Traditional Chinese medicine composition for treating cerebral embolism and preparation and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and provides a traditional Chinese medicine composition for treating cerebral embolism as well as a preparation and application thereof, and the traditional Chinese medicine composition comprises the following components: ligusticum wallichii, eupatorium, leech, turtle shell, fried endothelium corneum gigeriae galli, radix cyathulae, cistanche, salvia miltiorrhiza, astragalus membranaceus, immature bitter orange, mangnolia officinalis, dogwood, rhizoma acori graminei and kadsura pepper stem. Ligusticum wallichii and fortune eupatorium herb have the effects of promoting qi circulation, relieving depression, promoting blood circulation and relieving pain, and are monarch drugs; leech, turtle shell, fried endothelium corneum gigeriae galli and radix cyathulae are used as ministerial drugs, are used for stimulating the menstrual flow, activating blood circulation and removing blood stasis, are used for assisting the monarch drugs to play roles and are used for enhancing the curative effect of the monarch drugs; radix salviae miltiorrhizae, radix astragali, fructus aurantii immaturus, cortex magnoliae officinalis, fructus corni and rhizoma acori graminei jointly serve as adjuvant drugs and have the effects of tonifying qi, consolidating the root The whole formula has the effects of tonifying qi, activating blood, clearing and activating the channels and collaterals.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicines, and relates to a traditional Chinese medicine composition for treating cerebral embolism, and a preparation and use thereof. Background Art

[0002] Cerebral embolism is a common clinical cerebrovascular disease. It is caused by emboli entering the cerebral arteries along the blood circulation system, resulting in occlusion of the arterial lumen. The main clinical manifestations are hemiplegia and slurred speech. There are many causes of cerebral embolism, and advanced age, a history of hypertension, and elevated fibrinogen levels can all be triggers. The disease has a rapid onset and progression. If not treated promptly, it can be complicated by cerebral hemorrhage and other complications, threatening the patient's life. At the same time, cerebral embolism is often accompanied by neurological deficits such as neurological deficits, impaired consciousness, and decreased cognitive function, which can lead to reduced ability to perform daily activities. Timely treatment is required after the onset of cerebral embolism to effectively promote the recovery of brain tissue function, improve brain hypoxia and local blood flow, and prevent brain cell death.

[0003] The principles of treating cerebral embolism are to reduce intracranial pressure, unclog blood vessels, lower blood viscosity, control platelet aggregation, and inhibit the formation of related embolic substances in the blood. Currently, oral medications, including vasodilators, antiplatelet agents, and anticoagulants, are the primary treatment for cerebral embolism.

[0004] Existing drugs are generally ineffective in treating cerebral embolism and are prone to cause adverse reactions such as edema and brain herniation, which are not conducive to the prognosis of the disease. It is very necessary to strengthen effective therapeutic intervention for patients with cerebral embolism. Summary of the Invention

[0005] In light of this, the present invention provides a traditional Chinese medicine composition for treating cerebral embolism, its preparation, and use. The traditional Chinese medicine composition of the present invention significantly improves neurological dysfunction associated with cerebral embolism, reduces cerebral infarction area, and has a positive therapeutic effect on cerebral embolism. It also has a low incidence of toxic side effects and adverse reactions, and can be used as an alternative or supplemental treatment for clinical treatment.

[0006] The present invention also provides a method for preparing the traditional Chinese medicine composition for treating cerebral embolism.

[0007] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition for treating cerebral embolism in preparing a medicine for treating cerebral embolism.

[0008] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are as follows: The first aspect of the present invention provides a traditional Chinese medicine composition for treating cerebral embolism, which is prepared from the following raw materials, by weight: 15-25 parts of Chuanxiong, 15-25 parts of Cyperus rotundus, 15-25 parts of Hirudo, 12-18 parts of turtle shell, 15-25 parts of stir-fried chicken gizzard lining, 12-18 parts of Cyathula, 12-18 parts of Cistanche deserticola, 12-18 parts of Salvia miltiorrhiza, 10-15 parts of Astragalus, 10-15 parts of Citrus aurantium, 15-25 parts of Magnolia officinalis, 12-18 parts of Cornus officinalis, 15-20 parts of Acorus calamus and 8-12 parts of Piper mesenteriae.

[0009] As a preferred technical solution of the present invention, it is prepared from the following raw materials, which are as follows by weight: 20 parts of Chuanxiong, 20 parts of Cyperus rotundus, 20 parts of Hirudo, 15 parts of Carapax Trionycis, 20 parts of stir-fried Chicken Gizzard Stone, 15 parts of Cyathula, 15 parts of Cistanche, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 12 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus calamus and 10 parts of Piper mesenteriae.

[0010] As a preferred technical solution of the present invention, it is prepared from the following raw materials, which are as follows by weight: 15 parts of Chuanxiong, 25 parts of Cyperus rotundus, 15 parts of Hirudo, 18 parts of Carapax tricolor, 25 parts of stir-fried chicken gizzard lining, 18 parts of Cyathula capitata, 18 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 15 parts of Astragalus membranaceus, 15 parts of Citrus aurantium, 25 parts of Magnolia officinalis, 12 parts of Cornus officinalis, 20 parts of Acorus calamus and 8 parts of Piper mesenteriae.

[0011] As a preferred technical solution of the present invention, it is prepared from the following raw materials, by weight: 25 parts of Chuanxiong, 15 parts of Cyperus rotundus, 25 parts of Hirudo, 12 parts of Carapax Trionycis, 15 parts of stir-fried Chicken Gizzard Stone, 12 parts of Cyathula, 12 parts of Cistanche, 12 parts of Salvia miltiorrhiza, 10 parts of Astragalus, 10 parts of Citrus aurantium, 15 parts of Magnolia officinalis, 18 parts of Cornus officinalis, 15 parts of Acorus calamus and 12 parts of Piper mesenteriae.

[0012] The second aspect of the present invention provides a method for preparing the above-mentioned traditional Chinese medicine composition for treating cerebral embolism, comprising the following steps: weighing raw materials according to a formula, mixing, adding 7-9 times the weight of the mixture in water to the mixture, soaking for 1.5-2 hours, decocting the obtained soaking liquid to 110-130°C, cooling to 90-110°C and decocting for 0.5-1.5 hours, filtering to obtain a first decoction and medicinal residues; adding 5-7 times the weight of the medicinal residues in water to the medicinal residues, then boiling to 110-130°C, cooling to 90-110°C and decocting for 0.5-1.5 hours, filtering to obtain a second decoction; mixing the first decoction and the second decoction, concentrating, filtering, and vacuum drying to obtain the product.

[0013] As a preferred technical solution of the present invention, the preparation method of the traditional Chinese medicine composition for treating cerebral embolism comprises the following steps: weighing raw materials according to a formula, mixing, adding 7 times the weight of the mixture in water to the mixture, soaking for 2 hours, decocting the obtained soaking liquid to 110° C., cooling to 90° C. and decocting for 1.5 hours, filtering to obtain a first decoction and medicinal residues; adding 7 times the weight of the medicinal residue in water to the medicinal residue, then boiling to 110° C., cooling to 90° C. and decocting for 0.5 hours, filtering to obtain a second decoction; mixing the first decoction and the second decoction, concentrating, filtering, and vacuum drying to obtain the product.

[0014] The third aspect of the present invention provides a Chinese medicine preparation of a Chinese medicine composition for treating cerebral embolism, characterized in that the Chinese medicine preparation is prepared using the above-mentioned Chinese medicine composition for treating cerebral embolism as a raw material.

[0015] Furthermore, the Chinese medicine preparation also includes pharmaceutically acceptable excipients. The dosage form of the Chinese medicine preparation is Chinese medicine decoction, decoction, granules, pills, capsules, tablets, tea, suspension, powder or oral liquid.

[0016] Furthermore, the dosage form of the Chinese medicine preparation is tablets.

[0017] The fourth aspect of the present invention provides use of the traditional Chinese medicine composition for treating cerebral embolism in preparing a drug for preventing or treating cerebral embolism.

[0018] The properties, flavors, meridians and functions of the main, secondary, auxiliary and guiding drugs in the prescription of the present invention are as follows: 1. Main herbs: Chuanxiong, Peilan Chuanxiong: spicy in flavor, warm in nature, enters the liver and gallbladder meridians, promotes qi and relieves depression, dispels wind and dampness, activates blood circulation and relieves pain; mainly treats wind-cold headache and dizziness, flank pain and abdominal pain, cold arthritis and muscle spasms, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncles and ulcers; used for irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to lumps, stabbing pain in the chest and flanks, swelling and pain from falls, headache, and rheumatic pain.

[0019] Perilla: spicy in taste, neutral in nature, enters the spleen, stomach, and lung meridians, has aromatic properties to eliminate dampness, invigorate the spleen and appetite, and relieve heat; used for heatstroke and loss of appetite. Perilla contains a variety of volatile oils, alkaloids, sterols and their esters, organic acids and other ingredients, which have multiple pharmacological effects such as promoting digestion, anti-inflammatory, and anti-pathogenic microorganisms.

[0020] 2. Assistant medicines: leech, turtle shell, stir-fried chicken gizzard lining, and Sichuan cyathula Leech: salty and bitter in taste, neutral in nature, enters the liver meridian, breaks blood, removes blood stasis, and promotes menstruation; used for abdominal masses, blood stasis, amenorrhea, and injuries from falls.

[0021] Turtle shell: salty in taste, slightly cold in nature, enters the liver and kidney meridians, nourishes yin and suppresses yang, softens and disperses lumps; "Shennong's Herbal Classic" says: "It is used to treat hard masses in the heart and abdomen, remove polyps, and hemorrhoids." "Materia Medica Treatise" says: "It is used to treat lumps, abdominal masses, and cold masses."

[0022] Fried Chicken Gizzard: sweet and neutral in nature, enters the spleen, stomach, small intestine and bladder meridians, strengthens the stomach and aids digestion, astringes semen and stops nocturnal emission; mainly used for vomiting, diarrhea, indigestion, infantile malnutrition, enuresis and nocturnal emission.

[0023] Sichuan Achyranthes: sweet, slightly bitter in taste, neutral in nature, enters the liver and kidney meridians, dispels wind and dampness, promotes menstruation and activates blood circulation; used to treat rheumatic pain in the waist and knees, foot atrophy and muscle spasms, hematuria, hematuria, amenorrhea in women, masses, retained placenta, joint pain, foot atrophy and muscle spasms, hematuria and hematuria, and injuries from falls.

[0024] 3. Adjuvants: Salvia miltiorrhiza, Astragalus membranaceus, Citrus aurantium, Magnolia officinalis, Cornus officinalis, Acorus tatarinowii Salvia miltiorrhiza: bitter in nature and slightly cold, enters the heart and liver meridians, removes blood stasis and relieves pain, promotes blood circulation and dredges menstruation, clears the heart and relieves restlessness; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, stabbing pain in the chest and abdomen, pain due to heat rheumatic arthritis, swollen and painful sores, restlessness and insomnia; hepatosplenomegaly, and angina pectoris.

[0025] Astragalus: sweet in taste, slightly warm in nature, enters the lung and spleen meridians, replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, produces body fluid and nourishes blood, relieves stagnation and arthritis, removes toxins and discharges pus, heals sores and promotes tissue regeneration; used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, chronic diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, edema due to qi deficiency, internal heat and thirst, sallow complexion due to blood deficiency, hemiplegia, pain and numbness, carbuncles that are difficult to heal, and long-term ulcers that do not heal.

[0026] Citrus aurantium: bitter, pungent and sour in taste, slightly cold in nature, enters the spleen and stomach meridians, breaks up qi and eliminates accumulation, resolves phlegm and relieves abdominal distension; used for internal stagnation, fullness and pain in the abdomen, heaviness after diarrhea, constipation, phlegm stagnation and qi obstruction, chest pain, chest congestion, and sagging of internal organs.

[0027] Magnolia officinalis: bitter and pungent in taste, warm in nature, enters the spleen meridian, stomach meridian, and large intestine meridian, promotes qi and eliminates stagnation; dries dampness and eliminates fullness; reduces adverse reactions and relieves asthma; mainly treats food stagnation and qi stagnation; abdominal distension and constipation; dampness blocking the middle burner, abdominal distension, vomiting and diarrhea; phlegm congestion and qi stagnation; chest fullness, wheezing and coughing.

[0028] Cornus officinalis: sour, astringent, slightly warm in nature, enters the liver and kidney meridians, nourishes the liver and kidneys, astringes essence and strengthens the body; used for dizziness and tinnitus, soreness of the waist and knees, impotence and spermatorrhea, enuresis and frequent urination, metrorrhagia and leukorrhea, profuse sweating and collapse, internal heat and thirst.

[0029] Acorus calamus: pungent and bitter in taste, warm in nature, enters the heart and stomach meridians, opens the orifices and eliminates phlegm, wakes up the mind and improves intelligence, eliminates dampness and stimulates appetite; used for coma, epilepsy, forgetfulness, insomnia, tinnitus, deafness, abdominal distension without hunger, and dysentery due to lockjaw.

[0030] 4. Adjuvant: Sea Breeze Vine Piper mestoides: It tastes pungent and bitter, is slightly warm in nature, enters the liver meridian, dispels rheumatism, dredges the meridians, and relieves pain; it is mainly used to treat wind-cold-dampness arthritis, limb pain, tendon spasm and difficulty in flexion and extension.

[0031] The secret of compatibility: In the traditional Chinese medicine composition for treating cerebral embolism of the present invention, Chuanxiong and Perilla frutescens are the main herbs, promoting qi and relieving depression, activating blood circulation and relieving pain. Hirudo, turtle shell, stir-fried chicken gizzard lining, and Cyathula officinalis are the assistant herbs, promoting menstruation and activating blood circulation, removing blood stasis and promoting menstruation, and assisting the main herbs in exerting their effects, thereby enhancing the efficacy of the main herbs. Salvia miltiorrhiza, Astragalus membranaceus, Citrus aurantium, Magnolia officinalis, Cornus officinalis, and Acorus gramineus are the adjuvant herbs, tonifying qi and strengthening the body, removing blood stasis and relieving pain. Piper mesenteriae pulsatillae is the guiding herb, promoting menstruation and relieving pain. The entire formula works together to invigorate qi and activate blood circulation, promoting menstruation and activating collaterals.

[0032] The beneficial effects of the Chinese medicine composition for treating cerebral embolism of the present invention are: The traditional Chinese medicine composition for treating cerebral embolism of the present invention can significantly improve the neurological dysfunction of the cerebral embolism model, significantly reduce the area of ​​cerebral infarction, and has a positive therapeutic effect on cerebral embolism.

[0033] Only by adopting the complete formulation of the present invention can the best therapeutic effect be achieved. If some of the components are discarded or the compatibility ratio is changed, the therapeutic effect of the composition will be significantly affected. The components of the present invention synergize with each other.

[0034] The traditional Chinese medicine composition for treating cerebral embolism of the present invention has low toxic side effects and adverse reaction incidence, and can be used as a substitute or supplementary treatment for clinical treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 Comparison of neurological dysfunction scores among rats in each group.

[0036] Figure 2 Comparison of EB content in rats of each group.

[0037] Figure 3 Comparison of brain water content among rats in each group.

[0038] Figure 1-3 Different lowercase letters above the bar graph indicate significant differences among different groups (P < 0.05). DETAILED DESCRIPTION

[0039] To verify the efficacy of the traditional Chinese medicine composition for treating cerebral embolism, the inventors conducted pharmacodynamic studies. It should be noted that the drugs selected for the pharmacodynamic studies were those obtained from representative formulations and preparation methods of the present invention. Due to space limitations, the tests and results related to other formulations and preparation methods encompassed by the present invention are not exhaustively listed here.

[0040] Example 1 A Chinese medicine composition for treating cerebral embolism 20 parts of Chuanxiong, 20 parts of Cyperus rotundus, 20 parts of Hirudo, 15 parts of Carapax Trionycis, 20 parts of stir-fried Chicken Gizzard Stone, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 12 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus gramineus and 10 parts of Piper mesenteriae.

[0041] The raw materials are weighed and mixed according to the formula, and water 7 times the weight of the mixture is added to the mixture, and the mixture is soaked for 2 hours. The obtained soaking liquid is decocted to 130° C., and then cooled to 110° C. and decocted for 1.5 hours, and filtered to obtain a first decoction and a medicinal residue; water 7 times the weight of the medicinal residue is added to the medicinal residue, and then boiled to 110° C., and then cooled to 90° C. and decocted for 0.5 hours, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0042] Example 2 A Chinese medicine composition for treating cerebral embolism 15 parts of Chuanxiong, 25 parts of Cyperus rotundus, 15 parts of Hirudo, 18 parts of Carapax Trionycis, 25 parts of stir-fried Chicken Gizzard Stone, 18 parts of Cyathula, 18 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 15 parts of Astragalus, 15 parts of Citrus aurantium, 25 parts of Magnolia officinalis, 12 parts of Cornus officinalis, 20 parts of Acorus calamus and 8 parts of Piper mesenteriae.

[0043] The raw materials are weighed according to the formula, mixed, and water 9 times the weight of the mixture is added to the mixture, soaked for 0.5, and the obtained soaking liquid is decocted to 130° C., then cooled to 110° C. and decocted for 1.5 hours, and filtered to obtain a first decoction and medicinal residue; water 5 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 130° C., then cooled to 110° C. and decocted for 1.5 hours, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0044] Example 3 A Chinese medicine composition for treating cerebral embolism 25 parts of Chuanxiong, 15 parts of Cyperus rotundus, 25 parts of Hirudo, 12 parts of Carapax Trionycis, 15 parts of stir-fried Chicken Gizzard Stone, 12 parts of Cyathula, 12 parts of Cistanche, 12 parts of Salvia miltiorrhiza, 10 parts of Astragalus, 10 parts of Citrus aurantium, 15 parts of Magnolia officinalis, 18 parts of Cornus officinalis, 15 parts of Acorus gramineus, and 12 parts of Piper mesenteriae.

[0045] The raw materials are weighed and mixed according to the formula, and water 7 times the weight of the mixture is added to the mixture, soaked for 0.5 h, and the obtained soaking liquid is decocted to 130° C., then cooled to 110° C. and decocted for 0.5 h, and filtered to obtain a first decoction and medicinal residue; water 5 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 130° C., then cooled to 110° C. and decocted for 0.5 h, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0046] Example 4 A Chinese medicine composition for treating cerebral embolism 15 parts of Chuanxiong, 20 parts of Cyperus rotundus, 25 parts of Hirudo, 18 parts of Carapax Trionycis, 25 parts of stir-fried Chicken Gizzard Stone, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 15 parts of Astragalus, 12 parts of Citrus aurantium, 15 parts of Magnolia officinalis, 18 parts of Cornus officinalis, 18 parts of Acorus gramineus and 10 parts of Piper mesenteriae.

[0047] The raw materials are weighed and mixed according to the formula, and water 9 times the weight of the mixture is added to the mixture, soaked for 0.5 h, and the obtained soaking liquid is decocted to 110° C., then cooled to 90° C. and decocted for 1.5 h, and filtered to obtain a first decoction and medicinal residue; water 5 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 110° C., then cooled to 90° C. and decocted for 0.5 h, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0048] Example 5 A Chinese medicine composition for treating cerebral embolism 20 parts of Chuanxiong, 15 parts of Cyperus rotundus, 15 parts of Hirudo, 16 parts of Carapax Trionycis, 20 parts of stir-fried Chicken Gizzard Stone, 18 parts of Cyathula, 18 parts of Cistanche deserticola, 12 parts of Salvia miltiorrhiza, 10 parts of Astragalus, 10 parts of Citrus aurantium, 25 parts of Magnolia officinalis, 16 parts of Cornus officinalis, 15 parts of Acorus gramineus, and 12 parts of Piper mesenteriae.

[0049] The raw materials are weighed and mixed according to the formula, and water 9 times the weight of the mixture is added to the mixture, soaked for 0.5 h, and the obtained soaking liquid is decocted to 110° C., then cooled to 90° C. and decocted for 1.5 h, and filtered to obtain a first decoction and medicinal residue; water 5 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 110° C., then cooled to 90° C. and decocted for 1.5 h, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0050] Example 6 A Chinese medicine composition for treating cerebral embolism 25 parts of Chuanxiong, 25 parts of Cyperus rotundus, 20 parts of Hirudo, 12 parts of Carapax Trionycis, 15 parts of stir-fried Chicken Gizzard Stone, 12 parts of Cyathula, 12 parts of Cistanche, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 15 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 12 parts of Cornus officinalis, 20 parts of Acorus calamus and 8 parts of Piper mesenteriae.

[0051] The raw materials are weighed and mixed according to the formula, and water 7 times the weight of the mixture is added to the mixture, and the mixture is soaked for 2 hours. The obtained soaking liquid is decocted to 110° C., and then cooled to 90° C. and decocted for 1.5 hours, and filtered to obtain a first decoction and a medicinal residue; water 7 times the weight of the medicinal residue is added to the medicinal residue, and then boiled to 110° C., and then cooled to 90° C. and decocted for 0.5 hours, and filtered to obtain a second decoction; the first decoction and the second decoction are mixed, concentrated, filtered, and vacuum-dried to obtain.

[0052] Comparative Example 1 20 parts of leech, 15 parts of turtle shell, 20 parts of stir-fried chicken gizzard lining, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 12 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus gramineus and 10 parts of Piper mesenteriae.

[0053] Compared with Example 1, the difference is that Chuanxiong and Peilan are not contained, and other components and dosages remain unchanged.

[0054] The preparation method is as in Example 1.

[0055] Comparative Example 2 20 parts of Chuanxiong, 20 parts of Perilla frutescens, 20 parts of stir-fried chicken gizzard lining, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 12 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus gramineus and 10 parts of Piper mesenteriae.

[0056] Compared with Example 1, the difference is that leech and turtle shell are not contained, and other components and dosages remain unchanged.

[0057] The preparation method is as in Example 1.

[0058] Comparative Example 3 20 parts of Chuanxiong, 20 parts of Cyperus rotundus, 20 parts of Hirudo, 15 parts of Carapax Trionycis, 20 parts of stir-fried Chicken Gizzard Stone, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 12 parts of Immature Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus calamus and 10 parts of Caulis Piperis d'Azurii.

[0059] Compared with Example 1, the difference is that Salvia miltiorrhiza and Astragalus membranaceus are not contained, and the other components and dosages remain unchanged.

[0060] The preparation method is as in Example 1.

[0061] Comparative Example 4 30 parts of Chuanxiong, 10 parts of Cyperus rotundus, 10 parts of Hirudo, 20 parts of Carapax Trionycis, 10 parts of stir-fried Chicken Gizzard Stone, 10 parts of Cyathula, 20 parts of Cistanche deserticola, 10 parts of Salvia miltiorrhiza, 20 parts of Astragalus, 20 parts of Citrus aurantium, 10 parts of Magnolia officinalis, 10 parts of Cornus officinalis, 12 parts of Acorus calamus and 6 parts of Piper mesenteriae.

[0062] Compared with Example 1, the difference is that the dosage of each component is different.

[0063] The preparation method is as in Example 1.

[0064] Study on the effect of the Chinese medicine composition of the present invention on cerebral embolism model rats 1. Experimental Methods Among 54 male Wistar rats, 6 were randomly selected as the sham operation group and the rest were used as the model group.

[0065] Main steps: Rats in the modeling group were anesthetized with an intraperitoneal injection of 10% chloral hydrate (350 mg / kg) and fixed in the supine position on a mouse board. The skin and muscles of the neck were incised, and the common carotid artery was isolated. The rats were then fixed on the mouse board in the left lateral position. Under a surgical microscope, the skin was incised along the midpoint of the line connecting the external auditory canal and the canthus to expose the zygomatic arch. A small distractor was used to separate the distance between the zygomatic bone and the mandible. A bone window was created at the base of the skull, and the dura mater was torn to expose the right middle cerebral artery segment between the olfactory tract and the inferior cerebral vein. A small piece of plastic film was placed to protect the surrounding tissue. A small piece of quantitative filter paper soaked in 10 μL of 50% ferric chloride solution was applied to the segment of the middle cerebral artery. After 30 minutes, the filter paper was removed, the local tissue was rinsed with normal saline, and the wound was sutured layer by layer. The rats were returned to their cages for maintenance. The temperature was strictly controlled at 24-25°C during and after the operation. The sham group was treated the same as the modeling group, except that normal saline was applied to the middle cerebral artery.

[0066] After modeling, the rats were randomly divided into model control group, experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, and control group 4, with 6 rats in each group.

[0067] The experimental groups and gavage schedule are shown in Table 1.

[0068] Table 1 Oral gavage regimen of rats in each group The drug-treated group was given the drug by gavage 30 minutes after surgery, once a day for 2 consecutive weeks.

[0069] Determination of neurological dysfunction in rats The scoring criteria for neurological symptoms were as follows: 0 points, normal limbs and free walking; 1 point, the rat can walk normally, but when the rat is lifted and suspended in the air, the right forelimb cannot be fully extended, and is flexed, raised, and the shoulder is adducted; 2 points, based on the symptoms of 1 point, there is forward paralysis and signs of right-sided rotation walking; 3 points, based on the symptoms of 2 points, there is lateral push and easy falling, and walking is more difficult; 4 points, the rat cannot move independently, is paralyzed, and has partial loss of consciousness.

[0070] According to the above standards, the full score is 10 points. The higher the score, the more severe the brain dysfunction, which is used as an indicator of brain dysfunction.

[0071] Determination of cerebral infarction area in rats After behavioral scoring, the rats were decapitated and their brains removed. The olfactory brain, lower brainstem, and cerebellum were removed, and the remaining portion was immediately weighed wet. The brain was then cut coronally on ice into five slices of approximately equal thickness. The slices were incubated in TTC dye at 37°C for 30 minutes. Normal brain tissue appears rose red, while infarcted areas appear white. The brain slices were fixed in 10% formalin, and the white tissue was removed and weighed. The infarct area was calculated as the percentage of infarct weight to total brain weight.

[0072] Determination of brain tissue water content After decapitation, the rats were decapitated and the brains were removed. The left and right hemispheres were separated and weighed wet. The brains were then placed in a 110°C oven for 24 hours, and their dry weights were immediately measured. Brain water content = (wet weight - dry weight) / wet weight x 100%.

[0073] Determination of blood-brain barrier permeability Before sacrifice, rats were injected with 2% Evans blue (EB) via the femoral vein. Twenty minutes later, the brains were rapidly decapitated and the left and right cerebral hemispheres separated. The arachnoid membrane, blood clots, and choroid plexus of the ventricles were removed. The brains were then placed in a known volume of formamide for determination of brain volume. Formamide was added to a volume equal to five times the brain volume. The rats were placed in a 37°C water bath for 48 hours, and the optical density was measured at a wavelength of 632 nm. Formamide was used as a colorimetric blank, and EB content was calculated using a standard curve.

[0074] 2. Statistical methods GraphPad Prism 7.0 software was used for data processing and the quantitative data were The comparison between groups was performed with one-way analysis of variance, and the comparison between two groups was performed with LSD-t test. The difference was considered statistically significant when P < 0.05.

[0075] 3. Experimental Results 3.1 Effects on neurological dysfunction in rats The rats in the sham-operated group did not show any behavioral abnormalities, while the rats in the model control group showed obvious neurological dysfunction, mainly manifested as slow movement, inability to fully extend the right forelimb, and only being able to rotate to the right. Some rats were prone to falling when pushed sideways.

[0076] Compared with the model control group, the neurological dysfunction scores of rats in experimental groups 1, 2 and 3 were significantly reduced, indicating that the Chinese medicine composition of the present invention can significantly alleviate the neurological dysfunction of model rats. Compared with the control groups 1-4, there were significant differences in improving neurological dysfunction in experimental groups 1, 2 and 3. Figure 1 .

[0077] 3.2 Effects on cerebral infarction area in rats Compared with the model control group, the cerebral infarction area of ​​rats in Experimental Groups 1, 2, and 3 was significantly reduced, indicating that the Chinese medicine composition of the present invention can significantly reduce the degree of infarction in model rats. Compared with Control Groups 1-4, Experimental Groups 1, 2, and 3 showed significant differences in reducing cerebral infarction area. The results are shown in Table 2.

[0078] Table 2 Comparison of cerebral infarction area in each group ( , n=6) Note: Different lowercase letters in the same column indicate significant differences among different groups (P < 0.05).

[0079] 3.3 Effects on the permeability of the rat blood-brain barrier Compared with the sham-operated group, the EB content in the model control group was significantly increased.

[0080] Compared with the model control group, the EB content of rats in experimental group 1, experimental group 2, and experimental group 3 was significantly reduced, indicating that the Chinese medicine composition of the present invention can significantly reduce the permeability of the blood-brain barrier. Compared with the control groups 1-4, experimental group 1, experimental group 2, and experimental group 3 showed significant differences in reducing EB content. Figure 2 .

[0081] 3.4 Effects on rat brain water content Compared with the sham-operated group, the brain water content of rats in the model control group was significantly increased.

[0082] Compared with the model control group, the brain water content of rats in experimental group 1, experimental group 2, and experimental group 3 was significantly reduced, indicating that the Chinese medicine composition of the present invention can significantly reduce brain edema. Compared with the control groups 1-4, experimental group 1, experimental group 2, and experimental group 3 had significant differences in reducing brain water content. Figure 3 .

[0083] Comparative Example 1 does not contain Chuanxiong and Eupatorium, Comparative Example 2 does not contain Hirudo and Carapax Trionycis, Comparative Example 3 does not contain Salvia miltiorrhiza and Astragalus, and Comparative Example 4 changes the dosage ratio of the Chinese medicine composition. It can be seen from the data of the control group that only by adopting the complete formula of the present invention can the best therapeutic effect be achieved. If some of the components are discarded or the compatibility ratio is changed, the therapeutic effect of the composition will be significantly affected. The components of the present invention synergize with each other.

[0084] The above embodiments do not limit the present invention in any form. For those skilled in the art, any modifications, replacements, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A Chinese medicine composition for treating cerebral embolism, characterized in that: The traditional Chinese medicine composition for treating cerebral embolism comprises the following raw materials by weight: 15-25 parts of Chuanxiong, 15-25 parts of Cyperus rotundus, 15-25 parts of Hirudo, 12-18 parts of turtle shell, 15-25 parts of stir-fried chicken gizzard lining, 12-18 parts of Cyathula, 12-18 parts of Cistanche deserticola, 12-18 parts of Salvia miltiorrhiza, 10-15 parts of Astragalus, 10-15 parts of Citrus aurantium, 15-25 parts of Magnolia officinalis, 12-18 parts of Cornus officinalis, 15-20 parts of Acorus calamus and 8-12 parts of Piper mesenteriae.

2. The Chinese medicine composition for treating cerebral embolism according to claim 1, characterized in that The traditional Chinese medicine composition for treating cerebral embolism is prepared from the following components by weight: 20 parts of Chuanxiong, 20 parts of Cyperus rotundus, 20 parts of Hirudo, 15 parts of turtle shell, 20 parts of stir-fried chicken gizzard lining, 15 parts of Cyathula, 15 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 12 parts of Astragalus, 12 parts of Citrus aurantium, 20 parts of Magnolia officinalis, 15 parts of Cornus officinalis, 18 parts of Acorus calamus and 10 parts of Piper mesenteriae.

3. The Chinese medicine composition for treating cerebral embolism according to claim 1, characterized in that: The traditional Chinese medicine composition for treating cerebral embolism is prepared from the following raw materials, by weight: 15 parts of Chuanxiong, 25 parts of Cyperus rotundus, 15 parts of Hirudo, 18 parts of Carapax tricolor, 25 parts of stir-fried chicken gizzard lining, 18 parts of Cyathula capitata, 18 parts of Cistanche deserticola, 18 parts of Salvia miltiorrhiza, 15 parts of Astragalus membranaceus, 15 parts of Citrus aurantium, 25 parts of Magnolia officinalis, 12 parts of Cornus officinalis, 20 parts of Acorus calamus and 8 parts of Piper mesenteriae.

4. The Chinese medicine composition for treating cerebral embolism according to claim 1, characterized in that The traditional Chinese medicine composition for treating cerebral embolism is prepared from the following raw materials, by weight: 25 parts of Chuanxiong, 15 parts of Cyperus rotundus, 25 parts of Hirudo, 12 parts of turtle shell, 15 parts of stir-fried chicken gizzard lining, 12 parts of Cyathula, 12 parts of Cistanche deserticola, 12 parts of Salvia miltiorrhiza, 10 parts of Astragalus, 10 parts of Citrus aurantium, 15 parts of Magnolia officinalis, 18 parts of Cornus officinalis, 15 parts of Acorus calamus and 12 parts of Piper drench.

5. The method for preparing the Chinese medicine composition for treating cerebral embolism according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: weighing raw materials according to a formula, mixing them, adding water 7-9 times the weight of the mixture to the mixture, soaking for 1.5-2 hours, decocting the obtained soaking liquid to 110-130° C., cooling to 90-110° C., decocting for 0.5-1.5 hours, and filtering to obtain a first decoction and medicinal residues; adding water 5-7 times the weight of the medicinal residues to the medicinal residues, then boiling to 110-130° C., cooling to 90-110° C., decocting for 0.5-1.5 hours, and filtering to obtain a second decoction; and mixing the first decoction and the second decoction, concentrating, filtering, and vacuum drying to obtain the medicinal residue.

6. A Chinese medicine preparation for treating cerebral embolism, characterized in that: The traditional Chinese medicine preparation is prepared using the traditional Chinese medicine composition for treating cerebral embolism according to any one of claims 1 to 4 as a raw material.

7. The Chinese medicine preparation of the Chinese medicine composition for treating cerebral embolism according to claim 6, characterized in that: The traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

8. The Chinese medicine preparation of the Chinese medicine composition for treating cerebral embolism according to claim 6 or 7, characterized in that: The dosage form of the Chinese medicine preparation is Chinese medicine decoction, decoction, granules, pills, capsules, tablets, tea, suspension, powder or oral liquid.

9. The Chinese medicine preparation of the Chinese medicine composition for treating cerebral embolism according to claim 8, characterized in that: The dosage form of the Chinese medicine preparation is tablets.

10. Use of the traditional Chinese medicine composition for treating cerebral embolism according to any one of claims 1 to 4 in the preparation of a drug for preventing or treating cerebral embolism.

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