A device for positioning and quantifying injury of the tibialis anterior muscle of a rodent

Abstract

The application discloses a device for positioning and quantifying a tibialis anterior muscle injury model of rodents, and belongs to the technical field of medical equipment, which comprises a shearing assembly and a positioning and measuring device, the positioning and measuring device is arranged on the shearing assembly, the positioning and measuring device comprises a measuring assembly for defining the size of a skeletal muscle injury and a positioning assembly for positioning a tibialis anterior muscle part, the measuring assembly is arranged on the positioning assembly, the measuring direction of the measuring assembly is perpendicular to the positioning direction of the positioning assembly, the positioning direction of the positioning assembly is perpendicular to the shearing direction of the shearing assembly, and the lengths of the two ends of the positioning assembly are equal to the length of the output end of the shearing assembly; the device can help researchers to position and quantify the establishment of a tibialis anterior muscle injury model of rodents.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical equipment, in particular to a tibialis anterior muscle positioning and quantitative injury model device for rodents. BACKGROUND

[0002] The muscle injury model of rodents (rats and mice) is the most widely used animal model for studying skeletal muscle repair and regeneration. It mainly causes a muscle injury groove in the tibialis anterior muscle belly of mice or rats. The degree of muscle injury directly affects the potential of muscle regeneration. If the muscle injury is slight, the regeneration is relatively rapid and the tissue after regeneration is complete. If the muscle injury is severe and even exceeds the self-regeneration level, the muscle regeneration is incomplete, resulting in a large amount of scar tissue generation. Therefore, it is necessary to standardize and limit the size of muscle injury.

[0003] In the prior art, the size of muscle injury of such animal models is reported differently in various literatures, and researchers use different methods in the modeling process, which seriously leads to the difficulty in standardizing the muscle injury model, the great difference in the results of injury repair, and the difficulty in data statistics. Therefore, a device for positioning and quantitatively establishing a tibialis anterior muscle injury model for rodents is needed. When the device is used, the small animal is placed in a supine position, the limbs are fixed, the tibialis anterior muscle belly on one side of the lower limb of the small animal is exposed by a shearing assembly, then the shearing assembly is positioned and fixed at the tibialis anterior muscle site by a positioning assembly, the size of the skeletal muscle injury range is limited by an adjusting and measuring assembly, thereby determining the range to be sheared by the shearing assembly, and finally the muscle is sheared by the shearing assembly, thereby facilitating the establishment of a muscle injury model, and achieving the effect of positioning and quantitatively establishing a tibialis anterior muscle injury model for rodents.

[0004] Application Content

[0005] The embodiment of the present application provides a tibialis anterior muscle positioning and quantitative injury model device for rodents to solve the above-mentioned technical problems.

[0006] The embodiment of the present application adopts the following technical scheme: a shearing assembly and a positioning and measuring device are included, the positioning and measuring device is arranged on the shearing assembly, the positioning and measuring device includes a measuring assembly for limiting the size of skeletal muscle injury and a positioning assembly for positioning the tibialis anterior muscle site, the measuring assembly is arranged on the positioning assembly, the measuring direction of the measuring assembly is perpendicular to the positioning direction of the positioning assembly, the positioning direction of the positioning assembly is perpendicular to the shearing direction of the shearing assembly, and the lengths of the two ends of the positioning assembly are equal to the length of the output end of the shearing assembly.

[0007] Further, the shearing assembly comprises two clamping bodies, a rotating shaft, an annular handle and an arc-shaped blade, the two clamping bodies are arranged in cross and connected by the rotating shaft at the contact end, the clamping body comprises a stop portion and a holding portion, the stop portion of the two clamping bodies is provided with the arc-shaped blade and arranged symmetrically, and the holding portion of the two clamping bodies is provided with the annular handle at the end for fingers to pass through.

[0008] Further, the measuring assembly comprises a sleeve, a scale and an adjusting screw, the sleeve penetrates the contact end of the two clamping bodies arranged in cross and is sleeved on the rotating shaft, the scale is in a cylindrical shape, the scale is provided with two scales symmetrically arranged inside the two ends of the sleeve and slidably matched with the sleeve, and the adjusting screw is provided with two adjusting screws symmetrically arranged and detachably sleeved on one end of each scale away from the sleeve.

[0009] Further, the positioning assembly comprises a positioning pile and a pile needle, the positioning pile is provided with two positioning piles vertically sleeved on the contact end of the two scales and the adjusting screw and slidably matched with the two scales and the adjusting screw, the two adjusting screws are located on the side of the two positioning piles away from the sleeve, the two positioning piles are provided with a sliding groove for the scale to slide up and down, the sliding groove is internally provided with a thread matched with the thread end of the adjusting screw, and the pile needle is provided with two pile needles arranged at the bottom of the positioning pile and arranged with the needle tip downward, and the needle tips of the two pile needles are located on the same horizontal line.

[0010] Further, one end of the holding portion of one of the clamping bodies close to the annular handle is provided with an angle controller, one side of the angle controller close to the annular handle is provided with an angle scale tooth and fixedly connected with the angle scale tooth, the holding portion of the other clamping body is provided with a slot hole through which the angle controller and the angle scale tooth pass, the slot hole is internally provided with an opening complementary to the shape of the angle control tooth, and the angle controller and the angle scale tooth are slidably matched in the slot hole.

[0011] Further, the angle controller and the angle scale tooth are sleeved with a blocking block, the blocking block is provided with two blocking blocks arranged on the two sides of one of the clamping bodies.

[0012] Further, the positioning pile is provided with a water drop calibrator, the water drop calibrator is in a spherical shape, filled with water, provided with a bubble in the water, the bubble is in the anti-gravity direction, and the water drop calibrator is provided with a calibration scale, when the bubble of the water drop calibrator is located at the uppermost scale of the circle, the direction of the positioning pile is parallel to the direction of gravity.

[0013] The above at least one technical scheme adopted by the embodiment of the application can achieve the following beneficial effects:

[0014] First, the device is used, the small animals are placed in supine position, the limbs are fixed, the tibialis anterior muscle belly part of one side of the lower limbs of the small animals is exposed through the shearing assembly, then the positioning assembly helps the shearing assembly to position the tibialis anterior muscle part and fix it, then the adjusting and measuring assembly is used to limit the size of the skeletal muscle injury range, so as to determine the range that needs to be sheared by the shearing assembly, finally the muscle is sheared through the shearing assembly, and then the muscle injury model is established, so that the effect of helping researchers to locate and quantify the establishment of the tibialis anterior muscle injury model of rodents is realized.

[0015] Secondly, when the tibialis anterior muscle of rodents is quantitatively injured, the fingers hold the pliers through the ring handle at the end of the holding part of the pliers, the two pliers are relatively far away through the rotating shaft, so that the arc-shaped blades provided on the abutting portions of the two pliers are relatively far away, so that the arc-shaped blades of the abutting portions of the two pliers are respectively placed on both sides of the tibia of the lower limbs that need to be sheared, and then the two pliers are relatively moved to the abutting position through the ring handle at the end of the holding part of the pliers, so that the arc-shaped blades fill the tibialis anterior muscle belly part, so that the tibialis anterior muscle belly part of one side of the lower limbs of the small animals is exposed, after the position and size of the muscle that needs to be sheared are determined, the pliers are held again through the ring handle, the arc-shaped blades of the two pliers are relatively far away, so that the two arc-shaped blades are respectively placed on both sides of the muscle that needs to be sheared, and then the two pliers are relatively moved to the abutting position through the ring handle, so that the arc-shaped blades fill the muscle and stop shearing, so that the shearing of the muscle that needs to be sheared is completed, the arc-shaped blade with a cross section of 0.6cm*0.3cm is provided, so that the shearing resistance is reduced during the shearing of the muscle, so that the pulling of the muscle caused by the pliers is reduced, the amount of sheared muscle is more accurate, the error of the amount of sheared muscle caused by pulling during shearing is avoided, and the experimental data is not accurate enough.

[0016] Thirdly, when quantitative shearing of the tibialis anterior muscle in rodents is required, firstly, insert one end of the positioning pin into the center of the patellar ligament of the small animal, and the other end into the anterior tendon of the ankle. Through the sleeve, both scales can slide at both ends of the sleeve, thereby lengthening or shortening the distance between the two positioning pins. This better helps the positioning pins on both sides to successfully insert into the central area of ​​the patellar ligament and the anterior tendon of the ankle. After fixing the position of the positioning pins, by aligning the scales at both ends of the sleeve, the curved blade can be positioned in the middle of the muscle to be sheared, ensuring that the muscle in the middle of the tibialis anterior muscle belly can be sheared. Then, remove the adjusting screw from the scale, allowing the scale to slide up and down within the groove of the positioning pin, thus... The forceps body slides up and down, allowing adjustment of the distance between the arc-shaped blade located at the stop of the forceps body and the muscle being cut. After adjustment, the adjusting screw is reinstalled at the end of the scale located outside the positioning post. The adjusting screw is fixed in the positioning post groove by the thread matching its threaded end, thus fixing the scale in the positioning post groove. The adjusting screws on both sides of the two positioning posts ensure a more stable fixation, preventing the forceps body from moving due to the scale sliding up and down during the cutting process, which would make it impossible to quantify the cut muscle. This achieves the effect of helping researchers to establish a rodent tibialis anterior muscle injury model by quantifying and locating the muscle, thus ensuring the accuracy of the experimental results.

[0017] Fourth, the angle controller makes it easier to control the angle at which the curved blade needs to cut when the two clamps rotate in opposite directions, thus making the amount of muscle cut more precise. At the same time, the angle scale teeth provide a certain amount of damping during the cutting process, and allow for more detailed control of the distance between the two clamps, thereby better controlling the cutting angle. In addition, the interaction between the angle scale teeth and the slots saves some of the force required to open the clamps, reducing fatigue during long-term use.

[0018] Fifth, during the cutting process of the curved blade, the depth of the curved blade entering the muscle can be adjusted by setting the blocking block, thereby better controlling and calculating the amount of muscle to be cut, thus making the experimental data more accurate.

[0019] Sixth, by setting up a water droplet calibrator, it is easier to achieve the parallelism between the positioning post and the direction of gravity, so that the positioning post is perpendicular to the direction of the tibialis anterior muscle, thus making it easier to place the muscle position cut by the arc blade in the middle of the belly of the tibialis anterior muscle. Attached Figure Description

[0020] The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:

[0021] Figure 1 Schematic view of the three-dimensional structure of the present application Figure One ;

[0022] Figure 2 Schematic view of the three-dimensional structure of the present application Figure Two ;

[0023] Figure 3 Schematic view of the three-dimensional structure of the positioning measuring device in the present application

[0024] Figure 4 Schematic view of the three-dimensional structure of the positioning measuring device in the present application

[0025] Figure 5 Schematic view of the three-dimensional structure of the shearing assembly in the present application

[0026] Reference signs

[0027] Shearing assembly 1, jaw body 11, rotating shaft 12, annular handle 13, arc-shaped blade 14, positioning measuring device 2, measuring assembly 21, sleeve 211, scale 212, adjusting screw 213, positioning assembly 22, positioning pile 221, pile needle 222, angle controller 3, angle scale tooth 4, blocking block 5, water drop calibrator 6. DETAILED DESCRIPTION

[0028] In order to make the objects, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described below in conjunction with specific embodiments of the present application and corresponding drawings. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0029] The technical solutions provided by the embodiments of the present application will be described in detail below in conjunction with the drawings.

[0030] The embodiment of the application provides a kind of tibia of rodent animal anterior muscle positioning quantitative injury model device, including shearing component 1 and positioning measuring device 2, the positioning measuring device 2 is arranged on shearing component 1, the positioning measuring device 2 includes the measuring component 21 for defining the size of skeletal muscle injury and the positioning component 22 for positioning tibia anterior muscle site, the measuring component 21 is arranged on positioning component 22, the measuring direction of the measuring component 21 is perpendicular to the positioning direction of positioning component 22, the positioning direction of the positioning component 22 is perpendicular to the shearing direction of shearing component 1 and the length of both ends of positioning component 22 is the output end of shearing component 1;When the device is used, first, small animals are placed in supine position, and their limbs are fixed, the tibia anterior muscle belly site of one side of lower limb of small animal is exposed by shearing component 1, then positioning component 22 helps shearing component 1 to position tibia anterior muscle site and make it fixed, then by adjusting measuring component 21, the size of skeletal muscle injury range is defined, so as to determine the range that shearing component 1 needs to shear, finally, muscle cutting is carried out by shearing component 1, and then muscle injury model is established, so as to achieve the effect that rodent animal tibia anterior muscle muscle injury model can be established by positioning and quantification for researchers;The device achieves the effect that rodent animal tibia anterior muscle muscle injury model can be established by positioning and quantification for researchers.

[0031] Preferably, the shearing assembly 1 comprises two pincer bodies 11, a rotating shaft 12, a ring-shaped handle 13 and an arc-shaped blade 14, the two pincer bodies 11 are cross arranged and the contact ends thereof are rotatably connected through the rotating shaft 12, the pincer body 11 comprises a stop portion and a holding portion, the stop portion of each of the two pincer bodies 11 is provided with the arc-shaped blade 14 and symmetrically arranged, and the end of the holding portion of each of the two pincer bodies 11 is provided with the ring-shaped handle 13 for fingers to pass through; when quantitatively damaging the tibialis anterior muscle of a rodent, first, the fingers pass through the ring-shaped handle 13 at the end of the holding portion of the pincer body 11 to hold the pincer body 11, and the stop portions of the two pincer bodies 11 are relatively away from each other through the rotating shaft 12, so that the arc-shaped blades provided on the stop portions of the two pincer bodies 11 are relatively away from each other, so that the arc-shaped blades on the stop portions of the two pincer bodies 11 can be respectively placed on both sides of the tibia of the lower limb which needs to be sheared, and then the stop portions of the two pincer bodies 11 are relatively moved to the stop position through the ring-shaped handle 13 at the end of the holding portion of the pincer body 11, so that the arc-shaped blades 14 fill the tibialis anterior muscle belly part, thereby completing the exposure of the tibialis anterior muscle belly part on one side of the lower limb of the small animal, and after determining the position and size of the muscle which needs to be sheared, the pincer body 11 is held again through the ring-shaped handle 13, so that the arc-shaped blades of the two pincer bodies 11 are relatively away from each other, so that the arc-shaped blades are respectively placed on both sides of the muscle which needs to be sheared, and then the two pincer bodies 11 are relatively moved to the stop position through the ring-shaped handle 13, so that the arc-shaped blades 14 fill the muscle and stop shearing, thereby completing the shearing of the muscle which needs to be sheared, and through the arc-shaped blade 14 with a cross section of 0.6cm*0.3cm, the shearing resistance in the process of shearing the muscle is reduced, so that the pulling of the cut muscle on the pincer body 11 is reduced, the amount of sheared muscle is more accurate, the amount of sheared muscle due to pulling during shearing is avoided, and the experimental data is not accurate enough.

[0032] Preferably, the measuring assembly 21 comprises a sleeve 211, a scale 212 and an adjusting screw 213, the sleeve 211 penetrates through the contact ends of the two pincer bodies 11 which are cross arranged and is sleeved on the rotating shaft 12, the scale 212 is in a cylindrical shape, the scale 212 is provided with two, the two scales 212 are respectively symmetrically arranged inside the two ends of the sleeve 211 and are in sliding fit with the sleeve 211, and the adjusting screw 213 is provided with two, one end of each scale 212 away from the sleeve 211 is detachably sleeved with the adjusting screw 213 and the two adjusting screws 213 are symmetrically arranged.

[0033] Preferably, the positioning assembly 22 comprises positioning stakes 221 and stake needles 222, the positioning stakes 221 are provided with two, and the two positioning stakes 221 are vertically sleeved at the contact ends of the two scales 212 and the adjusting screws 213 and are in sliding fit with them, the two adjusting screws 213 are located on the sides of the two positioning stakes 221 away from the sleeve 211, and the two positioning stakes 221 are both provided with sliding grooves for the up-and-down sliding of the scales 212, and the sliding grooves are both provided with threads matched with the threaded ends of the adjusting screws 213, the stake needles 222 are provided with two, and the two stake needles 222 are both arranged at the bottoms of the positioning stakes 221 and are arranged with the needle tip ends downward, and the needle tip ends of the two stake needles 222 are located on the same horizontal line; when the tibialis anterior muscle of a rodent needs to be quantitatively cut, first, the stake needle 222 at one end of the positioning stake 221 is inserted into the central patellar ligament of the small animal, and the stake needle 222 at the other end is inserted into the anterior tendon of the ankle of the small animal, the sleeve 211 is arranged, so that the two scales 212 can slide at the two ends of the sleeve 211, thereby the distance between the two positioning stakes 221 can be lengthened or shortened, so that the two positioning stakes 221 can be better inserted into the central patellar ligament and the anterior tendon of the ankle, after the positions of the positioning stakes 221 are fixed, the scales at the two ends of the sleeve 211 are made consistent, so that the arc-shaped blade 14 can be located at the middle position of the muscle to be cut, and the muscle at the middle position of the tibialis anterior muscle belly can be ensured to be cut, then the adjusting screw 213 is detached from the scale 212, so that the scale 212 can slide up and down in the sliding groove of the positioning stake 221, thereby the forceps body 11 can slide up and down, so that the distance between the arc-shaped blade 14 at the abutting portion of the forceps body 11 and the muscle to be cut can be adjusted, after the adjustment is completed, the adjusting screw 213 is reattached to the scale 212 at the end of the positioning stake 221 outside, the thread matched with the threaded end of the positioning stake 221 is arranged in the sliding groove of the positioning stake 221, so that the adjusting screw 213 is fixed in the sliding groove of the positioning stake 221, thereby the scale 212 is fixed in the sliding groove of the positioning stake 221, the adjusting screws 213 are arranged on the two sides of the two positioning stakes 221, so that they are fixed more stably, and the up-and-down sliding of the scale 212 during the cutting process is avoided, so that the cut muscle cannot be quantified, thereby the effect of helping researchers to locate and quantify the establishment of a rodent tibialis anterior muscle injury model is achieved, and the accuracy of the experimental results is ensured.

[0034] Preferably, one of the holding portions of the two clamping bodies 11 is provided with an angle controller 3 near one end of the annular handle 13, the angle controller 3 is provided with an angle scale tooth 4 near one side of the annular handle 13 and is fixedly connected with the angle scale tooth 4, the holding portion of the other clamping body 11 is provided with a slot hole through which the angle controller 3 and the angle scale tooth 4 pass, the slot hole is provided with an opening complementary to the shape of the angle controller 3, and the angle controller 3 and the angle scale tooth 4 are slidingly fitted in the slot hole; through the angle controller 3, when the two clamping bodies 11 are crossed and rotated, the angle at which the arc-shaped blade 14 needs to be sheared is more convenient to control, so that the amount of muscle sheared is more accurate, and through the angle scale tooth 4, a certain damping feeling is provided for the shearing process, and the distance between the two clamping bodies 11 can be controlled in more detail, so that the angle during shearing is better controlled, and through the cooperation between the angle scale tooth 4 and the slot hole, part of the force required when the clamping body 11 is opened is saved, and the fatigue feeling after long-time use is reduced.

[0035] Preferably, the angle controller 3 and the angle scale tooth 4 are provided with a blocking block 5, the blocking block 5 is provided with two, and the two blocking blocks 5 are respectively located on the two sides of one of the clamping bodies 11; during the shearing process of the arc-shaped blade 14, the depth of the arc-shaped blade 14 into the muscle can be adjusted through the blocking block 5, so that the amount of muscle required to be sheared is better controlled and calculated, and the data of the experimental results is more accurate.

[0036] Preferably, the positioning stake 221 is provided with a water drop calibrator 6, the water drop calibrator 6 is spherical, is filled with water, and is provided with a bubble in the water, the bubble is in the anti-gravity direction, the water drop calibrator 6 is provided with a calibration scale, when the bubble of the water drop calibrator 6 is located at the uppermost scale of the circle, the direction of the positioning stake 221 is parallel to the direction of gravity; through the water drop calibrator 6, the parallelism between the positioning stake 221 and the direction of gravity is better realized, so that the positioning stake 221 is perpendicular to the direction of the tibialis anterior muscle, and the muscle position sheared by the arc-shaped blade 14 is located in the middle of the tibialis anterior muscle belly.

[0037] The above only describes the embodiments of the present application and is not used to limit the present application. The present application can have various changes and modifications for those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the scope of claims of the present application.

Claims

1. A rodent tibialis anterior muscle localization and quantitative injury model device, characterized in that, The utility model provides a cutting assembly (1) and positioning measurement device (2) are included, positioning measurement device (2) sets up on cutting assembly (1), positioning measurement device (2) includes the measurement component (21) for defining the skeletal muscle damage size and the positioning component (22) for positioning tibialis anterior muscle site, the measurement component (21) sets up on positioning component (22), the measurement direction of measurement component (21) is perpendicular to the positioning direction of positioning component (22), the positioning direction of positioning component (22) is perpendicular with the cutting direction of cutting assembly (1) and the both ends length of positioning component (22) is with the output end of cutting assembly (1);The cutting assembly (1) includes the jaw body (11), the rotating shaft (12), the annular handle (13) and the arc blade (14), the jaw body (11) is equipped with two, two jaw body (11) are arranged in intersection and are connected by rotating shaft (12) rotationally in contact end, the jaw body (11) includes the abutment and the holding part, the abutment of two jaw body (11) is equipped with arc blade (14) and is symmetrically arranged, and the holding part end of two jaw body (11) is equipped with annular handle (13) for finger to pass through;The measurement component (21) includes sleeve (211), scale (212) and adjusting screw (213), sleeve (211) is through in two jaw body (11) intersection arrangement contact end and is set on rotating shaft (12), scale (212) is cylindrical, scale (212) is equipped with two, two scale (212) are symmetrically arranged respectively in the inside of sleeve (211) both ends and with its sliding fit, adjusting screw (213) is equipped with two, every scale (212) is removed from the one end of sleeve (211) and is detachably set with adjusting screw (213) and two adjusting screws (213) are symmetrically arranged;The positioning component (22) includes the locating pile (221) and the pile needle (222), the locating pile (221) is equipped with two, two locating piles (221) are respectively vertically set in two scale (212) and adjusting screw (213) contact end and with its left and right sliding fit, two adjusting screws (213) are all located in the one side of two locating piles (221) away from sleeve (211), two locating piles (221) are all set up with the sliding slot of letting scale (212) slide up and down, the sliding slot inside is all equipped with the screw thread matched with the screw thread end of adjusting screw (213), the pile needle (222) is equipped with two, two pile needles (222) are all set in the bottom of locating pile (221) and the needle tip end is set down, and the needle tip end of two pile needles (222) is located on the same horizontal line.One of the holding part of the jaw (11) near the ring handle (13) one end is provided with angle controller (3), the angle controller (3) near the ring handle (13) one side is provided with angle scale tooth (4) and is fixedly connected with it, the holding part of the other jaw (11) is provided with the slot hole corresponding to angle controller (3) and angle scale tooth (4) passes through, the slot hole is provided with the opening complementary with the shape of angle control tooth, and the angle controller (3) and angle scale tooth (4) are slidingly fitted in the slot hole.

2. The tibialis anterior muscle positioning and quantitative injury model device for rodents according to claim 1, characterized in that, The angle controller (3) is sleeved with a blocking block (5) on the angle scale tooth (4), the blocking block (5) is provided with two, and the two blocking blocks (5) are located on the two sides of one jaw body (11) respectively.

3. The tibialis anterior muscle positioning and quantitative injury model device for rodents according to claim 1, characterized in that, The positioning pile (221) is provided with a water drop calibrator (6), the water drop calibrator (6) is spherical, the inside is water, the water is provided with a bubble, the bubble is in the direction of countergravity, the water drop calibrator (6) is provided with a calibration scale, when the bubble of the water drop calibrator (6) is located at the uppermost scale of the circle, the direction of the positioning pile (221) is parallel to the direction of gravity.

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