High-activity tablet candy containing dogwood extract and preparation method of high-activity tablet candy

Abstract

The invention belongs to the technical field of medicinal and edible functional foods, and particularly relates to a high-activity tablet candy containing a dogwood extract and a preparation method of the high-activity tablet candy. The preparation method comprises the following steps: by taking dried cornus officinalis pulp as a main raw material, efficiently enriching iridoid glycoside (loganin and morroniside), polysaccharide and total phenolic acid active ingredients by virtue of an ultrasonic-microwave synergistic extraction and composite enzymolysis coupling process, then optimizing a formula by virtue of functional auxiliary materials, granulating and tabletting. The total amount of loganin and morroniside in the product is larger than or equal to 5%, the total polysaccharide is larger than or equal to 8%, the total phenolic acid is larger than or equal to 3%, the product has the effects of enhancing immunity, resisting oxidation and assisting in bacteriostasis, is moderate in sour and sweet taste and convenient to take, and solves the problems that a traditional dogwood product is low in active ingredient utilization rate, gastrointestinal irritation risks exist, and the dosage form is single. The preparation process is green and efficient, the extraction time is shortened by 40% or above, the yield of the active ingredients is increased by 30%-50% compared with a traditional method, and the method is suitable for industrial production.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology that combines medicine and food, and specifically relates to a highly active compressed candy containing Cornus officinalis extract and its preparation method. Background Technology

[0002] Cornus officinalis (Cornus officinalis Sieb. et Zucc.) is the dried, ripe fruit pulp of a plant in the Cornaceae family, genus Cornus. In 2023, it was included in the national list of substances with both medicinal and edible properties. It possesses effects such as tonifying the liver and kidneys, astringing and consolidating, enhancing immunity, and anti-oxidation. Its main active components are iridoid glycosides (loganin, monoglycoside), polysaccharides, triterpenoids (ursolic acid, oleanolic acid), and total phenolic acids. Among these, iridoid glycosides have anti-inflammatory and neuroprotective effects, polysaccharides can enhance immune function, and total phenolic acids have significant antibacterial activity.

[0003] The existing Cornus officinalis-related products have the following technical defects: (1) The extraction process is simple, and traditional reflux extraction or single ultrasonic extraction is mostly used, resulting in low yield of active ingredients. For example, the yield of iridoid glycosides is only 2.63% to 6.36%, and high temperature or long-term extraction can easily cause component degradation; (2) The product dosage form is limited, and most of them are Chinese medicine decoction pieces, oral liquids or ordinary foods. There is a lack of functional products with high activity, portability and palatability, and some oral drug preparations have gastrointestinal irritation problems; (3) The synergy of efficacy components is insufficient. Existing products mostly focus on the development of single components and do not give full play to the synergistic health care effects of iridoid glycosides, polysaccharides and total phenolic acids.

[0004] Therefore, developing a functional food product made from Cornus officinalis that is efficiently extracted, has synergistic effects with multiple components, and is safe for consumption is of great significance. This issue is also a technical challenge that urgently needs to be solved in the development and production of other products containing medicinal and edible functional ingredients. Summary of the Invention

[0005] In view of the problems and shortcomings of the existing technology, the purpose of this invention is to provide a highly active compressed candy containing Cornus officinalis extract and its preparation method.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: The first aspect of this invention provides a method for extracting Cornus officinalis extract, comprising the following steps: (1) Mix Cornus officinalis powder with water at a ratio of 1g:(15-20)mL to obtain a mixture. Adjust the pH of the mixture to 4.5-5.5. Then add 0.3%-0.8% of the mass of Cornus officinalis powder with a compound enzyme. Enzymatically hydrolyze the mixture at 50-55℃ for 60-90 minutes to obtain an enzyme hydrolysate. Then boil the enzyme hydrolysate to inactivate it. (2) Add ethanol solution to the inactivated enzyme hydrolysate, and then place it in an ultrasonic-microwave synergistic extraction device for extraction. After extraction, filter and collect the Cornus officinalis extract. The Cornus officinalis extract is then concentrated and spray-dried to obtain Cornus officinalis extract.

[0007] Preferably, in step (1), the composite enzyme is a mixture of cellulase and pectinase; wherein the mass ratio of cellulase to pectinase is (1-2):1; and the enzyme activity units are all ≥5000U / g.

[0008] Preferably, in step (2), the extraction conditions are: ultrasonic power of 300-400W, microwave power of 150-200W, extraction temperature of 60-70℃, and extraction time of 40-60min.

[0009] Preferably, in step (2), the volume percentage concentration of the ethanol solution is 60% to 70%, and the volume ratio of the inactivated enzyme hydrolysate to the ethanol solution is 1:(20 to 25).

[0010] Preferably, in step (2), the ultrasonic-microwave synergistic extraction adopts an intermittent operation mode, pausing for 5 minutes after every 15 minutes of extraction, and repeating 2 to 3 times.

[0011] Preferably, in step (1), the method for preparing Cornus officinalis powder is as follows: the dried Cornus officinalis pulp is crushed, sieved, and dried in sequence to obtain Cornus officinalis powder.

[0012] A second aspect of the present invention provides a Cornus officinalis extract obtained by the method described in the first aspect above.

[0013] Preferably, the cornus extract contains 5%–10% loganin, 8%–15% monoglycoside, 10%–18% total polysaccharides, and 3%–6% total phenolic acids.

[0014] The third aspect of this invention provides the application of the Cornus officinalis extract described in the second aspect above in the preparation of highly active compressed candies.

[0015] The fourth aspect of this invention provides a highly active compressed candy containing Cornus officinalis extract, made from the following raw materials in parts by weight: 30-50 parts Cornus officinalis extract, 20-30 parts maltitol, 15-25 parts erythritol, 8-12 parts microcrystalline cellulose, 3-5 parts crospovidone, 0.5-1.5 parts magnesium stearate, 0.3-0.8 parts citric acid, and 0.1-0.3 parts natural orange flavoring; wherein the Cornus officinalis extract is the Cornus officinalis extract described in the second aspect above.

[0016] Preferably, the pH value of the highly active compressed candy containing Cornus officinalis extract is 6.5 to 7.5.

[0017] The fifth aspect of this invention provides a method for preparing highly active compressed candies containing Cornus officinalis extract as described in the fourth aspect above, comprising the following steps: A. Mix Cornus officinalis extract with maltitol, erythritol, microcrystalline cellulose, crospovidone, and citric acid evenly, then add a binder to make a soft material. The soft material is then sieved, dried, and granulated to obtain granules. B. Add magnesium stearate and natural orange flavoring to the granules, mix well, and then compress into tablets to obtain highly active compressed candies containing Cornus officinalis extract.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The present invention adopts a compound enzymatic hydrolysis-ultrasound and microwave synergistic extraction process. First, the cell wall structure of Cornus officinalis is destroyed by the compound enzymatic hydrolysis of cellulase and pectinase to promote the release of total phenolic acid and other components. Then, ultrasound-microwave synergistic extraction is adopted. The cavitation effect of ultrasound and the thermal effect of microwave are used to accelerate the dissolution of iridoid glycosides and polysaccharides. The yield of each active ingredient is improved compared with the traditional reflux extraction.

[0019] (2) The present invention adopts an intermittent extraction mode to avoid excessive local temperature caused by continuous ultrasonic-microwave action, reduce the degradation of active ingredients, and improve product stability; the extraction solvent is ethanol, which can be recycled, with short extraction time and low energy consumption, making it suitable for industrial production.

[0020] (3) The present invention uses Cornus officinalis extract as the core functional ingredient, and the excipients are maltitol and erythritol compounded, which are low in calories and do not affect blood sugar, making them suitable for middle-aged and elderly people; microcrystalline cellulose and cross-linked polyvinylpyrrolidone are combined to ensure tablet hardness and disintegration performance; citric acid and natural orange flavoring improve palatability and mask the sour and astringent taste of Cornus officinalis itself.

[0021] (4) The total amount of iridoid glycosides of the present invention is ≥5%, the total amount of polysaccharides is ≥8%, and the total amount of phenolic acids is ≥3%, which is significantly higher than that of existing similar products and has high activity; the pH value is controlled in the neutral range of 6.5 to 7.5, which has no gastrointestinal irritation, high safety, and meets the food requirements of food and medicine homology; the sour taste is masked by the compounding of excipients, the taste is moderately sweet and sour, and the palatability is good, making it suitable for long-term consumption. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below through embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0023] (a) Cornus officinalis extract 1. Preparation of Cornus officinalis extract samples Example 1: A method for extracting Cornus officinalis extract, the specific steps of which are as follows: (1) Raw material pretreatment: Select dried fruit pulp of Cornus officinalis, crush it and pass it through a 70-mesh sieve, vacuum dry it at 60℃ for 2.5h, and set it aside; Compound enzymatic hydrolysis: Take 100g of Cornus officinalis powder, add deionized water at a material-to-liquid ratio of 1:18 (g / mL), adjust the pH value to 5.0, add 0.5g of compound enzyme (cellulase: pectinase = 1.5:1, enzyme activity ≥5000U / g), enzymatic hydrolysis at 52℃ for 75min, and boil for 10min to inactivate; (2) Ultrasonic-microwave synergistic extraction: Add 2200 mL of 65% ethanol solution to the enzymatic hydrolysate and place it in an ultrasonic-microwave synergistic extraction device. Set the ultrasonic power to 350 W, the microwave power to 180 W, and the extraction temperature to 65 °C. Use intermittent extraction (15 min / time, pause for 5 min, repeat twice) for a total extraction time of 50 min. Filter to obtain the extract. Concentration and drying: Concentrate the extract under reduced pressure to a relative density of 1.25 (60 °C) and spray dry (inlet air temperature 185 °C, outlet air temperature 85 °C) to obtain 32.6 g of Cornus officinalis extract powder.

[0024] Example 2: A method for extracting Cornus officinalis extract, the specific steps of which are as follows: (1) Raw material pretreatment: Select dried fruit pulp of Cornus officinalis, crush it and pass it through an 80-mesh sieve, vacuum dry it at 55℃ for 3 hours, and set it aside; Compound enzymatic hydrolysis: Take 100g of Cornus officinalis powder, add deionized water at a material-to-liquid ratio of 1:20 (g / mL), adjust the pH value to 4.8, add 0.6g of compound enzyme (cellulase: pectinase = 2:1, enzyme activity ≥5000U / g), enzymatic hydrolysis at 55℃ for 60min, and boil for 10min to inactivate; (2) Ultrasonic-microwave synergistic extraction: Add 70g of compound enzyme (cellulase: pectinase = 2:1, enzyme activity ≥5000U / g) to the enzymatic hydrolysate. 2500 mL of % ethanol solution was placed in an ultrasonic-microwave synergistic extraction device. The ultrasonic power was set to 380 W, the microwave power to 200 W, and the extraction temperature to 70 °C. Intermittent extraction was used (12 min / time, paused for 3 min, repeated 3 times) for a total extraction time of 45 min. The extract was filtered to obtain the extract. Concentration and drying: The extract was concentrated under reduced pressure to a relative density of 1.28 (60 °C) and spray-dried (inlet air temperature 190 °C, outlet air temperature 90 °C) to obtain 31.8 g of Cornus officinalis extract powder.

[0025] Example 3: A method for extracting Cornus officinalis extract, the specific steps of which are as follows: (1) Raw material pretreatment: Select dried fruit pulp of Cornus officinalis, crush it and pass it through a 60-mesh sieve, vacuum dry it at 65℃ for 2 hours, and set it aside; Compound enzymatic hydrolysis: Take 100g of Cornus officinalis powder, add deionized water at a material-to-liquid ratio of 1:15 (g / mL), adjust the pH value to 5.2, add 0.5g of compound enzyme (cellulase: pectinase = 1:1, enzyme activity ≥5000U / g), enzymatic hydrolysis at 50℃ for 90min, and boil for 10min to inactivate; (2) Ultrasonic-microwave synergistic extraction: Add 2000 mL of 60% ethanol solution to the enzymatic hydrolysate and place it in an ultrasonic-microwave synergistic extraction device. Set the ultrasonic power to 320 W, the microwave power to 160 W, and the extraction temperature to 60 °C. Use intermittent extraction (total extraction time 40 min) and filter to obtain the extract. Concentration and drying: Concentrate the extract under reduced pressure to a relative density of 1.22 (60 °C) and spray dry (inlet air temperature 180 °C, outlet air temperature 80 °C) to obtain 33.5 g of Cornus officinalis extract powder.

[0026] 2. Component analysis and yield of Cornus officinalis extract 2.1 Method for determining the content of loganin Accurately weigh 0.5–1.0 g and place it in a 50 mL centrifuge tube. Add 25–50 mL of methanol and extract ultrasonically for 30 min (200 W, 30 °C). Shake well and centrifuge at 8000 rpm for 10 min. Filter the supernatant through a 0.45 μm organic phase filter membrane for analysis. Mobile phase: methanol-water (30:70, v / v) or acetonitrile-0.05 mol / L potassium dihydrogen phosphate solution (25:75, v / v, pH adjusted to 4.0). Elute isocratically (gradient elution can be used for complex samples). Flow rate: 1.0 mL / min; column temperature: 30 °C; detection wavelength: 235 nm; injection volume: 10–20 μL.

[0027] Preparation of standard curve: Accurately weigh an appropriate amount of strychnine reference standard, dissolve and dilute it with methanol to prepare a series of standard solutions of 0.02, 0.05, 0.1, 0.2, and 0.5 mg / mL. Inject the solutions under the chromatographic conditions described above, plot the standard curve as peak area (y) versus concentration (x), and calculate the regression equation (R²). 2 ≥0.999). Sample determination: Inject the sample filtrate, record the peak area, and substitute it into the regression equation to calculate the strychnine content.

[0028] 2.1 Moroniside content detection method Detection wavelength: 238 nm (slightly higher than strychnine; the DAD detector can simultaneously acquire spectra of 235–240 nm for optimized separation); Mobile phase: acetonitrile-water gradient elution (e.g., 0–10 min, acetonitrile 15% → 25%; 10–20 min, 25% → 30%), to achieve baseline separation of the two.

[0029] Pretreatment: Same as strychnine (methanol / ethanol ultrasonic extraction); Chromatographic column: C18 column (4.6 mm × 250 mm, 5 μm); mobile phase: acetonitrile-0.1% phosphoric acid solution (20:80, v / v); flow rate: 1.0 mL / min; column temperature: 30℃; detection wavelength: 238 nm.

[0030] Monoglycosides are slightly more polar than strychnine, and the proportion of aqueous phase in the mobile phase can be appropriately increased (e.g., 75%–85%) to shorten the retention time; 2.3 Method for determining total polysaccharide content Phenol-sulfuric acid colorimetric method Reagents: Glucose reference standard (purity ≥99%), phenol (analytical grade, redistilled to prepare 5% phenol solution: take 5 g phenol, add water to 100 mL, and store in the dark under cold conditions), concentrated sulfuric acid (analytical grade), anhydrous ethanol (analytical grade, used for protein removal).

[0031] Accurately weigh 1.0 g of sample, add 80 mL of distilled water, extract in a boiling water bath for 2 h, cool, filter, and transfer the filtrate to a 100 mL volumetric flask, then dilute to volume with water. Protein removal: Take 20 mL of the above extract, add an equal volume of anhydrous ethanol (final concentration 50%), shake well, let stand overnight, centrifuge at 8000 r / min for 15 min, and discard the supernatant (to remove alcohol-soluble impurities such as proteins and monosaccharides). Reconstitution: Dissolve the precipitate in 20 mL of distilled water, transfer to a 50 mL volumetric flask, dilute to volume with water, filter through a 0.45 μm aqueous phase filter membrane, and proceed with analysis (if turbidity persists, centrifuge again).

[0032] Preparation of standard curve: Accurately weigh 10 mg of glucose reference standard, dissolve it in water and dilute to 100 mL to obtain a 0.1 mg / mL standard stock solution; Take 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mL of the stock solution respectively and place them into 10 mL stoppered test tubes, then add water to make up to 2.0 mL; Add 1.0 mL of 5% phenol solution, shake well, and then quickly add 5.0 mL of concentrated sulfuric acid (add slowly along the tube wall to avoid local overheating), shake well immediately; heat in a boiling water bath for 15 min, remove and cool to room temperature, using a blank tube (2.0 mL water + 1.0 mL phenol + 5.0 mL concentrated sulfuric acid) as a reference, measure the absorbance at 490 nm; plot a standard curve of absorbance (y) against glucose concentration (x, μg / mL), and obtain the regression equation (R²). 2 ≥0.999).

[0033] Take 0.5–1.0 mL of the test solution (adjust according to the polysaccharide content to ensure the absorbance is within the standard curve range), place it in a 10 mL stoppered test tube, and add water to bring the volume to 2.0 mL; the subsequent steps are the same as for the standard curve (add phenol and concentrated sulfuric acid, boil in a water bath, cool, and measure the absorbance). Calculation: Obtain the "glucose equivalent" concentration in the sample based on the regression equation, and then convert it to the total polysaccharide content (total polysaccharide % = (measured concentration × dilution factor × conversion factor) / sample amount × 100%, the conversion factor is adjusted according to the purity of the sample polysaccharide, unless otherwise specified, it is directly calculated as glucose).

[0034] 2.4 Determination method for total phenolic acids Folin-Ciocalteu method (gallic acid reference standard (purity ≥98%), Folin-Ciocalteu reagent (can be purchased directly or prepared in-house), sodium carbonate (analytical grade, to prepare 10% sodium carbonate solution: 10 g Na2CO3 added to 100 mL of water), ethanol (analytical grade, 50% ethanol used for extraction).

[0035] Accurately weigh 0.5 g of sample and place it in a 50 mL centrifuge tube. Add 25 mL of 50% ethanol and sonicate for 40 min (power 200 W, temperature 30℃). Shake well and centrifuge at 8000 r / min for 10 min. Take the supernatant and dilute it with 50% ethanol to a suitable concentration (so that the absorbance is within the range of the standard curve) for testing.

[0036] Preparation of standard curve: Accurately weigh 10 mg of gallic acid reference standard, dissolve it in 50% ethanol and dilute to 100 mL to obtain a 0.1 mg / mL standard stock solution; take 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 mL of the stock solution respectively and place them in 10 mL stoppered test tubes, add 50% ethanol to make up to 2.0 mL; add 0.5 mL of Folin reagent, shake well and let stand for 3 min, then add 2.5 mL of 10% sodium carbonate solution and shake well; let stand at room temperature in the dark for 60 min, and use a blank tube (2.0 mL 50% ethanol + 0.5 mL Folin reagent + 2.5 mL sodium carbonate solution) as a reference, measure the absorbance at 760 nm; plot the standard curve with absorbance (y) against gallic acid concentration (x, μg / mL) to obtain the regression equation (R²≥0.999).

[0037] Take 1.0–2.0 mL of the test solution and place it in a 10 mL stoppered test tube, then add 50% ethanol to bring the volume to 2.0 mL. The subsequent steps are the same as for the standard curve (add Folin reagent and sodium carbonate, let stand in the dark for 60 min, and measure the absorbance). Calculation: Based on the regression equation, the concentration of "gallic acid equivalent" in the sample is obtained, and the total phenolic acid content is converted (total phenolic acid % = (measured concentration × dilution factor) / sample amount × 100%).

[0038] 2.4 Formula for calculating the yield of Cornus officinalis extract: Cornus officinalis extract yield = m1 / m2 × 100% In the formula: m1 is the oven-dry mass (g) of Cornus officinalis extract after spray drying; m2 is the oven-dry mass (g) of Cornus officinalis powder.

[0039] The results of component detection and yield calculation of Cornus officinalis extract in Examples 1 to 3 of this invention are shown in Table 1.

[0040] Table 1. Composition and yield of Cornus officinalis extract in Examples 1-3 As shown in Table 1, Example 2 increased the mass ratio of cellulase in the enzyme system, increased the enzymatic hydrolysis temperature, and improved the extraction process parameters. Through the synergistic optimization of these process parameters, the dissolution and release efficiency of the target active ingredients (loganin, monoglycosides, and total polysaccharides) in Cornus officinalis was significantly improved. The test results showed that the content of loganin, monoglycosides, and total polysaccharides in the product obtained in this example was increased by 1.5% to 6.3% compared with Example 1. Example 3 reduced the enzymatic hydrolysis temperature, shortened the enzymatic hydrolysis time, and improved the extraction process parameters. The optimized process conditions of Example 1 were not used, resulting in a slight decrease in the extraction efficiency of the target active ingredients in Cornus officinalis. The test results showed that the content of loganin, monoglycosides, and total polysaccharides in the product obtained in this example was slightly reduced compared with Example 1.

[0041] (II) High-activity compressed candy 1. Preparation of high-activity compressed candy samples Example 4: A method for preparing a highly active compressed candy containing Cornus officinalis extract, the specific steps of which are as follows: A. Mixing and granulation: Take 40g of Cornus officinalis extract from Example 1, add 25g maltitol, 20g erythritol, 10g microcrystalline cellulose, 4g crospovidone, and 0.5g citric acid, mix evenly, use 70% ethanol as a binder to make a soft mass, granulate through a 20-mesh sieve, dry at 60℃ for 2h, and then granulate through an 18-mesh sieve. B. Tableting and Packaging: Add 1g magnesium stearate and 0.2g natural orange flavoring, mix evenly, compress into tablets, with a tablet weight of 0.5g / tablet and a hardness of 4.2kg. Vacuum packaging yields highly active compressed candy containing Cornus officinalis extract.

[0042] Example 5: A method for preparing a highly active compressed candy containing Cornus officinalis extract, the specific steps of which are as follows: A. Mixing and granulation: Take 40g of Cornus officinalis extract from Example 2, mannitol, 23g xylitol, 12g microcrystalline cellulose, 3g crospovidone, and 0.6g citric acid, mix them evenly, use 80% ethanol as a binder to make a soft mass, granulate it through a 20-mesh sieve, dry it at 60℃ for 2.5h, and then granulate it through an 18-mesh sieve. B. Tableting and Packaging: Add 0.8g of sodium stearate fumarate and 0.3g of natural lemon flavoring, mix well, compress into tablets, with a tablet weight of 0.5g / tablet and a hardness of 4.5kg. Vacuum packaging yields highly active compressed candies containing Cornus officinalis extract. Example 6: A method for preparing a highly active compressed candy containing Cornus officinalis extract, the specific steps of which are as follows: A. Mixing and granulation: Take 40g of Cornus officinalis extract from Example 3, add 30g of sorbitol, 18g of erythritol, 8g of microcrystalline cellulose, 5g of crospovidone, and 0.4g of citric acid, mix evenly, use 60% ethanol as a binder to make a soft mass, granulate through a 20-mesh sieve, dry at 55℃ for 3h, and then granulate through an 18-mesh sieve. B. Tableting and Packaging: Add 1.2g magnesium stearate and 0.1g natural peppermint flavoring, mix well and compress into tablets. The tablet weight is 0.5g / tablet, the hardness is 4.0kg, and vacuum packaging yields high-activity compressed candy containing Cornus officinalis extract.

[0043] 2. Performance Testing of High-Activity Compressed Candy Tablets 2.1 Tablet hardness test Test Method: According to the 2020 edition of the Chinese Pharmacopoeia, Part IV, General Chapter 0921, Tablet Hardness Determination, a YD-30 tablet hardness tester was used. Ten tablets were randomly selected from the prepared compressed candy as test samples. The tablets were placed sequentially on the measuring platform of the hardness tester, and the clamps were adjusted to fix the tablets and prevent them from shifting under force. The instrument was started so that the upper indenter applied pressure downwards at a uniform speed until the tablet broke. The pressure value at the time of each tablet's breakage was recorded, and the average value of the 10 tablets was calculated as the hardness value of the sample in this example.

[0044] 2.2 Collapse Time Limit Test Test Method: The disintegration time test was performed according to the General Chapter 0921 of the 2020 edition of the Chinese Pharmacopoeia, Part IV, using a ZB-1C intelligent disintegration time meter. An appropriate amount of purified water at 37℃±1℃ was injected into the beaker of the disintegrator. The water level was adjusted so that the sieve was 25mm±2mm from the bottom of the beaker when the lifting basket descended. Six tablets were randomly selected from the prepared compressed candy as test samples and placed in the six baskets of the disintegrator. The instrument was started, and the time from the start of the test to complete disintegration (all disintegrated particles passed through the sieve without any obvious lumps) was recorded. The average value of the six tablets was taken as the disintegration time of the sample in this example.

[0045] The performance test results of the highly active compressed candies of Examples 4 to 6 of the present invention are shown in Table 2.

[0046] Table 2 Performance test results of high-activity compressed candies from Examples 4 to 6 As shown in Table 2, the key parameters of the high-activity compressed candy tablets (hardness and disintegration time) all meet the requirements of the General Rules for Tablets in the Chinese Pharmacopoeia (hardness 3-5 kg, disintegration time ≤ 60 s), and the adjustment of excipients did not affect the stability of the formulation.

[0047] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention. Those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention based on the concept of the present invention, without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for extracting Cornus officinalis extract, characterized in that, Includes the following steps: (1) Mix Cornus officinalis powder with water at a ratio of 1g:(15-20)mL to obtain a mixture. Adjust the pH of the mixture to 4.5-5.

5. Then add 0.3%-0.8% of the mass of Cornus officinalis powder with a compound enzyme. Enzymatically hydrolyze the mixture at 50-55℃ for 60-90 minutes to obtain an enzyme hydrolysate. Then boil the enzyme hydrolysate to inactivate it. (2) Add ethanol solution to the inactivated enzyme hydrolysate, and then place it in an ultrasonic-microwave synergistic extraction device for extraction. After extraction, filter and collect the Cornus officinalis extract. The Cornus officinalis extract is then concentrated and spray-dried to obtain Cornus officinalis extract.

2. The extraction method of Cornus officinalis extract as described in claim 1, characterized in that, In step (1), the complex enzyme is a mixture of cellulase and pectinase; wherein the mass ratio of cellulase to pectinase is (1-2):

1.

3. The extraction method of Cornus officinalis extract as described in claim 1, characterized in that, In step (2), the extraction conditions are: ultrasonic power of 300-400W, microwave power of 150-200W, extraction temperature of 60-70℃, and extraction time of 40-60min.

4. The extraction method of Cornus officinalis extract as described in claim 1, characterized in that, In step (2), the volume percentage concentration of the ethanol solution is 60% to 70%, and the volume ratio of the inactivated enzyme hydrolysate to the ethanol solution is 1:(20 to 25).

5. A Cornus officinalis extract obtained by the method described in any one of claims 1-4.

6. The Cornus officinalis extract according to claim 5, characterized in that, The Cornus officinalis extract contains 5%–10% loganin, 8%–15% monosodium glycoside, 10%–18% total polysaccharides, and 3%–6% total phenolic acids.

7. The use of the Cornus officinalis extract according to claim 5 or 6 in the preparation of highly active compressed candies.

8. A highly active compressed candy containing Cornus officinalis extract, characterized in that, It is made from the following raw materials in parts by weight: 30-50 parts of Cornus officinalis extract, 20-30 parts of maltitol, 15-25 parts of erythritol, 8-12 parts of microcrystalline cellulose, 3-5 parts of crospovidone, 0.5-1.5 parts of magnesium stearate, 0.3-0.8 parts of citric acid, and 0.1-0.3 parts of natural orange flavoring; wherein, the Cornus officinalis extract is the Cornus officinalis extract as described in claim 5 or 6.

9. The highly active compressed candy containing Cornus officinalis extract as described in claim 8, characterized in that, The pH value of the highly active compressed candy containing Cornus officinalis extract is 6.5–7.

5.

10. The method for preparing highly active compressed tablets containing Cornus officinalis extract as described in claim 8 or 9, characterized in that, Includes the following steps: A. Mix Cornus officinalis extract with maltitol, erythritol, microcrystalline cellulose, crospovidone, and citric acid evenly, then add a binder to make a soft material. The soft material is then sieved, dried, and granulated to obtain granules. B. Add magnesium stearate and natural orange flavoring to the granules, mix well, and then compress into tablets to obtain highly active compressed candies containing Cornus officinalis extract.

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