Pseudorabies virus nanoparticle antigen and preparation method and application thereof

By covalently linking the protective antigen protein of pseudorabies virus to the scaffold protein of nanoparticles, a pseudorabies subunit nanoparticle vaccine was prepared, which solved the problem of insufficient immune protection of existing vaccines against variant strains and achieved efficient and safe immunization and differential diagnostic capabilities.

CN122404565APending Publication Date: 2026-07-17CHINA ANIMAL HUSBANDRY IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA ANIMAL HUSBANDRY IND
Filing Date
2025-01-15
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing pseudorabies vaccines are not very effective in protecting against viral variants, and it is difficult to distinguish between vaccine-immunized and wild-type virus infection through serological differential diagnosis. Their safety and immunogenicity need to be improved.

Method used

The protective antigen proteins gB, gC, and gD of pseudorabies virus were covalently linked in vitro to the nanoparticle backbone protein mi3 to form nanoparticle antigens, and a pseudorabies subunit nanoparticle vaccine was prepared. Combined with ISA 206VG adjuvant, the immune response was enhanced.

Benefits of technology

It enhances immunogenicity, induces high-level antibody responses, exhibits good safety and persistence, and can differentiate between vaccine-immunized individuals and wild-type virus infections through antibody differential diagnosis, effectively preventing and controlling the spread of pseudorabies.

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Abstract

本发明公开了一种伪狂犬病病毒纳米颗粒抗原及其制备方法和应用。本发明的自组装纳米颗粒包含a)和b)所述的亚组分：a)与SpyTag融合表达的伪狂犬病病毒gB‑SP、gC‑SP或者gD‑SP蛋白；b)与SpyCatcher融合表达的自组装纳米颗粒骨架蛋白SpyCatcher‑mi3。与常规的蛋白组分亚单位疫苗相比，本发明提供的gB‑mi3、gC‑mi3和gD‑mi3纳米颗粒抗原制备成的亚单位纳米颗粒疫苗可显著提高抗原蛋白的免疫原性，在免疫靶动物仔猪后能够产生高水平的特异性ELISA抗体和针对伪狂犬病病毒的中和抗体，刺激机体产生强烈的体液免疫反应，对伪狂犬病病毒感染相关疾病的预防和控制具有重要的意义和应用前景。
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Citation Information

Patent Citations

  • Porcine pseudorabies virus gene deleted strain, as well as construction method and application thereof

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