Food-grade composite biological agent formula based on sterilization and application of food-grade composite biological agent formula in microbial control of fresh agricultural products

Abstract

The invention relates to the technical field of food preservation, and discloses a food-grade composite biological agent formula based on sterilization and application of the food-grade composite biological agent formula in microorganism control of fresh agricultural products. Comprising the following components in parts by weight: 60-80 parts of a sterilization component, 2-5 parts of a synergist, 1-4 parts of a pH regulator, 2-6 parts of an atomization auxiliary agent, 1-2 parts of glycerol and 5-10 parts of deionized water. The preparation method comprises the following steps: uniformly mixing the sterilization component and deionized water, stirring and dissolving, continuously adding glycerol, a synergist and a pH regulator, regulating the pH value, finally adding an atomization auxiliary agent, and uniformly mixing to obtain the food-grade composite biological agent formula based on sterilization. The food-grade composite biological agent formula based on atomization sterilization has a good sterilization effect.

Description

Technical Field

[0001] The present invention relates to the technical field of food preservation, in particular to a food-grade composite biological agent formula based on sterilization and its application in microbial control of fresh agricultural products. Background Art

[0002] Existing methods for killing microorganisms to preserve food present numerous challenges, including the conflict between the time-efficient demands of streamlined operations and the low efficiency of traditional sterilization, incomplete sterilization leading to bacterial reactivation, food safety concerns caused by residual chemical sterilization residues, and the high equipment costs, inflexible operation, and long duration of physical sterilization. Therefore, an efficient, safe, and flexible food-grade compound biological formulation and its application method are urgently needed to address these issues. Summary of the Invention

[0003] (1) Technical problems solved In view of the shortcomings of the existing technology, the present invention provides a food-grade composite biological agent formula based on sterilization and its application in the microbial control of fresh agricultural products, which has a good sterilization effect.

[0004] (2) Technical solution To achieve the above objectives, the present invention provides the following technical solution: a food-grade composite biological agent formula based on sterilization, comprising the following components by weight: 60-80 parts by weight of a bactericidal component, 2-5 parts by weight of a synergist, 1-4 parts by weight of a pH regulator, 2-6 parts by weight of an atomization aid, 1-2 parts by weight of propylene glycol, and 5-10 parts by weight of deionized water.

[0005] Furthermore, the bactericidal component includes 5000-50000 U lysozyme, 15-50 U lysostaphin, 0.5-5 mM potassium sorbate and 1-5 mM calcium propionate.

[0006] Furthermore, the enhancer includes 0.5-4 mM polyethylene glycol 6000 and 2-8 mM EDTA-2Na.

[0007] Furthermore, the pH regulator includes 1-5 mM disodium hydrogen phosphate and 0.05-0.5% anhydrous citric acid.

[0008] Furthermore, the atomization aid is propylene glycol.

[0009] Furthermore, the preparation method of the sterilization-based food-grade composite biological agent formula is: mixing the sterilization component and deionized water evenly, stirring to dissolve, continuing to add glycerol, enhancer and pH regulator, adjusting the pH, and finally adding an atomization aid, mixing evenly, to obtain a sterilization-based food-grade composite biological agent formula.

[0010] Furthermore, the pH is adjusted to 7.5-8.

[0011] Furthermore, the application of the method in the microbial control of fresh agricultural products.

[0012] (3) Beneficial technical effects 1. Synergistic effect of ingredients: Organic acids and biosurfactants in a specific ratio produce synergistic effects on membrane penetration.

[0013] Lysozyme hydrolyzes β-1,4 glycosidic bonds, destroying the peptidoglycan network structure, causing the bacterial cell wall to rupture and ultimately causing the bacteria to die due to osmotic pressure imbalance.

[0014] Lysostaphin hydrolyzes the pentapeptide bridge, destroying the structure of peptidoglycan, causing the bacterial cell wall to rupture and ultimately causing the bacteria to die due to osmotic pressure imbalance.

[0015] Potassium sorbate can inhibit the dehydrogenase activity in microbial cells, blocking the respiration and energy metabolism of microorganisms, causing the microbial cells to be unable to carry out normal metabolic activities and eventually die.

[0016] Calcium propionate can inhibit enzyme activity within microbial cells and block microbial metabolic activity. It can also damage the structure of microbial cell membranes, causing cell contents to leak and ultimately killing the microorganisms.

[0017] 2. Responsive sterilization: pH-sensitive ingredients automatically release active substances in microbial enrichment areas.

[0018] 3. Second-level mechanism of action: The atomized carrier carries nano-scale active ingredients to directly destroy the cell membrane potential. DETAILED DESCRIPTION Example 1

[0019] A food-grade composite biological agent formula based on sterilization includes the following components by weight: 60 parts by weight of a sterilizing component, 2 parts by weight of a synergist, 1 part by weight of a pH regulator, 2 parts by weight of an atomization aid, 1 part by weight of glycerol, and 5 parts by weight of deionized water.

[0020] The bactericidal components include 5000 U lysozyme, 15 U lysostaphin, 0.5 mM potassium sorbate and 1 mM calcium propionate.

[0021] The enhancer includes 0.5 mM polyethylene glycol 6000 and 2 mM EDTA-2Na.

[0022] The pH adjuster includes 1 mM disodium hydrogen phosphate and 0.05% anhydrous citric acid.

[0023] The atomization aid is propylene glycol.

[0024] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH adjuster are added, the pH is adjusted to 7.5, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula. Example 2

[0025] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 80 parts by weight of a sterilizing component, 5 parts by weight of a synergist, 4 parts by weight of a pH regulator, 6 parts by weight of an atomizing aid, 2 parts by weight of glycerol, and 10 parts by weight of deionized water.

[0026] The bactericidal components include 50000 U lysozyme, 50 U lysostaphin, 5 mM potassium sorbate and 5 mM calcium propionate.

[0027] The enhancer includes 4 mM polyethylene glycol 6000 and 8 mM EDTA-2Na.

[0028] The pH adjuster includes 5 mM disodium hydrogen phosphate and 0.5% anhydrous citric acid.

[0029] The atomization aid is propylene glycol.

[0030] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 8, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula. Example 3

[0031] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 70 parts by weight of a sterilizing component, 4 parts by weight of a synergist, 3 parts by weight of a pH regulator, 5 parts by weight of an atomizing aid, 1.5 parts by weight of glycerol, and 8 parts by weight of deionized water.

[0032] The bactericidal components include 30,000 U lysozyme, 35 U lysostaphin, 2 mM potassium sorbate and 3 mM calcium propionate.

[0033] The enhancer includes 2 mM polyethylene glycol 6000 and 4 mM EDTA-2Na.

[0034] The pH adjuster includes 3 mM disodium hydrogen phosphate and 0.2% anhydrous citric acid.

[0035] The atomization aid is propylene glycol.

[0036] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 7.6, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula. Example 4

[0037] A food-grade composite biological agent formula based on sterilization includes the following components by weight: 60 parts by weight of a sterilizing component, 2 parts by weight of a synergist, 1 part by weight of a pH regulator, 2 parts by weight of an atomization aid, 1 part by weight of glycerol, and 5 parts by weight of deionized water.

[0038] The bactericidal components include 5000 U lysozyme, 15 U lysostaphin, 0.5 mM potassium sorbate and 1 mM calcium propionate.

[0039] The enhancer includes 0.5 mM polyethylene glycol 6000 and 2 mM EDTA-2Na.

[0040] The pH adjuster includes 5 mM disodium hydrogen phosphate and 0.5% anhydrous citric acid.

[0041] The atomization aid is propylene glycol.

[0042] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 7.6, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula. Example 5

[0043] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 80 parts by weight of a sterilizing component, 5 parts by weight of a synergist, 4 parts by weight of a pH regulator, 6 parts by weight of an atomizing aid, 2 parts by weight of glycerol, and 10 parts by weight of deionized water.

[0044] The bactericidal components include 50000 U lysozyme, 50 U lysostaphin, 5 mM potassium sorbate and 5 mM calcium propionate.

[0045] The enhancer includes 4 mM polyethylene glycol 6000 and 8 mM EDTA-2Na.

[0046] The pH adjuster includes 3 mM disodium hydrogen phosphate and 0.2% anhydrous citric acid.

[0047] The atomization aid is propylene glycol.

[0048] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH adjuster are added, the pH is adjusted to 7.5, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula. Example 6

[0049] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 70 parts by weight of a sterilizing component, 4 parts by weight of a synergist, 3 parts by weight of a pH regulator, 5 parts by weight of an atomizing aid, 1.5 parts by weight of glycerol, and 8 parts by weight of deionized water.

[0050] The bactericidal components include 30,000 U lysozyme, 35 U lysostaphin, 2 mM potassium sorbate and 3 mM calcium propionate.

[0051] The enhancer includes 2 mM polyethylene glycol 6000 and 4 mM EDTA-2Na.

[0052] The pH adjuster includes 1 mM disodium hydrogen phosphate and 0.05% anhydrous citric acid.

[0053] The atomization aid is propylene glycol.

[0054] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 8, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula.

[0055] Comparative Example 1

[0056] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 70 parts by weight of a sterilizing component, 4 parts by weight of a synergist, 3 parts by weight of a pH regulator, 5 parts by weight of an atomizing aid, 1.5 parts by weight of glycerol, and 8 parts by weight of deionized water.

[0057] The bactericidal components include 35 U of lysostaphin, 2 mM potassium sorbate and 3 mM calcium propionate.

[0058] The enhancer includes 2 mM polyethylene glycol 6000 and 4 mM EDTA-2Na.

[0059] The pH adjuster includes 1 mM disodium hydrogen phosphate and 0.05% anhydrous citric acid.

[0060] The atomization aid is propylene glycol.

[0061] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 8, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula.

[0062] Comparative Example 2

[0063] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 70 parts by weight of a sterilizing component, 4 parts by weight of a synergist, 3 parts by weight of a pH regulator, 5 parts by weight of an atomizing aid, 1.5 parts by weight of glycerol, and 8 parts by weight of deionized water.

[0064] The bactericidal components include 30,000 U lysozyme, 2 mM potassium sorbate and 3 mM calcium propionate.

[0065] The enhancer includes 2 mM polyethylene glycol 6000 and 4 mM EDTA-2Na.

[0066] The pH adjuster includes 1 mM disodium hydrogen phosphate and 0.05% anhydrous citric acid.

[0067] The atomization aid is propylene glycol.

[0068] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 8, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula.

[0069] Comparative Example 3

[0070] A food-grade composite biological agent formula based on sterilization comprises the following components by weight: 70 parts by weight of a sterilizing component, 4 parts by weight of a synergist, 3 parts by weight of a pH regulator, 5 parts by weight of an atomizing aid, 1.5 parts by weight of glycerol, and 8 parts by weight of deionized water.

[0071] The bactericidal component includes 2 mM potassium sorbate and 3 mM calcium propionate.

[0072] The enhancer includes 2 mM polyethylene glycol 6000 and 4 mM EDTA-2Na.

[0073] The pH adjuster includes 1 mM disodium hydrogen phosphate and 0.05% anhydrous citric acid.

[0074] The atomization aid is propylene glycol.

[0075] The preparation method of the sterilization-based food-grade composite biological agent formula is as follows: the sterilization component and deionized water are mixed evenly, stirred to dissolve, propylene glycol, a synergist and a pH regulator are added, the pH is adjusted to 8, and finally an atomization aid is added and mixed evenly to obtain the sterilization-based food-grade composite biological agent formula.

[0076] Performance Testing

[0077] Test subject: fresh egg surface (simulating contamination with Escherichia coli ATCC 25922 and Salmonella ATCC14028); the food-grade composite biological agent formulation based on atomization sterilization prepared in Examples 1-6 and Comparative Examples 1-3 was prepared at a concentration of 50 ppm and sterilized for 10 seconds for a sterilization test.

[0078] Table 1: Bactericidal test.

[0079] project E. coli killing rate (%) Salmonella killing rate (%) Example 1 99.9 99.7 Example 2 99.9 99.9 Example 3 99.8 99.9 Example 4 99.9 99.8 Example 5 99.8 99.9 Example 6 99.9 99.9 Comparative Example 1 95.2 93.4 Comparative Example 2 96.7 93.6 Comparative Example 3 90.1 91.2 As can be seen from Table 1, Examples 1-6 of the present invention have better sterilization effects than Comparative Examples 1-3.

[0080] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A food-grade compound biological preparation formula based on sterilization, characterized in that: The invention comprises the following components by weight: 60-80 parts by weight of a bactericidal component, 2-5 parts by weight of a synergist, 1-4 parts by weight of a pH regulator, 2-6 parts by weight of an atomizing auxiliary agent, 1-2 parts by weight of glycerol, and 5-10 parts by weight of deionized water.

2. The food-grade compound biological preparation formulation based on sterilization according to claim 1, characterized in that: The bactericidal components include 5000-50000 U lysozyme, 15-50 U lysostaphin, 0.5-5 mM potassium sorbate and 1-5 mM calcium propionate.

3. The food-grade compound biological preparation formulation based on sterilization according to claim 1, characterized in that: The enhancer includes 0.5-4 mM polyethylene glycol 6000 and 2-8 mM EDTA-2Na.

4. The food-grade compound biological preparation formulation based on sterilization according to claim 1, characterized in that: The pH adjuster includes 1-5 mM disodium hydrogen phosphate and 0.05-0.5% anhydrous citric acid.

5. The food-grade compound biological preparation formulation based on sterilization according to claim 1, characterized in that: The atomization aid is propylene glycol.

6. A method for preparing a food-grade compound biological agent formulation based on sterilization according to claims 1-5, characterized in that: The preparation method of the sterilization-based food-grade composite biological agent formula comprises the following steps: uniformly mixing the sterilization component and deionized water, stirring to dissolve, continuously adding glycerol, a synergist, and a pH regulator, adjusting the pH, and finally adding an atomization aid, mixing uniformly, to obtain the sterilization-based food-grade composite biological agent formula.

7. The method for preparing a food-grade composite biological agent formulation based on sterilization according to claim 6, characterized in that: The pH is adjusted to 7.5-8.

8. A food-grade compound biological formulation based on sterilization according to claims 1-5, characterized in that: The application of the method in microbial control of fresh agricultural products.