Application of artificial synthetic carrier RNA for improving sensitivity of exogenous nucleic acid detection in nucleic acid detection

Abstract

The application provides application of an artificial synthetic carrier RNA in nucleic acid detection, which is used for improving the sensitivity of exogenous nucleic acid detection, the nucleic acid sequence of the carrier RNA has high homology or cross-complementarity with the rRNA sequence of the endogenous host in the sample to be detected, and the rRNA sequence is the whole or partial sequence of 18S rRNA and / or 28S rRNA or an RNA sequence having at least 90% sequence homology with the whole or partial sequence of 18S rRNA and / or 28S rRNA. The new high-sensitivity exogenous nucleic acid detection technology provided by the application solves the key problems in the prior art, such as poor sample universality, large sequencing background interference in the existing carrier RNA use scheme and low nucleic acid detection sensitivity, significantly improves the effective proportion of trace RNA virus nucleic acid in the final sequencing library, and thus greatly improves the nucleic acid detection sensitivity of viruses and the like.

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