A carbon-based biological liquid organic water-soluble fertilizer as well as a preparation method and application thereof

Abstract

This invention discloses a carbon-based biological liquid organic water-soluble fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The water-soluble fertilizer of this invention comprises the following raw materials in parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial inoculant, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to a total of 100 parts. This invention screens three strains of Pseudomonas sevivar, Bacillus microepiploicus, and Bacillus thuringiensis, which function in three dimensions: promoting growth, improving the rhizosphere environment, and preventing and controlling pests and diseases. These strains are combined with organic components such as seaweed extract and potassium humate in fertilizers, as well as other components, to form a comprehensive system of "nutrition + growth promotion + disease resistance + quality improvement", which significantly improves the yield and quality of grapes.

Description

Technical Field

[0001] This invention belongs to the field of bio-organic fertilizer technology, specifically relating to a carbon-based bio-liquid organic water-soluble fertilizer, its preparation method, and its application. Background Technology

[0002] Grapes (Vitis vinifera L.) are one of the world's most widely cultivated fruit crops, prized for their excellent taste and popularity among consumers. Rich in various nutrients such as sugars, acids, vitamins, minerals, polyphenols, dietary fiber, and amino acids, grapes are highly beneficial to human health, helping to lower LDL cholesterol and reduce the risk of heart disease and cancer. Grapes have a wide range of applications, including fresh consumption, juicing, winemaking, and dried grape products, playing a vital role in promoting agricultural economic development.

[0003] To increase grape yields, farmers have used large amounts of chemical fertilizers during production, leading to soil acidification, compaction, and nutrient imbalances. This severely reduces soil fertility and health, resulting in decreased fruit yield and quality, and seriously hindering sustainable agricultural development. To address the ecological and quality safety issues caused by the overuse of chemical fertilizers, water-soluble fertilizers are favored due to their comprehensive nutrient content, high absorption efficiency, and suitability for modern fertilization methods such as drip irrigation. Meanwhile, "carbon-based" fertilizers containing organic matter such as seaweed extracts and potassium humate can replenish soil organic matter, improve soil structure, and provide more comprehensive nutrition. Furthermore, microbial inoculants have shown great potential in agriculture. Their multiple functions, including nitrogen fixation, phosphorus and potassium solubilization, secretion of growth stimulants, antagonism of pathogens, and induction of plant systemic resistance, offer new solutions for building healthy and sustainable agricultural production systems.

[0004] However, many bio-fertilizer products on the market currently suffer from problems such as limited functionality, unscientific strain formulation, or unstable activity. Single strains often only address one specific issue and are insufficient to address the multiple complex challenges faced by grape growers, including nutrition, soil environment, and pests and diseases. Therefore, developing a compound bio-liquid water-soluble fertilizer that integrates nutrient supply, soil improvement, disease control, and quality enhancement, through the scientific formulation of various functional microorganisms and organic and inorganic nutrients to form a synergistic and comprehensive management system, is urgently needed and has significant application value for promoting the green, high-quality, and efficient development of the grape industry. Summary of the Invention

[0005] The purpose of this invention is to provide a carbon-based biological liquid organic water-soluble fertilizer for grape cultivation, which significantly improves grape yield and quality.

[0006] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0007] A carbon-based biological liquid organic water-soluble fertilizer comprises the following raw materials in parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial agent, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to make up to 100 parts.

[0008] Furthermore, the compound microbial agent includes *Pseudomonas sevivar*, *Bacillus microglobulus*, and *Bacillus thuringiensis*.

[0009] Furthermore, the *Pseudomonas sevivar* strain was purchased from the China Microbial Culture Collection Center (CGMCC), strain number CGMCC 1.12351, with an original preservation date of October 30, 2012; the *Bacillus microphaga-Oceanopsis* strain was purchased from the China Microbial Culture Collection Center (CGMCC), strain number CGMCC 1.12636, with an original preservation date of June 27, 2013; and the *Bacillus thuringiensis* strain was purchased from the China Microbial Culture Collection Center (CGMCC), strain number CGMCC 1.6576, with an original preservation date of January 4, 2007.

[0010] Furthermore, the preparation method of the compound microbial agent is as follows:

[0011] (1) After thawing Bacillus microepiploicus, activate it in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and incubate at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture. The seed culture was inoculated into an LB liquid fermenter at a rate of 1% and cultured at 30℃ and 200 r / min. The viable cell count of the culture was measured and found to be 1×10. 8 The culture was terminated after cfu / mL, and the Bacillus microepiphyticus bacterial suspension was obtained.

[0012] (2) Bacillus thuringiensis and Pseudomonas sevivar were activated in nutrient gravy agar medium, and single colonies were picked and inoculated into nutrient gravy liquid medium and cultured at 30°C and 180 r / min until OD. 600 =0.6 to obtain seed liquid. The seed liquid was inoculated into nutrient gravy liquid fermentation tanks at a rate of 1% and cultured at 30℃ and 180r / min. The viable count was measured and reached 1×10 8 The culture was terminated after cfu / mL, and Bacillus thuringiensis and Pseudomonas sevivar were obtained respectively.

[0013] (3) The three bacterial solutions prepared above are mixed in a volume ratio of 1:1:1 and then freeze-dried into freeze-dried powder to obtain a composite microbial agent.

[0014] Furthermore, the trace element-containing substances include 0.3-0.5 parts of EDTA-Fe, 0.2-0.3 parts of EDTA-Mn, 0.2-0.3 parts of EDTA-Zn, 0.2-0.3 parts of boric acid, and 0.1-0.2 parts of ammonium molybdate.

[0015] Furthermore, the organosilicon surfactant is a polyether-modified trisiloxane.

[0016] A method for preparing a carbon-based biological liquid organic water-soluble fertilizer includes the following steps:

[0017] First, dissolve the substances containing trace elements, then add seaweed extract, potassium humate, potassium dihydrogen phosphate, potassium nitrate, urea, calcium nitrate, and magnesium sulfate heptahydrate to water in sequence. After mixing and stirring the above substances evenly, add sodium dodecylbenzenesulfonate, potassium sorbate, and organosilicon surfactant and mix evenly. Finally, add compound microbial inoculant to obtain the final product, carbon-based biological liquid organic water-soluble fertilizer.

[0018] The present invention also provides the application of the aforementioned carbon-based biological liquid organic water-soluble fertilizer, which is used in grape cultivation to improve grape yield and quality.

[0019] The carbon-based biological liquid organic water-soluble fertilizer of this invention is used after being diluted 500 times.

[0020] This invention selects three functional microbial strains: *Pseudomonas sevivar*, *Bacillus microglobulus*, and *Bacillus thuringiensis*. *Pseudomonas sevivar* can fix atmospheric nitrogen into ammonia, providing an additional nitrogen source for grapes. Simultaneously, it secretes organic acids to dissolve fixed phosphorus and potassium elements in the soil, significantly improving the utilization rate of nutrients such as potassium dihydrogen phosphate and potassium nitrate in fertilizers. This directly promotes the vegetative and reproductive growth of grapes, laying the foundation for high yields. Furthermore, it secretes the plant hormone IAA, directly stimulating grape root development and cell division, forming larger and stronger root systems, enhancing water and nutrient absorption capacity, thereby increasing fruit set rate and fruit enlargement speed. At the same time, robust plants ensure the accumulation of more dry matter in the fruit. *Bacillus microglobulus* has strong environmental adaptability and metabolic capacity; its metabolites contribute to the formation of soil aggregates, alleviating compaction and allowing for smoother root respiration and a more optimal growing environment. It can also secrete cellulase, accelerating the decomposition of soil organic matter and converting large molecules in seaweed extracts in fertilizers into smaller molecules that are more easily absorbed by plants, thus improving fertilizer utilization. When pathogens attack, it activates the grape's own defense system, allowing the vine to react faster and stronger, reducing the impact of diseases on yield. Bacillus thuringiensis can effectively control lepidopteran pests on grapes, directly protecting the leaves and fruit of the vine, preventing leaf and fruit drop and fruit borer damage caused by pests, thereby ensuring and increasing yield. In addition, it directly eliminates physical damage to the fruit caused by pests, greatly improving the commercial appearance of the fruit, reducing the use of chemical pesticides, lowering the risk of pesticide residues, and better meeting the production requirements of green and organic food, thereby enhancing the market value and safety of grapes.

[0021] The three bacteria work synergistically to promote plant health, enabling the plant to perform more efficient photosynthesis, synthesize more sugars and transport them to the fruit, directly increasing the soluble solids content of grapes. Reducing diseases means reducing pesticide use, resulting in safer and better-quality fruit.

[0022] Beneficial effects

[0023] This invention screens three strains of Pseudomonas sevivar, Bacillus microepiploicus, and Bacillus thuringiensis, which function in three key dimensions: promoting growth, improving the rhizosphere environment, and preventing and controlling pests and diseases. These strains are combined with organic components such as seaweed extract and potassium humate in fertilizers, as well as macro- and micro-elements, to form a comprehensive system of "nutrition + growth promotion + disease resistance + quality improvement." The components promote each other and significantly improve the yield and quality of grapes. Detailed Implementation

[0024] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.

[0025] Example 1

[0026] A carbon-based biological liquid organic water-soluble fertilizer comprises the following raw materials in parts by weight: 15 parts seaweed extract, 10 parts potassium humate, 5 parts compound microbial agent, 3 parts potassium dihydrogen phosphate, 2 parts potassium nitrate, 2 parts urea, 2 parts calcium nitrate, 1 part magnesium sulfate heptahydrate, 1 part trace element substance, 1 part sodium dodecylbenzenesulfonate, 0.5 parts potassium sorbate, 0.5 parts organosilicon surfactant, and water to make up to 100 parts.

[0027] The compound microbial agent includes *Pseudomonas sevivar*, *Bacillus microelandii*, and *Bacillus thuringiensis*.

[0028] The *Pseudomonas sevivar* strain is numbered CGMCC 1.12351; the *Bacillus microepiploicus* strain is numbered CGMCC 1.12636; and the *Bacillus thuringiensis* strain is numbered CGMCC 1.6576.

[0029] The preparation method of the compound microbial agent is as follows:

[0030] (1) After thawing Bacillus microepiploicus, activate it in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and incubate at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture. The seed culture was inoculated into an LB liquid fermenter at a rate of 1% and cultured at 30℃ and 200 r / min. The viable cell count of the culture was measured and found to be 1×10. 8 The culture was terminated after cfu / mL, and the Bacillus microepiphyticus bacterial suspension was obtained.

[0031] (2) Bacillus thuringiensis and Pseudomonas sevivar were activated in nutrient gravy agar medium, and single colonies were picked and inoculated into nutrient gravy liquid medium and cultured at 30°C and 180 r / min until OD. 600 =0.6 to obtain seed liquid. The seed liquid was inoculated into nutrient gravy liquid fermentation tanks at a rate of 1% and cultured at 30℃ and 180r / min. The viable count was measured and reached 1×10 8 The culture was terminated after cfu / mL, and Bacillus thuringiensis and Pseudomonas sevivar were obtained respectively.

[0032] (3) The three bacterial solutions prepared above are mixed in a volume ratio of 1:1:1 and then freeze-dried into freeze-dried powder to obtain a composite microbial agent.

[0033] The trace element-containing substances include 0.5 parts EDTA-Fe, 0.3 parts EDTA-Mn, 0.3 parts EDTA-Zn, 0.3 parts boric acid, and 0.2 parts ammonium molybdate.

[0034] The organosilicon surfactant is a polyether-modified trisiloxane.

[0035] A method for preparing a carbon-based biological liquid organic water-soluble fertilizer includes the following steps:

[0036] First, dissolve the substances containing trace elements, then add seaweed extract, potassium humate, potassium dihydrogen phosphate, potassium nitrate, urea, calcium nitrate, and magnesium sulfate heptahydrate to water in sequence. After mixing and stirring the above substances evenly, add sodium dodecylbenzenesulfonate, potassium sorbate, and organosilicon surfactant and mix evenly. Finally, add compound microbial inoculant to obtain the final product, carbon-based biological liquid organic water-soluble fertilizer.

[0037] Example 2

[0038] A carbon-based biological liquid organic water-soluble fertilizer comprises the following raw materials in parts by weight: 20 parts seaweed extract, 12 parts potassium humate, 8 parts compound microbial agent, 4 parts potassium dihydrogen phosphate, 3 parts potassium nitrate, 3 parts urea, 3 parts calcium nitrate, 1 part magnesium sulfate heptahydrate, 1.5 parts trace element substances, 2 parts sodium dodecylbenzenesulfonate, 1 part potassium sorbate, 1 part organosilicon surfactant, and water to make up to 100 parts.

[0039] The compound microbial agent includes *Pseudomonas sevivar*, *Bacillus microelandii*, and *Bacillus thuringiensis*.

[0040] The *Pseudomonas sevivar* strain is numbered CGMCC 1.12351; the *Bacillus microepiploicus* strain is numbered CGMCC 1.12636; and the *Bacillus thuringiensis* strain is numbered CGMCC 1.6576.

[0041] The preparation method of the compound microbial agent is as follows:

[0042] (1) After thawing Bacillus microepiploicus, activate it in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and incubate at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture. The seed culture was inoculated into an LB liquid fermenter at a rate of 1% and cultured at 30℃ and 200 r / min. The viable cell count of the culture was measured and found to be 1×10. 8 The culture was terminated after cfu / mL, and the Bacillus microepiphyticus bacterial suspension was obtained.

[0043] (2) Bacillus thuringiensis and Pseudomonas sevivar were activated in nutrient gravy agar medium, and single colonies were picked and inoculated into nutrient gravy liquid medium and cultured at 30°C and 180 r / min until OD. 600 =0.6 to obtain seed liquid. The seed liquid was inoculated into nutrient gravy liquid fermentation tanks at a rate of 1% and cultured at 30℃ and 180r / min. The viable count was measured and reached 1×10 8The culture was terminated after cfu / mL, and Bacillus thuringiensis and Pseudomonas sevivar were obtained respectively.

[0044] (3) The three bacterial solutions prepared above are mixed in a volume ratio of 1:1:1 and then freeze-dried into freeze-dried powder to obtain a composite microbial agent.

[0045] The trace element-containing substances include 0.4 parts EDTA-Fe, 0.2 parts EDTA-Mn, 0.2 parts EDTA-Zn, 0.2 parts boric acid, and 0.1 parts ammonium molybdate.

[0046] The organosilicon surfactant is a polyether-modified trisiloxane.

[0047] A method for preparing a carbon-based biological liquid organic water-soluble fertilizer includes the following steps:

[0048] First, dissolve the substances containing trace elements, then add seaweed extract, potassium humate, potassium dihydrogen phosphate, potassium nitrate, urea, calcium nitrate, and magnesium sulfate heptahydrate to water in sequence. After mixing and stirring the above substances evenly, add sodium dodecylbenzenesulfonate, potassium sorbate, and organosilicon surfactant and mix evenly. Finally, add compound microbial inoculant to obtain the final product, carbon-based biological liquid organic water-soluble fertilizer.

[0049] Example 3

[0050] A carbon-based biological liquid organic water-soluble fertilizer comprises the following raw materials in parts by weight: 25 parts seaweed extract, 15 parts potassium humate, 10 parts compound microbial agent, 5 parts potassium dihydrogen phosphate, 4 parts potassium nitrate, 3 parts urea, 3 parts calcium nitrate, 2 parts magnesium sulfate heptahydrate, 1.5 parts trace element substances, 2 parts sodium dodecylbenzenesulfonate, 1 part potassium sorbate, 1 part organosilicon surfactant, and water to make up to 100 parts.

[0051] The compound microbial agent includes *Pseudomonas sevivar*, *Bacillus microelandii*, and *Bacillus thuringiensis*.

[0052] The *Pseudomonas sevivar* strain is numbered CGMCC 1.12351; the *Bacillus microepiploicus* strain is numbered CGMCC 1.12636; and the *Bacillus thuringiensis* strain is numbered CGMCC 1.6576.

[0053] The preparation method of the compound microbial agent is as follows:

[0054] (1) After thawing Bacillus microepiploicus, activate it in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and incubate at 30℃ and 200r / min until OD. 600=0.6 to obtain seed culture. The seed culture was inoculated into an LB liquid fermenter at a rate of 1% and cultured at 30℃ and 200 r / min. The viable cell count of the culture was measured and found to be 1×10. 8 The culture was terminated after cfu / mL, and the Bacillus microepiphyticus bacterial suspension was obtained.

[0055] (2) Bacillus thuringiensis and Pseudomonas sevivar were activated in nutrient gravy agar medium, and single colonies were picked and inoculated into nutrient gravy liquid medium and cultured at 30°C and 180 r / min until OD. 600 =0.6 to obtain seed liquid. The seed liquid was inoculated into nutrient gravy liquid fermentation tanks at a rate of 1% and cultured at 30℃ and 180r / min. The viable count was measured and reached 1×10 8 The culture was terminated after cfu / mL, and Bacillus thuringiensis and Pseudomonas sevivar were obtained respectively.

[0056] (3) The three bacterial solutions prepared above are mixed in a volume ratio of 1:1:1 and then freeze-dried into freeze-dried powder to obtain a composite microbial agent.

[0057] The trace element-containing substances include 0.3 parts EDTA-Fe, 0.2 parts EDTA-Mn, 0.2 parts EDTA-Zn, 0.2 parts boric acid, and 0.1 parts ammonium molybdate.

[0058] The organosilicon surfactant is a polyether-modified trisiloxane.

[0059] A method for preparing a carbon-based biological liquid water-soluble fertilizer includes the following steps:

[0060] First, dissolve the substances containing trace elements, then add seaweed extract, potassium humate, potassium dihydrogen phosphate, potassium nitrate, urea, calcium nitrate, and magnesium sulfate heptahydrate to water in sequence. After mixing and stirring the above substances evenly, add sodium dodecylbenzenesulfonate, potassium sorbate, and organosilicon surfactant and mix evenly. Finally, add compound microbial inoculant to obtain the final product, carbon-based biological liquid organic water-soluble fertilizer.

[0061] Comparative Example 1

[0062] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent uses Pseudomonas sevivar and Bacillus microelandii in a volume ratio of 1:1.

[0063] Comparative Example 2

[0064] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent uses Pseudomonas sevivar and Bacillus thuringiensis in a volume ratio of 1:1.

[0065] Comparative Example 3

[0066] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent uses Bacillus microphalaeocarpus and Bacillus thuringiensis in a volume ratio of 1:1.

[0067] Comparative Example 4

[0068] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent only uses Pseudomonas sevivar.

[0069] Comparative Example 5

[0070] Compared with Example 3, this comparative example uses only Bacillus microbial agent, while the other raw materials and steps are the same as in Example 3.

[0071] Comparative Example 6

[0072] Compared with Example 3, this comparative example uses the same raw materials and steps as Example 3, except that the compound microbial agent only uses Bacillus thuringiensis.

[0073] Performance testing

[0074] Planting Trial

[0075] The test material was 5-year-old Ruby grapevines. The soil nutrient content was as follows: organic matter 12.58 g / kg, available nitrogen 63.79 mg / kg, available phosphorus 123.16 mg / kg, and readily available phosphorus 126.92 mg / kg. Ten treatment groups were set up: water-soluble fertilizers prepared in Examples 1-3 and Comparative Examples 1-6 were used respectively, and a blank control (CK group) without fertilizer was set up. The grape planting density was 120 vines per mu (approximately 667 square meters), with 10 vines per treatment plot, 3 replicates, and a randomized block design. Specific application methods for water-soluble fertilizers in each treatment group: Dilute the water-soluble fertilizers in each treatment group 500 times before use; apply the water-soluble fertilizers to the roots before flowering, using 3 kg of the original solution per acre, once every 10 days, for a total of two applications; apply the fertilizers to the leaves during the early fruit enlargement and coloring stages, spraying until water droplets fall from the leaves, once every two weeks, for a total of 4 applications.

[0076] Grape yield: Three vines were randomly selected from each treatment group, and all fruits were harvested at once to calculate the yield per vine and convert it into plot yield.

[0077] Grape quality: Three bunches of grapes were randomly selected from each treatment group for analysis of single fruit weight, single bunch weight, soluble solids content, soluble sugar, titratable acid content, vitamin C content, and fruit coloring. Soluble solids content was determined using a TD-45 digital refractometer, soluble sugar content was determined using the anthrone colorimetric method, titratable acid content was determined using the sodium hydroxide titration method, and vitamin C content was determined using the 2,4-dinitrophenylhydrazine colorimetric method. Fruit coloring was analyzed using a colorimeter.

[0078] The above test data are shown in Tables 1 and 2.

[0079] Table 1. Grape Fruit Appearance Quality Data

[0080]

[0081] As shown in Table 1, compared with the control (CK), the water-soluble fertilizer of Example 3 of this invention increased the weight of a single fruit and the weight of a single bunch by 14.35% and 17.61%, respectively, indicating that the water-soluble fertilizer of this invention can significantly increase the weight of a single grape fruit and the weight of a single bunch. The color difference analyzer was used to analyze the color of the grape fruit. The color brightness was directly proportional to the value; the higher the value, the higher the gloss. A higher redness indicated a more pronounced red hue, and a higher yellowness indicated a more pronounced yellow hue. Compared with the blank control, the water-soluble fertilizers of Examples 1-3 of this invention resulted in higher fruit brightness and redness, indicating that the water-soluble fertilizer of this invention helps to improve the brightness and redness of grape fruit.

[0082] Table 2. Grape Yield and Grape Fruit Internal Quality Data

[0083]

[0084] As shown in Table 2, compared with the control group (CK), the water-soluble fertilizers of Examples 1-3 of this invention significantly improved the soluble solids content and organic acid content of the fruit. Since fruit flavor mainly depends on the soluble solids content and organic acid content, compared with the control group, the water-soluble fertilizers of Examples 1-3 of this invention increased the soluble solids content, reduced the titratable acid content, increased the solids-acid ratio, and improved the fruit flavor. Furthermore, the water-soluble fertilizers of this invention have a significant effect on increasing grape yield. Comparative Examples 1-6, which changed the composition of the microbial inoculant in the water-soluble fertilizer, showed varying degrees of decrease in intrinsic quality and yield compared to Example 3, indicating that the three microbial strains selected in this invention have a synergistic effect; the absence of any one strain weakens the effect.

[0085] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. A carbon-based bio-liquid organic water-soluble fertilizer, characterized in that, The ingredients include the following parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial agent, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to make up to 100 parts. The compound microbial agent includes *Pseudomonas sevivar*, *Bacillus microelandii*, and *Bacillus thuringiensis*. The strain number of *Pseudomonas sevivar* is CGMCC 1.12351; the strain number of *Bacillus microepiploicus* is CGMCC 1.12636; and the strain number of *Bacillus thuringiensis* is CGMCC 1.6576.

2. The carbon-based bio-liquid organic water-soluble fertilizer according to claim 1, characterized in that, The preparation method of the composite microbial agent is as follows: (1) After thawing Bacillus microepiploicus, activate it in LB solid medium, pick a single colony and inoculate it into LB liquid medium, and incubate at 30℃ and 200r / min until OD. 600 =0.6 to obtain seed culture. The seed culture was inoculated into an LB liquid fermenter at a rate of 1% and cultured at 30℃ and 200 r / min. The viable cell count of the culture was measured and found to be 1×10. 8 The culture was terminated after cfu / mL, and the Bacillus microepiphyticus bacterial suspension was obtained. (2) Bacillus thuringiensis and Pseudomonas sevivar were activated in nutrient gravy agar medium, and single colonies were picked and inoculated into nutrient gravy liquid medium and cultured at 30°C and 180 r / min until OD. 600 =0.6 to obtain seed liquid. The seed liquid was inoculated into nutrient gravy liquid fermentation tanks at a rate of 1% and cultured at 30℃ and 180r / min. The viable count was measured and reached 1×10 8 The culture was terminated after cfu / mL, and Bacillus thuringiensis and Pseudomonas sevivar were obtained respectively. (3) The three bacterial solutions prepared above are mixed in a volume ratio of 1:1:1 and then freeze-dried into freeze-dried powder to obtain a composite microbial agent.

3. The carbon-based bio-liquid organic water-soluble fertilizer according to claim 1, characterized in that, The trace element-containing substances include 0.3-0.5 parts of EDTA-Fe, 0.2-0.3 parts of EDTA-Mn, 0.2-0.3 parts of EDTA-Zn, 0.2-0.3 parts of boric acid, and 0.1-0.2 parts of ammonium molybdate.

4. The carbon-based bio-liquid organic water-soluble fertilizer according to claim 1, characterized in that, The organosilicon surfactant is a polyether-modified trisiloxane.

5. A method for preparing the carbon-based biological liquid organic water-soluble fertilizer according to any one of claims 1-4, characterized in that, Includes the following steps: First, dissolve the substances containing trace elements, then add seaweed extract, potassium humate, potassium dihydrogen phosphate, potassium nitrate, urea, calcium nitrate, and magnesium sulfate heptahydrate to water in sequence. After mixing and stirring the above substances evenly, add sodium dodecylbenzenesulfonate, potassium sorbate, and organosilicon surfactant and mix evenly. Finally, add compound microbial inoculant to obtain the final product, carbon-based biological liquid organic water-soluble fertilizer.

6. The use of the carbon-based bio-liquid organic water-soluble fertilizer according to any one of claims 1 to 4, characterized in that, The water-soluble fertilizer is used in grape cultivation to improve grape yield and quality.

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