An optical detection system using a continuously variable magnification lens

Abstract

The present invention discloses an optical detection system using a continuously variable magnification lens, which relates to a surface plasmon resonance optical detection system and is composed of an incident light unit, a reflected light unit, and a stage; the incident light unit and the reflected light unit are fixed on the same plane and are symmetrically arranged left and right; the optical axis of the incident light unit intersects with the optical axis of the reflected light unit and forms a plane parallel to the plane where the incident light unit and the reflected light unit are fixed; the stage is perpendicular to the plane where the incident light unit and the reflected light unit are fixed; the angle between the optical axis of the incident light unit and the stage is equal to the angle between the optical axis of the reflected light unit and the stage, and the time change of the reflectivity at a specific angle can be used as a collected signal, and the specific angle can be set by calculating the maximum difference of the resonance angle function curve of the surface plasmon resonance. Therefore, it has higher sensitivity.

Description

Technical Field

[0001] The present invention relates to a surface plasmon resonance optical detection system, and more particularly to a two-dimensional micron-level surface plasmon resonance optical detection system using a continuously variable magnification lens. Background Art

[0002] Surface Plasmon Resonance (SPR) is an optical physical phenomenon. By utilizing this optical physical phenomenon of SPR, it is possible to achieve real-time observation of surface phenomena such as molecular binding and film formation, and to give signals of non-specific binding with high sensitivity and high selectivity. In currently available SPR detection instruments on the market, the prism coupling structure mostly adopts the Kretschmann structure. When the incident angle is greater than a certain specific value, the light emitted by the laser source undergoes total internal reflection on the prism surface, and after total internal reflection, the light irradiates the optical sensor device to complete signal acquisition. Currently, most of the commercially available SPR detection instruments use photodiodes or photoresistors to detect the change in the SPR angle or the intensity change of the reflected light. Therefore, the above-mentioned SPR detection instruments detect the entire metal thin film surface as a single point, so only one-dimensional point detection can be achieved, thereby greatly increasing the detection error and usage cost of the SPR detection instrument. Some SPR detection instruments use CCD or CMOS cameras to detect SPR signals. Although this form can achieve two-dimensional detection, its optical resolution is relatively low and it cannot distinguish two sample points within 50 microns. Improving the optical resolution of SPR detection instruments has great economic value and scientific research value. For example, improving the optical resolution of SPR detection instruments can effectively increase the utilization rate of SPR detection chips, and can be applied to related research and analysis such as DNA microarrays and protein microarrays, and can significantly reduce the detection cost. In addition, improving the optical resolution of SPR detection instruments can enable related research and analysis of microorganisms or individual cells.

[0003] During the SPR detection process, in order to find the resonance angle of surface plasmon resonance under different conditions, it is necessary to continuously change the incident angle of the incident light or the reflection angle on the reflection light side. To achieve this purpose, a one-axis or two-axis rotation structure is mostly used. Limited by the current precision of mechanical manufacturing, processing, and assembly and mechanical errors, as shown in Figure 1 Figure a, when the incident light angle changes, the incident point will shift, resulting in the shift of the optical axis. Similarly, as shown in Figure 1As shown in Figure b, when the angle changes on the reflected light side, the imaging center of an optical sensor device such as a CCD or CMOS camera will also shift. The shift between the optical axis and the imaging center will ultimately lead to imaging displacement, imaging distortion, imaging blurring, and imaging loss, ultimately resulting in detection errors. The impact of the above-mentioned detection errors increases with the improvement of the optical resolution. Therefore, it is necessary to design a new three-dimensional SPR detection structure that can improve the optical resolution in SPR detection while reducing the detection errors caused by angle changes. Summary of the Invention

[0004] The main objective of the present invention is to use a continuously variable magnification lens to achieve the analysis and detection of micron-level real-time surface plasmon resonance on a two-dimensional plane. Moreover, through the sample stage, focal length adjustment slide, lens aberration correction adapter, etc., the optical axis shift and aberration caused by angle changes are effectively corrected to reduce detection errors.

[0005] The technical solution adopted by the present invention is: an optical detection system using a continuously variable magnification lens, which is composed of an incident light unit, a reflected light unit, and a sample stage; the incident light unit and the reflected light unit are fixed on the same plane and are symmetrically arranged left and right; the optical axis of the incident light unit intersects with the optical axis of the reflected light unit and forms a plane parallel to the plane where the incident light unit and the reflected light unit are fixed; the sample stage is perpendicular to the plane where the incident light unit and the reflected light unit are fixed; the angle between the optical axis of the incident light unit and the sample stage is equal to the angle between the optical axis of the reflected light unit and the sample stage;

[0006] The reflected light unit includes a continuously variable magnification lens, an optical sensor device, an optical sensor device bracket, a focal length adjustment slide, a reflected light angle slide, a reflected light fixing plate, a reflected light angle slide, and a reflected light unit slide fixing plate; a lens aberration correction adapter composed of a correction adapter and a locking ring is also installed between the continuously variable magnification lens, the optical sensor device, and the optical sensor device bracket;

[0007] The optical detection system uses a lens aberration correction adapter to correct the aberration caused by the deflection angle between the imaging plane of the optical sensor device and the plane where the detection object is located;

[0008] The external thread of the continuously variable magnification lens is connected to the internal thread of the correction adapter, and the external thread of the correction adapter is connected to the internal thread of the optical sensor device;

[0009] The continuously variable magnification lens and the optical sensor device are connected through the lens aberration correction adapter; the continuously variable magnification lens and the optical sensor device are connected to the focal length adjustment slide through the optical sensor device bracket.

[0010] Furthermore, the continuously variable magnification lens is directly mounted to the optical sensor device; when the interface specifications between the continuously variable magnification lens and the optical sensor device are different, it is mounted through a connector conversion adapter.

[0011] Furthermore, the focal length adjustment stage, the continuously variable magnification lens, and the optical sensor device are directly connected to the reflected light angle stage's reflected light fixing plate through the optical sensor device bracket; the reflected light angle stage's reflected light fixing plate carrying the accessories of the continuously variable magnification lens, the optical sensor device, the optical sensor device bracket, and the focal length adjustment stage is fixed on the reflected light angle stage.

[0012] Furthermore, the reflected light angle stage's reflected light fixing plate carrying the accessories of the continuously variable magnification lens, the optical sensor device, the optical sensor device bracket, and the focal length adjustment stage is fixed on the side of the reflected light angle stage.

[0013] Furthermore, under the condition of not affecting the angle change of the reflected light angle stage, the accessories of the continuously variable magnification lens, the optical sensor device, the optical sensor device bracket, and the focal length adjustment stage are fixed in the front, rear, upper, or lower positions of the reflected light angle stage; finally, the continuously variable magnification lens, the optical sensor device, the optical sensor device bracket, the focal length adjustment stage, the reflected light angle stage's reflected light fixing plate, and the accessories of the reflected light angle stage are fixed on the same plane as the incident light unit through the reflected light unit stage fixing plate.

[0014] Furthermore, the incident light unit includes a collimator tube, a collimator tube bracket, a polarizing filter tube, a first polarized light positioning photoelectric sensor, a second polarized light positioning photoelectric sensor, a polarizing filter tube photoelectric sensor fixing plate, a polarizing filter tube bracket, a polarizing filter tube gear, a polarizing filter motor, a polarizing filter motor bracket, a polarizing filter motor gear, an incident light fixing base plate, a polarizing filter, an incident light angle stage, and an incident light unit stage fixing plate;

[0015] The collimator tube is fixed on the incident light fixing base plate through the collimator tube bracket; the polarizing filter tube with a polarizing filter is connected to the polarizing filter tube bracket through the polarizing filter tube gear; the polarizing filter motor is connected to the polarizing filter motor bracket through the polarizing filter motor gear; the polarizing filter tube bracket with the polarizing filter tube and the polarizing filter motor gear and the polarizing filter motor bracket with the polarizing filter motor and the polarizing filter motor gear are fixed on the incident light fixing base plate.

[0016] Furthermore, the stage includes a stage panel, a stage main board, a prism, a prism fixing plate, a prism fixing plate x-axis pusher, a prism fixing plate y-axis pusher, a first spring, a second spring, a third spring, a fourth spring, a fifth spring, a sixth spring, a seventh spring, an eighth spring, an x-axis push rod motor, a y-axis push rod motor, an x-axis push rod motor bracket, a y-axis push rod motor bracket, an x-axis push rod motor pusher, and a y-axis push rod motor pusher;

[0017] The prism fixing plate with the prism, the prism fixing plate x-axis pusher, the prism fixing plate y-axis pusher, the first spring, the second spring, the third spring, the fourth spring, the fifth spring, the sixth spring, the seventh spring, and the eighth spring are installed in the corresponding grooves of the stage main board and covered by the stage panel; the x-axis push rod motor is connected to the prism fixing plate through the x-axis push rod motor bracket;

[0018] The x-axis push rod motor pusher is fixed to the stage main board, and the movement of the prism fixing plate with the prism in the X-axis direction is realized through the prism fixing plate x-axis pusher, the fifth spring, the sixth spring, the seventh spring, the eighth spring, the x-axis push rod motor, the x-axis push rod motor bracket, and the x-axis push rod motor pusher;

[0019] The y-axis push rod motor is connected to the stage main board through the y-axis push rod motor bracket, and the y-axis push rod motor pusher is fixed to the prism fixing plate; the movement of the prism fixing plate with the prism in the Y-axis direction is realized through the prism fixing plate y-axis pusher, the first spring, the second spring, the third spring, the fourth spring, the y-axis push rod motor, the y-axis push rod motor bracket, and the y-axis push rod motor pusher.

[0020] Advantages of the present invention:

[0021] The present invention can greatly improve the optical resolution of SPR detection and can achieve the conversion of multiple magnification factors according to requirements.

[0022] The present invention can effectively compensate for the optical axis offset and aberration caused by the angle change, reducing the detection error.

[0023] The present invention adopts a double-axisymmetric rotation structure to realize the transformation function of the incident angle of the incident light and the reflection angle of the reflected light. The time change of the reflectivity at a specific angle can be used as the collected signal, and the specific angle can be set by calculating the maximum difference of the resonance angle function curve of the surface plasmon resonance. Therefore, it has higher sensitivity.

[0024] In addition to the purposes, features, and advantages described above, the present invention has other purposes, features, and advantages. The present invention will be further described in detail below with reference to the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings, which form a part of this application, are used to provide a further understanding of the present invention. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention.

[0026] Figure 1 is a structural diagram of an optical detection system in the prior art;

[0027] Figure 2 is the first detection diagram of the present invention;

[0028] Figure 3 is the second detection diagram of the present invention;

[0029] Figure 4 is a schematic diagram of the overall structure of the present invention;

[0030] Figure 5 is a schematic diagram of the structure of the reflection light unit of the present invention (top view, side view, front view in sequence);

[0031] Figure 6 is a schematic diagram of the structure of the lens aberration correction adapter of the present invention (side view, cross-sectional view, assembly example diagram in sequence);

[0032] Figure 7 is a schematic diagram of the structure of the incident light unit of the present invention (top view, side view, front view in sequence);

[0033] Figure 8 is a schematic diagram of the structure of the stage of the present invention (top view with the stage panel, top view with the stage panel removed, front view, bottom view in sequence)

[0034] view, top view with the stage panel removed, front view, bottom view).

[0035] Reference numerals:

[0036] 4 - parallel light tube, 5 - parallel light tube bracket, 6 - polarized light sheet tube, 7 - first polarized light positioning photoelectric sensor, 8 - second polarized light positioning photoelectric sensor, 9 - polarized light sheet tube photoelectric sensor fixing plate, 10 - polarized light sheet tube bracket, 11 - polarized light sheet tube gear, 12 - polarized light sheet motor, 13 - polarized light sheet motor bracket, 14 - polarized light sheet motor gear, 15 - incident light fixing base plate, 16 - polarized light sheet, 17 - incident light angle slide, 18 - incident light unit slide fixing plate;

[0037] 42 - Stage panel, 43 - Stage main board, 44 - Prism, 45 - Prism fixing plate, 46 - Prism fixing plate x - axis pusher, 47 - Prism fixing plate y - axis pusher, 48 - First spring, 49 - Second spring, 50 - Third spring, 51 - Fourth spring, 52 - Fifth spring, 53 - Sixth spring, 54 - Seventh spring, 55 - Eighth spring, 56 - x - axis push rod motor, 57 - y - axis push rod motor, 58 - x - axis push rod motor bracket, 59 - y - axis push rod motor bracket, 61 - x - axis push rod motor pusher, 60 - y - axis push rod motor pusher;

[0038] 62 - Incident light unit, 63 - Reflected light unit, 64 - Stage, 65 - Continuously variable magnification lens,

[0039] 66 - Optical sensor device, 67 - Optical sensor device bracket, 68 - Focus adjustment slide, 69 - Reflected light angle slide reflected light fixing plate, 70 - Reflected light angle slide, 71 - Reflected light unit slide fixing plate, 72 - Correction joint 73 - Locking ring. Detailed implementation mode

[0040] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0041] Refer to Figures 4 to 6 As Figures 4 to 6 shown, an optical detection system using a continuously variable magnification lens is composed of three major units: an incident light unit 62, a reflected light unit 63, and a stage 64; the incident light unit 62 and the reflected light unit 63 are fixed on the same plane and are arranged symmetrically left and right; the optical axis of the incident light unit 62 intersects with the optical axis of the reflected light unit 63 and forms a plane parallel to the plane on which the incident light unit 62 and the reflected light unit 63 are fixed; the stage 64 is perpendicular to the plane on which the incident light unit 62 and the reflected light unit 63 are fixed; the angle between the optical axis of the incident light unit 62 and the stage 64 is equal to the angle between the optical axis of the reflected light unit 63 and the stage 64; the relative position between the center of the prism in the stage 64 and the intersection point of the optical axes of the incident light unit 62 and the reflected light unit 63 is determined according to the specifications of the SPR chip in the used SPR detection optical system, thereby determining the relative positions of the stage 64, the incident light unit 62, and the reflected light unit 63.

[0042] The reflected light unit includes a continuously variable magnification lens 65, an optical sensor device 66 (such as a CCD or CMOS camera), an optical sensor device bracket 67, a focal length adjustment slide 68, a reflected light angle slide reflected light fixing plate 69, a reflected light angle slide 70, and a reflected light unit slide fixing plate 71; a lens aberration correction joint composed of a correction joint 72 and a locking ring 73 can be installed between the continuously variable magnification lens 65 and the optical sensor device 66 and the optical sensor device bracket 67 according to specific requirements; the continuously variable magnification lens 65 can be directly installed on the optical sensor device 66; when the interface (Mount) specifications between the continuously variable magnification lens 65 and the optical sensor device 66 are different, it can be installed through a joint conversion adapter.

[0043] The optical detection system using the continuously variable magnification lens corrects the aberration caused by the deflection angle between the imaging plane of the optical sensor device and the plane where the detected object is located by using a lens aberration correction joint; the external thread of the continuously variable magnification lens 65 is connected to the internal thread of the correction joint 72, and the external thread of the correction joint 72 is connected to the internal thread of the optical sensor device 66.

[0044] At the same time, the continuously variable magnification lens 65 and the optical sensor device 66 can be connected through the lens aberration correction joint according to specific requirements; the continuously variable magnification lens 65, the optical sensor device 66, etc. are connected to the focal length adjustment slide 68 through the optical sensor device bracket 67.

[0045] The focal length adjustment slide 68, the continuously variable magnification lens 65, the optical sensor device 66, etc. are directly connected to the reflected light angle slide reflected light fixing plate 69 through the optical sensor device bracket 67; the reflected light angle slide reflected light fixing plate 69 carrying accessories such as the continuously variable magnification lens 65, the optical sensor device 66, the optical sensor device bracket 67, and the focal length adjustment slide 68 is fixed on the reflected light angle slide 70.

[0046] Here, in order to save system space, the reflected light angle slide reflected light fixing plate 69 carrying accessories such as the continuously variable magnification lens 65, the optical sensor device 66, the optical sensor device bracket 67, and the focal length adjustment slide 68 is fixed on the side of the reflected light angle slide 70.

[0047] Under the condition of not affecting the angular change of the reflecting light angle stage 70, the shape of the reflecting light fixing plate 69 of the reflecting light angle stage can be changed, and accessories such as the continuous zoom lens 65, the optical sensor device 66, the optical sensor device bracket 67, and the focal length adjustment stage 68 are fixed in front of, behind, above or below the reflecting light angle stage 70, etc.; finally, through the reflecting light unit stage fixing plate 71, the continuous zoom lens 65, the optical sensor device 66, the optical sensor device bracket 67, the focal length adjustment stage 68, the reflecting light fixing plate 69 of the reflecting light angle stage, the reflecting light angle stage 70 and other accessories are fixed on the same plane as the incident light unit 62.

[0048] Reference Figure 7 , such as Figure 7 As shown, the incident light unit includes a collimator tube 4, a collimator tube bracket 5, a polarizing light sheet tube 6, a first polarized light positioning photoelectric sensor 7, a second polarized light positioning photoelectric sensor 8, a polarizing light sheet tube photoelectric sensor fixing plate 9, a polarizing light sheet tube bracket 10, a polarizing light sheet tube gear 11, a polarizing light sheet motor 12, a polarizing light sheet motor bracket 13, a polarizing light sheet motor gear 14, an incident light fixing base plate 15, a polarizing light sheet 16, an incident light angle stage 17, and an incident light unit stage fixing plate 18;

[0049] The collimator tube 4 is fixed on the incident light fixing base plate 15 through the collimator tube bracket 5; the polarizing light sheet tube 6 with the polarizing light sheet 16 is connected to the polarizing light sheet tube bracket 10 through the polarizing light sheet tube gear 11; the polarizing light sheet motor 12 is connected to the polarizing light sheet motor gear 14 through the polarizing light sheet motor bracket 13; the polarizing light sheet tube bracket 10 with the polarizing light sheet tube 6 and the polarizing light sheet motor bracket 13 with the polarizing light sheet motor 12 and the polarizing light sheet motor gear 14 are fixed on the incident light fixing base plate 15.

[0050] Reference Figure 8 , such as Figure 8 As shown, the stage includes a stage panel 42, a stage main board 43, a prism 44, a prism fixing plate 45, a prism fixing plate x-axis pusher 46, a prism fixing plate y-axis pusher 47, a first spring 48, a second spring 49, a third spring 50, a fourth spring 51, a fifth spring 52, a sixth spring 53, a seventh spring 54, an eighth spring 55, an x-axis push rod motor 56, a y-axis push rod motor 57, an x-axis push rod motor bracket 58, a y-axis push rod motor bracket 59, an x-axis push rod motor pusher 61, and a y-axis push rod motor pusher 60;

[0051] The prism fixing plate 45 with the prism 44, the prism fixing plate x-axis pusher 46, the prism fixing plate y-axis pusher 47, the first spring 48, the second spring 49, the third spring 50, the fourth spring 51, the fifth spring 52, the sixth spring 53, the seventh spring 54, and the eighth spring 55 are installed in the corresponding grooves of the stage main board 43 and covered by the stage panel 42; the x-axis push rod motor 56 is connected to the prism fixing plate 45 through the x-axis push rod motor bracket 58;

[0052] The x-axis push rod motor pusher 61 is fixed to the stage main board 43, and the movement of the prism fixing plate 45 with the prism 44 in the X-axis direction is realized through the prism fixing plate x-axis pusher 46, the fifth spring 52, the sixth spring 53, the seventh spring 54, the eighth spring 55, the x-axis push rod motor 56, the x-axis push rod motor bracket 58, and the x-axis push rod motor pusher 61;

[0053] The y-axis push rod motor 57 is connected to the stage main board 43 through the y-axis push rod motor bracket 59, and the y-axis push rod motor pusher 60 is fixed to the prism fixing plate 45; the movement of the prism fixing plate 45 with the prism 44 in the Y-axis direction is realized through the prism fixing plate y-axis pusher 47, the first spring 48, the second spring 49, the third spring 50, the fourth spring 51, the y-axis push rod motor 57, the y-axis push rod motor bracket 59, and the y-axis push rod motor pusher 60.

[0054] In this system, the adjustment of the focal length is realized through the linear motion function of the focal length adjustment slide 68; therefore, the form of the focal length adjustment slide 68 is not limited to a linear slide, and it can also be realized by a combination form of a guide rail and a motor, etc.; in this system, the purpose is to change the incident light angle and the reflected light angle through the angle slide; therefore, the incident light angle and the reflected light angle can also be changed through a rotary slide.

[0055] After adjusting the relative position between the continuously variable magnification lens 65 and the optical sensor device 66, the locking ring 73 is used to fix the relative position between the continuously variable magnification lens 65 and the optical sensor device 66; and, according to requirements, the internal and external threads of the correction adapter 72 can be selected from C-MOUNT; CS-MOUNT; D-MOUNT; RMS-MOUNT; M26-MOUNT or other specifications; in addition, the adapter of the correction adapter 72 connected to the lens can also be changed to an external thread according to requirements.

[0056] In this system, in order to save system space, the polarizing plate 16 and its related accessories are selected to be installed on the incident light unit side; according to requirements, the polarizing plate 16 and its related accessories can also be installed on the reflected light unit side; for example, installed before the continuously variable magnification lens 65, built into the continuously variable magnification lens 65, or installed between the continuously variable magnification lens 65 and the optical sensor device 66.

[0057] When using a continuously variable magnification lens structure in the present invention to detect the reflected light unit of the SPR detection optical system, in order to reduce the distortion that is likely to occur during high-magnification imaging, a lens aberration correction adapter can be selected to correct the aberration caused by the angular deviation between the imaging plane of the optical sensor device and the plane where the detected object is located.

[0058] Among them, according to requirements, the internal and external threads of the lens aberration correction adapter can be selected from C-MOUNT; CS-MOUNT; D-MOUNT; RMS-MOUNT; M26-MOUNT or other specifications.

[0059] In the present invention, a double-axisymmetric rotation structure of incident light and reflected light is adopted to realize the function of changing the incident angle of incident light and the reflection angle of reflected light. The lens focus can be used as the origin of the rotation structure, or other points can be used as the origin of the rotation structure. In the present invention, a prism position correction unit is also designed to correct the optical axis deviation or the deviation of the imaging center that occurs when the incident angle of incident light or the reflection angle on the reflected light side changes.

[0060] Figure 2 When detecting the object to be detected A, the distortion generated during imaging is corrected by the angular deviation configuration of the imaging plane of the optical sensor device relative to the plane where the detected object is located. The dotted line represents the normal line, α is the refraction angle when the object to be detected A passes through the prism, and β is the apex angle of the prism. In order to prevent the distortion generated when the object to be detected A is imaged, the optical path lengths between any points on the object to be detected A and the imaging plane of the optical sensor device must be equal.

[0061] As Figure 2 shown, in order to reduce the distortion that is likely to occur during high-magnification imaging, the aberration is corrected by the angular deviation configuration of the imaging plane of the optical sensor device relative to the plane where the detected object is located. The angular deviation of the imaging plane relative to the plane where the detected object is located can be calculated by the following formula:

[0062] ,

[0063] where n is the refractive index of the prism. The angle α changes with the type of the object to be detected and the optimum value can be obtained through experiments. Similarly, when using a continuously variable magnification lens, the continuously variable magnification lens is connected to the CCD through an aberration correction adapter designed by the applicant, and continuous variable magnification is achieved through a stepper motor or a servo motor to realize various optical resolution capabilities.

[0064] Figure 3 Before (dotted line) and after (solid line) the reflection angle on the reflected light side changes, the optical path length from point A to the imaging plane will change with the change of the reflection angle.

[0065] As Figure 3As shown in the figure, when the reflection angle on the reflected light side changes, the optical path from the object to be inspected to the imaging surface will change. Therefore, when magnifying the object to be inspected at a high magnification, as the reflection angle on the reflected light side changes, the focal length of the lens must be adjusted accordingly. When using a long-distance object magnifying lens, in order to achieve the optical focusing of the focal length between different lenses, a separated structure is adopted between the lens and the optical sensor device. While achieving the optical focusing of the lens on the optical sensor device through a high-precision guide rail and a high-precision stepping motor or servo motor, it is also possible to compensate for the change in the focal length caused by the change in the reflection angle on the reflected light side. When the optical path is perpendicular to the side surface of the prism and reaches the imaging surface (dashed line), the optical path length from point A to the imaging surface is L0, the optical path length from point A to point B on the side surface of the prism is l, the focal length is f, and the length of the image distance b0 at this time is:

[0066] ,

[0067] When the reflected light side moves to a refraction angle of α, the length of the optical path L1 at this time is:

[0068] ,

[0069] Therefore, the compensation value for the change in the focal length by the high-precision stepping motor or servo motor can be calculated by the following formula:

[0070] ,

[0071] In the present invention, a stage capable of moving in the xy plane can be realized. In addition to the function of moving the object to be inspected, the stage can also realize the function of compensating for the detection error caused by the change in the incident angle of the incident light or the reflection angle on the reflected light side. When the incident angle of the incident light changes or the reflection angle on the reflected light side changes, resulting in the deviation of the optical axis, the optical axis detected by an optical sensor device such as a CCD or CMOS camera will also deviate. At this time, the prism stage is moved by the push rod motors on the x-axis and y-axis of the stage, and the optical axis positions before and after the change in the incident angle and reflection angle are overlapped to compensate for the detection error caused by the change in the incident angle of the incident light or the reflection angle on the reflected light side. To sum up, in the present invention, the applicant has designed a new three-dimensional SPR detection structure that can improve the optical resolution in SPR detection while reducing the detection error caused by the angle change.

[0072] In the present invention patent, first, an optical unit composed of a single or plural lenses is added between the prism and the optical sensor device (CCD) in the surface plasmon resonance (SPR) detection technology to improve the optical resolution of SPR detection. And, a multi-point positioning switching turntable is designed to realize the switching between optical units with various optical resolutions. In addition, a multi-axis cooperation structure is also designed to reduce the detection error.

[0073] The present invention can greatly improve the optical resolution of SPR detection and can achieve the conversion of multiple magnification ratios according to requirements.

[0074] The present invention can effectively compensate for the optical axis offset and aberration caused by the angle change, and reduce the detection error.

[0075] The present invention adopts a double-axisymmetric rotation structure to realize the transformation function of the incident angle of incident light and the reflection angle of reflected light. The time change of the reflectivity at a specific angle can be used as the collected signal, and the specific angle can be set by calculating the maximum difference of the resonance angle function curve of surface plasmon resonance. Therefore, it has higher sensitivity.

[0076] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An optical detection system using a continuously variable magnification lens, characterized in that, it is composed of an incident light unit (62), a reflected light unit (63), and a stage (64); the incident light unit (62) and the reflected light unit (63) are fixed on the same plane and arranged symmetrically left and right; the optical axis of the incident light unit (62) intersects with the optical axis of the reflected light unit (63) and forms a plane parallel to the plane fixing the incident light unit (62) and the reflected light unit (63); the stage (64) is perpendicular to the plane fixing the incident light unit (62) and the reflected light unit (63); the angle between the optical axis of the incident light unit (62) and the stage (64) is equal to the angle between the optical axis of the reflected light unit (63) and the stage (64); the said reflected light unit includes a continuously variable magnification lens (65), an optical sensor device (66), an optical sensor device bracket (67), a focal length adjustment slide (68), a reflected light angle slide reflected light fixing plate (69), a reflected light angle slide (70), a reflected light unit slide fixing plate (71); the external thread of the continuously variable magnification lens (65) is connected to the internal thread of the correction joint (72), and the external thread of the correction joint (72) is connected to the internal thread of the optical sensor device (66); the continuously variable magnification lens (65) and the optical sensor device (66) are connected through the correction joint (72); the continuously variable magnification lens (65) and the optical sensor device (66) are connected to the focal length adjustment slide (68) through the optical sensor device bracket (67); a lens aberration correction joint composed of a correction joint (72) and a locking ring (73) is also installed between the continuously variable magnification lens (65), the optical sensor device (66), and the optical sensor device bracket (67); the said incident light unit includes a collimator tube (4), a collimator tube bracket (5), a polarizing filter tube (6), a first polarized light positioning photoelectric sensor (7), a second polarized light positioning photoelectric sensor (8), a polarizing filter tube photoelectric sensor fixing plate (9), a polarizing filter tube bracket (10), a polarizing filter tube gear (11), a polarizing filter motor (12), a polarizing filter motor bracket (13), a polarizing filter motor gear (14), an incident light fixing base plate (15), a polarizing filter (16), an incident light angle slide (17), an incident light unit slide fixing plate (18); the collimator tube (4) is fixed on the incident light fixing base plate (15) through the collimator tube bracket (5); the polarizing filter tube (6) with a polarizing filter (16) is connected to the polarizing filter tube gear (11) and fixed on the polarizing filter tube bracket (10); the polarizing filter motor (12) and the polarizing filter motor gear (14) are connected to the polarizing filter motor bracket (13); the polarizing filter tube bracket (10) with the polarizing filter tube (6) and the polarizing filter tube gear (11) and the polarizing filter motor bracket (13) with the polarizing filter motor (12) and the polarizing filter motor gear (14) are fixed on the incident light fixing base plate (15).

2. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, the optical detection system corrects the aberration caused by the deflection angle between the imaging surface of the optical sensor device and the plane where the detected object is located by using a lens aberration correction adapter.

3. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, the continuously variable magnification lens (65) is directly mounted to the optical sensor device (66); when the interface specifications between the continuously variable magnification lens (65) and the optical sensor device (66) are different, it is mounted through an adapter conversion adapter.

4. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, a focal length adjustment slide table (68), the continuously variable magnification lens (65) and the optical sensor device (66) are directly connected to the reflected light fixed plate (69) of the reflected light angle slide table through the optical sensor device bracket (67); the reflected light fixed plate (69) of the reflected light angle slide table carrying the accessories of the continuously variable magnification lens (65), the optical sensor device (66), the optical sensor device bracket (67), and the focal length adjustment slide table (68) is fixed on the reflected light angle slide table (70).

5. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, the reflected light fixed plate (69) of the reflected light angle slide table carrying the accessories of the continuously variable magnification lens (65), the optical sensor device (66), the optical sensor device bracket (67), and the focal length adjustment slide table (68) is fixed on the side of the reflected light angle slide table (70).

6. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, under the condition of not affecting the angle change of the reflected light angle slide table (70), the accessories of the continuously variable magnification lens (65), the optical sensor device (66), the optical sensor device bracket (67), and the focal length adjustment slide table (68) are fixed in the front, rear, upper or lower positions of the reflected light angle slide table (70); finally, the accessories of the continuously variable magnification lens (65), the optical sensor device (66), the optical sensor device bracket (67), the focal length adjustment slide table (68), the reflected light fixed plate (69) of the reflected light angle slide table, and the reflected light angle slide table (70) are fixed on the same plane as the incident light unit (62) through the reflected light unit slide table fixed plate (71).

7. The optical detection system using a continuously variable magnification lens according to claim 1, wherein, The stage includes a stage panel (42), a stage main board (43), a prism (44), a prism fixing plate (45), a prism fixing plate x-axis pusher (46), a prism fixing plate y-axis pusher (47), a first spring (48), a second spring (49), a third spring (50), a fourth spring (51), a fifth spring (52), a sixth spring (53), a seventh spring (54), an eighth spring (55), an x-axis push rod motor (56), a y-axis push rod motor (57), an x-axis push rod motor bracket (58), a y-axis push rod motor bracket (59), an x-axis push rod motor pusher (61), and a y-axis push rod motor pusher (60); The prism fixing plate (45) with the prism (44) is installed in corresponding grooves of the stage main board (43) together with the prism fixing plate x-axis pusher (46), the prism fixing plate y-axis pusher (47), the first spring (48), the second spring (49), the third spring (50), the fourth spring (51), the fifth spring (52), the sixth spring (53), the seventh spring (54), and the eighth spring (55), and is covered by the stage panel (42); the x-axis push rod motor (56) is connected to the prism fixing plate (45) through the x-axis push rod motor bracket (58); The x-axis push rod motor pusher (61) is fixed to the stage main board (43), and the movement of the prism fixing plate (45) with the prism (44) in the X-axis direction is realized through the prism fixing plate x-axis pusher (46), the fifth spring (52), the sixth spring (53), the seventh spring (54), the eighth spring (55), the x-axis push rod motor (56), the x-axis push rod motor bracket (58), and the x-axis push rod motor pusher (61); The y-axis push rod motor (57) is connected to the stage main board (43) through the y-axis push rod motor bracket (59), and the y-axis push rod motor pusher (60) is fixed to the prism fixing plate (45); the movement of the prism fixing plate (45) with the prism (44) in the Y-axis direction is realized through the prism fixing plate y-axis pusher (47), the first spring (48), the second spring (49), the third spring (50), the fourth spring (51), the y-axis push rod motor (57), the y-axis push rod motor bracket (59), and the y-axis push rod motor pusher (60).

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