A library construction method, a library construction device, and a library construction kit
By adding markers to genomic DNA and cell-free DNA during library construction, the problem of separate library construction in existing technologies is solved, enabling merged library construction, reducing costs and improving efficiency.
CN122105641APending Publication Date: 2026-05-29BEIJING BOE TECH DEV CO LTD +1
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Patent Information
- Application Number
- CN202411735122.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-28
- Publication Date
- 2026-05-29
AI Technical Summary
Technical Problem
In existing technologies, genomic DNA and cell-free DNA, due to their similar fragment sizes but different molecular characteristics, require separate library construction, which increases workload and sequencing costs.
Method used
During library construction, markers are added to genomic DNA and cell-free DNA samples. Samples are distinguished by insert sequences, adapter sequences, or short double-stranded nucleotide sequences, and then merged to build the library. The markers are used to distinguish samples during sequencing.
Benefits of technology
This method enables the combined library construction of two types of samples, reducing library construction time and reagent consumption, lowering costs, and improving library construction efficiency.
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Figure CN122105641A_ABST
Abstract
The application discloses a library construction method, a library construction device and a library construction kit. The library construction method of one embodiment comprises the following steps: extracting a nucleic acid sample from a genetic information carrier, wherein the nucleic acid sample comprises a first sample and a second sample; adding a label to at least one of the first sample and the second sample, and merging the first sample and the second sample to establish a library; performing sequencing on the established library, and distinguishing the first sample and the second sample according to the label. The embodiment provided by the application adds a label to at least one of the first sample and the second sample after extracting the first sample and the second sample in the process of establishing a library, and distinguishes the first sample and the second sample by using the label in sequencing, so that the merging of two types of samples is realized, the library construction time is reduced, the library construction efficiency is improved, the amount of reagent consumables is reduced, and the cost is reduced.
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