Anti-aging composition based on NK cell activity enhancement and preparation method thereof

Abstract

The invention relates to the technical field of skin repair. The invention aims to provide an anti-aging composition with enhanced NK cell activity. The anti-aging composition comprises a utility component and an auxiliary material component for regulating physical properties of the anti-aging composition, the effective component is prepared from the following raw materials in parts by weight: 1 to 2 parts of palmitoyl pentapeptide-4, 1 to 2 parts of acetyl hexapeptide-8, 3 to 5 parts of ginsenoside Rh2, 10 to 15 parts of curcumin, 0.5 part of interleukin-2, 10 to 15 parts of antioxidant and 10 to 15 parts of humectant. According to the anti-aging composition disclosed by the invention, all the components are reasonably proportioned, so that the activity of NK (Natural Killer) cells can be enhanced, and the anti-aging composition has multiple anti-aging effects of resisting wrinkles, resisting oxidation, preserving moisture, regulating immunity and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of skin repair, and in particular to an anti-aging composition based on enhanced NK cell activity and a preparation method thereof. Background Art

[0002] With aging, the number and activity of NK cells (natural killer cells) in the human body gradually decline, with their function significantly weakening after age 40, leading to a decrease in the efficiency of senescent cell clearance. NK cells, core members of the human innate immune system, can directly kill senescent cells, secrete interferon-gamma to deactivate their metabolic support, and recruit macrophages and other cells to collaborate in their clearance, playing a crucial role in anti-aging. In 2024, Wang Ben's team at the Second Affiliated Hospital of Zhejiang University School of Medicine constructed the chimeric peptide E16-uPA24, which targets uPAR (urokinase-type plasminogen activator receptor) on the surface of senescent cells and glutamate receptor 5 on the NK cell membrane. This peptide enhances NK cell recognition and clearance, potentially improving age-related diseases and combating natural aging. Furthermore, a research team at China Pharmaceutical University used cell transfusion therapy to reinfuse NK cells cultured and expanded in vitro into volunteers, successfully enhancing the immune system's ability to clear senescent cells and improving the proportion of senescent CD3+ T cells and multiple inflammatory markers.

[0003] While most current anti-aging products focus on traditional approaches like anti-oxidation and promoting collagen synthesis, anti-aging compositions containing peptides that enhance NK cell activity hold broad application prospects. They could offer a new approach to health and skin care for healthy individuals seeking to slow the aging process and improve bodily functions. Summary of the Invention

[0004] The purpose of the present invention is to provide an anti-aging composition based on enhanced NK cell activity and a preparation method thereof, which combines the enhancement of NK cell activity with active peptides and antioxidant ingredients. NK cells not only have immune surveillance functions, but can also regulate the skin microenvironment by secreting cytokines, thereby promoting the repair and renewal of skin cells. Active peptides work in conjunction with NK cell activators from the perspective of endogenous cell repair, and the combination achieves anti-aging effects.

[0005] To achieve the above-mentioned purpose of the invention, the technical solution adopted by the present invention is an anti-aging composition based on enhancing NK cell activity, comprising effective ingredients and auxiliary ingredients for adjusting the physical properties of the anti-aging composition; the effective ingredients include the following raw materials in parts by weight: 1-2 parts of palmitoyl pentapeptide-4, 1-2 parts of acetyl hexapeptide-8, 3-5 parts of ginsenoside Rh2, 10-15 parts of curcumin, 0.5 parts of interleukin-2, 10-15 parts of antioxidant, and 10-15 parts of moisturizer.

[0006] Preferably, the weight ratio of the active ingredient to the auxiliary ingredient is 2:98 to 5:95.

[0007] Preferably, the antioxidant is one or more of astaxanthin, vitamin E, vitamin C, butylated hydroxytoluene, and catechin.

[0008] Preferably, the moisturizing agent is one or more of sodium hyaluronate, glycerin, and propylene glycol.

[0009] Preferably, the preparation method of the anti-aging composition comprises mixing the components of the effective ingredients according to the ratio, and continuously stirring for 30-60 minutes in a sterile environment, at a stirring temperature of 20-25° C. and a stirring speed of 100-200 rpm, until the components are fully mixed and uniformly formed into the effective ingredients; after the effective ingredients and the auxiliary materials are stirred and uniformly mixed, they are homogenized in a homogenizer for 10-15 minutes to obtain the anti-aging composition.

[0010] The beneficial effects of the present invention are concentrated in the following aspects: the anti-aging composition can enhance NK cell activity through the rational proportion of each component, and has multiple anti-aging effects such as anti-wrinkle, anti-oxidation, moisturizing and immune regulation. Palmitoyl pentapeptide-4 and acetyl hexapeptide-8 can promote collagen synthesis, inhibit nerve signal transmission, and reduce wrinkle formation. Ginsenoside Rh2 and curcumin are the main ingredients that enhance NK cell activity. They use interleukin-2 as a synergistic biotransmitter for the two. The three work synergistically to enhance the efficacy of the ingredients and activate immune cells, jointly promoting the enhancement of NK cell activity, improving the body's immune surveillance function, and achieving anti-aging effects. Supplemented with antioxidants to synergistically enhance the antioxidant effect, moisturizers to maintain skin moisture and improve the dryness caused by aging, the various ingredients work synergistically to achieve anti-aging from multiple dimensions of anti-wrinkle, anti-oxidation, immunity, and moisturizing. DETAILED DESCRIPTION

[0011] The present invention is an anti-aging composition based on enhanced NK cell activity, including effective ingredients and auxiliary ingredients for adjusting the physical properties of the anti-aging composition; its effective ingredients can be widely used in various cosmetics, skin care products, and medicines, and the auxiliary ingredients are designed according to the product requirements (physical properties) of the anti-aging composition and can be developed into various products such as water, emulsions, and creams.

[0012] The anti-aging composition comprises the following raw materials by weight: 1-2 parts of palmitoyl pentapeptide-4, 1-2 parts of acetyl hexapeptide-8, 3-5 parts of ginsenoside Rh2, 10-15 parts of curcumin, 0.5 parts of interleukin-2, 10-15 parts of antioxidant, and 10-15 parts of moisturizer. Palmitoyl pentapeptide effectively stimulates collagen synthesis, while acetyl hexapeptide-8 reduces neurotransmitter release, thereby reducing wrinkle formation. Ginsenoside Rh2 and curcumin, as the main components that enhance NK cell activity, use interleukin-2 as a synergistic biotransmitter between the two. The three act synergistically, enhancing the efficacy of the ingredients and activating immune cells, thereby promoting enhanced NK cell activity and improving the body's immune surveillance function, achieving anti-aging effects. It has been proven that when ginsenoside Rh2 is used in combination with an active peptide composition (palmitoyl pentapeptide-4, acetyl hexapeptide-8), its overall enhancing effect on NK cell activity is more obvious than curcumin. However, compared with curcumin, it has a more significant price disadvantage. This product, by adding interleukin-2 and synergizing the activation efficiency of the two through the biotransmitter effect of interleukin-2, can achieve more ideal product effects at a lower cost.

[0013] Theoretically, the antioxidants described herein can be one or more of astaxanthin, vitamin E, vitamin C, butylated hydroxytoluene, and catechins, and the moisturizers can be one or more of sodium hyaluronate, glycerin, and propylene glycol. Alternatively, further screening and application can be performed from various existing antioxidants and moisturizers. The weight ratio of the active ingredient to the auxiliary ingredients in this product is between 2:98 and 5:95, meaning that the active ingredients account for between 2% and 5% of the total weight of the anti-aging composition. This provides excellent efficacy while maintaining controllable overall product costs and increasing market acceptance.

[0014] The preparation method is as follows: the components of the effective ingredients are mixed according to the ratio, and stirring is continued for 30-60 minutes in a sterile environment, at a stirring temperature of 20-25°C and a stirring speed of 100-200 rpm, until the components are fully mixed and uniformly formed into the effective ingredients; after the effective ingredients and auxiliary materials are stirred and uniformly mixed, they are homogenized in a homogenizer for 10-15 minutes to obtain the anti-aging composition.

[0015] The present invention will be further described below with reference to specific embodiments, but these examples do not limit the invention in any way. Unless otherwise noted, the raw materials and reagents used in the examples are commercially available. To ensure experimental consistency, the antioxidants and moisturizers used in the following examples and control examples were identical in composition and proportion, as were the excipients, to form the anti-aging emulsions. The active ingredient to excipient ratio was designed to be 3:97.

[0016] Example 1

[0017] Palmitoyl pentapeptide-4 2 parts, acetyl hexapeptide-8 2 parts, ginsenoside Rh2 5 parts, curcumin 15 parts, interleukin-2 0.5 parts, antioxidant 15 parts, moisturizer 15 parts.

[0018] Example 2

[0019] Palmitoyl pentapeptide-4 2 parts, acetyl hexapeptide-8 2 parts, ginsenoside Rh2 3 parts, curcumin 10 parts, interleukin-2 0.5 parts, antioxidant 15 parts, moisturizer 15 parts.

[0020] Comparative Example 1

[0021] 1 part of palmitoyl pentapeptide-4, 1 part of acetyl hexapeptide-8, 5 parts of ginsenoside Rh2, 0.5 parts of interleukin-2, 15 parts of antioxidants, 15 parts of moisturizers.

[0022] Comparative Example 2

[0023] 1 part of palmitoyl pentapeptide-4, 1 part of acetyl hexapeptide-8, 15 parts of curcumin, 0.5 parts of interleukin-2, 15 parts of antioxidants, and 15 parts of moisturizers.

[0024] Comparative Example 3

[0025] 2 parts of palmitoyl pentapeptide-4, 2 parts of acetyl hexapeptide-8, 5 parts of ginsenoside Rh2, 15 parts of curcumin, 15 parts of antioxidants, and 15 parts of moisturizers.

[0026] experiment

[0027] 1. Experimental Subjects

[0028] Sixty 12-month-old SD rats were selected. After shaving the 1 square centimeter area on the back of the model rats, 3% hydrogen peroxide solution was applied every morning and evening for 4 consecutive weeks to establish the aging model rats.

[0029] The aging model mice were randomly divided into 6 groups, with 10 mice in each group, namely:

[0030] Blank control group: no treatment;

[0031] Normal saline group: applied normal saline;

[0032] High-dose group: applied the anti-aging lotion of Example 1;

[0033] Low-dose group: applied the anti-aging lotion of Example 2;

[0034] No curcumin group: applied the anti-aging lotion of control example 1;

[0035] Group without ginsenoside Rh2: applied the anti-aging lotion of Control Example 2;

[0036] Interleukin-2-free group: applied the anti-aging lotion of Control Example 3;

[0037] 2. Experimental operation and cycle

[0038] Apply 3 drops of lotion, normal saline solution or no treatment in the morning and evening as required above for 12 weeks, and measure and record relevant indicators every week.

[0039] 3. Testing indicators and data

[0040] After 12 weeks, the model mice were killed and the back skin tissue was taken for testing.

[0041] (1) NK cell cytotoxicity assay

[0042] The lactate dehydrogenase (LDH) release assay was used to determine the cytotoxic activity of NK cells against senescent fibroblasts. The results are shown in Table 1.

[0043] Table 1

[0044] Group NK cell killing activity (%) Blank control group 24.2±2.4 Normal saline group 26.8±2.2 High-dose group 70.2±3.8 Low-dose group 61.6±2.3 No curcumin group 49.5±3.7 Group without ginsenoside Rh2 37.1±2.4 No interleukin-2 group 53.2±3.5

[0045] Note: *P<0.05 compared with the blank control group.

[0046] Table 1 shows that treatment with the anti-aging composition of the present invention significantly enhanced the targeted killing ability of NK cells against senescent fibroblasts, with the degree of enhancement showing a positive dose-dependent effect. Furthermore, the introduction of interleukin-2 fully exploited its biotransmitter function, synergizing the effects of ginsenoside Rh2 and curcumin.

[0047] (2) Skin histological indicators

[0048] The skin thickness and collagen content were measured, and the results are shown in Table 2.

[0049] Table 2

[0050] Group Skin thickness (um) Collagen content (ug / mg) Blank control group 105.1±4.8 22.7±2.1 Normal saline group 112.2±3.4 26.4±2.5 High-dose group 186.6±4.2 63.2±3.5 Low-dose group 168.3±2.3 49.5±4.2 No curcumin group 152.5±3.8 43.8±3.4 Group without ginsenoside Rh2 139.2±4.7 38.7±4.4 No interleukin-2 group 159.5±4.1 47.4±4.3

[0051] Note: *P<0.05 compared with the blank control group.

[0052] As can be seen from Table 2, the present invention is effective in increasing skin thickness and collagen content, significantly tightening the skin and reducing wrinkles, and can delay skin aging. By inhibiting senescent fibroblasts, it can effectively reduce their adverse effects on surrounding normal cells, facilitating the absorption of active peptides by skin cells.

[0053] Furthermore, while achieving the same effect, the dosage of the high-value ginsenoside Rh2 can be significantly reduced, achieving a good balance between cost and effect. The anti-aging composition of the present invention has excellent market prospects when added to various cosmetics, skin care products, and skin medicines.

Claims

1. An anti-aging composition based on enhancing NK cell activity, comprising a functional component and auxiliary components for adjusting the physical properties of the anti-aging composition; characterized in that: The effective ingredients include the following raw materials in parts by weight: 1-2 parts of palmitoyl pentapeptide-4, 1-2 parts of acetyl hexapeptide-8, 3-5 parts of ginsenoside Rh2, 10-15 parts of curcumin, 0.5 parts of interleukin-2, 10-15 parts of antioxidant, and 10-15 parts of moisturizer.

2. The anti-aging composition based on enhancing NK cell activity according to claim 1, characterized in that: The weight ratio of the active ingredient to the auxiliary ingredient is 2:98 to 5:

95.

3. The anti-aging composition based on enhancing NK cell activity according to claim 1, characterized in that: The antioxidant is one or more of astaxanthin, vitamin E, vitamin C, butylated hydroxytoluene, and catechin.

4. The anti-aging composition based on enhancing NK cell activity according to claim 1, characterized in that: The moisturizing agent is one or more of sodium hyaluronate, glycerin, and propylene glycol.

5. The method for preparing the anti-aging composition based on enhancing NK cell activity according to any one of claims 1 to 4, characterized in that: The components of the effective ingredients are mixed according to the ratio, and stirring is continued for 30-60 minutes in a sterile environment, at a stirring temperature of 20-25°C and a stirring speed of 100-200 rpm, until the components are fully mixed and uniformly formed into the effective ingredients; after the effective ingredients and auxiliary materials are stirred and uniformly mixed, they are homogenized in a homogenizer for 10-15 minutes to obtain the anti-aging composition.