A device for cell resuscitation and cell suspension collection

Abstract

The utility model relates to cell medical field discloses a kind of for cell liquid supplement and cell suspension collection device, including collection bottle, filter screen, sealing ring and injection assembly, the filter screen is slidably connected in the collection bottle top, the handle is fixedly connected in the filter screen inside, the sealing ring is slidably connected in the collection bottle outside, the collection bottle top and the sealing ring inside are all fixedly connected with four magnets, the injection assembly is installed in the sealing ring top, the positioning assembly is installed in the injection assembly outside, the injection assembly includes pipeline, and the pipeline output end is fixedly connected in the sealing ring top.In the utility model, by pulling slide bar, piston compression spring one is driven, simultaneously under the cooperation of two check valves, after supplement liquid, cell suspension is inhaled into shell from pipeline left end, then slide bar is pushed, and it will be transported to collection bottle along pipeline right side, and liquid supplement or cell harvesting can be realized quickly, and it is transported to collection bottle, and the equipment structure is simple, easy to operate, and low in operating cost.

Description

Technical Field

[0001] This utility model relates to the field of cell therapy, and in particular to a device for cell rehydration and cell suspension collection. Background Technology

[0002] Cell replenishment and cell suspension collection devices are common experimental equipment in the biomedical and cell therapy fields, widely used in cell culture, cell therapy, and biopharmaceutical industries. The main function of the cell replenishment device is to provide the necessary nutrients to cultured cells, maintain a stable culture environment, and ensure cell growth, proliferation, and biological activity. During cell culture, the replenishment of liquid is precisely regulated by an automated control system. It can also be used to collect cell suspensions, and is especially suitable for batch and large-scale cell harvesting processes. Using this equipment can greatly reduce manual operation, reduce the risk of exogenous factor contamination, make it more suitable for industrial preparation, improve cell culture processing efficiency, facilitate product quality control, and reduce production costs.

[0003] Cell replenishment and cell suspension collection devices are used to recover cell culture supernatants. They are mainly used for collecting cell culture supernatants to prepare raw materials for exosome production. The device uses centrifugation, filtration or other physicochemical methods to efficiently collect the culture medium, ensuring the harvest quantity, harvest efficiency and quality. Modern automated harvesting devices combine automation technology to efficiently complete the recovery process, reduce manual operation, make it easier to control the quality uniformity, and greatly reduce the risk of microbial introduction.

[0004] The combination of these two technologies plays an important role in the fields of bioengineering and cell medicine, providing efficient, safe and stable technical support for cell culture and harvesting. However, due to the precise and complex structure of such automated harvesting devices, maintenance is difficult and the purchase and use costs are high. Therefore, a device for cell replenishment and cell suspension collection is proposed to solve the above problems. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides a device for cell rehydration and cell suspension collection, which aims to improve the problems of difficult maintenance and high purchase and use costs in such automatic harvesting devices due to their precise and complex structure.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a device for cell rehydration and cell suspension collection, comprising a collection bottle, a filter screen slidably connected to the top of the collection bottle, a handle fixedly connected inside the filter screen, a sealing ring slidably connected to the outside of the collection bottle, four magnets fixedly connected to the top of the collection bottle and inside the sealing ring, an injection component installed on the top of the sealing ring, and a positioning component installed outside the injection component;

[0007] The injection assembly includes a pipe, the output end of which is fixedly connected to the top of the sealing ring. A housing is fixedly connected to the outside of the pipe, and a slide rod is slidably connected inside the housing. Multiple slots are provided on both the left and right sides of the slide rod. A piston is fixedly connected to the rear end of the slots, and a spring is fixedly connected to the front side of the piston. Two one-way valves are fixedly connected to the outside of the pipe, and two valves are fixedly connected to the outside of the pipe.

[0008] As a further description of the above technical solution:

[0009] The positioning component includes two housings. The outer side of the housing is fixedly connected to the front side of the outer shell. A second spring is fixedly connected to the inner wall of the housing. A frame is fixedly connected to the inner side of the second spring. A ball bearing is rotatably connected inside the frame.

[0010] As a further description of the above technical solution:

[0011] The piston is externally slidably connected to the inner wall of the housing, and the four magnets on the upper side and the four magnets on the lower side are magnetically connected.

[0012] As a further description of the above technical solution:

[0013] The front end of the spring is fixedly connected to the inner wall of the outer casing, and the spring is internally sleeved on the outside of the slide rod.

[0014] As a further description of the above technical solution:

[0015] The two check valves are located on the left and right sides of the rear end of the housing, and the valve is located outside the check valve.

[0016] As a further description of the above technical solution:

[0017] The slots are distributed in a straight line at equal intervals.

[0018] As a further description of the above technical solution:

[0019] The outer sleeve is slidably connected to the inner wall of the housing, and the outer ball abuts against the inner wall of the housing.

[0020] As a further description of the above technical solution:

[0021] The ball engages with the outside of the ball and the inside of the slot.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, by pulling the slide rod, the piston is driven to compress the spring. At the same time, with the cooperation of two one-way valves, the liquid to be replenished or the cell suspension is sucked into the outer shell from the left end of the pipe. Then, by pushing the slide rod, it will be transported to the collection bottle along the right side of the pipe. Liquid replenishment or cell harvesting can be quickly realized and transported to the collection bottle. The device has a simple structure, is easy to operate, and has low operating costs.

[0024] 2. In this utility model, the spring pushes the sleeve frame, causing the ball bearing to be locked inside the slot, thereby positioning the slide rod and controlling the volume injected into the collection bottle, thus precisely controlling the liquid flow rate. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of a cell rehydration and cell suspension collection device proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the outer shell of a cell rehydration and cell suspension collection device proposed in this utility model;

[0027] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0028] Figure 4 This is a schematic diagram of the piston structure of a cell rehydration and cell suspension collection device proposed in this utility model;

[0029] Figure 5 This is a schematic diagram of the structure of a ball bearing for a cell rehydration and cell suspension collection device proposed in this utility model.

[0030] Legend:

[0031] 1. Collection bottle; 2. Filter screen; 3. Handle; 4. Sealing ring; 5. Magnet; 6. Pipe; 7. Outer shell; 8. Slide rod; 9. Slot; 10. Piston; 11. Spring 1; 12. Check valve; 13. Valve; 14. Sleeve; 15. Spring 2; 16. Sleeve frame; 17. Ball bearing. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] Reference Figures 1-4This utility model provides an embodiment of a cell rehydration and cell suspension collection device, comprising a collection bottle 1, a filter screen 2 slidably connected to the top of the collection bottle 1, a handle 3 fixedly connected inside the filter screen 2, a sealing ring 4 slidably connected to the outside of the collection bottle 1, and four magnets 5 fixedly connected to the top of the collection bottle 1 and inside the sealing ring 4. The collection bottle 1 is used to store cell rehydration solution and cells, the filter screen 2 is used to filter solid impurities in the cell rehydration solution and cells, wherein the pore size of the filter screen 2 is 70um, allowing single cells to pass through while cell clusters are trapped on the screen, and the filter screen is a disposable sterile screen. The handle 3 is used to facilitate the removal of the filter screen 2 from the collection bottle 1, the sealing ring 4 is used to seal the collection bottle 1, and the magnets 5 are used to fix the sealing ring 4 on the collection bottle 1, wherein the polarity of the sealing ring 4 and the magnets 5 on the collection bottle 1 are opposite, and the sealing ring 4 is fixed by the principle of attraction between opposite shapes. An injection component is installed on the top of the sealing ring 4, and a positioning component is installed outside the injection component.

[0034] The injection assembly includes a pipe 6, the output end of which is fixedly connected to the top of a sealing ring 4. A housing 7 is fixedly connected to the outside of the pipe 6. A sliding rod 8 is slidably connected inside the housing 7. Multiple slots 9 are provided on both sides of the sliding rod 8. A piston 10 is fixedly connected to the rear end of each slot 9. A spring 11 is fixedly connected to the front of the piston 10. Two one-way valves 12 and two valves 13 are fixedly connected to the outside of the pipe 6. The housing 7 connects and protects the internal components. The sliding rod 8 facilitates manual pushing and pulling of the piston 10. The slots 9 engage with a ball bearing 17 to position the sliding rod 8's movement distance. The piston 10 generates positive or negative pressure in the housing 7, thereby drawing in and injecting cell rehydration fluid and cells. The spring 11 pushes the piston 10 back to its original position. The one-way valves... 12 is used to prevent cell rehydration and cell backflow. Valve 13 is used to control the flow rate. The piston 10 is externally slidably connected to the inner wall of the outer shell 7, limiting the movement direction of the piston 10. The four magnets 5 on the upper side and the four magnets 5 on the lower side are magnetically connected to fix the sealing ring 4 to the collection bottle 1. The front end of the spring 11 is fixedly connected to the inner wall of the outer shell 7. The inner part of the spring 11 is sleeved on the outside of the slide rod 8 to keep the spring 11 stable. Two one-way valves 12 are set on the left and right sides of the rear end of the outer shell 7. Valve 13 is set on the outside of the one-way valve 12. The slots 9 are distributed in a straight line at equal intervals to ensure that the cell rehydration and cells move in the specified direction and do not backflow. The materials of the components in this device that come into direct contact with the liquid, such as the pipe 6, collection bottle 1, and sealing ring 4, can be autoclaved.

[0035] Reference Figure 2 , Figure 4 , Figure 5The positioning assembly includes two housings 14. The outer housing 14 is fixedly connected to the front side of the outer casing 7. A second spring 15 is fixedly connected to the inner wall of the housing 14. A frame 16 is fixedly connected to the inner side of the second spring 15. A ball bearing 17 is rotatably connected inside the frame 16. The housing 14 is used to connect and protect the internal parts. The second spring 15 is used to push the frame 16 to move. The frame 16 is used to drive the ball bearing 17 to move synchronously. The ball bearing 17 is used to cooperate with the slot 9 to limit the movement distance of the slide rod 8. The outer side of the frame 16 is slidably connected to the inner wall of the housing 14 to limit the movement distance of the frame 16. The outer side of the ball bearing 17 abuts against the inner wall of the housing 14 to keep the ball bearing 17 stable and prevent it from falling off. The outer side of the ball bearing 17 engages with the inside of the slot 9 to control the movement distance of the slide rod 8.

[0036] Working principle: When cell rehydration solution and cells need to be extracted, first place the filter screen 2 into the collection bottle 1. Then, use the magnet 5 to attach the sealing ring 4 to the top of the collection bottle 1. Next, open the valve 13 and pull the slide rod 8 to compress the piston 10 and spring 11, creating a negative pressure inside the outer shell 7. The cell rehydration solution and cells are then drawn into the outer shell 7 from the left end of the pipe 6. Then, push the slide rod 8 to squeeze the piston 10 and the cell rehydration solution and cells. Under the action of the two one-way valves 12, the cell rehydration solution and cells enter the collection bottle 1 along the right side of the pipe 6 and are filtered by the filter screen 2, which intercepts solid impurities. At the same time, the spring 15 pushes the frame 16. The ball bearing 17 is engaged with the outside of the slot 9, achieving precise control of the liquid flow. Finally, the sealing ring 4 is pulled out from the top of the collection bottle 1, and the filter screen 2 is removed from the inside of the collection bottle 1 for cleaning. This device can realize the functions of liquid replenishment, cell culture supernatant collection, and cell harvesting. It can quantitatively replenish the culture medium, collect exosomes from the culture supernatant, and harvest cells under closed conditions. It can efficiently complete the replenishment and recovery process, reduce manual operation, make it easier to control the quality uniformity, greatly reduce the risk of microbial introduction, improve the efficiency of operation, shorten the process time, improve the utilization rate of equipment and plant, and is more conducive to increasing the annual production capacity of cell products.

[0037] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for cell rehydration and cell suspension collection, comprising a collection bottle (1), characterized in that: The top of the collection bottle (1) is slidably connected to a filter screen (2), and a handle (3) is fixedly connected inside the filter screen (2). A sealing ring (4) is slidably connected to the outside of the collection bottle (1). Four magnets (5) are fixedly connected to the top of the collection bottle (1) and inside the sealing ring (4). An injection component is installed on the top of the sealing ring (4), and a positioning component is installed outside the injection component. The injection assembly includes a pipe (6), the output end of which is fixedly connected to the top of the sealing ring (4). A housing (7) is fixedly connected to the outside of the pipe (6). A slide rod (8) is slidably connected inside the housing (7). Multiple slots (9) are provided on both the left and right sides of the slide rod (8). A piston (10) is fixedly connected to the rear end of the slot (9). A spring (11) is fixedly connected to the front side of the piston (10). Two one-way valves (12) are fixedly connected to the outside of the pipe (6). Two valves (13) are fixedly connected to the outside of the pipe (6).

2. The device for cell rehydration and cell suspension collection according to claim 1, characterized in that: The positioning component includes two housings (14), the outer side of which is fixedly connected to the front side of the outer shell (7), the inner wall of which is fixedly connected to a second spring (15), the inner side of which is fixedly connected to a frame (16), and the inner side of which is rotatably connected to a ball (17).

3. The device for cell rehydration and cell suspension collection according to claim 1, characterized in that: The piston (10) is externally slidably connected to the inner wall of the outer casing (7), and the four magnets (5) on the upper side and the four magnets (5) on the lower side are magnetically connected.

4. The device for cell rehydration and cell suspension collection according to claim 1, characterized in that: The front end of the spring (11) is fixedly connected to the inner wall of the outer shell (7), and the spring (11) is sleeved inside the slide rod (8).

5. The device for cell rehydration and cell suspension collection according to claim 1, characterized in that: Two check valves (12) are disposed on the left and right sides of the rear end of the housing (7), and the valve (13) is disposed outside the check valves (12).

6. The device for cell rehydration and cell suspension collection according to claim 1, characterized in that: The slots (9) are distributed in a straight line at equal intervals.

7. The device for cell rehydration and cell suspension collection according to claim 2, characterized in that: The outer sleeve (16) is slidably connected to the inner wall of the sleeve (14), and the outer ball (17) abuts against the inner wall of the sleeve (14).

8. The device for cell rehydration and cell suspension collection according to claim 2, characterized in that: The ball (17) engages with the outside of the ball and the slot (9) inside the ball.

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