A magnetic microsphere separation and analysis device for target fishing of active ingredients of dioscorea opposita

Abstract

The present application relates to active ingredient separation detection technical field, and disclose a kind of for Chinese yam active ingredient target fishing magnetic microsphere separation analysis device, including the sample pretreatment module, magnetic microsphere incubation combination module, magnetic field separation module, elution purification module and target analysis detection module in turn by sealed conveying pipeline communication;The magnetic field separation module is used to provide adjustable magnetic field gradient, realize the separation enrichment of the magnetic microsphere combined with active ingredient-target complex and the separation of uncombined impurities.This magnetic microsphere separation analysis device for Chinese yam active ingredient target fishing realizes the lossless, pollution prevention transmission of material between each module, completes the automatic integrated operation from Chinese yam extract processing to target qualitative and quantitative detection, greatly improves the overall work efficiency of Chinese yam active ingredient target fishing, effectively avoids the pollution risk introduced by manual operation, ensures the reliability of experimental results.

Description

Technical Field

[0001] This invention belongs to the field of active ingredient separation and detection technology, specifically relating to a magnetic microsphere separation and analysis device for target fishing of active ingredients in yam. Background Technology

[0002] As a traditional food and medicine ingredient, yam contains active ingredients such as polysaccharides, saponins, and polyphenols, which have important pharmacological activities and physiological functions. Precisely targeting and analyzing the active ingredients is the core link in elucidating the pharmacological mechanism of yam and developing related functional products. Magnetic microsphere separation technology has become the mainstream technology for targeting active ingredients in natural products due to its advantages of high specificity and high separation efficiency.

[0003] Currently, when using magnetic microsphere technology to target and extract active ingredients from yam, the process is mostly done manually combined with dispersed equipment. The entire process requires step-by-step operation using sample processing equipment, constant temperature incubation equipment, magnetic field separation device, purification equipment, and detection instruments. Each step requires manual material transfer, operation switching, and parameter adjustment. This not only makes the overall operation process cumbersome and significantly reduces the efficiency of target extraction, but also makes it easy to introduce external contamination during manual material transfer, resulting in the loss of target materials and affecting the accuracy and reliability of experimental results.

[0004] In the core magnetic field separation stage, existing separation devices have significant technical defects: First, the magnetic field design is simplistic, often using a fixed intensity magnetic field for separation, which cannot achieve gradient magnetic separation. This makes it difficult to simultaneously remove strong magnetic impurities and enrich the target magnetic microsphere complex, easily leading to strong magnetic impurities interfering with subsequent separation and detection, or incomplete separation of the target substance from non-magnetic impurities such as starch and dietary fiber, resulting in low target substance recovery rate and poor purity. Second, the contact area and contact time between the magnetic field and the material are limited, and the magnetic microsphere complex is easily lost with the liquid phase. Furthermore, there is a lack of a precise trapping structure for weak target substances remaining during the flow process, further reducing separation efficiency. Third, the design of the impurity and target substance export structures is unreasonable, easily leading to material mixing and making it impossible to achieve precise classification and export of the two, affecting the purity of the raw materials in subsequent elution and purification stages. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a magnetic microsphere separation and analysis device for target fishing of active ingredients in yam, thereby solving the problems of poor magnetic field separation effect and low recovery rate and purity of target substances in the prior art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a magnetic microsphere separation and analysis device for target extraction of active ingredients from yam, comprising a sample pretreatment module, a magnetic microsphere incubation and bonding module, a magnetic field separation module, an elution and purification module, and a target analysis and detection module connected sequentially through a sealed delivery pipeline;

[0007] The magnetic field separation module is used to provide a gradient-adjustable magnetic field to achieve the separation and enrichment of magnetic microspheres bound with active ingredient-target complexes and the separation of unbound impurities. The magnetic field separation module includes a cylindrical separation container, and the interior of the separation container is provided with an impurity separation zone and a magnetic bead separation and enrichment zone from top to bottom. An impurity processing component is provided in the impurity separation zone, including a first magnetic field generator. The magnetic field of the first magnetic field generator acts on the impurity separation zone to separate a small amount of strong magnetic impurities contained in the magnetic microspheres of the active ingredient-target complex by weak magnetic force. The magnetic bead separation and enrichment area is equipped with an enrichment component and an export component; The enrichment component includes a second magnetic field generator. The magnetic field of the second magnetic field generator acts on the magnetic bead separation and enrichment zone to specifically adsorb the target magnetic microsphere complex, so that the magnetic microsphere complex is attached to the sidewall of the magnetic bead separation and enrichment zone. The export component is used to classify and export the separated magnetic microsphere complex and unbound impurities such as starch and dietary fiber.

[0008] Preferably, the sample pretreatment module is used to filter, remove impurities from, and adjust the concentration of the yam extract; The sample pretreatment module includes a storage tank for storing samples, a filtration assembly for sample pretreatment, a concentration adjustment chamber, and a delivery pump.

[0009] Preferably, the magnetic microsphere incubation and binding module is used to load magnetic microspheres that are coupled with target probes, and to achieve constant temperature incubation and specific binding of magnetic microspheres with active ingredients in yam extract; The magnetic microsphere incubation module includes a constant-temperature incubation chamber for storing the pretreatment liquid, a loading box for injecting magnetic microspheres into the constant-temperature incubation chamber, and a stirring assembly and a pH adjustment assembly for processing the mixture.

[0010] Preferably, the elution and purification module is used to perform gradient elution on the enriched magnetic microsphere complex to obtain purified yam active ingredient-target complex. The elution and purification module includes an eluent storage component, a gradient elution chamber, and a purification filtration component.

[0011] Preferably, the target analysis and detection module is used to perform qualitative identification and quantitative detection of targets on the eluted complex; The target analysis and detection module includes an injection port, a high-performance liquid chromatography (HPLC) detection unit, a mass spectrometry (MS) detection unit, and a data recording component. The injection port is connected to the outlet of the elution and purification module. The HPLC detection unit and the MS detection unit are connected in series. The data recording component includes a detector and a data storage module. The detector is used to collect chromatograms and mass spectra of the target detection.

[0012] Preferably, the enrichment component further includes several arc-shaped ribs fixed to the inner wall of the magnetic bead separation enrichment area, and the several arc-shaped ribs are arranged in a circular array.

[0013] The separation container is externally fixedly connected to a motor drive group for the annular displacement of the second magnetic field generator. The magnetic field of the second magnetic field generator covers the entire height of the arc-shaped ribs, and the liquid level in the magnetic bead separation enrichment zone is lower than the middle of the arc-shaped ribs. When the second magnetic field generator moves in annular motion, it drives the magnetic bead complex attached to the side wall of the magnetic bead separation enrichment zone to move upward along the support surface of several arc-shaped ribs, thereby increasing the adhesion surface of the magnetic bead complex.

[0014] Preferably, the export component includes an impurity discharge tube and a complex discharge tube connected to the interior of the magnetic bead separation and enrichment zone; The impurity discharge pipe is fixedly connected to the bottom of the separation container; The top end of the complex discharge pipe is fixedly connected to a funnel-shaped support plate, which is located below the magnetic bead separation and enrichment zone. An annular gap is reserved between the funnel-shaped support plate and the separation container for the downward discharge of impurities. The magnetic field of the second magnetic field generator acts on the annular gap to precisely adsorb the weak magnetic bead complex residue in the liquid flowing down through the annular gap.

[0015] Preferably, the bucket-shaped support plate includes a rubber part T1 and a rigid part T2, wherein the rigid part T2 is fixed to the inside of the separation container by a bracket; An expandable sealing airbag is embedded in the outer edge of the rigid part T2, which is used to seal the annular gap by expanding the sealing airbag, so that the magnetic bead compound on the bucket-shaped support plate can be discharged separately through the compound discharge pipe. The bottom end of the compound discharge pipe extends to the bottom of the separation container, and a sealing sleeve is provided at the part through which it penetrates the separation container. An adjusting cylinder for adjusting the compound discharge pipe up and down is fixedly connected to the separation container. When the complex discharge tube moves upward, the top of the complex discharge tube is sealed with the sealing plug fixed in the magnetic bead separation and enrichment area, and the rubber part T1 of the bucket-shaped support plate is in an upward convex state, so that the non-magnetic impurities in the magnetic bead separation and enrichment area are discharged downward through the annular gap. When the composite discharge tube moves downward, the sealing plug loses its blockage of the composite discharge tube, and the rubber part T1 of the bucket-shaped support plate is concave. The sealing airbag seals the annular gap and demagnetizes the magnetic bead separation enrichment area, allowing the separated magnetic microsphere composite to be discharged through the composite discharge tube.

[0016] Preferably, the impurity treatment component further includes a feeding pipe connected to the top of the container via a support, and a dispersing hopper Q is fixedly connected to the bottom end of the feeding pipe. A gap is reserved between the outer edge of the dispersing hopper Q and the inner wall of the impurity separation zone, which is used to disperse the compound inside the feeding pipe to the inner surface of the impurity separation zone, so that the compound adheres to the inner wall of the impurity separation zone and flows down. Then, the magnetic field of the first magnetic field generator causes the strong magnetic impurities in the compound to remain on the inner wall of the impurity separation zone, thus forming separation. The magnetic field strength of the first magnetic field generator is 0.1~0.2T, and it is used to adsorb strong magnetic impurities individually.

[0017] Preferably, the inner wall of the impurity separation zone is provided with an annular receiving groove, and the opening of the receiving groove is located above the gap opening; The first magnetic field generator is axially slidably connected to the inner surface of the separation container, and its height is adjusted by an electric telescopic rod fixed to the separation container. The upward displacement of the first magnetic field generator drives the strong magnetic impurities attached to the impurity separation zone to move upward. A magnetic isolation sleeve is installed in the lining of the impurity separation zone to isolate the magnetic field of the first magnetic field generator, so that when the strong magnetic impurities move to the opening of the receiving tank, they lose their magnetic attraction and automatically enter the receiving tank.

[0018] Compared with the prior art, the present invention provides a magnetic microsphere separation and analysis device for target extraction of active ingredients from yam, which has the following beneficial effects: This invention comprises five modules connected sequentially via sealed delivery pipelines: sample pretreatment, magnetic microsphere incubation and binding, magnetic field separation, elution and purification, and target analysis and detection. Following the core process of "sample pretreatment - specific binding - magnetic field separation - purification - detection," it achieves lossless and contamination-free material transfer between modules, completing an automated integrated operation from yam extract processing to qualitative and quantitative target detection. This significantly improves the overall efficiency of yam active ingredient target extraction while effectively avoiding the contamination risks introduced by manual operation, ensuring the reliability of experimental results.

[0019] The magnetic field separation module of this invention serves as the core functional module. It employs a gradient-adjustable magnetic field design, using a weak magnetic field of 0.1~0.2T in the impurity separation zone to achieve targeted separation of strong magnetic impurities. Then, a strong magnetic field in the magnetic bead separation and enrichment zone completes the directional adsorption of the target magnetic microsphere complex, achieving a stepwise impurity removal and directional enrichment staged separation effect. Combined with thin-film flow distribution technology, dynamic magnetic field scanning technology, and magnetic trapping technology, the contact area between the magnetic field and the material is increased, and the contact time is extended. Simultaneously, it precisely adsorbs any weak target substances remaining during the flow process, effectively preventing target substance loss. Compared to traditional separation methods, this significantly improves the separation efficiency and recovery rate of the active ingredient-target complex, solving the problems of low separation efficiency and high loss rate of target substances and impurities in traditional technologies.

[0020] The impurity treatment component of this invention uses an electric telescopic rod to drive the first magnetic field generator to move up and down. Combined with the magnetic shielding effect of the magnetic isolation sleeve, it realizes the automated adsorption, transportation, and collection of strong magnetic impurities, preventing them from interfering with subsequent separation and detection. The arc-shaped ribs of the enrichment component increase the effective magnetic adsorption area, and together with the annular dynamic scanning of the second magnetic field generator, further improve the adsorption effect of the target substance. The export component adopts a dual-pipeline classification export design. By adjusting the cylinder, the composite discharge pipe is moved up and down, and the shape change of the bucket-shaped support plate and the expansion and sealing of the sealing airbag are controlled in conjunction to achieve precise separation and export of non-magnetic impurities and target composites, avoiding material mixing. Attached Figure Description

[0021] Figure 1 This is a system block diagram of the magnetic microsphere separation and analysis device for target fishing of active ingredients in yam according to the present invention; Figure 2 This is a schematic diagram of the magnetic field separation module of the present invention; Figure 3 This is a cross-sectional view of the magnetic field separation module of the present invention; Figure 4 This is a cross-sectional view of the structure of the impurity treatment component of the present invention; Figure 5 This is a structural cross-sectional view of the component derived from the present invention; Figure 6 For the present invention Figure 5 A magnified view of point A in the image.

[0022] In the diagram: 100, Sample pretreatment module; 200. Magnetic microsphere incubation module; 300. Magnetic field separation module; 310. Separation container; 320. Impurity handling assembly; 321. First magnetic field generator; 322. Injection pipe; 323. Receiving tank; 324. Electric telescopic rod; 325. Magnetic isolation sleeve; 330. Enrichment component; 331. Second magnetic field generator; 332. Arc-shaped ribs; 333. Motor drive assembly; 340. Outlet component; 341. Impurity discharge pipe; 342. Complex discharge pipe; 343. Bucket-shaped support plate; 344. Sealing airbag; 345. Adjusting cylinder; 346. Sealing plug; 347. Pressure cylinder; 348. Piston plate; 400. Elution and purification module; 500. Target analysis and detection module. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0024] Example 1: See attached document Figures 1 to 6 A magnetic microsphere separation and analysis device for target extraction of active ingredients from yam includes a sample pretreatment module 100, a magnetic microsphere incubation and binding module 200, a magnetic field separation module 300, an elution and purification module 400, and a target analysis and detection module 500, which are sequentially connected through a sealed delivery pipeline. The sealed delivery pipelines between the modules ensure lossless and contamination-free material transfer. The entire device follows a core target extraction process of sample pretreatment-specific binding-magnetic field separation-purification-detection, achieving automated and integrated target extraction and analysis of active ingredients from yam. The magnetic field separation module 300 is used to provide a gradient-adjustable magnetic field to achieve the separation and enrichment of magnetic microspheres bound with active ingredient-target complexes and the separation of unbound impurities. The magnetic field separation module 300 includes a cylindrical separation container 310, and the interior of the separation container 310 is provided with an impurity separation zone and a magnetic bead separation and enrichment zone from top to bottom. The magnetic field separation module 300 is the core functional module of the device. Its core principle is gradient magnetic field magnetic separation technology. By providing a gradient magnetic field with adjustable magnetic field strength and range, and utilizing the magnetic response characteristics of magnetic microspheres, it achieves efficient separation and enrichment of magnetic microspheres (target material) with active ingredient-target complex and unbound impurities, solving the problems of low separation efficiency and poor purity of target material and impurities in traditional separation methods.

[0025] The two functional areas operate independently and the materials flow in the forward direction, achieving a graded separation effect of step-by-step impurity removal and directional enrichment.

[0026] An impurity processing component 320 is provided in the impurity separation zone, including a first magnetic field generator 321. The magnetic field of the first magnetic field generator 321 acts on the impurity separation zone to separate a small amount of strong magnetic impurities contained in the magnetic microspheres of the active ingredient-target complex by weak magnetic force. Its working principle is weak magnetic targeted adsorption and separation. By applying a weak magnetic field to the impurity separation area, the difference in magnetic sensitivity between strong magnetic impurities and magnetic microsphere complex is used to target and separate a small amount of strong magnetic impurities contained in the magnetic microspheres of the active ingredient-target complex, thus avoiding strong magnetic impurities from entering the subsequent enrichment process and interfering with the separation and detection of the target analyte. The magnetic bead separation and enrichment zone is equipped with an enrichment component 330 and an export component 340. The enrichment component 330 includes a second magnetic field generator 331, whose magnetic field acts on the magnetic bead separation and enrichment zone to specifically adsorb the target magnetic microsphere complex, causing the magnetic microsphere complex to adhere to the sidewall of the magnetic bead separation and enrichment zone. The export component 340 is used to classify and export the separated magnetic microsphere complex and unbound impurities such as starch and dietary fiber. The enrichment component 330 and the export component 340 work together to achieve the adsorption, enrichment, and classification export of the target analyte. The core of the enrichment component 330 is the second magnetic field generator 331, which applies a strong magnetic field to the magnetic bead separation and enrichment zone using the principle of strong magnetic directional adsorption. This causes the magnetically responsive target magnetic microsphere complex to be specifically adsorbed and attached to the sidewall of the magnetic bead separation and enrichment zone, achieving the initial separation of the target analyte from non-magnetic unbound impurities such as starch and dietary fiber. The export component 340, based on the differences in material phase and magnetic properties, uses structural design to achieve the classified and directional export of the separated magnetic microsphere complex and non-magnetic impurities, avoiding material mixing and ensuring the purity of the raw materials in subsequent purification stages.

[0027] In this embodiment, the sample pretreatment module 100 is used to filter, remove impurities from, and adjust the concentration of the yam extract. The sample pretreatment module 100 includes a storage tank for storing samples, a filtration assembly, a concentration adjustment chamber, and a delivery pump for sample pretreatment. The outlet of the storage tank is connected to the filtration assembly, which includes a microporous membrane layer, an ultrafiltration membrane layer, and an adsorption and impurity removal layer that are sequentially nested together. The outlet of the filtration assembly is connected to the concentration adjustment chamber, which has a built-in concentration sensor and a temperature control plate. The outlet of the concentration adjustment chamber is connected to the magnetic microsphere incubation module 200 via the delivery pump.

[0028] In this embodiment, the magnetic microsphere incubation and binding module 200 is used to load magnetic microspheres with coupled target probes and realize the isothermal incubation and specific binding of magnetic microspheres with active ingredients in yam extract. Its core principle is based on the molecular recognition between target probes and active ingredients in yam, so that the active ingredients specifically bind to the target probes on the magnetic microspheres to form a complex of active ingredients-target probes-magnetic microspheres, providing magnetically responsive target substances for subsequent magnetic field separation. This module consists of a constant-temperature incubation chamber, a magnetic microsphere loading box, a stirring assembly, and a pH adjustment assembly. Each component is designed to optimize molecular binding conditions: the constant-temperature incubation chamber serves as the core reaction vessel, enabling sealed storage of the sample solution after pretreatment. It has a built-in temperature sensor and a heating / cooling element. The temperature sensor monitors the temperature inside the chamber in real time, while the heating / cooling element enables precise temperature control (temperature control accuracy ±0.5℃), providing a suitable constant-temperature environment for specific molecular binding; the magnetic microsphere loading box is a detachable structure installed inside the constant-temperature incubation chamber, facilitating the loading and replacement of magnetic microspheres and cleaning of the chamber, avoiding cross-contamination. The stirring assembly consists of a stirring motor and a spiral stirring paddle that extends into the loading box. The stirring motor drives the spiral stirring paddle to rotate at a low constant speed (adjustable from 0-500 r / min), which ensures that the magnetic microspheres are fully mixed with the sample solution, increases the probability of molecular contact, and improves the binding efficiency. The pH adjustment assembly consists of a storage bottle and a dropper. The end of the dropper extends into the loading box and is equipped with a metering dropper. The storage bottle contains acid / base adjustment solution, and the metering dropper enables the quantitative addition of the adjustment solution. By adjusting the pH value of the reaction system, the molecular conformation of the target probe and the active ingredient of yam is in the optimal binding state, further improving the specific binding efficiency.

[0029] In this embodiment, the elution and purification module 400 is used to perform gradient elution on the enriched magnetic microsphere complex to obtain purified yam active ingredient-target complex. This module consists of an eluent storage component, a gradient elution chamber, and a purification filtration component, forming a continuous gradient elution-deep purification process: The eluent storage component contains at least two storage bottles holding eluents of different concentrations. Each storage bottle is connected to the gradient elution chamber via a quantitative delivery tube. The quantitative delivery tube is equipped with a flow valve. By adjusting the opening and closing degree and flow rate of each flow valve, the quantitative ratio and continuous delivery of eluents of different concentrations are achieved, forming a gradient concentration eluent system. The gradient elution chamber is the core elution reaction vessel, with a built-in ultrasonic oscillation component. The ultrasonic oscillation component applies low-frequency ultrasound (20-40kHz) to ensure sufficient contact between the eluent and the magnetic microsphere complex, accelerating the desorption of non-specific binders and improving elution efficiency. The purification filtration component is located at the outlet of the gradient elution chamber and consists of a nanofiltration membrane layer and an ion exchange resin layer. The nanofiltration membrane layer retains the magnetic microsphere complex in the eluent to prevent the loss of the target substance, while the ion exchange resin layer removes ionic impurities in the eluent, achieving deep purification of the target substance and ultimately obtaining the purified yam active ingredient-target complex.

[0030] In this embodiment, the target analysis and detection module 500 is used to perform qualitative identification and quantitative detection of the target in the eluted complex. High performance liquid chromatography-mass spectrometry (HPLC-MS) is used, which combines the high efficiency of chromatography with the high sensitivity and specificity of mass spectrometry to achieve accurate qualitative and quantitative identification of the target, providing accurate detection data for target research of yam active ingredients.

[0031] This module consists of an injection port, a high-performance liquid chromatography (HPLC) detection unit, a mass spectrometry (MS) detection unit, and a data recording assembly. All components employ a tandem detection design: the injection port is connected to the outlet of the elution and purification module 400, enabling automated quantitative injection of the purified sample with micro-level precision, ensuring repeatability; the HPLC detection unit is a separation system that utilizes the interaction differences between the stationary phase of the chromatographic column and the components in the sample to achieve efficient separation of the active ingredient-target complex from trace impurities, providing a single-component sample stream for subsequent mass spectrometry detection; the mass spectrometry detection unit is... The detection system, connected in series with the high-performance liquid chromatography (HPLC) detection unit, ionizes the target components after chromatographic separation. Qualitative identification of the target is achieved by detecting the mass-to-charge ratio of the ions, while quantitative detection is achieved based on the ion signal intensity. The data recording component consists of a detector and a data storage module. The detector acquires chromatograms and mass spectra in real time during the target detection process. The chromatograms reflect the separation of the target components, while the mass spectra reflect the molecular structure and content information of the target components. The data storage module enables real-time storage, export, and analysis of detection data and spectra, providing data support for subsequent research.

[0032] See attached document Figure 5The enrichment component 330 also includes several arc-shaped ribs 332 fixed to the inner wall of the magnetic bead separation enrichment zone, and the several arc-shaped ribs 332 are arranged in a circular array. Its core design principle is to increase the effective magnetic adsorption area. Through the protruding structure of the arc-shaped ribs 332, the specific surface area of ​​the side wall of the magnetic bead separation enrichment zone is increased, so that more magnetic microsphere complexes can be adsorbed by the magnetic field, thereby improving the enrichment efficiency of the target analyte.

[0033] The separation container 310 is externally fixedly connected to a motor drive group 333 for the annular displacement of the second magnetic field generator 331. The magnetic field of the second magnetic field generator 331 covers the entire height of the arc-shaped ribs 332, and the liquid level in the magnetic bead separation enrichment zone is lower than the middle of the arc-shaped ribs 332. When the second magnetic field generator 331 moves in annular motion, it drives the magnetic bead complex attached to the side wall of the magnetic bead separation enrichment zone to move upward along the support surface of several arc-shaped ribs 332, thereby increasing the adhesion surface of the magnetic bead complex. This design employs a dynamic magnetic field scanning technology. The motor drive unit 333 drives the second magnetic field generator 331 to perform a uniform circular motion along the outer wall of the separation container 310. The magnetic field of the second magnetic field generator 331 covers the entire height of the arc-shaped rib 332, ensuring that the magnetic field fully covers the surface of the arc-shaped rib 332. At the same time, the liquid level in the magnetic bead separation enrichment zone is lower than the middle of the arc-shaped rib 332. Its function is to enable the magnetic microsphere composite to move upward along the support surface of the arc-shaped rib 332 under the action of the magnetic field, further increasing the adhesion surface of the magnetic bead composite, reducing the loss of the target material, and improving the enrichment efficiency.

[0034] See attached document Figure 5 The export component 340 includes an impurity discharge pipe 341 and a complex discharge pipe 342 connected to the interior of the magnetic bead separation and enrichment zone; it adopts a dual-pipeline classification export design, based on the differences in magnetic properties and physical distribution between the target material and the impurities, to achieve directional separation and export of non-magnetic impurities and magnetic microsphere complexes.

[0035] Impurity discharge pipe 341 is fixedly connected to the bottom of separation container 310; its core function is to remove non-magnetic unbound impurities (such as starch and dietary fiber) from the magnetic bead separation enrichment area. Since non-magnetic impurities do not have magnetic responsiveness, they are deposited at the bottom of separation container 310 under the action of gravity, and the non-magnetic impurities are continuously removed through impurity discharge pipe 341.

[0036] The top end of the complex discharge pipe 342 is fixedly connected to a funnel-shaped support plate 343, which is located below the magnetic bead separation and enrichment zone. An annular gap is reserved between the funnel-shaped support plate 343 and the separation container 310 for the downward discharge of impurities. The magnetic field of the second magnetic field generator 331 acts on the annular gap to precisely adsorb the weak magnetic bead complex remaining in the liquid flowing down through the annular gap. The annular gap serves as a flow channel for non-magnetic impurities. Simultaneously, the magnetic field of the second magnetic field generator 331 acts within this annular gap. Its core principle is magnetic trapping technology. By fully covering the annular gap with a magnetic field, it precisely adsorbs the weakly magnetic microsphere complex remaining in the liquid flowing down through the annular gap, preventing the target material from being lost with the non-magnetic impurities and further improving the recovery rate of the target material.

[0037] See attached document Figure 5 The bucket-shaped support plate 343 includes a rubber part T1 and a rigid part T2. The rigid part T2 is fixed to the inside of the separation container 310 by a bracket. The bucket-shaped support plate 343 adopts a composite structure design, consisting of a rubber part T1 and a rigid part T2. The rigid part T2 is fixed to the inside of the separation container 310 by a bracket, providing structural support for the bucket-shaped support plate 343 and ensuring its positional stability within the separation container. The rubber part T1 is an elastic and deformable structure, realizing the support and guidance of materials. The combination of the two takes into account both structural stability and functional adjustability.

[0038] An expandable sealing airbag 344 is embedded in the outer edge of the rigid part T2. The expansion of the sealing airbag 344 seals the annular gap, allowing the magnetic bead composite on the bucket-shaped support plate 343 to be discharged separately through the composite discharge pipe 342. The core function of the sealing airbag 344 is to achieve controllable sealing of the annular gap. When it is necessary to export the magnetic microsphere composite, the sealing airbag 344 inflates and completely seals the annular gap between the bucket-shaped support plate 343 and the separation container 310, cutting off the flow channel of non-magnetic impurities. This allows the magnetic microsphere composite on the bucket-shaped support plate 343 to be exported separately through the composite discharge pipe 342, avoiding impurity contamination and ensuring the purity of the target material. The bottom end of the complex discharge pipe 342 extends to the bottom of the separation container 310, and a sealing sleeve is provided at the penetration part of the separation container 310 to achieve sealing when the complex discharge pipe 342 moves up and down, preventing material leakage. A regulating cylinder 345 for adjusting the complex discharge pipe 342 up and down is fixedly connected to the separation container 310. The regulating cylinder 345 is a power component that drives the complex discharge pipe 342 to make precise up and down linear movements. Through the up and down displacement of the complex discharge pipe 342, the linkage control between the shape change of the bucket-shaped support plate 343 and the opening and closing of the complex discharge pipe 342 is realized. Impurity removal stage: The regulating cylinder 345 drives the complex discharge pipe 342 to move upward. The top of the complex discharge pipe 342 is tightly fitted with the sealing plug 346 fixed in the magnetic bead separation and enrichment area, thus sealing the complex discharge pipe 342 and preventing non-magnetic impurities from entering. At the same time, the upward displacement of the complex discharge pipe 342 causes the rubber part T1 of the bucket-shaped support plate 343 to convex upward, forming a guide surface. This allows the non-magnetic impurities in the magnetic bead separation and enrichment area to be deposited downward through the annular gap along the guide surface of the rubber part T1 under the action of gravity, and finally discharged by the impurity discharge pipe 341, thus achieving efficient discharge of non-magnetic impurities. Complex export stage: Adjusting cylinder 345 drives complex discharge pipe 342 to move downward, sealing plug 346 loses its blockage of complex discharge pipe 342, making complex discharge pipe 342 conductive; at the same time, the downward displacement of complex discharge pipe 342 causes the rubber part T1 of bucket-shaped support plate 343 to be concave, forming a support surface to support the magnetic microsphere complex after magnetic field desorption; at this time, sealing airbag 344 inflates to seal the annular gap, and the magnetic bead separation enrichment area is demagnetized, causing the magnetic microsphere complex attached to the side wall of the separation area to fall off under the action of gravity and deposit on the concave support surface of bucket-shaped support plate 343, and finally be directionally exported through complex discharge pipe 342, realizing the lossless discharge of the target material.

[0039] Example 2: The difference from Example 1 is that; See attached document Figure 5 and Figure 6 The separation container 310 is fixedly connected to a pressure cylinder 347 by a bracket. The pressure cylinder 347 is slidably sleeved on the outer surface of the compound discharge pipe 342 through a sealing sleeve. The inside of the pressure cylinder 347 is connected to the inside of the sealing airbag 344 through a pressure pipe. A piston plate 348 is fixed on the outer surface of the compound discharge pipe 342. When the piston plate 348 moves downward through the compound discharge pipe 342 into the pressure cylinder 347, it pressurizes the gas inside the pressure cylinder 347, causing the sealing airbag 344 to expand.

[0040] The core design principle of this structure is mechanical linkage pressurization technology. It uses the vertical displacement of the compound discharge pipe 342 as a power source to realize the automatic inflation of the sealing airbag 344. There is no need to set up additional pressurization equipment such as an air pump, which simplifies the device structure and realizes the synchronous linkage of mechanical action and sealing action. Specific working principle and function: When the regulating cylinder 345 drives the composite discharge pipe 342 to move downward, the composite discharge pipe 342 drives the piston plate 348 to move downward synchronously. When the piston plate 348 moves downward into the pressure cylinder 347, it compresses the sealed gas inside the pressure cylinder 347, creating high-pressure gas inside the pressure cylinder 347. The high-pressure gas quickly enters the sealing bladder 344 through the pressure pipe, causing the sealing bladder 344 to automatically expand, achieving rapid and tight sealing of the annular gap. This structure achieves synchronous downward displacement of the composite discharge pipe 342 and expansion and sealing of the sealing bladder 344, ensuring the sealing performance of the magnetic microsphere composite export stage, preventing leakage of the target material, simplifying the control process of the device, and improving the automation level of the device.

[0041] Example 3: The difference from Example 1 is that; See attached document Figure 4 The impurity treatment component 320 also includes a feeding pipe 322 connected to the top of the container 310 via a support, and a dispersion hopper Q is fixedly connected to the bottom end of the feeding pipe 322. A gap is reserved between the outer edge of the dispersion hopper Q and the inner wall of the impurity separation zone, which is used to disperse the compound inside the feeding pipe 322 to the inner surface of the impurity separation zone, so that the compound adheres to the inner wall of the impurity separation zone and flows down. Then, the magnetic field of the first magnetic field generator 321 causes the strong magnetic impurities in the compound to remain on the inner wall of the impurity separation zone, thus forming separation. This structure adopts thin film flow distribution technology. Its core principle is to disperse the magnetic microsphere compound transported through the feeding pipe 322 into a thin film liquid flow along the inner wall of the impurity separation zone, thereby increasing the contact area and contact time between the compound and the weak magnetic field generated by the first magnetic field generator 321, and improving the separation efficiency of strong magnetic impurities. The magnetic field strength of the first magnetic field generator 321 is 0.1~0.2T, which is used to adsorb strong magnetic impurities individually without adsorbing magnetic microspheres.

[0042] Specific working principle and function: The injection pipe 322 conveys the magnetic microsphere composite to the dispersion hopper Q. The dispersion hopper Q disperses the composite in all directions through its conical structure, so that the composite flows downward in a thin film along the inner surface of the impurity separation zone through the gap. This flow mode allows each particle in the composite to fully contact the weak magnetic field of the inner wall of the impurity separation zone. The first magnetic field generator 321 applies a weak magnetic field of 0.1~0.2T. The strength of this magnetic field is precisely controlled so that it only targets and adsorbs strong magnetic impurities in the composite without adsorbing the magnetic microsphere composite, thus avoiding the loss of the target material. Under the adsorption of a weak magnetic field, strong magnetic impurities remain on the inner wall of the impurity separation zone and are effectively separated from the magnetic microsphere complex that flows down in a thin film. This removes the strong magnetic impurities from the complex and ensures the magnetic field separation accuracy of the subsequent magnetic bead separation and enrichment process.

[0043] Example 4: The difference from Example 3 is that; See attached document Figure 4 The inner wall of the impurity separation zone is provided with an annular receiving groove 323, and the opening of the receiving groove 323 is located above the gap opening. The first magnetic field generator 321 is axially slidably connected to the inner surface of the separation container 310, and its height is adjusted by an electric telescopic rod 324 fixed on the separation container 310. The upward displacement of the first magnetic field generator 321 drives the strong magnetic impurities attached to the impurity separation zone to move upward. A magnetic isolation sleeve 325 is installed in the lining of the impurity separation zone. The magnetic isolation sleeve 325 is made of a magnetic shielding material with high magnetic permeability and has a magnetic field shielding effect. It can effectively isolate the weak magnetic field generated by the first magnetic field generator 321 and isolate the magnetic field of the first magnetic field generator 321. When the strong magnetic impurities move to the opening of the receiving groove 323, they lose their magnetic adsorption and automatically enter the receiving groove 323.

[0044] The core design principle of this structure is magnetic field-oriented drive and shielding desorption technology, which realizes the automated separation, collection and storage of strong magnetic impurities, avoids the accumulation of strong magnetic impurities on the inner wall of the impurity separation zone, and ensures the continuous and efficient operation of the impurity separation zone. The specific working principle and function are divided into three stages: Adsorption and separation stage: The first magnetic field generator 321 is located at the lower part of the impurity separation zone under the action of the electric telescopic rod 324, and a weak magnetic field of 0.1~0.2T is applied. The magnetic microsphere composite forms a thin film liquid flow along the inner wall through the dispersion hopper Q. The strong magnetic impurities are adsorbed on the inner wall of the impurity separation zone under the action of the weak magnetic field, thus achieving separation from the target.

[0045] Magnetic field directional drive stage: When the amount of strong magnetic impurities adsorbed on the inner wall of the impurity separation zone reaches a certain amount, the electric telescopic rod 324 drives the first magnetic field generator 321 to move upward at a uniform speed. The weak magnetic field generated by the first magnetic field generator 321 moves upward synchronously. The magnetic field adsorption force drives the strong magnetic impurities attached to the impurity separation zone to move upward synchronously, thereby realizing the directional transport of strong magnetic impurities.

[0046] Shielding, release, and collection stage: When strong magnetic impurities move upward with the magnetic field to the opening of the receiving tank 323, the magnetic isolation sleeve 325 is installed in the impurity separation area around the receiving tank 323. The magnetic isolation sleeve 325 effectively shields the weak magnetic field of the first magnetic field generator 321, causing the magnetic field strength at the opening of the receiving tank 323 to drop to zero. The strong magnetic impurities lose their magnetic attraction and automatically fall into the receiving tank 323 under the action of gravity, realizing the automated collection and storage of strong magnetic impurities.

[0047] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A magnetic microsphere separation and analysis device for target extraction of active ingredients from yam, characterized in that, It includes a sample pretreatment module, a magnetic microsphere incubation and bonding module, a magnetic field separation module, an elution and purification module, and a target analysis and detection module, which are connected sequentially through a sealed delivery pipeline. The magnetic field separation module is used to provide a gradient-adjustable magnetic field to achieve the separation and enrichment of magnetic microspheres bound with active ingredient-target complexes and the separation of unbound impurities. The magnetic field separation module includes a cylindrical separation container, and the interior of the separation container is provided with an impurity separation zone and a magnetic bead separation and enrichment zone from top to bottom. An impurity processing component is provided in the impurity separation zone, including a first magnetic field generator. The magnetic field of the first magnetic field generator acts on the impurity separation zone to separate a small amount of strong magnetic impurities contained in the magnetic microspheres of the active ingredient-target complex by weak magnetic force. The magnetic bead separation and enrichment area is equipped with an enrichment component and an export component; The enrichment component includes a second magnetic field generator. The magnetic field of the second magnetic field generator acts on the magnetic bead separation and enrichment zone to specifically adsorb the target magnetic microsphere complex, so that the magnetic microsphere complex is attached to the sidewall of the magnetic bead separation and enrichment zone. The export component is used to classify and export the separated magnetic microsphere complex and unbound impurities such as starch and dietary fiber.

2. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The sample pretreatment module is used to filter, remove impurities from, and adjust the concentration of the yam extract. The sample pretreatment module includes a storage tank for storing samples, a filtration assembly for sample pretreatment, a concentration adjustment chamber, and a delivery pump.

3. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The magnetic microsphere incubation and binding module is used to load magnetic microspheres that are coupled with target probes, and to achieve constant temperature incubation and specific binding of magnetic microspheres with active ingredients in yam extract. The magnetic microsphere incubation module includes a constant-temperature incubation chamber for storing the pretreatment liquid, a loading box for injecting magnetic microspheres into the constant-temperature incubation chamber, and a stirring assembly and a pH adjustment assembly for processing the mixture.

4. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The elution and purification module is used to perform gradient elution on the enriched magnetic microsphere complex to obtain purified yam active ingredient-target complex. The elution and purification module includes an eluent storage component, a gradient elution chamber, and a purification filtration component.

5. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The target analysis and detection module is used to perform qualitative and quantitative identification of targets in the eluted complex. The target analysis and detection module includes an injection port, a high-performance liquid chromatography (HPLC) detection unit, a mass spectrometry (MS) detection unit, and a data recording component. The injection port is connected to the outlet of the elution and purification module. The HPLC detection unit and the MS detection unit are connected in series. The data recording component includes a detector and a data storage module. The detector is used to collect chromatograms and mass spectra of the target detection.

6. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The enrichment component also includes several arc-shaped ribs fixed to the inner wall of the magnetic bead separation enrichment area, and the several arc-shaped ribs are arranged in a circular array. The separation container is externally fixedly connected to a motor drive group for the annular displacement of the second magnetic field generator. The magnetic field of the second magnetic field generator covers the entire height of the arc-shaped ribs, and the liquid level in the magnetic bead separation enrichment zone is lower than the middle of the arc-shaped ribs. When the second magnetic field generator moves in annular motion, it drives the magnetic bead complex attached to the side wall of the magnetic bead separation enrichment zone to move upward along the support surface of several arc-shaped ribs, thereby increasing the adhesion surface of the magnetic bead complex.

7. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The export component includes an impurity discharge tube and a complex discharge tube connected to the interior of the magnetic bead separation and enrichment zone; The impurity discharge pipe is fixedly connected to the bottom of the separation container; The top end of the complex discharge pipe is fixedly connected to a funnel-shaped support plate, which is located below the magnetic bead separation and enrichment zone. An annular gap is reserved between the funnel-shaped support plate and the separation container for the downward discharge of impurities. The magnetic field of the second magnetic field generator acts on the annular gap to precisely adsorb the weak magnetic bead complex residue in the liquid flowing down through the annular gap.

8. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 7, characterized in that, The bucket-shaped support plate includes a rubber part T1 and a rigid part T2, wherein the rigid part T2 is fixed to the inside of the separation container by a bracket; An expandable sealing airbag is embedded in the outer edge of the rigid part T2, which is used to seal the annular gap by expanding the sealing airbag, so that the magnetic bead compound on the bucket-shaped support plate can be discharged separately through the compound discharge pipe. The bottom end of the compound discharge pipe extends to the bottom of the separation container, and a sealing sleeve is provided at the part through which it penetrates the separation container. An adjusting cylinder for adjusting the compound discharge pipe up and down is fixedly connected to the separation container. When the complex discharge tube moves upward, the top of the complex discharge tube is sealed with the sealing plug fixed in the magnetic bead separation and enrichment area, and the rubber part T1 of the bucket-shaped support plate is in an upward convex state, so that the non-magnetic impurities in the magnetic bead separation and enrichment area are discharged downward through the annular gap. When the composite discharge tube moves downward, the sealing plug loses its blockage of the composite discharge tube, and the rubber part T1 of the bucket-shaped support plate is concave. The sealing airbag seals the annular gap and demagnetizes the magnetic bead separation enrichment area, allowing the separated magnetic microsphere composite to be discharged through the composite discharge tube.

9. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 1, characterized in that, The impurity treatment component also includes a feeding pipe connected to the top of the container via a support, and a dispersion hopper Q is fixedly connected to the bottom end of the feeding pipe. The outer edge of the dispersion hopper Q and the inner wall of the impurity separation zone are reserved with a gap for dispersing the complex inside the feeding pipe to the inner surface of the impurity separation zone, so that the complex adheres to the inner wall of the impurity separation zone and flows down. Then, the magnetic field of the first magnetic field generator causes the strong magnetic impurities in the complex to remain on the inner wall of the impurity separation zone, thus forming a separation. The magnetic field strength of the first magnetic field generator is 0.1~0.2T, and it is used to adsorb strong magnetic impurities individually.

10. The magnetic microsphere separation and analysis device for target extraction of active ingredients from yam according to claim 9, characterized in that, The inner wall of the impurity separation zone is provided with an annular receiving groove, and the opening of the receiving groove is located above the gap opening. The first magnetic field generator is axially slidably connected to the inner surface of the separation container, and its height is adjusted by an electric telescopic rod fixed to the separation container. The upward displacement of the first magnetic field generator drives the strong magnetic impurities attached to the impurity separation zone to move upward. A magnetic isolation sleeve is installed in the lining of the impurity separation zone to isolate the magnetic field of the first magnetic field generator, so that when the strong magnetic impurities move to the opening of the receiving tank, they lose their magnetic attraction and automatically enter the receiving tank.

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