Distributing and mixing device for phosphatase inhibitor

Abstract

The utility model discloses a distributing and mixing device for phosphatase inhibitors, which relates to the technical field of distributing and mixing devices for inhibitors and comprises a magnetic stirrer, a mixing bottle is mounted on the magnetic stirrer, supports are arranged on two sides of the magnetic stirrer, and a proportioning component is arranged between the two supports. Proportioning the phosphatase inhibitor with a solvent when the phosphatase inhibitor is diluted; a stirring assembly is arranged at the bottom end of the proportioning assembly and is used for diluting and stirring the phosphatase inhibitor and the solvent. According to the invention, centralized treatment of distribution and mixing of the phosphatase inhibitor is realized, the step that an operator needs to quantitatively transfer a reagent by using a pipette for many times is reduced, and the operation steps of an experimenter are simplified. The problem that an operator needs to continuously and accurately sample, stir and dropwise add the phosphatase inhibitor in the distribution and mixing process is solved, the operation difficulty and steps of the operator are simplified, and operation is more concentrated and convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of inhibitor distribution and mixing devices, in particular to a phosphatase inhibitor distribution and mixing device. Background Art

[0002] Phosphatase inhibitors are a class of compounds that specifically or nonspecifically inhibit the activity of phosphatases, thereby preventing them from removing phosphorylated modifications from proteins or other molecules. Phosphorylation is an important post-translational modification of proteins, involved in regulating various cellular signaling pathways, metabolic processes, and cell cycle control. Phosphatase inhibitors can affect protein phosphorylation and are therefore used in the treatment of various biological samples to protect the integrity of phosphorylated proteins.

[0003] To dispense and mix the phosphatase inhibitor, first remove the phosphatase inhibitor from frozen storage and allow it to warm to room temperature. Then, dilute the phosphatase inhibitor according to your calculations. Finally, add an appropriate amount of the diluent to the pre-chilled biological sample and stir to mix.

[0004] When distributing and mixing reagents for phosphatase inhibitors, a pipette is required to quantitatively transfer the reagents into a container at each step. In order to achieve better dilution and mixing effects, the reagents need to be added dropwise while stirring. This requires high operator skills and the operation steps are cumbersome, time-consuming, and labor-intensive. Utility Model Content

[0005] The main purpose of the utility model is to provide a phosphatase inhibitor distribution and mixing device, which can effectively solve the technical problems in the background technology.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A phosphatase inhibitor dispensing and mixing device comprises a magnetic stirrer, a mixing bottle mounted on the magnetic stirrer, brackets disposed on both sides of the magnetic stirrer, and a proportioning assembly disposed between the two brackets for proportioning the phosphatase inhibitor with a solvent during dilution.

[0008] The bottom end of the proportioning component is provided with a stirring component for diluting and stirring the phosphatase inhibitor and the solvent.

[0009] As a further solution of the present invention, the proportioning component includes a solvent bottle and a reagent bottle, and the bottom ends of the solvent bottle and the reagent bottle are fixedly installed with a one-way valve, a sealing plug 1 is slidably installed in the solvent bottle, and a sealing plug 2 is slidably installed in the reagent bottle.

[0010] As a further scheme of the utility model, the upper and lower ends of the sealing plug one and the sealing plug two are provided with air holes, the top end of the air hole is detachably provided with a sealing cover, the surface of the sealing plug one is fixedly provided with a connecting rod at the top end, the connecting rod is slidably connected with the sealing plug two, and the connecting rod one and the sealing plug two are fixed by bolts.

[0011] As a further scheme of the utility model, the stirring assembly comprises a proportioning tank, the top end of the proportioning tank is connected with the bottom end of the one-way valve, the inside of the proportioning tank is provided with a power compartment at the bottom end, the power compartment is fixedly provided with a motor, the top end of the motor is fixedly provided with a magnet, and the inside of the proportioning tank is provided with a magnetic sub.

[0012] As a further scheme of the utility model, the top end of the proportioning tank is fixedly provided with a one-way air valve, and the bottom end of the proportioning tank is fixedly provided with a valve.

[0013] As a further scheme of the utility model, the inside of the support is rotatably provided with screw rods, the screw rods are fixedly provided with a fixing frame between the screw rods, and the fixing frame is fixedly connected with the solvent bottle, the reagent bottle and the proportioning tank.

[0014] The utility model discloses the beneficial effects are as follows:

[0015] Through setting up the magnetic stirrer, the proportioning assembly, the stirring assembly and the mixing bottle, the distribution and mixing of phosphatase inhibitor are concentrated, and the reagent is not needed to be transferred by using the pipette for many times, so that the operation steps of the distribution and mixing of phosphatase inhibitor are simplified.

[0016] Through setting up the proportioning assembly and the stirring assembly, only the phosphatase inhibitor needs to be accurately sampled, the connecting point of the sealing plug two and the connecting rod is adjusted, the phosphatase inhibitor can be diluted in different proportions, and the phosphatase inhibitor is stirred by the magnetic sub during dilution, and the proportioning of the phosphatase inhibitor is more convenient and uniform. DRAWINGS

[0017] Figure 1 It is whole structure schematic view of the utility model discloses a kind of distribution and mixing device of phosphatase inhibitor;

[0018] Figure 2 It is whole structure schematic view of the proportioning assembly of the utility model discloses a kind of distribution and mixing device of phosphatase inhibitor;

[0019] Figure 3 It is sectional structure schematic view of the sealing plug one and the sealing plug two of the utility model discloses a kind of distribution and mixing device of phosphatase inhibitor;

[0020] Figure 4 It is structure schematic view of the stirring assembly of the utility model discloses a kind of distribution and mixing device of phosphatase inhibitor.

[0021] Figure 5 The utility model is a schematic diagram of the internal structure of a proportioning tank of a phosphatase inhibitor distribution and mixing device.

[0022] In the figure: 1. Magnetic stirrer; 2. Bracket; 3. Fixing frame; 4. Screw; 5. Proportioning assembly; 6. Stirring assembly; 7. Mixing bottle; 51. Solvent bottle; 52. Reagent bottle; 53. Sealing plug 1; 54. Sealing plug 2; 55. Connecting rod; 56. Bolt; 57. One-way valve; 58. Vent; 59. Sealing cover; 61. Proportioning tank; 62. One-way air valve; 63. Power compartment; 64. Motor; 65. Magnet; 66. Magnetic element; 67. Valve. DETAILED DESCRIPTION

[0023] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0024] like Figure 1-5 As shown, a phosphatase inhibitor dispensing and mixing device includes a magnetic stirrer 1, a mixing bottle 7 is mounted on the magnetic stirrer 1, brackets 2 are provided on both sides of the magnetic stirrer 1, screws 4 are rotatably mounted in the brackets 2, and fixing frames 3 are threadedly mounted on the two screws 4. The fixing frames 3 are provided with a proportioning component 5 and a stirring component 6, which are used to proportion the volume of the phosphatase inhibitor and the solvent, thereby performing a constant volume dilution of the phosphatase inhibitor. The stirring component 6 is used to stir and mix the phosphatase inhibitor and the solvent uniformly. The rotation of the screw 4 can drive the fixing frame 3 to move, thereby adjusting the height of the proportioning component 5 and the stirring component 6 to accommodate different mixing bottles 7.

[0025] In the present embodiment, proportioning assembly 5 comprises solvent bottle 51 and reagent bottle 52, and solvent bottle 51 is used for the placement of diluting solvent, and reagent bottle 52 is used for the placement of phosphatase inhibitor, and reagent bottle 52 is transparent material, and internal reagent can be observed.The bottom of solvent bottle 51 and reagent bottle 52 is all fixedly installed with one-way valve 57, and sealing plug 2 54 and sealing plug one 53 are slidably installed respectively in solvent bottle 51 and reagent bottle 52, and sealing plug 2 54 and sealing plug one 53 are all provided with scale (not shown), for regulating relative position between the two more accurately.Connecting rod 55 is fixedly installed on sealing plug one 53, sliding connection between connecting rod 55 and sealing plug 2 54, and is fixed by bolt 56, thereby can regulate the position of sealing plug 2 54, thereby make sealing plug one 53 and sealing plug 2 54 different in the motion stroke of reagent bottle 52 and solvent bottle 51.

[0026] Preferably, the diameter of the solvent bottle 51 and the reagent bottle 52 is 10 times different, the sealing plug one 53 and the sealing plug two 54 travel the same height in the reagent bottle 52 and the solvent bottle 51, the volume of the two is 100 times different, the dilution ratio between the phosphatase inhibitor and the solvent can be adjusted by adjusting the travel height of the sealing plug two 54 in the solvent bottle 51.

[0027] In the embodiment, the upper and lower ends of the sealing plug one 53 and the sealing plug two 54 are provided with the air vent 58, which is used for the communication between the solvent bottle 51, the reagent bottle 52 and the outside after the mixing is completed, so that the sealing effect of the sealing plug one 53 and the sealing plug two 54 is released, and the sealing plug one 53 and the sealing plug two 54 are convenient to take out. The top end of the air vent 58 is detachably provided with the sealing cover 59, which is preferably screwed or buckled, and is used for sealing the air vent 58 without affecting the work of the sealing plug one 53 and the sealing plug two 54.

[0028] In the embodiment, the bottom end of the one-way valve 57 is fixedly connected with the mixing tank 61, which is used for the phosphatase inhibitor and the solvent to enter the mixing tank 61 through mixing, the top end of the mixing tank 61 is fixedly provided with the one-way air valve 62, the communication direction of the one-way air valve 62 is from the mixing tank 61 to the outside air, so that the phosphatase inhibitor and the solvent can smoothly enter the mixing tank 61, and the dust and impurities in the outside cannot enter the inside of the mixing tank 61, so that the whole mixing process is more reliable. The inside of the mixing tank 61 is provided with the power compartment 63, the inside of the power compartment 63 is fixedly provided with the motor 64, the motor 64 is fixedly connected with the magnet 65, the mixing tank 61 is provided with the magnetic sub 66, the motor 64 drives the magnet 65 to rotate through the magnetic force, so as to stir and mix the phosphatase inhibitor and the solvent in the mixing tank 61, thereby diluting the phosphatase inhibitor.

[0029] In the embodiment, the bottom end of the mixing tank 61 is provided with the valve 67, and the valve 67 is located at the upper end of the mixing bottle 7. By opening the valve 67, the uniformly mixed phosphatase inhibitor diluent in the mixing tank 61 can be added to the mixing bottle 7, so as to mix the phosphatase inhibitor with the sample. During the mixing, the sample can be stirred by the magnetic stirrer 1 to ensure uniform mixing.

[0030] It should be noted that the utility model is a kind of phosphatase inhibitor dispensing and mixing device, when using, by rotating screw rod 4, screw rod 4 drives fixed frame 3 to move, so as to adjust the distance between valve 67 and magnetic stirrer 1, so as to adapt to different mixing bottles 7.

[0031] After calculation, the thawed phosphatase inhibitor is taken out by pipetting and placed in the reagent bottle 52, and then an excess amount of dilution solvent is added to the solvent bottle 51. The height of the sealing plug 54 is adjusted according to the dilution ratio of the phosphatase inhibitor and the solvent and according to the scale of the phosphatase inhibitor in the reagent bottle 52, and then the sealing plug 54 and the solvent bottle 51 are fixed by bolts 56. The sealing cover 59 is opened, and the sealing plug 1 53 is moved so that the bottom end of the sealing plug 1 53 is flush with the liquid surface, and then the sealing cover 59 is closed. Then, by pressing the connecting rod 55, the sealing plug 1 53 and the sealing plug 2 54 are driven to move synchronously. However, the sealing plug 1 53 moves directly along the reagent bottle 52, and the sealing plug 2 54 will move different distances in the air according to different ratios before moving along the solvent bottle 51, squeezing the solvent, so that the phosphatase inhibitor and the solvent enter the proportioning tank 61 through the one-way valve 57 according to the fixed ratio.

[0032] The motor 64 is started, and the motor 64 drives the magnet 65 to rotate. The magnet 65 drives the magnet 66 to rotate through the action of magnetic force, thereby mixing the phosphatase inhibitor and the solvent entering the proportioning tank 61. The stirring is performed by magnetic force, which can effectively reduce the influence of shear force.

[0033] After the distribution is completed, the valve 67 is opened to allow the reagent in the proportioning tank 61 to slowly flow out into the mixing bottle 7 and be added to the sample, and the sample is stirred and mixed by the magnetic stirrer 1.

[0034] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A device for dispensing and mixing a phosphatase inhibitor, comprising a magnetic stirrer (1), characterized in that: A mixing bottle (7) is installed on the magnetic stirrer (1), and brackets (2) are provided on both sides of the magnetic stirrer (1). A proportioning component (5) is provided between the two brackets (2) for proportioning the phosphatase inhibitor with the solvent when diluting the phosphatase inhibitor; The bottom end of the proportioning component (5) is provided with a stirring component (6) for diluting and stirring the phosphatase inhibitor and the solvent.

2. A phosphatase inhibitor dispensing and mixing device according to claim 1, characterized in that: The proportioning component (5) includes a solvent bottle (51) and a reagent bottle (52), wherein the bottom ends of the solvent bottle (51) and the reagent bottle (52) are fixedly installed with a one-way valve (57), a sealing plug (53) is slidably installed in the solvent bottle (51), and a sealing plug (54) is slidably installed in the reagent bottle (52).

3. A phosphatase inhibitor dispensing and mixing device according to claim 2, characterized in that: A vent hole (58) is provided through the upper and lower ends of the sealing plug 1 (53) and the sealing plug 2 (54), and a sealing cover (59) is detachably installed at the top end of the vent hole (58). A connecting rod (55) is fixedly installed on the surface of the sealing plug 1 (53) near the top end, and the connecting rod (55) is slidably connected to the sealing plug 2 (54). The connecting rod (55) and the sealing plug 2 (54) are fixed by bolts (56).

4. The phosphatase inhibitor dispensing and mixing device according to claim 1, characterized in that: The stirring assembly (6) includes a proportioning tank (61), the top end of the proportioning tank (61) is connected to the bottom end of the one-way valve (57), a power compartment (63) is provided inside the proportioning tank (61) near the bottom end, a motor (64) is fixedly installed in the power compartment (63), a magnet (65) is fixedly installed at the top end of the motor (64), and a magnet (66) is installed inside the proportioning tank (61).

5. A phosphatase inhibitor dispensing and mixing device according to claim 4, characterized in that: A one-way air valve (62) is fixedly mounted on the top end of the proportioning tank (61), and a valve (67) is fixedly mounted on the bottom end of the proportioning tank (61).

6. The phosphatase inhibitor dispensing and mixing device according to claim 1, characterized in that: A screw rod (4) is rotatably mounted in the bracket (2), a fixing frame (3) is threadedly mounted between the two screw rods (4), and the fixing frame (3) is fixedly connected to the solvent bottle (51), the reagent bottle (52) and the proportioning tank (61).

Images