Sample analysis device and control method

Abstract

The embodiment of the present application provides a sample analysis device and a data processing method, wherein the data processing method is applied to the sample analysis device, the sample analysis device comprises: a reaction component having at least one placement site, the placement site is used for placing a reaction cup and incubating a reaction liquid in the reaction cup; and a light measurement component is used for performing light measurement on the incubated reaction liquid to obtain detection data, wherein the detection data comprises reaction data of a sample and a reference signal used for assisting in screening the reaction data; the method extracts corresponding to-be-analyzed reaction data from the reaction data according to the reference signal; and detects whether the to-be-analyzed reaction data has abnormal data; when the to-be-analyzed reaction data has abnormal data, the abnormal data is corrected to obtain valid to-be-analyzed reaction data, so that relatively accurate to-be-analyzed reaction data in a reaction process of a reactant can be obtained, and the accuracy of a data analysis result is ensured.

Description

Technical Field

[0001] This application relates to the field of medical devices, and more particularly to a sample analysis device and a data processing method. Background Technology

[0002] A sample analysis device is used to measure and analyze samples. Typically, sample analysis devices include cell analyzers, biochemical analyzers, and immunoassay analyzers. The basic principle of a sample analysis device is to periodically measure the reaction data of the reactants during the reaction process to obtain a reaction curve. Based on information such as the amplitude and rate of change of the curve, the corresponding measurement results are obtained.

[0003] For example, a biochemical analyzer obtains an absorbance response curve by periodically measuring the absorbance of reactants during the reaction process. Based on the amplitude and rate of change of the absorbance response curve, the corresponding absorbance measurement results can be obtained. Each data point on this absorbance response curve represents the absorbance obtained by photoelectric acquisition of the reactants within the reaction vessel as it rotates past a photometer.

[0004] Therefore, the accuracy of the measurement results from the sample analysis device depends on the precision of the reaction data of the reactant reaction process obtained.

[0005] Therefore, how to obtain relatively accurate reaction data during the reaction process of reactants in order to ensure the accuracy of data analysis results is a popular research topic in the field. Summary of the Invention

[0006] The main objective of this application is to provide a sample analysis device and a data processing method, which aims to obtain relatively accurate reaction data to be analyzed during the reaction process of reactants.

[0007] In a first aspect, embodiments of this application provide a sample analysis apparatus, comprising:

[0008] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0009] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0010] A memory for storing the aforementioned detection data and computer program; and

[0011] A processor, configured to invoke a computer program in the memory to execute:

[0012] Extract the corresponding reaction data to be analyzed from the reaction data based on the reference signal;

[0013] Detect whether there is any abnormal data in the reaction data to be analyzed;

[0014] When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed.

[0015] Secondly, embodiments of this application provide a sample analysis apparatus, comprising:

[0016] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0017] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0018] A memory for storing the aforementioned detection data and computer program; and

[0019] A processor, configured to invoke a computer program in the memory to execute:

[0020] Acquire the calibration parameters between the reaction data and the reference signal;

[0021] The correspondence between the reaction data and the reference signal is adjusted according to the calibration parameters.

[0022] Thirdly, embodiments of this application also provide a data processing method applied to a sample analysis device, the sample analysis device comprising:

[0023] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0024] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0025] The method includes:

[0026] Extract the corresponding reaction data to be analyzed from the reaction data based on the reference signal;

[0027] Detect whether there is any abnormal data in the reaction data to be analyzed;

[0028] When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed.

[0029] Fourthly, this application also provides a data processing method applied to a sample analysis device, the sample analysis device comprising:

[0030] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0031] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0032] The method includes: acquiring calibration parameters between the reaction data and the reference signal; and adjusting the correspondence between the reaction data and the reference signal according to the calibration parameters.

[0033] This application provides a sample analysis device and a data processing method. The data processing method is applied to the sample analysis device, which includes: a reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup; and a photometric component for performing photometric measurements on the incubated reaction liquid to obtain detection data. The detection data includes sample reaction data and a reference signal for assisting in screening the reaction data. The method extracts corresponding reaction data to be analyzed from the reaction data based on the reference signal and detects whether there is abnormal data in the reaction data to be analyzed. When abnormal data is found, the abnormal data is corrected to obtain valid reaction data to be analyzed, thereby obtaining relatively accurate reaction data to be analyzed during the reaction process and ensuring the accuracy of the data analysis results. Attached Figure Description

[0034] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 This is a schematic structural block diagram of a sample analysis device provided in an embodiment of this application;

[0036] Figure 2 This is a schematic diagram of the structure of the reaction component and the photometric component of the sample analysis device provided in the embodiments of this application.

[0037] Figure 3 This is a schematic diagram showing the correspondence between the reaction data curve and the reference signal line provided in the embodiments of this application;

[0038] Figure 4 This is a flowchart illustrating the steps of a data processing method provided in an embodiment of this application;

[0039] Figure 5 This is a schematic flowchart of the data screening step for the reaction data to be analyzed in a data processing method provided in an embodiment of this application;

[0040] Figure 6 This is a graph showing the relationship between sampling time and sampling electrical signal generated from reaction data of a single reaction vessel for one cycle, as provided in an embodiment of this application.

[0041] Figure 7A This is a flowchart illustrating the steps of an anomaly data analysis method provided in the embodiments of this application.

[0042] Figure 7B This is a graph showing the relationship between the sampling time and the sampling electrical signal generated from the reaction data of three adjacent cycles of a single reaction vessel provided in this application embodiment;

[0043] Figure 7C This is a flowchart illustrating another step in the abnormal data analysis method provided in the embodiments of this application;

[0044] Figure 7D This is a flowchart illustrating the steps of another abnormal data analysis method provided in the embodiments of this application;

[0045] Figure 8 This is a schematic diagram of abnormal data analysis and processing in the data processing method provided in the embodiments of this application;

[0046] Figure 9 This is a flowchart of the abnormal data processing steps in the data processing method provided in the embodiments of this application;

[0047] Figure 10 This is a flowchart of the steps of a data processing method provided in another embodiment of this application;

[0048] Figure 11 This is a flowchart of the steps for obtaining calibration parameters in a data processing method provided in another embodiment of this application;

[0049] Figure 12 This is a schematic diagram of obtaining calibration parameters based on reaction data and reference signals in a data processing method provided in another embodiment of this application. Detailed Implementation

[0050] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0051] In the description of this application, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0052] It should be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the scope of the application. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0053] This application provides a sample analysis device, a data processing method, and a storage medium. The data processing method can be applied to the sample analysis device, which includes a reaction component with at least one placement position for placing a reaction vessel and incubating the reaction liquid within it; and a photometric component for performing photometric measurements on the incubated reaction liquid to obtain detection data. The detection data includes sample reaction data and a reference signal used to assist in screening the reaction data. In one embodiment, the method extracts corresponding reaction data to be analyzed from the reaction data based on the reference signal; detects whether abnormal data exists in the reaction data to be analyzed; and corrects the abnormal data to obtain valid reaction data to be analyzed. By correcting the abnormal data, relatively accurate reaction data to be analyzed during the reaction process can be obtained, ensuring the accuracy of the data analysis results. In another embodiment, the method obtains calibration parameters between the reaction data and the reference signal; and adjusts the correspondence between the reaction data and the reference signal based on the calibration parameters. The correspondence between the reaction data and the reference signal is calibrated by calibration parameters, so that the corresponding reaction data to be analyzed can be screened out with the aid of the calibrated reference signal. In this way, the accuracy of the data analysis results of the sample analysis device can be effectively improved by using the screened reaction data to be analyzed.

[0054] The following detailed description of some embodiments of this application is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0055] Please see Figure 1 , Figure 1 This is a schematic block diagram of a sample analysis device provided in an embodiment of this application. The sample analysis device is used to execute the data processing method provided in this application.

[0056] like Figure 1 As shown, the sample analyzer 100 includes a reaction component 10, an optical measurement component 20, a memory 30, and a processor 40. The reaction component 10 has at least one placement position for placing a reaction cup M and incubating the reaction liquid within the reaction cup M. For example, the reaction component 10 can be a reaction disk, which has a disk-shaped structure and one or more placement positions for placing the reaction cup M. The reaction disk can rotate and drive the reaction cup M in its placement position to rotate, thereby managing the reaction cup M and the reaction liquid within the reaction cup M within the reaction disk.

[0057] The photometric component 20 is used to perform photometric measurements on the incubated reaction solution to obtain sample detection data. This detection data includes the sample's reaction data and a reference signal used to assist in screening the reaction data. For example, the photometric component 20 detects the luminescence intensity of the reaction solution to obtain the corresponding reaction data and the reference signal used to assist in screening the reaction data. After obtaining the corresponding analyte data from the reaction data based on the reference signal, the concentration of the analyte component in the sample is calculated based on the analyte data using a calibration curve.

[0058] Please see Figure 2 In some embodiments, the photometric component 20 includes a light source 201, a photoelectric switch 202, a photodetector 203, and a code disk assembly 204. The light source 201 emits a light signal towards the reaction vessel M; the photodetector 203 acquires corresponding reaction data based on the light signal passing through the reaction vessel M; and the code disk assembly 204 triggers the photoelectric switch 202 to output a corresponding reference signal for assisting in the screening of reaction data.

[0059] Specifically, the reaction component 10 is connected to a driving device, which includes, but is not limited to, a motor. Driven by the driving device, the reaction component 10 can rotate around point O. The reaction component 10 has multiple placement positions, each corresponding to a reaction cup M. A light source 201 and a photodetector 203 are respectively positioned on opposite sides of the reaction cup M, such that the light path formed between the light source 201 and the photodetector 203 passes through the reaction cup M. When the reaction component 10 rotates, the light signal emitted by the light source 201 passes through the corresponding reaction cup M sequentially. The photodetector 203 collects the light signal passing through the reaction cup M to obtain the corresponding reaction data, and processes and analyzes the reaction data to obtain the corresponding detection results. This reaction data includes, but is not limited to, the absorbance measurement value of the reaction liquid in the reaction cup M.

[0060] To reduce the error in the obtained detection results, it is necessary to filter out the corresponding reaction data to be analyzed from the reaction data. In this embodiment, the data filtering is achieved by using a reference signal corresponding to the reaction data.

[0061] Specifically, the code disk assembly 204 is circumferentially and evenly spaced with multiple code teeth. Driven by the driving device, it rotates synchronously with the reaction component 10. When the reaction component 10 rotates, the code teeth of the code disk assembly 204 periodically block the light path of the photoelectric switch 202, thereby periodically triggering the photoelectric switch 202. That is, the light path of the photoelectric switch 202 is sequentially cut off and connected by the code disk assembly 204, thus periodically outputting a corresponding electrical signal. Based on this electrical signal, a corresponding reference signal can be obtained. The edge 2041 of each code tooth is the light path of the photoelectric switch 202, which is sequentially blocked by the code disk assembly. The critical position for cutting off and connecting component 204 is such that when the code disk assembly 204 rotates to the edge 2041 of each code tooth, the photoelectric switch 202 is triggered. At this time, the photoelectric switch 202 outputs a jump signal corresponding to the edge 2041 of each code tooth of the code disk assembly 204. Between two adjacent code teeth of the code disk assembly 204, the photoelectric switch 202 is not triggered, and a stable signal is output. The processor 40 or other processing module records the correspondence between the reference signal and the reaction data, and the corresponding reaction data to be analyzed can be screened from the reaction data according to the reference signal.

[0062] like Figure 3As shown, curve A represents the relationship between the light signal emitted by light source 201 and the reaction cup M, and the electrical signal generated by photodetector 203 after periodically collecting the light signal passing through reaction cup M. In this figure, the horizontal axis of curve A represents the sampling time T, and the vertical axis represents the electrical signal value collected at the corresponding sampling time point. This electrical signal can be either a voltage signal or a current signal. Square wave line B represents the reference signal output after each code tooth of code disk assembly 204 periodically triggers photoelectric switch 202. Point B1 is the transition signal corresponding to when the edge of the code tooth triggers photoelectric switch 202. In this figure, the horizontal axis of square wave line B represents the trigger time T, and the vertical axis represents the reference signal generated based on the electrical signal output by photoelectric switch at the corresponding trigger time point.

[0063] By adjusting the sample analysis device 100, the timing of the transition signal of the reference signal of the photoelectric switch 202 is made to correspond to the timing of the light path formed between the light source 201 and the photodetector 203 passing through the preset position of the reaction cup M. Therefore, the reaction data generated when the light path between the light source 201 and the photodetector 203 passes through the preset position of the reaction cup M is the reaction data to be analyzed. This preset position can be set as needed, for example, it can be a position within a preset range around the centerline L of the reaction cup M.

[0064] Alternatively, by debugging the sample analysis device 100, the correspondence between the time point when the reference signal of the photoelectric switch 202 is generated and the time point when the light path formed between the light source 201 and the photodetector 203 passes through the preset position of the reaction cup M is established, and this correspondence is stored. When the processor 40 calls this correspondence, it can filter out the corresponding reaction data to be analyzed from the reaction data according to the reference signal. The reaction data to be analyzed is the reaction data generated when the light path formed between the light source 201 and the photodetector 203 passes through the preset position of the reaction cup M.

[0065] In summary, the processor 40 can locate the corresponding reaction data to be analyzed from the reaction data based on the jump signal in the reference signal. This ensures that when multiple data acquisitions are performed for each reaction vessel M, the reaction data occurring at the same preset position in the circumferential direction of the reaction vessel M can be extracted from each acquired reaction data as the reaction data to be analyzed. This can effectively reduce the errors caused by the inhomogeneity of the reaction vessel M and / or the reactants.

[0066] like Figure 3 As shown, region C, corresponding to the two dashed lines on curve A, is the region of electrical signals corresponding to the reaction data to be analyzed that is to be screened and obtained.

[0067] In some embodiments, the photodetector 203 includes a photoelectric sensor, a conditioning circuit connected to the photoelectric sensor, and an AD converter connected to the conditioning circuit. The photoelectric sensor is used to collect the light signal passing through the reaction cup M and convert the light signal into a corresponding electrical signal, which can be a current signal or a voltage signal, without limitation herein.

[0068] The conditioning circuit is connected to the photoelectric sensor and is used to preprocess the electrical signal output by the photoelectric sensor. The preprocessing includes, but is not limited to, electrical signal conversion, electrical signal amplification, and electrical signal filtering, so as to output a conditioned electrical signal. The AD converter is connected to the conditioning circuit and is used to convert the conditioned electrical signal output by the conditioning circuit into a digital domain to obtain the corresponding response data.

[0069] In this embodiment, the bandwidth of the conditioning circuit is designed to be on the order of kilohertz (kHz) to ensure that the signal settling time does not become a system bottleneck. The acquisition rate of the AD converter is several times higher than the bandwidth of the conditioning circuit, ensuring that both signals and noise within the Nyquist frequency range are completely acquired and entered into the digital domain.

[0070] The processor 40 can be a Central Processing Unit (CPU), but it can also be other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor.

[0071] Memory 30 can be volatile memory, such as random access memory (RAM); or non-volatile memory, such as read-only memory (ROM), flash memory, hard disk drive (HDD), or solid-state drive (SSD); or a combination of the above types of memory. Memory 30 is used to store computer programs and can provide instructions and data to processor 40.

[0072] In one embodiment, the processor 40 is configured to execute a computer program stored in the memory 30, and when executing the computer program, it can perform the following method steps:

[0073] Extract the corresponding reaction data to be analyzed from the reaction data based on the reference signal;

[0074] Detect whether there is any abnormal data in the reaction data to be analyzed;

[0075] When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed.

[0076] In some embodiments, when the processor 40 extracts the corresponding reaction data to be analyzed from the reaction data according to the reference signal, it includes:

[0077] The screening position of the reaction data is determined based on the reference signal;

[0078] The reaction data to be analyzed is extracted from the reaction data based on the selected location.

[0079] In some embodiments, when the processor 40 extracts reaction data to be analyzed from the reaction data according to the screening position, it includes:

[0080] Based on the selected location, extract reaction data within a preset range, and perform noise reduction processing on the extracted reaction data;

[0081] The noise-reduced reaction data is used as the corresponding reaction data to be analyzed.

[0082] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0083] Acquire reference reaction data from at least two adjacent periods, wherein the reference reaction data are reaction data corresponding to the reaction data to be analyzed acquired in different data acquisition periods;

[0084] Based on the reference reaction data, determine whether there is any abnormal data in the reaction data to be analyzed.

[0085] In some embodiments, when the processor 40 determines whether there is abnormal data in the reaction data to be analyzed based on the reference reaction data, it includes:

[0086] At least two first fitted lines are generated based on the reference reaction data from at least two adjacent periods;

[0087] A second fitting line generated based on the reaction data to be analyzed;

[0088] When the similarity between two first fitted lines exceeds a first threshold, and the similarity between the second fitted line and any of the first fitted lines is less than a second threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0089] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0090] The corresponding reaction fitting line is generated by fitting the reaction data to be analyzed.

[0091] Determine whether the similarity between the reaction fitting line and a preset reaction fitting line exceeds a preset first threshold.

[0092] When the similarity between the reaction fitting line and the preset reaction fitting line does not exceed a preset threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0093] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0094] Multiple sets of sub-data are obtained from the reaction data to be analyzed;

[0095] Generate a corresponding sub-fit trend line based on each set of sub-data;

[0096] Determine whether the similarity between any two of the subfitted trend lines is less than a third threshold;

[0097] When the similarity between any two of the subfit trend lines is less than the third threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0098] In some embodiments, when the processor 40 corrects the anomalous data to obtain valid reaction data to be analyzed, it includes:

[0099] A trend line is fitted based on the reaction data to be analyzed adjacent to the abnormal data;

[0100] Obtain corrected data corresponding to the abnormal data based on the trend line;

[0101] The corrected data is used to replace the anomalous data to obtain valid reaction data for analysis.

[0102] In some embodiments, the processor 40 is also configured to perform:

[0103] Acquire the calibration parameters between the reaction data and the reference signal;

[0104] The correspondence between the reaction data and the reference signal is adjusted according to the calibration parameters.

[0105] In some embodiments, the reference signal includes a transition signal, and the processor 40, when acquiring calibration parameters between the reaction data and the reference signal, includes:

[0106] Generate a corresponding reaction curve based on the reaction data, and determine the calibration position of the reaction curve;

[0107] Determine the transition position corresponding to the transition signal in the reference signal;

[0108] The calibration parameters are determined based on the calibration position and the jump position.

[0109] In some embodiments, the response curve includes a peak segment and a first trough segment and a second trough segment located on opposite sides of the peak segment. When determining the calibration position of the response curve, the processor includes:

[0110] Obtain a first reference point at a preset distance from the first wave crest segment in the first wave trough segment;

[0111] In the second trough segment, a second reference point is obtained at a preset distance from the crest segment;

[0112] Obtain the midpoint of the line connecting the first reference point and the second reference point;

[0113] A calibration point corresponding to the midpoint is determined in the wave crest segment, and the location of the calibration point is used as the reference position.

[0114] In another embodiment, the processor 40 is configured to execute a computer program stored in the memory 30, and when executing the computer program, it can perform the following method steps:

[0115] Acquire the calibration parameters between the reaction data and the reference signal;

[0116] The correspondence between the reaction data and the reference signal is adjusted according to the calibration parameters.

[0117] In some embodiments, the reference signal includes a transition signal, and the processor 40, when acquiring calibration parameters between the reaction data and the reference signal, includes:

[0118] Generate a corresponding reaction curve based on the reaction data, and determine the calibration position of the reaction curve;

[0119] Determine the transition position corresponding to the transition signal in the reference signal;

[0120] The calibration parameters are determined based on the calibration position and the jump position.

[0121] In some embodiments, the processor 40 is also configured to perform:

[0122] Extract the corresponding reaction data to be analyzed from the reaction data based on the reference signal;

[0123] Detect whether there is any abnormal data in the reaction data to be analyzed;

[0124] When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed.

[0125] In some embodiments, when the processor 40 extracts the corresponding reaction data to be analyzed from the reaction data according to the reference signal, it includes:

[0126] The screening position of the reaction data is determined based on the reference signal;

[0127] The reaction data to be analyzed is extracted from the reaction data based on the selected location.

[0128] In some embodiments, when the processor 40 extracts reaction data to be analyzed from the reaction data according to the screening position, it includes:

[0129] Based on the selected location, extract reaction data within a preset range, and perform noise reduction processing on the extracted reaction data;

[0130] The noise-reduced reaction data is used as the corresponding reaction data to be analyzed.

[0131] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0132] Acquire reference reaction data from at least two adjacent periods, wherein the reference reaction data are reaction data corresponding to the reaction data to be analyzed acquired in different data acquisition periods;

[0133] Based on the reference reaction data, determine whether there is any abnormal data in the reaction data to be analyzed.

[0134] In some embodiments, when the processor 40 determines whether there is abnormal data in the reaction data to be analyzed based on the reference reaction data, it includes:

[0135] At least two first fitted lines are generated based on the reference reaction data from at least two adjacent periods;

[0136] A second fitting line generated based on the reaction data to be analyzed;

[0137] When the similarity between two first fitted lines exceeds a first threshold, and the similarity between the second fitted line and any of the first fitted lines is less than a second threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0138] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0139] The corresponding reaction fitting line is generated by fitting the reaction data to be analyzed.

[0140] Determine whether the similarity between the reaction fitting line and a preset reaction fitting line exceeds a preset first threshold.

[0141] When the similarity between the reaction fitting line and the preset reaction fitting line does not exceed a preset threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0142] In some embodiments, when the processor 40 detects whether there is abnormal data in the reaction data to be analyzed, it includes:

[0143] Multiple sets of sub-data are obtained from the reaction data to be analyzed;

[0144] Generate a corresponding sub-fit trend line based on each set of sub-data;

[0145] Determine whether the similarity between any two of the subfitted trend lines is less than a third threshold;

[0146] When the similarity between any two of the subfit trend lines is less than the third threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0147] In some embodiments, when the processor 40 corrects the anomalous data to obtain valid reaction data to be analyzed, it includes:

[0148] A trend line is fitted based on the reaction data to be analyzed adjacent to the abnormal data;

[0149] Obtain corrected data corresponding to the abnormal data based on the trend line;

[0150] The corrected data is used to replace the anomalous data to obtain valid reaction data for analysis.

[0151] The following embodiments, in conjunction with the working principle of the sample analysis device, provide a detailed description of a data processing method provided in this application.

[0152] Please see Figure 4 , Figure 4 This is a schematic flowchart of a data processing method provided in an embodiment of this application.

[0153] This data processing method can be applied to a sample analysis device, which includes:

[0154] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0155] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0156] The method includes steps S101 to S103, as follows:

[0157] Step S101: Extract the corresponding reaction data to be analyzed from the reaction data according to the reference signal.

[0158] There is a corresponding correlation between the reference signal and the reflected data. Based on the reference signal, the corresponding data can be filtered out from the reflected data.

[0159] like Figure 5 As shown, in some embodiments, step S101 includes steps S1011 to S1012.

[0160] Wherein, step S1011: determine the screening position of the reaction data according to the reference signal;

[0161] Step S1012: Extract the reaction data to be analyzed from the reaction data according to the screening position.

[0162] For example, the reaction data collected by the photometric component includes some impurity data, which needs to be filtered. The reference signal is a positioning signal used to filter the reaction data. Based on the reference signal, the filtering position of the reaction data that needs to be filtered can be determined. The reaction data corresponding to the filtering position is extracted from the filtering position, thereby extracting higher quality reaction data to be analyzed.

[0163] like Figure 3 As shown, A is the electrical signal curve corresponding to the reaction data, and B is the square wave line generated based on the reference signal. The reaction data position A1 corresponding to the jump signal B1 of the reference signal B is determined. Thus, the corresponding reaction data can be extracted from the curve A based on the selection position A1 as the reaction data to be analyzed, such as the reaction data corresponding to region C in the curve A.

[0164] In some embodiments, extracting reaction data to be analyzed from the reaction data according to the screening position includes:

[0165] Based on the selected location, extract reaction data within a preset range, and perform noise reduction processing on the extracted reaction data;

[0166] The noise-reduced reaction data is used as the corresponding reaction data to be analyzed.

[0167] For example, after determining the screening position, starting from the screening position, the reaction data within a preset range corresponding to the preset distance from the screening position is obtained, and the reaction data is denoised, and the denoised reaction data is used as the corresponding reaction data to be analyzed.

[0168] like Figure 6 As shown, the extraction of reaction data to be analyzed is illustrated using the reaction data of one cycle from a single reaction vessel as an example. Figure 6 The curve in the middle is a graph showing the relationship between the sampling time and the sampled electrical signal generated from the reaction data of a single reaction vessel for one cycle. The horizontal axis of the curve is the sampling time T, and the vertical axis is the electrical signal value corresponding to the reaction data collected at the corresponding sampling time point.

[0169] To obtain more comprehensive data, the photodetector uses a high acquisition rate when collecting reaction data, ensuring that both effective and noise signals are acquired. In the a1 and a3 regions between adjacent reaction cups M, the sample analysis device needs to establish the signal more quickly, and noise levels are not a concern at this stage; therefore, high-bandwidth digital filtering is used in a1 and a3 regions. However, in the photometric flat region a2, the effective signal bandwidth is extremely low, and the sample analysis device requires extremely low noise levels. Therefore, low-bandwidth digital filtering is used in this region for further noise reduction. This achieves an ideal balance between noise and timing. High-bandwidth digital filtering refers to a filter bandwidth higher than a preset bandwidth, while low-bandwidth digital filtering refers to a filter bandwidth less than or equal to a preset bandwidth. The preset bandwidth can be set as needed.

[0170] Step S102: Detect whether there is any abnormal data in the reaction data to be analyzed.

[0171] After obtaining the reaction data to be analyzed, in order to ensure the accuracy of the data analysis results based on the reaction data, it is necessary to check whether there is any abnormal data in the reaction data to be analyzed.

[0172] Please see Figure 7A In some embodiments, step S102 includes steps S1021a to S1022a.

[0173] Step S1021a: Obtain reference reaction data for at least two adjacent periods, wherein the reference reaction data are reaction data corresponding to the reaction data to be analyzed, obtained in different data acquisition periods.

[0174] Step S1022a: Determine whether there is any abnormal data in the reaction data to be analyzed based on the reference reaction data.

[0175] In some embodiments, determining whether there is abnormal data in the reaction data to be analyzed based on the reference reaction data includes:

[0176] At least two first fitted lines are generated based on the reference reaction data from at least two adjacent periods;

[0177] A second fitting line generated based on the reaction data to be analyzed;

[0178] When the similarity between two first fitted lines exceeds a first threshold, and the similarity between the second fitted line and any of the first fitted lines is less than a second threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0179] like Figure 7B As shown, for example, the reaction data X to be analyzed is the data obtained in the Tth cycle of the reaction vessel. When detecting whether there is a problem with the reaction data X to be analyzed, the reaction data X-1 corresponding to the reaction data X to be analyzed in the T-1th cycle and the reaction data X+1 corresponding to the reaction data X to be analyzed in the T+1th cycle of the same reaction vessel are obtained. Using the reaction data X-1 and the reaction data X+1 as reference reaction data, a corresponding first fitting curve is generated. Based on the second fitting line generated from the reaction data X to be analyzed, when the similarity between the two first fitting lines exceeds a first threshold, and the similarity between the second fitting line and any of the first fitting lines is less than a second threshold, it is determined that there is abnormal data in the reaction data X to be analyzed. Figure 7B As shown, the middle part of the second fitted curve X generated from the reaction data X to be analyzed is concave towards the B square wave line.

[0180] Please see Figure 7C In some embodiments, step S102 includes steps S1021b to S1023b.

[0181] Step S1021b: Fit the reaction data to be analyzed to generate a corresponding reaction fitting line;

[0182] Step S1022b: Determine whether the similarity between the reaction fitting line and the preset reaction fitting line exceeds a preset first threshold;

[0183] Step S1023b: When the similarity between the reaction fitting line and the preset reaction fitting line does not exceed the preset threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0184] For example, the sample analysis device stores a preset reaction fitting line generated based on the corresponding normal reaction data. After obtaining the reaction data to be analyzed, the device fits the reaction data to generate the corresponding reaction fitting line and determines whether the similarity between the reaction fitting curve and the preset reaction fitting line exceeds a preset threshold. If the similarity does not exceed the preset threshold, it is determined that the reaction data to be analyzed contains abnormal data; otherwise, if the similarity exceeds the preset threshold, it is determined that the reaction data to be analyzed does not contain abnormal data.

[0185] Please see Figure 7D In some embodiments, step S102 includes steps S1021c to S1024c.

[0186] Step S1021c: Obtain multiple sets of sub-data from the reaction data to be analyzed;

[0187] Step S1022c: Generate the corresponding sub-fit trend line based on each group of sub-data;

[0188] Step S1023c: Determine whether the similarity between any two of the subfit trend lines is less than the third threshold;

[0189] Step S1024c: When the similarity between any two of the subfit trend lines is less than the third threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0190] Please see Figure 8 For example, reaction data X to be analyzed is obtained from reaction data, and multiple sets of sub-data are extracted from reaction data X, namely D1, D2, D3...Dn, or the reaction data X to be analyzed is divided into multiple sets of sub-data with numbers of D1, D2, D3...Dn, and the data difference of each set of sub-data is less than the difference threshold.

[0191] In this embodiment, the example of obtaining three sets of sub-data, namely D1, D2, and D3, from the reaction data to be analyzed is used for illustration.

[0192] After obtaining three sets of sub-data D1, D2, and D3 from the reaction data X to be analyzed, corresponding sub-fitting trend lines are generated based on each set of sub-data D1, D2, and D3. It is determined whether the similarity between any two sub-fitting trend lines is less than the corresponding third threshold. If the similarity between any two sub-fitting trend lines is less than the third threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

[0193] Step S103: When there is abnormal data in the reaction data to be analyzed, correct the abnormal data to obtain valid reaction data to be analyzed.

[0194] If there are abnormal data in the reaction data to be analyzed, the abnormal data needs to be processed in order to obtain the corresponding valid reaction data to be analyzed.

[0195] Please see Figure 9 In some embodiments, correcting the anomalous data to obtain valid reaction data to be analyzed includes:

[0196] Step S1031: Fit a trend line based on the reaction data to be analyzed adjacent to the abnormal data;

[0197] Step S1032: Obtain the corrected data corresponding to the abnormal data based on the trend line;

[0198] Step S1033: Replace the abnormal data with the corrected data to obtain valid reaction data to be analyzed.

[0199] like Figure 8 As shown, for example, when an abnormal data w1 is detected in the reaction data X to be analyzed, a fitting trend line y is generated based on the reaction data w0 and w2 adjacent to the abnormal data w1. On the trend line y, the corrected data w corresponding to the abnormal data w1 is generated by interpolation. The abnormal data w1 is replaced by the corrected data w to obtain valid reaction data to be analyzed.

[0200] Alternatively, the outlier data w1 can be mapped onto the fitted trend line y to generate corresponding corrected data w, and the corrected data w can be used to replace the outlier data w1 to obtain valid response data to be analyzed.

[0201] By extracting the corresponding reaction data to be analyzed from the reaction data using a reference signal, the preliminary impurity removal process of the reaction data is achieved, ensuring the accuracy of the data analysis results. By correcting abnormal data in the reaction data to be analyzed, valid reaction data to be analyzed can be obtained. Thus, relatively accurate reaction data to be analyzed during the reaction process of the reactants can be obtained, ensuring the accuracy of the data analysis results.

[0202] Please see Figure 10 , Figure 10 A data processing method provided in another embodiment of this application is applied to a sample analysis device, the sample analysis device comprising:

[0203] A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup;

[0204] A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data;

[0205] The method includes steps S201 to S202, as follows:

[0206] Step S201: Obtain the calibration parameters between the reaction data and the reference signal.

[0207] The reference signal is used to assist in filtering the corresponding reaction data to be analyzed from the reaction data. Specifically, the corresponding data in the reaction data is located and extracted according to the reference signal to obtain the corresponding reaction data. Therefore, by obtaining the calibration parameters between the reaction data and the reference signal, the reaction data and the reference signal are calibrated using the calibration parameters to obtain an accurate correspondence between the reaction data and the reference signal.

[0208] Please see Figure 11 In some embodiments, the reference signal includes a transition signal, and step S201 includes steps S2011-2013.

[0209] Step S2011: Generate a corresponding reaction curve based on the reaction data, and determine the calibration position of the reaction curve.

[0210] The reaction data generated by the optical path formed between the light source and the photodetector passing through the preset position of the reaction cup is the reaction data to be analyzed. The preset position is the location within a preset range around the centerline L of the reaction cup; therefore, the required calibration position is the position on the reaction curve corresponding to the centerline L of the reaction cup.

[0211] In some embodiments, the response curve includes a peak segment and a first trough segment and a second trough segment located on opposite sides of the peak segment. Determining the calibration position of the response curve includes:

[0212] Obtain a first reference point at a preset distance from the first wave crest segment in the first wave trough segment;

[0213] In the second trough segment, a second reference point is obtained at a preset distance from the crest segment;

[0214] Obtain the midpoint of the line connecting the first reference point and the second reference point;

[0215] A calibration point corresponding to the midpoint is determined in the wave crest segment, and the location of the calibration point is used as the reference position.

[0216] like Figure 12As shown, the corresponding reaction curve A generated based on the reaction data includes a peak segment and a first trough segment and a second trough segment located on opposite sides of the peak segment. A first reference point x1 is obtained at a preset distance from the peak segment in the first trough segment, and a second reference point x2 is obtained at the same preset distance from the peak segment in the second trough segment. The midpoint O1 of the line connecting the first reference point x1 and the second reference point x2 is obtained. A calibration point O2 corresponding to the midpoint O1 is determined in the peak segment, and the position of the calibration point O2 is used as the reference position. The straight line containing O1 and O2 corresponds to the center line L of the reaction cup M.

[0217] Step S2012: Determine the transition position corresponding to the transition signal in the reference signal.

[0218] like Figure 12 As shown, a data filtering reference line is generated based on the reference signal B, and the transition position B1 corresponding to the transition signal in the data filtering reference line is determined.

[0219] Step S2013: Determine the calibration parameters based on the calibration position and the jump position.

[0220] During calibration, the jump position B1 and the calibration point O2 need to be aligned. Therefore, the corresponding calibration parameter α is obtained by calculating the offset between the jump position B1 and the calibration point O2.

[0221] Step S202: Adjust the correspondence between the reaction data and the reference signal according to the calibration parameters.

[0222] The reaction curve A or reference signal B formed by the reaction data is adjusted according to the jump position B1, calibration point O2 and calibration parameter α so that the jump position B1 and calibration point O2 correspond, so that the reaction parameters can be accurately selected directly according to the jump signal of reference signal B.

[0223] By acquiring calibration parameters between reaction data and reference signals, and using these parameters to calibrate the correspondence between them, the generation position of the transition signal in the reference signal is precisely aligned with the preset position of the reaction cup. This reduces the difficulty of equipment assembly and debugging. Furthermore, the calibrated reference signal allows for the accurate extraction of the corresponding reaction data to be analyzed from the reaction data, thereby effectively improving the accuracy of subsequent data processing.

[0224] like Figure 10 As shown, in some embodiments, after step S202, the data processing method further includes steps S203 to S205, as follows:

[0225] Step S203: Extract the corresponding reaction data to be analyzed from the reaction data according to the reference signal.

[0226] Step S203 and Figure 4 The steps in step S101 are the same and will not be repeated here.

[0227] Step S204: Detect whether there is any abnormal data in the reaction data to be analyzed.

[0228] Step S204 and Figure 4 The steps in step S102 are the same and will not be repeated here.

[0229] Step S205: When there is abnormal data in the reaction data to be analyzed, correct the abnormal data to obtain valid reaction data to be analyzed.

[0230] Step S205 and Figure 4 The steps in step S103 are the same and will not be repeated here.

[0231] This application also provides a storage medium for computer-readable storage, wherein the storage medium stores one or more programs that can be executed by one or more processors to implement the steps of any of the data processing methods provided in this application.

[0232] The storage medium can be an internal storage unit of the detection device described in the foregoing embodiments, such as the hard drive or memory of the detection device. Alternatively, the storage medium can be an external storage device of the detection device, such as a plug-in hard drive, Smart Media Card (SMC), Secure Digital (SD) card, or Flash Card equipped on the detection device.

[0233] It will be understood by those skilled in the art that all or some of the steps, systems, or apparatuses disclosed above, and their functional modules / units, can be implemented as software, firmware, hardware, or suitable combinations thereof. In hardware embodiments, the division between functional modules / units mentioned in the above description does not necessarily correspond to the division of physical components; for example, a physical component may have multiple functions, or a function or step may be performed collaboratively by several physical components. Some or all physical components may be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit. Such software may be distributed on a computer-readable medium, which may include computer storage media (or non-transitory media) and communication media (or transient media). As is known to those skilled in the art, the term computer storage media includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data). Computer storage media include, but are not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disc (DVD) or other optical disc storage, magnetic cartridges, magnetic tape, disk storage or other magnetic storage devices, or any other medium that can be used to store desired information and can be accessed by a computer. Furthermore, it is well known to those skilled in the art that communication media typically contain computer-readable instructions, data structures, program modules, or other data in modulated data signals such as carrier waves or other transmission mechanisms, and may include any information delivery medium.

[0234] It should be understood that the term "and / or" as used in this specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, herein, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or system that includes that element.

[0235] The sequence numbers of the embodiments in this application are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments. The above descriptions are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A sample analysis device, characterized in that, include: A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup; A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data; A memory for storing the detection data and the computer program; and A processor, configured to invoke a computer program in the memory to execute: The screening position of the reaction data is determined based on the reference signal; Based on the filtering position, the corresponding reaction data to be analyzed is extracted from the reaction data of the first data acquisition cycle; Detect whether there is any abnormal data in the reaction data to be analyzed; When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed. The detection of whether there is abnormal data in the reaction data to be analyzed includes: Acquire reference reaction data for at least two data acquisition cycles adjacent to the first data acquisition cycle for the same reaction vessel, wherein the reference reaction data is reaction data corresponding to the reaction data to be analyzed in the first data acquisition cycle, acquired from each of the at least two data acquisition cycles based on the reference signal; At least two first fitting lines are generated based on the reference reaction data from the at least two data acquisition cycles; a second fitting line is generated based on the reaction data to be analyzed from the first data acquisition cycle; when the similarity between the two first fitting lines exceeds a first threshold, and the similarity between the second fitting line and any one of the two first fitting lines is less than a second threshold, it is determined that there is abnormal data in the reaction data to be analyzed. and / or A corresponding reaction fitting line is generated based on the reaction data to be analyzed in the first data acquisition period; it is determined whether the similarity between the reaction fitting line and the preset reaction fitting line exceeds a preset first threshold; when the similarity between the reaction fitting line and the preset reaction fitting line does not exceed the preset first threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

2. The sample analysis device according to claim 1, characterized in that, When the processor extracts the reaction data to be analyzed from the reaction data according to the filtering position, it includes: Based on the selected location, extract reaction data within a preset range, and perform noise reduction processing on the extracted reaction data; The noise-reduced reaction data is used as the corresponding reaction data to be analyzed.

3. The sample analysis apparatus according to claim 1, characterized in that, When the processor detects whether there is abnormal data in the reaction data to be analyzed, it includes: Multiple sets of sub-data are obtained from the reaction data to be analyzed; Generate a corresponding sub-fit trend line based on each set of sub-data; Determine whether the similarity between any two of the subfitted trend lines is less than a third threshold; When the similarity between any two of the subfit trend lines is less than the third threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

4. The sample analysis apparatus according to claim 1, characterized in that, When the processor corrects the anomalous data to obtain valid reaction data to be analyzed, it includes: A trend line is fitted based on the reaction data to be analyzed adjacent to the abnormal data; Obtain corrected data corresponding to the abnormal data based on the trend line; The corrected data is used to replace the anomalous data to obtain valid reaction data for analysis.

5. The sample analysis apparatus according to claim 1, characterized in that, The processor is also used to perform: Acquire the calibration parameters between the reaction data and the reference signal; The correspondence between the reaction data and the reference signal is adjusted according to the calibration parameters.

6. The sample analysis apparatus according to claim 5, characterized in that, The reference signal includes a transition signal, and when the processor acquires the calibration parameters between the reaction data and the reference signal, it includes: Generate a corresponding reaction curve based on the reaction data, and determine the calibration position of the reaction curve; Determine the transition position corresponding to the transition signal in the reference signal; The calibration parameters are determined based on the calibration position and the jump position.

7. The sample analysis apparatus according to any one of claims 1-6, characterized in that, The optical measurement component includes: Photoelectric switch; A light source is used to emit light signals to the reaction vessel; A photodetector is used to acquire the reaction data based on the light signal passing through the reaction cup; A code disk assembly is used to trigger the photoelectric switch so that the photoelectric switch outputs the reference signal.

8. A data processing method, characterized in that, Applied to a sample analysis device, the sample analysis device comprising: A reaction component having at least one placement position for placing a reaction cup and incubating the reaction liquid in the reaction cup; A photometric component is used to perform photometric measurements on the incubated reaction solution to obtain detection data, wherein the detection data includes the reaction data of the sample and a reference signal used to assist in screening the reaction data; The method includes: The screening position of the reaction data is determined based on the reference signal; Based on the filtering position, the corresponding reaction data to be analyzed is extracted from the reaction data of the first data acquisition cycle; Detect whether there is any abnormal data in the reaction data to be analyzed; When abnormal data is found in the reaction data to be analyzed, the abnormal data is corrected to obtain valid reaction data to be analyzed. The detection of whether there is abnormal data in the reaction data to be analyzed includes: Acquire reference reaction data for at least two data acquisition cycles adjacent to the first data acquisition cycle for the same reaction vessel, wherein the reference reaction data is reaction data corresponding to the reaction data to be analyzed in the first data acquisition cycle, acquired from each of the at least two data acquisition cycles based on the reference signal; At least two first fitting lines are generated based on the reference reaction data from the at least two data acquisition cycles; a second fitting line is generated based on the reaction data to be analyzed from the first data acquisition cycle; when the similarity between the two first fitting lines exceeds a first threshold, and the similarity between the second fitting line and any one of the two first fitting lines is less than a second threshold, it is determined that there is abnormal data in the reaction data to be analyzed. and / or A corresponding reaction fitting line is generated based on the reaction data to be analyzed in the first data acquisition period; it is determined whether the similarity between the reaction fitting line and the preset reaction fitting line exceeds a preset first threshold; when the similarity between the reaction fitting line and the preset reaction fitting line does not exceed the preset first threshold, it is determined that there is abnormal data in the reaction data to be analyzed.

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