Skin care composition for eczema based on sebum film bionics as well as preparation method and application of skin care composition

Abstract

The invention discloses a skin care composition for eczema based on sebum membrane bionics as well as a preparation method and application of the skin care composition for eczema based on sebum membrane bionics, the skin care composition for eczema based on sebum membrane bionics comprises active ingredients such as rosa palmifolia fruit oil, oat kernel oil, ceramide, phytosterol oleate, simmondsia chinensis seed oil and squalane, the mass ratio of the rumex mairei fruit oil to the oat kernel oil to the ceramide to the phytosterol oleate to the simmondsia chinensis seed oil to the squalane is (0.005 to 5) to (0.02 to 20) to (0.001 to 5) to (0.001 to 20) to (0.01 to 15) to (0.01 to 15). By implementing the method, the lipid composition structure of a human body sebum film can be accurately simulated, the damaged skin barrier can be effectively repaired, the anti-inflammatory soothing characteristic is achieved, multiple protection is provided for the special pathological state of eczema skin, and the method can be widely applied to the field of cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a skin care composition for eczema based on biomimetic sebum film, its preparation method, and its application. Background Technology

[0002] Eczema is a skin inflammation caused by various internal and external factors, characterized by a marked tendency to ooze. Clinically, it is characterized by diverse skin lesions, intense itching, and a high recurrence rate. Atopic dermatitis, a type of eczema, is a chronic, recurrent, inflammatory skin disease related to a genetically predisposed allergic constitution, exhibiting a clear "atopic" pattern in the individual or family. Atopic dermatitis is the most common disease in infants and young children. Data shows that the incidence of atopic dermatitis in infants under one year old in my country is approximately 30%. Although antibiotics such as fusidic acid ointment and mupirocin ointment can be used for treatment, infants' skin is more sensitive and fragile. The use of antibiotics may cause visible telangiectasia in infants' skin within a short period. Furthermore, antibiotics also pose problems such as drug resistance and potential impact on subsequent immune development. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide a skin care composition for eczema based on biomimicry of the sebum membrane, which can accurately simulate the lipid composition structure of the human sebum membrane and effectively repair the damaged skin barrier.

[0004] Another technical problem to be solved by the present invention is to provide a method for preparing a skin care composition for eczema based on biomimetic sebum film.

[0005] Another technical problem that this invention aims to solve is to provide an application of a skin care composition for eczema based on biomimetic sebum film.

[0006] To address the aforementioned problems, this invention discloses a skincare composition for eczema based on biomimetic sebum film. The composition comprises morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane, wherein the mass ratio of morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane is (0.005–5):(0.02–20):(0.001–5):(0.001–20):(0.01–15):(0.01–15).

[0007] As an improvement to the above technical solution, the mass ratio of the morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil and squalane is (0.02-15):(0.002-5):(0.001-5):(0.001-15):(0.01-15):(0.01-15).

[0008] As an improvement to the above technical solution, the oat kernel oil is extracted using the following method:

[0009] Wax-ripe oat kernels are physically crushed, and then enzymatically hydrolyzed with a compound enzyme to obtain oat kernel hydrolysate.

[0010] The enzymatic hydrolysis product of the oat kernels was dried and pressed to extract crude oat kernel oil.

[0011] The crude oat kernel oil is degummed and deacidified to obtain the oat kernel oil.

[0012] As an improvement to the above technical solution, the amount of the compound enzyme added is 1% to 4% of the dry weight of the wax-ripe oat kernels;

[0013] The complex enzyme comprises cellulase, hemicellulase, pectinase, and protease; the mass ratio of cellulase, hemicellulase, pectinase, and protease is 1:(1-3):(1-2):(0.5-1);

[0014] The enzymatic hydrolysis time is 1h to 3h, the enzymatic hydrolysis temperature is 30℃ to 50℃, and the enzymatic hydrolysis pH is 4.5 to 5.5.

[0015] As an improvement to the above technical solution, the oil from the fruit of the palm tree is extracted using the following method:

[0016] Take the fruit of the palm tree, dry it and physically crush it to obtain palm fruit powder;

[0017] The powdered fruit of the Mauritius palm was subjected to supercritical extraction to separate and obtain the Mauritius palm oil.

[0018] As an improvement to the above technical solution, the temperature of supercritical extraction is 40℃~50℃, the pressure is 35MPa~45MPa, the time is 40min~70min, and the CO2 flow rate is 20L / h~30L / h.

[0019] The separation temperature is 30℃~40℃, and the pressure is 5MPa~10MPa.

[0020] As an improvement to the above technical solution, the ceramide includes one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG.

[0021] Accordingly, the present invention also discloses a method for preparing the above-mentioned biomimetic skin care composition for eczema, comprising the following steps:

[0022] The fruit oil of the palm, the kernel oil of the oat, the seed oil of the jojoba, the phytosterol oleate and the squalane were stirred at 40℃~55℃ until completely dissolved.

[0023] Lower the temperature to 30℃~35℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on the biomimetic sebum film.

[0024] As an improvement to the above technical solution, the following steps are included:

[0025] The fruit oil of the palm, the kernel oil of oats and the phytosterol oleate were stirred at 50℃~55℃ until completely dissolved to obtain the first component;

[0026] Jojoba seed oil, phytosterol oleate and squalane were stirred at 40℃~45℃ until completely dissolved to obtain the second component;

[0027] Mix the first component and the second component, lower the temperature to 30℃~35℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on the biomimetic sebum film.

[0028] The mass ratio of phytosterol oleate in the first component and the second component is 1:(0.3-0.5).

[0029] Accordingly, the present invention also discloses the application of the above-mentioned biomimetic eczema skin care composition based on sebum film in the preparation of cosmetics, wherein the cosmetics contain 5 wt% to 30 wt% of the above-mentioned biomimetic eczema skin care composition based on sebum film.

[0030] Implementing this invention has the following beneficial effects:

[0031] 1. This invention precisely mimics the lipid composition of the human sebum membrane through the synergistic formulation of morrhagic palm fruit oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane, effectively repairing the damaged skin barrier. Combined with the anti-inflammatory and soothing properties of oat kernel oil, it yields a skincare composition for adjuvant treatment and daily care of skin barrier dysfunction diseases such as eczema and atopic dermatitis. It is gentle and non-irritating, and is especially suitable for infants with atopic dermatitis.

[0032] 2. The skin care composition provided by the present invention includes plant oils such as mangrove fruit oil, oat kernel oil and jojoba seed oil. Jojoba seed oil has excellent compatibility with the skin and can improve the permeability of the product. Oat kernel oil and mangrove fruit oil have good anti-inflammatory and soothing properties, providing multiple protections for the special pathological condition of eczema skin. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in further detail below.

[0034] This invention provides a skin care composition for eczema based on a biomimetic sebum film, comprising morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane, wherein the mass ratio of morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane is (0.005–5):(0.02–20):(0.001–5):(0.001–20):(0.01–15):(0.01–15). Preferably, the mass ratio of morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil, and squalane is (0.002–5):(0.02–15):(0.001–5):(0.001–15):(0.01–15):(0.01–15).

[0035] Ceramides, as a major component of the intercellular lipids of the stratum corneum, not only act as second messenger molecules in the sphingomyelin pathway but also play a crucial role in the formation of the epidermal stratum corneum, possessing functions such as maintaining the skin barrier, moisturizing, anti-aging, whitening, and disease treatment. Ceramides include one or more combinations of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG. Ceramide EOP and ceramide NP can repair the sebum film and help renew the skin's natural protective layer, enhancing the skin's surface barrier function. Ceramide NS can repair the sebum film while inhibiting sebaceous gland secretion, maintaining the skin's water-oil balance. Ceramide AS, ceramide AP, and ceramide NG have the effect of repairing the skin barrier.

[0036] Phytosterol esters achieve internal hydration by repairing the lipid layer, ensuring sufficient moisture within the skin and maintaining a high-moisture environment for cells. This promotes active metabolism in the basal layer cells, accelerating the renewal of surface cells. The human skin lipid layer primarily contains cholesterol, free fatty acids, ceramides, and small amounts of other substances. When the skin is subjected to external physical or chemical stimuli or when the lipid layer's components are deficient, the lipid layer becomes damaged or lost, leading to impaired function of the stratum corneum in protecting the skin. Phytosterols and cholesterol have extremely similar molecular structures, and the sterol esters formed after esterification are more easily absorbed by the skin. Therefore, phytosterol esters can replace cholesterol as the best lipid layer repair ingredient. Furthermore, the monomeric component β-sitosterol ester in phytosterol esters has excellent anti-inflammatory effects. As a non-hormonal substance, it is non-addictive and non-irritating, and can help the skin eliminate inflammation caused by bacterial growth and internal metabolic disorders.

[0037] Squalane is a hydrocarbon-based oil derived from the hydrogenation of squalene. It is a natural occlusive agent, inert, non-toxic, and has excellent skin affinity, preventing skin allergies. It helps maintain the balance between the skin and sebum, deeply moisturizing while enhancing the skin's ability to absorb moisture and other nutrients. Long-term use can improve skin problems such as sagging, dryness, peeling, roughness, and dullness, helping the skin regain its softness. Squalane can also accelerate skin cell metabolism, repair damaged cells, and form a protective sebum film on the skin surface, helping to block external pollution and irritation, preventing or alleviating allergy symptoms. It is a safe ingredient for sensitive skin.

[0038] Oat kernel oil is rich in unsaturated fatty acids, such as oleic acid and linoleic acid. These components can penetrate deep into the skin, nourishing skin cells and helping to repair the damaged skin barrier. In addition, oat kernel oil contains antioxidants such as vitamin E, which can effectively resist free radical damage to the skin, reduce inflammation, and soothe itching and redness caused by eczema. Its gentle nature makes it suitable for sensitive skin and will not cause additional irritation.

[0039] Mauri palm oil is primarily composed of unsaturated fatty acids, rich in oleic acid, linoleic acid, palmitic acid, carotenoids, vitamin E, and vitamin C. Mauri palm oil has excellent moisturizing properties, penetrating deep into the skin to replenish moisture, prevent dryness, and thus alleviate eczema symptoms. It also has excellent permeability, allowing for rapid absorption by the skin, nourishing it, providing nutrients, and promoting skin cell repair and regeneration.

[0040] Jojoba seed oil has a molecular arrangement very similar to human fat, making it a highly stable and easily spreadable base oil. Rich in nutrients such as vitamins A, B, and E, as well as calcium and magnesium, it enhances the skin's immunity and repair capabilities, promoting the healing of eczema-damaged skin. It quickly penetrates and is absorbed into the skin, softening the stratum corneum, leaving the skin soft and elastic, while locking in moisture and reducing water loss, providing excellent moisturizing effects. Jojoba seed oil also has anti-inflammatory and antibacterial properties, effectively improving skin inflammation and eczema. Furthermore, its composition is similar to sebum, making it skin-friendly and non-allergenic, suitable for all skin types.

[0041] This invention precisely mimics the lipid composition of the human sebum membrane through a synergistic formulation of mangrove oil, ceramides, phytosterol oleate, jojoba seed oil, and squalane. This effectively repairs damaged skin barriers. Combined with the anti-inflammatory and soothing properties of oat kernel oil, it yields a skincare composition for adjunctive treatment and daily care of skin barrier dysfunction diseases such as eczema and atopic dermatitis, especially suitable for infants with atopic dermatitis. Jojoba seed oil has excellent skin compatibility, which can improve product permeability. Oat kernel oil and mangrove oil have excellent anti-inflammatory and soothing properties, providing multiple layers of protection against the specific pathological condition of eczema-prone skin.

[0042] In one embodiment, the oat kernel oil is extracted using the following method:

[0043] (1) Take oat kernels at the waxy ripening stage, physically crush them, add compound enzymes for enzymatic hydrolysis, and obtain oat kernel enzymatic hydrolysis products.

[0044] The wax-ripe oat kernels are physically crushed and sieved with a mesh size of 1mm to 3mm.

[0045] The amount of the compound enzyme added is 1% to 4% of the dry weight of the wax-ripe oat kernels, for example 1.5%, 2%, 2.5%, 3% or 3.5%, but not limited thereto.

[0046] The complex enzyme comprises cellulase, hemicellulase, pectinase, and protease; the mass ratio of cellulase, hemicellulase, pectinase, and protease is 1:(1-3):(1-2):(0.5-1). More preferably, the mass ratio of cellulase, hemicellulase, pectinase, and protease is 1:(1.5-2):(1.5-2):(0.6-0.8).

[0047] Cellulase can break down the cellulose microfiber skeleton in the cell wall of oat kernels, disintegrate the rigid structure of the cell wall, and release lipid bodies wrapped in cellulose.

[0048] Hemicellulase can degrade the hemicellulose network layer, increase cell wall porosity, remove steric hindrance to cellulase, and improve the enzymatic hydrolysis efficiency of cellulase.

[0049] Pectinase can dissolve pectin in the intercellular layer, separate cell monomers, reduce material viscosity, and improve the flowability of the pressed product.

[0050] Proteases can hydrolyze protein-lipid complexes, releasing bound lipids and improving lipid aggregation efficiency.

[0051] Enzymatic hydrolysis can break down cell walls and release lipids. Pre-treating oat kernels with enzymatic hydrolysis before pressing can shorten processing time and increase oil yield. Compared with direct pressing, the oil yield can be increased by 30% to 40%, and the processing time can be shortened by 20% to 30%. At the same time, it can also significantly improve the retention rate of tocopherols and the dissolution rate of β-glucan.

[0052] Specifically, the enzymatic hydrolysis time is 1h to 3h, the enzymatic hydrolysis temperature is 30℃ to 50℃, and the enzymatic hydrolysis pH is 4.5 to 5.5.

[0053] In a preferred embodiment, the complex enzyme is added in stages: first, pectinase is added and treated for 10 to 20 minutes; then cellulase and hemicellulase are added and treated for 20 to 115 minutes; finally, protease is added and treated for 30 to 45 minutes. Adding the enzyme in stages can maximize the release of enzyme activity and improve the enzymatic hydrolysis effect.

[0054] (2) The oat kernel enzymatic hydrolysis product is dried and pressed to extract crude oat kernel oil.

[0055] The enzymatic hydrolysate of oat kernels is dried at 80℃~90℃ to a moisture content of 8%~10%, and then pressed. The pressing includes the following steps:

[0056] (2.1) Control the pressure to 8MPa~10MPa and the temperature to 30℃~35℃ for pre-pressing. The pre-pressing time is 4min~7min.

[0057] (2.2) The pressure is controlled at 45MPa~50MPa and the temperature is controlled at 38℃~40℃ for pressing. The pressing time is 12min~20min.

[0058] (2.3) Maintain a pressure of 45MPa~50MPa and a temperature of 38℃~40℃, and perform constant pressure treatment for 3min~6min.

[0059] (2.4) Reduce the pressure to 3MPa to 5MPa.

[0060] (3) The crude oat kernel oil is degummed and deacidified to obtain the oat kernel oil.

[0061] In one embodiment, before degumming and deacidifying the crude oat kernel oil product, it is first filtered to remove impurities from the crude oat kernel oil product.

[0062] Specifically, the process begins with degumming. The crude oat kernel oil is heated to 70°C–80°C, and 0.4 wt%–0.6 wt% of soft water is added. After maintaining the temperature for 30–50 minutes, the mixture is centrifuged, and the supernatant oil phase is collected. Then, 0.15 wt%–0.3 wt% of soft water is added, and the mixture is maintained at 70°C–80°C for 30–50 minutes. After centrifugation, the degummed crude oat kernel oil is obtained.

[0063] Next, a deacidification treatment is performed. 0.4wt% to 0.6wt% sodium hydroxide is added to the crude oat oil product after degumming. After stirring for 20 to 40 minutes, the mixture is centrifuged to obtain the deacidified crude oat oil product.

[0064] Finally, the oat kernel oil crude product after deacidification is heated to 70℃~90℃, and 1wt%~2wt% of water preheated to 80℃ is added. After stirring for 4min~6min, the mixture is centrifuged. The process of adding water, stirring, and centrifuging is repeated. Then, the mixture is heated in an oil bath to 100℃~110℃ to remove residual water and obtain the oat kernel oil.

[0065] In one embodiment, the palm fruit oil is extracted using the following method:

[0066] (1) Take the fruit of the palm tree, dry it and physically crush it to obtain palm fruit powder.

[0067] The fruit of the *Phyllanthus maritima* is dried at 80℃~90℃ to a moisture content of 8%~10%, physically crushed, and sieved with a sieve mesh size of 1mm~3mm.

[0068] (2) The powder of the Mauritius fruit is subjected to supercritical extraction to separate the Mauritius fruit oil.

[0069] The supercritical extraction temperature was 40℃~50℃, the pressure was 35MPa~45MPa, the time was 40min~70min, and the CO2 flow rate was 20L / h~30L / h; the separation temperature was 30℃~40℃, and the pressure was 5MPa~10MPa.

[0070] Supercritical fluid extraction can improve the enrichment of high-value components such as glyceryl laurate in the fruit of *Malus baccata*, without leaving residues of traditional solvents and reducing the sensitization rate.

[0071] Accordingly, the present invention also provides a method for preparing the above-mentioned biomimetic skin care composition for eczema, comprising the following steps:

[0072] S10. Stir the morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, phytosterol oleate and squalane at 80℃~85℃ until completely dissolved.

[0073] In one embodiment, the oils of the fruit of the palm, oat kernel oil and jojoba seed oil are heated to 40°C to 45°C, stirred and dispersed evenly, and then phytosterol oleate and squalane are added and stirred at 80°C to 85°C until completely dissolved.

[0074] S20. Lower the temperature to 40℃~45℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on sebum film biomimicry.

[0075] In a preferred embodiment, the method for preparing the skin care composition for eczema based on sebum film biomimicry includes the following steps:

[0076] S11. Stir the mulberry fruit oil, oat kernel oil and phytosterol oleate at 80℃~85℃ until completely dissolved to obtain the first component.

[0077] S21. Jojoba seed oil, phytosterol oleate and squalane are stirred at 80℃~85℃ until completely dissolved to obtain the second component.

[0078] The mass ratio of phytosterol oleate in the first component and the second component is 1:(0.3 to 0.5), for example 1:0.32, 1:0.35, 1:0.38, 1:0.4 or 1:0.45, but not limited thereto.

[0079] S31. Mix the first component and the second component, lower the temperature to 40℃~45℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on sebum film biomimicry.

[0080] Compared to mixing murine palmetto fruit oil, oat kernel oil, and jojoba seed oil before adding phytosterol oleate and squalane, a second component is prepared by first mixing these three components to obtain the first component, and then mixing the jojoba seed oil, phytosterol oleate, and squalane. This approach maximizes the effectiveness of these three components. Under the mediation of phytosterol oleate, the compatibility of β-glucan in oat kernel oil with lauric acid in murine palmetto fruit oil is enhanced, thus strengthening the skin barrier repair ability. Under the regulation of phytosterol oleate, jojoba seed oil and squalane self-assemble into a low-viscosity microemulsion with high transdermal penetration. Finally, the first and second components are mixed, significantly improving the permeability and repair capabilities of the skincare composition.

[0081] Accordingly, the present invention also provides the application of the above-mentioned skin care composition for eczema based on sebum membrane biomimicry in the preparation of cosmetics.

[0082] In one embodiment, the cosmetic contains 5 wt% to 30 wt% of the above-mentioned biomimetic eczema skincare composition based on a sebum film, with the remainder being excipients, which are commercially available excipients conventional in the art. Preferably, the cosmetic contains 10 wt% to 15 wt% of the above-mentioned biomimetic eczema skincare composition based on a sebum film.

[0083] The present invention will be further described below with reference to specific embodiments:

[0084] Example 1

[0085] This embodiment provides a skin care composition for eczema based on biomimetic sebum film, comprising morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane, wherein the mass ratio of morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane is 1:4:1:4:3:3.

[0086] Oat kernel oil, Mauri palm fruit oil, and jojoba seed oil are all commercially available.

[0087] A method for preparing a skin care composition for eczema based on biomimetic sebum film includes the following steps:

[0088] S1. Stir the morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, phytosterol oleate and squalane at 85°C until completely dissolved.

[0089] S2. Lower the temperature to 45°C, add ceramide, and stir well to obtain a skin care composition for eczema based on the biomimetic sebum film.

[0090] Example 2

[0091] This embodiment provides a skin care composition for eczema based on biomimetic sebum film, which differs from Example 1 in that the mass ratio of morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate and squalane is 5:50:1:10:10:10.

[0092] Everything else is the same as in Example 1.

[0093] Example 3

[0094] This embodiment provides a skin care composition for eczema based on biomimetic sebum film, which differs from Example 2 in that the mass ratio of morrhagic palm fruit oil, oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate and squalane is 8:50:5:5:30:25.

[0095] Everything else is the same as in Example 2.

[0096] Example 4

[0097] This embodiment provides a skin care composition for eczema based on biomimetic sebum film, which differs from Example 3 in that the oat kernel oil is extracted using the following method:

[0098] (1) Take oat kernels at the waxy ripening stage, physically crush them, and add a compound enzyme for enzymatic hydrolysis to obtain oat kernel enzymatic hydrolysate. The compound enzyme includes cellulase, hemicellulase, pectinase and protease, with a mass ratio of 2:4:3:1. The enzymatic hydrolysis time is 2 hours, the enzymatic hydrolysis temperature is 35℃, and the enzymatic hydrolysis pH is 5.

[0099] (2) Dry the enzymatic hydrolysis product of oat kernels and press it to extract crude oat kernel oil.

[0100] (3) The crude oat kernel oil is degummed and deacidified to obtain oat kernel oil.

[0101] Everything else is the same as in Example 3.

[0102] Example 5

[0103] This embodiment provides a skin care composition for eczema based on biomimetic sebum film, which differs from Example 4 in that the oil from the palm fruit is extracted using the following method:

[0104] (1) Take the fruit of the palm tree, dry it and physically crush it to obtain palm fruit powder.

[0105] (2) The *Morris palmatum* fruit powder was subjected to supercritical extraction to obtain *Morris palmatum* fruit oil. The supercritical extraction temperature was 45℃, the pressure was 40MPa, the time was 60min, and the CO2 flow rate was 25L / h; the separation temperature was 35℃ and the pressure was 6MPa.

[0106] Everything else is the same as in Example 4.

[0107] Example 6

[0108] This embodiment provides a skin care composition for eczema based on a biomimetic sebum film. The difference between this embodiment and Example 5 is that the preparation method of the skin care composition for eczema based on a biomimetic sebum film includes the following steps:

[0109] S1. Stir the mulberry fruit oil, oat kernel oil and phytosterol oleate at 85°C until completely dissolved to obtain the first component.

[0110] S2. Jojoba seed oil, phytosterol oleate and squalane are stirred at 85°C until completely dissolved to obtain the second component.

[0111] S3. Mix the first component and the second component, lower the temperature to 45°C, add ceramide, and stir until homogeneous to obtain the skin care composition for eczema based on sebum film biomimicry.

[0112] The mass ratio of phytosterol oleate in the first component to that in the second component is 2:1.

[0113] Everything else is the same as in Example 5.

[0114] Comparative Example 1

[0115] This comparative example provides a skincare composition that differs from Example 1 in that the skincare composition includes oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane, with the mass ratio of oat kernel oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane being 4:1:4:3:3.

[0116] Everything else is the same as in Example 1.

[0117] Comparative Example 2

[0118] This comparative example provides a skincare composition that differs from Example 1 in that the plant oils include mangrove fruit oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane, with a mass ratio of mangrove fruit oil, jojoba seed oil, ceramide, phytosterol oleate, and squalane of 1:1:4:3:3.

[0119] Everything else is the same as in Example 1.

[0120] Performance testing:

[0121] The skin care compositions provided in Examples 1, 6, 1, and 2 were mixed with excipients to prepare cosmetics, wherein the mass percentage of the skin care composition was 10%, resulting in Application Examples 1 to 4.

[0122] (1) Evaluation of soothing effects

[0123] Hyaluronidase activity inhibition assay: Prepare four 10mL stoppered test tubes, named A, B, C, and D respectively. Add 0.5mL of sample solution to A and B respectively, and 0.5mL of deionized water to C and D respectively. Then add 0.5mL of hyaluronidase solution to A and C respectively, and 0.5mL of acetate buffer to B and D respectively. Place A, B, C, and D together in an oven and incubate at 37℃ for 20min. Add 0.25mmol·L⁻¹ of hyaluronidase solution to A, B, C, and D respectively. -1 After adding 0.1 mL of sodium chloride solution, place it in an oven and keep it at 37°C for 20 min; then add 0.5 mg·mL⁻¹ to A and C respectively. -12 mL of sodium hyaluronate was added to each of the following solutions: B and D. 2 mL of acetate buffer was added to each solution, and the solutions were placed in an oven at 37°C for 20 min. After incubation, the solutions were removed and allowed to stand at room temperature for 10 min. Then, 1 mL of acetylacetone solution was added to each of the following solutions. The solutions were reacted in a water bath at boiling temperature for 15 min, and then immediately placed in an ice-water bath for 15 min. After standing at room temperature for 10 min, Ehrlich chromogenic reagent was added to each of the following solutions and the mixture was shaken thoroughly. The solutions were then diluted with 3.0 mL of anhydrous ethanol and allowed to stand at room temperature for 40 min. The absorbance was measured at 520 nm using a UV-Vis spectrophotometer. The hyaluronidase activity inhibition rate was calculated using the following formula:

[0124]

[0125] In the formula, OD A OD B OD C OD D The absorbance values ​​at 520 nm are shown in the table below for the solutions in test tubes A, B, C, and D, respectively. The specific results are shown in the table below:

[0126] Hyaluronidase activity inhibition rate Example 1 83.92％ Example 2 84.27％ Example 3 84.94％ Example 4 85.13％ Example 5 86.75％ Example 6 87.14％ Comparative Example 1 61.74％ Comparative Example 2 60.05％

[0127] (2) Evaluation of repair efficacy

[0128] Thirty subjects with normal skin (15 males and 15 females, aged 18-30 years) were tested in an environment of 25±2℃ and 60% humidity. All subjects washed their arms with water and waited for 30 minutes. Five test areas were selected on the inner forearms of both subjects and marked. The initial TEWL value (T0) of the test areas was measured. Subsequently, a skin barrier damage model was established by peeling the stratum corneum of the test areas 10 times using keratolytic tape. Samples corresponding to Application Examples 1-4 were evenly applied to the test areas using latex finger cots. The test areas without any sample were designated as the control group. TEWL values ​​were measured at 2h, 4h, and 6h after application, and the TEWL change rate was calculated using the following formula:

[0129]

[0130] In the formula, T0 is the initial TEWL value of the test area, and T is the TEWL value of the test area after 2h, 4h, and 6h. The specific results are shown in the table below:

[0131]

[0132] The above description is a preferred embodiment of the invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the invention, and these improvements and modifications are also considered to be within the scope of protection of the invention.

Claims

1. A skin care composition for eczema based on biomimetic sebum film, characterized in that, The composition comprises morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil and squalane, wherein the mass ratio of morrhagic palm fruit oil, oat kernel oil, ceramide, phytosterol oleate, jojoba seed oil and squalane is (0.005-5):(0.02-20):(0.001-5):(0.001-20):(0.01-15):(0.01-15).

2. The skin care composition for eczema based on biomimetic sebum film as described in claim 1, characterized in that, The mass ratio of the following ingredients is (0.002–5):(0.02–15):(0.001–5):(0.001–15):(0.01–15):(0.01–15).

3. The skin care composition for eczema based on biomimetic sebum film as described in claim 1, characterized in that, The oat kernel oil was extracted using the following method: Wax-ripe oat kernels are physically crushed, and then enzymatically hydrolyzed with a compound enzyme to obtain oat kernel hydrolysate. The enzymatic hydrolysis product of the oat kernels was dried and pressed to extract crude oat kernel oil. The crude oat kernel oil is degummed and deacidified to obtain the oat kernel oil.

4. The skin care composition for eczema based on biomimetic sebum film as described in claim 3, characterized in that, The amount of the compound enzyme added is 1% to 4% of the dry weight of the wax-ripe oat kernels; The complex enzyme comprises cellulase, hemicellulase, pectinase, and protease; the mass ratio of cellulase, hemicellulase, pectinase, and protease is 1:(1-3):(1-2):(0.5-1); The enzymatic hydrolysis time is 1h to 3h, the enzymatic hydrolysis temperature is 30℃ to 50℃, and the enzymatic hydrolysis pH is 4.5 to 5.

5.

5. The skin care composition for eczema based on biomimetic sebum film as described in claim 1, characterized in that, The oil from the palm fruit was extracted using the following method: Take the fruit of the palm tree, dry it and physically crush it to obtain palm fruit powder; The powdered fruit of the Mauritius palm was subjected to supercritical extraction to separate and obtain the Mauritius palm oil.

6. The skin care composition for eczema based on biomimetic sebum film as described in claim 5, characterized in that, The temperature for supercritical extraction was 40℃~50℃, the pressure was 35MPa~45MPa, the time was 40min~70min, and the CO2 flow rate was 20L / h~30L / h. The separation temperature is 30℃~40℃, and the pressure is 5MPa~10MPa.

7. The skin care composition for eczema based on biomimetic sebum film as described in claim 1, characterized in that, The ceramides include one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG.

8. A method for preparing a skin care composition for eczema based on biomimetic sebum film as described in any one of claims 1 to 7, characterized in that, Includes the following steps: The fruit oil of the palm, the kernel oil of the oat, the seed oil of the jojoba, the phytosterol oleate and the squalane were stirred at 40℃~55℃ until completely dissolved. Lower the temperature to 30℃~35℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on the biomimetic sebum film.

9. The method for preparing the skin care composition for eczema based on sebum film biomimicry as described in claim 8, characterized in that, Includes the following steps: The fruit oil of the palm, the kernel oil of oats and the phytosterol oleate were stirred at 50℃~55℃ until completely dissolved to obtain the first component; Jojoba seed oil, phytosterol oleate and squalane were stirred at 40℃~45℃ until completely dissolved to obtain the second component; Mix the first component and the second component, lower the temperature to 30℃~35℃, add ceramide, and stir evenly to obtain the skin care composition for eczema based on the biomimetic sebum film. The mass ratio of phytosterol oleate in the first component and the second component is 1:(0.3-0.5).

10. The use of the biomimetic eczema skincare composition based on sebum film according to any one of claims 1 to 7 in the preparation of cosmetics, characterized in that, The cosmetic contains 5 wt% to 30 wt% of the skin care composition for eczema based on the biomimetic sebum film as described in any one of claims 1 to 7.

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