Device for studying magnetobiological responses to insect locomotor activity rhythms and sleep behavior

Abstract

The present invention discloses a device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior, comprising: an artificial magnetic field processing system for providing magnetic field processing for the entire device; an environmental factor control and monitoring system for providing a local light-transmitting space and capable of real-time monitoring of the environmental factors of the space, and located within the artificial magnetic field processing system; a test insect flight locomotor activity rhythm measurement system for locating test insects and measuring the test insect flight locomotor activity rhythm, and located within the environmental factor control and monitoring system; a test insect sleep behavior research system for monitoring the sleep behavior of the test insects located by the test insect flight locomotor activity rhythm measurement system, and located within the environmental factor control and monitoring system; an open source light source control system for providing the required light source for the environmental factor control and monitoring system from the outside; and a control and data storage system for controlling the entire device and collecting and analyzing data. The device of the present invention has precise control, high integration, and strong adaptability.

Description

Technical Field

[0001] The invention relates to the cross-research field of magnetobiology and chronobiology, and in particular to a magnetobiological response research device for insect locomotor activity rhythm and sleep behavior. Background Art

[0002] The Earth's magnetic field has a significant impact on biological activity. Through long-term coevolution with Earth's environment, many animals have evolved the ability to sense and utilize the Earth's magnetic field. While the mechanisms by which animals (including insects) perceive magnetic fields have been explored for over a century, the question of how they acquire and utilize the vectorial information in the Earth's magnetic field remains unresolved. With the rapid development of interdisciplinary research, researchers have used artificial magnetic fields to uncover a range of magnetobiological phenotypes in animals, including physiological, metabolic, and behavioral responses. These findings suggest that many animals possess highly conserved magnetoreception abilities, making it particularly important to investigate the mechanisms of animal magnetoreception through bio-magnetic field interactions. Currently, indoor insect magnetobiology research primarily utilizes Helmholtz coils to artificially manipulate the vectorial information of the Earth's magnetic field, which limits processing space. However, insects are considered excellent models for studying animal magnetoreception due to their small size, short generation cycle, ease of artificial breeding, and efficient molecular biology applications. Therefore, developing insect behavioral research devices suitable for magnetic field manipulation is crucial for furthering the understanding of animal magnetoreception mechanisms.

[0003] The circadian clock is an invisible "clock" within organisms. Studies have reported periodic circadian clocks at various timescales (e.g., near-circadian, seasonal, and annual), demonstrating that circadian clocks play a crucial role in biological life. Insects offer significant advantages for studying their temporal control mechanisms based on chronobiology, given their short generation cycles and ease of manipulation indoors. Selecting insects with rhythmic physiological and behavioral phenotypes (e.g., locomotor activity rhythms, phototropism rhythms, oviposition rhythms, eclosion rhythms, and flight rhythms) significantly facilitates the study of their intrinsic circadian clock mechanisms. This research will also help uncover the diverse mechanisms of circadian clock regulation associated with different phenotypes and their evolutionary significance. This research will also provide valuable insights into the application of chronobiology in fields such as medicine (e.g., circadian-based therapeutic strategies) and agriculture (e.g., early warning and forecasting of migratory pest damage in agriculture and the establishment of optimal timing strategies for efficient pesticide application).

[0004] From invertebrates to humans, sleep behavior is crucial for animals, such as maintaining physiological and neural homeostasis, cognitive and immune functions, cellular metabolism and communication between the brain and its controlled peripheral organs, learning and memory, etc. However, the neural and molecular basis of sleep regulation and how sleep is affected by the internal and external environment of animals are still unclear. Drosophila melanogaster), researchers have identified genes and neural circuits that regulate sleep. This study also revealed the potential conservation of sleep regulation mechanisms from fruit flies to mammals. In addition, studies have revealed the potential mechanisms for the integration of sleep and many different life processes, including circadian timing, metabolism, and aging. These findings indicate that sleep is composed of multiple physiological and behavioral states. At present, there are still many gaps in our analysis of sleep mechanisms, such as how sleep regulation interacts with migration regulation during the migration of migratory insects. Using insects as model organisms to conduct sleep behavior research is expected to promote the revelation of major scientific issues such as the basic biological significance of sleep and the role of sleep in human health and disease. The currently more widely used sleep behavior research device is mainly composed of a combination of a spontaneous activity monitoring device and a mechanical vibration deprivation sleep device based on fruit flies. The research subjects among insects are also mainly fruit flies. This device is less adaptable to larger insects such as Lepidoptera.

[0005] Both motor activity rhythms (such as flight rhythms) and sleep behavior are regulated by the circadian clock and serve as important phenotypes for studying chronobiology. Against this backdrop, current research on the intersection of chronobiology and magnetobiology, focusing on insects, holds significant promise for expanding animal chronobiological mechanisms, uncovering magnetoreception mechanisms, and even exploring magnetobiological effects in astrobiology. Therefore, the development of an experimental setup suitable for studying insect locomotor activity and sleep behavior under magnetic field conditions is urgently needed. Summary of the Invention

[0006] The purpose of the present invention is to address the problems existing in the prior art and provide a device for studying the magnetobiological response of insect locomotor activity rhythms and sleep behaviors. The device fills the gap in devices for studying the locomotor activity rhythms and sleep behaviors of insects after magnetic field treatment, and provides an integrated research system with scientific design, high efficiency, stability, open source, precise control of environmental factors, and accurate and reliable data acquisition for conducting research on the interaction between the biological clock and magnetoreception (magnetic response) mechanism of insects (such as fall armyworm, North American monarch butterfly, rice planthopper, trichogrammatid, sticky insect, locust, etc.) as research objects.

[0007] The purpose of the present invention is to be solved by the following technical solutions:

[0008] A device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior, characterized in that the device comprises:

[0009] An artificial magnetic field processing system, used to provide magnetic field processing for the entire device;

[0010] An environmental factor control and monitoring system, which is used to provide a local light-transmitting space and monitor the environmental factors of the space in real time, and is located within the artificial magnetic field processing system;

[0011] A test insect flight locomotor activity rhythm measurement system is used to locate the test insects and measure their flight locomotor activity rhythm, and is located within the environmental factor control and monitoring system;

[0012] The test insect sleep behavior research system is used to monitor the sleep behavior of test insects located in the test insect flight movement activity rhythm measurement system and is located within the environmental factor control monitoring system;

[0013] Open source light source control system, used to provide the required light source for the environmental factor control and monitoring system from the outside;

[0014] The control and data storage system is used to control and collect and analyze data from the artificial magnetic field processing system, environmental factor control and monitoring system, test insect flight movement activity rhythm measurement system, test insect sleep behavior research system, and open source light source control system.

[0015] The artificial magnetic field processing system includes a three-dimensional Helmholtz coil and a high-power programmable DC / AC power supply for adjusting the magnitude and direction of the current inside the three-dimensional Helmholtz coil; and the high-power programmable DC / AC power supply can communicate with the fluxgate meter in the environmental factor control and monitoring system to obtain the magnetic field strength in the environmental factor control and monitoring system in real time and adjust it according to requirements.

[0016] The environmental factor control and monitoring system includes an opaque white acrylic box located in the artificial magnetic field processing system. Temperature, humidity and light intensity sensors and a fluxgate meter are arranged on the inner wall of the opaque white acrylic box. The temperature, humidity and light intensity sensors are used to measure the temperature, humidity and light intensity in the opaque white acrylic box and feed back to the control and data storage system. The fluxgate meter is used to measure the magnetic field strength in the opaque white acrylic box and feed back to the control and data storage system.

[0017] The temperature, humidity and light intensity sensors are arranged along the inner wall of the opaque white acrylic box around the test insect; the fluxgate meter is located above the temperature, humidity and light intensity sensors.

[0018] The test insect flight locomotor activity rhythm measurement system includes an optical encoder connected to a control and data storage system. A rotating shaft is provided at the bottom of the optical encoder, and a demagnetizing alloy crossbar is installed at the lower end of the rotating shaft. One end of the demagnetizing alloy crossbar is used to fix a detachable tungsten rod hanging arm with a test insect, and the other end is used to fix a counterweight copper ball with the same weight as the test insect and the detachable tungsten rod hanging arm. The optical encoder is arranged on the top of an opaque white acrylic box in the environmental factor control and monitoring system.

[0019] The test insect sleep behavior research system includes a fisheye camera, a latex balloon for depriving the test insects of sleep, and a silent fan; the silent fan is located at the bottom of an opaque white acrylic box in the environmental factor control and monitoring system and can blow the latex balloon upward to deprive the test insects of sleep; the fisheye camera is located at the top of the opaque white acrylic box in the environmental factor control and monitoring system and is connected to a video storage device outside the artificial magnetic field processing system via a data line, and the video storage device is connected to a control and data storage system via a line.

[0020] The open source light source control system includes a light source and a light source setting processor, or a light source and a light source setting processor and a configured light homogenizing mechanism, wherein the light homogenizing mechanism is located at the top light-transmitting part of the opaque white acrylic box in the environmental factor control and monitoring system; the light source that provides the required illumination for the environmental factor control and monitoring system is located directly above the light homogenizing mechanism, and the light source is connected to the light source setting processor via a line, and the light source setting processor is connected to the control and data storage system via a line; the light source is a full-spectrum or optional-spectrum light source, and the light homogenizing mechanism is a light homogenizing plate, or a combination of a light homogenizing plate and a filter.

[0021] The control and data storage system includes a microcontroller for performing data processing and analysis and programming control of the automatic operation of the entire device, as well as a data storage control center. The microcontroller connected to the data storage control center is connected to the light source setting processor in the open source light source control system and the optical encoder in the test insect flight movement activity rhythm measurement system through lines, and can receive real-time data from the temperature, humidity and light intensity sensors and fluxgate meter in the environmental factor control and monitoring system; the data storage control center is connected to the video storage in the test insect sleep behavior research system through lines.

[0022] The control and data storage system is connected to the movable workstation via a line to achieve operation and data visualization.

[0023] The artificial magnetic field processing system and the environmental factor control and monitoring system are placed on a base, and a base bracket is provided at the bottom of the base.

[0024] The present invention has the following advantages over the prior art:

[0025] The artificial magnetic field processing system of the present invention can set an artificial magnetic field with a wider range of magnetic field strength and adjustable frequency through a high-power programmable DC / AC power supply; the test insect flight movement activity rhythm measurement system adopts a detachable tungsten rod boom, which can realize the test insects to be fixed once and then disassembled and fed daily for long-term measurement. On this basis, a highly integrated test insect sleep behavior research system is provided. In addition to conducting flight rhythm and flight ability research, it can also carry out sleep behavior research (such as sleep intensity and sleep rhythm) after treatment such as sleep deprivation; the control and data storage system adopts an optical encoder to communicate with the microcontroller, and the microcontroller programming is used to realize highly automated feedback operation of the device, which can greatly improve The objective accuracy and visualization effect of the data are obtained; the environmental factor control and monitoring system ensures the accuracy of environmental factor control during the experiment and the integrity of scientific research support data; the open source light source control system can adapt to full-spectrum and fixed-range wavelength light sources, and has the function of programming light cycles and lighting operation modes (such as simulating gradual changes in sunlight intensity, stepped light intensity, fixed light intensity, pulsed light, etc.); the device has a complete structure, scientific design and simple operation. It is an efficient and stable device that can adapt to a wide range of research objects, accurately control environmental factors, obtain accurate and objective data, and has highly visualized operation and data for conducting research on the magnetobiological response of insect locomotor activity rhythms and sleep behaviors.

[0026] The device of the present invention combines an artificial magnetic field processing system, a test insect flight movement activity rhythm measurement system, a test insect sleep behavior research system, an environmental factor control and monitoring system, an open source light source control system, and a control and data storage system. It has the advantages of precise parameter control, high integration, and high adaptability to research objects. The research and development of this device will further promote the progress of interdisciplinary research in magnetobiology and chronobiology. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Attachment Figure 1 This is a schematic diagram of the structure of the device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior according to the present invention;

[0028] Attachment Figure 2 Schematic diagram of the structure of the test insect flight movement activity rhythm measurement system of the present invention;

[0029] Attachment Figure 3 Schematic diagram of the structure of the artificial magnetic field processing system of the present invention.

[0030] Among them: 1—light source; 2—three-dimensional Helmholtz coil; 3—light homogenization mechanism; 4—fluxgate meter; 5—temperature, humidity and light intensity sensor; 6—weighted copper ball; 7—latex balloon; 8—base; 9—base bracket; 10—silent fan; 11—light source setting processor; 12—optical encoder; 13—rotating shaft; 14—fisheye camera; 15—demagnetizing alloy crossbar; 16—detachable tungsten rod boom; 17—opaque white acrylic box; 18—video storage; 19—microcontroller; 20—data storage control center; 21—mobile workstation; 22—high-power programmable DC / AC power supply. DETAILED DESCRIPTION

[0031] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0032] like Figure 1-3 As shown: A device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior, comprising an artificial magnetic field processing system, a test insect flight locomotor activity rhythm measurement system, a test insect sleep behavior research system, an environmental factor control and monitoring system, an open source light source control system, and a control and data storage system. The artificial magnetic field processing system and the environmental factor control and monitoring system are mounted on a base 8 having a base bracket 9. The system components include a light source 1, a three-dimensional Helmholtz coil 2, a light homogenizing mechanism 3, a fluxgate meter 4, a temperature, humidity and light intensity sensor 5, a counterweight copper ball 6, a latex balloon 7, a silent fan 10, a light source setting processor 11, an optical encoder 12, a rotating shaft 13, a fisheye camera 14, a demagnetizing alloy crossbar 15, a detachable tungsten rod boom 16, an opaque white acrylic box 17, a video memory 18, a microcontroller 19, a data storage control center 20, and a high-power programmable DC / AC power supply 22. Specifically: the artificial magnetic field processing system includes a three-dimensional Helmholtz coil 2, a high-power programmable DC / AC power supply 22, the environmental factor control and monitoring system includes a fluxgate meter 4, a temperature, humidity and light intensity sensor 5, and an opaque white acrylic box 17, the test insect flight movement activity rhythm measurement system includes an optical encoder 12, a counterweight copper ball 6, a rotating shaft 13, a demagnetizing alloy crossbar 15, and a detachable tungsten rod boom 16, the test insect sleep behavior research system includes a latex balloon 7, a silent fan 10, a fisheye camera 14, and a video storage device 18, the open source light source control system includes a light source 1, a uniform light mechanism 3, a light source setting processor 11, a control and data storage system microcontroller 19 and a data storage control center 20, and the data storage control center 20 can be connected to a movable workstation 21.

[0033] like Figure 2As shown, the optical encoder 12 and the rotating shaft 13 are embedded and fixed on the top of the opaque white acrylic box 17. The optical encoder 12 is connected to the rotating shaft 13 to obtain the flight angle, speed, direction, time and other information of the test insect through the supporting software; the demagnetization alloy cross bar 15 is connected to the bottom of the rotating shaft 13, and the two ends of the demagnetization alloy cross bar 15 are respectively a counterweight copper ball 6 and a detachable tungsten rod hanging arm 16. The end of the detachable tungsten rod hanging arm 16 is glued to the insect to be tested by hot melt glue. The size of the counterweight copper ball 6 is selected to be consistent with the total weight of the detachable tungsten rod hanging arm 16 and the insect to be tested. After the demagnetization alloy cross bar 15 maintains horizontal balance, data acquisition can begin.

[0034] like Figure 3 As shown, an opaque white acrylic box 17 is placed inside the three-dimensional Helmholtz coil 2, and the test insect fixed position area is located in the effective area of ​​the uniform magnetic field in the center of the three-dimensional Helmholtz coil 2. The three-dimensional Helmholtz coil 2 is connected to a high-power programmable DC / AC power supply 22. After power is turned on, the current size, direction, frequency, etc. are adjusted to generate an artificial magnetic field of the required intensity, direction, and frequency.

[0035] like Figure 1 As shown, the fisheye camera 14 is located on the top of the opaque white acrylic box 17 to monitor the sleep state of the insect to be tested in real time. The fisheye camera 14 is connected to the video memory 18, and the video memory 18 is connected to the movable workstation 21 through the data storage control center 20 to receive the monitoring image in real time; the silent fan 10 is located at the bottom of the opaque white acrylic box 17 and is equipped with a timer switch. The latex balloon 7 is above the silent fan 10, and the timer switch is set to randomly turn on and off at a time interval of 2-4 minutes. When the silent fan 10 is powered on, the latex balloon 7 will be blown upwards, and the sleep of the insect to be tested will be disturbed and deprived, so as to carry out subsequent sleep behavior research (such as sleep intensity, sleep rhythm, etc.) after sleep deprivation.

[0036] like Figure 1 As shown, the temperature, humidity and light intensity sensor 5 is located on the side wall of the opaque white acrylic box 17, which can record the temperature, humidity and light intensity data in real time and feed it back to the data and storage control center 20. It can also monitor the light intensity in real time, thereby communicating with the light source setting processor 11 and automatically adjusting the light intensity and appropriate height of the full spectrum or optional spectrum light source 1 according to the settings.

[0037] like Figure 1As shown, the full-spectrum or optional spectrum light source 1 is directly above the light homogenizing mechanism 3. The specific spectrum light source can be realized by the filter function combination of the light source 1 and the light homogenizing mechanism 3 (a light homogenizing plate, or a combination of a light homogenizing plate and an optional filter) or the optional spectrum light source in the optional spectrum light source 1 (such as a high-energy LED lamp, etc.). The light source frequency, light cycle, and light intensity of the full-spectrum or optional spectrum light source 1 can be controlled by the light source setting processor 11. The light source setting processor 11 has the function of programmable setting of the light cycle and the lighting operation mode. The light source can be set according to the experimental purpose to realize automated lighting program processing.

[0038] like Figure 1 As shown, the microcontroller 19 and the data storage control center 20 are connected in the control and data storage system. In addition to directly connecting and communicating with the fluxgate meter 4, the temperature, humidity and light intensity sensor 5, the light source setting processor 11 and the optical encoder 12, the microcontroller 19 also converts the optical signal formed by the optical encoder 12 into data through an independent program and transmits the result to the data storage control center 20. The synchronous communication ensures the consistency and accuracy of the test data; the data storage control center 20 is connected to the remote movable workstation 21 through a data line to realize the visualization of data and operations.

[0039] The following further illustrates the device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior provided by the present invention through specific examples 1 and 2.

[0040] Example 1 A study on the flight rhythm phenotype of Lepidoptera insects treated with a magnetic field was conducted.

[0041] like Figure 1-3 Shown is: a magnetobiological response research device for insect locomotor activity rhythm and sleep behavior, including an artificial magnetic field processing system, a test insect flight locomotor activity rhythm measurement system, a test insect sleep behavior research system, an environmental factor control and monitoring system, an open source light source control system, and a control and data storage system.

[0042] In the insect flight locomotor activity rhythm measurement system of this embodiment, the rotating shaft 13 is sleeved on the optical encoder 12, the demagnetizing alloy crossbar 15 is 16 cm long (rotation radius 8 cm), the detachable tungsten rod suspension arm 16 is 0.5 mm in diameter and 5 cm in length, and the mass of the homemade counterweight copper ball 6 is equal to the total weight of the detachable tungsten rod suspension arm 16 and the insect to be tested; the lepidopteran insect to be tested is an indoor population. A newly emerged lepidopteran insect is placed in a plastic cup and fed with 10% honey water. The test is started on the next day during the light period. The pubic hairs of the mesothorax of the insect to be tested are removed with ultraviolet quick-drying glue, and the insect is glued to the end of the detachable tungsten rod suspension arm 16 with hot melt glue so that the head and thorax axis of the insect to be tested is perpendicular to the demagnetizing alloy crossbar 15, and the light homogenizing mechanism 3 is placed on the top light-transmitting port of the opaque white acrylic box 17.

[0043] The environmental factor control and monitoring system of this embodiment monitors the constant temperature of the microenvironment according to the room temperature, which is about 26°C and the relative humidity is about 70%. The temperature, humidity and light intensity sensor 5 in the opaque white acrylic box 17 can feed back the data in real time to the microcontroller 19 and the data storage control center 20.

[0044] In the artificial magnetic field processing system of this embodiment, the three-dimensional Helmholtz coil 2 is connected via a high-power programmable DC / AC power supply 22 and connected via a data storage control center 20 to a mobile workstation 21 for visual software to control the magnitude, direction, and frequency of the DC current and thereby set the parameters of the artificial magnetic field generated by the three-dimensional Helmholtz coil 2. In this embodiment, the magnetic field processing group is set to a near-zero magnetic field of approximately 0 μT, and the control group is set to a local geomagnetic field of approximately 50 μT.

[0045] In the open source light source control system of this embodiment, a full-spectrum or optional-spectrum light source 1 is suspended above a light homogenizing mechanism 3. The frequency, light cycle, and light intensity of the full-spectrum or optional-spectrum light source 1 can be communicated and regulated by a light source setting processor 11 and a microcontroller 19. The experimental light cycle of the insects to be tested is set to LD = 15:9 (8:00 AM is the start time of light treatment).

[0046] In the control and data storage system of this embodiment, the optical encoder 12 communicates with the microcontroller 19, and the relevant parameters of the optical encoder 12 and the radius of the demagnetizing alloy crossbar 15 are set on the microcontroller 19. The acquired data is then transmitted to the microcontroller 19 for further program analysis and recording. This embodiment mainly records parameters such as activity rhythm (flight rhythm), activity intensity (flight distance), and finally transmits them to the data storage control center 20.

[0047] Example 2 Analysis of sleep behavior based on the flight rhythm phenotype of Lepidoptera insects.

[0048] like Figure 1-3 Shown is: a magnetobiological response research device for insect locomotor activity rhythm and sleep behavior, including an artificial magnetic field processing system, a test insect flight locomotor activity rhythm measurement system, a test insect sleep behavior research system, an environmental factor control and monitoring system, an open source light source control system, and a control and data storage system.

[0049] In the insect flight locomotor activity rhythm measurement system of this embodiment, the rotating shaft 13 is sleeved on the optical encoder 12, the demagnetizing alloy crossbar 15 is 16 cm long (rotation radius 8 cm), the detachable tungsten rod suspension arm 16 is 0.5 mm in diameter and 5 cm in length, and the mass of the homemade counterweight copper ball 6 is equal to the total weight of the detachable tungsten rod suspension arm 16 and the insect to be tested; the lepidopteran insect to be tested is an indoor population. A newly emerged lepidopteran insect is placed in a plastic cup and fed with 10% honey water. The test is started on the next day during the light period. The pubescent hairs of the mesothorax of the insect to be tested are removed with ultraviolet quick-drying glue, and the insect is glued to the end of the detachable tungsten rod suspension arm 16 with hot melt glue so that the head and thorax axis of the insect to be tested is perpendicular to the demagnetizing alloy crossbar 15, and the light homogenizing mechanism 3 is placed on an opaque white acrylic box 17.

[0050] In the insect sleep behavior research system of this embodiment, a 9-hour dark period (sleep deprivation) was set for the treatment group. Specifically, during the dark period, a silent fan 10 was randomly activated at 2-4 minute intervals using an electronic timer, and a latex balloon 7 was blown to continuously disturb the insects for 30 seconds, depriving them of sleep. (Most sleep behavior research systems consider insects to be inactive for 5 minutes or longer to enter a sleep state.) After the dark period, the silent fan 10 stopped. A fisheye camera 14, mounted atop an opaque white acrylic enclosure 17, monitored the actual insect movements within the apparatus. This was monitored and reviewed in real time on a remotely connected mobile workstation 21, eliminating invalid samples where the insects flapped their wings but failed to rotate the shaft 13.

[0051] In the artificial magnetic field treatment system of this embodiment, a three-dimensional Helmholtz coil 2 is connected via a high-power programmable DC / AC power supply 22, and the visualization software of a mobile workstation 21 is connected via a microcontroller 19 to control the magnitude, direction, and frequency of the DC current, thereby setting the parameters of the artificial magnetic field generated by the three-dimensional Helmholtz coil 2. In this embodiment, the magnetic field treatment group is set to the geomagnetic field of the test insect migration path of about 45 μT, and the control group is set to the local geomagnetic field of about 50 μT.

[0052] The environmental factor control and monitoring system of this embodiment monitors the constant temperature of the microenvironment to be approximately 26°C and the relative humidity to be approximately 70% based on the room temperature; the temperature, humidity and light intensity sensor 5 in the opaque white acrylic box 17 can feed back the data in real time to the data storage control center 20.

[0053] In the open-source light source control system of this embodiment, a full-spectrum or optional-spectrum light source 1 is suspended above a light homogenizing mechanism 3. The frequency, photoperiod, and light intensity of the full-spectrum or optional-spectrum light source 1 can be controlled and regulated via a light source setting processor 11 communicating with a microcontroller 19. The experimental photoperiod of the insects to be tested is set to LD = 15:9 (8:00 AM is the start time of light treatment).

[0054] In the control and data storage system of this embodiment, the locomotion activity rhythm (flight rhythm) data of insects after sleep deprivation recorded and stored in the data storage control center 20 of this system is used, and the parameters such as sleep rebound behavior, sleep rhythm, sleep intensity (being still for ≥5 minutes is considered as falling asleep and data statistics of sleep intensity and rhythm are performed), activity rhythm (flight rhythm), activity intensity (flight distance) after sleep deprivation are selected for subsequent personalized analysis.

[0055] The device of the present invention has a complete structure, scientific design and simple operation. It provides an integrated research system based on the intersection of biomagnetic response and chronobiology, which is efficient, stable, open source, highly adaptable to research objects and phenotypes, precisely controllable environmental factors, and accurately acquired data that can be efficiently transmitted and stored. It can be used in the study of the interaction between the biological clock (including motor activity rhythms and sleep behavior phenotypes) and magnetoreception (magnetic response) mechanism of insects (such as fall armyworm, North American monarch butterfly, rice planthopper, trichogrammatid, sticky insect, locust, etc.) as research objects.

[0056] The above embodiments are only for illustrating the technical ideas of the present invention and cannot be used to limit the scope of protection of the present invention. Any changes made on the basis of the technical solutions in accordance with the technical ideas proposed by the present invention fall within the scope of protection of the present invention; any technologies not involved in the present invention can be implemented by existing technologies.

Claims

1. A device for studying the magnetobiological response of insect locomotor activity rhythms and sleep behaviors, characterized by: The device includes: An artificial magnetic field processing system, used to provide magnetic field processing for the entire device; An environmental factor control and monitoring system is used to provide a local light-transmitting space and monitor the environmental factors of the space in real time, and is located within the artificial magnetic field processing system; A test insect flight locomotor activity rhythm measurement system is used to locate the test insects and measure their flight locomotor activity rhythm, and is located within the environmental factor control and monitoring system; The test insect sleep behavior research system is used to monitor the sleep behavior of test insects located in the test insect flight movement activity rhythm measurement system and is located within the environmental factor control monitoring system; Open source light source control system, used to provide the required light source for the environmental factor control and monitoring system from the outside; Control and data storage system, used to control and collect and analyze data from the artificial magnetic field processing system, environmental factor control and monitoring system, test insect flight movement activity rhythm measurement system, test insect sleep behavior research system, and open source light source control system; The test insect flight movement activity rhythm measurement system includes an optical encoder (12) connected to a control and data storage system, a rotating shaft (13) is provided at the bottom of the optical encoder (12), and a demagnetizing alloy crossbar (15) is installed at the lower end of the rotating shaft (13), one end of the demagnetizing alloy crossbar (15) is used to fix a detachable tungsten rod hanging arm (16) with a test insect, and the other end is used to fix a counterweight copper ball (6) with the same weight as the test insect and the detachable tungsten rod hanging arm (16); the optical encoder (12) is set on the top of an opaque white acrylic box (17) in the environmental factor control and monitoring system; The artificial magnetic field processing system and the environmental factor control and monitoring system are placed on the base (8); The test insect sleep behavior research system comprises a fisheye camera (14), a latex balloon (7) for depriving the test insect of sleep, and a silent fan (10); the silent fan (10) is located at the bottom of an opaque white acrylic box (17) in the environmental factor control and monitoring system and can blow the latex balloon (7) upward to deprive the test insect of sleep; the fisheye camera (14) is located at the top of the opaque white acrylic box (17) in the environmental factor control and monitoring system and is connected to a video memory (18) outside the artificial magnetic field processing system through a data line, and the video memory (18) is connected to a control and data storage system through a line.

2. The device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior according to claim 1, characterized in that: The artificial magnetic field processing system includes a three-dimensional Helmholtz coil (2) and a high-power programmable DC / AC power supply (22) for adjusting the magnitude and direction of the internal current of the three-dimensional Helmholtz coil (2); and the high-power programmable DC / AC power supply (22) can communicate with a fluxgate meter (4) in an environmental factor control and monitoring system to obtain the magnetic field strength in the environmental factor control and monitoring system in real time and adjust it according to requirements.

3. The device for studying insect locomotor activity rhythm and sleep behavior magnetobiological response according to claim 1, characterized in that: The environmental factor control and monitoring system includes an opaque white acrylic box (17) located in the artificial magnetic field processing system, and a temperature, humidity and light intensity sensor (5) and a fluxgate meter (4) are arranged on the inner wall of the opaque white acrylic box (17). The temperature, humidity and light intensity sensor (5) is used to measure the temperature, humidity and light intensity in the opaque white acrylic box (17) and feed back to the control and data storage system, and the fluxgate meter (4) is used to measure the magnetic field strength in the opaque white acrylic box (17) and feed back to the control and data storage system.

4. The device for studying insect locomotor activity rhythm and sleep behavior magnetobiological response according to claim 3, characterized in that: The temperature, humidity and light intensity sensor (5) is arranged along the inner wall of the light-proof white acrylic box (17) around the test insect; the fluxgate meter (4) is located above the temperature, humidity and light intensity sensor (5).

5. The device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior according to claim 1, characterized in that: The open source light source control system includes a light source (1) and a light source setting processor (11), or a light source (1) and a light source setting processor (11) and a configured light homogenizing mechanism (3), wherein the light homogenizing mechanism (3) is located at the top light-transmitting part of the opaque white acrylic box (17) in the environmental factor control and monitoring system; the light source (1) that provides the required light source for the environmental factor control and monitoring system is located directly above the light homogenizing mechanism (3), and the light source (1) is connected to the light source setting processor (11) through a line, and the light source setting processor (11) is connected to the data storage system through a line and a control; the light source (1) is a full-spectrum or optional-spectrum light source, and the light homogenizing mechanism (3) is a light homogenizing plate, or a combination of a light homogenizing plate and a filter.

6. The device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior according to claim 1, characterized in that: The control and data storage system includes a microcontroller (19) for performing data processing and analysis and programming control of the automatic operation of the entire device, and a data storage control center (20). The microcontroller (19) connected to the data storage control center (20) is connected to the light source setting processor (11) in the open source light source control system and the optical encoder (12) in the test insect flight movement activity rhythm measurement system through lines, and can receive real-time data from the temperature, humidity and light intensity sensor (5) and the fluxgate meter (4) in the environmental factor control and monitoring system; the data storage control center (20) is connected to the video storage (18) in the test insect sleep behavior research system through lines.

7. The device for studying the magnetobiological response of insect locomotor activity rhythm and sleep behavior according to claim 1 or 6, characterized in that: The control and data storage system is connected to the movable workstation (21) via a line to achieve operation and data visualization.

8. The device for studying insect locomotor activity rhythm and sleep behavior magnetobiological response according to claim 1, characterized in that: A base bracket (9) is provided at the bottom of the base (8).

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