Reverse transcriptase mutant f9k and prime editors containing same and applications

By performing site-directed mutagenesis on reverse transcriptase, a reverse transcriptase mutant F9K was developed, which solved the problem of insufficient catalytic activity of existing reverse transcriptases, enabling efficient gene editing operations and improving the editing efficiency and accuracy of the guide editor.

CN122303187APending Publication Date: 2026-06-30INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Filing Date
2026-04-28
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing reverse transcriptases have limited catalytic activity, making it difficult to meet the needs of efficient gene editing and limiting the editing efficiency and accuracy of guide editors.

Method used

A reverse transcriptase mutant, F9K, was developed by site-directedly mutagenesizing the wild-type reverse transcriptase from Sphaerotilaceae, changing the phenylalanine at position 9 to lysine, thereby enhancing its gene editing activity. A guide editor was constructed by combining the Cas9 protein and guide RNA.

Benefits of technology

The reverse transcriptase mutant F9K significantly enhances gene editing activity, enabling efficient gene editing operations, including insertion, deletion, substitution, and point mutation, overcoming the limitations of traditional genome editing tools and reducing off-target effects.

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Abstract

The present application relates to the technical field of gene editing, in particular to a reverse transcriptase mutant F9K, a prime editor containing the same and application. The present application provides a novel reverse transcriptase mutant F9K, which is verified to have high gene editing activity through a functional recovery experiment of chloramphenicol resistance gene, and can be applied to the field of gene editing. The present application provides a new molecular tool for gene editing, and has important value for the field of gene editing.
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