A neuroendocrine metabolite detection kit and a detection method thereof

By employing high-performance liquid chromatography-tandem mass spectrometry and stable isotope-labeled internal standards, the problems of detection accuracy and multi-indicator joint detection in the screening of neuroendocrine tumors and the auxiliary diagnosis of diseases such as depression and Parkinson's disease have been solved. This has achieved highly specific, accurate, and simplified urine testing, providing a reliable diagnostic basis.

CN122330307APending Publication Date: 2026-07-03HEBEI QIANYE BIOTECHNOLOGY CO LTD
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEBEI QIANYE BIOTECHNOLOGY CO LTD
Filing Date
2026-03-19
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing technologies lack accuracy in screening for neuroendocrine tumors and in the auxiliary diagnosis of diseases such as depression and Parkinson's disease. Traditional detection methods are prone to false positives and cross-reactions, suffer from severe interference from complex sample matrices, have low sensitivity, cannot achieve joint detection of multiple indicators, and are easily affected by diet and medication. They also have low standardization and inaccurate creatinine correction, which leads to amplified errors.

Method used

High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with stable isotope-labeled internal standards was employed. Target analytes were purified using protein precipitation technology, and quantitative analysis was performed using the multiple recognition mechanism of mass spectrometry. Creatinine was introduced to correct for changes in urine concentration, enabling simultaneous detection of multiple indicators.

Benefits of technology

It improves the specificity and accuracy of detection, simplifies the sample collection process, reduces errors, provides reliable diagnostic evidence, expands the application range of random urine, and can accurately assess the levels of related substances in neuroendocrine tumors and mental disorders.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122330307A_ABST
    Figure CN122330307A_ABST
Patent Text Reader

Abstract

The present application relates to a kind of neuroendocrine metabolite detection kit and its detection method, it is related to biological detection technical field.The kit includes including calibration, high-value quality control, low-value quality control, internal standard solution and precipitant.The detection method related to the present application includes the following steps: S1, sample pretreatment: S2, machine detection;S3, calibration curve drawing;S4, quality control data analysis;S5, sample data analysis.The present application detects accurately, error is small, simple operation avoids the cross reaction caused by structural analogue, ensures the authenticity of detection result.In the accuracy, mass spectrometry uses stable isotope-labeled internal standard to carry out absolute quantification, can accurately compensate the loss and matrix effect in sample pretreatment process, to provide highly accurate and repeatable quantitative result, provides important basis for neuroendocrine tumor screening and auxiliary diagnosis of depression, Parkinson's disease and the like.
Need to check novelty before this filing date? Find Prior Art