study requirements and schedule; 2.1.7. Do not use any other cosmetics, medicines or health products that may affect the results during the trial.

[0054] Exclusion criteria 2.2.1. Women who are planning to become pregnant, are pregnant, breastfeeding, or within six months after giving birth; 2.2.2. Those with severe systemic diseases, immunodeficiency or autoimmune diseases; 2.2.3. Those who have used antihistamines in the past week or immunosuppressants in the past month; 2.2.4. Those who have used any anti-inflammatory drugs in the test area in the past two months; 2.2.5. The test site has participated in other clinical trials in the past three months; 2.2.6. Patients with respiratory diseases who are receiving treatment; 2.2.7. People who are receiving dermatological treatment; 2.2.8. Patients with insulin-dependent diabetes mellitus.

[0055] 2.3. Exit Criteria During the trial, if the subject becomes pregnant, experiences adverse reactions, is lost to follow-up without reason, or violates the study protocol (such as using other cosmetics or drugs that may affect the study results) or other special reasons, the researcher will terminate her / his participation in the study.

[0056] 3. Subject Information After screening by dermatologists, a total of 30 healthy subjects were selected, and ultimately 30 subjects completed the trial, including 8 males and 22 females.

[0057]

[0058] 4. Inspection methods Environmental conditions Temperature 21±1℃, relative humidity 50±10%.

[0059] Evaluation methods

[0060] 4.3. Safety and adverse reaction evaluation During the trial, adverse reactions were observed and evaluated three times: at D0, D7, and D14 follow-up visits. A professional dermatologist asked participants whether they experienced facial skin symptoms such as itching, burning, tightness, dryness, scaling, stinging, or redness while using the test product. The presence of rash, redness, swelling, or scaling was also recorded. Scoring was then performed according to the criteria in Table 1. The scores were recorded on the case report form and evaluated for relevance using the criteria listed in Table 2.

[0061] Table 1 Scoring criteria for adverse reactions

[0062] Data Analysis Data were analyzed using SPSS 26.0 statistical software. Paired t-tests or rank-sum tests were used to analyze differences between baseline and follow-up values for each parameter. "ns" indicates no statistically significant difference, and "*," "**," and "***" indicate P < 0.05, P < 0.01, and P < 0.001, respectively.

[0063] 4.5. Basis for determination Based on the comparison of the baseline value (DO) of each parameter and the values at different revisit time points (D7 and D14), if the change in any parameter of the visual assessment or image analysis-related parameters at any visit time point before and after product use is significantly improved (P<0.05), it means that the test product has acne-removing efficacy; otherwise, the test product is considered to have no acne-removing efficacy.

[0064] 4.6. Facial Comedones Count Analysis Dermatologists counted the comedones on the subjects' faces. A smaller number indicates fewer comedones, and a higher number indicates more comedones.

[0065] 4.6.1. Analysis of facial comedones The results of facial acne counts at different visit points are as follows: Table 3 Difference analysis of facial comedones count

[0066] Summary Compared with baseline values (DO), facial comedones counts decreased by 27.16% and 43.77% at the D7 and D14 follow-up visits, respectively. Analysis of variance revealed significant differences in facial comedones counts compared with baseline values (DO) at both D7 and D14 follow-up visits (P < 0.001) (see Table 3).

[0067] 4.7. Skin a* value analysis 4.7.1. Result analysis The skin a* value is measured by taking standard images with VISIA-CR. Image analysis software is used to analyze the a* value of the skin at the test site. The smaller the a* value, the milder the acne inflammation, and vice versa.

[0068] The results of skin a* value detection at different return visit points are as follows: Table 4 Analysis of differences in skin a* values

[0069] Summary Compared to baseline values (D0), the skin a* values decreased by 6.44% and 11.36% at the D7 and D14 follow-up visits, respectively. Analysis of variance revealed significant differences in the skin a* values at the D7 and D14 follow-up visits compared to baseline values (D0) (D7: P < 0.05; D14: P < 0.01) (Table 4).

[0070] 4.8. Subject Self-Assessment Questionnaire After 2 weeks of product use, the results of the questionnaires filled in by the subjects were summarized and expressed as the percentage (%) of subjects who agreed with a certain answer.

[0071]

[0072] Summary After two weeks of use, 86.66% of participants responded positively to the "pore-tightening effect," 86.67% reported a reduction in acne, 90% reported a reduction in comedones (open and closed comedones), 93.33% reported oil control at the application site and improvement in redness at the application site, and 96.67% reported improvement in inflammation at the application site. 96.67% of participants rated the product's overall satisfaction rating at 4 out of 5 or higher.

[0073] 5. Conclusion A clinical efficacy trial of 30 subjects using acne-fighting skincare conditioning oil yielded the following results: During the trial, none of the 30 subjects experienced any adverse reactions.

[0074] Compared with baseline values (DO), facial comedonal counts decreased by 27.16% and 43.77% at the D7 and D14 follow-up visits, respectively. Significant differences were observed between the D7 and D14 follow-up visits and baseline values (D0) (P < 0.001). Skin a* values decreased by 6.44% and 11.36% at the D7 and D14 follow-up visits, respectively. Significant differences were observed between the D7 and D14 follow-up visits and baseline values (DO) (D7: P < 0.05; D14: P < 0.01).

[0075] After two weeks of use, 86.66% of participants responded positively to the "pore-tightening effect," 86.67% reported a reduction in acne, 90% reported a reduction in comedones (open and closed comedones), 93.33% reported oil control at the application site and improvement in redness at the application site, and 96.67% reported improvement in inflammation at the application site. 96.67% of participants rated the product's overall satisfaction rating at least 4 out of 5.

[0076] The test results showed that the facial acne counts and skin a* values of the 30 subjects were significantly improved when they were revisited on D7 and D14 compared with D0, and the test product had acne-removing and skin-care effects.

[0077] The above implementation cases are only for illustrating the technical solutions and features of the present invention, and their purpose is to enable people familiar with the technology to implement them better. They cannot be used to limit the scope of protection of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention are within the scope of protection of the present invention. The ones not described in detail are prior art.

Claims

1. An acne-removing skin-care conditioning oil, characterized in that: The composition comprises the following components in parts by weight: 90-95 parts sweet almond oil 0.5-1.5 parts ginger root oil 0.5-1.5 parts peppermint oil 1.5-2.5 parts lemon peel oil 1-1.5 parts eucalyptus globulus leaf oil 2-3 parts lemongrass leaf oil 2. The acne-removing skin-care conditioning oil according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 92.73 parts of sweet almond oil 0.91 parts ginger root oil 0.91 parts peppermint oil 1.82 parts lemon peel oil 1.36 parts eucalyptus globulus leaf oil 2.27 parts lemongrass leaf oil.

3. The anti-acne skin care conditioning oil according to claim 1, characterized in that: Also includes the following components in parts by weight: 2-4 parts of zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid combination.

4. The acne-removing skin-care conditioning oil according to claim 3, characterized in that: The preparation steps of the zinc gluconate / oil-soluble fullerene / hydrolyzed hyaluronic acid compound are as follows: T1. Weigh zinc gluconate and hydrolyzed hyaluronic acid in a mass ratio of (3-5):2, add deionized water, and stir at 40-50°C and 200-400 rpm until the zinc gluconate and hydrolyzed hyaluronic acid are completely dissolved to form a clear and transparent solution; T2. Add half the total weight of the alkylated fullerene, which accounts for half of the total weight of the zinc gluconate and hydrolyzed hyaluronic acid, to anhydrous ethanol. Place the mixture in an ultrasonicator and set the ultrasonic power to 200-500W for 15-30 minutes to uniformly disperse the fullerene in the ethanol to form a stable fullerene dispersion. T3. While stirring, add the fullerene dispersion to the clear transparent solution of T1 and continue stirring for 30-45 minutes to mix the two evenly to form a mixed solution. The mixture is then filtered and dried to obtain a zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid complex.

5. The acne-removing skin-care conditioning oil according to claim 3, characterized in that: The alkylated fullerene is any one of methylated fullerene, ethylated fullerene, dodecylated fullerene and hexadecyl fullerene.

6. The method for preparing an anti-acne skin care conditioning oil according to claim 3, characterized in that: The following steps are involved: S1. Wash and remove impurities from freshly picked ginger, mint, lemon peel, eucalyptus leaves, and lemongrass leaves respectively, then crush them with a machine and send them to the respective material delivery bins; S2. Before distilling the raw materials, the entire distillation equipment pipeline is sterilized at high temperature, and steam is sterilized at high temperature in the entire distillation equipment pipeline; After S3 and sterilization are completed, each material is put into the distillation equipment in sequence to obtain the primary essential oil of each component, and then the primary essential oil of each component in the oil-water separator is taken out and left to stand for 2h to take the upper essential oil. This method is repeated three times to obtain the high-purity essential oil of each component, and then filtered through an inorganic ceramic membrane device in sequence, and passed through a macroporous resin adsorption column to obtain high-purity ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil and lemongrass leaf oil respectively; S4. Taking the sweet almond oil in parts by weight as the base oil, and then sequentially adding the ginger root oil, peppermint oil, lemon peel oil, eucalyptus globulus leaf oil, lemongrass leaf oil and the zinc gluconate / alkylated fullerene / hydrolyzed hyaluronic acid compound in parts by weight prepared in S3, and then performing high-pressure homogenization treatment in a high-pressure homogenizer to prepare the anti-acne skin care conditioning oil.

7. The method for preparing an acne-removing skin-care conditioning oil according to claim 6, characterized in that: In the S4 high-pressure homogenization treatment, the initial pressure is set at 20-50 MPa, and the final pressure is set at 80-150 MPa; the number of cycles is 5-8 times, the homogenization temperature is 20-30°C, and the flow rate is 50-100 L / h.

8. The method for preparing an acne-removing skin-care conditioning oil according to claim 7, characterized in that: In the S4 high-pressure homogenization treatment, the initial pressure was set at 30 MPa, and the final pressure was set at 120 MPa; the number of cycles was 6 times, the homogenization temperature was room temperature (25°C), and the flow rate was 60 L / h.

9. The method for preparing an anti-acne skin care conditioning oil according to claim 6, characterized in that: When the entire distillation pipeline is sterilized at high temperature in S2, steam is sterilized at high temperature in the entire distillation pipeline for at least 10 minutes.

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