Use of cas protein, and detection method and kit for target nucleic acid molecule
By immobilizing target nucleic acid primers on a solid surface, combined with specific amplification enzymes and buffer solutions, a detection system was formed that solved the false positive problem caused by aerosol contamination, achieving efficient and low-cost nucleic acid detection.
Patent Information
- Application Number
- PCT/CN2026/074591
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-12-06
- Filing Date
- 2026-01-23
- Publication Date
- 2026-06-11
AI Technical Summary
Existing molecular detection technologies are susceptible to aerosol contamination, leading to false positive results and affecting the accuracy and reliability of experimental results. This is especially true in high-sensitivity detection systems, where existing prevention and control measures are costly in terms of manpower and financial resources and have limited effectiveness.
By immobilizing target nucleic acid primers on a solid surface and combining them with specific amplification enzymes and buffers, a detection system is formed, which reduces the risk of aerosol contamination from amplification products. Detection is performed using CRISPR diagnostic technology, immunoassay technology, aptamer technology, and ribozyme technology.
It significantly reduces or eliminates the risk of aerosol contamination, improves the accuracy and reliability of detection, reduces human and financial costs, and is applicable to the detection of various nucleic acid detection methods and target nucleic acids.