Cytochrome c mutant and its application in synthesis of organic germanium compounds
The preparation of chiral organogermanium compounds in an aqueous phase at room temperature using a cytochrome C mutant catalyst solves the problem of preparing single chiral organogermanium compounds in existing technologies, realizing an efficient, green, and environmentally friendly synthesis method with high chemical purity and few byproducts.
CN122404536APending Publication Date: 2026-07-17SHANDONG RES INST OF IND TECH
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANDONG RES INST OF IND TECH
- Filing Date
- 2026-06-11
- Publication Date
- 2026-07-17
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Figure CN122404536A_ABST
Abstract
本发明属于酶催化技术领域，涉及一种细胞色素C突变体及其在合成有机锗化合物中的应用。该突变体的氨基酸序列如SEQ ID NO.4所示。本发明还进一步提供了编码所述细胞色素C突变体的基因、包含所述基因的重组质粒、表达所述细胞色素C突变体的重组微生物。以及该突变体及重组微生物在合成手性有机锗化合物中的应用。本发明还提供一种使用工程化的细胞色素C突变体不对称合成手性有机锗化合物的方法，可以催化有机锗烷类化合物与重氮化合物构建碳‑锗（C–Ge）键。本发明通过使用极低载量的生物催化剂即可在常温、水相缓冲溶液等温和条件下高效进行，并且具有广泛的底物适用性。
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