microRNA signature for the early diagnosis of localized radiation-induced lesions

Abstract

The present invention relates to an in vitro method for predicting the occurrence and / or severity of an injury induced by localized irradiation or an in vitro method for detecting localized irradiation in a subject and / or determining a level of localized irradiation in a subject, said methods comprising the following steps: a) obtaining an expression profile of at least the following 15 microRNAs in a biological sample from the subject: (i) hsa-let-7b-3p; (ii) hsa-let-7d-3p; (iii) hsa-miR-10a-5p; (iv) hsa-miR-24-3p; (v) hsa-miR-99b-5p; (vi) hsa-miR-122-5p; (vii) hsa-miR-139-5p; (viii) hsa-miR-140-3p; (ix) hsa-miR-191-5p; (x) hsa-miR-214-3p; (xi) hsa-miR-340-5p; (xii) hsa-mIR-361-5p; (xiii) hsa-miR-501-5p; (xiv) hsa-miR-652-3p; and (xv) hsa-miR-744-5p, the expression profile being previously measured in vitro from the biological sample of said subject;b) compare the microRNA expression profile obtained in step a) with at least one reference microRNA expression profile, and c) based on the comparison in step b), predict the occurrence and / or severity of injury in the subject following localized irradiation or detect localized irradiation and / or determine the level of localized irradiation.

Images