Positioning blowdown device for mouse intrauterine embryo electrotransfection experiment
By introducing an integrated steel plate structure and clamping claw design into the mouse intrauterine embryo electroporation experimental device, the problem of sewage flowing everywhere was solved, single-person operation and efficient sewage collection were achieved, and the experimental efficiency and safety were improved.
Patent Information
- Application Number
- CN202510904155.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-01
- Publication Date
- 2025-10-28
AI Technical Summary
The existing mouse intrauterine embryo electroporation experimental equipment lacks a systematic diversion and collection design, resulting in sewage flowing everywhere, increasing the difficulty of cleaning and requiring multiple people to operate, affecting the experimental environment and efficiency.
An integrated steel plate structure including sewage discharge components, guide plates, support parts and sewage collection parts was designed. Combined with slope and diversion design, it was formed by stamping process and equipped with clamping claws and fetal mouse fixers to achieve fast and orderly sewage collection and single-person operation.
It achieves rapid and orderly collection of sewage, reduces manual cleaning workload, lowers pollution risks, improves the convenience and efficiency of experimental operations, adapts to mice of different sizes, and ensures stable fixation of fetal mice.
Smart Images
Figure CN120837233A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biomedical device technology, and in particular to a positioning and waste disposal device for an intrauterine electroporation experiment of mouse embryos. Background Technology
[0002] The intrauterine electroporation experiment in mice involves injecting plasmid DNA into the ventricles of the fetal mouse brain, then applying voltage to a suitable location outside the uterine wall. Under the influence of the electric field, the plasmid DNA migrates towards the positive electrode plate, eventually reaching the target brain region. This method allows for the transfection of genes expressing different colored fluorescent proteins into brain tissue to observe cell migration and differentiation, thereby analyzing the gene's function. However, the experiment often requires at least two people to operate, performing tasks such as slowly injecting the plasmid, pricking the electrode, and wetting the embryo, making it impossible to perform the experiment alone and increasing manpower consumption. Furthermore, during delicate operations, operators often damage the lateral ventricle tissue or misplace the injection site due to poor embryo positioning. The continuous dripping of warm saline to wet the embryo during the experiment leads to the accumulation of wastewater on the worktable after repeated operations. Existing devices typically only have simple openings at the bottom of the worktable, lacking a systematic design for drainage and collection. This crude treatment method allows wastewater to initially collect under gravity, but its flow path cannot be effectively controlled. During the experiment, wastewater can easily seep and spread along the cracks and holes on the surface of the experimental table, and may even drip directly onto the workbench below through the openings, which not only contaminates the surrounding equipment, but also increases the difficulty of cleaning. Therefore, this application provides a positioning and wastewater discharge device for mouse intrauterine embryo electroporation experiments to meet the needs. Summary of the Invention
[0003] The technical problem this invention aims to solve is to provide a positioning and wastewater discharge device for mouse intrauterine embryo electroporation experiments. By incorporating a wastewater discharge component, a guide plate, a support section, and a wastewater collection section into an integrated steel plate structure, and utilizing slope and flow guidance design, it can quickly and systematically collect wastewater generated during the experiment, preventing wastewater from flowing around and affecting the experimental environment. Simultaneously, the wastewater discharge component is integrally formed using a stamping process, resulting in a simple overall structure, low manufacturing difficulty, and controllable cost. Furthermore, the symmetrically arranged gripping claws allow for flexible spacing adjustment via a crank handle, accommodating mice of different sizes. A fetal mouse fixation device is located at the top of the gripping claws, ensuring that neither the mother mouse nor the fetus moves during the experiment. The operator can use one hand to push the syringe and the other to hold the electrode for electroporation of the mouse embryo. This addresses the problems of existing positioning and wastewater discharge devices for mouse intrauterine embryo electroporation experiments that only use a bottom opening and lack a systematic flow guidance and collection design, while also requiring at least two people to cooperate in the experiment, increasing manpower consumption.
[0004] To solve the above-mentioned technical problems, the present invention provides the following technical solution:
[0005] A positioning and waste removal device for an electroporation experiment of mouse uterine embryos includes an experimental table, a tension strap fixedly connected to the top of the experimental table, and a mechanical micro-propeller inserted inside the tension strap.
[0006] A magnifying glass is fixedly connected to the top of the experimental table, a saline drip device is fixedly connected to the top of the experimental table, a wastewater storage tank is provided on the top of the experimental table, and a wastewater discharge component is used to collect wastewater generated during the experiment. The wastewater discharge component is connected to the experimental table.
[0007] Optionally, the sewage discharge assembly includes a guide plate and a support portion, both of which are fixedly connected to the top of the experimental platform. A connecting portion is fixedly connected between the guide plate and the support portion. Sewage collection portions are fixedly connected to both sides of the support portion and the side of the guide plate near the support portion. A sewage discharge port is provided at the bottom of the sewage collection portion, and a sewage discharge pipe is fixedly connected to the bottom of the sewage discharge port.
[0008] Optionally, the guide plate, support, connecting part, and sewage collection part are integrated into one structure, and the sewage pipe is provided with a through hole at the corresponding position of the experimental platform.
[0009] Optionally, a sludge storage tank is fitted inside the sludge storage tank, and a guide plate is provided on the top of the sludge storage tank.
[0010] Optionally, the guide plate and the sludge storage tank are an integrated structure, and the thickness of the guide plate is less than the thickness of the sludge storage tank.
[0011] Optionally, the dirt collection part is slidably connected to a clamping claw, the bottom of the clamping claw is fixedly connected to a connecting plate, the bottom of the connecting plate is provided with a guide groove, the top of the clamping claw is provided with a sliding groove, and the inner side of the clamping claw is provided with a corrugated rubber sheet.
[0012] Optionally, a rotating rod is rotatably connected inside the experimental platform, a crank is fixedly connected to the end of the rotating rod away from the experimental platform, a connecting block is threaded onto the surface of the rotating rod, and guide rods are fixedly connected to both sides of the connecting block.
[0013] Optionally, a mouse retainer is slidably connected inside the groove, a flexible sheet is fixedly connected to the inner side of the mouse retainer, a locking block is fixedly connected to one end of the mouse retainer near the flexible sheet, and a groove is formed on the inner wall of the mouse retainer.
[0014] Optionally, the outer contour of the guide rod matches the inner contour of the guide groove, and the guide rod is slidably connected inside the guide groove.
[0015] Optionally, the output end of the mechanical micro-pusher is fitted with a flexible tube, and a clamp is slidably connected to the surface of the flexible tube. The surface of the clamp is provided with weakened grippers, and a glass electrode is engaged inside the weakened grippers.
[0016] Compared with the prior art, the present invention has at least the following beneficial effects:
[0017] In the above solution, by setting up a sewage discharge component, an integrated steel plate structure of guide plate, support and sewage collection part, and utilizing slope and flow guidance design, it can quickly and orderly collect sewage such as continuously dripping physiological saline and blood flowing out of dissected mice during the experiment, preventing sewage from flowing around and affecting the experimental environment. At the same time, the sewage discharge component is integrally formed by stamping process, with simple overall structure, low manufacturing difficulty and controllable cost. In addition, the clever cooperation between sewage tank and guide plate, when sewage tank is removed, guide plate automatically scrapes the inner wall of sewage tank, realizing synchronous cleaning of sewage tank, reducing the workload and difficulty of manual cleaning, and also reducing the risk of pollution caused by stain residue.
[0018] The symmetrically arranged grippers allow for flexible spacing adjustment via a crank handle, accommodating mice of different sizes. The inner wavy rubber pad fits snugly against the mouse's body, providing stable fixation while the softness of the rubber prevents secondary damage to the dissected mouse, ensuring the safety of the experimental subject. Simultaneously, the fetal mouse restraint device, which slides to the top of the grippers, can fix the fetal mouse exposed to the outside, ensuring its stability during the experiment and laying the foundation for subsequent precise operations.
[0019] The designed tension straps facilitate quick and easy fixation of the mechanical micro-pump, ensuring that excessive force during plasmid injection does not damage the lateral ventricle tissue of the fetal mice, while also reducing installation time. The movable magnifying glass can be precisely adjusted to the mouse uterus, providing a clear field of view. The holder can slide flexibly, facilitating the adjustment of the glass electrode position, making needle insertion and plasmid injection operations more precise and efficient. The overall design optimizes the experimental process, improving the convenience and efficiency of experimental operations. Furthermore, since both the mother and fetal mice are fixed, only one person is needed to perform plasmid delivery and needle electrode insertion operations during the experiment. Attached Figure Description
[0020] The accompanying drawings, which are incorporated herein and form part of the specification, illustrate embodiments of the invention and, together with the specification, further serve to explain the principles of the invention and enable those skilled in the art to practice and use the invention.
[0021] Figure 1 A schematic diagram of the overall structure of the positioning and waste removal device used in the electroporation experiment of mouse uterine embryos;
[0022] Figure 2 A schematic cross-sectional view of a positioning and waste removal device used in an intrauterine electroporation experiment of mouse embryos.
[0023] Figure 3 This is a magnified view of the structure at point A;
[0024] Figure 4 This is a schematic diagram of the cross-sectional structure of the dirt collection part and the clamping claws.
[0025] Figure 5 This is a magnified structural diagram of point B;
[0026] Figure 6 This is a schematic diagram of the clamping component structure;
[0027] Figure 7 This is a schematic diagram of the enlarged cross-sectional view of the sludge storage tank;
[0028] Figure 8 This is a schematic diagram of the mechanical micro-propeller and the hose mating structure.
[0029] Figure 9 This is an enlarged structural schematic diagram of the gripper;
[0030] Figure 10 This is an enlarged structural diagram of the fetal mouse restraint device.
[0031] Figure label:
[0032] 1. Experimental table; 101. Tightening strap; 102. Magnifying glass; 103. Physiological saline drip device; 2. Guide plate; 201. Connecting part; 202. Support part; 203. Sewage collection part; 204. Sewage outlet; 205. Sewage pipe; 3. Sewage tank; 301. Sewage bucket; 302. Guide plate; 4. Clamping claw; 401. Connecting plate; 402. Guide groove; 403. Slide groove; 5. Handle; 501. Rotating rod; 502. Connecting block; 503. Guide rod; 6. Mechanical micro-propeller; 7. Tube; 8. Clamping device; 801. Weakened clamping claw; 802. Glass electrode; 9. Fetal mouse fixation device; 901. Flexible film; 902. Locking block; 903. Groove.
[0033] As shown in the figure, specific structures and devices are marked in the figure to clearly illustrate the structure of the embodiments of the present invention. However, this is only for illustrative purposes and is not intended to limit the present invention to this specific structure, device and environment. Those skilled in the art can adjust or modify these devices and environments according to specific needs. Detailed Implementation
[0034] The following is a detailed description of a positioning and waste removal device for electroporation experiments of mouse intrauterine embryos provided by the present invention, with reference to the accompanying drawings and specific embodiments. It should be noted that, to make the embodiments more detailed, the following embodiments are the best and preferred embodiments; those skilled in the art can also use other alternative methods to implement some known technologies; and the accompanying drawings are only for more specific description of the embodiments and are not intended to specifically limit the present invention.
[0035] It should be noted that the use of terms such as "an embodiment," "an embodiment," "an exemplary embodiment," and "some embodiments" in the specification indicates that the described embodiment may include a specific feature, structure, or characteristic, but not every embodiment necessarily includes that specific feature, structure, or characteristic. Furthermore, when a specific feature, structure, or characteristic is described in connection with an embodiment, implementing such a feature, structure, or characteristic in conjunction with other embodiments (whether explicitly described or not) should be within the knowledge of those skilled in the art.
[0036] Generally, terms can be understood at least partly from their use in context. For example, depending at least partly on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in a singular sense, or a combination of features, structures, or characteristics in a plural sense. Additionally, the term "based on" can be understood not necessarily to convey an exclusive set of factors, but rather, alternatively, depending at least partly on the context, to allow for the presence of other factors that are not necessarily explicitly described.
[0037] It is understood that the meanings of “on”, “above”, and “above” in this invention should be interpreted in the broadest manner, such that “on” means not only “directly on” something, but also includes the meaning of being “on” something with an intervening feature or layer, and that “above” or “above” means not only “on” something, but also includes the meaning of being “on” something without an intervening feature or layer.
[0038] Furthermore, spatially related terms such as “below,” “under,” “lower,” “above,” and “upper” are used herein for convenience to describe the relationship of one element or feature to one or more other elements or features, as illustrated in the accompanying drawings. Spatially related terms are intended to cover different orientations in the use or operation of the device other than those depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially related descriptive terms used herein can be interpreted similarly.
[0039] like Figure 1 and Figure 9As shown, an embodiment of the present invention provides a positioning and waste removal device for an intrauterine electroporation experiment of mouse embryos, including an experimental table 1. A tension strap 101 is fixedly connected to the top of the experimental table 1, and a mechanical micro-pump 6 is inserted inside the tension strap 101. The mechanical micro-pump is existing technology and can ensure the injection speed and ensure that the injection force is not too large to avoid damaging the lateral ventricle tissue of the fetal mouse and affecting the experimental process. A magnifying glass 102 is fixedly connected to the top of the experimental table 1, and a saline drip device 103 is fixedly connected to the top of the experimental table 1. The saline drip device 103 is existing technology and is mainly used for holding... 37°C physiological saline was continuously dripped into the embryos to moisten them. A waste collection tank 3 was provided on the top of the experimental table 1. A waste discharge assembly was also provided to collect the wastewater generated during the experiment. The waste discharge assembly was connected to the experimental table 1. By setting up the waste discharge assembly and designing the guide plate 2, support 202 and waste collection part 203 as an integrated steel plate structure, the wastewater generated during the experiment can be collected quickly and orderly by using the slope and flow guidance design, preventing the wastewater from flowing around and affecting the experimental environment. At the same time, the waste discharge assembly was integrally formed by stamping process, with a simple overall structure, low manufacturing difficulty and controllable cost.
[0040] like Figures 1 to 5 As shown, the sewage discharge assembly includes a guide plate 2 and a support portion 202. Both the guide plate 2 and the support portion 202 are fixedly connected to the top of the experimental platform 1. The support portion 202 has a curved plate structure, with the height of the two sides lower than the height of the middle. The height of the guide plate 2 near the support portion 202 is lower than the height of the side away from the support portion 202. Sewage collection portions 203 are fixedly connected to both sides of the support portion 202 and the guide plate 2 near the support portion 202. Sewage generated during the experiment will flow through the guide plate 2 to the inner sewage collection portion 203, while sewage on the surface of the support portion 202 will... The sewage flows to the two sides of the collection section 203. The side cross-section of the collection section 203 is a semi-circular structure, which can collect the sewage guided by the guide plate 2 and the support section 202 into the collection section 203. The bottom of the collection section 203 is provided with a sewage outlet 204. The bottom of the sewage outlet 204 is fixedly connected to a sewage pipe 205, which can discharge the sewage inside the collection section 203 from the sewage pipe 205. The guide plate 2, the support section 202, the connecting section 201 and the collection section 203 are integrated structures. The whole is made of steel plate and is formed by stamping process. The structure is simple and easy to produce.
[0041] like Figure 7As shown, a sludge collection tank 301 is fitted inside the sludge collection tank 3. A guide plate 302 is provided on the top of the sludge collection tank 301. A through hole is opened at the corresponding position of the sewage pipe 205 and the experimental table 1. The through hole is located above the guide plate 302. The sewage discharged from the sewage pipe 205 can be guided by the guide plate 302 and thus collected into the sludge collection tank 301. The guide plate 302 and the sludge collection tank 301 are an integrated structure, made of plastic and injection molded. The thickness of the guide plate 302 is lower than the thickness of the sludge collection tank 301. The tension of its material allows the guide plate 302 to abut against the surface of the sludge collection tank 3. When the sludge collection tank 301 is removed, the guide plate 302 can scrape the surface of the sludge collection tank 3, collecting the residual dirt on the surface of the sludge collection tank 3 into the sludge collection tank 301.
[0042] like Figures 4 to 6 As shown, the dirt collection part 203 has two slidably connected clamping claws 4 inside, which are symmetrically arranged on both sides of the central axis of the experimental table 1. The dirt collection part 203 is made of steel plate and has a certain tension, which can guide and clamp the clamping claws 4. A connecting part 201 is fixedly connected between the guide plate 2 and the support part 202. The contours of the two sides of the connecting part 201 are consistent with the contours of the dirt collection part 203. The middle part adopts a curved plate connection for limiting the clamping claws 4. A connecting plate 401 is fixedly connected to the bottom of the clamping claws 4. A guide groove 402 is opened at the bottom of the connecting plate 401. A rotating rod 501 is rotatably connected inside the experimental table 1. A crank 5 is fixedly connected to the end of the rotating rod 501 away from the experimental table. A connecting block is threadedly connected to the surface of the rotating rod 501. 502. Guide rods 503 are fixedly connected to both sides of the connecting block 502. Turning the crank handle 5 causes the rotating rod 501 to rotate, thereby causing the connecting block 502 to move back and forth on the surface of the rotating rod 501. The outer contour of the guide rod 503 matches the inner contour of the guide groove 402. The guide rod 503 is slidably connected inside the guide groove 402. The back and forth movement of the guide rod 503 inside the guide groove 402 can drive the connecting block 502 and the gripping claw 4 to move inward. The distance between the gripping claw 4 can be adjusted to accommodate mice of different sizes. A wavy rubber sheet is provided on the inner side of the gripping claw 4. When in contact with the mouse, the rubber sheet will change shape to fit the mouse tightly. Due to its material properties, it will not cause secondary damage to the dissected mouse when gripping it.
[0043] like Figures 8 to 9 As shown, a flexible tube 7 is connected to the output end of the mechanical micro-pump 6. A needle is installed inside the end of the flexible tube 7 furthest from the mechanical micro-pump 6. In experiments, plasmids inside the mechanical micro-pump 6 can be injected into the ventricle of a fetal rat through the flexible tube 7. A clamp 8 is slidably connected to the surface of the flexible tube 7. The clamp 8 has weakened grippers 801 on its surface, and a glass electrode 802 is engaged inside the weakened grippers 801. Moving the clamp 8 changes the position of the glass electrode 802 on the surface of the flexible tube 7. The weakened grippers 801 can be made of a flexible material to avoid damaging the glass electrode 802.
[0044] like Figure 4 and Figure 10 As shown, the top of the gripper 4 has a groove 403, and a fetal mouse fixation device 9 is slidably connected inside the groove 403. The movable fetal mouse fixation device 9 can ensure that fetal mice in different positions can be fixed. A flexible sheet 901 is fixedly connected to the inner side of the fetal mouse fixation device 9. The flexible sheet 901 is made of medical rubber to avoid damage to the fetal mouse when fixing it. A buffer groove is formed between the flexible sheet 901 and the fetal mouse fixation device 9 to ensure that fetal mice of different sizes can be fixed, and at the same time to ensure that the fetal mouse does not move during the experiment, so as to avoid the electrode position from shifting relative to the ventricle during electroporation, which would result in unsuccessful electroporation. There are two fetal mouse restrainers 9, which are respectively set inside the two clamping claws 4. One fetal mouse restrainer 9 has a locking block 902 fixedly connected to one end near the flexible film 901. The other fetal mouse restrainer 9 has a locking groove at the contact position, and the locking block 902 is locked inside the locking groove. The inner wall of the fetal mouse restrainer 9 has a groove 903. When the mother mouse is too small, resulting in the fetal mouse being too small, if the clamping claws 4 are moved, the fetal mouse restrainer 9 will deform at the groove 903. The structures on both sides of the groove 903 will expand outward, thus expanding to both sides. At this time, the internal area of the flexible film 901 will shrink to ensure that the smaller fetal mouse can be fixed.
[0045] The workflow of the technical solution of this invention is as follows:
[0046] In use, anesthetized mice are placed steadily on top of the experimental table 1. The gripping claw 4 is moved inward by rotating the handle 5, and its inner wavy rubber sheet gently and securely fixes the mouse. This design effectively avoids secondary damage to the dissected mouse. As the gripping claw 4 moves, the fetal mouse restraint 9 closes, allowing it to be moved to the mouse's uterine region. After mouse fixation, the mechanical micro-particle pusher 6, loaded with plasmids, is fixed to the top of the experimental table 1 using the strap 101. Next, the magnifying glass 102 is precisely moved to the mouse's uterine region, providing a clear view for subsequent operations. Dissection is then performed, fully exposing the mouse's uterus. Gently pressing the uterine region forces the fetal mouse to be exposed, placing it inside the fetal mouse restraint 9. The flexible sheet 901 effectively secures the fetal mouse. At this point, the saline drip device 103 can be turned on to continuously drip warm saline to moisten the fetal mice. Next, holding the clamp 8, accurately insert the needle at one end of the tubing 7 into the ventricle of the fetal mouse. Then, by sliding the clamp 8, flexibly adjust the position of the glass electrode 802 to precisely position it at the mouse's uterine wall. At this time, smoothly push the mechanical micro-propeller 6 to inject the plasmid into the ventricle of the fetal mouse at a uniform speed through the tubing 7, and start the electrode to formally carry out the mouse embryo electroporation experiment.
[0047] After the experiment, carefully remove the needle to complete the entire electroporation experiment. During the experiment, the continuously dripping saline solution and blood from dissected mice, along with other wastewater, will be guided by the guide plate 2 and the support 202 to flow orderly into the collection section 203, and finally discharged into the sludge tank 301 through the drain pipe 205 at the bottom of the collection section 203. When cleaning the sludge tank 301 after the experiment, simply lift the sludge tank 301 upwards. Because the guide plate 302 is tightly abutted against the sludge tank 3, it will automatically scrape the inner wall of the sludge tank 3 during the removal of the sludge tank 301, achieving simultaneous cleaning of the inner wall of the sludge tank 3.
[0048] This invention encompasses any substitutions, modifications, equivalent methods, and solutions made within the spirit and scope of this invention. To provide the public with a thorough understanding of this invention, specific details are described in detail in the following preferred embodiments; however, those skilled in the art will fully understand the invention even without these details. Furthermore, to avoid unnecessary misunderstanding of the essence of this invention, well-known methods, processes, procedures, components, and circuits are not described in detail.
[0049] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A positioning and waste removal device for an intrauterine embryo electroporation experiment in mice, comprising an experimental table, characterized in that, A tension strap is fixedly connected to the top of the experimental platform, and a mechanical micro-propeller is inserted inside the tension strap; A magnifying glass is fixedly connected to the top of the experimental table, a saline drip device is fixedly connected to the top of the experimental table, and a waste collection tank is opened on the top of the experimental table. A wastewater discharge assembly is used to collect wastewater generated during the experiment, and the wastewater discharge assembly is connected to the experimental platform.
2. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 1, characterized in that, The sewage discharge assembly includes a guide plate and a support part, both of which are fixedly connected to the top of the experimental platform. A connecting part is fixedly connected between the guide plate and the support part. Sewage collection parts are fixedly connected to both sides of the support part and the side of the guide plate near the support part. A sewage discharge port is opened at the bottom of the sewage collection part, and a sewage discharge pipe is fixedly connected to the bottom of the sewage discharge port.
3. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 2, characterized in that, The guide plate, support, connecting part, and sewage collection part are integrated into one structure, and the sewage pipe has a through hole at the corresponding position of the experimental table.
4. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 1, characterized in that, The sludge storage tank is fitted with a sludge storage bucket inside, and a guide plate is provided on the top of the sludge storage bucket.
5. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 4, characterized in that, The guide plate and the sludge storage tank are integrated into one structure, and the thickness of the guide plate is less than the thickness of the sludge storage tank.
6. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 2, characterized in that, The dirt collection section is slidably connected to a clamping claw. A connecting plate is fixedly connected to the bottom of the clamping claw. A guide groove is provided at the bottom of the connecting plate. A sliding groove is provided at the top of the clamping claw. A wavy rubber sheet is provided on the inner side of the clamping claw.
7. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 1, characterized in that, The experimental platform is rotatably connected to a rotating rod. A crank is fixedly connected to the end of the rotating rod away from the experimental platform. A connecting block is threaded onto the surface of the rotating rod, and guide rods are fixedly connected to both sides of the connecting block.
8. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 6, characterized in that, A mouse retainer is slidably connected inside the groove. A flexible sheet is fixedly connected to the inner side of the mouse retainer. A locking block is fixedly connected to one end of the mouse retainer near the flexible sheet. A groove is formed on the inner wall of the mouse retainer.
9. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 7, characterized in that, The outer contour of the guide rod matches the inner contour of the guide groove, and the guide rod is slidably connected inside the guide groove.
10. The positioning and waste removal device for mouse intrauterine embryo electroporation experiment according to claim 1, characterized in that, The mechanical micro-propeller output end is fitted with a flexible tube, and a clamp is slidably connected to the surface of the flexible tube. The surface of the clamp is provided with weakened grippers, and a glass electrode is engaged inside the weakened grippers.