Regulator for promoting blooming of bougainvillea speetabilis or inhibiting bud shedding

Abstract

The invention provides a regulator for promoting bougainvillea speetabilis to bloom or inhibiting buds from falling off, and relates to the technical field of plant regulators. The regulator provided by the invention is selected from one or more of 0.1 to 0.3 mg / L of fluid boron, 1 to 3 mg / L of KH2PO4, 5 to 25 mg / L of NAA and 50 mg / L of sugar alcohol calcium. According to the invention, fluid boron, KH2PO4, NAA and sugar alcohol calcium are selected and compounded, and under natural production and cultivation conditions and dark conditions of simulated storage and transportation, comprehensive and efficient regulation and control schemes for regulating and controlling the period of the bougainvillea speetabilis and inhibiting shedding are respectively screened out; the regulator is applied through exogenous spraying, flowering of the bougainvillea speetabilis can be well promoted or bud falling is inhibited, the ornamental value and storage and transportation resistance of the bougainvillea speetabilis are improved, and a basis is provided for production, cultivation, management and transportation in practical application of the bougainvillea speetabilis.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant regulators, in particular to a regulator for promoting the flowering of Bougainvillea or inhibiting the shedding of flower buds. BACKGROUND

[0002] Bougainvillea spectabilis Willd. Bougainvillea spectabilis Willd. Also known as leaf flower, nine heavy cloth, and treasure cloth flower, the evergreen vine-shaped ornamental shrub is widely planted in tropical and subtropical regions due to its long flowering period, rich flower colors, abundant varieties, and strong adaptability. It is also widely planted in southern China and has high ornamental value. However, in daily production cultivation and application, some Bougainvillea varieties have bright flower bud colors but short flowering periods, inconsistent flowering times, and other factors that do not achieve good ornamental effects. In low light and rainy environments, the flowers often fall, greatly affecting the ornamental and commercial value of Bougainvillea. In addition, during transportation, weak light and bumpy carriage environments can also cause Bougainvillea to shed flower buds, making it less resistant to storage and transportation, which is not conducive to long-distance transportation and has a significant impact on the commercial value and economic value of Bougainvillea, which is an important factor restricting the development of the Bougainvillea industry.

[0003] Currently, the main methods for regulating the flowering period of Bougainvillea are physical control methods such as water control and pruning, and there is little research on the application of plant growth regulators and nutrient element regulation. Therefore, to address the inconsistent flowering periods, flower bud shedding, and poor storage and transportation of Bougainvillea, a plant growth regulator that can promote flowering or inhibit flower bud shedding is developed to comprehensively and efficiently regulate the flowering period and inhibit shedding. This method can solve the problem of strong ornamental but short flowering period and poor transportation resistance of Bougainvillea to some extent, providing a theoretical basis for the production and cultivation of Bougainvillea, improving its application rate in parks, streets, and festival activities, and broadening the market application prospects of Bougainvillea.

[0004] The prior art CN117136953A discloses a bougainvillea leaf anti-shedding agent and a preparation method and application thereof, an efficacy solution is obtained by compounding calcium sugar alcohol and alpha-naphthalene acetic acid, and then a surfactant Tween is added in the efficacy solution to obtain the anti-shedding agent, which can effectively reduce the shedding rate of bougainvillea leaves during transportation and after transportation, but the shedding rate of bougainvillea leaves under natural conditions is not studied, and the influence on bougainvillea flowering is not studied, the function is single, only the flower leaves can be prevented from falling off, and the bougainvillea may face the fundamental problems of less flowering and irregular flowering period, and the application is limited. The prior art CN116711731A discloses a regulator for prolonging the flowering period of bougainvillea and a use method thereof, the regulator comprises 8% p-chlorophenoxyacetic acid, 20% naphthalene acetic acid, 1% 6-benzylaminopurine and 98% potassium dihydrogen phosphate, the use method can effectively prolong the flowering period of bougainvillea, can improve the growth trend and storage resistance of bougainvillea even in a sealed environment, and reduces the problems of flower and leaf abscission of bougainvillea. However, the regulator is complex in compounding, it is difficult to accurately control, unpredictable physiological stress may be generated on different varieties and different growth states of bougainvillea, the risk of drug damage is increased, the cost is high, and the process requirement is also high.

[0005] Therefore, it is of practical significance to provide a plant growth regulator capable of promoting flowering or inhibiting bud abscission, which is low in cost and can comprehensively and efficiently regulate the flowering period and inhibit shedding of bougainvillea. SUMMARY

[0006] Therefore, the present application provides a regulator for promoting flowering or inhibiting bud abscission of bougainvillea.

[0007] The technical scheme of the present application is as follows: The regulator for promoting flowering or inhibiting bud abscission of bougainvillea is selected from one or more of 0.1-0.3 mg / L fluid boron, 1-3 mg / L KH2PO4, 5-25 mg / L NAA and 50 mg / L calcium sugar alcohol.

[0008] Further, the regulator promotes flowering or inhibits bud abscission of bougainvillea under natural conditions or simulated storage and transportation conditions.

[0009] Further, the natural conditions are that the temperature is 22-26 DEG C, the sunshine duration is 10-12 h, and the relative humidity is 74%-82%.

[0010] Further, the simulated storage and transportation conditions are that after the plant to be sprayed is dried, the plant is moved into a dark room, the temperature is kept at 24-26 DEG C, the relative humidity is kept at 72%-80%, the transportation is simulated for 2-4 days, after the dark treatment is completed, the plant is placed under natural conditions, and the sunshine duration is 10-12 h.

[0011] Further, the regulator for promoting the blooming of the bougainvillea under natural conditions is composed of 0.2 mg / L fluid boron, 25 mg / L NAA and 50 mg / L calcium sugar alcohol.

[0012] Further, the regulator for inhibiting the bud drop under natural conditions is 25 mg / L NAA and 50 mg / L calcium sugar alcohol.

[0013] Further, the regulator for promoting the blooming of the bougainvillea under simulated storage and transportation conditions is 2 mg / L KH2PO4.

[0014] Further, the regulator for inhibiting the bud drop under simulated storage and transportation conditions is 0.3 mg / L fluid boron.

[0015] Further, the bougainvillea is sprayed with the regulator until water drops fall on the leaves and the buds.

[0016] Further, the regulator is mixed with 0.1% Tween 20 before spraying.

[0017] Compared with the prior art, the present application has the following beneficial effects: 1. The present application selects and compounding fluid boron, KH2PO4, NAA and calcium sugar alcohol, and screens out comprehensive and efficient regulation schemes for the flowering and bud drop inhibition of the bougainvillea under natural production and cultivation conditions and simulated storage and transportation dark conditions. The regulator applied by external spraying can promote the blooming or inhibit the bud drop of the bougainvillea, improve the ornamental and storage and transportation properties of the bougainvillea, and provide a basis for the production, cultivation management and transportation of the bougainvillea in practical application.

[0018] 2. According to the verification of the examples, the regulator composed of 0.2 mg / L fluid boron, 25 mg / L NAA and 50 mg / L calcium sugar alcohol has the best effect on promoting the blooming of the bougainvillea under natural conditions; the regulator composed of 25 mg / L NAA and 50 mg / L calcium sugar alcohol has the most significant effect on inhibiting the bud drop at each stage of the flowering period. Under simulated storage and transportation conditions, the single application of 2 mg / L KH2PO4 has the best effect on promoting the blooming of the bougainvillea; the single application of 0.3 mg / L fluid boron has the most significant effect on inhibiting the bud drop at each stage of the flowering period. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The figure is for the classification of the bracts of the bougainvillea 'Miss Manila'.

[0020] Figure 2 The figure is for the influence of examples 1-3 on the blooming rate of the bougainvillea under natural conditions, wherein a is the blooming rate of the buds after 3 days of treatment, and b is the blooming rate of the buds after 6 days of treatment.

[0021] Figure 3 Figure showing the effect of Examples 4-6 on the flowering rate of Bougainvillea under natural conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0022] Figure 4 Figure showing the effect of Examples 7-9 on the flowering rate of Bougainvillea under natural conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0023] Figure 5 Figure showing the effect of different plant growth regulators on the flowering rate of Bougainvillea under natural conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0024] Figure 6 Figure showing the effect of Examples 1-3 on the flower bud drop rate of Bougainvillea under natural conditions, where a is the A class flower bud drop rate, b is the B class flower bud drop rate, c is the C class flower bud drop rate and d is the D class flower bud drop rate.

[0025] Figure 7 Figure showing the effect of Examples 1-3 on the plant drop of Bougainvillea under natural conditions.

[0026] Figure 8 Figure showing the effect of Examples 4-6 on the flower bud drop rate of Bougainvillea under natural conditions, where a is the A class flower bud drop rate, b is the B class flower bud drop rate, c is the C class flower bud drop rate and d is the D class flower bud drop rate.

[0027] Figure 9 Figure showing the effect of Examples 4-6 on the plant drop of Bougainvillea under natural conditions.

[0028] Figure 10 Figure showing the effect of Examples 7-9 on the flower bud drop rate of Bougainvillea under natural conditions, where a is the A class flower bud drop rate, b is the B class flower bud drop rate, c is the C class flower bud drop rate and d is the D class flower bud drop rate.

[0029] Figure 11 Figure showing the effect of Examples 7-9 on the plant drop of Bougainvillea under natural conditions.

[0030] Figure 12 Figure showing the effect of different plant growth regulators on the flower bud drop rate of Bougainvillea under natural conditions.

[0031] Figure 13 Figure showing the effect of Examples 10-12 on the flowering rate of Bougainvillea under simulated storage and transport conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0032] Figure 14Figure showing the effect of Examples 13-15 on the flowering rate of Bougainvillea under simulated storage and transport conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0033] Figure 15 Figure showing the effect of Examples 16-18 on the flowering rate of Bougainvillea under simulated storage and transport conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0034] Figure 16 Figure showing the effect of Examples 19-21 on the flowering rate of Bougainvillea under simulated storage and transport conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0035] Figure 17 Figure showing the effect of different plant growth regulators on the flowering rate of Bougainvillea under simulated storage and transport conditions, where a is the flower bud flowering rate at 3 days of treatment and b is the flower bud flowering rate at 6 days of treatment.

[0036] Figure 18 Figure showing the effect of Examples 10-12 on the rate of shedding of flower buds of different levels of Bougainvillea under simulated storage and transport conditions, where a is the rate of shedding of flower buds of level A, b is the rate of shedding of flower buds of level B, c is the rate of shedding of flower buds of level C and d is the rate of shedding of flower buds of level D.

[0037] Figure 19 Figure showing the effect of Examples 10-12 on the shedding of plants of Bougainvillea under simulated storage and transport conditions.

[0038] Figure 20 Figure showing the effect of Examples 13-15 on the rate of shedding of flower buds of different levels of Bougainvillea under simulated storage and transport conditions, where a is the rate of shedding of flower buds of level A, b is the rate of shedding of flower buds of level B, c is the rate of shedding of flower buds of level C and d is the rate of shedding of flower buds of level D.

[0039] Figure 21 Figure showing the effect of Examples 13-15 on the shedding of plants of Bougainvillea under simulated storage and transport conditions.

[0040] Figure 22 Figure showing the effect of Examples 16-18 on the rate of shedding of flower buds of different levels of Bougainvillea under simulated storage and transport conditions, where a is the rate of shedding of flower buds of level A, b is the rate of shedding of flower buds of level B, c is the rate of shedding of flower buds of level C and d is the rate of shedding of flower buds of level D.

[0041] Figure 23 Figure showing the effect of Examples 16-18 on the shedding of plants of Bougainvillea under simulated storage and transport conditions.

[0042] Figure 24The graph simulates the effect of Examples 19-21 on the shedding rate of each level of flower bud of Bougainvillea under storage and transportation conditions, wherein a is the shedding rate of A level flower bud, b is the shedding rate of B level flower bud, c is the shedding rate of C level flower bud, and d is the shedding rate of D level flower bud.

[0043] Figure 25 The graph simulates the effect of Examples 19-21 on the shedding of Bougainvillea plants under storage and transportation conditions.

[0044] Figure 26 The graph simulates the effect of different plant growth regulators on the shedding rate of each level of flower bud of Bougainvillea under storage and transportation conditions. DETAILED DESCRIPTION

[0045] In order to better understand the technical content of the present application, the following specific examples are provided to further illustrate the present application.

[0046] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.

[0047] The materials, reagents, etc. used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0048] The reagent names and manufacturers of the present application are as follows:

[0049] Examples 1-9

[0050] The preparation method of the above-mentioned regulators is as follows: Prepare 1 mg / mL NAA stock solution: weigh 0.1 g NAA and dissolve in 100 ml distilled water, store at 4°C; prepare 1 mg / mL calcium glycerol stock solution: weigh 0.1 g calcium glycerol and dissolve in 100 ml distilled water, store at 4°C; Prepare 1 mg / mL KH2PO4 stock solution: weigh 0.1 g KH2PO4 and dissolve in 100 ml distilled water, store at 4°C; Prepare 1 mg / mL fluid boron stock solution: weigh 0.1 g fluid boron and dissolve in 100 ml distilled water, store at 4°C; Prepare different concentrations of the above-mentioned regulators by using the above-mentioned stock solutions; The regulator 5 mg / L NAA + 50 mg / L calcium glycerol of Example 1 refers to 1 L of distilled water containing 5 mg of NAA and 50 mg of calcium glycerol.

[0051] The regulator 15 mg / L NAA + 50 mg / L calcium glycerol of Example 2 refers to 1 L of distilled water containing 15 mg of NAA and 50 mg of calcium glycerol.

[0052] Example 3 regulator 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0053] Example 4 regulator 0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 0.1 mg of fluid boron, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0054] Example 5 regulator 0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 0.2 mg of fluid boron, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0055] Example 6 regulator 0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 0.3 mg of fluid boron, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0056] Example 7 regulator 1 mg / L KH2PO4+ 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 1 mg of KH2PO4, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0057] Example 8 regulator 2 mg / L KH2PO4+ 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 2 mg of KH2PO4, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0058] Example 9 regulator 3 mg / L KH2PO4+ 25 mg / L NAA + 50 mg / L sugar alcohol calcium refers to 1 L of distilled water containing 3 mg of KH2PO4, 25 mg of NAA and 50 mg of sugar alcohol calcium.

[0059] Test Example 1 - under natural conditions 1. Plant material Test material: Bougainvillea glabra cv. 'Miss Manila', also called 'Shuihong' and 'Tong'anhong'.

[0060] Test material selection: Bougainvillea glabra 'Miss Manila' with basically consistent initial flowering period, healthy growth, no diseases and insect pests, similar growth conditions such as height and crown width, average plant height 90.5 cm, crown width 58.0 cm, plastic flowerpot size 30 cm (diameter) x 18 cm (bottom diameter) x 21 cm (height), and plant spacing 90 cm x 90 cm when treated, and the substrate is local garden soil: nutrient soil: coconut husk = 2:2:1.

[0061] Test site: Located in the Hongqi base of Sanya Research Institute of China Agricultural University in Sanya City, Hainan Province, natural treatment was completed in the greenhouse of Hongqi base, and dark treatment was completed in the darkroom of the base.

[0062] 2. Test method 2.1 Test treatment under natural conditions A randomized block design was used to compare the effects of Examples 1-9 on the flowering and bud drop of potted Bougainvillea spectabilis ‘Miss Manila’ under natural conditions. Spraying with water (CK) was used as a control. Tween 20 was added as a surfactant during treatment, and the addition amount of surfactant was 0.1% of the spraying regulator solution. Each group of treatment had five pots, and was repeated three times to ensure the accuracy of the experimental results and reduce experimental errors. The method of exogenous spraying with a 500 mL spray bottle was used, and five pots of plants were sprayed with 500 mL of solution each time until water droplets fell on the leaves and buds of the plants. Spraying was performed at the initial flowering stage of the plants, and was performed at about 16:00 in the afternoon on sunny days to avoid the influence of rainy weather and high temperature on the test results.

[0063] The average temperature during the natural condition treatment was 22-26°C, the average sunshine duration was 11h, and the average relative humidity was 74-82%. During the treatment period, routine maintenance and management were used, and watering was performed when the surface of the pot soil was dry, and the water was poured thoroughly, and the water flowing out of the pot bottom was appropriate.

[0064] 2.2 Determination of physiological indicators According to different stages of the flowering period of Bougainvillea spectabilis, the flower buds of Bougainvillea spectabilis were divided into four stages, A, B, C, and D, as shown in Figure 1 : A grade of flower bud is at the initial flowering stage, the bract is about 1 cm wide and about 2 cm long, and the true flower is not opened; B grade of flower bud is at the initial flowering stage, the bract is about 2 cm wide and about 2.8 cm long, and the true flower is about to open; C grade of flower bud is at the full flowering stage, the bract is about 3.1 cm wide and about 4 cm long, and the true flower is opened 1-3 flowers; D grade of flower bud is at the flower falling stage, the bract is about 3 cm wide and about 3.5 cm long, and the true flower is opened and the flower tube is curled. Before spraying treatment, the test materials were labeled according to the four stages of the bract, which were used for the determination and analysis of the opening of the small flowers and the drop of the flower buds.

[0065] After exogenous spraying treatment, every 0, 3, 6, 10d at 16:00, observation and photographing were recorded, and the number of true flower opening and flower bud drop of each stage labeled before treatment was counted, and the flowering rate and flower bud drop rate were calculated.

[0066] Flowering rate (%) = new opening number / unopened number before treatment x 100%; Flower bud drop rate (%) = flower bud drop number / total number of flower buds before treatment x 100%.

[0067] 2.3 Data processing Data processing and plotting were performed using Microsoft Excel 2019 and GraphPad Prism 9.5. Significant analysis of data was performed using Duncan method of SPSS 26.0 software (p<0.05), and significant difference was marked by letter method.

[0068] 3. Data results 3.1 Effects of different treatments on the flowering of Bougainvillea spectabilis under natural conditions 3.1.1 Effects of N1-N3 (Example 1-3) on the flowering of Bougainvillea spectabilis N1 (5 mg / L NAA + 50 mg / L sugar alcohol calcium), N2 (15 mg / L NAA + 50 mg / L sugar alcohol calcium), N3 (25 mg / L NAA + 50 mg / L sugar alcohol calcium), as shown in a of Figure 2 , when treated for 3d, the flowering rate of Bougainvillea spectabilis first decreased and then increased with the increase of NAA concentration. The flowering rate after N1 and N3 treatment was significantly higher than that of the control CK group, and the average flowering rate of N3 was 36.87%, which was significantly higher than that of the control CK group 23.90% and N 18.27%, as shown in b of Figure 2 , when treated for 6d, the flowering rate of N1, N2 and N3 was significantly higher than that of the control CK group, and the flowering rate of N3 group was 36.4% higher than that of the control CK group.

[0069] 3.1.2 Effects of E1-E3 (Example 4-6) on the flowering of Bougainvillea spectabilis E1 (0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), E3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), as shown in a of Figure 3 , when treated for 3d, the flowering rate of Bougainvillea spectabilis first increased and then decreased with the increase of fluid boron concentration. The flowering rate after E2 treatment was significantly higher than that of the control CK group 25.1%, and the flowering rate of E1 and E3 had no significant difference with the control CK group, and had no obvious promoting effect on the flowering rate of 3d. As shown in b of Figure 3 , when treated for 6d, the flowering rate of E2 and E3 was significantly higher than that of the control CK group, and the promoting effect of E2 on flowering was the most obvious, and the flowering rate of E1 had no significant difference with the control CK group.

[0070] 3.1.3 Effects of K1-K3 (Example 7-9) on the flowering of Bougainvillea spectabilis K1 (1 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), K2 (2 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), and K3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 4 As shown in Figure a, after 3 days of treatment, the flowering rate of bougainvillea increased with increasing KH₂PO₄ concentration. The flowering rate after K₃ treatment was significantly higher than that of the control group (CK), while K₂ showed no significant difference compared to the control group (CK). The flowering rate of K₁ was significantly lower than other treatment groups, with a flowering rate of only 19.4%, significantly inhibiting true flower opening. Figure 4 As shown in b, after 6 days of treatment, the flowering rates of K1, K2 and K3 treatments were significantly higher than those of the control group CK, which can promote the early flowering of bougainvillea.

[0071] In summary, the optimal concentrations that significantly promoted bougainvillea flowering in each treatment group were combined as follows: N3 (25 mg / L NAA + 50 mg / L calcium oxytocin), K3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L calcium oxytocin), and E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L calcium oxytocin). Figure 5 As shown in Figure a, after 3 days of treatment, the flowering rates of the E2, N3, and K3 treatments were all higher than the control group (CK). Among them, the E2 treatment was significantly higher than the other two treatments, showing the most significant effect in promoting flowering. However, the flowering rate of the K3 treatment was not significantly different from the control group (CK), indicating no significant effect in promoting flowering. Figure 5 As shown in Figure b, after 6 days of treatment, the flowering rate of all three treatments was significantly higher than that of the CK group, with the E2 treatment showing the most significant effect in promoting flowering.

[0072] Therefore, the E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium) treatment of the present invention showed the best flowering promotion effect at 3 days and 6 days.

[0073] 3.2 Effects of different treatments under natural conditions on inhibiting bougainvillea flower bud drop 3.2.1 Effects of N1-N3 (Examples 1-3) on inhibiting bougainvillea flower bud drop N1 (5 mg / L NAA + 50 mg / L sugar alcohol calcium), N2 (15 mg / L NAA + 50 mg / L sugar alcohol calcium), N3 (25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 6As shown in Figure a, among the grade A flower buds after each treatment, the N3 treatment had the lowest flower bud drop rate at 3.4%, which was 4.5% lower than the CK group. The drop rate was significantly lower than the CK group, while the N1 treatment had a similar flower bud drop rate to the CK group, indicating no significant effect in inhibiting flower bud drop. Figure 6 As shown in b, among the B-grade flower buds after each treatment, the N1, N2, and N3 treatments, with different concentrations of NAA, significantly inhibited the abscission rate of B-grade flower buds compared to the CK group. The abscission rate decreased with increasing NAA concentration. Among them, the N3 treatment resulted in the lowest abscission rate of 17.2%, which was 17.8% lower than the abscission rate of the CK group. Figure 6 As shown in c, among the C-grade flower buds after each treatment, the abscission rate of C-grade flower buds decreased with increasing NAA concentration. Specifically, the abscission rate of flower buds treated with N3 was significantly lower than that treated with the CK group, with the abscission rate of C-grade flower buds being 29% lower than that of the CK group, significantly inhibiting the abscission of C-grade flower buds. Figure 6 As shown in d, among the D-grade flower buds after each treatment, the abscission rate of D-grade flower buds in the three combinations of different NAA concentrations was significantly lower than that in the CK group. Among them, the N3 group had the lowest D-grade flower bud abscission rate, only 35.5%, which was 58.2% lower than that in the CK group, demonstrating a significant inhibitory effect on D-grade flower bud abscission. There was no significant difference in the flower bud abscission rate between the N2 and N3 treatments, but both significantly inhibited D-grade flower bud abscission compared to the CK group.

[0074] See Figure 7 All three treatments with different concentrations of NAA inhibited bougainvillea bud drop compared to the control group. At 3 days, the inhibitory effect among treatments was not significant. At 6 days, the combination of NAA and calcium sugar alcohol resulted in less bud drop, as shown in the figure. At 10 days, treatments N2 and N3 showed significant inhibitory effects on bud drop, significantly suppressing bud drop at stages A, B, C, and D, and prolonging the flowering period compared to the control group.

[0075] 3.2.2 Effects of E1-E3 (Examples 4-6) on inhibiting bougainvillea flower bud drop E1 (0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), E3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 8As shown in Figure a, in the A-grade flower buds treated with different concentrations of fluid boron, the flower bud abscission rate first decreased and then increased with increasing fluid boron concentration. Treatment E2 significantly inhibited flower bud abscission, with an abscission rate 4.4% lower than the CK group. Treatments E1 and E3 showed no significant inhibitory effect on flower bud abscission compared to the CK group, but E3 accelerated flower bud abscission. Figure 8 As shown in b, with the increase of fluid boron concentration, the abscission rate of grade B flower buds showed a change of first decreasing and then increasing. Among them, the abscission rates of treatments E1 and E3 were higher than those of the CK group, showing no inhibitory effect and accelerating flower bud abscission. The abscission rate of treatment E2 was 14.7% lower than that of the CK group, significantly inhibiting the abscission of grade B flower buds. Figure 8 As shown in Figure c, among the C-grade flower buds treated with different concentrations of fluid boron, treatment E2 significantly inhibited bud abscission, with an abscission rate 28.7% lower than that of the CK group. Treatments E1 and E3 showed no significant difference in abscission rates compared to the CK group, indicating no significant inhibitory effect on bud abscission, but neither did they accelerate it. Figure 8 As shown in d, all three groups of different concentrations of fluid boron treatments inhibited the abscission of D-grade flower buds. Among them, the E2 treatment had the most significant inhibitory effect, with an abscission rate of 47.7%, which was 41.9% lower than that of the CK group.

[0076] Therefore, the E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium) treatment was most effective in inhibiting the abscission of flower buds at all levels.

[0077] See Figure 9 All three groups of different concentrations of fluid boron treatment significantly inhibited flower bud abscission. At 3 days of treatment, the effect of inhibiting flower bud abscission was not obvious compared with the CK group. By 6 days of treatment, both the E2 and E3 treatments showed significant inhibitory effects. By 10 days of treatment, the E2 treatment showed the most significant effect in inhibiting flower bud abscission, and the plants were growing well. The flowering period of the bougainvillea in this group was significantly prolonged.

[0078] 3.2.3 The effect of K1-K3 (Examples 7-9) on inhibiting the shedding of bougainvillea flower buds K1 (1 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), K2 (2 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), and K3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 10As shown in Fig. 2a, the bud drop rate of A-grade flower buds treated with different concentrations of KH2PO4 showed a trend of first decreasing and then increasing with the increase of KH2PO4 concentration. The bud drop rate of A-grade flower buds treated with K2 was the lowest, only 6.5%, which was significantly lower than that of CK group and the other two concentration groups. The bud drop rate of A-grade flower buds treated with K2 was 10.7% lower than that of CK group, and the effect of inhibiting the drop of A-grade flower buds was significant. The bud drop rates of flower buds treated with K1 and K3 were also lower than that of CK group, but the inhibitory effect was not as significant as that of K2 treatment. The bud drop rate of K2 group was high. Figure 10 As shown in Fig. 2b, with the increase of KH2PO4 concentration, the bud drop rate of B-grade flower buds showed a trend of gradually decreasing, and the bud drop rates of flower buds treated with three concentrations were all lower than that of CK group. Among them, the bud drop rate of flower buds treated with K3 was 16.6%, which was 18.7% lower than that of CK group, and the effect of inhibiting the drop of B-grade flower buds was significant. Figure 10 As shown in Fig. 2c, with the increase of KH2PO4 concentration gradient, the bud drop rate of C-grade flower buds showed a trend of gradually decreasing. Among them, the bud drop rate of flower buds treated with K1 was 55%, which was close to the bud drop rate of flower buds treated with CK group, and had no obvious inhibitory effect on the drop of B-grade flower buds. The bud drop rate of flower buds treated with K3 was 23.2%, which was 31.1% lower than that of CK group, and the inhibitory effect was significant. Figure 10 As shown in Fig. 2d, with the increase of KH2PO4 concentration gradient, the bud drop rate of D-grade flower buds gradually decreased, and the bud drop rates of flower buds treated with three concentrations were all significantly lower than that of CK group. Among them, the bud drop rate of flower buds treated with K3 was 39.3%, which was significantly lower than that of CK group 50.3%, and had a significant inhibitory effect on the drop of D-grade flower buds.

[0079] Referring to Fig. 3, Figure 11 Compared with CK group, the three groups of different concentrations of KH2PO4 treatment inhibited the drop of flower buds at 6d, but the inhibitory effect of each group on the drop of flower buds at 10d was not as significant as that at 6d. Among them, K3 treatment had the most significant inhibitory effect on the drop of flower buds, and had inhibitory effect on the drop of B, C and D stage flower buds. K1 treatment had more significant inhibitory effect on the drop of D-grade flower buds, and K2 treatment had more significant inhibitory effect on the drop of A-grade flower buds, so K3 could most significantly inhibit the drop of flower buds.

[0080] In summary, the most suitable concentration of each group of treatment which could significantly inhibit the drop of flower buds was combined as follows: E2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L calcium sugar alcohol), N3 (25 mg / L NAA + 50 mg / L calcium sugar alcohol) and K3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L calcium sugar alcohol), as shown in Fig. 4.Figure 12 As shown, under natural conditions, with the transition of the flowering period from the initial flowering stage to the flower falling stage, the bud abscission rate increased accordingly. E2, N3 and K3 treatments all inhibited the abscission of flower buds compared with the CK group. Among them, the E2 treatment had the best effect on inhibiting the abscission of flower buds at each stage.

[0081] Examples 10-21

[0082] Preparation method: Prepare 1 mg / mL NAA stock solution: weigh 0.1 g NAA and dissolve in 100 ml distilled water, store at 4°C; prepare 1 mg / mL calcium glycerophosphate stock solution: weigh 0.1 g calcium glycerophosphate and dissolve in 100 ml distilled water, store at 4°C; Prepare 1 mg / mL KH2PO4 stock solution: weigh 0.1 g KH2PO4 and dissolve in 100 ml distilled water, store at 4°C; Prepare 1 mg / mL fluid boron stock solution: weigh 0.1 g fluid boron and dissolve in 100 ml distilled water, store at 4°C; Prepare different concentrations of the regulator by using the above stock solutions; The regulator 1 mg / L KH2PO4 of Example 10 refers to 1 mg KH2PO4 in 1 L distilled water.

[0083] The regulator 2 mg / L KH2PO4 of Example 11 refers to 1 mg KH2PO4 in 1 L distilled water.

[0084] The regulator 3 mg / L KH2PO4 of Example 12 refers to 1 mg KH2PO4 in 1 L distilled water.

[0085] The regulator 1 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L calcium glycerophosphate of Example 13 refers to 1 mg KH2PO4, 25 mg NAA and 50 mg calcium glycerophosphate in 1 L distilled water.

[0086] The regulator 2 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L of Example 14 refers to 2 mg KH2PO4, 25 mg NAA and 50 mg calcium glycerophosphate in 1 L distilled water.

[0087] The regulator 3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L calcium glycerophosphate of Example 15 refers to 3 mg KH2PO4, 25 mg NAA and 50 mg calcium glycerophosphate in 1 L distilled water.

[0088] The 0.1 mg / L fluid boron regulator in Example 16 refers to 0.1 mg of fluid boron in 1 L of distilled water.

[0089] The regulator in Example 17, 0.2 mg / L fluid boron, refers to 0.2 mg of fluid boron per 1 L of distilled water.

[0090] The regulator in Example 18, 0.3 mg / L fluid boron, refers to 0.3 mg of fluid boron per 1 L of distilled water.

[0091] The regulator in Example 19, 0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium, refers to 1 L of distilled water containing 0.1 mg fluid boron, 25 mg NAA and 50 mg sugar alcohol calcium.

[0092] The regulator in Example 20, 0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium, refers to 1 L of distilled water containing 0.2 mg fluid boron, 25 mg NAA and 50 mg sugar alcohol calcium.

[0093] The regulator in Example 21, 0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium, refers to 1 L of distilled water containing 0.1 mg fluid boron, 25 mg NAA and 50 mg sugar alcohol calcium.

[0094] Experimental Example 2 - Simulated Storage and Transportation Conditions 1. Plant material: Same as in Experiment 1 2. Test Methods 2.1 Experimental treatment under simulated storage and transportation conditions A randomized block design was used to compare the effects of Examples 10-21 on flowering and bud drop of potted Bougainvillea 'Miss Manila' under simulated storage and transportation conditions. Water spraying (CK) served as the control. 0.1% Tween 20 was added as a surfactant to all treatments. Five pots were treated per group, with three replicates. Spraying was conducted at the initial flowering stage of the plants, on sunny afternoons around 16:00 to avoid the influence of rainy days and high temperatures on the results. After the sprayed plants dried, they were moved into a dark room, where the temperature was maintained at approximately 25°C and the average relative humidity at 72%-80% for 3 days to simulate transportation. After the dark treatment, the plants were moved out and placed under natural conditions with an average of 11 hours of sunlight per day. During the treatment period, routine maintenance was used; watering was done when the surface of the potting soil was dry, ensuring thorough watering until water drained from the bottom of the pot.

[0095] 2.2 Physiological index measurement: consistent with Experimental Example 1 2.3 Data processing: consistent with Experiment 1 3. Data Results 3.1 Effects of different treatments on bougainvillea flowering under simulated storage and transportation conditions 3.1.1 Effects of A1-A3 (Examples 10-12) on Bougainvillea flowering Three groups: A1 (1 mg / L KH2PO4), A2 (2 mg / L KH2PO4), and A3 (3 mg / L KH2PO4), as follows: Figure 13 As shown in Figure a, after 3 days of treatment, the flowering rate of group A3 was 54.5%, close to that of groups A1 and A2 (both around 50%), and higher than the 42.9% flowering rate of group CK. Figure 13 As shown in b, after 6 days of treatment, there was no significant difference between the three treatment groups and the control group, with flowering rates ranging from 81% to 86%. The flowering rate of group A3 was slightly higher than that of the other groups. The flowering rates of different concentrations of KH2PO4 were slightly higher than those of the control group, but there was no significant difference among the three groups with different concentrations of KH2PO4.

[0096] 3.1.2 Effects of D1-D3 (Examples 13-15) on Bougainvillea flowering D1 (1 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), D2 (2 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), D3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 14 As shown in Figure a, after 3 days of treatment, the flowering rate initially decreased and then increased with increasing KH₂PO₄ concentration. The flowering rates of treatments D1 and D3 were higher than those of the control group (CK). Treatment D1 had the highest flowering rate at 49.7%, while treatment D2 had the lowest at 30.5%, which was 9.4% lower than the CK treatment and 16.1% lower than the D1 treatment, significantly inhibiting true flower opening. Figure 14 As shown in b, after 6 days of treatment, the flowering rate of group D3 was the highest at 87.7%, which was 6.1% higher than that of group CK. The flowering rates of groups D1 and D2 were both lower than that of group CK. Among them, the flowering rate of group D2 was the lowest at 69.2%, which was 12.4% lower than that of group CK and 18.5% lower than that of group D3, significantly inhibiting flowering.

[0097] 3.1.3 Effects of B1-B3 (Examples 16-18) on Bougainvillea flowering B1 (0.1 mg / L fluid boron), B2 (0.2 mg / L fluid boron), and B3 (0.3 mg / L fluid boron), such as Figure 15As shown in a of FIG. 6, after 3 days of treatment, the flower opening rate of the B3 group was 46.7%, which was higher than that of the other groups and could significantly promote flower opening. However, the B1 and B2 groups had no obvious promoting effect, and the flower opening rate of the B1 group was 30.6%, which was 12.3% lower than that of the CK group, thereby inhibiting flower opening. As shown in b of FIG. 6, after 6 days of treatment, the flower opening rate showed an upward trend with the increase of the fluid boron concentration. However, only the B3 group could promote the opening of the florets. The flower opening rate of the B3 group was 84.3%, which was higher than that of the B1 and B2 groups. However, there was no significant difference in the flower opening rate between the B3 group and the CK group, which was slightly higher than that of the CK group by 2.7%, and had no significant promoting effect on flower opening. The flower opening rates of the B1 and B2 groups were lower than that of the CK group, and the B1 group had the most obvious inhibitory effect on flower opening, with a flower opening rate of only 61.2%, which was 13.4% lower than that of the CK group. Figure 15 As shown in a of FIG. 6, after 3 days of treatment, the flower opening rate of the B3 group was 46.7%, which was higher than that of the other groups and could significantly promote flower opening. However, the B1 and B2 groups had no obvious promoting effect, and the flower opening rate of the B1 group was 30.6%, which was 12.3% lower than that of the CK group, thereby inhibiting flower opening. As shown in b of FIG. 6, after 6 days of treatment, the flower opening rate showed an upward trend with the increase of the fluid boron concentration. However, only the B3 group could promote the opening of the florets. The flower opening rate of the B3 group was 84.3%, which was higher than that of the B1 and B2 groups. However, there was no significant difference in the flower opening rate between the B3 group and the CK group, which was slightly higher than that of the CK group by 2.7%, and had no significant promoting effect on flower opening. The flower opening rates of the B1 and B2 groups were lower than that of the CK group, and the B1 group had the most obvious inhibitory effect on flower opening, with a flower opening rate of only 61.2%, which was 13.4% lower than that of the CK group.

[0098] 3.1.4 Effects of C1-C3 (Examples 19-21) on the Flowering of Bougainvillea C1 (0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), C2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), and C3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), as shown in a of FIG. 7. Figure 16 As shown in a of FIG. 7, after 3 days of treatment, the flower opening rates of the C2 and C3 groups and the CK group had no significant difference, which were 41%-42%. However, the flower opening rate of the C1 group was 19.5%, which was significantly lower than that of the other groups and obviously inhibited flower opening. As shown in b of FIG. 7, after 6 days of treatment, the flower opening rate of the C3 group was 80.9%, which was close to that of the CK group (81.6%). However, the flower opening rates of the C1 and C2 groups were lower than that of the CK group, in which the flower opening rate of the C2 group was 71%, and the flower opening rate of the C1 group was only 49.7%, which was 31.9% lower than that of the CK group, thereby significantly inhibiting flower opening. Figure 16 As shown in a of FIG. 7, after 3 days of treatment, the flower opening rates of the C2 and C3 groups and the CK group had no significant difference, which were 41%-42%. However, the flower opening rate of the C1 group was 19.5%, which was significantly lower than that of the other groups and obviously inhibited flower opening. As shown in b of FIG. 7, after 6 days of treatment, the flower opening rate of the C3 group was 80.9%, which was close to that of the CK group (81.6%). However, the flower opening rates of the C1 and C2 groups were lower than that of the CK group, in which the flower opening rate of the C2 group was 71%, and the flower opening rate of the C1 group was only 49.7%, which was 31.9% lower than that of the CK group, thereby significantly inhibiting flower opening.

[0099] In summary, under the simulated storage and transportation dark conditions, the most suitable concentrations in the groups that could significantly promote the flowering of Bougainvillea were combined as follows: C3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), A3 (3 mg / L KH2PO4), and D3 (3 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L sugar alcohol calcium). As shown in a of FIG. 8. Figure 17As shown in a of FIG. 6, when 3d was treated, the A3 group in each group had the highest flowering rate of 54.5%, which was 11.6% higher than that of the CK group, significantly promoting flowering. The flowering rate of the C3 group after treatment was 41.1%, which was close to that of the CK group, and had no obvious promoting effect on flowering. As shown in b of FIG. 6, when 6d was treated, the flowering rate of the D3 group was 87.7%, which was 6.1% higher than that of the CK group, but the flowering rates after treatment of each group were not significantly different, and were between 81% and 88%, and only the flowering rate of the C3 group was slightly lower than that of the CK group. Figure 17

[0100] 3.2 Effect of different treatments on inhibition of flower bud abscission of Bougainvillea spectabilis under simulated storage and transportation conditions 3.2.1 Effect of A1-A3 (Example 10-Example 12) on inhibition of flower bud abscission of Bougainvillea spectabilis The three groups A1 (1 mg / L KH2PO4), A2 (2 mg / L KH2PO4), and A3 (3 mg / L KH2PO4) were as shown in FIG. 5. Figure 18 As shown in a of FIG. 6, when 3d was treated, the A3 group in each group had the highest flowering rate of 54.5%, which was 11.6% higher than that of the CK group, significantly promoting flowering. The flowering rate of the C3 group after treatment was 41.1%, which was close to that of the CK group, and had no obvious promoting effect on flowering. As shown in b of FIG. 6, when 6d was treated, the flowering rate of the D3 group was 87.7%, which was 6.1% higher than that of the CK group, but the flowering rates after treatment of each group were not significantly different, and were between 81% and 88%, and only the flowering rate of the C3 group was slightly lower than that of the CK group. Figure 18 As shown in b of FIG. 6, with the increase of the concentration of KH2PO4, the abscission rate first decreased and then increased. The abscission rates of the B-level flower buds treated by the three groups of KH2PO4 were significantly lower than that of the CK group. Among them, the abscission rate of the A2 group was 17.6%, which was 25.8% lower than that of the CK group, and significantly inhibited the abscission of the B-level flower buds. Figure 18 As shown in c of FIG. 6, with the increase of the concentration of KH2PO4, the abscission rate of the C-level flower buds gradually decreased, and the abscission rates of the flower buds treated by the three groups of different concentrations of KH2PO4 were significantly lower than that of the CK group. Among them, the abscission rate of the flower buds treated by the A3 group was 21%, which was 38.2% lower than that of the CK group, and had the most significant effect on inhibiting the abscission of the C-level flower buds. Figure 18 As shown in d of FIG. 6, the abscission rate of the D-level flower buds gradually decreased with the increase of the concentration of KH2PO4, and the A3 group had the most significant effect on inhibiting the abscission, and the abscission rate of the flower buds was 61.8%, which was 25.8% lower than that of the CK group.

[0101] Figure 19 ​It can be seen that compared with the CK group, three groups of different concentrations of KH2PO4 can play a role in inhibiting the shedding of flower buds. In the treatment of 3d, the A2 group treatment can more significantly inhibit the shedding of A and B grade flower buds in the initial flowering stage and the initial flowering stage, and the A3 group treatment can more significantly inhibit the shedding of C and D grade flower buds in the full flowering stage and the flowering stage.

[0102] 3.2.2 D1-D3 (Example 13-Example 15) on inhibiting the shedding of Bougainvillea flower buds D1 (1mg / L KH2PO4+25mg / L NAA+50mg / L sugar alcohol calcium), D2 (2mg / L KH2PO4+25mg / L NAA+50mg / L sugar alcohol calcium), D3 (3mg / L KH2PO4+25mg / L NAA+50mg / L sugar alcohol calcium), as shown in a of Figure 20 , the shedding rate of flower buds treated by D2 and D3 groups is similar, which is significantly lower than that of the CK group, and is 7.5% lower than that of the CK group, but the shedding rate of flower buds treated by D1 group is close to that of the CK group, and has no significant inhibitory effect on the shedding of A grade flower buds. As shown in b of Figure 20 , the shedding rate of flower buds treated by D2 and D3 groups is similar, which is significantly lower than that of the CK group, and is 7.5% lower than that of the CK group, but the shedding rate of flower buds treated by D1 group is close to that of the CK group, and has no significant inhibitory effect on the shedding of A grade flower buds. As shown in b of Figure 20 , the shedding rate of flower buds treated by D2 and D3 groups is similar, which is significantly lower than that of the CK group, and is 7.5% lower than that of the CK group, but the shedding rate of flower buds treated by D1 group is close to that of the CK group, and has no significant inhibitory effect on the shedding of A grade flower buds. As shown in b of Figure 20 , the shedding rate of flower buds treated by D2 and D3 groups is similar, which is significantly lower than that of the CK group, and is 7.5% lower than that of the CK group, but the shedding rate of flower buds treated by D1 group is close to that of the CK group, and has no significant inhibitory effect on the shedding of A grade flower buds. As shown in b of

[0103] Figure 21It can be seen that the treatments D1, D2 and D3 have significant inhibitory effect on bract abscission compared with the CK treatment, and the three treatments have inhibitory effect on bract abscission at all levels. Among them, the inhibitory effect of D2 and D3 on bract abscission at all levels is more significant than that of D1, and the inhibitory effect of D2 and D3 is similar. However, the inhibitory effect of the three treatments on A and D level bract abscission at initial flowering and flower shedding stage is not significant, and there is no significant difference with the CK treatment. The inhibitory effect on B and C level bract abscission at initial flowering and full flowering stage is more significant.

[0104] 3.2.3 Effect of B1-B3 (Example 16-Example 18) on inhibiting bract abscission of Bougainvillea spectabilis B1 (0.1 mg / L fluid boron), B2 (0.2 mg / L fluid boron) and B3 (0.3 mg / L fluid boron), as shown in a of Figure 22 , the abscission rate of A level bract after treatment with different concentrations of fluid boron first increases and then decreases with the increase of concentration. The abscission rate of bract after B3 treatment is 9.4%, which is the lowest among the three treatments, which is 5.8% lower than that of the CK treatment, as shown in the b graph of Figure 22 , the abscission rate of B level bract among the three concentration fluid boron treatments is not significant, which is 15%-21%, which is 43.4% lower than that of the CK treatment. Among them, 0.2 mg·L -1 The inhibitory effect of fluid boron treatment on B level bract abscission is the most significant, which is 15.1%, which is 28.3% lower than that of the CK treatment. As shown in the c graph of Figure 22 , the abscission rate of C level bract after treatment with three concentrations of fluid boron is significantly lower than that of the CK treatment, and the abscission rate gradually decreases with the increase of fluid boron treatment concentration. Among them, the inhibitory effect of B3 treatment on bract abscission is more significant, which is 32.7% lower than that of the CK treatment. As shown in the d graph of Figure 22 , among the D level bracts treated with different concentrations of fluid boron, the abscission rate of bract treated with B1 and B2 is not significantly different from that of the CK treatment, and has no obvious inhibitory effect on bract abscission. Among the three concentration gradient treatments, the abscission rate of bract treated with B3 is 59.6%, which is significantly lower than that of the CK treatment (87.8%), and can significantly inhibit bract abscission.

[0105] From Figure 23It can be seen that treatments B1, B2, and B3 all inhibited the abscission of flower buds at all levels compared with the control group, and the inhibitory effect was evident in all three treatments after 3 days of treatment. In the A and B grade flower buds (from the initial flowering stage to the early flowering stage), there was no significant difference in the inhibitory effect of the three concentration treatments on the abscission of A and B grade flower buds. In the C and D grade flower buds (from the full bloom stage to the flower fall stage), the abscission of flower buds treated with group B3 was significantly reduced compared with the control group, demonstrating a significant inhibitory effect on flower bud abscission.

[0106] 3.2.4 Effect of C1-C3 (Examples 19-21) on inhibiting bougainvillea flower bud drop C1 (0.1 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), C2 (0.2 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), and C3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L sugar alcohol calcium), such as Figure 24 As shown in a, among the grade A flower buds after each treatment, the abscission rate of groups C1 and C2 was slightly lower than that of group CK, but the difference was not significant. The abscission rate of group C3 was 8.2%, significantly lower than that of group CK, indicating a significant inhibitory effect on the abscission of grade A flower buds. Figure 24 As shown in b, with the increase of fluid boron concentration in the treatment combination, the abscission rate of grade B flower buds gradually decreased. The abscission rate of flower buds after the three treatment groups was significantly lower than that of the CK group. The C3 group was 14%, which was 29.6% lower than the CK group, and had the most significant effect in inhibiting the abscission of grade B flower buds. Figure 24 As shown in Figure c, the abscission rate of grade C buds gradually decreased with increasing boron concentration in the treatment combination, and all three treatments were significantly lower than the CK group. Specifically, group C3 had a rate of 27.5%, 31.7% lower than the CK group, showing the most significant inhibitory effect on grade C bud abscission among the three treatments. As shown in Figure 24, the abscission rate of grade D buds initially increased and then decreased with increasing boron concentration in the treatment combination. Groups C1 and C3 had significantly lower abscission rates than the CK group, but the abscission rates were similar between groups C1 and C3, indicating no significant difference in their inhibitory effect on grade D bud abscission.

[0107] from Figure 25 It can be seen that, compared with the CK group, all three treatments inhibited the abscission of flower buds at all levels. The C3 group treatment showed a significant inhibitory effect on the abscission of flower buds at all levels from the initial flowering stage to the flowering stage, making it the best treatment combination for inhibiting the abscission of bougainvillea flower buds.

[0108] In summary, the most suitable concentrations of each treatment group that can significantly inhibit the bud abscission under the simulated storage and transportation dark conditions were combined as follows: C3 (0.3 mg / L fluid boron + 25 mg / L NAA + 50 mg / L calcium sugar alcohol), A2 (2 mg / L KH2PO4), and D2 (2 mg / L KH2PO4 + 25 mg / L NAA + 50 mg / L calcium sugar alcohol). As shown in Figure 26 As shown in FIG. 5, after the simulated storage and transportation treatment, the abscission rate of each level of flower bud gradually increased as the process of the initial flowering stage to the flower falling stage, and the abscission rate of each treatment group was lower than that of the CK group. In the A level flower bud, the C3 and D2 treatments had the most significant inhibitory effect on the flower bud abscission; in the B level flower bud, the C3 treatment had the lowest flower bud abscission rate; in the C level flower bud, the D2 treatment had the lowest flower bud abscission rate; and in the D level flower bud, the B3 treatment had the lowest flower bud abscission rate. In the A, B, and C level flower buds in the initial flowering stage to the full flowering stage, the C3 and D2 treatments of different plant growth regulator combinations had a more significant inhibitory effect on the abscission, while in the D level flower bud in the flower falling stage, the single B3 treatment was the best treatment for inhibiting the flower bud abscission.

[0109] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A regulator for promoting flowering of bougainvillea or inhibiting bud drop, characterized in that, The regulator promotes flowering of bougainvillea or inhibits bud drop under natural or simulated storage and transportation conditions. The regulator that promotes bougainvillea flowering under natural conditions consists of 0.2 mg / L fluid boron, 25 mg / L NAA and 50 mg / L sugar alcohol calcium; The regulators that inhibit flower bud abscission under natural conditions are 25 mg / L NAA and 50 mg / L sugar alcohol calcium; The regulator that promotes bougainvillea flowering under simulated storage and transportation conditions is 2 mg / L KH2PO4; The regulator used to inhibit bud drop under simulated storage and transportation conditions is 0.3 mg / L fluid boron.

2. The regulator for promoting flowering or inhibiting bud drop in bougainvillea as described in claim 1, characterized in that, The natural conditions are: temperature 22℃-26℃, sunshine duration 10-12h, and relative humidity 74%-82%.

3. The regulator for promoting flowering or inhibiting bud drop in bougainvillea as described in claim 1, characterized in that, The simulated storage and transportation conditions are as follows: after the plants to be sprayed are dried, they are moved into a dark room and the temperature is maintained at 24℃-26℃ and the relative humidity is 72%-80%. The simulated transportation lasts for 2-4 days. After the dark treatment is completed, the plants are placed under natural conditions with 10-12 hours of sunlight.

4. A regulator for promoting flowering or inhibiting bud drop in bougainvillea as described in claim 1, characterized in that, Spray bougainvillea with external spray until water droplets fall from the plant's leaves and flower buds.

5. A regulator for promoting flowering or inhibiting bud drop in bougainvillea as described in claim 4, characterized in that, Before spraying, the regulator needs to be mixed with 0.1% Tween 20.

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