Gene PbMYB20 for regulating formation of stone cells of pear fruits

By cloning the PbMYB20 gene in pear fruit and regulating its expression level, the problem of the intrinsic regulatory mechanism of pear fruit stone cell formation was solved, the lignin content was regulated, the fruit quality was improved, and a gene resource for fruit quality breeding was provided.

CN122012608AActive Publication Date: 2026-05-12SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY +2
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Patent Information

Application Number
CN202610500285.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-16
Publication Date
2026-05-12
Estimated Expiration
2046-04-16

AI Technical Summary

Technical Problem

Existing technologies have failed to fully elucidate the intrinsic regulatory mechanism of pear fruit stone cell formation, resulting in excessively high stone cell content that affects fruit quality, reduces taste, and diminishes market value.

Method used

The key gene PbMYB20, which regulates stone cell formation in pear fruit, was cloned and isolated. By constructing overexpression and silencing vectors, the expression level of PbMYB20 was regulated in Arabidopsis thaliana and pear fruit, thereby increasing or decreasing lignin content and thus regulating stone cell formation.

Benefits of technology

Significantly increasing or decreasing the lignin content in pear fruits and Arabidopsis thaliana improves fruit quality, promotes fruit quality enhancement and efficiency, and provides new genetic resources for fruit quality breeding.

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Abstract

The invention discloses a gene PbMYB20 for regulating and controlling synthesis of pear fruit lignin, the CDS sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a coded protein is as shown in SEQ ID NO.2. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the content of stone cells and lignin is remarkably increased compared with that of injection of a no-load control part; when a silent vector constructed by the gene is injected into a Dangshan pear fruit, the stone cell content and the lignin content are obviously reduced compared with those of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, the secondary cell wall of conduit cells of the obtained PbMYB20 transgenic arabidopsis thaliana is obviously thickened, and the accumulation of lignin in stems is obviously increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.
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