Adipose tissue fluidification device

Abstract

The present invention relates to an adipose tissue fluidification device, specifically an adipose tissue fluidification device for obtaining nanofat for reinjection into a patient as a facial or body filler, comprising a body, two ends aligned along the same geometric axis, and a plurality of channels internal to the body or three meshes internal to the body.

Description

ADIPOSE TISSUE FLUIDIFICATION DEVICE

[0001] The present invention relates to an adipose tissue fluidification device, specifically an adipose tissue fluidification device for obtaining nanofat for reinjection into a patient as a facial or body filler.Background of the Invention

[0002] Fat grafting is a surgical technique used in aesthetic and reconstructive medicine with the aim of reinjecting fat aspirated from a patient in another part of its body.

[0003] Autologous fat transplantation is a safe technique to improve or correct significant contour deformities in a patient's body, being an important technique in reconstructive surgeries. In this procedure, fat is taken from a certain place on the body, such as the abdomen, and reinjected into another region of the patient himself, such as breasts or face.

[0004] Fat grafts and their derivatives have been widely used in tissue engineering due to their volumetric and regenerative potential. Liposuction and adipocyte purification are used for injection into the face as a soft tissue filler, as well as for application to the breasts.

[0005] Currently, fat is collected with suction cannulas with variable diameters, but preferably smaller than 2 mm. This fat is washed with saline solution and, after decantation, the fat present in the supernatant follows the appropriate protocol, such as the Tonnard protocol, in which the fat is mechanically emulsified by 30 passes through a filter with a female Luer Lock connector, with a single-channel with an internal diameter of 2.4 mm, at a conversion speed of 20 mL / seconds between two 20 ml syringes, obtaining microfat, often used to fill deeper facial wrinkles. If it is of interest to obtain nanofat, the emulsification process continues through 1.4 mm, 1.2 mm filters and finally, through a final filter with a single passage, containing micrometric pores, generally between 60 and 500 µm. This method, described by Tonnard, allows the isolation of the stromal vascular fraction (SVF) as well as some cellular components of non-viable adipocytes. The active component, FVE, promotes endothelial proliferation, collagen production, differentiation and proliferation of new cells.

[0006] In this context, patent US2016333305 presents filtering elements for this process consisting of a filtering element for obtaining nanofat, in, and couplers for reducing the size of adipocyte lobules, in Figure 4. Despite being a simple and effective model, the pressure applied to pass fat through single-channel filters can compromise cell viability due to shear force. Therefore, a coupler containing two meshes increases the chance of the device clogging with fibrotic material present in the fat.

[0007] Another protocol for isolation of the FVE involves enzymatic digestion of the extracellular matrix using collagenase. However, this method limits the volume of lipoaspirate to be processed both due to difficulties with the method for large volumes and the high cost of the enzyme. Furthermore, the enzyme has not been approved for clinical use by regulatory agencies, such as the Food and Drug Administration (FDA) of the United States of America.

[0008] Thus, it is necessary to develop an adipose tissue fluidification device, specifically an adipose tissue fluidification device to obtain nanofat for reinjection into a patient as a facial or body filler, which minimizes microbiological contamination, and which improves cell viability and the efficiency of the nanofat obtaining process.Invention Objective

[0009] An objective of the invention is to provide an adipose tissue fluidification device, specifically an adipose tissue fluidification device for obtaining nanofat for reinjection into a patient as a facial or body filler, which minimizes microbiological contamination, improves cell viability and the efficiency of the nanofat obtaining process, and without the need for the use of enzymes for degradation of the extracellular matrix.Summary of the Invention

[0010] According to one embodiment of the present invention, an adipose tissue fluidization device is provided, wherein the adipose tissue fluidization device comprises a body comprising two ends aligned along a same geometric axis, each end comprising an adipose tissue inlet / outlet connection.

[0011] Additionally, in an alternative embodiment of the present invention, the adipose tissue fluidization device is a multi-channel filter.

[0012] In an alternative embodiment of the present invention, the adipose tissue fluidization device is a single-channel filter, and the body of the adipose tissue fluidization device comprises at least three meshes spaced apart, wherein the meshes are of the mesh type and have weaves that may have the same or different sizes.Brief Description of the Drawings

[0013] is a perspective view of a multi-channel adipose tissue fluidization device of the present invention;

[0014] is a front view of internal details of the multi-channel adipose tissue fluidization device of the present invention; and

[0015] is a perspective view of a single-passage adipose tissue filtration and fluidization device of the present invention.Detailed description of the invention

[0016] In the following, the invention will be described in more detail with reference to FIGS. 1 to 3.

[0017] With the intention of offering an adipose tissue fluidification device that enables the processing of fat for fat grafting, in order to maintain tissue viability and cell isolation in a closed, sterile environment, avoiding contamination, an adipose tissue fluidification device is provided comprising elements for fat fluidification with several channels of specific diameter (2.4, 1.4 and 1.2 mm), in which the increase in area due to the presence of the several channels reduces the positive pressure applied and, consequently, increases cell viability.

[0018] Alternatively, an adipose tissue fluidification device for obtaining nanofat can be provided with three meshes spaced apart, more efficiently retaining the fibrotic material, and increasing the quality of the obtained nanofat.

[0019] Furthermore, both the filters and the coupler can be integrated into a single system, facilitating handling by the medical team.

[0020] The embodiments of the present invention do not require the use of the collagenase enzyme, prohibited by the FDA and not indicated by the European Union, to obtain nanofat and, in addition, the developed accessories are portable, which facilitates transportation and handling by the medical team, and can be single-use or reusable.

[0021] According to FIGS. 1 and 2, a multi-channel adipose tissue fluidization device 10 is provided, wherein the adipose tissue fluidization device 10 comprises a body 15 comprising two ends 20A,B aligned along a same axis, wherein each end 20A,B comprises an adipose tissue inlet / outlet connection.

[0022] The adipose tissue fluidization device 10 is a multi-channel filter, further comprising a plurality of channels 25 internal to the body 15 of the adipose tissue fluidization device 10, wherein the plurality of channels 25 have the same diameter, ranging from 1.2 to 2.4 mm, preferably 1.2, 1.4 and 2.4 mm.

[0023] The ends 20A,B of the adipose tissue fluidization device 10 comprise a connection for syringes, preferably a Luer Lock type connection.

[0024] During use of the adipose tissue fluidization device 10, a doctor will couple a syringe containing the liposuctioned tissue to a first end 20A of the adipose tissue fluidization device 10 via a Luer Lock connection and another, new, sterile syringe to a second end 20B of the adipose tissue fluidization device 10, also via a Luer Lock connection. Note that the notation of first and second ends is used only to facilitate the description of the invention, and does not imply a necessary order to be followed.

[0025] After attaching the syringes to the ends 20A,B, the doctor must activate the syringes so that the liposuctioned tissue comes out of one syringe, passes through the channels of the adipose tissue fluidization device 10, and is transferred to the other syringe. This step can be performed as many times as the doctor deems necessary, however, practical tests have indicated that this procedure should be performed at least 11 times, preferably at a speed of 10mL / s.

[0026] The physician may additionally pass the liposuctioned tissue through various adipose tissue fluidization devices 10, for example, initially passing it through an adipose tissue fluidization device 10 with 2.4 mm diameter channels, then through one with 1.4 mm diameter, and finally through another one with 1.2 mm diameter.

[0027] If it is necessary to further reduce the liposuctioned tissue to obtain nanofat, the physician may use a single-channel 100 adipose tissue fluidization device.

[0028] According to, the single-channel adipose tissue fluidization device 100 comprises a body 150 comprising two ends 200A,B aligned along a same geometric axis, wherein each end 200A,B comprises an adipose tissue inlet / outlet connection.

[0029] The ends 20A,B of the adipose tissue fluidization device 10 comprise a connection for syringes, preferably a Luer Lock type connection.

[0030] Additionally, the adipose tissue fluidization device 100 comprises at least three meshes 250 spaced apart internally to the body 150, wherein the meshes 250 are of themeshtype and have weaves that may have the same sizes or different sizes.

[0031] Finally, the adipose tissue fluidization devices 10 with different diameters can be combined into an integrated filter set with at least 3 filters with channels of different diameters, preferably 2.4, 1.4 and 1.2 mm. Additionally, the integrated filter assembly may comprise at least one multi-channel adipose tissue fluidization device 10 and the single-channel adipose tissue fluidization device 100.

[0032] The present invention has been described based on a specific embodiment. However, one skilled in the art will note that several other embodiments and variants are possible within the scope of the claims.

Claims

Adipose tissue fluidification device (10), comprising:a body (15);two ends (20A, B) aligned along the same geometric axis; anda plurality of channels (25) internal to the body (15).Adipose tissue fluidization device (10), according to claim 1, wherein the two ends (20A,B) comprise a first end (20A) and a second end (20B), wherein each of the ends (20A,B) comprises an adipose tissue inlet / outlet connection.Adipose tissue fluidification device (10), according to claim 2, wherein each adipose tissue inlet / outlet connection is a syringe connection, preferably of the Luer Lock type.Adipose tissue fluidification device (10), according to claim 1, wherein the channels of the plurality of channels (25) have the same diameter.Adipose tissue fluidification device (10), according to claim 1, wherein the diameter of the channels of the plurality of channels (25) ranges from 1.2 to 2.4 mm, preferably 1.2, 1.4 and 2.4 mm.Adipose tissue fluidification device (100), comprising:a body (150);two ends (200A, B) aligned along the same geometric axis; andat least three meshes (250) spaced apart and internal to the body (150).Adipose tissue fluidification device (100), according to claim 6, wherein the meshes (250) are of themeshtype and have weaves with the same sizes and / or weaves with different sizes.Adipose tissue fluidization device (100), according to claim 6, wherein the two ends (200A,B) comprise a first end (200A) and a second end (200B), wherein each of the ends (200A,B) each comprises an adipose tissue inlet / outlet connection.Adipose tissue fluidification device (100), according to claim 8, wherein each adipose tissue inlet / outlet connection is a syringe connection, preferably of the Luer Lock type.Invention of product, process, System, kit, medium or use, comprising one or more elements described in the present patent application.

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