Simple device for disinfecting and cooling caenorhabditis elegans scalpel

Abstract

The invention discloses a simple device for disinfecting and cooling a caenorhabditis elegans scalpel. The simple device comprises a bottom bracket mechanism, three alcohol lamps, three overhanging supporting mechanisms and three elastic clamping mechanisms, the three alcohol lamps are respectively placed at three heating point positions on the bottom bracket mechanism and are positioned at different heights; the three overhanging supporting mechanisms are all rotationally installed on the bottom support mechanism and located at different height positions. The three elastic clamping mechanisms are fixed to the overhanging ends of the three overhanging supporting mechanisms correspondingly and used for elastically clamping the surgical blade. According to the simple device for disinfecting and cooling the scalpel, through cooperation of the three overhanging supporting mechanisms and the three elastic clamping mechanisms, stable clamping of the three scalpel blades can be achieved, manual handheld heating is not needed, and therefore the stability of heating and disinfecting of the scalpel blades is guaranteed, and the safety in the operation process can also be guaranteed.

Description

Technical Field

[0001] This invention relates to a surgical scalpel sterilization and cooling device, and more particularly to a simple device for sterilizing and cooling a surgical scalpel used for Caenorhabditis elegans. Background Technology

[0002] Caenorhabditis elegans, with its simple and transparent structure, fully defined cell fate map, short and controllable life cycle, and convenient genetic experimental procedures, has become an important model organism in the life sciences. To facilitate scientific research, wild-type Caenorhabditis elegans has been modified into a wide variety of transgenic strains (reporter gene markers, tissue-specific expression, gene overexpression, etc.) and mutant strains (point mutations, deletion mutations, insertion mutations, etc.). These abundant genetic strains provide a powerful tool foundation for life science research.

[0003] In scientific research, specific strains of *C. elegans* are typically obtained through self-construction, purchase, exchange, or donation. Almost all laboratories using *C. elegans* in their research preserve the *C. elegans* strains they have used. Furthermore, since more than one *C. elegans* strain may be used in experiments, it is necessary to culture different strains separately to avoid genetic contamination.

[0004] Dissection and transfer is a common experimental method for nematode subculturing. Specifically, *C. elegans* cultured on agar medium is dissected using a scalpel sterilized at high temperature with an alcohol lamp and cooled to room temperature. The agar block containing *C. elegans* is then transferred to a new OP50 medium containing fresh *E. coli* for further culturing. To avoid contamination between different strains of *C. elegans* and to minimize damage to the nematodes, the scalpel is usually sterilized by heating it to a bright red color on both sides with the outer flame of an alcohol lamp, then cooled to room temperature before dissection. Since the entire process of heating the scalpel to a bright red color and then cooling it to room temperature takes approximately 3-5 minutes, if the experiment or strain preservation involves the transfer of multiple strains, each dissection requires a separate sterilization of the scalpel, resulting in a long waiting time and making it difficult to stably control the scalpel blade within the outer flame of the alcohol lamp. Summary of the Invention

[0005] The purpose of this invention is to provide a simple device for sterilizing and cooling surgical blades for Caenorhabditis elegans, which can stably hold and heat the surgical blades without requiring hand-held heating, thus ensuring the stability and safety of the surgical blade heating and sterilization process.

[0006] Technical Solution: The simple device for disinfecting and cooling a scalpel for Caenorhabditis elegans according to the present invention includes a bottom support mechanism, three alcohol lamps, three suspension support mechanisms, and three elastic clamping mechanisms; the three alcohol lamps are respectively placed at three heating points on the bottom support mechanism, and the three alcohol lamps are located at different heights; the three suspension support mechanisms are all rotatably mounted on the bottom support mechanism, and the three suspension support mechanisms are located at different heights; the three elastic clamping mechanisms are respectively fixed on the suspension ends of the three suspension support mechanisms for elastically clamping the scalpel blade.

[0007] Furthermore, the bottom support mechanism includes a support base plate, a support screw, and multiple support pads; the support screw is vertically fixed at the center of the support base plate, and the three alcohol lamps are placed at three heating points respectively, and are adjusted to different heating heights by the support pads; the three cantilever support mechanisms are all rotatably mounted on the support screw.

[0008] Furthermore, the cantilever support mechanism includes a support sleeve, a rotating sleeve, a cantilever rod, and two clamping nuts; the support sleeve is movably sleeved on the support screw, and the two clamping nuts are threadedly screwed onto the support screw, respectively clamping the upper and lower ends of the support sleeve; the rotating sleeve is rotatably sleeved on the support sleeve, one end of the cantilever rod is fixed on the rotating sleeve, and the elastic clamping mechanism is installed on the other end of the cantilever rod.

[0009] Furthermore, the elastic clamping mechanism includes a blade clamping unit and an unlocking drive unit; the blade clamping unit is fixed on the cantilever end of the cantilever rod and is used to elastically clamp the surgical blade; the push unlocking unit is installed on the cantilever rod and is used to release the clamping state of the elastic clamping unit.

[0010] Furthermore, the blade clamping unit includes a clamping mounting base, two strip-shaped clamping seats, and an elastic pushing assembly; each of the two strip-shaped clamping seats has a clamping limiting strip groove on its side; the clamping mounting base is fixed to the cantilever end of the cantilever rod; the elastic pushing assembly is mounted on the clamping mounting base, and both strip-shaped clamping seats are mounted on the elastic pushing assembly. The clamping limiting strip grooves on the two strip-shaped clamping seats face each other, and the elastic pushing assembly pushes the two strip-shaped clamping seats relative to each other, so that the clamping limiting strip grooves on the two strip-shaped clamping seats elastically clamp the edge of the surgical blade.

[0011] Furthermore, the elastic pushing assembly includes two pushing springs and two supporting sliders; a sliding strip cavity is provided in the clamping mounting base, and the two pushing springs are respectively installed at both ends of the sliding strip cavity; the two supporting sliders are slidably installed in the sliding strip cavity and elastically press against the two pushing springs respectively; a strip-shaped through hole communicating with the sliding strip cavity is provided on the clamping mounting base; one end of the two strip clamping seats passes through the strip-shaped through hole and is fixed to the two supporting sliders respectively.

[0012] Furthermore, the unlocking drive unit includes an unlocking drive rod, a pushing trapezoidal block, and an unlocking rebound spring. A strip-shaped unlocking groove is provided on the wall of the sliding strip cavity, and each of the two supporting sliders has an unlocking slider that slides into the strip-shaped unlocking groove. A pushing groove is provided in the middle of the bottom of the strip-shaped unlocking groove, and the pushing trapezoidal block is movably disposed within the pushing groove to synchronously push the two unlocking sliders apart. A pull-out hole is provided at the cantilever end of the cantilever rod, and one end of the unlocking drive rod is movably installed in the pull-out hole, while the other end is movably inserted into the pushing groove and fixed to the pushing trapezoidal block. The unlocking drive rod passes through the unlocking rebound spring and has a supporting protrusion on it. The unlocking rebound spring is elastically supported on the supporting protrusion and is used to push the unlocking drive rod into the pull-out hole. A strip-shaped unlocking hole communicating with the pull-out hole is provided on the cantilever rod. A connecting post extending out of the strip-shaped unlocking hole is vertically provided on the unlocking drive rod. A pushing block is provided at the extended end of the connecting post.

[0013] Compared with the prior art, the advantages of this invention are as follows: by utilizing the cooperation of three cantilever support mechanisms and three elastic clamping mechanisms, three surgical blades can be stably clamped without the need for manual hand-held heating, thus ensuring the stability of the surgical blade heating and sterilization process and also ensuring safety during operation; by using three alcohol lamps of different heights to heat the surgical blades at different heights, the heating and sterilization of three surgical blades can be completed at one time, effectively improving operational efficiency; by setting the three cantilever support mechanisms at different heights, it is easy to rotate each cantilever support mechanism individually to clamp or release the surgical blades without obstruction or interference between them. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the front structure of the present invention;

[0015] Figure 2 This is a schematic diagram of the cantilever support mechanism of the present invention;

[0016] Figure 3 This is a cross-sectional view of the cantilever support mechanism of the present invention;

[0017] Figure 4 This is a schematic diagram of the end face structure of the elastic clamping mechanism of the present invention;

[0018] Figure 5 This is a schematic cross-sectional view of the strip clamping seat of the present invention;

[0019] Figure 6 This is a schematic diagram of the support pad structure of the present invention. Detailed Implementation

[0020] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings, but the scope of protection of the present invention is not limited to the embodiments described.

[0021] like Figure 1-6 As shown, the simple device for disinfecting and cooling a scalpel for Caenorhabditis elegans disclosed in this invention includes: a bottom support mechanism, three alcohol lamps 3, three cantilever support mechanisms, and three elastic clamping mechanisms; the three alcohol lamps 3 are respectively placed at three heating points on the bottom support mechanism, and the three alcohol lamps 3 are located at different heights; the three cantilever support mechanisms are all rotatably mounted on the bottom support mechanism, and the three cantilever support mechanisms are located at different heights; the three elastic clamping mechanisms are respectively fixed on the cantilever ends of the three cantilever support mechanisms for elastically clamping the scalpel blade 10.

[0022] By utilizing the coordination of three cantilever support mechanisms and three elastic clamping mechanisms, three surgical blades 10 can be stably clamped without the need for manual hand-held heating, thus ensuring the stability of the heating and sterilization of the surgical blades 10 and guaranteeing safety during the operation. By using three alcohol lamps 3 at different heights to heat the surgical blades 10 at different heights, the heating and sterilization of all three surgical blades 10 can be completed at once, effectively improving operational efficiency. The different height settings of the three cantilever support mechanisms allow for easy individual rotation of each cantilever support mechanism to clamp or release the surgical blades 10 without any obstruction or interference between them.

[0023] Furthermore, the three heating points are located at three of the four equally divided points of the same circle, thus leaving one free point as a clamping and releasing point, which facilitates the user's operation of the swinging cantilever support mechanism to safely perform the clamping and releasing operation of the surgical blade 10.

[0024] Furthermore, the bottom support mechanism includes a support base plate 1, a support screw 4, and multiple support pads 2; the support screw 4 is vertically fixed at the center of the support base plate 1, and the three alcohol lamps 3 are placed at three heating points respectively, and adjusted to different heating heights by the support pads 2; the three cantilever support mechanisms are all rotatably mounted on the support screw 4. The height of the alcohol lamps 3 can be adjusted as needed using the support pads 2.

[0025] Furthermore, the top of the support pad 2 is provided with a positioning hole 28, and the bottom is provided with a positioning block that cooperates with the positioning hole 28, so as to stably stack multiple support pads 2.

[0026] Furthermore, the cantilever support mechanism includes a support sleeve 5, a rotating sleeve 13, a cantilever rod 7, and two clamping nuts 6. The support sleeve 5 is movably fitted onto the support screw 4, and the two clamping nuts 6 are threadedly screwed onto the support screw 4, clamping the upper and lower ends of the support sleeve 5 respectively. The rotating sleeve 13 is rotatably fitted onto the support sleeve 5. This rotatable fitting has a certain frictional resistance, so the rotating sleeve 13 will not be easily rotated. One end of the cantilever rod 7 is fixed to the rotating sleeve 13, and the elastic clamping mechanism is installed on the other end of the cantilever rod 7. The rotatable installation of the rotating sleeve 13 and the support sleeve 5 facilitates the rotation of the cantilever rod 7 to the clamping or release point of the surgical blade 10. The cooperation of the two clamping nuts 6 with the support screw 4 enables the adjustment and locking of the height of the support sleeve 5.

[0027] Furthermore, the elastic clamping mechanism includes a blade clamping unit and an unlocking drive unit; the blade clamping unit is fixed on the cantilever end of the cantilever rod 7 and is used to elastically clamp the surgical blade 10; the push unlocking unit is installed on the cantilever rod 7 and is used to release the clamping state of the elastic clamping unit.

[0028] Furthermore, a rotating handle 14 is provided on the rotating sleeve 13 to facilitate the rotational adjustment of the cantilever rod 7.

[0029] The blade clamping unit can elastically clamp the surgical blade 10, making it easy to quickly insert the surgical blade 10 to be heated and sterilized at the clamping release point without manually adjusting the blade clamping unit; the unlocking drive unit can release the blade clamping unit, thereby releasing the surgical blade 10 from the clamping state and allowing it to fall onto the support base plate 1 for continuous cooling.

[0030] Furthermore, the blade clamping unit includes a clamping mounting base 8, two strip-shaped clamping seats 9, and an elastic pushing assembly; each of the two strip-shaped clamping seats 9 is provided with a clamping limiting strip groove 29 on its side, and a guide groove 30 is provided at the end of the clamping limiting strip groove 29; the clamping mounting base 8 is fixed to the cantilever end of the cantilever rod 7; the elastic pushing assembly is installed on the clamping mounting base 8, and both strip-shaped clamping seats 9 are installed on the elastic pushing assembly. The clamping limiting strip grooves 29 on the two strip-shaped clamping seats 9 are opposite each other, and the two guide grooves 30 are distributed in a figure-eight shape. The elastic pushing assembly pushes the two strip-shaped clamping seats 9 relative to each other, so that the clamping limiting strip grooves 29 on the two strip-shaped clamping seats 9 elastically clamp the edge of the surgical blade 10.

[0031] The surgical blade 10 can be held by the clamping and limiting grooves 29 on the two strip clamping seats 9; the two guide grooves 30 distributed in a figure-eight shape facilitate the smooth and quick insertion of the surgical blade 10.

[0032] Furthermore, the elastic pushing assembly includes two pushing springs 25 and two supporting sliders 24; a sliding strip cavity 22 is provided in the clamping mounting base 8, and the two pushing springs 25 are respectively installed at both ends of the sliding strip cavity 22; the two supporting sliders 24 are slidably installed in the sliding strip cavity 22 and elastically pressed on the two pushing springs 25 respectively; a strip-shaped through hole 23 communicating with the sliding strip cavity 22 is provided on the clamping mounting base 8; one end of the two strip-shaped clamping seats 9 passes through the strip-shaped through hole 23 and is fixed on the two supporting sliders 24 respectively.

[0033] Two push springs 25 can be used to push two support sliders 24 to move relative to each other, thereby achieving elastic clamping of the scalpel blade 10.

[0034] Furthermore, the unlocking drive unit includes an unlocking drive rod 16, a pushing trapezoidal block 20, and an unlocking rebound spring 18; a strip-shaped unlocking groove 21 is provided on the cavity wall of the sliding strip cavity 22, and an unlocking slider 26 is provided on each of the two supporting sliders 24, which is slidably embedded in the strip-shaped unlocking groove 21; a pushing groove 19 is provided in the middle of the bottom of the strip-shaped unlocking groove 21, and the pushing trapezoidal block 20 is movably disposed in the pushing groove 19 for synchronously pushing the two unlocking sliders 26 to separate; a pull hole 15 is provided at the cantilever end of the cantilever rod 7, and one end of the unlocking drive rod 16 is movably installed. The other end of the rod is inserted into the push groove 19 and fixed to the push trapezoidal block 20. The unlocking drive rod 16 passes through the unlocking return spring 18 and is provided with a support protrusion 17. The unlocking return spring 18 is elastically supported on the support protrusion 17 and is used to push the unlocking drive rod 16 into the pull hole 15. A strip-shaped unlocking hole is provided on the cantilever rod 7 and communicates with the pull hole 15. A connecting post 11 is vertically provided on the unlocking drive rod 16 and extends out of the strip-shaped unlocking hole. A push-pull block 12 is provided on the extended end of the connecting post 11.

[0035] By utilizing the unlocking drive rod 16, connecting column 11, and pushing block 12, the operator can be kept away from the heat source or high temperature of the surgical blade 10 and the elastic clamping mechanism, effectively improving the convenience and safety of use. The unlocking rebound spring 18 can elastically push the pushing trapezoidal block 20 away from the two unlocking sliders 26 after the surgical blade 10 is released from clamping and falls. Thus, under the action of the two pushing springs 25, the two strip clamping seats 9 move relative to each other, making it easy to clamp the surgical blade 10 again at the clamping release point. The two isosceles sides of the pushing trapezoidal block 20 can simultaneously push the two unlocking sliders 26 to separate, thereby separating the two strip clamping seats 9 and unlocking and dropping the surgical blade 10.

[0036] Furthermore, a guide slope 27 is provided on the unlocking slider 26 to cooperate with the waist slope of the pushing trapezoidal block 20, so that the pushing trapezoidal block 20 and the unlocking slider 26 have a relatively stable pushing contact surface.

[0037] As described above, although the invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the invention itself. Various changes in form and detail may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. A simple device for sterilizing and cooling a scalpel used for treating Caenorhabditis elegans, characterized in that: It includes a bottom support mechanism, three alcohol lamps (3), three cantilever support mechanisms and three elastic clamping mechanisms; the three alcohol lamps (3) are placed at three heating points on the bottom support mechanism and are located at different heights; the three cantilever support mechanisms are all rotatably installed on the bottom support mechanism and are located at different heights; the three elastic clamping mechanisms are fixed on the cantilever ends of the three cantilever support mechanisms and are used to elastically clamp the scalpel blade (10).

2. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 1, characterized in that: The bottom support mechanism includes a support base plate (1), a support screw (4) and multiple support pads (2); the support screw (4) is vertically fixed at the center of the support base plate (1), and three alcohol lamps (3) are placed at three heating points respectively, and are adjusted to different heating heights by the support pads (2); the three cantilever support mechanisms are all rotatably installed on the support screw (4).

3. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 2, characterized in that: The cantilever support mechanism includes a support sleeve (5), a rotating sleeve (13), a cantilever rod (7), and two clamping nuts (6); the support sleeve (5) is movably sleeved on the support screw (4), and the two clamping nuts (6) are threadedly screwed onto the support screw (4) and clamp the upper and lower ends of the support sleeve (5) respectively; the rotating sleeve (13) is rotatably sleeved on the support sleeve (5), one end of the cantilever rod (7) is fixed on the rotating sleeve (13), and the elastic clamping mechanism is installed on the other end of the cantilever rod (7).

4. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 3, characterized in that: The elastic clamping mechanism includes a blade clamping unit and an unlocking drive unit; the blade clamping unit is fixed on the cantilever end of the cantilever rod (7) and is used to elastically clamp the surgical blade (10); the push unlocking unit is installed on the cantilever rod (7) and is used to release the clamping state of the elastic clamping unit.

5. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 4, characterized in that: The blade clamping unit includes a clamping mounting base (8), two strip clamping seats (9), and an elastic pushing assembly; a clamping limiting strip groove (29) is provided on the side of each of the two strip clamping seats (9); the clamping mounting base (8) is fixed on the cantilever end of the cantilever rod (7); the elastic pushing assembly is installed on the clamping mounting base (8), and the two strip clamping seats (9) are both installed on the elastic pushing assembly. The clamping limiting strip grooves (29) on the two strip clamping seats (9) are opposite to each other. The elastic pushing assembly pushes the two strip clamping seats (9) relative to each other, so that the clamping limiting strip grooves (29) on the two strip clamping seats (9) elastically clamp the edge of the surgical blade (10).

6. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 5, characterized in that: The elastic push assembly includes two push springs (25) and two support sliders (24); a sliding strip cavity (22) is provided in the clamping mounting base (8), and the two push springs (25) are respectively installed at both ends of the sliding strip cavity (22); the two support sliders (24) are slidably installed in the sliding strip cavity (22) and are elastically pressed on the two push springs (25) respectively; a strip-shaped through hole (23) communicating with the sliding strip cavity (22) is provided on the clamping mounting base (8); one end of the two strip clamping seats (9) passes through the strip-shaped through hole (23) and is fixed on the two support sliders (24) respectively.

7. The simple device for sterilizing and cooling a scalpel for nematode surgery according to claim 6, characterized in that: The unlocking drive unit includes an unlocking drive rod (16), a pushing trapezoidal block (20), and an unlocking rebound spring (18). A strip-shaped unlocking groove (21) is provided on the cavity wall of the sliding strip cavity (22), and an unlocking slider (26) is provided on each of the two support sliders (24) and is slidably embedded in the strip-shaped unlocking groove (21). A pushing groove (19) is provided in the middle of the bottom of the strip-shaped unlocking groove (21), and the pushing trapezoidal block (20) is movably disposed in the pushing groove (19) to synchronously push the two unlocking sliders (26) to separate. A pull-out hole (15) is provided at the cantilever end of the cantilever rod (7), and one end of the unlocking drive rod (16) is movably installed in the pull-out hole. The other end is inserted into the push groove (19) and fixed on the push trapezoidal block (20); the unlocking drive rod (16) passes through the unlocking rebound spring (18) and a support convex ring (17) is provided on the unlocking drive rod (16); the unlocking rebound spring (18) is elastically supported on the support convex ring (17) and is used to push the unlocking drive rod (16) to move into the pull hole (15); a strip-shaped unlocking hole connected to the pull hole (15) is provided on the cantilever rod (7); a connecting post (11) extending out of the strip-shaped unlocking hole is vertically provided on the unlocking drive rod (16); a push-pull block (12) is provided on the extended end of the connecting post (11).

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