A blood cell analyzer

Abstract

The present application discloses a blood cell analyzer. The blood cell analyzer includes a sample processing plate, an amplifying and counting plate, and a blood cell detector; the sample processing plate is used to hold a blood cell sample; the amplifying and counting plate includes a lens; the blood cell detector includes an image sensor; the sample processing plate is detachably mounted on the amplifying and counting plate, and the amplifying and counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample magnified by the lens is transmitted to the image sensor. The blood cell analyzer provided by the present application is a disposable, one-time use magnifying glass that meets clinical inspection standards and ensures the reliability of the results. The analyzer is low in cost and can be used in daily environments.

Description

Technical Field

[0001] The present application relates to a blood cell analyzer, belonging to the technical field of medical instruments. Background Art

[0002] In the field of modern clinical laboratory medicine, routine blood tests are one of the most commonly used, important and basic test items for disease diagnosis and health examinations. Through this examination, we can understand the size, number, shape, proportion and other data of various cells in human blood, so as to provide clinicians with diagnosis and treatment of diseases and health indicators for the physical examination population.

[0003] Currently, there are two main methods for routine blood tests: manual inspection and instrument inspection.

[0004] Manual inspection method: quantitatively draw blood samples, dilute with diluent or hemolytic agent, and inject them into the modified Neubauer blood cell counting plate. Count the number of various blood cells under the microscope according to different requirements, and then calculate the number of various cells in a certain volume. At the same time, push the same blood sample into a blood film on a slide, fix and stain it, and classify the white blood cells according to different forms under a biological microscope. The advantage of this method is that it can be counted and classified by the naked eye, and the results are relatively reliable and accurate. The disadvantages are slow speed, low efficiency, cumbersome operation, and the existing biological microscope needs to be reused, and professionals need to repeatedly adjust the objective lens, field of view, focal length, etc. The use of different personnel will affect the accuracy of the analysis results. However, this method is still reserved for re-examination when the results of the instrument method are abnormal, or for special cases with special requirements and where there is no condition to purchase instruments.

[0005] Instrument inspection method: Blood cell analyzers can be roughly divided into semi-automatic, fully automatic, two-classification, three-classification, and five-classification according to the complexity of the instrument structure. The last two instruments are currently the most commonly used. First, the instrument automatically or semi-automatically absorbs anticoagulated blood samples, and then automatically distributes the samples to different containers for dilution, hemolysis, and staining. Through the red blood cell and platelet channels, the Coulter principle is used to determine the number of resistance pulses and pulse sizes of red blood cells and platelets, and the number of red blood cells and platelets is counted. Through the white blood cell channel, the Coulter principle is used to determine the number of white blood cell resistance pulses and pulse sizes, and the volume counts the number of white blood cells. At the same time, laser, visible light, cell staining and other technologies are used to classify and count white blood cells. Its advantages are simple operation, fast measurement speed, high degree of automation, and the number of counted cells is much higher than the manual method, so the counting result has a high accuracy rate. The disadvantages are that the instrument is precise and expensive, the operating environment requirements are high, daily maintenance is frequent, and the accuracy of cell morphology recognition is lower than the manual method. Summary of the invention

[0006] According to the present application, a blood cell analyzer is provided, in which the magnifying counting plate has two structures. The biconvex spherical lens of the first magnifying counting plate is installed in the conical through hole of the counting magnifying plate, and the lens of the second magnifying counting plate is an integrated plano-convex lens fixed on the outer bottom surface of the main plate in the magnifying counting plate. They are disposable, one-time-use microscope magnifiers with set parameters such as field of view, focal length, magnification, etc., which meet clinical inspection standards, ensure the reliability of results, and can be used by non-professionals for fast and efficient detection and analysis. In addition, the analyzer has low cost and can be used in daily environments.

[0007] A blood cell analyzer, comprising a sample processing plate, an amplification counting plate and a blood cell detector;

[0008] The sample processing plate is used to hold blood cell samples;

[0009] The magnifying counting plate comprises a lens;

[0010] The blood cell detector includes an image sensor;

[0011] The sample processing plate is detachably mounted on the amplifying and counting plate, and the amplifying and counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample magnified by the lens is transmitted to the image sensor.

[0012] Optionally, the sample processing plate comprises a glass slide, a support member and a cover glass;

[0013] A concave sample pool is provided on the glass slide;

[0014] The cover glass and the support are both located in the sample pool, and a gap for containing the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the support.

[0015] Optionally, the height of the gap along the vertical direction is 0.05-0.12 mm.

[0016] Optionally, the amplification counting plate further comprises a main body plate;

[0017] The main body plate is provided with a groove for fixing the sample processing plate;

[0018] The lens is fixed below the groove through the main body plate.

[0019] Optionally, the main body plate is provided with a conical through hole in the vertical direction and connected with the groove, the lens is a biconvex spherical lens, the biconvex spherical lens is fixed in the through hole, and the through hole gradually decreases in diameter in the vertical direction.

[0020] Optionally, the diameter of the large-diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm; and the height of the through hole along the axial direction is 1-5 mm.

[0021] Preferably, the diameter of the biconvex spherical lens is in the range of 0.5 to 6 mm.

[0022] Optionally, the lens is a plano-convex lens, the planar end of the plano-convex lens is fixed to the outer bottom surface of the main body plate, and the convex end of the plano-convex lens is close to the cell detector.

[0023] Optionally, the thickness of the plano-convex lens along the vertical direction is 0.05-2 mm, the diameter range of the plano-convex lens is 0.3-6 mm; the thickness of the main body plate is 1-5 mm.

[0024] Optionally, the blood cell detector further comprises a housing, an upper cover plate and a wireless transmitter;

[0025] The upper surface of the housing is provided with a concave sample stage for placing the amplifying counting plate;

[0026] The sample stage is provided with a light-transmitting hole matched with the lens in the magnifying counting plate;

[0027] The image sensor and the wireless transmitter are located in a cavity enclosed by the shell, and the image sensor is electrically connected to the wireless transmitter;

[0028] The image sensor is adapted to the light-transmitting hole;

[0029] The upper cover plate is located on the upper surface of the shell, and a light source is installed on a surface of the upper cover plate facing the shell.

[0030] Optionally, the blood cell analyzer further comprises a blood collector, which comprises a tube body, a stopper, an automatic quantitative blood collection tube, a sample adding tube and a sealing sleeve;

[0031] One end of the tube body along the axial direction is open, and the sealing plug is detachably mounted on the open end of the tube body;

[0032] The automatic quantitative blood collection tube and the sample adding tube are respectively fixed on both sides of the plug along the axial direction, and the automatic quantitative blood collection tube is located in the tube body;

[0033] The automatic quantitative blood collection tube is connected to the sample adding tube;

[0034] The sealing sleeve is installed at one end of the sample adding tube.

[0035] Preferably, the blood cell analyzer further comprises a data analyzer, and the data analyzer comprises a data receiver, a data processor and a display screen;

[0036] The data receiver receives the data signal from the wireless transmitter;

[0037] The data receiver is electrically connected to the data processor;

[0038] The data processor is electrically connected to the display screen.

[0039] In this application, "the diameter of the large diameter end of the through hole" refers to the maximum value of the inner diameter of the tapered through hole;

[0040] The “diameter of the small-diameter end of the through hole” refers to the minimum value of the inner diameter of the tapered through hole.

[0041] The beneficial effects of this application include:

[0042] (1) The blood cell analyzer provided by the present application is a wireless handheld intelligent blood cell analyzer. Blood cell microscopy is the gold standard for clinical blood testing. The lens in the central area of ​​the counting magnifying plate groove in the present invention is a micron-level multi-specification optical magnifier with a magnification ranging from 50 times to 500 times. The magnification is determined by the diameter and focal length of the lens. It is a magnifier with a set field of view, focal length, magnification, and resolution. It can replace a biological microscope and is a disposable one-time use magnifier that meets clinical testing standards and ensures the reliability of the results. This method is not used in manual and instrumental blood cell detection methods.

[0043] (2) It is cordless and wireless, and can be held in the palm of your hand. It uses a flexible mobile terminal to install software to analyze the results. Therefore, in terms of the overall product structure design, the exquisite and compact blood cell detector is used in conjunction with mobile terminals such as mobile phones. It can be placed in the hand or pocket and is not affected by power supply restrictions, electromagnetic interference, temperature and humidity, achieving the purpose of mobile Internet + diagnosis. This structure is not used in current manual and instrumental cell detection.

[0044] (3) Intelligent operation, can be used by non-professionals, and with the characteristics of portability, instant, reliability, and Internet, it can be used in many scenarios, such as disease control emergency, accident and disaster rescue, field medical treatment, home medical treatment for special populations, routine inspections in village clinics and community health stations, hospital emergency, emergency use in other departments of the hospital, home self-inspection, etc., breaking through the limitation that traditional manual and instrumental methods can only be used in fixed laboratories, changing the scene mode of medical inspection, catering to the policy of Internet + medical care, and bringing great social value to emergency rescue, mobile medical care, home pre-inspection of common fever patients, and antibiotic management. The social value brought by this change in usage scenarios has not been realized in the current manual and instrumental blood cell testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] FIG. 1 ( a - 1 ) is a top view of a sample processing plate in one embodiment of the present application;

[0046] FIG. 1 ( a - 2 ) is a side view of a sample processing plate in one embodiment of the present application;

[0047] FIG2 (a-1) is a side view of an enlarged counting plate in the first embodiment of the present application;

[0048] FIG2 (a-2) is a top view of the enlarged counting plate in the first embodiment of the present application;

[0049] FIG2( b-1 ) is a side view of an enlarged counting plate in a second embodiment of the present application;

[0050] FIG2(b-2) is a top view of an enlarged counting plate in a second embodiment of the present application;

[0051] FIG3( a ) is a schematic diagram of the structure of a blood sampling device in one embodiment of the present application;

[0052] FIG3( b ) is a diagram of a first use state of a blood sampling device in one embodiment of the present application;

[0053] FIG3( c ) is a diagram of a second use state of a blood sampling device in one embodiment of the present application;

[0054] Figure 4 This is a structural diagram of a blood cell detector in one embodiment of the present application;

[0055] Figure 5 This is a schematic diagram of the internal structure of a blood cell detector in one embodiment of the present application;

[0056] Figure 6 This is a schematic diagram of the structure of a data analyzer in one embodiment of the present application.

[0057] List of parts and reference numerals:

[0058] 101 magnifying counting plate; 1011 main body plate; 1012 lens;

[0059] 1013 groove; 1014 through hole;

[0060] 102 sample processing plate; 1021 glass slide 1022 sample pool;

[0061] 1023 supporting; 1024 covering glass;

[0062] 200 blood collection device; 201 tube body; 202 plug;

[0063] 203 automatic quantitative blood collection tube; 204 sample adding tube; 205 sealing sleeve;

[0064] 300 blood cell detector; 301 housing; 302 image sensor;

[0065] 303 wireless transmitter; 304 upper cover plate; 305 sample stage;

[0066] 306 light transmission hole; 307 light source;

[0067] 400 data analyzer; 401 display screen;

[0068] 1. Metal sealing film; 2. Reagent tube. DETAILED DESCRIPTION

[0069] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0070] A blood cell analyzer comprises a sample processing plate, an amplifying and counting plate and a blood cell detector; the sample processing plate comprises a glass slide, a sample pool, a supporting column and a cover glass; the amplifying and counting plate comprises a groove for placing the sample processing plate and a double convex spherical lens fixed in a conical through hole in the center of the groove or an integral outwardly convex plano-convex lens fixed at the bottom of a main body plate; the blood cell detector comprises an image sensor; the sample processing plate is detachably placed in a groove matched with the amplifying and counting plate; the amplifying and counting plate is detachably mounted in the blood cell detector so that the optical image of the blood cell sample amplified by the lens is transmitted to the image sensor.

[0071] The blood cell analyzer provided by the present application performs flow control distribution processing on the sample by the sample processing board, amplifies the blood cell sample by amplification counting, and then transmits the amplified optical image to the image sensor. The sample processing board and the amplification counting board can be detachably installed in the blood cell detector, and the sample amplification counting component and the amplification counting board after detection can be discarded, achieving the effect of disposable use of blood cell analysis and ensuring the reliability of the test results.

[0072] Optionally, the sample processing plate includes a glass slide, a sample pool, a support, and a cover glass. The cover glass is fixed in the groove in the horizontal direction, and a gap for holding the blood cell sample is formed between the cover glass and the bottom surface of the groove through the support. A groove matching the sample processing plate is provided in the middle area of ​​the magnifying and counting plate, and a conical through hole with a large diameter at the end of the groove is provided on the main plate corresponding to the central area of ​​the groove, and a double convex spherical lens is fixed in the conical through hole; or an integrated exposed plano-convex lens is provided on the bottom surface of the main plate corresponding to the central area of ​​the groove; the sample processing plate is placed in the groove of the magnifying and counting plate.

[0073] Specifically, the support member of the sample processing board can be a plurality of support columns, one end of the support column is fixed to the bottom surface of the sample pool, and the other end of the support column is fixed to the cover glass, and a gap for holding the blood sample is formed between the cover glass and the bottom surface of the sample pool, and the gap makes the blood sample flattened into a single cell layer, thereby preventing the cell stacking from affecting the imaging effect and the accuracy of data analysis. A groove is provided in the middle area of ​​the magnifying counting board, and the shape and size of the groove are adapted to the sample processing board. There can be two types of magnifying counting boards, one of which is a main body plate corresponding to the central area of ​​the groove, and a conical through hole is provided in the vertical direction, and the large diameter is at the end of the groove, forming a conical through hole in the shape of an inverted triangle, and a double convex spherical lens is fixed in the conical through hole; the through hole is designed to be in the shape of an inverted triangular cone, and its function is: on the one hand, it can support the double convex spherical lens for fixing, and on the other hand, the small diameter serves as the diaphragm of the double convex spherical lens, and the size of the diaphragm affects the brightness of the image, and the diaphragm can correct the phase difference and thus affect the resolution of the image, and the diameter and focal length of the lens determine the magnification. Another type of magnifying counting plate is provided with an integrated plano-convex lens on the bottom surface of the main plate corresponding to the central area of ​​the groove. The diameter and focal length of the plano-convex lens determine the magnification and resolution. The plano-convex lens is also provided with an aperture.

[0074] The lens in the central area of ​​the counting magnifying plate in the present application is a micron-level multi-specification optical magnifying glass with magnifications ranging from 50 times to 500 times. It is a disposable magnifying glass that can replace biological microscopes and meets clinical inspection standards and ensures the reliability of results. This use has never been used in manual and instrumental blood cell inspection methods.

[0075] Optionally, the height of the gap between the cover glass and the bottom surface of the groove in the vertical direction is 0.05-0.12 mm. This gap height can make the blood sample flattened into a better single cell layer.

[0076] Specifically, the gap formed between the cover glass and the bottom surface of the groove for holding the blood sample can make the blood sample into a single cell layer membrane, preferably with a thickness of 0.1 mm. If the thickness of the gap is too small, the support column is not easy to prepare, and if the thickness of the gap is too large, the cells will overlap.

[0077] Optionally, the diameter of the large-diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm.

[0078] Specifically, the small diameter end of the through hole is equivalent to an aperture structure, and the aperture can increase the brightness of the image, and the aperture can correct the phase difference to improve the resolution of the image.

[0079] Optionally, the diameter ratio between the large diameter end and the small diameter end of the through hole is 10:1.

[0080] Optionally, the height of the through hole along the axial direction is 1 to 5 mm. Specifically, when the height of the through hole along the axial direction is 1 to 5 mm, the focal length range can be met.

[0081] Optionally, the spherical lens is a biconvex lens, which can meet the requirements of processing and preparation.

[0082] Optionally, the diameter of the biconvex spherical lens is in the range of 0.5 to 6 mm. Specifically, when the diameter of the biconvex spherical lens is in the range of 0.5 to 6 mm, the magnification can be within the range of 50 to 500 times.

[0083] Optionally, the lens is a plano-convex lens, the planar end of the plano-convex lens is fixed to the outer bottom surface of the main body plate, and the convex end of the plano-convex lens is close to the cell detector.

[0084] Optionally, the thickness of the plano-convex lens along the vertical direction is 0.05-2 mm, the diameter range of the plano-convex lens is 0.3-6 mm; the thickness of the main body plate is 1-5 mm.

[0085] Specifically, when the thickness of the plano-convex lens along the vertical direction is 0.05-2 mm, the diameter range of the plano-convex lens is 0.3-6 mm, which can meet the magnification range of 50-500 times, and when the thickness of the main plate is 1-5 mm, it can meet the focal length range.

[0086] The blood cell detector also includes a shell, a wireless transmitter and an upper cover plate; a concave sample table for placing an amplifying and counting component is provided on the upper surface of the shell; a light-transmitting hole matching the lens on the amplifying and counting plate is provided on the sample table; an image sensor and a wireless transmitter are located in a cavity enclosed by the shell, and the image sensor is electrically connected to the wireless transmitter; the image sensor is matched with the light-transmitting hole; the upper cover plate is located on the upper surface of the shell, and a light source is installed on the side of the upper cover plate facing the shell.

[0087] Specifically, the sample stage and the amplifying and counting board are matched with each other. During blood analysis, the amplifying and counting board is inserted on the sample stage.

[0088] Optionally, the blood cell analyzer further comprises a blood collector, which comprises a tube body, a plug, an automatic quantitative blood collection tube, a sample adding tube and a sealing sleeve; the tube body is open at one end along the axial direction, and the plug is detachably mounted at one end of the tube body; the automatic quantitative blood collection tube and the sample adding tube are respectively fixed at two ends of the plug along the axial direction, and the automatic quantitative blood collection tube is located in the tube body; the automatic quantitative blood collection tube is connected to the sample adding tube; and the sealing sleeve is mounted at one end of the sample adding tube. The blood collector has a simple structure and is easy to use.

[0089] Specifically, the sample adding tube is used to add the blood sample in the tube body into the amplification and counting component, and the sealing sleeve is used to ensure that the blood sample is not contaminated.

[0090] Optionally, the blood cell analyzer further comprises a data analyzer, which comprises a data receiver, a data processor and a display screen;

[0091] The data receiver receives the data signal from the wireless transmitter;

[0092] The data receiver is electrically connected to the data processor;

[0093] The data processor is electrically connected to the display screen.

[0094] The application provides a novel intelligent mobile blood cell analyzer, which uses a quantitative capillary pipette (automatic quantitative blood collection tube) that is sterilized in advance and stored in a sealed plastic tube (tube body of a blood collector) to absorb finger blood, and then puts it into another plastic tube pre-added with reagents (as shown in FIG. 3 (c)), mixes it and drips it into the sample pool of the sample processing board, puts the sample processing board into the groove of the amplifying counting board, puts the amplifying counting board into the blood cell detector, and the light emitted by the light source lamp in the blood cell detector passes through the sample and the amplifying counting board, and the optical image of the blood cells magnified by the lens at the bottom of the amplifying counting board is projected onto the photosensitive surface of the photoelectric sensor (i.e., the image sensor), and then the optical image is converted into a digital signal through the photoelectric sensor and A / D conversion, and is transmitted to the data analyzer of the mobile terminal through the wifi wireless transmitter. The blood cell image is displayed in real time on the screen of the data analyzer, and at the same time, the image algorithm analysis software in the analyzer instantly analyzes the number, morphology and classification data of white blood cells. The data can be stored in the analyzer, wirelessly transmitted to a specific data terminal, or printed through a wireless printer. The analysis results are uploaded to the medical Internet cloud platform in real time.

[0095] The following are possible implementations

[0096] A wireless handheld intelligent blood cell analyzer uses a blood collector 200 to collect finger blood, put it into a pre-filled reagent tube 2, mix it and inject it into a sample processing board 102, put an amplifying counting board 101 into a blood cell detector 300, and obtain a blood cell image by amplifying the imaging through a lens 1012 in the amplifying counting board 101. The optical image is converted into an electrical signal through an image sensor 302, and the electrical signal is converted into a digital signal through A / D conversion, and transmitted to a data analyzer 400 through a wireless transmitter 303. The algorithm analysis software installed in the data terminal is used to analyze the size, number and morphology of blood cells and other parameters to obtain a result report of platelets, red blood cells, white blood cells and white blood cell classification. The result report will be displayed and stored in the data analyzer 400, and the user can directly read the result on the display screen 401 of the data analyzer 400. The measurement result can be printed out as a report sheet wirelessly, can be transmitted to other specific terminals through Wifi, and can be uploaded to the medical Internet cloud platform in real time for use by clinicians and shared data centers. The above blood cell detector 300 is a cordless, wireless, palm-held device, and the data analyzer 400 can be various mobile terminals, such as a mobile phone, a tablet computer, a notebook computer, a mobile microcomputer, etc.

[0097] Preferably, the blood collector 200 is composed of a plastic tube (i.e., tube body 201) and a plastic plug 202. An automatic quantitative blood collection tube 203 with a capacity of 0.5-30ul is installed under the plug 202. A sample adding tube 204 is connected to the top of the plastic tube cap. A plastic sealing sleeve 205 with one end sealed is installed on the top of the sample adding tube 204. The material of the automatic quantitative blood collection tube 203 is glass or plastic. The automatic quantitative blood collection tube 203 can be independently installed on the plug 202. The automatic quantitative blood collection tube 203 can also be integrally formed with the plug 202 using an injection molding process.

[0098] Preferably, diluent, hemolysin, dye and other reagents are pre-added into the plastic tube (ie, the tube body 201 ), and the tube opening is sealed with a metal sealing film 1 .

[0099] Preferably, the sample processing plate 102 is made of plastic injection molding, and includes a glass slide 1021, a concave sample pool 1022 on the upper part of the glass slide 1021, four support columns 1023 with a height of 0.1 mm on the sample pool 1022, and virtual fine lines for identifying different areas are engraved in the central area of ​​the bottom of the sample pool 1022. Specifically, the shape of a rectangle is drawn by software on the electronic image after optical magnification transmission, which serves as the effective range for blood sample cell counting. A cover glass 1024 is fixed on the support column 1023. The magnifying counting plate 101 includes a main plate 1011, a groove 1013 is formed on the upper surface of the main plate 1011, and the main plate 1011 corresponding to the central area of ​​the groove 1013 has a conical hole (i.e., a through hole 1014), whose aperture range is 0.3-3mm, and a lens 1012 is placed in the middle of the hole, and the diameter range of the lens 1012 is 0.5-6mm. The lens material can be glass, plastic or other transparent materials, and the lens can be spherical, and the spherical lens can be biconvex or planoconvex. In order to prevent cross-contamination of cells and cross-infection of pathogens, and also to facilitate instant use in multiple scenes, the magnifying counting plate 101 is designed as a disposable piece of various specifications.

[0100] Preferably, the blood cell detector 300 has a light source 307 on its upper cover plate 304 to illuminate the cells in the blood sample under the cover glass 1024 placed in the sample processing plate 102, and the image is magnified by the lens 1012 with a magnification of 5 to 500 times, and the optical image of the cells is projected onto the photosensitive surface of the image sensor 302, and then the optical image is converted into an electrical signal by the sensor, and the electrical signal is converted into a digital signal through A / D conversion, and sent to the data analyzer 400 for processing through the wireless transmitter 303, and finally converted into a digital image and displayed on the touch screen (i.e., display screen 401) of the mobile terminal - data analyzer 400, and medical data processing and analysis are performed through algorithm software.

[0101] Preferably, the image sensor 302 adopts a CCD or CMOS chip.

[0102] Preferably, the wireless transmitter 303 adopts a wifi or bluetooth module.

[0103] Preferably, the data analyzer 400 uses a mobile terminal, which can be a mobile phone, tablet computer, laptop computer, mobile microcomputer, etc., and can be used under Windows and Android systems. When Windows system is selected, the computer uses the installed image algorithm analysis software to process and analyze the medical data of cells. When Android system is selected, the mobile phone installs a dedicated App software for image algorithm analysis to process and analyze the medical data of cells. The analysis results are uploaded to the medical Internet cloud platform in real time.

[0104] Example 1

[0105] FIG. 1(a-1) is a top view of the sample processing plate in the present embodiment, and FIG. 1(a-2) is a side view of the sample processing plate in the present embodiment; FIG. 2(a-1) is a side view of the magnifying counting plate in the present embodiment, and FIG. 2(a-2) is a top view of the magnifying counting plate in the present embodiment; FIG. 3(a) is a schematic diagram of the structure of the blood collector in the present embodiment, and FIG. 3(b) is a diagram of the first use state of the blood collector in the present embodiment; and FIG. 3(c) is a diagram of the second use state of the blood collector in the present embodiment. Figure 4 is a structural diagram of the blood cell detector in this embodiment, Figure 5 is a schematic diagram of the internal structure of the blood cell detector in this embodiment, Figure 6 The structure diagram of the data analyzer in this embodiment is shown below in conjunction with FIG. Figure 6 This embodiment is described in detail.

[0106] As shown in FIG. 1(a-1) and FIG. 1(a-2), the blood cell analyzer includes a sample processing plate 102 (collectively denoted as 102), and the sample processing plate 102 includes a glass slide 1021, a sample pool 1022, four support columns 1023 and a cover glass 1024. The four support columns 1023 are respectively located at four symmetrically distributed edge positions of the sample pool 1022, and a square cover glass 1024 is fixed on the support columns 1023 in a horizontal direction. A gap for holding a blood sample is formed between the cover glass 1024 and the bottom surface of the sample pool 1022.

[0107] As shown in Figures 2(a-1) and 2(a-2), the blood cell analyzer also includes an enlarged counting plate 101 (referred to as 101 as a whole); the enlarged counting plate 101 includes a main body plate 1011 and a biconvex lens 1012, a square (or circular) groove 1013 is provided on the main body plate 1011, a conical through hole 1014 connected to the groove 1013 is provided on the bottom surface of the groove 1013 in the vertical direction, the biconvex lens 1012 is placed in the through hole 1014, the large diameter end of the through hole 1014 is close to the groove 1013, and the small diameter end of the through hole 1014 is away from the groove 1013.

[0108] The height of the gap along the vertical direction is 0.1 mm, the diameter of the large diameter end of the through hole 1014 is 3 mm, the diameter of the small diameter end of the through hole 1014 is 0.3 mm, the height of the through hole 1014 along the axial direction is 2 mm, and the lens 1012 is a double convex lens with a diameter of 0.8 mm.

[0109] As shown in FIG3(a), the blood cell analyzer further includes a blood collector 200 (represented as 200 as a whole), which includes a tube body 201, a plug 202, an automatic quantitative blood collection tube 203, a sample adding tube 204 and a sealing sleeve 205; the tube body 201 is open at one end along the axial direction, and the plug 202 is detachably mounted at one end of the tube body 201 to form a sealing effect; the automatic quantitative blood collection tube 203 and the sample adding tube 204 are respectively fixed at both ends of the plug 202 along the axial direction, and the automatic quantitative blood collection tube 203 is located in the tube body 201; the automatic quantitative blood collection tube 203 is connected to the sample adding tube 204; and the sealing sleeve 205 is mounted at one end of the sample adding tube 204.

[0110] like Figure 4 and Figure 5 As shown, the blood cell analyzer also includes a blood cell detector 300 (whole denoted as 300), which includes a shell 301, an image sensor 302, a wireless transmitter 303 and an upper cover 304; the upper surface of the shell 301 is provided with a concave sample table 305 for placing the amplifying counting plate 101; the sample table 305 is provided with a light-transmitting hole 306 that matches the conical through hole 1014 on the amplifying counting plate 101; the image sensor 302 and the wireless transmitter 303 are located in a cavity enclosed by the shell 301, and the image sensor 302 is electrically connected to the wireless transmitter 303; the image sensor 302 is matched with the light-transmitting hole 306; the upper cover 304 is located on the upper surface of the shell 301, and a light source 307 is installed on the side of the upper cover 304 facing the shell.

[0111] like Figure 6 As shown, the blood cell analyzer also includes a data analyzer 400, which includes a data receiver, a data processor and a display screen 401; the data receiver receives the data signal in the wireless transmitter; the data receiver is electrically connected to the data processor; and the data processor is electrically connected to the display screen 401.

[0112] Example 2

[0113] Fig. 2(b-1) is a side view of the enlarged counting plate in this embodiment, and Fig. 2(b-2) is a top view of the enlarged counting plate in this embodiment.

[0114] The present embodiment provides another structure of the magnifying counting plate 101, as shown in FIG. 2(b-1) and FIG. 2(b-2), a groove 1013 is provided on the main plate 1011 of the magnifying counting plate 101, and an integrated plano-convex lens 1012 protruding outward is provided on the bottom surface of the main plate 1011 corresponding to the central area of ​​the groove 1013.

[0115] In this embodiment, the thickness of the plano-convex lens along the vertical direction is 0.3 mm, the diameter range of the plano-convex lens is 0.5-1.0 mm, and the thickness of the main body plate is 1.5 mm.

[0116] The following describes the method for using the blood cell analyzer provided in this embodiment.

[0117] First, blood is collected using the blood collection device 200. The specific operation is as follows: use a commercial blood collection needle to pierce the ring finger that has been disinfected with alcohol in advance, wipe off the first drop of blood, gently squeeze the blood from the finger, unscrew the seal 202 on the tube body 201 of the blood collection device 200, use an automatic quantitative blood collection tube 203 to absorb the blood, and then put it into the reagent tube 2 with the metal sealing film 1 torn off, as shown in Figure 3(b). The specifications of the reagent tube 2 are the same as those of the tube body 201 of the blood collection device 200. Cover the seal 202 tightly, as shown in Figure 3(c), and gently invert the reagent tube 2 to mix.

[0118] Then, the mixed blood sample (i.e., blood cell sample) is transferred into the sample processing plate 102. The specific operation is as follows: remove the sealing sleeve 205 on the sample tube 204, then invert the reagent tube 2, squeeze the reagent tube 2, discard the front end diluted blood in the sample tube 204, and then drop a drop of the subsequent diluted blood into the sample pool 1022 of the sample processing plate 102 until the gap under the cover glass 1024 is filled with the diluted blood sample.

[0119] Finally, the blood sample is tested and analyzed. The specific operation is: put the sample processing plate 102 into the groove 1013 in the magnifying counting plate 101, then put the magnifying counting plate 101 into the sample table 305 in the blood cell detector 300, close the upper cover 304 of the blood cell detector 300, turn on the power switch of the blood cell detector 300, and turn on the power switch of the data analyzer 400 at the same time, confirm that the blood cell detector 300 and the data analyzer 400 have been connected wirelessly, and the image of the blood cells will be displayed on the display screen 401. The data analyzer 400 automatically analyzes the cell number, morphology and white blood cell classification results through the built-in algorithm analysis software. The results are automatically stored in the data analyzer 400, and can also be transmitted to a specific data terminal or printed out as a report via Wifi. The analysis results are uploaded to the medical Internet cloud platform in real time.

[0120] The above are only a few embodiments of the present application, and are not intended to limit the present application in any form. Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application. Any technician familiar with the profession, without departing from the scope of the technical solution of the present application, using the technical content disclosed above to make slight changes or modifications are equivalent to equivalent implementation cases and fall within the scope of the technical solution.

Claims

1. A blood cell analyzer, characterized in that: The blood cell analyzer comprises a sample processing plate, an amplification counting plate and a blood cell detector; The sample processing plate is used to hold blood cell samples; The magnifying counting plate comprises a lens; The blood cell detector includes an image sensor; The sample processing plate is detachably mounted on the amplifying and counting plate, and the amplifying and counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample magnified by the lens is transmitted to the image sensor; The amplification counting plate also includes a main body plate; The main body plate is provided with a groove for fixing the sample processing plate; The lens is fixed below the groove through the main body plate; The main body plate is provided with a conical through hole in the vertical direction and connected with the groove, the lens is a biconvex spherical lens, the biconvex spherical lens is fixed in the through hole, and the through hole gradually decreases in diameter in the vertical direction; The diameter of the large diameter end of the through hole is 3 to 8 mm; the diameter of the small diameter end of the through hole is 0.3 to 0.8 mm; the height of the through hole in the axial direction is 1 to 5 mm; The diameter of the biconvex spherical lens ranges from 0.5 to 6 mm; The blood cell detector also includes a housing, an upper cover plate and a wireless transmitter; The upper surface of the housing is provided with a concave sample stage for placing the amplifying counting plate; The sample stage is provided with a light-transmitting hole matched with the lens in the magnifying counting plate; The image sensor and the wireless transmitter are located in a cavity enclosed by the shell, and the image sensor is electrically connected to the wireless transmitter; The image sensor is adapted to the light-transmitting hole; The upper cover plate is located on the upper surface of the shell, and a light source is installed on a surface of the upper cover plate facing the shell.

2. The blood cell analyzer according to claim 1, characterized in that: The sample processing plate comprises a glass slide, a support member and a cover glass; A concave sample pool is provided on the glass slide; The cover glass and the support are both located in the sample pool, and a gap for containing the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the support.

3. The blood cell analyzer according to claim 2, characterized in that: The height of the gap along the vertical direction is 0.05-0.12 mm.

4. The blood cell analyzer according to claim 1, characterized in that: The lens is a plano-convex lens, the plane end of the plano-convex lens is fixed on the outer bottom surface of the main body plate, and the convex end of the plano-convex lens is close to the cell detector.

5. The blood cell analyzer according to claim 4, characterized in that: The thickness of the plano-convex lens along the vertical direction is 0.05-2 mm, and the diameter range of the plano-convex lens is 0.3-6 mm; the thickness of the main body plate is 1-5 mm.

6. The blood cell analyzer according to claim 1, characterized in that: The blood cell analyzer also includes a blood collector, which includes a tube body, a plug, an automatic quantitative blood collection tube, a sample adding tube and a sealing sleeve; One end of the tube body along the axial direction is open, and the sealing plug is detachably mounted on the open end of the tube body; The automatic quantitative blood collection tube and the sample adding tube are respectively fixed on both sides of the plug along the axial direction, and the automatic quantitative blood collection tube is located in the tube body; The automatic quantitative blood collection tube is connected to the sample adding tube; The sealing sleeve is installed at one end of the sample adding tube.

7. The blood cell analyzer according to claim 1, characterized in that: The blood cell analyzer also includes a data analyzer, which includes a data receiver, a data processor and a display screen; The data receiver receives the data signal from the wireless transmitter; The data receiver is electrically connected to the data processor; The data processor is electrically connected to the display screen.

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