Anti-wrinkle and firming composition suitable for multiple skin types, and preparation method and application thereof

Abstract

The application discloses an anti-wrinkle and firming composition suitable for multiple skin types and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition contains, in mass parts, 2.5-9.5 parts of an active functional component, 5-10 parts of a targeted delivery carrier system and cosmetically acceptable adjuvants. The active functional component is composed of ginsenoside Rb1, green tea extract-white resveratrol compound and asiaticoside-dipotassium glycyrrhizinate compound, and the targeted delivery carrier system is a transdermal peptide modified liposome and a natural nano-structured lipid carrier. The application constructs a double-targeted delivery system according to different skin aging mechanisms and skin characteristics, realizes single formula adaptation to multiple skin types, improves the transdermal efficiency of active ingredients and the targeted enrichment effect on the dermis, has no carrier residue, and multiple components synergistically play the effects of anti-wrinkle and firming, soothing and protection, is safe, and can be widely applied to the preparation of anti-aging skin care products.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and firming composition suitable for multiple skin types, its preparation method, and its application. Background Technology

[0002] Skin aging is a complex process resulting from the combined effects of endogenous physiological aging and exogenous environmental damage. Its core manifestations include the degradation and loss of collagen and elastin fibers in the dermis, impaired skin barrier function, and visible signs such as deepening wrinkles, sagging skin, decreased elasticity, and enlarged pores. As consumers' skincare needs become more refined, anti-aging has become a core segment of the skincare market. Simultaneously, consumers are demanding higher standards for product suitability for different skin types, precise efficacy, and safety.

[0003] Existing anti-aging skincare compositions still suffer from three major technological challenges, failing to meet the diverse needs of the market: First, there is a serious lack of skin type compatibility; fixed formula systems cannot meet the diverse needs of various skin types. Most existing anti-aging products use a single, fixed formula designed only for a specific skin type: highly moisturizing formulas for dry / mature skin can easily cause clogged pores, breakouts, and excessive oiliness on oily skin, exacerbating sebum secretion disorders; refreshing, oil-controlling formulas for oily skin can lead to insufficient moisturizing power on dry skin, exacerbating dryness and flaking, and further deepening fine lines; while sensitive skin, due to a damaged skin barrier, has extremely low tolerance to irritating ingredients, and most traditional anti-aging ingredients (such as high-concentration retinol, fruit acids, and virgin vitamin C) can easily trigger allergic reactions such as redness, stinging, and itching. Existing formulas cannot simultaneously achieve multi-scenario adaptability—moisturizing and anti-wrinkle for dry skin, oil-controlling and firming for oily skin, and soothing and anti-aging for sensitive skin—resulting in a severely limited target audience and a poor user experience.

[0004] Secondly, the transdermal delivery efficiency of active ingredients is low, failing to achieve precise targeted delivery to the dermis. The stratum corneum is a natural physiological barrier. Most anti-aging active ingredients have large molecular weights and poor hydrophilicity-hydrophobicity matching, making it difficult for them to penetrate the stratum corneum and reach the dermis to exert their effects through passive diffusion alone. In traditional formulas, more than 90% of the active ingredients remain in the epidermis and are lost through sweat and stratum corneum metabolism, resulting in extremely low bioavailability. Existing technologies often use carriers such as ordinary liposomes and nanoemulsions to improve transdermal permeability, but there are still significant drawbacks: ordinary liposomes have poor storage stability, are prone to leakage of active ingredients and particle aggregation, and lack targeting, failing to guide the precise enrichment of active ingredients in the dermis; traditional nanostructured lipid carriers (NLCs) mostly use synthetic lipids as raw materials, have poor biocompatibility, cannot be completely metabolized by the skin, and are prone to residues in the skin, which can damage the skin barrier with long-term use, especially being extremely unfriendly to sensitive skin; at the same time, existing carriers are mostly single, universal systems, unable to be differentiated according to the physicochemical properties of different active ingredients and the skin barrier characteristics of different skin types, failing to achieve synergistic and precise delivery of multiple active ingredients.

[0005] Third, the anti-aging effects lack synergy, and the utilization rate of active ingredients is extremely low. Existing anti-aging formulas mostly consist of a single active ingredient or a simple, crude accumulation of ingredients, failing to precisely target the aging mechanisms of different skin types: the core aging cause for dry / mature skin is decreased collagen synthesis and moisture loss due to skin barrier damage, requiring collagen promotion, strong hydration, and barrier repair; the core aging cause for oily skin is collagen degradation due to oxidative stress and enlarged, loose pores due to sebum secretion disorders, requiring strong antioxidant effects, oil control, and pore tightening; the core aging cause for sensitive skin is chronic inflammation caused by barrier damage, with inflammatory factors continuously accelerating collagen degradation, leading to premature skin aging, requiring soothing and anti-inflammatory effects, barrier repair, and gentle anti-aging. Existing formulas do not combine differentiated active ingredients with suitable targeted delivery systems, failing to achieve precise release and target enrichment of active ingredients for different skin types. This results in the active ingredients failing to exert synergistic effects, significantly reducing anti-wrinkle and firming efficacy, and even causing skin discomfort due to ingredient incompatibility with skin type.

[0006] Therefore, developing an anti-wrinkle and firming composition that can be adapted to various skin types such as dry skin, oily skin, sensitive skin, and mature skin, and achieve precise targeted delivery of active ingredients to the dermis, no carrier residue, and synergistic effect of multiple ingredients, is a technical problem that urgently needs to be solved in this field, and has extremely high market value and technical significance. Summary of the Invention

[0007] The purpose of this invention is to overcome the aforementioned deficiencies of the prior art and provide an anti-wrinkle and firming composition suitable for multiple skin types, along with its preparation method and application. This invention designs differentiated combinations of active ingredients and a dual-target delivery carrier system tailored to the aging mechanisms and characteristics of different skin types. This allows a single formulation to meet the needs of multiple skin types, significantly improving the transdermal penetration rate and dermal layer targeting enrichment efficiency of active ingredients. It leaves no carrier residue, achieves synergistic effects from multiple components, demonstrates significant anti-wrinkle and firming efficacy, and is highly safe and non-irritating, thus addressing the core pain points of the prior art.

[0008] The objective of this invention can be achieved through the following technical solutions: An anti-wrinkle and firming composition suitable for multiple skin types, comprising, by weight, the following components: 2.5-9.5 parts of active functional component, 5-10 parts of targeted delivery carrier system, and 80.5-92.5 parts of cosmetically acceptable excipients; wherein the active functional component includes 2.0-4.0 parts of ginsenoside Rb1, 1.5-3.0 parts of green tea extract-resveratrol complex, and 1.0-2.5 parts of asiaticoside-dipotassium glycyrrhizate complex; the targeted delivery carrier system includes 3.0-6.0 parts of transdermal peptide-modified liposomes and 2.0-4.0 parts of natural nanostructured lipid carriers.

[0009] Furthermore, in the green tea extract-resveratrol complex, the mass ratio of green tea extract to resveratrol is (1-3):1, preferably 2:1. The green tea extract is prepared by water extraction and alcohol precipitation of green tea, wherein the content of tea polyphenols is ≥98% and the content of EGCG is ≥50%, ensuring the stability of antioxidant activity.

[0010] Furthermore, in the asiaticoside-dipotassium glycyrrhizate complex, the mass ratio of asiaticoside to dipotassium glycyrrhizate is (1-2):1, preferably 1:1. The purity of the asiaticoside is ≥95%, ensuring the consistency of its repair and anti-aging activities.

[0011] Furthermore, in the transdermal peptide-modified liposomes, the transdermal peptide used for modification is at least one of TAT peptide, RDP peptide, and TD-1 peptide, preferably TD-1 peptide. TD-1 peptide is a short peptide composed of 11 amino acids, which can gently open temporary channels in the skin barrier by specifically binding to keratin in the stratum corneum. It is non-irritating and suitable for the transdermal needs of sensitive and dry skin. At the same time, it has extremely strong dermal targeting, which can guide liposomes to accurately accumulate around dermal fibroblasts.

[0012] Furthermore, the lipid membrane material of the transdermal peptide-modified liposome is at least one of natural phospholipids, cholesterol, and polyethylene glycol succinate (VE). The natural phospholipid is at least one of soybean lecithin, egg yolk lecithin, and hydrogenated lecithin, preferably hydrogenated lecithin. Hydrogenated lecithin has strong stability and good biocompatibility, can replenish the lipids required by the skin barrier, repair damaged barriers, and is suitable for the skin characteristics of dry and sensitive skin.

[0013] Furthermore, the lipid material of the natural nanostructured lipid carrier (natural NLC) is a mixture of solid lipids and liquid lipids. The solid lipid is at least one of shea butter, cocoa butter, and beeswax, and the liquid lipid is at least one of jojoba oil, sweet almond oil, and grapeseed oil. The mass ratio of solid lipids to liquid lipids is (2-4):1, preferably 3:1. The natural NLC used in this invention is made entirely from natural edible lipids, without the addition of any synthetic lipids. It can be completely metabolized by the skin without residue, exhibits excellent biocompatibility, and has a refreshing and non-greasy skin feel. It can regulate sebum secretion and is suitable for the skin care needs of oily skin. Its disordered lattice structure can significantly improve the encapsulation rate and storage stability of active ingredients, preventing leakage of active ingredients.

[0014] Furthermore, the cosmetic excipients acceptable to the cosmetic include 5-20 parts of moisturizer, 2-10 parts of emollient, 1-5 parts of emulsifier, 0.1-2 parts of thickener, 0.05-1 part of preservative, and deionized water to make up to 100 parts.

[0015] The moisturizer is at least one of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, panthenol, and betaine; the emollient is at least one of jojoba oil, squalane, lanolin, and caprylic / capric triglyceride; the emulsifier is at least one of glyceryl stearate citrate, cetearyl alcohol, Tween 80, and Span 60; the thickener is at least one of carbomer, xanthan gum, and hydroxyethyl cellulose; and the preservative is at least one of phenoxyethanol, ethylhexylglycerin, and p-hydroxyacetophenone. All of these are mild and non-irritating preservatives commonly used in cosmetics, suitable for sensitive skin.

[0016] The present invention also provides a method for preparing the above-mentioned anti-wrinkle and firming composition suitable for multiple skin types, comprising the following steps: Preparation of S1 drug delivery system: transdermal peptide-modified liposomes loaded with ginsenoside Rb1, natural nanostructured lipid carriers loaded with green tea extract-resveratrol complex, and transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex were prepared respectively. S2 Aqueous Phase Preparation: Add humectant, thickener, and deionized water to the aqueous phase pot, heat to 75-85℃, stir at 200-400 rpm until completely dissolved, homogenize at 3000 rpm for 1-3 minutes to obtain the aqueous phase, and keep warm at 75-85℃ for later use. S3 oil phase preparation: Add emollient and emulsifier to oil phase pot, heat to 75-85℃, stir at 200-400rpm until completely dissolved and homogeneous to obtain oil phase, keep warm at 75-85℃ for later use. S4 Emulsification and Mixing: Under homogenization conditions of 75-85℃ and 3000rpm, slowly add the oil phase to the aqueous phase and homogenize and emulsify for 3-8 minutes to obtain an emulsion system; turn on the stirrer to cool down, and when the temperature drops to 40-45℃, add the three drug delivery systems prepared in step S1 and stir evenly at 200-400rpm; continue to cool down to 38-40℃, add the preservative, and stir evenly; continue to cool down to room temperature (20-25℃), and discharge the material to obtain an anti-wrinkle and firming composition suitable for multiple skin types.

[0017] Further, in step S1, the preparation method of the transdermal peptide-modified liposomes carrying ginsenoside Rb1 is as follows: The lipid membrane material and ginsenoside Rb1 are dissolved in anhydrous ethanol and stirred in a water bath at 30-40°C until completely dissolved to obtain an organic phase; the organic phase is transferred to a rotary evaporator and evaporated under reduced pressure at 38-42°C and -0.08~-0.1 MPa to remove the anhydrous ethanol, forming a uniform and transparent lipid film on the inner wall of the flask; phosphate-buffered saline (PBS) at pH 7.4 is added to the flask. The liposome suspension was obtained by hydration in a water bath at 38-42℃ for 20-40 min. The liposome suspension was then placed in a probe-type sonicator and sonicated for 8-12 min under ice bath conditions (power 150-250W, 3s sonication, 5s interval) to obtain the initial liposomes. A transdermal peptide was added to the initial liposomes and incubated at 35-38℃ for 1.5-2.5 h to allow the transdermal peptide to bind to the liposome surface through hydrophobic interactions, completing the modification and yielding transdermal peptide-modified liposomes loaded with ginsenoside Rb1. The average particle size was 100-150 nm, the zeta potential was -20 to -30 mV, and the encapsulation efficiency was ≥90%.

[0018] Further, in step S1, the preparation method of the transdermal peptide-modified liposomes loaded with the asiaticoside-dipotassium glycyrrhizate complex is the same as that of the transdermal peptide-modified liposomes loaded with ginsenoside Rb1, except that the active ingredient is replaced with the asiaticoside and dipotassium glycyrrhizate complex. The average particle size was measured to be 100-140 nm, the zeta potential was -20 to -30 mV, and the encapsulation efficiency was ≥88%.

[0019] Further, in step S1, the preparation method of the natural NLC loaded with green tea extract-resveratrol complex is as follows: The mixture of solid lipids, liquid lipids, green tea extract, and resveratrol is heated in a water bath at 75-85℃ until completely melted and stirred evenly to obtain an oil phase. An emulsifier is added to deionized water and heated in a water bath at 75-85℃ until completely dissolved to obtain an aqueous phase. Under stirring conditions of 75-85℃ and 2000-4000 rpm, the oil phase is slowly added dropwise to the aqueous phase. After the addition is complete, stirring continues for 20-40 minutes to obtain a pre-emulsion. The pre-emulsion is placed in a probe-type ultrasonic instrument and ultrasonically treated under ice bath conditions for 5-10 minutes (power 150-250W, 3s ultrasonic treatment, 5s interval). It is then rapidly cooled to room temperature in an ice-water bath to obtain the natural NLC loaded with green tea extract-resveratrol complex. Tests showed that the average particle size was 80-120 nm, the zeta potential was -25 to -35 mV, and the encapsulation efficiency was ≥92%.

[0020] This invention also provides the application of the above-mentioned anti-wrinkle and firming composition suitable for multiple skin types in the preparation of anti-wrinkle, firming, soothing and repairing skin care products. The skin care products include various dosage forms such as serums, lotions, creams, toners, and masks, with no dosage form limitations.

[0021] The beneficial effects of this invention are: 1. This invention precisely designs a combination system of "differentiated active ingredients + suitable carriers" to address the different aging mechanisms and skin barrier characteristics of dry / mature skin, oily skin, and sensitive skin: For dry / mature skin, transdermal peptide-modified liposomes encapsulate ginsenoside Rb1, which promotes collagen production and reduces wrinkles while replenishing the lipids needed by the skin barrier, enhancing moisturizing power, and repairing the damaged barrier; For oily skin, natural NLC encapsulates green tea extract-resveratrol complex, which provides antioxidant and anti-aging benefits while offering a refreshing feel, regulating sebum secretion, and minimizing pores; For sensitive skin, transdermal peptide-modified liposomes encapsulate centella asiatica-dipotassium glycyrrhizate complex, achieving gentle transdermal penetration through transdermal peptides, avoiding the irritation of traditional penetration enhancers, while the active ingredients soothe inflammation, repair the skin barrier, and achieve gentle anti-aging. The three active ingredients and dual carrier system work synergistically to achieve full skin type compatibility: "moisturizing enough without tightness for dry skin, refreshing without clogging acne for oily skin, and gentle and non-irritating for sensitive skin." This completely solves the core pain point of traditional fixed formulas that cannot cater to multiple skin types, and greatly expands the target audience for the product.

[0022] 2. This invention, tailored to the physicochemical properties of different active ingredients and the characteristics of different skin types, constructs a dual-target delivery system of "transdermal peptide-modified liposomes + natural NLC," overcoming the core defects of traditional carriers. Transdermal peptide-modified liposomes, through the specific mediation of transdermal peptides, can gently and efficiently penetrate the stratum corneum, precisely targeting dermal fibroblasts to achieve precise enrichment of ginsenoside Rb1 and the asiaticoside-dipotassium glycyrrhizate complex in the dermis, avoiding the problems of non-targeting and low transdermal permeability of traditional liposomes. Natural NLC, using all-natural lipids as raw materials, can efficiently penetrate the dense stratum corneum of oily skin, increasing the transdermal permeability of the green tea extract-resveratrol complex. Simultaneously, it can be completely metabolized by the skin without any carrier residue, avoiding skin irritation and barrier damage caused by the residue of traditional synthetic carriers. Experimental verification shows that the dual-target delivery system of this invention can increase the 24-hour cumulative transdermal permeability of active ingredients to over 75% and the dermal enrichment rate to over 50%, significantly improving the efficacy and utilization rate of anti-aging active ingredients.

[0023] 3. The three active ingredients of this invention target three core skin aging targets: insufficient collagen synthesis, oxidative stress damage, and inflammation-mediated premature aging, achieving multi-target synergistic anti-aging: Ginsenoside Rb1 can significantly promote the synthesis of type I and type III collagen and inhibit the expression of matrix metalloproteinases MMP-1 and MMP-3, thereby reducing collagen degradation at its source and improving skin laxity and wrinkles; Green tea extract and resveratrol work together to scavenge free radicals, inhibit lipid peroxidation, block oxidative stress-mediated collagen degradation, and regulate sebum secretion, improving the problem of enlarged pores in oily skin; Centella asiatica extract and dipotassium glycyrrhizate work together to soothe inflammation, inhibit the release of inflammatory factors, block premature skin aging mediated by chronic inflammation, and repair the skin barrier and improve skin tolerance. The three active ingredients are precisely released at their respective skin targets through a differentiated targeted delivery system, and work synergistically. Experimental verification shows that the composition of this invention can promote the synthesis of type I collagen by more than 105%, scaveng the free radicals of DPPH by more than 95%, and improve the depth of wrinkles by more than 40% after 28 days of human trial. It also has multiple effects such as moisturizing, oil control, soothing and repairing.

[0024] 3. The carrier system of this invention uses all natural phospholipids and natural plant oils as raw materials, without synthetic lipids, alcohol, or traditional irritating penetration enhancers. The transdermal peptides are short peptides with excellent biocompatibility, enabling gentle transdermal penetration and avoiding skin irritation caused by traditional penetration enhancers. All active ingredients are of natural plant origin, without irritating chemical components, and are friendly to sensitive skin. Safety tests have verified that the composition of this invention is non-irritating to the skin, showed no adverse reactions in human patch tests, had a carrier residue rate of almost 0, and will not damage the skin barrier with long-term use. It is suitable for all skin types, including sensitive skin, and its safety is far superior to existing technology products. Detailed Implementation

[0025] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0026] In the following examples and comparative examples, the cosmetically acceptable excipients used a uniform basic formulation, specifically in parts by weight: 6 parts glycerin, 4 parts propylene glycol, 0.2 parts sodium hyaluronate, 2 parts jojoba oil, 1 part squalane, 1.2 parts glyceryl stearate citrate, 0.8 parts cetearyl alcohol, 0.2 parts carbomer, 0.1 parts xanthan gum, 0.5 parts phenoxyethanol, and deionized water to a total of 100 parts. All examples and comparative examples only adjusted the composition and content of the active functional component and the targeted delivery carrier system; the remaining excipient components and contents remained completely consistent to ensure single-variable control in the experiments.

[0027] Example 1 This embodiment provides an anti-wrinkle and firming composition suitable for various skin types. The components are as follows, by weight: Active functional components: ginsenoside Rb1 2.0 parts, green tea extract-resveratrol complex (green tea extract:resveratrol=2:1) ​​1.5 parts, asiaticoside-dipotassium glycyrrhizate complex (asiaticoside:dipotassium glycyrrhizate=1:1) 1.0 parts, totaling 4.5 parts; Targeted delivery carrier system: 3.0 parts of transdermal peptide-modified liposomes (TD-1 peptide modified), 2.0 parts of natural NLC, totaling 5.0 parts; Additives: The above-mentioned unified basic formula, plus deionized water to make up to 100 parts.

[0028] The preparation method is as follows: Preparation of the S1 drug delivery system: (1) Preparation of transdermal peptide-modified liposomes carrying ginsenoside Rb1: 8g of hydrogenated lecithin, 2g of cholesterol, 1g of polyethylene glycol succinate, and 20g of ginsenoside Rb1 were weighed and added to 500mL of anhydrous ethanol. The mixture was stirred in a water bath at 35℃ until completely dissolved to obtain an organic phase. The organic phase was transferred to a rotary evaporator and the anhydrous ethanol was removed by rotary evaporation under reduced pressure at 40℃ and -0.09MPa, forming a uniform and transparent lipid film on the inner wall of the flask. The liposomes were then filled with... Add PBS buffer (pH 7.4) to 100g, hydrate in a 40℃ water bath for 30min to obtain a liposome suspension; place the liposome suspension in a probe-type sonicator and sonicate in an ice bath for 10min (power 200W, sonication for 3s, interval 5s) to obtain the initial liposomes; weigh 1g of TD-1 transdermal peptide and add it to the initial liposomes, incubate at 37℃ for 2h to complete the modification, and obtain transdermal peptide-modified liposomes loaded with ginsenoside Rb1. Take 1.8 portions for later use. (2) Preparation of transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex: Weigh 7g of hydrogenated lecithin, 2g of cholesterol, 1g of polyethylene glycol succinate, 5g of asiaticoside, and 5g of dipotassium glycyrrhizate, add them to 500mL of anhydrous ethanol, and prepare them in the same way as above to obtain transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex. Take 1.2 parts for later use; the total of the above two types of liposomes is 3.0 parts, which is the transdermal peptide-modified liposome of this embodiment; (3) Preparation of natural NLC loaded with green tea extract-resveratrol complex: Weigh 6g of shea butter, 3g of cocoa butter, 3g of jojoba oil, 10g of green tea extract, 5g of resveratrol, and 2g of Tween 80, and add deionized water to 100g; mix solid lipids, liquid lipids, green tea extract, and resveratrol, heat in an 80℃ water bath until completely melted, and stir evenly to obtain the oil phase; add Tween 80 to deionized water, heat in an 80℃ water bath until completely dissolved to obtain the aqueous phase; under stirring conditions of 80℃ and 3000rpm, slowly add the oil phase dropwise to the aqueous phase, and continue stirring for 30min after the addition is complete to obtain the colostrum; place the colostrum in a probe-type ultrasonic instrument, sonicate under ice bath conditions for 8min (power 200W, sonication for 3s, interval 5s), and quickly place it in an ice water bath to cool to room temperature to obtain the natural NLC loaded with green tea extract-resveratrol complex, and take 2.0 portions for later use; S2 aqueous phase preparation: Add 6 parts glycerol, 4 parts propylene glycol, 0.2 parts sodium hyaluronate, 0.2 parts carbomer, 0.1 parts xanthan gum, and deionized water to an aqueous phase pot, heat to 80°C, stir at 300 rpm until completely dissolved, homogenize at 3000 rpm for 2 min to obtain the aqueous phase, and keep it at 80°C for later use. S3 oil phase preparation: Add 2 parts of jojoba oil, 1 part of squalane, 1.2 parts of glyceryl stearate citrate, and 0.8 parts of cetearyl alcohol to an oil phase pot, heat to 80°C, and stir at 300 rpm until completely dissolved and homogeneous to obtain the oil phase. Keep it at 80°C for later use. S4 Emulsification and Mixing: Under homogenization conditions of 80℃ and 3000rpm, the oil phase is slowly added to the aqueous phase and homogenized for 5 minutes to obtain an emulsion system; stirring and cooling are started, and when the temperature drops to 45℃, 3.0 parts of transdermal peptide-modified liposomes prepared in step S1 and 2.0 parts of natural NLC are added and stirred evenly at 300rpm; the temperature is further reduced to 40℃, and 0.5 parts of phenoxyethanol are added and stirred evenly; the temperature is further reduced to 25℃, and the material is discharged to obtain the anti-wrinkle and firming composition suitable for multiple skin types in this embodiment.

[0029] Example 2 (Preferred Example) This embodiment provides an anti-wrinkle and firming composition suitable for various skin types. The components are as follows, by weight: Active functional components: ginsenoside Rb1 3.0 parts, green tea extract-resveratrol complex (2:1) 2.0 parts, asiaticoside-dipotassium glycyrrhizate complex (1:1) 2.0 parts, total 7.0 parts; Targeted delivery carrier system: 4.0 parts of transdermal peptide-modified liposomes (TD-1 peptide modified), 3.0 parts of natural NLC, totaling 7.0 parts; Additives: The above-mentioned unified basic formula, plus deionized water to make up to 100 parts.

[0030] The preparation method is as follows: Preparation of the S1 drug delivery system: (1) Preparation of transdermal peptide-modified liposomes loaded with ginsenoside Rb1: The formula is 30g of ginsenoside Rb1, and the rest is the same as in Example 1. Take 2.5 portions of the prepared liposomes for later use. (2) Preparation of transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex: The formula is 10g asiaticoside and 10g dipotassium glycyrrhizate, and the rest is the same as in Example 1. 1.5 parts of the prepared liposomes are used for later use; a total of 4.0 parts of the above two types of liposomes are the transdermal peptide-modified liposomes of this example. (3) Preparation of natural NLC loaded with green tea extract-resveratrol complex: The formula is 13.3g of green tea extract and 6.7g of resveratrol, and the rest is the same as in Example 1. Take 3.0 parts of the natural NLC prepared for later use. Steps S2-S4 are the same as in Example 1, except that the amount of ingredients is adjusted to correspond to the formula of this example, and finally the anti-wrinkle and firming composition suitable for multiple skin types in this example is obtained.

[0031] Example 3 This embodiment provides an anti-wrinkle and firming composition suitable for various skin types. The components are as follows, by weight: Active functional components: ginsenoside Rb1 4.0 parts, green tea extract-resveratrol complex (2:1) 3.0 parts, asiaticoside-dipotassium glycyrrhizate complex (1:1) 2.5 parts, totaling 9.5 parts; Targeted delivery carrier system: 6.0 parts of transdermal peptide-modified liposomes (TD-1 peptide modified), 4.0 parts of natural NLC, totaling 10.0 parts; Additives: The above-mentioned unified basic formula, plus deionized water to make up to 100 parts.

[0032] The preparation method is as follows: Preparation of the S1 drug delivery system: (1) Preparation of transdermal peptide-modified liposomes carrying ginsenoside Rb1: The formula is 40g of ginsenoside Rb1, and the rest is the same as in Example 1. Take 3.5 portions of the prepared liposomes for later use. (2) Preparation of transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex: The formula is 12.5g asiaticoside and 12.5g dipotassium glycyrrhizate, and the rest is the same as in Example 1. 2.5 parts of the prepared liposomes are used for later use; a total of 6.0 parts of the above two types of liposomes are the transdermal peptide-modified liposomes of this example. (3) Preparation of natural NLC loaded with green tea extract-resveratrol complex: The formula is 20g green tea extract and 10g resveratrol, and the rest is the same as in Example 1. Take 4.0 parts of the natural NLC prepared for later use. Steps S2-S4 are the same as in Example 1, except that the amount of ingredients is adjusted to correspond to the formula of this example, and finally the anti-wrinkle and firming composition suitable for multiple skin types in this example is obtained.

[0033] Example 4 This embodiment provides an anti-wrinkle and firming composition suitable for multiple skin types. By weight, the components are the same as in Example 2, except that the transdermal peptide is replaced with TAT peptide, the solid lipids of natural NLC are replaced with beeswax, and the liquid lipids are replaced with grape seed oil.

[0034] The preparation method is the same as in Example 2, except that the corresponding raw materials are adjusted to finally obtain the anti-wrinkle and firming composition suitable for multiple skin types in this example.

[0035] Comparative Example 1 It contains only the excipients of the above-mentioned unified basic formula, without any active functional components or targeted delivery carrier system, and deionized water is added to make up to 100 parts.

[0036] Comparative Example 2 It contains only 3.0 parts of ginsenoside Rb1, with no other active functional components; the carrier is 4.0 parts of ordinary liposomes without transdermal peptide modification, without natural NLC; the other excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0037] Comparative Example 3 It contains only 2.0 parts of green tea extract-resveratrol complex, without other active functional components; the carrier is 3.0 parts of conventional NLC prepared by synthetic lipids, without transdermal peptide modified liposomes; the remaining excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0038] Comparative Example 4 It contains only 2.0 parts of asiaticoside-dipotassium glycyrrhizate complex, without other active functional components; the carrier is 4.0 parts of ordinary liposomes without transdermal peptide modification, without natural NLC; the other excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0039] Comparative Example 5 It contains 7.0 parts of all the active functional components of Example 2, without any targeted delivery carrier system, and the active ingredients are added directly; the other excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0040] Comparative Example 6 It contains 7.0 parts of all active functional components from Example 2; all active components are encapsulated in 7.0 parts of ordinary liposomes without transdermal peptide modification, and there is no natural NLC; the remaining excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0041] Comparative Example 7 It contains 7.0 parts of all active functional components from Example 2; all active components are encapsulated in 7.0 parts of conventional NLC prepared from synthetic lipids, without transdermal peptide-modified liposomes; the remaining excipients are the same as in Example 2, and deionized water is added to make up to 100 parts.

[0042] Comparative Example 8 Remove the ginsenoside Rb1 component. The remaining active functional components (2.0 parts of green tea extract-resveratrol complex and 2.0 parts of asiaticoside-dipotassium glycyrrhizate complex), carrier system, and excipients are the same as in Example 2. Deionized water is added to make up to 100 parts.

[0043] Comparative Example 9 The green tea extract-resveratrol complex component was removed, while the remaining active functional components (ginsenoside Rb1 3.0 parts, asiaticoside-dipotassium glycyrrhizate complex 2.0 parts), carrier system, and excipients were the same as in Example 2, and deionized water was added to make up to 100 parts.

[0044] Comparative Example 10 Remove the asiaticoside-dipotassium glycyrrhizate complex component. The remaining active functional components (ginsenoside Rb1 3.0 parts, green tea extract-resveratrol complex 2.0 parts), carrier system, and excipients are the same as in Example 2. Deionized water is added to make up to 100 parts.

[0045] Comparative Example 11 The transdermal peptide-modified liposomes in Example 2 were replaced with an equal mass of ordinary liposomes without transdermal peptide modification. The remaining active components, natural NLCs, and excipients were the same as in Example 2. Deionized water was added to bring the total to 100 parts.

[0046] Comparative Example 12 The natural NLC in Example 2 was replaced with conventional NLC prepared from synthetic lipids of equal mass. The remaining active components, transdermal peptide-modified liposomes, and excipients were the same as in Example 2. Deionized water was added to make up to 100 parts.

[0047] Performance testing 1. In vitro transdermal performance testing The Franz vertical diffusion cell method was used, with a receiving cell volume of 7 mL and an effective diffusion area of ​​1.766 cm². Fresh detached pig skin was taken, subcutaneous fat was removed, and the skin was rinsed with physiological saline. It was then fixed between diffusion cells, with the stratum corneum facing the supply cell and the dermis facing the receiving cell. 7 mL of pH 7.4 PBS solution was added to the receiving cell, and the mixture was incubated at 32°C with stirring at 300 rpm. 1 g of the compositions from each example and comparative example was added to the supply cell. The receiving solution was collected after 24 hours, and the content of active ingredients was detected by HPLC to calculate the cumulative transdermal penetration rate over 24 hours. After the experiment, the dermis was peeled off, and the content of active ingredients was extracted and detected to calculate the dermal enrichment rate. The residual amount of carrier in the skin was detected by gas chromatography, and the carrier residue rate was calculated.

[0048] 2. In vitro anti-aging efficacy testing (1) Type I collagen synthesis promotion rate: Human skin fibroblasts were seeded in 96-well plates and cultured until the confluence reached 80%. A 100-fold diluted sample was added, and a blank control group was set up. After 48 hours of culture, the content of type I collagen in the supernatant was detected by ELISA kit, and the collagen synthesis promotion rate was calculated.

[0049] (2) MMP-1 inhibition rate: The same cell culture method as above was used. The MMP-1 content in the supernatant was detected by ELISA kit and the MMP-1 inhibition rate was calculated.

[0050] (3) DPPH free radical scavenging rate: The sample was diluted 10 times with anhydrous ethanol and mixed with an equal volume of 0.2 mmol / L DPPH solution. The sample was then allowed to stand in the dark for 30 min, and the absorbance was measured at 517 nm to calculate the DPPH free radical scavenging rate.

[0051] 3. Human trial efficacy and skin type compatibility testing 120 healthy volunteers aged 35-55 were recruited and divided into three groups: dry skin (stratum corneum moisture content <10%), oily skin (sebum secretion rate >80μg / cm²·h), and sensitive skin (lactic acid stinging test score ≥3 points), with 40 volunteers in each group. These were randomly divided into 16 subgroups, corresponding to 4 practical examples and 12 comparative examples, with 2-3 volunteers in each subgroup. Volunteers used the corresponding samples morning and evening after cleansing for 28 consecutive days, without using other functional skincare products during the trial. Before and after 28 days of use, wrinkle depth, skin elasticity, stratum corneum moisture content, sebum secretion rate, and erythema index were measured using a professional skin testing instrument, and the corresponding improvement / enhancement rates were calculated. After the trial, the incidence of adverse reactions and satisfaction levels were recorded.

[0052] 4. Safety testing (1) Rabbit ear skin irritation test: Healthy New Zealand white rabbits were selected and administered the drug once or multiple times (once a day for 7 consecutive days). The erythema and edema of the rabbit ear skin were observed. The irritation score was calculated according to the standard. 0 points was no irritation, 0.5-2 points was mild irritation, 2-6 points was moderate irritation, and >6 points was severe irritation.

[0053] (2) Human skin patch test: 30 healthy volunteers were selected. The sample was added to the patch tester and applied to the flexor side of the forearm. The patch was removed after 24 hours. Skin reactions were observed at 30 minutes, 24 hours and 48 hours. The incidence of adverse reactions was statistically analyzed.

[0054] Table 1. 24-hour transdermal performance test data (average value) for each example and comparative example.

[0055] Table 2. In vitro anti-aging efficacy test data (average values) for each embodiment and comparative example.

[0056] Table 3. Efficacy test data (average) for dry skin group after 28 days of human trial.

[0057] Table 4. Efficacy test data (average) for oily skin group after 28 days of human trial.

[0058] Table 5. Efficacy test data (average) for sensitive skin group after 28 days of human trial.

[0059] Table 6 Safety test data for each embodiment and comparative example.

[0060] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An anti-wrinkle and firming composition suitable for multiple skin types, characterized in that, The product comprises, by weight, the following components: 2.5-9.5 parts of active functional component, 5-10 parts of targeted delivery carrier system, and 80.5-92.5 parts of cosmetically acceptable excipients; wherein the active functional component includes 2.0-4.0 parts of ginsenoside Rb1, 1.5-3.0 parts of green tea extract-resveratrol complex, and 1.0-2.5 parts of asiaticoside-dipotassium glycyrrhizate complex; and the targeted delivery carrier system includes 3.0-6.0 parts of transdermal peptide-modified liposomes and 2.0-4.0 parts of natural nanostructured lipid carriers.

2. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, In the green tea extract-resveratrol complex, the mass ratio of green tea extract to resveratrol is (1-3):

1.

3. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, In the asiaticoside-dipotassium glycyrrhizate complex, the mass ratio of asiaticoside to dipotassium glycyrrhizate is (1-2):

1.

4. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, In the transdermal peptide-modified liposomes, the transdermal peptide used for modification is at least one of TAT peptide, RDP peptide, and TD-1 peptide.

5. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, The lipid membrane material of the transdermal peptide-modified liposome is at least one of natural phospholipids, cholesterol, and polyethylene glycol succinate; the natural phospholipid is at least one of soybean lecithin, egg yolk lecithin, and hydrogenated lecithin.

6. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, The lipid material of the natural nanostructured lipid carrier is a mixture of solid lipids and liquid lipids. The solid lipid is at least one of shea butter, cocoa butter, and beeswax, and the liquid lipid is at least one of jojoba oil, sweet almond oil, and grape seed oil. The mass ratio of solid lipids to liquid lipids is (2-4):

1.

7. The anti-wrinkle and firming composition suitable for multiple skin types according to claim 1, characterized in that, The cosmetic excipients acceptable to the product include 5-20 parts of moisturizer, 2-10 parts of emollient, 1-5 parts of emulsifier, 0.1-2 parts of thickener, 0.05-1 part of preservative, and deionized water to make up to 100 parts.

8. A method for preparing an anti-wrinkle and firming composition suitable for multiple skin types as described in any one of claims 1-7, characterized in that, Includes the following steps: Preparation of S1 drug delivery system: transdermal peptide-modified liposomes loaded with ginsenoside Rb1, natural nanostructured lipid carriers loaded with green tea extract-resveratrol complex, and transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex were prepared respectively. S2 aqueous phase preparation: Mix the humectant, thickener and deionized water, heat and stir until completely dissolved, homogenize to obtain the aqueous phase; S3 oil phase preparation: Mix the emollient and emulsifier, heat and stir until completely homogeneous to obtain the oil phase; S4 Emulsification and Mixing: Add the oil phase to the aqueous phase, homogenize and emulsify, cool down, add the three drug delivery systems prepared in step S1, stir evenly, add preservatives, stir evenly, cool down to room temperature, and the product is obtained.

9. The preparation method according to claim 8, characterized in that, In step S1, the preparation method of the transdermal peptide-modified liposomes loaded with ginsenoside Rb1 is as follows: the lipid membrane material and ginsenoside Rb1 are dissolved in an organic solvent, the organic solvent is removed by rotary evaporation under reduced pressure to form a uniform lipid film, hydration is carried out by adding an hydration solution, and ultrasonic treatment is performed to obtain the initial liposomes; the transdermal peptides are mixed with the initial liposomes and incubated for modification to obtain the transdermal peptide-modified liposomes loaded with ginsenoside Rb1; the preparation method of the transdermal peptide-modified liposomes loaded with asiaticoside-dipotassium glycyrrhizate complex is the same as that of the transdermal peptide-modified liposomes loaded with ginsenoside Rb1.

10. The use of a multi-skin-type-compatible anti-wrinkle and firming composition as described in any one of claims 1-7 in the preparation of anti-wrinkle, firming, soothing, and repairing skincare products.

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