Bactericidal composition and application thereof

Abstract

The invention relates to a bactericidal composition. The invention also relates to an application of the bactericidal composition in the field of agriculture, forestry or gardening for preventing or preventing phytopathogen from infecting plants. The bactericidal composition comprises an active component A and an active component B, the active component A is a compound represented by formula (I), the active component B is bronopol and kasugamycin, and the mass ratio of the active component A to the active component B is 1: 24-60: 1. The bactericidal composition disclosed by the invention has a very good prevention and treatment effect on bacterial diseases, especially shows a remarkable synergistic effect on the prevention effect on cucumber bacterial angular leaf spot, and can effectively reduce the dosage, reduce the agricultural input cost and delay the drug resistance of phytopathogen.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide compounding, and in particular relates to a bactericidal composition and application thereof. Background Art

[0002] Cucurbitaceae vegetables are among the most widely cultivated major edible vegetables in my country. Bacterial plant diseases pose a serious threat to food security, often characterized by sudden onset, rapid spread, and difficulty in control. Cucumber bacterial angular leaf spot is a bacterial disease caused by Pseudomonas syringae pv. lachrymans. It can occur in cucumbers during both the seedling and mature stages, primarily infecting leaves. Typical symptoms begin with water-soaked, nearly circular, sunken spots that gradually turn slightly yellowish-brown, with leaf dryness. The disease spreads rapidly, and in severe cases, can reduce cucumber yields by 50%, or even completely eliminate the entire crop.

[0003] Currently, chemical control is still the primary means of preventing and controlling bacterial diseases in plants. However, the agents used to prevent and control these diseases are numerous, chaotic, and ineffective. Faced with increasingly severe control pressures, some producers have resorted to inappropriate measures, such as using multiple doses, resulting in problems such as excessive residues. The present invention surprisingly found that combining the compound of formula (I) with bronopol and kasugamycin, at a specific mass ratio, exhibited a strong control effect against bacterial diseases, particularly demonstrating a significant synergistic effect against bacterial angular leaf spot of cucumber. Summary of the Invention

[0004] Based on the above, the present invention aims to provide a fungicide composition and its use. This fungicide composition, formulated with different active ingredients, effectively reduces production costs. This fungicide composition can be used to effectively control bacterial diseases, reducing drug dosage while delaying the development and progression of drug resistance in pathogenic bacteria.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: a bactericidal composition, the bactericidal composition comprising active ingredient A and active ingredient B, the active ingredient A being a compound represented by formula (I), with a chemical structure of: The active ingredient B is any one of bronopol and kasugamycin;

[0006] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 60:1 or any value within the numerical range above;

[0007] When the active ingredient B is bronopol,

[0008] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 32:1;

[0009] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:24, 1:12, 1:4, 1:1, 4:1, 8:1, 21:1, and 32:1;

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 32:1;

[0011] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:12, 1:4, 1:1, 4:1, 8:1, 21:1, and 32:1;

[0012] When the active ingredient B is kasugamycin,

[0013] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 36:1;

[0014] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:28, 1:14, 1:7, 2:1, 8:1, 15:1, 28:1, and 36:1;

[0015] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 28:1;

[0016] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:14, 1:7, 2:1, 8:1, 15:1, and 28:1;

[0017] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt%, or any value within the above numerical range;

[0018] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 2 to 50 wt%, or any value within the above numerical range;

[0019] Furthermore, the fungicidal composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients;

[0020] Furthermore, the auxiliary ingredients include one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a film former, a warning color, a penetrant, and a carrier;

[0021] Furthermore, the wetting agent is selected from a mixture of one or more of sodium dodecylbenzene sulfate, sodium dodecylbenzene sulfonate, soap powder, alkyl sulfate, pull open powder BX, silkworm feces, alkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether and its phosphate or sulfate ester salt, and alkyl polyoxyethylene ether succinate sulfonate;

[0022] Furthermore, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, naphthalene or alkylnaphthalene formaldehyde condensate sulfonates, alkylbenzene calcium sulfonates, alkylphenol polyoxyethylene phosphates, fatty alcohol polyoxyethylene polyoxypropylene ethers, fatty alcohol polyoxypropylene polyoxypropylene ethers, and polymerized alkylaryl sulfonates;

[0023] Furthermore, the emulsifier is selected from a mixture of one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, and fatty acid polyoxyethylene ester;

[0024] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose;

[0025] Furthermore, the disintegrant is selected from a mixture of one or more of aluminum chloride, bentonite, sucrose, modified starch, cellulose, urea, sodium carbonate, sodium bicarbonate, sodium chloride, sodium sulfate, citric acid and tartaric acid;

[0026] Furthermore, the antifreeze agent is selected from a mixture of one or more of alcohols, alcohol ethers, and inorganic salts;

[0027] Furthermore, the defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 A mixture of one or more fatty alcohol compounds or silicone compounds;

[0028] Furthermore, the solvent is selected from a mixture of one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water;

[0029] Furthermore, the preservative is selected from a mixture of one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate;

[0030] Furthermore, the stabilizer is selected from a mixture of one or more of oxalic acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil;

[0031] Furthermore, the warning color is selected from any one or more adjustment colors of blue, green, red, purple, and yellow;

[0032] Furthermore, the film-forming agent is selected from one or more of sodium carboxymethyl starch, sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol, and polyacrylic acid;

[0033] Furthermore, the carrier is selected from a mixture of one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black;

[0034] Furthermore, the fungicide composition can be prepared into any formulation permitted in agriculture, and the formulation is a solid formulation, a liquid formulation or a seed treatment formulation;

[0035] Furthermore, the solid preparation is a wettable powder or a water-dispersible granule; the liquid preparation is a suspension concentrate, an emulsifiable concentrate, an aqueous emulsion, or a microemulsion; and the seed treatment preparation is a seed treatment suspension concentrate.

[0036] A method for controlling or preventing plant pathogens from infecting plants, wherein the fungicidal composition is used to control agricultural, forestry or horticultural plant pathogens;

[0037] Furthermore, the pathogenic bacteria is a bacterial disease caused by Pseudomonas syringae pv. lachrymans.

[0038] Furthermore, the fungicidal composition is applied in an effective amount to the plant pathogenic bacteria and / or its environment, or to plants, plant propagation materials and subsequently grown plant organs, soil or cultivation media, materials or spaces.

[0039] Compared with the prior art, the present invention has the following advantages:

[0040] 1) The fungicidal composition of the present invention is highly safe and suitable for use in all growth stages of crops;

[0041] 2) The bactericidal composition of the present invention is highly effective in killing bacteria and has a significant synergistic effect under a certain mass ratio;

[0042] 3) The unique mechanism of action of the bactericidal composition of the present invention can effectively protect against resistant pathogens. DETAILED DESCRIPTION

[0043] In order to better illustrate the effective control effect of the present invention, the present invention is described in the following specific examples using the above-mentioned examples to make the technical scheme, purpose and advantages of the present invention more clearly understood, but the present invention is not limited to these examples. The technical effect test of the present invention adopts a combination of indoor bioassay and field test.

[0044] Preparation Example

[0045] Preparation Example 1: 35% formula (I) compound·bronopol wettable powder (28:7)

[0046] Formula composition: by weight percentage, 28% of the compound of formula (I), 7% of bronopol, 7.7% of sodium lignin sulfonate, 4% of calcium dodecylbenzenesulfonate, 12% of kaolin, and bentonite makes up the balance.

[0047] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder product of the composition of the present invention.

[0048] Preparation Example 2: 45% compound of formula (I) and bronopol water dispersible granules (25:20)

[0049] Formula composition: calculated by weight percentage, 25% of the compound of formula (I), 20% of bronopol, 8% of lignin sulfonate, 5% of sodium dodecylbenzenesulfonate, 3% of sodium lauryl sulfate, 5% of white carbon black, and kaolin makes up the balance.

[0050] Preparation method: According to the formula ratio of the embodiment, the active ingredient compound of formula (I) and bronopol are added to a carrier, and a surfactant and other functional additives are added thereto, mixed, and then pulverized by air flow, 10-25% of water is added, and then kneaded, granulated, dried, and sieved to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain a water-dispersible granule product.

[0051] Preparation Example 3: 30% Formula (I) compound·Bronnool soluble solution (12:18)

[0052] Formula composition: calculated by weight percentage, 12% compound of formula (I), 18% bronopol, 3.5% alkylphenol polyoxyethylene ether, 0.3% polyvinyl alcohol 1799, 3.8% glycerol, 0.05% defoaming agent SAF, and deionized water to make up the balance;

[0053] Preparation method: Add the active ingredient to a certain amount of deionized water, stir thoroughly until completely dissolved, then add surfactants, thickeners, antifreeze and other additives in sequence, stir evenly and add appropriate amount of defoaming agent to prepare a soluble liquid product.

[0054] Preparation Example 4: 20% Formula (I) compound·Kasugamycin suspension (15:5)

[0055] Formula composition: by weight percentage, 15% compound of formula (I), 5% kasugamycin, 1.5% alkylphenol polyoxyethylene ether, 1% naphthalenesulfonate formaldehyde condensate, 3% alkylphenol polyoxyethylene ether phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% ethylene glycol, 0.2% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0056] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0057] Preparation Example 5: 22% Formula (I) compound·Kasugamycin soluble solution (18:4)

[0058] Formula composition: calculated by weight percentage, 18% compound of formula (I), 4% kasugamycin, 3% alkylphenol polyoxyethylene ether, 0.3% polyvinyl alcohol 1799, 3% glycerol, 0.05% defoaming agent SAF, and deionized water to make up the balance;

[0059] Preparation method: Add the active ingredient to a certain amount of deionized water, stir thoroughly until completely dissolved, then add surfactants, thickeners, antifreeze and other additives in sequence, stir evenly and add appropriate amount of defoaming agent to prepare a soluble liquid product.

[0060] Indoor activity test

[0061] Example 1:

[0062] Test basis: The test refers to NY / T 1156.6-2006 "Guidelines for Indoor Bioassay of Pesticides" Fungicide Part 6: Determination of the Combined Action of Mixtures;

[0063] NY / T 1156.16-2008 "Guidelines for Indoor Bioassay Tests of Pesticides" Fungicides Part 16: Turbidimetric method for test of inhibition of bacterial growth.

[0064] Pathogen: Pseudomonas syringae pv. lachrymans, the pathogen of cucumber bacterial angular spot, was collected from leaves of cucumber infected with bacterial angular spot in Xiazhuang Street, Chengyang District, Qingdao City, Shandong Province. It was isolated by in vitro diseased tissue separation method, identified by back-inoculation according to Koch's postulates, and inoculated onto NA solid culture medium after purification and stored in a refrigerator at 4°C.

[0065] Drug treatment: Add the drug solution to the sterilized and cooled NB medium according to the experimental design concentration. Set up 4 replicates for each treatment, and set up a treatment containing only solvent and surfactant without active ingredients as a blank control. Dilute the bacteria grown on the slant of NA medium with sterile water to 1×10 7 100 μL of bacterial suspension with a concentration of spores / mL was inoculated into each treatment medium, and the culture was placed at 28°C with shaking (120 r / min).

[0066] Experimental investigation: Measure the turbidity of each treatment before starting the culture. When the control treatment reaches the logarithmic growth phase, measure and record the turbidity of each treatment.

[0067] The control effect is calculated according to the following formula

[0068]

[0069] Where:

[0070] P——growth inhibition rate, in %;

[0071] A0——blank control turbidity increase value;

[0072] A1——Increase in turbidity after chemical treatment.

[0073] The EC of each drug was calculated by regression analysis of the logarithmic value of drug concentration and the probability of efficacy using DPS (data processing system) 50 、EC 90 The values ​​and their 95% confidence limits were calculated, and the significance of the differences among the treatments was analyzed.

[0074] The co-toxicity coefficient (CTC) of the mixture was calculated according to the Sun Yunpei method to evaluate the type of combined action.

[0075] CTC ≥ 120 showed a synergistic effect, CTC ≤ 80 showed an antagonistic effect, and CTC between 80 and 120 showed an additive effect.

[0076] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0077]

[0078] Where:

[0079] ATI - measured toxicity index of mixture;

[0080] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0081] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0082] TTI=TI A *P A +TI B *P B

[0083] Where:

[0084] TTI – Theoretical Toxicity Index of Mixtures;

[0085] TI A ——Agent toxicity index;

[0086] P A ——The percentage of agent A in the mixture, in percentage (%);

[0087] TI B ——Toxicity index of agent B;

[0088] P B ——The percentage of agent B in the mixture, in percentage (%).

[0089]

[0090] Where:

[0091] CTC – Co-toxicity coefficient;

[0092] ATI - measured toxicity index of mixture;

[0093] TTI - Theoretical Toxicity Index of Mixture.

[0094] Test results:

[0095] The single agent of the compound of formula (I), bronopol and kasugamycin has a good inhibitory effect on the pathogen of cucumber bacterial angular leaf spot. As shown in Table 1, the compound of formula (I) and bronopol showed a good antibacterial effect on cucumber bacterial angular leaf spot. When the mass ratio of the compound of formula (I) to bronopol is within the range of 1:24 to 60:1, the co-toxicity coefficient is greater than 80, and the combined effect shows an additive or synergistic effect; when the mass ratio of the compound of formula (I) to bronopol is 1:24 to 32:1, the co-toxicity coefficient is greater than 120, showing a synergistic effect; when the mass ratio of the compound of formula (I) to bronopol is 1:12 to 32:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is significant; when the mass ratio of the compound of formula (I) to bronopol is 4:1, the co-toxicity coefficient is the largest, 251.847, and the synergistic effect is most significant.

[0096] Table 1 Results of indoor combined effect test on bacterial angular spot of compound of formula (I) and bronopol

[0097]

[0098]

[0099] As shown in Table 2, the combination of the compound of formula (I) and kasugamycin exhibited a good antibacterial effect against bacterial angular leaf spot of cucumber. When the mass ratio of the compound of formula (I) to kasugamycin was within the range of 1:28 to 36:1, the cotoxicity coefficient was greater than 120, indicating a synergistic effect. When the mass ratio of the compound of formula (I) to kasugamycin was between 1:14 and 36:1, the cotoxicity coefficient was greater than 140, indicating a significant synergistic effect. When the mass ratio of the compound of formula (I) to kasugamycin was 8:1, the cotoxicity coefficient reached a maximum of 241.914, indicating the most significant synergistic effect.

[0100] Table 2 Results of indoor combined effect test on bacterial angular spot of compound of formula (I) and kasugamycin

[0101]

[0102] Field efficacy trials

[0103] Field trials on the efficacy of pesticides against bacterial angular leaf spot of cucumber

[0104] Test crop: cucumber

[0105] Test subject: Bacterial angular leaf spot (Pseudomonas syringae pv. lachrymans)

[0106] Experimental location: Qingzhou arched heat-insulated vegetable greenhouse, Weifang City, Shandong Province.

[0107] Application time: April 24 and May 15, 2023, a total of two applications. The weather was good during the test.

[0108] Experimental Field Conditions: The soil fertility of the experimental plots is moderate, the terrain is flat, the fertility is uniform, and the irrigation conditions are good. Cultivation conditions (soil type, fertilization, tillage, row spacing, etc.) are uniform across all experimental plots and consistent with local agricultural cultivation practices (GAP).

[0109] Disease situation in the experimental field: At the beginning of the experiment, sporadic ulcer spots were visible on the leaves of the plants.

[0110] Experimental treatment: Each treatment was arranged in random blocks, with buffer rows set between adjacent cells, repeated 4 times, with each treatment 20m 2 Specific experimental treatments and dosages are as follows:

[0111] Table 3 Experimental treatment and dosage

[0112] Pharmacy <![CDATA[Active ingredient application rate (a.i g / hm 2 )]]> 30% formula (I) compound·bronopol soluble solution (12:18) 225 22% Formula (I) compound·Kasugamycin soluble solution (18:4) 110 25% bronopol water dispersible granules 150 6% Kasugamycin soluble solution 60 20% Bifemetstrobin water dispersible granules 150 Clear water control /

[0113] Survey method: Random sampling was conducted at 5 points in each plot, and all leaves of 3 plants were surveyed at each point. The leaves were graded according to the percentage of the lesion area to the whole leaf area, and the disease index and control effect were calculated. The data were analyzed for significance using Duncan's new multiple range method.

[0114] The specific grading standards are:

[0115] Level 0: no lesions;

[0116] Level 1: The lesion area accounts for 5% or less of the entire leaf area;

[0117] Level 3: The lesion area accounts for 6% to 10% of the entire leaf area;

[0118] Level 5: The lesion area accounts for 11% to 20% of the entire leaf area;

[0119] Level 7: The lesion area accounts for 21% to 50% of the entire leaf area;

[0120] Level 9: The lesion area accounts for more than 51% of the entire leaf area.

[0121] Survey time and frequency: Survey the disease base before spraying, and survey once 7 days and 14 days after the last spraying.

[0122] The efficacy is calculated according to the following formula:

[0123]

[0124] During the experiment, cucumbers in each treatment plot grew well and no phytotoxicity was observed in any treatment.

[0125] The results of the field efficacy test are shown in the table below:

[0126] The field efficacy test results in the table above indicate that Bifemetstrobin, combined with bronopol and kasugamycin, exhibited excellent control efficacy against cucumber bacterial angular leaf spot. As shown in Table 4, seven days after the last application, the control efficacy of a 30% solution of the compound of formula (I) in a bronopol soluble solution (12:18) and a 22% solution of the compound of formula (I) in a kasugamycin soluble solution (18:4) against cucumber bacterial angular leaf spot was 85.31% and 84.11%, respectively.

[0127] Table 4 Field efficacy test results of each treatment on bacterial angular leaf spot of cucumber (7 days after the last application)

[0128]

[0129] As shown in Table 5, 7 days after the last application, the protective effects of 30% compound of formula (I)·bronopol soluble solution (12:18) and 22% compound of formula (I)·kasugamycin soluble solution (18:4) on bacterial angular leaf spot of cucumber were 86.88% and 87.46%, respectively.

[0130] Table 5 Field efficacy test results of each treatment on bacterial angular leaf spot of cucumber (14 days after the last application)

[0131]

[0132] In summary, through indoor toxicity assays and field efficacy tests, it can be seen that the bifemetstrobin fungicidal composition of the present invention has a good control effect on plant pathogens, is safe for target crops, has significant control effects, is superior to a single agent in delaying the development of drug resistance and prolonging the effectiveness, can effectively reduce costs, and reduce drug residues.

[0133] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition, characterized in that The bactericidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is any one of bronopol and kasugamycin.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:24 to 60:

1.

3. The bactericidal composition according to claim 1, characterized in that The active ingredient B is bronopol, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 32:1, the active ingredient B is kasugamycin, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 36:1; Preferably, the active ingredient B is bronopol, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 32:1; the active ingredient B is kasugamycin, and the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 28:

1.

4. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt%, and the sum of the content of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 80 wt%; Preferably, the total content of the active ingredient A and the active ingredient B in the bactericidal composition is 2 to 50 wt%.

5. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises auxiliary ingredients in addition to active ingredients.

6. The bactericidal composition according to claim 5, characterized in that The auxiliary components include one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a film-forming agent, a warning color, a penetrant and a carrier.

7. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation permitted in agriculture, and the formulation is a solid formulation, a liquid formulation or a seed treatment formulation.

8. A method for controlling or preventing plant pathogens from infecting plants, characterized in that: The fungicidal composition according to claim 1 is used for preventing and controlling pathogenic bacteria in agricultural, forestry or horticultural plants.

9. The method according to claim 8, wherein The pathogenic bacteria is a bacterial disease caused by Pseudomonas syringae pv. lachrymans.

10. The method according to claim 8, wherein The fungicidal composition is applied in an effective amount to the plant pathogenic bacteria and / or its environment, or to plants, plant propagation materials and subsequently grown plant organs, soil or cultivation media, materials or spaces.

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