A rocking mechanism within a protein blotter

Abstract

The application provides a swing mechanism in a protein blotter, which comprises a film bearing device, a connecting rod mechanism and a rotating mechanism, the rotating mechanism is a driving component in the swing mechanism and is used for providing kinetic energy for the swing mechanism; the rotating mechanism is connected with the connecting rod mechanism, and the rotating mechanism can drive the connecting rod mechanism to move; the connecting rod mechanism is connected with the film bearing device, and the rotating mechanism can drive the film bearing device to move through the connecting rod mechanism, so that the film bearing device swings repeatedly. The swing structure of the application has low requirements on the motor in the rotating mechanism, and the motor used in the application does not need a large rotating torque compared with the motor in the traditional protein blotter; meanwhile, the application does not need to control the motor complicatedly, such as accurately controlling the rotating speed of the motor and controlling the rotating direction.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a swing mechanism in a protein blotting instrument. Background Technology

[0002] Western blotting (also known as protein blotting) is a method for detecting a specific protein in a complex sample based on the specific binding of antigens and antibodies. It is a novel immunochemical technique developed from gel electrophoresis and solid-phase immunoassay. Due to the high resolution of gel electrophoresis and the high specificity and sensitivity of solid-phase immunoassay, Western blotting has become a routine technique for protein analysis. It is most commonly used for detecting protein characteristics, expression, and distribution, such as the detection of viral antibodies or antigens, the mass determination of peptide molecules, and the qualitative or semi-quantitative detection of tissue antigens.

[0003] Currently, Western blotting-related tests are typically performed on automated Western blotting instruments. These instruments separate the target proteins based on their properties, such as molecular weight, size, charge, and isoelectric point, using different electrophoretic methods. The proteins in the gel are then transferred to a polyvinylidene fluoride (PVDF) membrane using an electric current. The principle of specific binding between antibodies and antigens is utilized, with the antibody acting as a probe to capture the target protein. It is important to note that a non-specific protein, such as bovine serum albumin, should be added before adding the antibody to "block" the membrane and prevent non-specific binding of the antibody.

[0004] After the membrane and reagents are placed into the automated Western blot analyzer, the instrument's own swing mechanism reciprocates to ensure thorough fusion of the membrane and antigen, thereby obtaining accurate test results. However, traditional swing mechanisms suffer from problems such as high required torque, insufficient adaptability, and reagents easily spilling out of the automated Western blot analyzer. Therefore, a new swing structure needs to be designed to overcome these shortcomings. Summary of the Invention

[0005] The purpose of this invention is to provide a swing mechanism within a protein blot instrument to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the technical solution adopted by this invention is a swing mechanism within a Western blot instrument. This mechanism, used to reciprocate the swinging motion of membranes and reagents placed within the automated Western blot instrument, ensures thorough fusion and reaction between the membrane and antigen, thereby obtaining accurate test results. The swing mechanism includes a membrane support device, a linkage mechanism, and a rotating mechanism. The rotating mechanism is the driving component within the swing mechanism, typically controlled by a motor, providing kinetic energy to the swing mechanism. The rotating mechanism is connected to the linkage mechanism, enabling it to move. The linkage mechanism is connected to the membrane support device. All detection steps within the Western blot instrument, such as liquid addition, incubation, binding, sample addition, and washing, are performed within the membrane support device. The rotating mechanism, through the linkage mechanism, drives the membrane support device to move, achieving repeated swinging of the membrane support device. This reciprocating swinging of the membrane support device ensures thorough fusion and reaction between the sample and reagents, thereby achieving accurate detection.

[0007] Furthermore, the membrane support device has a pivot point around which it can rotate. Generally, to ensure uniform fusion of the membrane and antigen within the instrument, this pivot point is located on the vertical plane of the membrane support device. This pivot point is also located on a fixed plate, and the membrane support device and the fixed plate are rotatably connected via this pivot point. To ensure the installation and rotational stability of the membrane support device on the fixed plate, two pivot points are provided. As a preferred embodiment, the fixed plate has a fixed seat with two supporting ears. Each supporting ear has a through hole for mounting a pin. A support plate is fitted onto the pin, and the support plate is connected to the membrane support device. This pin serves as the pivot point and center of rotation for the membrane support device and the fixed seat.

[0008] Furthermore, to improve the smoothness of rotation at the pivot point, sliding components are provided between the pin and the two support ears, and between the pin and the support plate. The most common sliding component is a rolling bearing, which has advantages such as low cost and stable operation. However, it also has the problem of frequently needing to replenish lubricating oil or grease, which is cumbersome, especially in a protein blot instrument, where the pivot point of the membrane support device needs to rotate at high frequency, requiring regular maintenance by technicians, which is too cumbersome. In this embodiment, the sliding component uses a sliding bearing (press-fit bearing) made of special engineering plastic, such as the sliding bearing from German iglidur, which has a series of characteristics such as high wear resistance, dust resistance, dirt resistance, lubrication-free, and maintenance-free operation, making it suitable for this device.

[0009] Furthermore, the linkage mechanism includes a first connecting structure, a second connecting structure rotatably connected to the first connecting structure, and a third connecting structure rotatably connected to the second connecting structure. The first connecting structure is connected to the rotating mechanism, and the third connecting structure is connected to the membrane support device. The first, second, and third connecting structures can be three connecting rods, with adjacent connecting rods rotatably connected. The simplest rotatable connection method is to insert a rotating shaft into the rotatable position of adjacent connecting rods. During operation, the rotating shaft and the connected rods will rotate frequently. A similar technical solution to the aforementioned rotating fulcrum can be adopted, using sliding bearings made of special engineering plastics at the rotating parts. In this embodiment, since the first connecting structure is connected to the motor in the rotating mechanism, if the first connecting structure is a connecting rod, the motor shaft will be subjected to extremely uneven loads due to the gravity and centrifugal force of the first connecting structure when the motor is working. This will affect the service life of the motor. As a preferred technical solution, the first connecting structure is disc-shaped to distribute its gravity as evenly as possible, which can reduce the uneven load on the motor shaft. The disc-shaped first connecting structure and the second connecting structure rotatably connected thereon constitute a structure similar to an eccentric wheel.

[0010] Furthermore, the membrane support device is typically rectangular. To ensure uniform force distribution on the membrane support device when the motor in the rotating mechanism starts, the third connecting structure is located on the vertical plane along the long side of the membrane support device. It is important to note that the third connecting structure is eccentrically positioned; that is, it cannot be located on the line connecting the two rotational fulcrums. If the third connecting structure is located on this line, the rotating mechanism cannot drive the membrane support device to swing. Furthermore, all other things being equal, the greater the distance between the third connecting structure and the rotational fulcrum (i.e., the greater the eccentricity), the smaller the maximum swing amplitude of the membrane support device.

[0011] Furthermore, to facilitate the counting of the number of swaying events generated by the membrane support within the device, an optical coupler baffle is provided on the linkage mechanism. Correspondingly, an optical coupler sensing structure is also provided within the device. As the rotating mechanism starts and drives the linkage mechanism to move, the optical coupler baffle on the linkage mechanism passes through the optical coupler sensing structure once (blocking it once) for each revolution of the motor within the rotating mechanism, thus performing one count. Furthermore, the optical coupler baffle is installed on the second connecting structure. When the membrane support device is in a horizontal position, the right end of the optical coupler baffle precisely blocks the optical coupler sensor, which is located on the necessary path of the optical coupler baffle during its movement.

[0012] Furthermore, the motor within the rotating mechanism remains stationary to ensure that the amplitude, frequency, angle, and other parameters of each oscillation during membrane bearing manufacturing remain consistent. In this embodiment, the fixed plate is stationary, therefore the rotating mechanism can be mounted on the fixed plate. As a preferred technical solution, a first mounting plate is mounted on the fixed plate. The first mounting plate is L-shaped and can mount the motor, the output shaft of which is connected to the first connecting structure. A second mounting plate is provided on the first mounting plate, which is used to mount the optocoupler sensing structure.

[0013] Furthermore, when the membrane support is manufactured in a horizontal state, the rotating mechanism is located on the lower side of the third connecting structure.

[0014] Furthermore, the motor shaft within the rotating mechanism is located directly below the third connecting structure, meaning the line connecting the third connecting structure and the motor shaft is vertical.

[0015] In summary, the beneficial effects of this invention are:

[0016] The swing structure of this invention places very low demands on the motor within the rotating mechanism. Compared to the motors in traditional Western blot instruments, the motor used in this invention does not require a large torque. Furthermore, this invention eliminates the need for complex motor control, such as precise control of motor speed and rotation direction. Moreover, the motor used in this invention can be a conventional stepper motor, which can achieve precise steering and speed control of the swing structure through simple unidirectional rotation, significantly reducing the production cost of the Western blot instrument. Additionally, the swing structure features multiple speed adjustment levels, making it suitable for various working environments or meeting different work requirements. Finally, the rotating pairs in this invention all use sliding bearings made of special engineering plastics, which possess a series of characteristics such as high wear resistance, dust resistance, dirt resistance, lubrication-free operation, and maintenance-free operation. Technicians no longer need to perform regular maintenance, reducing maintenance costs and greatly extending the overall service life of the device. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of a rocking mechanism in a protein blot instrument according to the present invention in one direction;

[0019] Figure 2This is a schematic diagram of the swing mechanism in a protein blot instrument of the present invention in another direction;

[0020] Figure 3 This is an exploded view of the structure of the swing mechanism in a protein blot instrument of the present invention, located at the pivot point.

[0021] Figure 4 It is the rotational speed curve of part 1 given in the motion simulation;

[0022] Figure 5 This is the rotational speed curve obtained from part 3 in the motion simulation.

[0023] Figure 6 This is a schematic diagram of part 3 in a horizontal state during motion simulation.

[0024] Figure 7 This is a schematic diagram of part 3 being in a near-right limit state during motion simulation.

[0025] Figure 8 This is a schematic diagram showing part 3 in a near-horizontal state again during motion simulation.

[0026] Figure 9 This is a schematic diagram of part 3 in a motion simulation when it is in a state close to the left limit. Detailed Implementation

[0027] To make the technical problems to be solved by the present invention, the technical solutions and beneficial effects clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. It should be noted that the embodiments are only a detailed description of the present invention and should not be regarded as a limitation of the present invention. All features disclosed in the embodiments of the present invention, or all steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any way.

[0028] This embodiment provides a swing mechanism within a Western blot instrument. This mechanism, used to reciprocate the swinging motion of membranes and reagents placed within the automated Western blot instrument, ensures thorough fusion and reaction between the membrane and antigen, thereby obtaining accurate test results. The swing mechanism includes a membrane support device 10, a linkage mechanism 20, and a rotation mechanism 30. The rotation mechanism 30 is the driving component within the swing mechanism, typically controlled by a motor, providing kinetic energy to the swing mechanism. The rotation mechanism 30 is connected to the linkage mechanism 20, enabling the linkage mechanism 20 to move. The linkage mechanism 20 is connected to the membrane support device 10. All detection steps within the Western blot instrument, including liquid addition, incubation, binding, sample addition, and washing, are performed within the membrane support device 10. The rotation mechanism 30, through the linkage mechanism 20, drives the membrane support device 10 to move, achieving repeated swinging of the membrane support device 10. This reciprocating swinging of the membrane support device 10 ensures thorough fusion and reaction between the sample and reagent, thereby achieving accurate detection.

[0029] See attached document Figure 1 The membrane support device 10 has a pivot point 11, around which it can rotate. Generally, to ensure uniform fusion of the membrane and antigen within the instrument, the pivot point 11 is located on the perpendicular bisector (the perpendicular line in the figure, or the perpendicular plane in three-dimensional space) of the membrane support device 10. The pivot point 11 is also located on the fixed plate 12. The membrane support device 10 and the fixed plate 12 are rotatably connected via the pivot point 11. To ensure the installation and rotational stability of the membrane support device 10 on the fixed plate 12, two pivot points 11 are provided, as shown in the attached figure. Figure 2 As shown. Specifically, the fixing plate 12 is provided with a fixing seat 121, and the fixing seat 121 is provided with two supporting ears 122. Each of the two supporting ears 122 is provided with a through hole 123 for installing a pin 124. A support plate 125 is also sleeved on the pin 124, and the support plate 125 is connected to the membrane support device 10. The aforementioned pin 124 is the rotation fulcrum 11 of the membrane support device 10 and the fixing seat 121, and is also their rotation center.

[0030] See attached document Figure 1The linkage mechanism 20 includes a first connecting structure 21, a second connecting structure 22 rotatably connected to the first connecting structure 21, and a third connecting structure 24 rotatably connected to the second connecting structure 22. The first connecting structure 21 is connected to the rotating mechanism 30, and the third connecting structure 24 is connected to the membrane support device 10. The first connecting structure 21, the second connecting structure 22, and the third connecting structure 24 can be three connecting rods, with adjacent connecting rods rotatably connected. The simplest rotatable connection method is to insert a rotating shaft into the rotatable position of the adjacent connecting rods. In this embodiment, since the first connecting structure 21 is connected to the motor in the rotating mechanism 30, if the first connecting structure 21 is a connecting rod, the motor shaft will be subjected to extremely uneven loads due to the gravity and centrifugal force of the first connecting structure 21 when the motor is working. This will affect the service life of the motor. As a preferred technical solution, the first connecting structure 21 is disc-shaped to distribute its gravity as evenly as possible, which can reduce the uneven load on the motor shaft. The disc-shaped first connecting structure 21 and the second connecting structure 22 rotatably connected thereon constitute a structure similar to an eccentric wheel.

[0031] Preferably, the membrane support device 10 is typically rectangular. To ensure uniform force distribution on the membrane support device 10 when the motor within the rotating mechanism 30 is started, the third connecting structure 24 is located on the mid-vertical surface along the long side of the membrane support device 10, as shown in the attached figure. Figure 2 As shown in the attached diagram. It should be noted that the third connecting structure 24 is eccentrically positioned, as shown in the attached diagram. Figure 1 As shown, the third connecting structure 24 cannot be located on the line connecting the two rotation fulcrums 11, because when the third connecting structure 24 is located on the line connecting the two rotation fulcrums 11, the rotation mechanism 30 cannot drive the membrane support device 10 to perform a swaying motion. Furthermore, under the same conditions, the greater the distance between the third connecting structure 24 and the rotation fulcrum 11, that is, the greater the eccentricity, the smaller the maximum swaying amplitude of the membrane support device 10.

[0032] In conventional automated Western blot instruments, servo motors are used. The output shaft of the servo motor is connected to the rotation fulcrum 11 of the membrane support fabrication 10. To achieve the swinging motion, positive and negative pulse signals need to be input to the servo motor, which is cumbersome. Furthermore, servo motors are expensive, significantly increasing the cost of the Western blot instrument. In this invention, under the action of the linkage mechanism 20, the motor in the rotation mechanism 30 only needs to rotate in one direction to control the swinging operation of the membrane support fabrication 10. Repeated input of positive and negative pulse signals is no longer required, which is much more convenient. At the same time, without the need for repeated input of positive and negative pulse signals, the rotational accuracy requirement for the motor is eliminated. The servo motor can be replaced by a common stepper motor, which is far less expensive than a servo motor, significantly reducing the manufacturing cost of the automated Western blot instrument.

[0033] Preferably, to facilitate the counting of the number of swaying cycles of the inner membrane bearing manufacturing 10 in the device, a photocoupler baffle 14 is provided on the linkage mechanism 20. Correspondingly, a photocoupler sensing structure 15 is also provided inside the device. As the rotating mechanism 30 starts and drives the linkage mechanism 20 to move, for each revolution of the motor inside the rotating mechanism 30, the photocoupler baffle 14 on the linkage mechanism 20 passes through the photocoupler sensing structure 15 once (blocking it once), and a count is performed. Further, as shown in the attached... Figure 1 As shown, the optical coupler baffle 14 is installed on the second connection structure 22. When the membrane support device 10 is in a horizontal position, the right end of the optical coupler baffle 14 just blocks the optical coupler sensor 15. The optical coupler sensor 15 is on the necessary path of the optical coupler baffle 14 during its movement.

[0034] Preferably, the motor within the rotating mechanism 30 remains stationary to ensure that the amplitude, frequency, angle, and other parameters of the membrane bearing manufacturing 10 remain consistent with each oscillation. In this embodiment, the fixing plate 12 is stationary, therefore the rotating mechanism 30 can be mounted on the fixing plate 12. Specifically, a first mounting plate 16 is mounted on the fixing plate 12. The first mounting plate 16 is L-shaped and can mount the motor 17. The output shaft of the motor 17 is connected to the first connecting structure 21. A second mounting plate 18 is provided on the first mounting plate 16, and the second mounting plate 18 is used to mount the optocoupler sensing structure 15.

[0035] Preferably, when the membrane support manufacturing 10 is in a horizontal state, the rotating mechanism 30 is located below the third connecting structure 24. Further, the shaft of the motor 17 within the rotating mechanism 30 is located directly below the third connecting structure 24, meaning the line connecting the third connecting structure 24 and the shaft of the motor 17 is vertical.

[0036] Preferably, the present invention has at least four revolute joints, namely the revolute joint of the motor in the rotating mechanism 30, the revolute joint between the first connecting structure 21 and the second connecting structure 22, the revolute joint between the second connecting structure 22 and the third connecting structure 24, and the revolute joint at the position of the rotating fulcrum 11. These rotating pairs all require high-frequency rotation during the operation of the protein blot instrument. For example, at the rotation fulcrum 11, the membrane support assembly 10 will repeatedly oscillate around the rotation fulcrum 11. To improve the smoothness of rotation, sliding components 13 are provided between the pin 124 and the two support ears 122, and between the pin 124 and the support plate 125. The most common sliding component 13 is a rolling bearing, which has the advantages of low cost and stable operation. However, it also has the problem of frequently needing to add lubricating oil or grease, which is quite cumbersome. If lubricating oil or grease is not added in time, it may cause the bearing material to peel off, the bearing edge to scratch, and thus the roller to overheat. Extreme local heat will cause metal flow in the bearing, changing the original material and geometry of the bearing, ultimately causing the roller to tilt excessively, the cage to be damaged, and the bearing to lock completely. Therefore, in these high-frequency rotation environments, if rolling bearings are used, technicians need to check and maintain them regularly, which is very cumbersome.

[0037] In this embodiment, the sliding component 13 is a sliding bearing (press-fit bearing) made of special engineering plastic. A suitable thermoplastic base plastic material is selected, typically reinforced with reinforcing fibers to enhance compressive strength, and also contains solid lubricant to optimize wear resistance. These solid lubricant particles "embedded" in the matrix material are crucial for "dry operation." During operation, the sliding bearing releases thousands of solid lubricant particles stored in the matrix material due to pressure and movement, allowing them to reach the contact surface between the shaft and the bearing. These particles are sufficient to provide adequate solid lubrication to the contact surface, thus achieving dry operation. Sliding bearings made of engineering plastics possess a series of characteristics such as high wear resistance, dust resistance, dirt resistance, lubrication-free operation, and maintenance-free operation, making them particularly suitable for this device. Technicians no longer need to perform regular maintenance, reducing maintenance costs and significantly increasing the overall service life of the device. Specifically, sliding bearings from German brand iglidur can be used. Here, the rotating pair at the pivot point 11 is used as an example; similar structures can be used for rotating pairs at other locations.

[0038] In existing automated protein blotting instruments, the oscillation operation performed by the membrane support device 10 is controlled by a servo motor inputting positive and negative pulse signals. When the membrane support device 10 moves to its limit position to one side, a negative pulse signal needs to be immediately switched to move it to the other side to prevent excessive movement and reagent spillage. However, there is a sudden signal abrupt change during the switching of the two pulse signals. At this instant, the membrane support device 10 suddenly reverses direction, while the reagent inside retains its inertia and continues moving in the original direction. This collision can easily cause reagent spillage. To solve this problem, technicians typically reduce the input pulse signal as the membrane support device 10 approaches its limit position to make this abrupt change as gradual as possible. Once the membrane support device 10 has passed its limit position, a negative pulse signal is amplified to accelerate the reverse oscillation… This entire control process is extremely cumbersome and further increases the manufacturing cost of the device.

[0039] To highlight the advantages of the swing mechanism of this invention, motion simulation was performed on several core components within the swing mechanism, referring to the attached diagram. Figure 4 -Appendix Figure 9 Part 1 corresponds to the first connecting structure 21 mentioned above, part 2 corresponds to the second connecting structure 22 mentioned above, part 3 corresponds to the membrane support device 10 mentioned above, part 4 corresponds to the fixing plate 12, arrow 5 corresponds to the velocity (vector) at the edge position of the membrane support device 10, the length of arrow 5 corresponds to the magnitude of the velocity, and the direction of arrow 5 corresponds to the direction of the velocity.

[0040] Now, part 1 is given an angular velocity of 15 r / min, that is, 15 revolutions per minute. Figure 4 Input curve for the dynamic (angular velocity) of part 1; attached Figure 5 The output curve of the power (angular velocity) of part 4 around its fulcrum.

[0041] See attached document Figure 6 This state refers to the speed of movement of the edge of part 3 when it is in a horizontal position (arrow 5, the same below); see attached... Figure 7 This status is attached. Figure 6 After 1 second, when part 3 is in a state close to its right limit, the speed of its edge movement is as follows; refer to the attached diagram. Figure 8 This status is attached. Figure 7 After 1 second, when part 3 is in a nearly horizontal state, the speed of its edge movement is as follows; refer to the attached diagram. Figure 9 This status is attached. Figure 8 After 1 second, when part 3 is in a state close to the left limit, the speed of its edge movement.

[0042] Combined with appendix Figure 5 and appendix Figure 6-9 The diagrams for each state clearly show that when part 3 is in a horizontal state, refer to the attached diagram. Figure 6 and attached Figure 8 Because component 3 is placed stably, the reagents inside are not easily leaked. Therefore, it is desirable to give component 3 a higher rotation speed to help the membrane and antigen inside component 3 to fully fuse. In this invention, the attached... Figure 6 and attached Figure 8 The corresponding arrow 5 is longer, attached. Figure 5 The instantaneous velocity corresponding to this is close to the maximum rotational speed. When part 3 is in a horizontal state, the swing mechanism of this invention can just give part 3 a relatively large motion speed, that is, give part 3 a relatively large angular velocity at the rotation fulcrum position (rotation fulcrum 11 of the membrane support device 10); when part 3 is in the left or right extreme state, refer to the attached... Figure 7 and attached Figure 9 The reagent inside is more prone to leakage because part 3 is in an inclined state. Furthermore, in both of these states, part 3 immediately needs to reverse its oscillation, which further promotes reagent leakage. To avoid leakage, it is desirable to give part 3 a relatively low rotational speed to make this transformation process as smooth as possible. In this invention, the attached... Figure 7 and attached Figure 9 The corresponding arrow 5 is shorter, with... Figure 5 The instantaneous rotational speed is close to 0, which perfectly achieves deceleration near the limit position, thus fulfilling the requirements of this invention.

[0043] The swing structure of this invention eliminates the need for high-performance motors within the rotating mechanism 30. Furthermore, based on the lever principle, since the rotating mechanism 30 drives the third connecting structure 24, which is eccentrically arranged, the motor in this invention does not require a large torque, unlike traditional swing mechanisms where the motor outputs power directly to the pivot point 11. Additionally, this invention eliminates the need for complex motor control, such as controlling the motor's speed and direction of rotation at every moment. A conventional stepper motor can be used within the sophisticated mechanical structure, significantly reducing the production cost of the protein blot instrument. Preferably, the swing structure of this invention features multi-level speed adjustment to suit various working environments or meet different work requirements.

[0044] The above description is merely a specific embodiment of the invention, but the scope of protection of the invention is not limited thereto. Any changes or substitutions conceived without inventive effort should be included within the scope of protection of the invention. Therefore, the scope of protection of the invention should be determined by the scope defined in the claims.

Claims

1. A swing mechanism within a protein blot instrument, characterized in that, It includes a membrane support device, a linkage mechanism, and a rotating mechanism. The rotating mechanism is a driving component within the swing mechanism, used to provide kinetic energy to the swing mechanism. The rotating mechanism is connected to the linkage mechanism, and the rotating mechanism can drive the linkage mechanism to move. The linkage mechanism is connected to the membrane support device, and the rotating mechanism can drive the membrane support device to move through the linkage mechanism, realizing the repeated swinging of the membrane support device. The membrane support device has a pivot point, and the membrane support device can rotate around the pivot point; The pivot point is located on the vertical plane of the membrane support device; The position where the linkage mechanism is connected to the membrane support device is different from the position of the rotation fulcrum; The pivot point is located on the fixed plate, and the membrane support device is rotatably connected to the fixed plate through the pivot point; The fixing plate is provided with a fixing seat, the fixing seat is provided with two supporting ears, each of the two supporting ears is provided with a through hole for installing a pin, the pin is also fitted with a support plate, the support plate is also provided with a through hole, the support plate is connected to the membrane bearing device, and the pin is located at the rotation fulcrum of the membrane bearing device and the fixing seat. A sliding component is provided between the pin and the supporting ear and / or between the pin and the supporting plate; The linkage mechanism includes a first connecting structure, a second connecting structure, and a third connecting structure; wherein the first connecting structure is rotatably connected to the second connecting structure, the second connecting structure is rotatably connected to the third connecting structure, the first connecting structure is connected to the rotating mechanism, and the third connecting structure is connected to the membrane support device. The third connecting structure is located on the vertical plane of the membrane support device along its long side.

2. The swing mechanism in a protein blotting apparatus according to claim 1, characterized in that, The sliding component is a rolling bearing or a sliding bearing made of engineering plastics.

3. The swing mechanism within a protein blotting apparatus according to claim 1, characterized in that, The first connecting structure is in the shape of a disk.

4. The swing mechanism in a protein blotting apparatus according to claim 1, characterized in that, When the membrane support is in a horizontal position, the rotating mechanism is located on the lower side of the third connecting structure.

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