Anti-wrinkle skin care product composition and mask

Abstract

The invention discloses an anti-wrinkle skin care product composition and a facial mask.The anti-wrinkle skin care product composition comprises an active phase and a basic phase, and the active phase comprises I-type collagen peptide, a ginseng extract, a grape seed extract, an aloe extract and a turmeric extract; the base phase comprises a humectant. According to the technical scheme, the problem that skin collagen is difficult to supplement in a transdermal way is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, in particular to an anti-wrinkle skin care product composition and a facial mask. Background Art

[0002] Collagen and elastic fibers in the skin form a resilient scaffold, providing strong support for the dermis. Collagen, with its triple helical structure, is known as the skin's "deep reservoir," locking in moisture. However, with aging, collagen in the skin gradually denatures and disappears, causing the skin to lose its radiance and moisture, becoming dull and wrinkled.

[0003] For example, if you supplement collagen by eating foods containing collagen, since the collagen content in foods is low and the fat content is high, excessive consumption will lead to increased blood lipids and obesity, and will not increase skin collagen; if you try to supplement skin collagen directly by contacting the skin with external applications, however, because collagen is a large molecule and is blocked by the stratum corneum, collagen cannot enter the dermis, nor can it increase the collagen in the skin to play an anti-wrinkle role. Summary of the Invention

[0004] The main purpose of the present invention is to provide an anti-wrinkle skin care composition and a facial mask to solve the problem that it is difficult to replenish skin collagen through the transdermal route.

[0005] To achieve the above objectives, the present invention provides an anti-wrinkle skin care composition, which comprises the following components:

[0006] Active phase: The active phase includes type I collagen peptide, ginseng extract, grape seed extract, aloe vera extract and turmeric extract;

[0007] Base Phase: The base phase includes moisturizers.

[0008] In one embodiment, calculated by mass percentage, the type I collagen peptide comprises 0.1-10%, ginseng extract comprises 0.3-15%, grape seed extract comprises 1-8%, aloe vera extract comprises 1-5%, and turmeric extract comprises 0.01-0.5%.

[0009] In one embodiment, calculated by mass percentage, the type I collagen peptide comprises 0.1-8%, ginseng extract comprises 0.3-10%, grape seed extract comprises 3-8%, aloe vera extract comprises 1-3%, and turmeric extract comprises 0.01-0.3%.

[0010] In one embodiment, the mass ratio of the type I collagen peptide, ginseng extract, grape seed extract, aloe extract and turmeric extract is (0.1-5): (0.5-10): (3-5): (1-3): (0.01-0.2).

[0011] In one embodiment, the type I collagen peptide is a nanomicelle; and / or the ginseng extract includes Rg3, Rg5, Rk1, Rh4 and Rh1.

[0012] In one embodiment, calculated by mass percentage, in 1% of the ginseng extract, Rg3, Rg5, Rk1, Rh4 and Rh1 are respectively greater than 100 ppm, and the sum is greater than 1000 ppm; and / or, the aloe vera extract is one or more of Aloe barbadensis, Aloe cape or Aloe vera leaf extract.

[0013] In one embodiment, the moisturizing agent includes one or more of butylene glycol, propylene glycol, 1,3-propanediol, 1,2-pentanediol, glycerin, carbomer, sodium hyaluronate, and triethanolamine.

[0014] In one embodiment, the base phase further comprises a preservative, and the preservative comprises one or more of p-hydroxyacetophenone, capryloylglycine, and capryloylhydroxamic acid.

[0015] In one embodiment, the anti-wrinkle skin care composition further comprises DNA zinc, caprylic / capric triglyceride, fragrance and deionized water.

[0016] The present invention also provides a facial mask, wherein the essence of the facial mask comprises an anti-wrinkle skin care composition, and the anti-wrinkle skin care composition comprises the following components:

[0017] Active phase: The active phase includes type I collagen peptide, ginseng extract, grape seed extract, aloe vera extract and turmeric extract;

[0018] Base Phase: The base phase includes moisturizers.

[0019] The technical solution of the present invention adopts type I collagen peptide, ginseng extract, grape seed extract, aloe extract and turmeric extract to synergistically enhance the effect, inhibit the activity of collagenase, and have a high absorption and utilization rate of type I collagen, thereby promoting the synthesis of type I collagen and type III collagen by fibroblasts in the skin, thereby increasing the collagen in the skin, providing strong support for the skin, improving skin elasticity, effectively removing facial fine lines, and having an anti-wrinkle effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0021] Figure 1 The average change rate of the skin elasticity index R2 samples in the skin elasticity test, where *** indicates p < 0.001.

[0022] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0024] It should be noted that if "and / or" appears throughout the text, it means including three parallel solutions. For example, "A and / or B" includes solution A, solution B, or solutions that meet both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that a person of ordinary skill in the art can implement them. If the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0025] As used herein, "comprises," "includes," "contains," "has," "has" or other variations are intended to encompass non-closed inclusions, and no distinction is made between these terms. The term "comprising" means that other steps and ingredients that do not affect the end result may be added. The term "comprising" also includes the terms "mainly consisting of..." and "essentially consisting of..." The compositions and methods / processes of the present invention comprise, consist of, and consist essentially of the essential elements and limitations described herein, as well as any additional or optional ingredients, components, steps, or limitations described herein. All numerical values ​​or expressions used in the specification and claims relating to component amounts, process conditions, etc. should be understood to be modified by "about" in all cases. All ranges relating to the same component or property include endpoints, which can be independently combined. Since these ranges are continuous, they include every value between the minimum and maximum values. It should also be understood that any numerical range cited in this application is intended to include all subranges within that range. As used herein, "parts by weight," "parts by weight," "parts by mass," or "parts by mass" are used interchangeably, and the parts by weight may be any fixed weight expressed in milligrams, grams, or kilograms (e.g., 1 mg, 1 g, 2 g, 5 g, or 1 kg, etc.). For example, a composition consisting of 1 part by weight of component a and 9 parts by weight of component b may be a composition consisting of 1 gram of component a + 9 grams of component b, or 10 grams of component a + 90 grams of component b, etc.

[0026] The present invention provides an anti-wrinkle skin care composition.

[0027] In an embodiment of the present invention, the anti-wrinkle skin care composition comprises the following components:

[0028] Active phase: The active phase includes type I collagen peptide, ginseng extract, grape seed extract, aloe vera extract and turmeric extract;

[0029] Base Phase: The base phase includes moisturizers.

[0030] It is understood that the active phase is the active substance in the anti-wrinkle skincare composition, which plays a primary role in enhancing collagen absorption and synthesis. In addition to the active phase, anti-wrinkle skincare compositions also include a base phase, which can serve auxiliary functions such as carrying the active phase, enhancing the anti-wrinkle effect of the anti-wrinkle skincare composition, and repairing the skin.

[0031] Specifically, type I collagen peptides are used to provide collagen raw materials to the skin, helping to replenish type I collagen in the skin, thereby increasing type I collagen in the dermis, providing strong structural support for the skin, and firming and anti-wrinkle skin. The type I collagen peptides can be animal-derived, such as fish type I collagen peptides or soy type I collagen peptides, which have high absorption rates; or they can be synthesized using genetic engineering technology, which has high biocompatibility and good safety.

[0032] In order to improve the absorption of type I collagen peptide and reduce the risk of allergies, in one embodiment, the type I collagen peptide is in the form of nano-micelles, so that the type I collagen peptide can be quickly absorbed through the skin to reach the dermis, and the transdermal absorption rate is high. In addition, it can promote collagen synthesis and repair damaged collagen, thereby achieving an anti-wrinkle effect. Furthermore, in one embodiment, the type I collagen peptide adopts Taishu of Future Polypeptide Biotechnology (Yantai) Co., Ltd. Type 1 collagen peptides.

[0033] Specifically, ginseng extract not only regulates signaling pathways, inhibits matrix metalloproteinase (MMP) activity, and reduces collagen degradation, but also promotes fibroblast proliferation and migration, enhances fibroblast activity, and promotes fibroblast synthesis of type I and type III collagen, thereby increasing collagen content in the skin, playing an anti-wrinkle role and reducing wrinkles. Furthermore, ginseng extract can increase the absorption rate of type I collagen peptides, further promoting the synthesis and repair of type I collagen in the dermis.

[0034] In one embodiment, the ginseng extract comprises Rg3, Rg5, Rk1, Rh4 and Rh1. Furthermore, in one embodiment, in 1% of the ginseng extract, the content of Rg3, Rg5, Rk1, Rh4 and Rh1 is greater than 100 ppm respectively, and the total content is greater than 1000 ppm.

[0035] The preparation method of the ginseng extract can be: after crushing ginseng, adding a 50% ethanol solution with a mass 20 times that of the ginseng, ultrasonically extracting for 2 hours (power 480W), filtering to obtain a filtrate and a filter residue; adding water with a mass 10 times that of the filter residue to the filter residue, ultrasonically extracting for another 2.5 hours (power 480W), filtering to obtain a filtrate and a filter residue; and combining the two filtrates and concentrating to one-fifth of the original volume to obtain the ginseng extract.

[0036] Specifically, grape seed extract can stimulate fibroblasts to synthesize collagen. It contains peptide compounds such as proanthocyanidins, which have strong antioxidant properties, can scavenge free radicals, inhibit the activity of matrix metalloproteinases, and reduce collagen degradation. In one embodiment, the proanthocyanidin content in grape seed extract is greater than 85%.

[0037] Specifically, turmeric extract can promote collagen synthesis and possesses strong antioxidant properties, neutralizing free radicals and reducing skin damage, further tightening the skin. Additionally, turmeric extract has anti-inflammatory, soothing, and whitening effects. In one embodiment, the turmeric extract is produced by Shaanxi Yunqi Biotechnology Co., Ltd.

[0038] Specifically, aloe vera extract has antioxidant and anti-inflammatory properties, reducing the rate of collagen degradation. It can also promote the proliferation of fibroblasts, thereby enhancing the synthesis of type I and type III collagen. Furthermore, aloe vera extract enhances the transport capacity of type I collagen peptides, further promoting their absorption, increasing collagen content in the skin and playing an anti-wrinkle role. Aloe vera also has repairing and soothing effects, promoting skin metabolism.

[0039] Since the turmeric extract contains curcumin, in one embodiment, the mass ratio of the turmeric extract to the aloe vera extract is 1:(25-60), which can reduce skin pigmentation and skin yellowing.

[0040] In one embodiment, the aloe extract is one or more of Aloe barbadensis, Aloe vera, or Aloe vera leaf extract. The aloe extract can be purchased. In one embodiment, the aloe extract (CAS No. 85507-69-3) from Changsha Shanghe Biotechnology is used.

[0041] The technical solution of the present invention adopts type I collagen peptide as an anti-wrinkle active substance, providing type I collagen raw material, which is beneficial to the synthesis of type I collagen in the skin; ginseng extract is a wrinkle-removing active substance, which not only promotes the synthesis of collagen (type I and type III), but also improves the absorption rate of type I collagen peptide; aloe vera extract has moisturizing, repairing and soothing effects, improves skin elasticity and reduces wrinkles, can promote skin metabolism and reduce pigmentation; grape seed extract and turmeric extract have strong antioxidant effects, which not only inhibit the activity of matrix metalloproteinases and reduce the degradation of skin original collagen, but also protect type I collagen peptide.

[0042] Therefore, the present application uses type I collagen peptides, ginseng extract, grape seed extract, aloe extract and turmeric extract to achieve multiple synergistic effects, which not only increases the absorption and utilization of type I collagen, but also promotes the synthesis of type I collagen and type III collagen by fibroblasts in the skin, thereby supplementing type I collagen, which is the most and most important collagen in the skin, and at the same time increases the synthesis of type III collagen, thereby reducing wrinkles, increasing skin elasticity, and achieving anti-wrinkle and firming effects.

[0043] In one embodiment, calculated by mass percentage, the type I collagen peptide comprises 0.1-10%, ginseng extract comprises 0.3-15%, grape seed extract comprises 1-8%, aloe vera extract comprises 1-5%, and turmeric extract comprises 0.01-0.5%.

[0044] Furthermore, in one embodiment, the type I collagen peptide is 0.1-8%, ginseng extract is 0.3-10%, grape seed extract is 3-8%, aloe vera extract is 1-3% and turmeric extract is 0.01-0.3%.

[0045] Furthermore, in one embodiment, the mass ratio of the type I collagen peptide, ginseng extract, grape seed extract, aloe extract and turmeric extract is (0.1-5):(0.5-10):(3-5):(1-3):(0.01-0.2).

[0046] In one embodiment, the moisturizing agent includes one or more of butylene glycol, propylene glycol, 1,3-propanediol, 1,2-pentanediol, glycerin, carbomer, sodium hyaluronate, and triethanolamine. The moisturizing agent increases the moisture content of the skin, thereby improving skin elasticity and reducing wrinkles.

[0047] In one embodiment, the base phase further comprises a preservative to reduce bacterial growth, and the preservative comprises one or more of p-hydroxyacetophenone, capryloylglycine, and capryloylhydroxamic acid.

[0048] In one embodiment, the anti-wrinkle skin care composition further comprises DNA zinc, caprylic / capric triglyceride, fragrance and deionized water.

[0049] In one embodiment, the anti-wrinkle skin care composition includes, by mass percentage, 0.1-5% of type I collagen peptide, 0.5-10% of ginseng extract, 3-5% of grape seed extract, 1-3% of aloe extract, 0.01-0.2% of turmeric extract, 1-3% of butylene glycol, 1-5% of propylene glycol, 1-3% of glycerin, 0.05-1% of carbomer, 0.01-0.2% of sodium hyaluronate, 0.1-1% of parahydroxyacetophenone, 0.05-5% of capryloylglycine, 0.05-1% of capryloylhydroxamic acid, 0.01-0.1% of DHA zinc, 0.1-1% of caprylic / capric triglyceride, and 0.01-1% of fragrance, and is supplemented with deionized water.

[0050] The present invention also provides a facial mask, the essence of which includes the above-mentioned anti-wrinkle skin care composition. Please refer to the above-mentioned embodiments for the anti-wrinkle skin care composition. Since this facial mask adopts all the technical solutions of all the above-mentioned embodiments, it has at least all the beneficial effects brought about by the technical solutions of the above-mentioned embodiments, which will not be described one by one here.

[0051] It is understandable that the facial mask may further include a sheet-like carrier, through which the essence is applied to the face of a person, and the sheet-like carrier may be made of cotton, plant fiber or synthetic fiber.

[0052] The present invention also provides a method for preparing a wrinkle skin care product composition, comprising the following steps:

[0053] S1. Stir butanediol, propylene glycol and deionized water and heat to 80°C;

[0054] S2. Add carbomer, sodium hyaluronate, and p-hydroxyacetophenone to a homogenizer and homogenize at 80°C at a speed of 2000 rpm for 20 minutes to mix evenly.

[0055] S3. Lower the temperature to below 45°C, add type I collagen peptide, ginseng extract, grape seed extract, aloe extract, turmeric extract, glycerin, capryloyl glycine, capryloyl hydroxamic acid, DHA zinc, caprylic / capric triglyceride, and essence, and stir at a stirring rate of 55 r / min for 1 hour. Stir until evenly mixed to prepare a wrinkle skin care product composition.

[0056] The embodiments of the present invention will be described in detail below with reference to specific examples, but those skilled in the art will appreciate that the following examples are intended only to illustrate the present invention and should not be construed as limiting the scope of the present invention. Where specific conditions are not specified in the examples, the methods were performed according to conventional conditions or the conditions recommended by the manufacturer. Where the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0057] The active phase of the following examples and comparative examples uses the following materials: Type I collagen peptide was purchased from Taishu of Future Peptide Biotechnology (Yantai) Co., Ltd. Type 1 collagen peptide and ginseng extract were obtained according to the above preparation method, grape seed extract was purchased from Xi'an Xinlu Biotechnology Co., Ltd. (CAS No. 84929-27-1), aloe vera extract was purchased from Changsha Shanghe Biotechnology Co., Ltd. (CAS No. 85507-69-3), and turmeric extract was purchased from Shaanxi Yunqi Biotechnology Co., Ltd. (10:1).

[0058] Example 1

[0059] Disclosed are an anti-wrinkle skin care composition and an essence facial mask containing the anti-wrinkle skin care composition. Calculated by mass percentage, the anti-wrinkle skin care composition comprises: 0.1% type I collagen peptide nanomicelles, 2% ginseng extract, 3% grape seed extract, 2% aloe extract, 0.05% turmeric extract, 3% butylene glycol, 5% propylene glycol, 3% glycerol, 0.3% carbomer, 0.05% sodium hyaluronate, 0.2% p-hydroxyacetophenone, 0.5% capryloylglycine, 0.3% capryloylhydroxamic acid, 0.01% DHA zinc, 0.3% caprylic / capric triglyceride, 0.01% essence, and the remainder being made up of deionized water.

[0060] The anti-wrinkle skin care composition is prepared according to the above-mentioned method for preparing an anti-wrinkle skin care composition.

[0061] Examples 2 to 10 respectively provide an anti-wrinkle skin care composition and a facial mask containing the anti-wrinkle skin care composition. The components and mass percentages of the anti-wrinkle skin care composition are shown in Table 1 and are not described in detail here.

[0062] Table 1 Components and contents of the anti-wrinkle skin care product composition

[0063]

[0064]

[0065] Similarly, Comparative Examples 1 to Example 6 respectively provide an anti-wrinkle skin care composition and a facial mask containing the anti-wrinkle skin care composition, prepared using the above-mentioned method for preparing an anti-wrinkle skin care composition. The components and mass percentages of the anti-wrinkle skin care composition are shown in Table 2. At the same time, a popular anti-wrinkle patch-type facial mask was purchased online (JD.com) and offline (Walmart) as Comparative Examples 7 and 8, respectively.

[0066] Table 2 Components and contents of the anti-wrinkle skin care product composition of the comparative example

[0067]

[0068] 1. Type I collagen content test

[0069] Medium 106S (manufactured by Kurashiki Boseki) was used, and 100 μL of 10% weight ratio LSGS (low serum growth supplement, manufactured by Kurashiki Boseki) was added to each well to a concentration of 1.0×10 4 Human dermal fibroblasts at a concentration of 100 cells / mL were seeded into 96-well plates at a volume of 100 μL per well and cultured at 37° C., 5% CO 2 and humidified conditions for 3 days.

[0070] The culture medium was replaced with DMEM (Dulbecco's Modified Eagle's Medium, manufactured by Sigma), and the anti-wrinkle skincare compositions or facial mask essences of the above examples and comparative examples were diluted 10-fold and added for incubation. Deionized water was used as a negative control. After 7 days of cell culture, the culture fluid was collected and the type I collagen content in each culture fluid was quantitatively determined using an enzyme-linked immunosorbent assay (Human Pro-collagen type I c-peptide EIA Kit, 97044ES96, manufactured by Yisheng Biotechnology (Shanghai) Co., Ltd.).

[0071] The actual type I collagen content in the examples and comparative examples = the type I collagen content in the culture medium after 7 days of culture - the type I collagen content in the culture medium before 7 days of culture. The type I collagen content in the culture medium of each example and comparative example was calculated based on the type I collagen content of the negative control (100%). The results are shown in Table 3.

[0072] Table 3 Results of actual type I collagen content of each sample

[0073] sample Type I collagen content (%) Example 1 210.3 Example 2 203.2 Example 3 222.8 Example 4 243.2 Example 5 269.6 Example 6 283.9 Example 7 291.7 Example 8 299.2 Example 9 315.1 Example 10 324.5 Comparative Example 1 113.4 Comparative Example 2 122.8 Comparative Example 3 125.2 Comparative Example 4 124.4 Comparative Example 5 128.7 Comparative Example 6 103.2 Comparative Example 7 134.0 Comparative Example 8 142.8

[0074] The test used the type I collagen content of the negative control as a benchmark, subtracting the type I collagen peptide or fish collagen in the examples and comparative examples, which may affect the test, to obtain the type I collagen content of the culture medium of each example and comparative example. As can be seen from the table above, the type I collagen content in the culture medium of Examples 1 to 10 was greater than 200%, indicating that the anti-wrinkle skin care composition of the present application can promote the formation of type I collagen by fibroblasts and enhance the absorption and synthesis of type I collagen.

[0075] The type I collagen content in the culture medium of Comparative Examples 1 to 6 was less than 130%, indicating that the anti-wrinkle skin care composition had limited ability to synthesize type I collagen. The type I collagen content in the culture medium of Comparative Examples 7 and 8 was 134.0% and 142.8%, respectively, indicating that a certain amount of type I collagen could be synthesized.

[0076] 2. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test

[0077] Human dermal fibroblasts were seeded into DMEM medium containing 10% fetal bovine serum (PBS) and diluted to 5×10 4 cells / mL. 1% of the anti-wrinkle skincare composition of the example and comparative example was added to each well and incubated at 37°C, 5% CO2 for 48 hours. The culture medium was removed, and each well was washed twice with 200 μL of phosphate buffered saline. 20 μL of MTT solution (Yuan Ye Biotechnology Co., Ltd.) was added to each well of DMEM medium and incubated for 2 hours.

[0078] The culture medium was removed, and 150 μL of DMSO (Dimethyl Sulfoxide) solution was added to each well. The wells were gently shaken to dissolve the formazan formed in the cells. The absorbance at 550 nm was measured using a microplate reader with DMSO solution as the control (absorbance 100%). The results are shown in Table 4.

[0079] Table 4 Cell proliferation results of each sample

[0080] sample Cell proliferation (%) Example 1 136.2 Example 2 133.4 Example 3 134.1 Example 4 135.6 Example 5 136.5 Example 6 137.0 Example 7 137.3 Example 8 137.9 Example 9 138.2 Example 10 138.9 Comparative Example 1 113.2 Comparative Example 2 105.3 Comparative Example 3 113.6 Comparative Example 4 109.7 Comparative Example 5 112.9 Comparative Example 6 114.1 Comparative Example 7 122.3 Comparative Example 8 124.8

[0081] As can be seen from Table 4, the proliferation rates of fibroblasts in Examples 1 to 10 are all greater than 130%, indicating that the anti-wrinkle skin care composition of the present application can promote the proliferation of fibroblasts, thereby increasing the synthesis of type I collagen and type III collagen, reducing the formation of wrinkles, and playing an anti-wrinkle role.

[0082] The proliferation rates of Comparative Examples 7 and 8 were 122.3% and 124.8%, respectively, showing some effect on fibroblast proliferation, but lower than the fibroblast proliferation effect of the anti-wrinkle skincare composition of the present application. The fibroblast proliferation rates of Comparative Examples 1 to 6 ranged from 105.3% to 114.1%, indicating that these anti-wrinkle skincare compositions promoted fibroblast proliferation moderately.

[0083] 3. Elastase inhibition test

[0084] Prepare enzyme solution: Dissolve elastase in PBS and dilute to 2.5 U / mL solution.

[0085] Preparation of substrate: The substrate (N-succinyl-alanine-alanine-alanine-p-nitroaniline, produced by Sigma) was dissolved in 10% DMSO (DMSO:PBS=1:9) to prepare a 10 mmol / L solution, which was then diluted to 200 μmol / L.

[0086] Preparation of sample solution: The anti-wrinkle skin care compositions of the examples and comparative examples were diluted 50 times with deionized water.

[0087] 135 μL of the prepared enzyme solution and 15 μL of the sample solution were added to a centrifuge tube. After incubation at 37°C for 5 minutes, 150 μL of the substrate was added. At the same time, a 100% enzyme activity control group (an equal volume of DMSO was used instead of the sample), a blank substrate group (an equal volume of 10% DMSO was used instead of the substrate), and a blank control group were set up. After incubation at 37°C for 60 minutes, the tube was centrifuged (10,000 r / min, 5 minutes) and 200 μL of the supernatant was added to a 96-well enzyme-labeled well. The absorbance was read at 405 nm. The inhibition rate of elastase was calculated according to the following formula. The results are shown in Table 5.

[0088] The inhibition rate formula of elastase is:

[0089] Inhibition rate (%) = 100 × [1-(A 样品 -A 空白底物 ) / A 100％酶活 ].

[0090] Table 5 Elastase inhibition rate results of each sample

[0091]

[0092]

[0093] As shown in Table 5, the elastase inhibition rates for Examples 1 to 10 ranged from 60.78% to 69.33%, demonstrating that the anti-wrinkle skincare compositions of the present application exhibit a high inhibitory effect on elastase activity, thereby reducing collagen degradation and achieving an anti-wrinkle effect. Comparative Examples 7 and 8 exhibited moderate elastase inhibition, with elastase inhibition rates of 26.89% and 22.45%, respectively. Comparative Examples 1 to 6 exhibited relatively weak elastase inhibition, with elastase inhibition rates ranging from 8.47% to 15.89%.

[0094] 4. Skin wrinkle change test

[0095] Fifty-four volunteers, 12 of whom were male, aged 25 to 50, were randomly assigned to a group of three to use one sample (Example or Comparative Example). After cleansing their face, they applied a facial mask containing the anti-wrinkle skincare composition (a synthetic fiber non-woven fabric was used for all other products except the commercially available one) to their face for 15-20 minutes, once daily for 28 consecutive days. During the trial, the volunteers were not allowed to use other anti-wrinkle and anti-aging skincare products and did not change their dietary habits.

[0096] Volunteers' facial skin was tested using a VISIA full-face analyzer, once every seven days (three replicates each time, with the average taken). For each test, the entire face was cleansed with a foam cleanser and allowed to rest for 20 minutes at a constant relative humidity (40-60%) and temperature (22-24°C) before evaluation. The volunteers' test results were recorded, with the number of wrinkles or wrinkle depth before the test considered 100%. The average value for each group is shown in Table 6.

[0097] Table 6 Wrinkle number and wrinkle depth results of each sample

[0098]

[0099]

[0100] As can be seen from Table 6, after 28 days, the number of wrinkles in Examples 1 to 10 was 73.9% to 80.4%, and the wrinkle depth was 75.8% to 81.5%, respectively. That is, the number of wrinkles decreased by 19.6% to 26.1%, and the wrinkle depth decreased by 18.5% to 24.2%, indicating that the anti-wrinkle skin care composition of the present application can reduce the number and depth of wrinkles and achieve an anti-wrinkle effect.

[0101] In contrast, the wrinkle count and wrinkle depth of Comparative Examples 1 to 6 after 28 days were 90.1% to 92.7% and 91.4% to 95.5%, respectively, indicating no significant changes in wrinkles. This was primarily due to the poor anti-wrinkle effect of the anti-wrinkle skin care product compositions, coupled with the fact that anti-wrinkle and anti-aging skin care products were used during the test period.

[0102] 5. Skin elasticity test

[0103] The test conditions were an MPA580 skin tester. The zygomatic area was selected for testing. The inhalation time was 2 seconds, and the relaxation time was 2 seconds. Other conditions were the same as those for the "Skin Wrinkle Change Test." The skin's rebound value (Ua) without negative pressure and the maximum stretch value (Uf) with negative pressure were recorded. The skin elasticity index R2 (overall skin elasticity) was calculated. The results are shown in Table 7. The skin elasticity index R2 is the ratio of Ua to Uf, and the average rate of change is the difference between the skin elasticity index R2 on day 28 and the skin elasticity index R2 on day 0, divided by the skin elasticity index R2 on day 0.

[0104] Table 7 Skin elasticity index R2 results of each sample

[0105]

[0106]

[0107] The closer the skin elasticity index R2 value is to 1, the better the skin elasticity.

[0108] As can be seen from Table 7, after 28 days, the skin elasticity index R2 values ​​of Examples 1 to 10 ranged from 0.767 to 0.830, with an average rate of change greater than 40%, indicating that the anti-wrinkle skin care composition of the present application can improve skin elasticity, significantly replenish the collagen content in the skin, and have a good anti-wrinkle effect.

[0109] The skin elasticity index R2 values ​​of commercially available products Comparative Examples 7 and 8 were 0.697 and 0.722 after 28 days, with average change rates of 0.291 and 0.267; the average change rates of Comparative Examples 1 to 6 after 28 days were between 0.111 and 0.150, indicating that the skin elasticity improvement effect of the comparative examples was average.

[0110] The average rate of change data of the skin elasticity index R2 of the embodiment and the comparative example are processed as follows: Figure 1 The p-values ​​of the average change rates of Examples 1 to 10 and the average change rates of Comparative Examples 1 to 6, and the commercially available products Comparative Example 6 and Comparative Example 8 are all <0.001, indicating that there are extremely significant differences between the example group and Comparative Examples 1-6, and Comparative Examples 7-8. The anti-wrinkle skin care compositions of Examples 1-10 of the present application can improve skin elasticity, and the improvement effect is better than that of commercially available products.

[0111] In summary, the anti-wrinkle skin care composition and facial mask of the present application have a high absorption and utilization rate of type I collagen, promote the activation of fibroblasts in the skin, and facilitate the synthesis of type I collagen and type III collagen by fibroblasts, while inhibiting collagenase activity, thereby not only replenishing the collagen content in the skin but also reducing collagen degradation, thereby reducing wrinkles and firming the skin.

[0112] Unless otherwise specified, the raw materials and equipment used in the present invention are commonly used in the art. Unless otherwise specified, the methods used in the present invention are conventional methods in the art. Unless otherwise specified, the meanings of terms in this specification are the same as those generally understood by those skilled in the art. In the event of any conflict, the definitions in this specification shall prevail.

[0113] The foregoing examples are merely illustrative and serve to illustrate some of the features of the method of the present invention. The appended claims are intended to claim the widest possible scope that can be envisioned, and the embodiments presented herein are merely illustrative of selected implementations according to a combination of all possible embodiments. Therefore, it is the applicant's intention that the appended claims are not limited by the selection of examples illustrating the features of the present invention. Some numerical ranges used in the claims also include subranges therein, and variations in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. An anti-wrinkle skin care composition, characterized in that: Includes the following components: Active phase: The active phase includes type I collagen peptide, ginseng extract, grape seed extract, aloe vera extract and turmeric extract; Base Phase: The base phase includes moisturizers.

2. The anti-wrinkle skin care composition according to claim 1, wherein Calculated by mass percentage, the type I collagen peptide comprises 0.1-10%, ginseng extract 0.3-15%, grape seed extract 1-8%, aloe extract 1-5% and turmeric extract 0.01-0.5%.

3. The anti-wrinkle skin care composition according to claim 2, wherein Calculated by mass percentage, the type I collagen peptide comprises 0.1-8%, ginseng extract 0.3-10%, grape seed extract 3-8%, aloe extract 1-3% and turmeric extract 0.01-0.3%.

4. The anti-wrinkle skin care composition according to claim 3, wherein The mass ratio of the type I collagen peptide, ginseng extract, grape seed extract, aloe extract and turmeric extract is (0.1-5): (0.5-10): (3-5): (1-3): (0.01-0.2).

5. The anti-wrinkle skin care composition according to claim 4, wherein The type I collagen peptide is a nanomicelle; and / or the ginseng extract includes Rg3, Rg5, Rk1, Rh4 and Rh1.

6. The anti-wrinkle skin care composition according to claim 5, wherein Calculated by mass percentage, in 1% of the ginseng extract, Rg3, Rg5, Rk1, Rh4 and Rh1 are each greater than 100 ppm, and the sum of the Rg3, Rg5, Rk1, Rh4 and Rh1 is greater than 1000 ppm; and / or The aloe extract is extracted from one or more leaves of aloe vera, aloe vera or aloe vera.

7. The anti-wrinkle skin care composition according to any one of claims 1 to 6, wherein: The moisturizing agent includes one or more of butylene glycol, propylene glycol, 1,3-propylene glycol, 1,2-pentanediol, glycerin, carbomer, sodium hyaluronate, and triethanolamine.

8. The anti-wrinkle skin care composition according to claim 7, wherein The base phase further includes a preservative, which includes one or more of p-hydroxyacetophenone, capryloylglycine, and capryloylhydroxamic acid.

9. The anti-wrinkle skin care composition according to claim 8, wherein The anti-wrinkle skin care composition further comprises DNA zinc, caprylic / capric triglyceride, essence and deionized water.

10. A facial mask, characterized in that: The essence of the facial mask comprises the anti-wrinkle skin care composition according to any one of claims 1 to 9.

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