Soybean GmFTL1 protein and soybean GmFTL2 protein as well as applications thereof

Abstract

The invention discloses a soybean GmFTL1 protein and a soybean GmFTL2 protein with amino acid sequences shown as SEQ ID No.1 and SEQ ID No.2 or amino acid sequences which are formed from the sequence by replacing, losing or adding one or more amino acids and have the equivalent functions. Overexpression soybean flowering genes GmTL1 and GmTL2 can remarkably stimulate the flowering of a plant (Arabidopsis), shorten the flowering time and the growth period. The invention can solve the problem of flowering asynchronism in cross breeding, the problem of growth period control of various crops, vegetables, fruits and flowers, the photoperiod sensitivity problem and the introduction problem.

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to two soybean flowering genes and their application in the regulation of plant photoperiod and flowering time. Background technique

[0002] Soybean is one of the important crops, and it is an important source of plant protein, edible oil, biodiesel, and secondary metabolites such as isoflavones and lecithin. Because soybean is a short-day plant, flowering is strictly controlled by photoperiod, so excellent varieties in different regions cannot be introduced to each other, and the growth period is also restricted by environmental photoperiod (Zhang et al., 2008). If the sensitivity of soybean to photoperiod can be reduced, and the limitation of soybean flowering to photoperiod can be broken, the problem of soybean introduction can be solved, so as to realize the mutual exchange of high-quality varieties in various regions, enrich excellent germplasm resources in various regions, ...

Examples

Embodiment 1

[0026] Example 1: Cloning of soybean flowering genes GmFTL1 and GmFTL2

[0027] GmFTL1 and GmFTL2, its gene sequence is shown in SEQ ID NO.3 and SEQ ID NO.4; the amino acid sequence of the protein encoded by it is shown in SEQ ID NO.1 and SEQ ID NO.2.

Embodiment 2

[0028] Embodiment 2: Structural analysis of soybean flowering gene GmFTL1 and GmFTL2 encoded protein (see figure 1 )

[0029] The homology between GmFTL1 and GmFTL2 protein sequences of soybean flowering gene is greater than 94.3%, and the C-terminal functional domain is highly homologous to Arabidopsis, but there are important differences. For example, the 128th amino acid residue of soybean GmFTL1 and GmFTL2 protein is glutamine (Q), the 129th is phenylalanine (F), the 132nd is arginine (R), and the 150th amino acid residue is It is leucine (L), and the corresponding parts of Arabidopsis are leucine (L), glycine (G), threonine (T) and isoleucine (I). These differences lead to higher activity of GmFTL1 and GmFTL2 than Arabidopsis FT.

Embodiment 3

[0030] Embodiment 3: Soybean flowering gene GmFTL1 and GmFTL2 plant expression vector

[0031] Soybean flowering genes GmFTL1 and GmFTL2 were constructed in figure 2 The indicated plant expression vector p35S-GW is used to overexpress soybean flowering genes GmFTL1 and GmFTL2 in plants to study their functions. The transformation method of plants is carried out by Agrobacterium-mediated method. Screening in plants is marked Bar.

technical field

[0001] The invention relates to the field of genetic engineering, in particular to two soybean flowering genes and their application in the regulation of plant photoperiod and flowering time. Background technique

[0002] Soybean is one of the important crops, and it is an important source of plant protein, edible oil, biodiesel, and secondary metabolites such as isoflavones and lecithin. Because soybean is a short-day plant, flowering is strictly controlled by photoperiod, so excellent varieties in different regions cannot be introduced to each other, and the growth period is also restricted by environmental photoperiod (Zhang et al., 2008). If the sensitivity of soybean to photoperiod can be reduced, and the limitation of soybean flowering to photoperiod can be broken, the problem of soybean introduction can be solved, so as to realize the mutual exchange of high-quality varieties in various regions, enrich excellent germplasm resources in various regions, ...

Examples

Embodiment 1

[0026] Example 1: Cloning of soybean flowering genes GmFTL1 and GmFTL2

[0027] GmFTL1 and GmFTL2, its gene sequence is shown in SEQ ID NO.3 and SEQ ID NO.4; the amino acid sequence of the protein encoded by it is shown in SEQ ID NO.1 and SEQ ID NO.2.

Embodiment 2

[0028] Embodiment 2: Structural analysis of soybean flowering gene GmFTL1 and GmFTL2 encoded protein (see figure 1 )

[0029] The homology between GmFTL1 and GmFTL2 protein sequences of soybean flowering gene is greater than 94.3%, and the C-terminal functional domain is highly homologous to Arabidopsis, but there are important differences. For example, the 128th amino acid residue of soybean GmFTL1 and GmFTL2 protein is glutamine (Q), the 129th is phenylalanine (F), the 132nd is arginine (R), and the 150th amino acid residue is It is leucine (L), and the corresponding parts of Arabidopsis are leucine (L), glycine (G), threonine (T) and isoleucine (I). These differences lead to higher activity of GmFTL1 and GmFTL2 than Arabidopsis FT.

Embodiment 3

[0030] Embodiment 3: Soybean flowering gene GmFTL1 and GmFTL2 plant expression vector

[0031] Soybean flowering genes GmFTL1 and GmFTL2 were constructed in figure 2 The indicated plant expression vector p35S-GW is used to overexpress soybean flowering genes GmFTL1 and GmFTL2 in plants to study their functions. The transformation method of plants is carried out by Agrobacterium-mediated method. Screening in plants is marked Bar.

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