ARIH1-modified immune cells and uses thereof

Modified immune cells with reduced ARIH1 and BCL11B expression, engineered for enhanced degranulation and cytotoxicity, address persistence and rejection issues, improving therapeutic efficacy in cancer treatment.

WO2026021484A1 Publication Date: 2026-01-29PEKING UNIV +1
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Patent Information

Application Number
PCT/CN2025/110118
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-07-23
Filing Date
2025-07-23
Publication Date
2026-01-29

AI Technical Summary

Technical Problem

Allogeneic immune cells, such as CAR-T and CAR-NK cells, face challenges including poor persistence due to host immune rejection, activation-induced cell death, and graft-versus-host disease, limiting their therapeutic effectiveness in cancer treatment.

Method used

Modified immune cells with reduced expression and/or function of ARIH1 and optionally BCL11B proteins, engineered with receptors like CAR, are developed using gene editing techniques like CRISPR/Cas9, enhancing degranulation, cytotoxicity, and persistence.

Benefits of technology

The modified immune cells exhibit enhanced degranulation, cytotoxicity, and persistence, reducing host rejection and graft-versus-host disease, making them more effective in treating cancers and other conditions.

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Abstract

Provided relates to immune cells (e.g., T cells such as γδ T cells) modified to have no or reduced expression and / or function of Ariadne homolog 1 (ARIH1) and further optionally BCL11B, uses thereof (e.g., for treating glioblastoma), and methods of generating thereof. Also provided are methods of modifying γδ T cells using pseudotyped lentiviruses (e.g., BaEV-Rless glycoprotein or BaEV-TR glycoprotein pseudotyped lentiviruses).
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