Intensive artificial breeding method of procambarus clarkii

Abstract

A method for intensive artificial breeding of *Procambarus clarkii* (red swamp crayfish) relates to the field of aquaculture technology. This intensive breeding device includes broodstock nesting and mating structures and berried crayfish hatching and larval rearing structures, respectively installed in broodstock rearing ponds and hatching and larval rearing ponds. The broodstock nesting and mating structures include a perch frame and an inner nest. The perch frame is a right-angled trapezoidal frame with multiple partitions in the middle. The inner nest is also a right-angled trapezoidal body with multiple perch burrows inside. The berried crayfish hatching and larval rearing structures include a hatching and rearing frame, hatching nest units, and side nets. This device effectively improves the space utilization rate of broodstock and larvae in the broodstock rearing ponds and hatching and larval rearing ponds, significantly increasing the carrying capacity and stocking density of broodstock and larvae per unit water volume. This invention also discloses an intensive artificial breeding method, realizing the intensive and large-scale production of berried crayfish and larvae of *Procambarus clarkii*, increasing production capacity by more than five times.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, and in particular to an intensive artificial breeding method for red swamp crayfish. Background Technology

[0002] The red swamp crayfish, also known as the crayfish, originated in the central and western regions of North America and was introduced to my country in the 1930s. It has now become an important freshwater economic species in my country, with a total aquaculture volume exceeding 3 million tons nationwide. However, the current production of red swamp crayfish seedlings still relies mainly on self-breeding in rice paddies and ponds. Insufficient seedling breeding technology and capacity is one of the main bottlenecks restricting the sustainable development of my country's red swamp crayfish aquaculture industry.

[0003] In recent years, both those engaged in red swamp crayfish (Procambarus clarkii) farming and researchers in related institutions have recognized that intensive artificial breeding of red swamp crayfish is key to fundamentally solving the problem of self-breeding and self-raising. Significant progress has been made, primarily in pond-based large-scale breeding systems, indoor factory-style breeding systems, and in vitro incubation technology. Pond-based large-scale breeding systems and indoor factory-style breeding systems have addressed the issue of uncontrollable seedling production under rice paddy or pond self-breeding conditions. However, two major limitations to intensive artificial breeding of red swamp crayfish remain: ① Low absolute egg-bearing capacity and difficulty in determining gonadal development stages, resulting in low economic value for single female crayfish and difficulty in large-scale stockpiling of synchronously developing broodstock; ② Red swamp crayfish belong to the suborder Pleurocystis and are benthic reptiles exhibiting fighting behavior, leading to low space utilization for both broodstock and larvae. These problems have not yet been effectively resolved.

[0004] Research on improving the space utilization of broodstock crayfish has largely focused on multi-layered nest structures and concealment structures. For example, CN 223437676 U discloses an artificial breeding nest structure for crayfish farming in sandy soil in arid Northwest China. This structure comprises multiple stacked breeding devices, each including a breeding tube and a breeding net. The breeding tube has openings at both ends for crayfish to enter and exit, and has strip-shaped through holes and multiple flow holes along its length. At least two breeding tubes are arranged opposite each other, and the two ends of the breeding net pass through the strip-shaped through holes of two of the breeding tubes. The breeding net and the breeding tubes are fixed together with PP cable ties. This theoretically solves the problem of crayfish difficulty climbing other multi-layered nest structures, but its stepped nest units only provide habitat in the water and do not effectively separate living and living spaces. Secondly, in aquaculture water bodies, when nesting units are distributed in a gradient design, it is difficult for the red swamp crayfish, which is benthic and horizontally distributed, to be layered within the gradient-designed nesting units according to human intention. Its distribution mode is still mainly bottom-dwelling. Therefore, this method is unlikely to achieve the expected results in practical applications.

[0005] From a theoretical perspective, in vitro incubation technology can solve two limiting problems in the intensive artificial breeding of red swamp crayfish. However, the low hatching rate and survival rate of red swamp crayfish under in vitro conditions have always limited the practical application of this technology.

[0006] Therefore, there is an urgent need to propose effective technical solutions to address the technical problems that limit the intensive artificial breeding of Procambarus clarkii, thereby changing the problem of low space utilization in the rearing of broodstock and larvae of Procambarus clarkii. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a method for intensive artificial breeding of red swamp crayfish using the intensive breeding device.

[0008] In one exemplary embodiment, the technical solution adopted by the present invention to solve its technical problem is as follows: An intensive breeding device for Procambarus clarkii includes a nest structure for broodstock to inhabit and mate, and a nest structure for berried shrimp to hatch and raise larvae. The broodstock to inhabit and mate nest structure and the berried shrimp to hatch and raise larvae nest structure are respectively set in the broodstock breeding pond and the hatching and raising pond. The nest structure for the parent shrimp to inhabit and mate includes a habitat frame and an inner nest. The habitat frame is a right-angled trapezoidal frame with multiple partitions in the middle, dividing the habitat frame into multiple habitat spaces distributed in a stepped manner. The inner nest is also a right-angled trapezoidal body with multiple habitat burrows extending through its front and back. The size of the inner nest is adapted to the habitat space placed between the habitat frame and the partitions. The hatching and larval rearing nest structure for berried shrimp includes a hatching and rearing frame, hatching nest units, and side nets. The hatching and rearing frame includes a skeleton and crossbeams, with the crossbeams overlapping the skeleton. The shape of the hatching nest unit is adapted to the hatching and rearing frame and is assembled from plastic mesh sheets, with a through-hole hatching and rearing nest in the middle. The side nets are placed on the front and rear sides of the hatching and rearing nest to close the front and rear ends of the hatching and rearing nest.

[0009] The intensive breeding device for Procambarus clarkii used in this invention utilizes a right-angled trapezoidal habitat frame and inner nest to divide the breeding pond into multiple stepped habitat spaces. The space extending from the hypotenuse of the right-angled trapezoid can form a stepped living space for receiving food (feed is thrown from above the breeding pond, and the feed sinks under gravity. When it hits the bottom plate of the inner nest corresponding to the hypotenuse of the right-angled trapezoid, it stops sinking and is then dispersed on the inner nests of each layer). This solves the feeding needs of the benthic Procambarus clarkii, eliminating the existing habit in which Procambarus clarkii, although able to live in a stepped habitat, still needs to enter the bottom of the breeding pond to feed. This device truly separates the living and habitat spaces of the broodstock, effectively improving the space utilization rate of the broodstock in the breeding pond and significantly increasing the number of broodstock carried per unit of water.

[0010] Secondly, its nest structure for hatching and raising larvae can separate parent shrimp and berried shrimp for separate rearing. The independent hatching nest unit facilitates the separation of individual berried shrimp and their phased rearing, thereby controlling the difference in the spawning period of berried shrimp within one week. This allows fertilized eggs at the same stage to be raised and hatched under the same hatching conditions, further reducing the rearing differences between different batches of red swamp crayfish larvae and achieving consistency in the size of the same batch of red swamp crayfish seedlings.

[0011] The skeleton is a cuboid assembled from PVC pipes.

[0012] In one exemplary embodiment, the upper base of the right-angled trapezoidal frame is 15-20cm long, the lower base is 35-40cm long, the height is 25-30cm, and the distance between adjacent partitions is 5-6cm. The entrance to the nest is 6-8cm long and 5-6cm wide.

[0013] The incubation and cultivation frame is 35-40cm long, 13-15cm wide, and 35-40cm high; the plastic mesh has a mesh size of 0.1-0.2cm; and the opening of the incubation and cultivation nest is 5-7cm long and 4-6cm wide.

[0014] The broodstock shrimp rearing pond includes a long trough-shaped pool, an inlet / outlet device, and an aeration device. Two mesh screens are placed in the center of the long trough-shaped pool, with the sides of the mesh screens abutting against the pool and a distance of 3-5 cm between the two mesh screens. The inlet / outlet device includes an inlet pipe, an inlet, a drain pipe, and an outlet. The inlet pipe is arranged along the edge of the long trough-shaped pool, and the drain pipe is buried at the bottom of the pool. The inlet and outlet are connected to the inlet and outlet pipes, respectively, with the inlet being higher than the outlet. The aeration device is arranged parallel to the bottom, sides, and intervals of the mesh screens of the long trough-shaped pool.

[0015] An oxygenation device is installed in the broodstock rearing pond to effectively provide dissolved oxygen and ensure high dissolved oxygen content in the water. This meets the high dissolved oxygen requirements for gonad development in high-density cultured Procambarus clarkii, thereby achieving the goal of high-density intensive seedling cultivation.

[0016] The oxygenation device includes at least three nano-oxygenation pipes.

[0017] The long, trough-shaped water tank is equipped with a shade net, which has a shading rate of 60-70%.

[0018] The hatching and larval rearing pool includes a hatching pool, an inlet and outlet drainage device, and an oxygenation device. The inlet and outlet drainage device includes an inlet pipe, an inlet, a outlet pipe, and an outlet. The inlet pipe is arranged along the edge of the hatching pool, and the outlet pipe is buried at the bottom of the hatching pool. The inlet and outlet are connected to the inlet pipe and the outlet pipe, respectively, and the inlet is higher than the outlet. The oxygenation device includes several nano-oxygenation pipes laid parallel to each other at the bottom of the hatching pool.

[0019] The spacing between adjacent nano-oxygenation pipes is 15~20cm.

[0020] In one exemplary embodiment, the trough-shaped pool is 80-100cm wide, 80cm high, and 3-15m long; the mesh size of the partition net is 0.8-1.5cm; the inlet and outlet are spaced 1-1.5m apart; and the hatching pool is 100cm wide, 80cm high, and 3-15m long.

[0021] High-density arrangement of nano-oxygenation pipes in the hatching pond significantly improved the oxygen uptake difficulty for juvenile red swamp crayfish and increased their survival rate.

[0022] A method for intensive artificial breeding of red swamp crayfish, comprising the aforementioned intensive breeding device for red swamp crayfish, and including the following steps: S1. Preparation and pretreatment of intensive breeding equipment for red swamp crayfish; Before the broodstock shrimp are placed in the broodstock rearing pond, a habitat and mating nest structure for the broodstock shrimp is arranged in the broodstock rearing pond. The back of the habitat and mating nest structure is connected to the middle partition net of the broodstock rearing pond. A shade net (with a shading rate of 60-70%) is set up above the broodstock rearing pond. One week before the collection and transfer of berried shrimp, berried shrimp hatching and larval rearing nest structures are arranged in the hatching pond. The berried shrimp hatching and larval rearing nest structures are placed in parallel in the hatching pond, with an interval of 3-5 cm between adjacent berried shrimp hatching and larval rearing nest structures. The prepared broodstock breeding ponds and hatching ponds are cleaned and disinfected before pretreatment. S2. Daily Management of Broodstock Shrimp Cultivation: The water level in the broodstock shrimp rearing pond should be maintained at 30-40cm, and oxygenation should be increased until the water surface is slightly undulating. The same batch of broodstock shrimp whose gonads have developed to stage II (pale yellow) were placed in the broodstock rearing pond for gonad enhancement rearing; the size of the broodstock shrimp was 18g-25g, and their appendages were intact and undamaged; the number of broodstock shrimp was 100-150 per square meter of water surface, and the female-to-male ratio was controlled at 2-2.5:1. The broodstock of *Procambarus clarkii* were fed once daily with a formulated feed containing 36% crude protein. The daily feeding amount was 2 ± 0.5% of the broodstock's body weight, and the feeding time was from 4:00 PM to 6:00 PM. In addition to the daily feeding of the formulated feed, every 3 days, fermented feed and fresh feed were fed once each. On the 4th day, fermented feed using lactic acid bacteria as the fermentation starter was fed once, at the same amount as the formulated feed. On the 5th day, fresh feed was fed once, at 5% of the broodstock's body weight. Regularly check the gonadal development of broodstock shrimp, once every 15 days in the early stage and once every 5 days in the later stage, to observe whether the gonads have developed to stage V as a whole; when the number of broodstock shrimp in the breeding pond that have developed to stage V reaches more than 80%, the broodstock shrimp in the breeding pond are subjected to pre-spawning environmental stimulation by the dry pond transfer method. Water should be changed every five days. Aeration should be turned off ten minutes before water change. Bottom drainage should be used for water change, and each water change should replace about one-fifth of the total water volume. After water change, activated Bacillus subtilis should be sprinkled throughout the pond. During the broodstock shrimp rearing period, the water quality conditions are as follows: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, and nitrite ≤0.05mg / L.

[0023] S3. Daily management of berried shrimp hatching: Based on the embryonic development stage of the broodstock shrimp in the breeding pond, the broodstock shrimp were collected in batches and transferred to the hatching pond (15) for further cultivation; Check the berried shrimp and embryo development in the hatching pond (15) every two days. If any berried shrimp die or other diseases occur, isolate and treat them in time. The water level in the hatching pond should be maintained at 40cm. Activated Bacillus and Oocystis should be sprayed throughout the pond. Ammonium chloride should be added at a dosage of 4ppm, potassium dihydrogen phosphate at a dosage of 1ppm, and molasses at a dosage of 60ppm. Aeration should be increased until the water surface is slightly boiling. The relevant physicochemical indicators of the water should be monitored daily, and small amounts of ammonium chloride, molasses, phosphate fertilizer, and trace elements should be added as needed. The water quality conditions should be maintained as follows: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water not lower than 100mg / L.

[0024] S4. Daily Management of Larval Fry: During the later stages of hatching of the red swamp crayfish, the feeding management during the larval rearing process is as follows: when about 40% of the larvae have hatched, use a 100-mesh net bag to grind and dissolve the special compound feed for crayfish larvae (38% crude protein content) in water and then sprinkle it throughout the pond at a dosage of 2 ppm. At the same time, molasses should be added at a dosage of 2 ppm once a day. Once all larvae have emerged from the membrane and 10% have shown signs of separation, mix shrimp larvae-specific formulated feed (38% crude protein content) and yeast-based starter feed in a 3:2 ratio. Use 4 ppm of the mixed feed, grind it in a 100-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 4 ppm. Repeat this process once a day until all larvae have shown signs of separation. Then, mix shrimp larvae-specific formulated feed (38% crude protein content), black shrimp flakes, and yeast-based starter feed in a 4:3:3 ratio. Use 5 ppm of the mixed feed, grind it in an 80-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 5 ppm. Repeat this process once a day until all larvae have separated from the membrane and have grown to juvenile shrimp (0.8-1 cm in length).

[0025] During the juvenile rearing period, relevant physicochemical indicators of the water body are monitored daily. Small amounts of ammonium chloride, phosphate fertilizer, and trace elements are added as needed. Activated Bacillus and Oocystis are added regularly to ensure the following water quality conditions: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water body not lower than 100mg / L.

[0026] In step S1, the disinfection operation is as follows: after cleaning, add water to the broodstock breeding pond and hatching pond to a water level of 40cm, and sprinkle trichloroisocyanuric acid at a dosage of 0.5-1ppm (based on available chlorine). Let it stand and soak for 24 hours. After standing and soaking, turn on the aeration device until the water surface is boiling, and fully aerate for 24 hours until the residual chlorine content is lower than 0.1ppm.

[0027] In step S2, before placing the broodstock shrimp into the broodstock rearing pond, they are soaked in a 50 ppm chlorine dioxide solution for 5 minutes. The broodstock shrimp in the same batch refer to a population of Procambarus clarkii cultivated in the same production environment from the larval rearing period, the growth stage to the later rearing stage, and the spawning cycle of this batch of broodstock shrimp is completed within one week.

[0028] The specific operation of the dry pond transfer method is as follows: First, drain the broodstock rearing pond, then collect and select the broodstock, keeping only the females, and transfer the selected females to another broodstock rearing pond. Each batch of broodstock should be transferred within 3-5 hours. After the dry pond transfer, the broodstock should be temporarily kept in the same daily broodstock rearing management methods for one week. In step S3, pre-spawning environmental stimulation is performed using the dry pond transfer method before transfer. After the broodstock shrimp are temporarily held, the normalized collection and transfer of berried shrimp begins. The specific operation of collecting and transferring berried shrimp in batches is as follows: After one week of temporary holding, the broodstock shrimp undergo normalized collection, that is, berried shrimp are collected every 7 days. The collected berried shrimp are transferred to the hatching pond in batches according to the embryonic development stage of the fertilized eggs. The berried shrimp are placed in the hatching and rearing nests, with only one broodstock shrimp in each hatching and rearing nest. After the berried shrimp are placed, the openings on both sides of the hatching and rearing nests are sealed with side nets, and the berried shrimp hatching and larval rearing nest structure is placed in the hatching pond.

[0029] The collected berried shrimp were transferred to the hatching pond in batches according to the embryonic development stage of the fertilized eggs. Specifically, the berried shrimp were transferred in batches according to the three different stages of embryonic development: pre-gastrulus stage, nauplius stage, and pre-embryonic stage. It is preferable to transfer them to the hatching pond in 3 batches, that is, the berried shrimp whose embryonic development is in the pre-gastrulus stage, nauplius stage, and pre-embryonic stage are transferred in 3 batches.

[0030] This invention, by transferring berried shrimp to the hatching pond in batches, can control the difference in the spawning period of the produced red swamp crayfish to within one week, ensuring higher consistency in the size of the seedlings in the later batches. This is beneficial for the selection and cultivation of subsequent broodstock shrimp, thereby increasing the farming capacity of red swamp crayfish.

[0031] The daily feeding amount of the broodstock feed is based on 2% of the broodstock's body weight, and is adjusted daily according to the amount of uneaten feed the following morning.

[0032] The beneficial effects of the intensive breeding device for red swamp crayfish of the present invention are as follows: The intensive breeding device for red swamp crayfish of the present invention combines the habitat and mating nest structure of broodstock crayfish with the supporting broodstock rearing pond, and uses the supporting broodstock rearing management method of the present invention to achieve the separation of the living and habitat spaces of broodstock crayfish under the guidance of physical facilities. This effectively improves the space utilization rate of broodstock crayfish and larvae in the broodstock rearing pond, hatching and larval rearing pond, and greatly increases the carrying capacity and stocking density of broodstock crayfish and larvae per unit water body.

[0033] The hatching nest unit facilitates the separate rearing of individual berried crayfish. Then, based on the embryonic development stage of the fertilized eggs of the red swamp crayfish, the eggs are transferred and hatched in batches. This allows the difference in the spawning period of the berried crayfish to be controlled within one week, and fertilized eggs at the same developmental stage to be raised and hatched under the same hatching conditions. This further reduces the rearing differences between different batches of red swamp crayfish larvae and achieves the consistency of the size of the same batch of red swamp crayfish seedlings.

[0034] The beneficial effects of the intensive artificial breeding method for Procambarus clarkii of this invention are as follows: This intensive artificial breeding method for Procambarus clarkii, through supporting broodstock cultivation and management methods and two standardized screenings of broodstock at different developmental stages, achieves intensive and large-scale production of berried and juvenile Procambarus clarkii.

[0035] This method involves placing berried shrimp in independent incubation and rearing nests for centralized incubation, which greatly improves the space utilization rate of berried shrimp and hatched larvae. Compared with existing technologies, its production capacity can be increased by more than 5 times. Attached Figure Description

[0036] Figure 1 —This is a three-dimensional structural diagram of the nest structure for the habitat and mating of broodstock crayfish in an intensive breeding device for red swamp crayfish according to the present invention; Figure 2 —This is one of the schematic diagrams showing the usage status of the broodstock rearing pond in the intensive breeding device for red swamp crayfish according to the present invention; Figure 3 —This is a three-dimensional structural diagram of the nest structure for hatching and raising larvae of Procambarus clarkii in an intensive breeding device for Procambarus clarkii according to the present invention. Figure 4 —This is one of the schematic diagrams showing the usage status of the hatching and larval rearing pond in the intensive breeding device for red swamp crayfish according to the present invention; Figure 5 —This is the second schematic diagram showing the usage status of the broodstock rearing pond in the intensive breeding device for Procambarus clarkii of the present invention; Figure 6—This is the second schematic diagram showing the usage status of the hatching and larval rearing pond in the intensive breeding device for red swamp crayfish according to the present invention.

[0037] In the diagram: 1. Perching frame; 2. Partition; 3. Perching space; 4. Inner nest; 5. Perching nest; 6. Separating net; 7. Broodstock rearing pond; 8. Inlet; 9. Inlet pipe; 10. Nano-oxygenation pipe; 11. Drain; 12. Hatching nest unit; 121. Plastic netting; 122. Hatching and rearing nest; 13. Hatching and rearing frame; 131. Skeleton; 132. Crossbeam; 14. Side netting; 15. Hatching pond. Detailed Implementation

[0038] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0039] Example 1

[0040] Reference Figures 1-4 This embodiment of an intensive breeding device for Procambarus clarkii includes a nest structure for broodstock to inhabit and mate, and a nest structure for berried shrimp to hatch and raise larvae. The broodstock to inhabit and mate nest structure and the berried shrimp to hatch and raise larvae nest structure are respectively set in the broodstock breeding pond 7 and the hatching and raising pond 7. The nest structure for the parent shrimp to inhabit and mate includes a habitat frame 1 and an inner nest 4. The habitat frame 1 is a right-angled trapezoidal frame with multiple partitions 2 in the middle, dividing the habitat frame 1 into multiple habitat spaces 3 distributed in a stepped manner. The inner nest 4 is a right-angled trapezoidal body with multiple habitat cavities 5 extending through its front and back. The size of the inner nest is adapted to the habitat space 3 placed between the habitat frame 1 and the partitions 2. The hatching and larval rearing nest structure for berried shrimp includes a hatching and rearing frame 13, a hatching nest unit 12, and a side net 14. The hatching and rearing frame 13 includes a skeleton 131 and a crossbeam 132, with the crossbeam 132 overlapping the skeleton 131. The shape of the hatching nest unit 12 is adapted to the hatching and rearing frame 13, and it is assembled from plastic mesh 121, with a through hatching and rearing nest 122 in the middle. The side net 14 is placed on the front and rear sides of the hatching and rearing nest 122 to close the front and rear ends of the hatching and rearing nest 122.

[0041] The skeleton 131 is a cuboid assembled from PVC pipes.

[0042] Preferably, the upper base of the right-angled trapezoidal frame is 15-20cm long, the lower base is 35-40cm long, and the height is 25-30cm, with a distance of 5-6cm between adjacent partitions 2. The opening of the perching nest 5 is 6-8cm long and 5-6cm wide.

[0043] The incubation and cultivation frame 13 is 35-40cm long, 13-15cm wide, and 35-40cm high; the plastic mesh 121 has a mesh size of 0.1-0.2cm; and the opening of the incubation and cultivation nest 122 is 5-7cm long and 4-6cm wide.

[0044] The broodstock shrimp rearing pond 7 includes a long trough-shaped pond, an inlet and outlet drainage device, and an aeration device. Two partition nets 6 are set in the center of the long trough-shaped pond, with the sides of the partition nets 6 abutting against the long trough-shaped pond and the distance between the two partition nets 6 being 3-5cm. The inlet and outlet drainage device includes an inlet pipe 9, an inlet 8, a drainage pipe, and an outlet 11. The inlet pipe 9 is arranged along the edge of the long trough-shaped pond, and the drainage pipe is buried at the bottom of the long trough-shaped pond. The inlet 8 and the outlet 11 are connected to the inlet pipe 9 and the drainage pipe, respectively, and the inlet 8 is higher than the outlet 11. The aeration device is arranged parallel to the bottom, sides, and intervals of the partition nets 6 of the long trough-shaped pond.

[0045] An oxygenation device is installed in the broodstock rearing pond 7, which can effectively provide dissolved oxygen in the broodstock rearing pond 7, ensuring that the dissolved oxygen content in the water of the broodstock rearing pond 7 is high, meeting the growth requirements of high-density cultured Procambarus clarkii, and thus achieving the purpose of high-density intensive seedling breeding.

[0046] The oxygenation device includes at least three nano-oxygenation pipes 10.

[0047] The long, trough-shaped water tank is equipped with a shade net, which has a shading rate of 60-70%.

[0048] The hatching and larval rearing pool includes a hatching pool 15, an inlet and outlet drainage device, and an oxygenation device. The oxygenation device includes several nano-oxygenation pipes 10, which are laid parallel to each other at the bottom of the hatching pool 15.

[0049] The spacing between adjacent nano-oxygenation pipes 10 is 15~20cm.

[0050] In one exemplary embodiment, the trough-shaped pool is 80-100cm wide, 80cm high, and 3m long; the mesh size of the partition 6 is 0.8-1.5cm; the inlet 8 and outlet 11 are spaced 1-1.5m apart; and the hatching pool 15 is 100cm wide, 80cm high, and 3m long.

[0051] The high-density arrangement of nano-oxygenation pipes 10 in the hatching pond 15 significantly improved the oxygen uptake difficulty of juvenile red swamp crayfish in the hatching pond 15 and increased their survival rate.

[0052] Comparative Example 1

[0053] In a 5×4×1 meter pool, three 1×1×0.8m net cages were placed. The mesh size of the net cages was 1cm. Black plastic anti-escape film was hung around the net cages. The cages contained artificial breeding nest structures for crayfish farming in sandy soil in arid areas of Northwest China, as disclosed in CN 223437676 U. The water was filled to 40cm, and 120 parent crayfish were placed in each net cage.

[0054] Records and statistics show that one hour after introducing broodstock shrimp, the distribution rate of broodstock shrimp in the first and second layers was over 90%, while the distribution rate in the third and higher layers was less than 10%. Twenty-four hours after introduction, the distribution rate in the respiratory platform was approximately 30%, in the first and second layers it was 50%, and in the remaining space it was approximately 20%. The daily feed intake was 2% of the broodstock shrimp's body weight, and the feeding rate was below 30% three hours after feeding. Seven days after introduction, the mortality rate of the broodstock shrimp approached 50%, making it difficult to achieve high-density, continuous broodstock rearing.

[0055] Comparative Example 2

[0056] According to the current requirements for broodstock rearing in artificial breeding of crayfish fry, three cement ponds with a size of 3×4×1m are prepared as broodstock rearing ponds 7. The broodstock rearing ponds 7 are equipped with an aeration system, which includes disc-shaped nano-aeration tubes placed at the bottom of the pond. Four disc-shaped nano-aeration tubes are placed in each hatching pond 15. The water is filled to 40 cm. 360 broodstock are put into each broodstock rearing pond 7, including 240 females. The broodstock size is 18-25 grams. Several hiding places are placed in the broodstock rearing ponds 7. Feed the crayfish with 36% crude protein compound feed once a day, with the daily feeding amount accounting for 3% of the body weight of the parent crayfish. Sprinkle Bacillus and Lactic Acid Bacteria once every 5 days. Change the water once every 5 days, with the amount of water changed each time controlled at 5%. Observe and test the water quality every day and control the water quality conditions as follows: water temperature 25±1℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, and nitrite ≤0.05mg / L.

[0057] The breeding cycle was 75 days. The number of egg-bearing shrimp was counted, and a total of 331 egg-bearing shrimp were obtained, with 317 non-egg-bearing female shrimp. The first batch egg-bearing rate was 51.1%, which means that about 9 egg-bearing shrimp were produced per square meter of water surface per batch.

[0058] Comparative Example 3

[0059] Three cement ponds, each measuring 3×4×1m, were prepared as hatching ponds 15. Each hatching pond 15 was equipped with an aeration system, consisting of four disc-shaped nano-aeration tubes placed at the bottom of each pond 15. Hatching net cages, 1×1×0.8m in size and with a 2mm mesh size, were placed inside each hatching pond 15. Fifty berried shrimp were stocked in each net cage, with each cage weighing 18-25 grams. Appropriate hiding places were provided within the net cages. Hatching management and juvenile shrimp rearing management were conducted according to CN 117652440 A, "A Crayfish Breeding Device and Method." The juvenile shrimp were collected when they had completely separated from their mothers and reached a body length of 0.8-1 cm.

[0060] During the larval rearing process, due to unstable water quality and bacterial infection, the mortality rate of larvae in one of the hatching ponds 15 was high and was not included in the statistics. The remaining two hatching ponds 15, with a total of 6 hatching cages, collected approximately 44,000 juvenile shrimp, which is about 7,330 shrimp per square meter of water surface.

[0061] Example 2

[0062] A method for intensive artificial breeding of red swamp crayfish, using the above-mentioned intensive breeding device for red swamp crayfish, includes four broodstock breeding ponds 7, each 9 meters long and 80 centimeters wide.

[0063] Prepare three incubation and larval rearing pools, each 3 meters long and 1 meter wide.

[0064] The intensive artificial breeding method for Procambarus clarkii includes the following steps: S1. Preparation and pretreatment of intensive breeding equipment for red swamp crayfish; Before the broodstock shrimp are placed in the broodstock rearing pond 7, a broodstock habitat and mating nest structure is arranged in the broodstock rearing pond 7, with the back of the broodstock habitat and mating nest structure connected to the middle partition net 6 of the broodstock rearing pond 7; and a shade net (shading rate of 60-70%) is set above the broodstock rearing pond 7. One week before the collection and transfer of berried shrimp, berried shrimp hatching and larval rearing nest structures are arranged in hatching pond 15. The berried shrimp hatching and larval rearing nest structures are placed in the hatching pond 15 in parallel front to back, and the adjacent berried shrimp hatching and larval rearing nest structures are spaced 3-5cm apart. The prepared broodstock shrimp rearing pond 7 and hatching pond 15 were cleaned and disinfected. S2. Daily Management of Broodstock Shrimp Cultivation: The water level in the broodstock rearing pond 7 should be maintained at 30-40cm, and oxygenation should be increased until the water surface is slightly undulating. The same batch of broodstock shrimp whose gonads have developed to stage II (pale yellow) were placed in broodstock rearing pond 7 for gonad enhancement rearing; the size of the broodstock shrimp was 18g-25g, and their appendages were intact and undamaged; the number of broodstock shrimp was 100-150 per square meter of water surface, and the female-to-male ratio was controlled at 2-2.5:1. The broodstock of *Procambarus clarkii* were fed once daily with a formulated feed containing 36% crude protein. The daily feeding amount was 2 ± 0.5% of the broodstock's body weight, and the feeding time was from 4:00 PM to 6:00 PM. In addition to the daily feeding of the formulated feed, every 3 days, fermented feed and fresh feed were fed once each. On the 4th day, fermented feed using lactic acid bacteria as the fermentation starter was fed once, at the same amount as the formulated feed. On the 5th day, fresh feed was fed once, at 5% of the broodstock's body weight. Regularly check the gonadal development of broodstock shrimp, once every 15 days in the early stage and once every 5 days in the later stage, to observe whether the gonads have developed to stage V as a whole; when the number of broodstock shrimp in the breeding pond that have developed to stage V reaches more than 80%, the broodstock shrimp in the breeding pond are subjected to pre-spawning environmental stimulation by the dry pond transfer method. Water should be changed every five days. Aeration should be turned off ten minutes before water change. Bottom drainage should be used for water change, and each water change should replace about one-fifth of the total water volume. After water change, activated Bacillus subtilis should be sprinkled throughout the pond. During the broodstock shrimp rearing period, the water quality conditions are as follows: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, and nitrite ≤0.05mg / L.

[0065] S3. Daily management of berried shrimp hatching: Brooding shrimp were collected in batches: brooding shrimp were collected in batches according to their embryonic development stage in the parent shrimp rearing pond and transferred to the hatching pond (15) for rearing; Check the berried shrimp and embryonic development in the hatching tank every two days. If any berried shrimp die or other diseases occur, isolate and treat them in a timely manner. The water level in hatching pond 15 should be maintained at 40cm. Activated Bacillus and Oocystis should be sprayed throughout the pond. Ammonium chloride should be added at a dosage of 4 ppm, potassium dihydrogen phosphate at a dosage of 1 ppm, and molasses at a dosage of 60 ppm. Aeration should be increased until the water surface is slightly boiling. The relevant physicochemical indicators of the water should be monitored daily, and small amounts of ammonium chloride, molasses, phosphate fertilizer, and trace elements should be added as needed. The water quality conditions should be maintained as follows: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water not lower than 100mg / L.

[0066] S4. Daily Management of Larval Fry: During the later stages of hatching of the red swamp crayfish, the feeding management during the larval rearing process is as follows: when about 40% of the larvae have hatched, use a 100-mesh net bag to grind and dissolve the special compound feed for crayfish larvae (38% crude protein content) in water and then sprinkle it throughout the pond at a dosage of 2 ppm. At the same time, molasses should be added at a dosage of 2 ppm once a day. Once all larvae have emerged from the membrane and 10% have shown signs of separation, mix shrimp larvae-specific formulated feed (38% crude protein content) and yeast-based starter feed in a 3:2 ratio. Use 4 ppm of the mixed feed, grind it in a 100-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 4 ppm. Repeat this process once a day until all larvae have shown signs of separation. Then, mix shrimp larvae-specific formulated feed (38% crude protein content), black shrimp flakes, and yeast-based starter feed in a 4:3:3 ratio. Use 5 ppm of the mixed feed, grind it in an 80-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 5 ppm. Repeat this process once a day until all larvae have separated from the membrane and have grown to juvenile shrimp (0.8-1 cm in length).

[0067] During the juvenile rearing period, relevant physicochemical indicators of the water body are monitored daily. Small amounts of ammonium chloride, phosphate fertilizer, and trace elements are added as needed. Activated Bacillus and Oocystis are added regularly to ensure the following water quality conditions: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water body not lower than 100mg / L.

[0068] The disinfection process is as follows: After cleaning, add water to the broodstock breeding pond 7 and hatching pond 15 to a water level of 40cm, and sprinkle trichloroisocyanuric acid at a dosage of 0.5-1ppm (based on available chlorine). Let it stand and soak for 24 hours. After standing and soaking, turn on the aeration device until the water surface is boiling, and fully aerate for 24 hours until the residual chlorine content is lower than 0.1ppm.

[0069] In step S2, before placing the broodstock shrimp into the broodstock rearing pond 7, they are soaked in a 50 ppm chlorine dioxide solution for 5 minutes. The broodstock shrimp in this batch refer to a population of *Procambarus clarkii* raised in the same production environment from the larval rearing stage to the later growth stage, and the spawning cycle of this batch of broodstock shrimp is completed within one week. The daily feeding amount of the formulated feed for the broodstock shrimp is based on 2% of the broodstock shrimp's body weight, and is adjusted daily according to the amount of uneaten feed the following morning.

[0070] In step S2, pre-spawning environmental stimulation is performed using the dry-pond transfer method before the transfer. The specific operation of the dry-pond transfer method is as follows: first, drain the broodstock rearing pond 7; then collect and select broodstock, retaining only the females; and transfer the selected females to another broodstock rearing pond 7. Each batch of broodstock is transferred within 3-5 hours. After the dry-pond transfer, the broodstock are temporarily raised for one week according to the daily management methods for broodstock rearing.

[0071] After a week of temporary rearing, the routine collection of berried shrimp begins. In step S3 of this routine collection phase, the berried shrimp are collected and transferred in batches as follows: Berried shrimp are collected every 7 days, and the collected shrimp are transferred to the hatching pond 15 in batches according to the embryonic development stage of the fertilized eggs. The berried shrimp are then placed into hatching and rearing nests 122, with only one parent shrimp in each nest. After placement, the openings on both sides of the hatching and rearing nest 122 are sealed with side nets 14, and the hatching and larval rearing nest structure is placed into the hatching pond 15.

[0072] The collected berried shrimp were transferred to the hatching pond 15 in batches according to the embryonic development stage of the fertilized eggs. Specifically, the berried shrimp were transferred in batches according to the different stages of embryonic development of the fertilized eggs: pre-gastrulous stage, nauplius stage, and pre-embryonic stage. It is preferable to transfer them to the hatching pond 15 in 3 batches, that is, the berried shrimp whose embryonic development is in the pre-gastrulous stage, nauplius stage, and pre-embryonic stage are transferred in batches.

[0073] This invention, by transferring berried shrimp to the hatching pond 15 in batches, can control the difference in the spawning period of the produced red swamp crayfish to within one week, ensuring higher consistency in the size of the seedlings in the later batches. This is beneficial for the selection and cultivation of subsequent broodstock shrimp, thereby improving the farming capacity of red swamp crayfish.

[0074] Using the intensive artificial breeding method for *Procambarus clarkii* in this embodiment, 860 broodstock crayfish were placed in each broodstock rearing pond 7, including 520 females. Records and statistics showed that one hour after placement, approximately 70% of the broodstock inhabited the first and second layers of their mating nests (layer 3), while approximately 30% were found in the third or higher layers. Twenty-four hours after placement, the distribution rate in the third and higher layers reached approximately 50%, achieving a near-uniform distribution. The daily feed intake was 2% of the broodstock's body weight, and the feeding rate was 100% three hours after feeding. Seven days after placement, the mortality rate of the broodstock was less than 5%. The subsequent experimental period was 73-75 days. The number of broodstock shrimp carrying eggs was counted. A total of 1,329 broodstock shrimp were obtained from the four breeding ponds, and 543 non-broodstock female shrimp were obtained. The first batch egg-carrying rate was 71%, and about 46 broodstock shrimp were produced per square meter of water surface per batch. Compared with the breeding method in control example 2, which did not use the breeding nest structure of broodstock shrimp habitat and mating, the production capacity of the intensive artificial breeding method of red swamp crayfish in this example was increased by more than 5 times.

[0075] Each hatching and larval rearing pond contained 1125 berried crayfish. The larvae were collected once they had completely separated from their mothers and reached a length of 0.8-1 cm. Records and sampling showed a total of approximately 525,000 larvae collected, yielding approximately 58,000 larvae per square meter of water surface. Compared to the control example 3, which did not utilize berried crayfish hatching and larval rearing nest structures, the intensive artificial breeding method for *Procambarus clarkii* in this example yielded 7.91 times the larval production of the conventional method.

[0076] This invention discloses an intensive breeding device for *Procambarus clarkii*. The specific dimensions of the broodstock rearing pond 7, the hatching and larval rearing pond, and the dimensions of the broodstock habitat and mating nest structures, as well as the hatching and larval rearing nest structures, can be adjusted according to actual conditions and are not limited to the specific dimensions shown in embodiments 1 and 2 above. If the broodstock rearing pond 7 and the hatching pond 15 are relatively long (e.g., 12 meters long), multiple sets of broodstock habitat and mating nest structures, and hatching and larval rearing nest structures can be used in combination. See details... Figure 5 and Figure 6 The diagram illustrates a common arrangement structure. Similarly, when the width and length of the broodstock rearing pond 7 and the hatching pond 15 are both large, the broodstock habitat and mating nest structures, as well as the berried shrimp hatching and larval rearing nest structures, can be arranged in an array within the broodstock rearing pond 7 and the hatching pond 15. At the same time, the aeration devices are added in an array to facilitate feeding and broodstock transfer. The changes in the above technical features can be understood and implemented by those skilled in the art through textual description, and therefore no further drawings are provided.

[0077] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

Claims

1. An intensive artificial breeding method for Procambarus clarkii, characterized in that, An intensive breeding device for red swamp crayfish was used. The intensive breeding device for the red swamp crayfish includes a nest structure for broodstock to inhabit and mate, and a nest structure for berried crayfish to hatch and raise larvae. The nest structure for broodstock to inhabit and mate, and the nest structure for berried crayfish to hatch and raise larvae are respectively set in the broodstock breeding pond (7) and the hatching and raising pond. The structure of the parent shrimp's habitat and mating nest includes a habitat frame (1) and an inner nest (4). The habitat frame (1) is a right-angled trapezoidal frame with multiple partitions (2) in the middle, dividing the habitat frame (1) into multiple habitat spaces (3) distributed in a stepped manner. The inner nest (4) is a right-angled trapezoidal body with multiple habitat nests (5) that run through its front and back. The size of the inner nest is adapted to the habitat space (3) placed between the habitat frame (1) and the partitions (2). The hatching and larval rearing nest structure for berried shrimp includes a hatching and rearing frame (13), a hatching nest unit (12), and a side net (14). The hatching and rearing frame (13) includes a skeleton (131) and a crossbeam (132), with the crossbeam (132) overlapping the skeleton (131). The shape of the hatching nest unit (12) is adapted to the hatching and rearing frame (13), and it is assembled from plastic mesh (121), with a through hatching and rearing nest (122) in the middle. The side net (14) is placed on the front and rear sides of the hatching and rearing nest (122) to close the front and rear ends of the hatching and rearing nest (122). The intensive artificial breeding method for the red swamp crayfish includes the following steps: S1. Preparation and pretreatment of intensive breeding equipment for Procambarus clarkii: Before the parent shrimp are placed in the parent shrimp rearing pond (7), the parent shrimp habitat and mating nest structure is arranged in the parent shrimp rearing pond (7). One week before the collection and transfer of berried shrimp, the berried shrimp hatching and larval rearing nest structure is arranged in the hatching pond (15). The prepared broodstock breeding pond (7) and hatching pond (15) were cleaned and disinfected. S2. Daily Management of Broodstock Shrimp Cultivation: The water level in the broodstock rearing pond (7) should be controlled at 30-40cm, and oxygenation should be increased until the water surface is rippled. The same batch of broodstock shrimp that have developed to stage II gonads are placed in the broodstock breeding pond (7) for gonad enhancement breeding; the size of the broodstock shrimp is 18g-25g; the number of broodstock shrimp is 100-150 per square meter of water surface, and the ratio of female to male is controlled at 2-2.5:1; Regularly check the gonadal development of broodstock shrimp, once every 15 days in the early stage and once every 5 days in the later stage, to observe whether the gonads have developed to stage V as a whole; when the number of broodstock shrimp in the breeding pond that have developed to stage V reaches more than 80%, the broodstock shrimp in the breeding pond are subjected to pre-spawning environmental stimulation by the dry pond transfer method. Feed the broodstock of *Procambarus clarkii* once daily with a formulated feed, at a rate of 2 ± 0.5% of the broodstock's body weight, between 4:00 PM and 6:00 PM. After three days of feeding with the formulated feed, on the fourth day, feed the broodstock with fermented feed containing lactic acid bacteria, at the same rate as the formulated feed. On the fifth day, feed the broodstock with fresh feed once daily, at a rate of 5% of their body weight. Water should be changed every five days. Aeration should be turned off ten minutes before water change. Bottom drainage should be used for water change, and each water change should replace one-fifth of the total water volume. After water change, activated Bacillus subtilis should be sprinkled throughout the pond. During the broodstock shrimp rearing period, the water quality conditions are as follows: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, and nitrite ≤0.05mg / L. S3. Daily management of berried shrimp hatching: Based on the embryonic development stage of the broodstock shrimp in the breeding pond, the broodstock shrimp were collected in batches and transferred to the hatching pond (15) for further cultivation; Check the berried shrimp and embryonic development in the hatching tank every two days. If any berried shrimp die or other diseases occur, isolate and treat them in a timely manner. The water level in the hatching pond (15) should be controlled at 40cm. Activated Bacillus and Oocystis should be sprinkled throughout the pond. Add ammonium chloride at a dosage of 4ppm, potassium dihydrogen phosphate at a dosage of 1ppm, and molasses at a dosage of 60ppm. Aerate the water until it is slightly boiling. Monitor the relevant physicochemical indicators of the water body daily, and add a small amount of ammonium chloride, molasses, phosphate fertilizer and trace elements as needed. Ensure that the water quality conditions are: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water body not lower than 100mg / L. S4. Daily Management of Larval Fry: During the later stages of hatching of the red swamp crayfish, the feeding management during the larval rearing process is as follows: when 40% of the larvae have hatched, use a 100-mesh net bag to grind the special compound feed for crayfish larvae and dissolve it in water, then sprinkle it throughout the pond at a dosage of 2 ppm. At the same time, add molasses at a dosage of 2 ppm once a day. After all the larvae have emerged from the membrane and 10% of them show signs of separation, mix shrimp larvae-specific formulated feed and yeast-based starter feed in a 3:2 ratio. Use 4 ppm of the mixed feed, grind it in a 100-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 4 ppm. Repeat this process once a day. After all the larvae show signs of separation, mix shrimp larvae-specific formulated feed, black shrimp flakes, and yeast-based starter feed in a 4:3:3 ratio. Use 5 ppm of the mixed feed, grind it in an 80-mesh mesh bag, dissolve it in water, and then sprinkle it throughout the pond. At the same time, add molasses at a dosage of 5 ppm. Repeat this process once a day until all the larvae have separated from the membrane and have grown into juvenile shrimp with a body length of 0.8-1 cm. During the juvenile rearing period, relevant physicochemical indicators of the water body are monitored daily. Small amounts of ammonium chloride, phosphate fertilizer, and trace elements are added as needed. Activated Bacillus and Oocystis are added regularly to ensure the following water quality conditions: water temperature 22-28℃, dissolved oxygen ≥5mg / L, pH value 7.5-8.5, ammonia nitrogen ≤0.2mg / L, nitrite ≤0.05mg / L, and total alkalinity of the water body not lower than 100mg / L.

2. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 1, characterized in that, The skeleton (131) is a cuboid assembled from PVC pipes.

3. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 1 or 2, characterized in that, The broodstock breeding pond (7) includes a long trough-shaped pool, an inlet and outlet device, and an aeration device. Two partition nets (6) are set in the center of the long trough-shaped pool. The sides of the partition nets (6) are in contact with the long trough-shaped pool, and the distance between the two partition nets (6) is 3-5cm. The inlet and outlet device includes an inlet pipe (9), an inlet (8), a drain pipe, and a drain outlet (11). The inlet pipe (9) is arranged on the edge of the long trough-shaped pool, and the drain pipe is buried at the bottom of the long trough-shaped pool. The inlet (8) and the drain outlet (11) are connected to the inlet pipe (9) and the drain pipe, respectively, and the inlet (8) is higher than the drain outlet (11). The aeration device is arranged in parallel at the bottom, sides, and intervals of the partition nets (6) of the long trough-shaped pool.

4. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 3, characterized in that, The oxygenation device includes at least three nano-oxygenation pipes (10).

5. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 3, characterized in that, The long, trough-shaped water tank is equipped with a shade net, which has a shading rate of 60-70%.

6. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 1 or 2, characterized in that, The incubation and larval rearing pool includes an incubation pool (15), an inlet and outlet device, and an oxygenation device. The inlet and outlet device includes an inlet pipe (9), an inlet (8), an outlet pipe, and an outlet (11). The inlet pipe (9) is arranged along the edge of the incubation pool (15), and the outlet pipe is buried at the bottom of the incubation pool (15). The inlet (8) and outlet (11) are connected to the inlet pipe (9) and the outlet pipe (11), respectively, and the inlet (8) is higher than the outlet (11). The oxygenation device includes several nano-oxygenation pipes (10), which are laid parallel to each other at the bottom of the incubation pool (15).

7. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 6, characterized in that, The spacing between adjacent nano-oxygenation pipes (10) is 15~20cm.

8. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 1, characterized in that, In step S1, the disinfection operation is as follows: after cleaning, add water to the broodstock breeding pond (7) and hatching pond (15) to a water level of 40cm, and sprinkle trichloroisocyanuric acid at a dosage of 0.5-1ppm. Let it stand and soak for 24 hours. After standing and soaking, turn on the aeration device until the water surface is boiling. Aerate fully for 24 hours until the residual chlorine content is less than 0.1ppm. In step S2, before the broodstock is placed in the broodstock breeding pond (7), it is soaked in a 50ppm chlorine dioxide solution for 5 minutes; the same batch of broodstock refers to the population of red swamp crayfish that has been cultivated in the same production environment from the larval stage, the growth stage to the later stage of growth, and the spawning cycle of the corresponding batch of broodstock is completed within one week. The specific operation of the dry pond transfer method is as follows: first drain the broodstock breeding pond (7), then collect and select broodstock, keep only the female shrimp, and transfer the selected female shrimp to another broodstock breeding pond (7). Each batch of broodstock is controlled to be completed within 3-5 hours in the entire transfer process; after the dry pond transfer, the broodstock are temporarily raised for one week according to the daily management method of broodstock breeding. In step S3, the operation of collecting and transferring berried shrimp in batches is as follows: berried shrimp are collected every 7 days, and the collected berried shrimp are transferred to the hatching pond (15) in batches according to the embryonic development stage of the fertilized eggs; the berried shrimp are placed in the hatching and rearing nests (122) respectively, and only one parent shrimp is placed in each hatching and rearing nest (122). After the berried shrimp are placed, the openings on both sides of the hatching and rearing nest (122) are sealed with side nets (14), and the berried shrimp hatching and larval rearing nest structure is placed in the hatching pond (15).

9. The intensive artificial breeding method for *Procambarus clarkii* as described in claim 8, characterized in that, The collected berried shrimp were transferred to the hatching pond (15) in batches according to the embryonic development stage of the fertilized eggs. Specifically, the berried shrimp were transferred in three batches according to the three different stages of embryonic development of the fertilized eggs: pre-gastrul stage, nauplius stage, and pre-embryonic stage.

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