Foliage spraying method for promoting growth of regenerated buds of ratooning rice and application

Abstract

The invention provides a leaf surface spraying method for promoting growth of regenerated buds of ratooning rice and application, and belongs to the technical field of ratooning rice cultivation. According to the method, 10-20 days after full heading of the ratooning rice in the first season, the 2, 4-epibrassinolide solution with the concentration being 0.3-0.5 mg / L is sprayed to leaf surfaces, growth and development of regeneration buds are promoted, and a good foundation is laid for high yield of the ratooning rice in the regeneration season.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of regenerative rice cultivation, and particularly relates to a foliar spraying method for promoting the growth of regenerative buds of regenerative rice and application. BACKGROUND

[0002] Regenerative rice refers to a planting mode in which the dormant buds (regenerative buds) on the rice stubble after the harvest of the first season of rice grow into ears and a second season of rice is harvested. The mode has advantages such as two harvests, labor and seed saving, yield and efficiency increasing, and fertilizer and pesticide reducing. The rapid development of regenerative rice highlights the regional advantage of "one season with surplus and two seasons with deficiency", and meets the characteristics of modern agricultural green production. Especially under the condition that the arable land area cannot be greatly increased and the yield of crops increases slowly, regenerative rice has become an important measure to improve the total yield of rice and ensure food security.

[0003] At present, the yield of the regenerative season of regenerative rice is 30% to 65% of the yield of the first season, only about 53% of the yield potential of the regenerative season, and there is still a large room for improvement. At the same time, the yield of the regenerative season varies greatly between years and regions, which seriously hinders the further promotion and application of regenerative rice. The formation of the yield of the regenerative season mainly depends on the germination of regenerative buds and the quality of seedlings, therefore, promoting the healthy growth of regenerative buds is a key measure to seize high yield in the regenerative season. SUMMARY

[0004] Therefore, the present application aims to provide a foliar spraying method for promoting the growth of regenerative buds of regenerative rice and application.

[0005] In order to achieve the above-mentioned application purpose, the present application provides the following technical solutions: The present application provides a foliar spraying method for promoting the growth of regenerative buds of regenerative rice, comprising the following steps: Spraying a 2,4-epibrassinolide solution with a concentration of 0.3 to 0.5 mg / L 10 to 20 days after the heading of the first season of rice; The spraying amount of the 2,4-epibrassinolide solution is 500 to 1000 kg / hm 2 .

[0006] Preferably, the spraying position of the 2,4-epibrassinolide solution includes the third node from the bottom and / or the fourth node from the bottom.

[0007] Preferably, the spraying frequency of the 2,4-epibrassinolide solution is 1 to 5 times; The interval between spraying is 12 to 36 h.

[0008] Preferably, promoting the growth of regenerative buds of regenerative rice includes increasing the number of regenerative buds and / or increasing the dry weight of regenerative buds.

[0009] The present application provides the application of the foliar spraying method in improving the yield of regenerative rice.

[0010] Compared with the prior art, the application has the following beneficial effects: The application provides a leaf surface spraying method for promoting the growth of regenerative rice regenerative buds and application. The application sprays a 2,4-epibrassinolide solution with a concentration of 0.3-0.5 mg / L to the leaf surface 10-20 days after the heading of the first season of rice, promotes the growth and development of regenerative buds, and lays a good foundation for high yield in the regenerative season of rice. BRIEF DESCRIPTION OF DRAWINGS

[0011] Figure 1 Regenerative force of the regenerative rice under the treatment of different concentrations of 2,4-epibrassinolide. DETAILED DESCRIPTION

[0012] The application provides a leaf surface spraying method for promoting the growth of regenerative rice regenerative buds, comprising the following steps: Spraying a 2,4-epibrassinolide solution with a concentration of 0.3-0.5 mg / L 10-20 days after the heading of the first season of rice; In the application, the concentration of the sprayed 2,4-epibrassinolide solution is 0.3-0.5 mg / L, preferably 0.35-0.45 mg / L, and further preferably 0.4 mg / L; the spraying time is 10-20 days after the heading of the first season of rice, preferably 12-18 days, and further preferably 15 days.

[0013] In the application, the spraying position of the 2,4-epibrassinolide solution preferably includes the third node from the bottom and / or the fourth node from the bottom, and further preferably the fourth node from the bottom; the node position of rice is the position and order of the nodes on the stem of rice, and the node position of rice is the “hinge” connecting the root system, the leaf, the tiller and the ear, and through understanding and regulating the growth and development of different node positions, agricultural technicians and rice growers can more accurately carry out fertilizer and water management, disease and pest control and population regulation, and finally achieve the goal of high yield, high quality and high efficiency; the panicle neck node of rice is the node directly attached to the ear, which is the first node at the top of the main stem or tiller stem and is also called “the first node from the bottom”, and the nodes below the panicle neck node are “the second node from the bottom”, “the third node from the bottom”, “the fourth node from the bottom” and the like.

[0014] In the application, the spraying amount of the 2,4-epibrassinolide solution is 500-1000 kg / hm 2 , preferably 600-900 kg / hm 2 , and further preferably 750 kg / hm 2 ; the spraying frequency is preferably 1-5 times, further preferably 2-4 times, and more further preferably 3 times, and the interval of each spraying is preferably 12-36 h, further preferably 18-30 h, and more further preferably 24 h.

[0015] In the present application, the 2,4-epibrassinolide solution is preferably mixed with Tween 20, and the volume of Tween 20 used is preferably 0.001% to 0.02%, further preferably 0.005% to 0.015%, and more preferably 0.01% of the volume of the 2,4-epibrassinolide solution.

[0016] In the present application, promoting the growth of the tillers of the ratooning rice includes increasing the number of tillers and / or increasing the dry weight of the tillers.

[0017] The present application provides the use of the foliar spraying method in increasing the yield of the ratooning rice.

[0018] The technical solutions provided by the present application will be described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present application.

[0019] Example 1

[0020] Effects of different concentrations of 2,4-epibrassinolide

[0021] At the grain-filling stage of the ratooning rice (15 days after heading), on the basis of normal application of the ratooning rice tiller-promoting fertilizer (urea 10 kg / mu, and the application time is 15 days after the heading of the first season), 2,4-epibrassinolide is sprayed on the leaves at 16:00-19:00 on sunny and windless days, the concentration of 2,4-epibrassinolide (C 28 H 48 O6) is adjusted to 0.4 mg / L and 0.8 mg / L, 750 kg per mu is sprayed, and 0.01% (v / v) of Tween 20 is added before spraying for uniform spraying. The control group is sprayed with the same dose of water. The time for statistical results is the harvesting period of the first season. The results are shown in Table 1 and Figure 1 .

[0022] As shown in Table 1, at the grain-filling stage of the ratooning rice, spraying 2,4-epibrassinolide with a suitable concentration is beneficial to increasing the length of the tillers.

[0023] In terms of the number of tillers, the number of the third and fourth nodes of the tillers under the treatment of spraying 0.8 mg / L 2,4-epibrassinolide is the largest, which is 227 / m 2 and 246 / m 2 , respectively, which is increased by 24.7% and 28.8% compared with the control, respectively. However, compared with the treatment of spraying 0.4 mg / L 2,4-epibrassinolide, the number of the third and fourth nodes of the tillers has no significant difference. In addition, the number of the third and fourth nodes of the tillers under the treatment of spraying 0.4 mg / L 2,4-epibrassinolide is higher than that of the control, but the difference is not significant.

[0024] Regarding the length of regenerated shoots per plant, the treatment with 0.4 mg / L 2,4-epibrassinolide resulted in the longest regenerated shoots at the third and fourth nodes from the bottom, measuring 7.7 cm and 11.0 cm respectively, representing increases of 30.5% and 26.4% compared to the control. Furthermore, the lengths of regenerated shoots at the third and fourth nodes from the bottom under the 0.4 mg / L 2,4-epibrassinolide treatment were significantly higher than those under the 0.8 mg / L treatment, increasing by 42.2% and 66.7% respectively. However, the length of regenerated shoots at the third node from the bottom under the 0.8 mg / L 2,4-epibrassinolide treatment was not significantly different from the control, but the length of regenerated shoots at the fourth node from the bottom was significantly shorter than the control.

[0025] Regarding the dry weight of regenerated shoots per plant, the dry weight of shoots from the fourth node from the bottom was greater than that from the third node in all treatments. Simultaneously, the dry weight of shoots per plant treated with 0.4 mg / L 2,4-epibrassinolide was significantly higher than other treatments, with the fourth node from the bottom showing a significant difference. Compared to the control, the dry weight of the third and fourth nodes from the bottom increased by 10.9% and 71.9%, respectively, under the 0.4 mg / L 2,4-epibrassinolide treatment. The dry weight of the third and fourth nodes from the bottom under the 0.4 mg / L 2,4-epibrassinolide treatment did not differ significantly from the control.

[0026] Therefore, spraying with 0.4 mg / L 2,4-epibrassinol is beneficial for promoting the growth of regenerated shoots, while excessively high concentrations (0.8 mg / L) may inhibit the growth of regenerated shoots.

[0027] Table 1. Growth of regenerated buds at different nodes under each treatment.

[0028] Depend on Figure 1 It was found that spraying an appropriate concentration of 2,4-epibrassinolide during the grain-filling stage of the first harvest of ratooning rice was beneficial to improving the regeneration capacity of ratooning rice. The highest regeneration capacity (1.93) was observed under the 0.4 mg / L 2,4-epibrassinolide treatment, which was 20.6% higher than the control treatment (sprayed with water). Furthermore, there was no significant difference in the regeneration capacity between the 0.4 mg / L and 0.8 mg / L 2,4-epibrassinolide treatments, nor was there a significant difference between the 0.8 mg / L and control treatments (sprayed with water). Regeneration capacity refers to the ability of dormant buds surviving on the rice stubble after the first harvest to germinate, grow, and generate economic yield again (in the ratoon season). The formula for calculating regeneration capacity is: Regeneration capacity = Effective panicles in the ratoon season / Effective panicles in the first harvest.

[0029] Example 2

[0030] Comparison of the effects of foliar spraying of 2,4-epibrassinolone at different time periods

[0031] A 0.4 mg / L solution of 2,4-epibrassinolide was sprayed at the heading stage of the first crop, 15 days after the heading stage, and 1 day after the harvest of the first crop. The results are shown in Table 2.

[0032] Table 2 shows that foliar spraying of 2,4-epibrassinolide at different time points significantly affected the growth of regenerated shoots in ratooning rice. Regarding the length of individual regenerated shoots, spraying 2,4-epibrassinolide 15 days after the first heading stage was beneficial in increasing the length of regenerated shoots. Compared with the treatments of spraying 2,4-epibrassinolide at the first heading stage and 1 day after the first harvest, the third-to-last node length increased by 21.2% and 35.6% respectively, and the fourth-to-last node length increased by 37.6% and 39.3% respectively. Furthermore, there was no significant difference in the length of regenerated shoots at the third-to-last and fourth-to-last nodes under the treatments of spraying 2,4-epibrassinolide at the first heading stage and 1 day after the first harvest. Regarding the number of regenerated shoots, the number of the third-to-last and fourth-to-last nodes was highest under the treatment of spraying 2,4-epibrassinolide 15 days after the first heading stage, reaching 264 shoots / m². 2 and 255 / m 2 Compared to the first heading stage, the number of shoots increased by 38.2% and 47.4%, respectively. Furthermore, there was no significant difference in the number of regenerated shoots at the third and fourth nodes from the bottom under the 2,4-epibrassin lactone treatment at the first heading stage and one day after the first harvest.

[0033] Table 2. Growth of regenerated buds at different nodes during different spraying periods.

[0034] Example 3

[0035] The effects of spraying a 0.4 mg / L 2,4-epibrassinolide solution three times, 15 days after the first heading stage, were compared with spraying 2,4-epibrassinolide once each at the first heading stage, 15 days after the first heading stage, and one day after the first harvest. The results are shown in Table 3. The three applications of the 0.4 mg / L 2,4-epibrassinolide solution were applied consecutively for three days, once a day.

[0036] Table 3 shows that spraying 2,4-epibrassinolide three times consecutively 15 days after the first heading stage has a significantly different effect compared to spraying it once each at the first heading stage, 15 days after the first heading stage, and 1 day after the first harvest. Regarding the length of regenerated shoots per plant, spraying 2,4-epibrassinolide three times 15 days after the first heading stage is beneficial in increasing the length of regenerated shoots. Compared to the treatments of spraying 2,4-epibrassinolide once each at the first heading stage, spraying it once at the first heading stage, and spraying it once at the first harvest, the third and fourth nodes from the bottom increased by 30.2% and 31.0%, respectively, in the treatment 15 days after the first heading stage. Regarding the number of regenerated shoots, the treatment of spraying 2,4-epibrassinolide three times 15 days after the first heading stage had the highest number of third and fourth nodes from the bottom, at 260 shoots / m². 2 and 256 / m 2 Compared with the treatments of spraying 2,4-epibrassinolone at the first heading stage, 15 days after the first heading stage, and 1 day after the first harvest (each once), the benefits increased by 43.6% and 44.7%, respectively.

[0037] Table 3. Growth of regenerated buds at different nodes during different spraying periods.

[0038] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A foliar spraying method for promoting the growth of tillers of regenerated rice, characterized in that, Comprising the following steps: Spraying 2,4-epibrassinolide solution with a concentration of 0.3-0.5 mg / L at 10-20 days after the heading of the first season of rice; The spraying amount of the 2,4-epibrassinolide solution is 500-1000 kg / hm 2 .

2. The foliage spraying method according to claim 1, characterized by, The spraying position of the 2,4-epibrassinolide solution includes the third and / or fourth nodes from the top.

3. The foliage spraying method according to claim 1, characterized by, The spraying frequency of the 2,4-epibrassinolide solution is 1-5 times. The interval between sprays is 12-36 h.

4. The foliage spraying method according to claim 1, characterized by Promoting the growth of the tillers of the ratooning rice includes increasing the number of tillers and / or increasing the dry weight of the tillers.

5. Application of the foliar spraying method of any one of claims 1-4 in increasing the yield of ratooning rice.

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